Eppendorf Research® plus

Eppendorf Research® plus
Operating manual
Operating manual
Eppendorf Research® plus
Research plus - Operating Manual - en
Operating manual
Copyright© 2010 Eppendorf AG, Hamburg. No part of this publication may be reproduced without
the prior permission of the copyright owner.
Trademarks
Combitips®, eppendorf®, epT.I.P.S.®, Multipette®, Repeater® and Research® are registered
trademarks of Eppendorf AG, Hamburg, Germany.
Registered trademarks are not marked in all cases with ™ or ® in this manual.
U.S. Pat. No.
5,531,131
4,961,350
For user adjustment with adjustment display, spring to reduce force for tip fitting and for further
features patents are pending.
Research plus - Operating Manual - en-02/0210
Eppendorf Research® plus — Operating manual
Table of contents
Table of contents
Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.1 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.3 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.3.1 Research plus single-channel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.3.2 Research plus multi-channel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.4 Materials. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
4
4
4
5
5
6
7
2
Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.1 Intended use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.2 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
3
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3.1 Setting the volume (only Research plus variable) . . . . . . . . . . . . . . . . . . . . . . . . . . .
3.2 Using pipette tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3.3 Aspirating liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3.4 Dispensing liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
4
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
4.1 Error search . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
5
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.2 Sterilizing or disinfecting the pipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.2.1 Autoclaving . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.2.2 Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.3 Replacing O-rings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.3.1 Removing the O-ring. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.3.2 Mounting a new O-ring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.4 Research plus disassembly and assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.4.1 Single-channel up to 1 000 μL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.4.2 5 mL and 10 mL single-channel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.4.3 Multi-channel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.5 Adjusting pipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.5.1 General notes on user and factory adjustment settings . . . . . . . . . . . . . . . . .
5.5.2 Changing the user adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.5.3 Changing the factory adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.5.4 Research plus variable - changing the factory adjustment . . . . . . . . . . . . . . .
5.5.5 Error limits in accordance with ISO 8655-2. . . . . . . . . . . . . . . . . . . . . . . . . . .
5.6 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
14
14
15
15
16
16
16
16
17
17
19
20
22
22
24
25
25
31
34
6
Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
6.1 Research plus single-channel variable. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
6.2 Research plus multi-channel variable. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
6.3 Research plus fix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
6.4 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
35
35
37
38
39
7
Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Table of contents
1
10
10
10
11
11
3
Eppendorf Research® plus — Operating manual
1 Product description
1
1.1
Product description
Features
The Research plus pipettes are piston-stroke pipettes that operate according to the air-cushion
principle.
When the control button is pressed, the piston in the pipette moves in the same direction. If the
control button and the piston are moved upwards, the liquid can be aspirated into the pipette tip.
With the downward movement of the piston, the liquid is dispensed (measuring stroke).
Blow-out occurs if the downward piston stroke exceeds the first stop.
Depending on the Research plus pipette it is possible to dispense volumes from 0.1 μL to 10 mL.
The Research plus family consists of single-channel and multi-channel pipettes (8 and
12-channels) with variable volume settings, as well as single-channel fixed-volume pipettes. You
can find a list of all available models of the Research plus in the "Technical Data" section.
All Research plus pipettes are fully autoclavable.
You can adjust the Research plus pipettes. The side viewing window displays the change in the
adjustment.
When attaching a pipette tip, the spring-loading action of the tip cone is activated (exceptions
5 mL and 10 mL pipettes).
Product description
1
1.2
Delivery package
The Research plus delivery package contains:
4
Pcs.
Description
1
Research plus operating manual
1
Certificate
1
Adjustment tool (Allen key with a blue handle)
1
Mini CD
1
Black locking ring for single-channel pipettes ≤ 1,000 μL
5
Red adjustment seal
5
Protective filters for 5 mL and 10 mL pipettes
1
Pipette key for opening the lower part (5 mL and 10 mL)
1
O-ring tool for cutting the O-rings (only multi-channel 100 μL and 300 μL)
1
Safety plug tool
1
Pin for loosening the safety plug
Eppendorf Research® plus — Operating manual
1 Product description
1.3
1.3.1
Main illustration
Research plus single-channel
1
2
1 Control button
The control button and the trays of the matching epT.I.P.S. pipette
tips have the same color.
2 Volume adjustment ring
To set the volume for the variable pipettes.
4
3 Ejector
The ejector moves the ejector sleeve and ejects the pipette tip.
5
6
7
8
Product description
3
1
4 Volume display (only variable pipettes)
The set volume is read from top to bottom.
5 Adjustment opening
The adjustment opening is fitted with the gray adjustment seal
before delivery.
6 Adjustment display
Set to "0" before delivery.
7 Labeling field
Space for labels containing internal lab information. The serial
number appears at the bottom.
9
10
8 Ejector sleeve
Eject the pipette tips after use.
9 Spring-loaded tip cone
The spring loading action optimizes the force required for
attaching and ejecting tips (no spring-loaded action with 5 mL
and 10 mL pipettes). The 5 mL and 10 mL pipettes have an
easily replaceable protection filter in the tip cone.
10 Pipette tip
The Research plus pipettes can only be used in combination with
matching pipette tips. It is recommended to use epT.I.P.S.
5
Eppendorf Research® plus — Operating manual
1 Product description
1.3.2
Research plus multi-channel
For an explanation of the upper part of the pipette,
refer to the main illustration of the single-channel
pipette (see p. 5).
1
Product description
1 Lever
To loosen the multi-channel lower part.
6
1
2
3
4
5
2 Multi-channel lower part
The multi-channel lower part is freely rotatable. The
lower part does not detach when it is rotated. The
outer channels are labeled with the numbers 1 and 8
(or 12).
The multi-channel version has a piston for each
channel so that fewer than 8 or 12 tips can also be
fitted.
The lower part can be converted from an 8 to a
12-channel version with the same volume range.
The multi-channel lower part can be opened,
enabling you to replace or remove individual
channels.
3 Latches on the right and left
To release the cover plate with the interior ejector
rail.
4 Spring-loaded tip cones
The spring loading action optimizes the force
required for attaching and ejecting tips.
5 Pipette tips
It is recommended to use epT.I.P.S.
6 Cover plate
Detachable cover plate with an interior ejector rail.
The cover plate is opposite the side with the
lever (1). In the view shown here, the cover plate is
the rear side of the lower part.
6
Eppendorf Research® plus — Operating manual
1 Product description
1.4
CAUTION!
Materials
CAUTION! Aggressive substances can damage the device, dispensing unit and
accessories.
Check for material compatibility before using organic solvents and aggressive
chemicals.
Follow the cleaning instructions (see Cleaning on p. 14).
1
Component
Material
External surfaces of the upper part
Purified polypropylene (PP),
polycarbonate (PC),
polyetherimide (PEI),
foil
Exterior and interior of lower parts
Purified polypropylenen (PP),
polyvinylidene fluoride (PVDF),
polyetherimide (PEI),
polyphenylene sulfide (PPS),
polyetheretherketone (PEEK),
polytetrafluorethylene (PTFE),
ethylene-propylene-diene rubber (EPDM),
silicone,
steel (stainless steel and spring steel)
Pipette tip
Material
epT.I.P.S.
Polypropylene (PP)
epDualfilter T.I.P.S. filter
Polyethylene (PE)
Product description
The user-accessible components of the Research plus are made of the following materials:
7
Eppendorf Research® plus — Operating manual
2 Safety
2
2.1
Safety
Intended use
The Research plus is a lab device intended for dispensing liquids in the volume range from
0.1 μL to 10 mL, in combination with matching pipette tips. In vivo applications (applications in or
on the human body) are not permitted.
The Research plus may only be operated by trained specialist staff. All users must have read the
operating manual carefully and familiarized themselves with the device's mode of operation.
2.2
Warnings for intended use
2
Safety
WARNING!
WARNING!
CAUTION!
CAUTION!
NOTICE!
8
WARNING! Damage to health due to handling infectious liquids and pathogenic
germs.
Observe the national regulations for handling these substances, the biological
security level of your laboratory, the material safety data sheets and the
manufacturer's application notes.
Wear personal protective equipment (PPE).
Follow the instructions regarding hygiene, cleaning and decontamination.
For complete instructions regarding the handling of germs or biological material of
risk group II or higher, please refer to the "Laboratory Biosafety Manual" (source:
World Health Organization, current edition of the Laboratory Biosafety Manual).
WARNING! Damage to health due to toxic, radioactive or aggressive chemicals.
Observe the national regulations for handling these substances as well as the
material safety data sheets and manufacturer's application notes.
Wear personal protective equipment (PPE).
CAUTION! Danger to persons from careless use.
Never point the opening of a Research plus fitted with pipette tip at yourself or
anyone else.
Only initiate dispensing if it is safe to do so.
For all dispensing tasks, make sure that you are not endangering yourself or
anyone else.
CAUTION! Poor safety due to incorrect accessories and spare parts.
The use of accessories and spare parts other than those recommended by Eppendorf
may impair the safety, functioning and precision of the device. Eppendorf cannot be
held liable or accept any liability for damage resulting from the use of incorrect or
non-recommended accessories and spare parts or from the improper use of such
equipment.
Only use accessories and original spare parts recommended by Eppendorf.
NOTICE! Damage to device from missing pipette tips.
Only use the Research plus with fitted pipette tips.
When using standard tips (without a filter): use the 1 - 10 mL and 0.5 - 5 mL
pipettes only with the protection filter inserted.
Eppendorf Research® plus — Operating manual
2 Safety
NOTICE!
NOTICE!
NOTICE! Carry-over, contamination and incorrect dispensing results due to the
incorrect use of pipette tips.
The pipette tips are for single use only. Prolonged use can have a negative impact on
dispensing tasks.
Use the pipette tips only once.
Do not autoclave the epT.I.P.S. Dualfilter.
2
Safety
NOTICE! Incorrect dispensing volume for special liquids and from temperature
differences.
Solutions which differ greatly from water in terms of their physical data, or temperature
differences between the pipette, pipette tip and liquid can result in incorrect dispensing
volumes.
Avoid temperature differences between pipette, pipette tip and liquid.
Make sure that the temperature is constant, between 20 and 25°C and at ±0.5°C.
Check the dispensing volume and readjust the pipette in case of deviations.
9
Eppendorf Research® plus — Operating manual
3 Operation
3
3.1
Operation
Setting the volume (only Research plus variable)
Turn the volume setting ring as depicted to adjust the
volume.
The height of the control button changes as the volume is
adjusted.
The numbers on the volume display are to be read from
the top to the bottom. The decimal places are below the
hyphen.
The volume is displayed up to 1 000 μL in μL. For the 5 mL
and 10 mL sizes, the volume is displayed in mL.
We recommend to adjust the volume setting from a higher
value to a lower value. If required, turn beyond the
required value and then back again.
3
3.2
Using pipette tips
Operation
The liquid to be dosed is aspirated into pipette tips. It is recommended to use epT.I.P.S. The
epT.I.P.S. pipette tips are available in different purities, as tips with and without a filter and as
special tips. When using liquids whose surface tension is lower than that of water (e.g., due to the
presence of a wetting agent in the liquid), the use of the special tips epT.I.P.S. LoRetention is
recommended.
The pipette is only functional if a pipette tip has been
attached. You can either attach the pipette tip by hand or
directly insert the end of the pipette into a tip held in the tip
storage box. If attaching a pipette tip by hand, it must be
handled in such a way to avoid contamination and heating of
the pipette tip.
The color of the control button of the Research plus matches
the color of the epT.I.P.S. tray.
If you are using pipette tips without a filter, insert the
protection filter into the 5 mL and 10 mL pipettes.
If you are using the 5 mL or 10 mL epDualfilter T.I.P.S.,
remove the protection filter in the pipette. This also
applies to 5 mL / 10 mL filter tips of other manufacturers.
The filters can interfere with each other. The
backpressure of the two filters makes it difficult to exactly
identify the first stop (see p. 11).
Fit the suitable pipette tip(s) on the tip cone, applying light
pressure.
The pipette tip is securely attached to the tip cone when
it responds with spring-loaded action (exceptions: no
spring-loading action for 5 mL and 10 mL single-channel
pipettes)
You can deactivate the spring loading action of the tip cone in
the case of single-channel pipettes (see p. 17).
10
Eppendorf Research® plus — Operating manual
3 Operation
3.3
Aspirating liquid
1. The liquid to be aspirated must be taken from a suitable vessel. For
multi-channel pipettes, we recommend the reagent reservoir "Tip-Tub".
2. Press down the control button to the first stop (measuring stroke).
3. Immerse the pipette tip(s) vertically approx. 4 mm into the liquid.
4. To aspirate liquid, allow the control button to slide back slowly. Maintain the
immersion depth, so that no air is aspirated accidentally.
5. In the case of large volumes: before removing the pipette tip from the liquid,
wait for approx. 3 seconds. To ensure maximum precision and accuracy, we
recommend to wet each new tip initially by aspirating and dispensing the
liquid one to three times. Only then should pipetting commence.
6. Remove the tip(s) slowly from the liquid.
7. Wipe the tip(s) slowly against the tube wall to ensure that no outer wetting
remains on the tip.
3.4
3
Dispensing liquid
Operation
1. Place the tip(s) on the tube wall at an angle.
2. Press the control button slowly until the first stop (measuring stroke) and
wait until the flow of liquid stops.
3. To empty the tip(s) completely, press down the control button until the
second stop (blow-out).
4. Hold down the control button and wipe the tip(s) against the tube inner wall.
5. Let the control button slide back slowly outside of the tube.
6. To eject the tips, press the ejector.
Pipette tips are for single use only.
11
Eppendorf Research® plus — Operating manual
4 Troubleshooting
4
4.1
Troubleshooting
Error search
Symptom
Liquid is dripping from
the tip and/or the
dispensed volume is
incorrect.
Possible cause
Solution
• The tip is loose or the
Press the tip on firmly, use
• Liquid with high vapor
Wet the tip several times and
• Pipetted too quickly.
Move the control button slowly.
pipette tip is poorly fitted.
pressure and/or different
density.
epT.I.P.S. If using 5 mL and
10 mL epDualfilter T.I.P.S., do
not use protection filters in the
pipette.
adjust the pipette for the liquid
used.
• The tip is withdrawn from Slowly remove the tip with a
the liquid too quickly.
4
• Liquid aspirated with
Repeat dispensing correctly.
• The piston is soiled or
Clean the piston, relubricate
blow-out and dispensed
with blow-out.
damaged.
Troubleshooting
time delay (approx. 3 seconds)
from the liquid.
slightly and/or replace.
• The tip cone is damaged. Replace the lower part or
channel.
• The O-rings of the tip
Replace the O-rings (only
The control button jams
and does not move
smoothly.
• The piston is soiled.
• The seal is soiled.
• The pipette is blocked.
Clean the lower part.
5 mL and 10 mL sizes: replace
The adjustment seal has
been removed; the
adjustment display has
been changed.
• The pipette has been
Adjust the pipette for the liquid
No spring-loading action
of the tip cone when
taking up pipette tips.
• Spring-loading action is
Remove the locking ring again.
• The use of a 5 mL or
No remedy. The tip cone does not
respond with spring-loaded action in
combination with these sizes.
cones are damaged.
adjusted for another
liquid.
blocked by a locking ring.
10 mL pipette.
12
100 μL, 300 μL multi-channel).
the protection filter.
used (see Adjusting pipettes on
p. 22).
Eppendorf Research® plus — Operating manual
4 Troubleshooting
To avoid dispensing errors, check the precision and accuracy of the Research plus at regular
intervals. The "PICASO" program (from Version 2.3) is available to determine the permitted
measured systematic and random errors. If the pipette is used at an extremely high location, it
must be adjusted to the ambient air pressure. A SOP (Standard Operation Procedure) for
checking pipettes can be found on the Research plus CD and on our website
www.eppendorf.com.
4
Troubleshooting
13
Eppendorf Research® plus — Operating manual
5 Maintenance
5
Maintenance
5
Maintenance
NOTICE!
NOTICE! Aggressive substances can damage the pipette, pipette tips and the
accessories.
Check for material compatibility before using organic solvents and aggressive
chemicals.
Follow the cleaning instructions.
• All single and multi-channel lower parts are wear parts. Clean them after contamination, use
•
of aggressive chemicals and/or heavy stress. If the lower parts are worn or damaged, replace
the respective parts.
Faulty dispensing results are sometimes due to lack of maintenance.
5.1
NOTICE!
Cleaning
NOTICE! Damage to device from unsuitable cleaning fluids or sharp or pointed
objects
Unsuitable cleaning fluids can damage the surfaces and printing.
Never use corrosive cleaning fluids, strong solvents or abrasive polishes.
Note the information on chemical resistance (see the Research plus CD).
Do not use acetone to clean the Research plus.
Do not use sharp objects to clean the Research plus.
Remove any contamination on the outside of the Research plus as follows:
Wet a cloth with a mild cleaning fluid and water and remove the contamination.
To remove heavy contamination resulting from liquid penetration, disassemble the lower part
of the pipette (see Research plus disassembly and assembly on p. 17) and clean it with
demineralized water.
Relubricate the piston sealing rings after contamination, use of aggressive chemicals and/or
heavy stress. Remove the old grease before relubricating.
Only use the grease specified in the ordering information (see the enclosed CD).
14
Eppendorf Research® plus — Operating manual
5 Maintenance
5.2
5.2.1
NOTICE! Damage to device from incorrect handling.
Do not use any additional disinfectants, decontamination agents or sodium
hypochlorite during autoclaving or UV exposure.
When autoclaving make sure that the temperature does not exceed 121°C.
Before using disinfecting agents or decontaminating agents, test for material
compatibility and check the manufacturer's instructions about chemical resistance.
Consider also the material of the pipette.
Maintenance
NOTICE!
5
Sterilizing or disinfecting the pipette
Autoclaving
All Research plus pipettes are completely steam autoclavable.
Before autoclaving
1. Remove any contamination from the exterior and in the lower part (see Research plus
disassembly and assembly on p. 17).
2. If you remove existing grease, slightly relubricate the piston seal using only the grease
specified in the ordering information (see the enclosed CD).
Procedure
Follow the operating manual of the autoclave manufacturer.
1. Autoclave at: 121°C; 20 minutes; 1 bar overpressure
2. When placing the Research plus into the autoclave, make sure that the temperature at the
pipette does not exceed 121°C.
3. You can put the Research plus into the autoclave as a whole unit or with the lower part
removed. Do not disassemble the lower part.
4. For 5 mL and 10 mL pipettes: remove the old protection filter. Add a new protection filter and
install it after autoclaving. Autoclave the protection filter only once.
5. If lower parts have been removed, make sure that no lower parts are confused during
reassembly. (Tip: Use one plastic beaker per pipette).
After autoclaving
Cool the pipette down to room temperature and leave to dry.
For 5 mL / 10 mL pipettes: the protection filter swells during autoclaving. Slightly compress
the protection filter when inserting it into the tip cone.
The piston does not need to be relubricated after autoclaving. If autoclaving is carried out as
described above, no gravimetric testing or adjustment is necessary.
15
Eppendorf Research® plus — Operating manual
5 Maintenance
5
5.2.2
Disinfection
Carefully wipe off the outer surfaces with disinfectant, DNA/RNA decontamination agents or
70% isopropanol.
Maintenance
You can wipe the outside of the tip cone and the ejector sleeve with a sodium hypochlorite
solution of 4%.
After the exposure time of the sodium hypochlorite solution has elapsed, remove thoroughly
with demineralized water.
The Research plus pipettes may be temporarily subjected to the UV light of a sterile bench ( ≥254
nm).
5.3
Replacing O-rings
The 100 μL and 300 μL multi-channel lower parts are equipped with O-rings. These are wear
parts. Replace old, worn or damaged O-rings. Defective O-rings result in the incorrect positioning
of the tips and in dispensing errors.
5.3.1
Removing the O-ring
1. Push the opening of the O-ring tool (included in the delivery package) against the tip cone
from the side so that the sharp edge in the opening of the O-ring tool is positioned on top of
the o-ring. Do not slide the O-ring tool onto the cone!
2. Supporting the O-ring tool with your thumb, push it firmly against the tip cone.
This O-ring is cut in one place.
3. Remove the O-ring tool and the O-ring from the tip cone.
5.3.2
Mounting a new O-ring
1. Fit the mounting aid (shortened pipette tip, included in the delivery package of the O-rings)
on the tip cone.
2. Push the new O-ring over the tip onto the tip cone.
3. Check that the pipette tips are correctly positioned. Ensure that the tips are tightly sealed and
properly aligned.
16
Eppendorf Research® plus — Operating manual
5 Maintenance
5.4
5.4.1
5
Research plus disassembly and assembly
Single-channel up to 1 000 μL
Maintenance
Removing the lower part
PUSH UP TO RELEASE
1
2
3
4
1. Keep the ejector pressed and remove the ejector sleeve 1 .
2. 2 and 3 : on the lower part, slide the ring marked "PUSH UP TO RELEASE" up by about
5 mm until the lower part comes off.
3. 4 : take the lower part out of the upper part.
Deactivating the spring-loading action: installing the locking ring
For the following dispensing tasks it can be helpful to deactivate the spring loading action of the
tip cone.
• A pipette tip is to be used for a long period.
• The pipette tip is slightly bended during dispensing.
Deactivating this action can also be helpful for pipette tips which are not designed for the
Eppendorf pipette tip cone.
The spring loading action of the tip cone can be deactivated by installing
the locking ring. The locking ring is included in the delivery package.
1. Push the black locking ring onto the lower part from the top. Slightly
squeeze the clamps on the lower part when doing so.
2. Insert the lower part into the upper part until it engages audibly.
3. Keep the ejector pressed. The ejector rod protrudes from the upper
part.
4. Fit the ejector sleeve onto the ejector rod. It is fitted correctly if it
engages slightly.
Remove the locking ring to reactivate the spring-loading action.
17
Eppendorf Research® plus — Operating manual
5 Maintenance
5
Opening and assembling the lower part (≤1 000 μL)
To avoid confusion of parts, only disassemble and assemble one pipette at a time.
Maintenance
Hint!
1
2
3
4
Opening the lower part:
1. On the piston mount (1) push the locking mechanisms
together slightly.
2. Remove the piston mount.
3. Remove the piston (2) and the piston spring (3). The
piston is under spring tension. Pistons and piston springs
will look different for the individual volume versions.
Assembling the lower part:
1. Carefully guide the piston and the piston spring into the
cylinder. Make sure that the piston is guided correctly in
the piston spring and in the cylinder. There must not be
any perceptible resistance. Stop pushing as soon as
any resistance is felt This may indicate that the piston is
not positioned correctly in the cylinder. There is a risk of
bending the piston if too much force is applied. Carefully
pull out the piston and repeat the process correctly. In the
case of piston springs with double windings (4), these
windings must point downwards.
2. Keep the piston and the piston spring pressed.
3. Keep the locking mechanisms on the piston mount
pressed with the other hand.
4. Mount the piston mount in such a way that the two locking
mechanisms engage in their retainers.
5. Gently press a pipette tip against the inserted piston. The
piston must move down in the cylinder without any
noticeable resistance.
Installing the lower part and ejector sleeve:
1. Insert the lower part into the upper part until it engages
audibly.
2. Set the maximum volume and operate the control button
several times. It must run smoothly and resistance-free.
3. Keep the ejector pressed. The ejector rod protrudes from
the upper part.
4. Fit the ejector sleeve onto the ejector rod. It is fitted
correctly if it engages slightly.
5. Carry out a gravimetric test of the systematic and random
error (see Technical data on p. 35).
This test ensures that no parts were confused during
assembly and that the pipette has been assembled correctly.
18
Eppendorf Research® plus — Operating manual
5 Maintenance
5.4.2
5
5 mL and 10 mL single-channel
Replace the protection filter in the tip cone after each contact with liquid or if it is frayed.
Maintenance
4
1
30°
2
2
1
1.
2.
3
: unscrew the ejector sleeve.
: keep the ejector pressed and turn the lower part to the left or right by approximately 30°.
The lower part is automatically released from the upper part.
3. 3 : open the lower part: fit the pipette key 4 (included in the delivery package) onto the lower
part. Hold the cylinder and unscrew it from the lower part.
See the next figure.
4. Hold the piston (4) which is now partly visible in the lower part.
5. Push the piston mount (1) slightly downwards (3 mm) and turn it by 45°.
The piston spring pushes the piston mount upwards. The lower part opens.
The lower part consists of the following:
1
2
1
2
3
4
5
6
1 5 mL / 10 mL piston mount
2 Piston spring
3 Upper part of the lower part
4 Piston (with seal)
5 Cylinder and tip cone
6 Protection filter in the tip cone
19
Eppendorf Research® plus — Operating manual
5 Maintenance
5
Maintenance
Before assembling the pipette, first install the piston mount, the piston spring and the piston in the
upper part of the lower part. Further assembly is as shown in the figures. Carry out the procedure
in reverse order. After the assembly:
The assembly is performed in reverse order. After the assembly:
Carry out a gravimetric test of the systematic and random error (see Technical data on
p. 35).
This test ensures that no parts were confused during assembly and that the pipette has been
assembled correctly.
5.4.3
Multi-channel
Loosen and open the 10 μL, 100 μL and 300 μL multi-channel lower part
1
1.
2
1 and 2 : slide the lever on the lower part to the left or right.
This separates the lower part from the upper part so that it can be removed.
2. Put down the lower part with the lever facing downwards.
3. 3 : slide the two latches (right and left at the side) down. Use a coin to do this.
The lower part is still lying with the lever facing downwards.
4. Take off the cover plate with the integrated ejector rail.
Do not remove the ejector rail from the cover plate. The spring for the ejector rail could
accidentally come off and get lost.
20
3
Eppendorf Research® plus — Operating manual
5 Maintenance
5
Removing and installing channels
1
1.
2.
3.
4.
5.
6.
2
3
4
Maintenance
Channels must only be removed and installed if the multi-channel lower part is separated from
the upper part! The channels in the lower parts consist of a piston, a cylinder and a spring. The
channels for 100 μL and 300 μL are equipped with an O-ring at the tip cone (see Replacing
O-rings on p. 16).
5
1 and 2 : position a pipette tip under the piston and carefully take the piston off the upper
rail.
Carefully pull the piston out in an upward direction. Do not bend the piston.
Hold the tip cone at the lower end and push it slightly upwards 3 .
This compresses the spring.
4 : lift the tip cone slightly and release it from the lower rail.
5 : relax the spring by letting the tip cone slide back again above the lower rail.
5 : take the tip cone with the cylinder and the spring out of the upper rail.
Before installation, slide the piston into the cylinder. Install the channels in reverse order.
Assembling the 10 - 300 μL multi-channel pipette
1.
2.
3.
4.
Fit the cover plate with the integrated ejector.
Push the latches upwards.
To mount, push the lower part into the upper part until it audibly engages.
Carry out a gravimetric test of the systematic and random error (see p. 35).
This test ensures that no parts were confused during assembly and that the pipette has been
assembled correctly.
21
Eppendorf Research® plus — Operating manual
5 Maintenance
5
Maintenance
5.5
NOTICE!
Hint!
5.5.1
Adjusting pipettes
NOTICE! Incorrect dispensing volume for special liquids and from temperature
differences.
Solutions which differ greatly from water in terms of their physical data, or temperature
differences between the pipette, pipette tip and liquid can result in incorrect dispensing
volumes.
Avoid temperature differences between pipette, pipette tip and liquid.
Make sure that the temperature is constant, between 20 and 25°C and at ±0.5°C.
Check the dispensing volume and readjust the pipette in case of deviations.
The systematic and random errors recorded on delivery can be found in the Certificate
of Conformity. This certificate is included in the delivery. Changes to the factory
adjustment will render the certificate void.
General notes on user and factory adjustment settings
The Research plus was adjusted, tested and fitted with a gray adjustment seal with the
abbreviation "ADJ" before delivery. The adjustment display on the side reads "0".
Changing the adjustment of the Research plus is sometimes recommended for solutions which
are very different from water with regard to their density, viscosity, surface tension and/or vapor
pressure etc. If the density of an aqueous solution changes by approximately ±10%, for example
because of the salt concentration, the volume changes by approximately ±0.2%. This statement
does not apply if other relevant properties of the liquid also change.
If the pipette is used at extremely high altitudes, it must be calibrated to the ambient air pressure.
At 1 000 m above sea level, the volume error of a 100 μL pipette is about –0.3%.
When using special tips, that is, tips that significantly differ from standard tips in their geometry,
changing the adjustment can improve the dispensing accuracy (systematic error). The CD
Research plus contains adjustment tips for epT.I.P.S. long.
Adjustment changes can be reset by simple steps.
In addition to changing the user adjustment, a Research plus with variable volume setting can be
permanently changed by altering the factory adjustment (see Changing the factory adjustment
on p. 25).
Changes made to the user or factory adjustment do not affect dispensing precision (random
error). Precision can be improved by exchanging worn parts. Precision is also considerably
affected by handling.
Before changing the adjustment or factory calibration, you must check the existing dispensing
volume.
The actual volume can be checked by weighing:
22
Eppendorf Research® plus — Operating manual
5 Maintenance
5
Maintenance
The density of distilled water is approx. 0.9982 mg/μL at 20°C and 0.9965 mg/μL at 27°C.
If the set volume corresponds to the actual volume, no correction is necessary.
If there is a difference between the actual volume and the set volume of distilled water, please
check the following:
• Is there any liquid dripping from the tip?
• Is the pipette tip fitted leak-proof?
• Is the tip cone undamaged?
• Are the piston and the cylinder leak-proof?
Adequate leak tightness is ensured when no drop is formed at the pipette tip after aspiration
of the nominal volume with distilled water and a waiting time of approx. 15 s. Hold the pipette
vertically, making sure not to touch the pipette tip. Prewet the tip several times in the case of
nominal volumes ≤ 20 μL.
• Does the temperature of the pipetted liquid correspond to:
– the temperature of the device?
– the ambient air temperature?
• Is the weighing location free from drafts?
• Does the work method and pipetting speed permit complete aspiration and dispensing of the
liquid?
• Has the correct numerical value for "Density liquids at weighing temperature" been used for
the calculation of the actual volume?
• Is the set volume correct?
• For very small volumes (<10 μL): is the fine balance sufficiently sensitive (balance resolution:
0.001 mg)?
• Were original epT.I.P.S pipette tips used for testing?
Information as to which pipette tip must be used as a test tip for checking the technical data
can be found in the "Technical Data" chapter of the operating manual.
No adjustment changes are allowed unless you can answer all the questions with "yes". In all
other cases, the problems associated with the questions answered with "no" must be eliminated.
If the problem is remedied by exchanging a complete lower part or other parts that have an effect
on the volume, proper assembly must be verified by carrying out a gravimetric test. Information
on the systematic and random errors to be met can be found in the "Technical Data" chapter.
23
Eppendorf Research® plus — Operating manual
5 Maintenance
5
5.5.2
Changing the user adjustment
If the adjustment is changed, the volume changes by a certain value. Strictly speaking, the
change only applies to the testing volume.
Maintenance
Auxiliary equipment
• Supplied adjustment tool (order no. 3120 633.006)
• Supplied red adjustment seal (ADJ)
Example
You readjust a 10 -100 μL pipette with a volume setting of 100 μL by 1 μL (1 μL 1%). If the
volume setting is 10 μL, the pipette is also adjusted by 1 μL ( 10%).
1.
2.
3.
4.
Remove the gray adjustment seal.
Keep the ejector pressed.
Insert the adjustment tool (from the delivery package).
Turn the adjustment tool until the adjustment display shows the desired value.
5. Place the Research plus on a horizontal surface
(table). When completing the adjustment, look
absolutely vertically at the window and read the set
value via the backsight in the viewing window.
6. Carry out weighings to verify accuracy and precision.
7. After the tests, close the opening with the red adjustment seal (from the delivery package).
If the adjustment is meant for a specific liquid, mark the pipette accordingly. Use the labeling area
on the pipette for this purpose and write down the liquid and the volume. Carry out a gravimetric
test for each adjustment change. Follow the test procedures of EN ISO 8655-2 and 8655-6. A
SOP (Standard Operation Procedure) and further information on user and factory adjustment
settings can be found on the Research plus CD and on our website www.eppendorf.com.
24
Eppendorf Research® plus — Operating manual
5 Maintenance
5.5.3
5
Changing the factory adjustment
Maintenance
Auxiliary equipment
• Supplied safety plug tool
• Supplied pin to loosen the safety plug
It is possible to change the factory adjustment with the
corresponding accessories for a Research plus with variable
volume setting. If the factory adjustment of the Research plus has
been changed by a user, this can be recognized by a red safety
plug behind the ejector. If the Research plus has been adjusted and
calibrated by Eppendorf AG, this is indicated by a gray safety plug.
The systematic and random errors recorded on delivery can be found in a "Certificate of
Conformity". This certificate is included in the delivery. Changes to the factory adjustment will
render the certificate void.
If the dispensing accuracy is only be to changed temporarily, the correct method is to change the
user adjustment. Before changing the user or factory adjustment, observe the general notes
(see General notes on user and factory adjustment settings on p. 22) and the associated
gravimetric tests.
Hint!
5.5.4
In the case of Research plus fixed-volume pipettes, modifications are only possible by
changing the user adjustment (see Changing the user adjustment on p. 24).
Research plus variable - changing the factory adjustment
If a gravimetric test indicates that an error needs to be corrected and you are required to carry out
a change to the factory adjustment, proceed as follows:
1. Check whether the adjustment display on
the side is set to "0".
If the adjustment display is not set to "0",
you will first need to set it to "0" with the
adjustment tool. In this case, instead of
continuing with the factory adjustment
changes, carry out a gravimetric test of the
Research plus with the adjustment display
set to "0".
25
Eppendorf Research® plus — Operating manual
5 Maintenance
5
Maintenance
2. Mark the volume setting ring and the ejector
with a common mark with a pen. This mark
serves as an orientation for factory
adjustment changes. When changing the
factory adjustment, you can turn the volume
setting ring, without the volume display
changing. The mark on the volume setting
ring and the ejector informs you how far you
have moved from the factory setting.
3. Keep the ejector pressed and remove the
safety plug with the pin.
26
Eppendorf Research® plus — Operating manual
5 Maintenance
5
4. Continue to keep the ejector pressed. Insert
the safety plug tool such that the counter
locking mechanism is pushed down.
Maintenance
5. Turn the volume setting ring slightly to change the volume. Proceed as shown in the figure.
27
Eppendorf Research® plus — Operating manual
5 Maintenance
5
This results in the following approximate volume changes:
Single-channel
Maintenance
Nominal volume +½ revolution
+¼ revolution
–¼ revolution
–½ revolution
Color code
28
2.5 μL
dark gray
0.106 μl
0.053 μl
–0.053 μL
–0.106 μL
10 μL
medium gray
0.53 μl
0.27 μl
–0.27 μL
–0.53 μL
20 μL
light gray
1.06 μl
0.53 μl
–0.53 μL
–1.06 μL
20 μL
yellow
1.07 μl
0.54 μl
–0.54 μL
–1.07 μL
100 μL
yellow
5.4 μl
2.7 μl
–2.7 μL
–5.4 μL
200 μL
yellow
10.8 μl
5.4 μl
–5.4 μL
–10.8 μL
300 μL
orange
10.7 μl
5.4 μl
–5.4 μL
–10.7 μL
1 000 μL
blue
54 μL
27 μL
–27 μL
–54 μL
5 mL
purple
271 μL
135 μL
–135 μL
–271 μL
10 mL
turquoise
542 μL
271 μL
–271 μL
–542 μL
Eppendorf Research® plus — Operating manual
5 Maintenance
5
Multi-channel
Nominal volume +½ revolution
+¼ revolution
–¼ revolution
–½ revolution
10 μL
medium gray
0.53 μl
0.27 μl
–0.27 μL
–0.53 μL
100 μL
yellow
5.4 μl
2.7 μl
–2.7 μL
–5.4 μL
300 μL
orange
10.7 μl
5.4 μl
–5.4 μL
–10.7 μL
Maintenance
Color code
The values mentioned are theoretical values and are for orientation purposes only. The
volume changes mentioned apply to each volume setting. For the volume change, you
should first set the optimal value for 10% of the nominal volume and then carry out a
gravimetric test. Afterwards carry out gravimetric tests to check 50% and 100% of the
nominal volume with this setting. Change the selected setting again if necessary to achieve
the best possible correction for all volumes. Use the error limits in accordance with ISO
8655-2 (see p. 31) and the technical data provided by Eppendorf AG (see p. 35) to decide
whether the data obtained meets your requirements.
6. Slide the locking mechanism up and carry
out a gravimetric test of the changes which
have been made.
7. If the measured gravimetric values meet your requirements: Slide the locking mechanism up.
29
Eppendorf Research® plus — Operating manual
5 Maintenance
5
Maintenance
8. Insert the red safety plug on the tool into the
opening of the Research plus and break it
off from the tool.
The red safety plug on the pipette indicates
that the Research plus has been adjusted
by the user. If the adjustment display had
also been set to "0" before, you must close
the opening with a new, red adjustment seal
at the position for the adjustment seal.
9. Document the changes made and the measurements conducted. Remove the mark on the
volume setting ring and the ejector. The red safety plug on the pipette indicates that the
Research plus has been adjusted and calibrated by the user.
30
Eppendorf Research® plus — Operating manual
5 Maintenance
5.5.5
5
Error limits in accordance with ISO 8655-2
Error limits in accordance with ISO 8655-2 - Research plus variable single-channel
Test tip
epT.I.P.S. Color
code
Testing
volume
0.5 - 10 μL
Increment:
0.01 μL
2 - 20 μL
Increment:
0.02 μL
2 - 20 μL
Increment:
0.02 μL
10 - 100 μL
Increment:
0.01 μL
20 - 200 μL
Increment:
0.02 μL
medium gray
0.1 - 20 μL
40 mm
light gray
0.5 - 20 μL L
46 mm
yellow
2 - 200 μL
53 mm
yellow
2 - 200 μL
53 mm
yellow
2 - 200 μL
53 mm
Random
±%
± μL
±%
± μL
0.1 μL
± 100.0
± 0.1
± 75.0
± 0.075
0.25 μL
± 50.0
± 0.125
± 30.0
± 0.075
1.25 μL
± 10.0
± 0.125
± 6.0
± 0.075
2.5 μL
± 5.0
± 0.125
± 3.0
± 0.075
0.5 μL
± 24.0
± 0.12
± 16.0
± 0.08
1 μL
± 12.0
± 0.12
± 8.0
± 0.08
5 μL
± 2.4
± 0.12
± 1.6
± 0.08
10 μL
± 1.2
± 0.12
± 0.8
± 0.08
2 μL
± 10.0
± 0.2
± 5.0
± 0.1
10 μL
± 2.0
± 0.2
± 1.0
± 0.1
20 μL
± 1.0
± 0.2
± 0.5
± 0.1
2 μL
± 10.0
± 0.2
± 5.0
± 0.1
10 μL
± 2.0
± 0.2
± 1.0
± 0.1
20 μL
± 1.0
± 0.2
± 0.5
± 0.1
10 μL
± 8.0
± 0.8
± 3.0
± 0.3
50 μL
± 2.0
± 0.8
± 0.6
± 0.3
100 μL
± 0.8
± 0.8
± 0.3
± 0.3
20 μL
± 8.0
± 1.6
± 3.0
± 0.6
100 μL
± 2.0
± 1.6
± 0.6
± 0.6
200 μL
± 0.8
± 1.6
± 0.3
± 0.6
Length
dark gray
0.1 - 10 μL
34 mm
Error
Systematic
Volume range
0.1 - 2.5 μL
Increment:
0.002 μL
Error limits
Maintenance
Model
31
Eppendorf Research® plus — Operating manual
5 Maintenance
5
Error limits in accordance with ISO 8655-2 - Research plus variable single-channel
Model
Test tip
Maintenance
epT.I.P.S. Color
code
Testing
volume
100 - 1 000 μL
Increment:
1 μL
0.5 - 5 mL
Increment:
5 μL
1 - 10 mL
Increment:
10 μL
32
blue
50 - 1 000 μL
71 mm
purple
0.1 - 5 mL
120 mm
turquoise
1 – 10 ml
165 mm
Random
±%
± μL
±%
± μL
30 μL
± 13.0
± 3.9
± 5.0
± 1.5
150 μL
± 3.0
± 3.9
± 1.0
± 1.5
300 μL
± 1.3
± 3.9
± 0.5
± 1.5
100 μL
± 8.0
± 8.0
± 3.0
± 3.0
500 μL
± 2.0
± 8.0
± 0.6
± 3.0
1 000 μL
± 0.8
± 8.0
± 0.3
± 3.0
0.5 mL
± 8.0
± 40.0
± 3.0
± 15.0
2.5 mL
± 1.6
± 40.0
± 0.6
± 15.0
5.0 mL
± 0.8
± 40.0
± 0.3
± 15.0
1.0 mL
± 6.0
± 60.0
± 3.0
± 30.0
5.0 mL
± 1.2
± 60.0
± 0.6
± 30.0
10.0 mL
± 0.6
± 60.0
± 0.3
± 30.0
Length
orange
20 - 300 μL
55 mm
Error
Systematic
Volume range
30 - 300 μL
Increment:
0.02 μL
Error limits
Eppendorf Research® plus — Operating manual
5 Maintenance
5
Error limits in accordance with ISO 8655-2 - Research plus variable multi-channel
Model
Test tip
10 - 100 μL
Increment:
0.01 μL
30 - 300 μL
Increment:
0.02 μL
yellow
2 - 200 μL
53 mm
orange
20 - 300 μL
55 mm
Random
±%
± μL
±%
± μL
0.5 μL
± 48.0
± 0.24
± 32.0
± 0.16
1 μL
± 24.0
± 0.24
± 16.0
± 0.16
5 μL
± 4.8
± 0.24
± 3.2
± 0.16
10 μL
± 2.4
± 0.24
± 1.6
± 0.16
10 μL
± 16.0
± 1.6
± 6.0
± 0.6
50 μL
± 3.2
± 1.6
± 1.2
± 0.6
100 μL
± 1.6
± 1.6
± 0.6
± 0.6
30 μL
± 26.0
± 7.8
± 10.0
± 3.0
150 μL
± 5.2
± 7.8
± 2.0
± 3.0
300 μL
± 2.6
± 7.8
± 1.0
± 3.0
Length
medium gray
0.1 - 20 μL
40 mm
Error
Systematic
Volume range
0.5 - 10 μL
Increment:
0.01 μL
Error limits
Maintenance
epT.I.P.S. Color
code
Testing
volume
33
Eppendorf Research® plus — Operating manual
5 Maintenance
5
5.6
Decontamination before shipment
Maintenance
If you wish to return the pipette to Eppendorf AG or an Eppendorf AG service partner to be
checked or repaired, please note the following:
CAUTION!
CAUTION! Use of a contaminated device may result in personal injuries and
damage to the device.
Clean and decontaminate the Research plus before shipping or storage according
to the cleaning instructions.
Read the instructions regarding the decontamination certificate included on the
Research plus CD or visit (www.eppendorf.com/decontamination).
Enter the serial number of the Research plus in the decontamination certificate.
This can be found at the lower end of the entry field.
Enclose the fully-completed decontamination certificate for returned goods with the
Research plus.
Hazardous substances are:
• solutions presenting a hazard to health
• potentially infectious agents
• organic solvents and reagents
• radioactive substances
• proteins presenting a hazard to health
• DNA
1. Please note the information in the document "Decontamination certificate for product
returns". You can find it as a PDF file on our homepage www.eppendorf.com.
2. Enter the serial number of the Research plus in the decontamination certificate.
This can be found at the lower end of the entry field.
3. With the shipment please include the completed and signed "Decontamination certificate for
product returns" for each pipette.
34
Eppendorf Research® plus — Operating manual
6 Technical data
6
6.1
Technical data
Research plus single-channel variable
Research plus variable single-channel
Model
Test tip
epT.I.P.S. Color
code
Testing
volume
± μL
±%
± μL
0.1 μL
± 48
± 0.048
± 12
± 0.012
0.25 μL
± 12
± 0.03
±6
± 0.015
1.25 μL
± 2.5
± 0.031
± 1.5
± 0.019
2.5 μL
± 1.4
± 0.035
± 0.7
± 0.018
2 - 20 μL
Increment:
0.02 μL
2 - 20 μL
Increment:
0.02 μL
10 - 100 μL
Increment:
0.1 μL
20 - 200 μL
Increment:
0.2 μL
medium gray
0.1 - 20 μL
40 mm
0.5 μL
±8
± 0.04
±5
± 0.025
1 μL
± 2.5
± 0.025
± 1.8
± 0.018
5 μL
± 1.5
± 0.075
± 0.8
± 0.04
10 μL
± 1.0
± 0.1
± 0.4
± 0.04
light gray
0.5 - 20 μL L
46 mm
2 μL
±5
± 0.1
± 1.5
± 0.03
10 μL
± 1.2
± 0.12
± 0.6
± 0.06
20 μL
± 1.0
± 0.2
± 0.3
± 0.06
yellow
2 - 200 μL
53 mm
2 μL
±5
± 0.1
± 1.5
± 0.03
10 μL
± 1.2
± 0.12
± 0.6
± 0.06
20 μL
± 1.0
± 0.2
± 0.3
± 0.06
yellow
2 - 200 μL
53 mm
10 μL
±3
± 0.3
±1
± 0.1
50 μL
±1
± 0.5
± 0.3
± 0.15
100 μL
± 0.8
± 0.8
± 0.2
± 0.2
yellow
2 - 200 μL
53 mm
20 μL
± 2.5
± 0.5
± 0.7
± 0.14
100 μL
±1
±1
± 0.3
± 0.3
200 μL
± 0.6
± 1.2
± 0.2
± 0.4
Technical data
0.5 - 10 μL
Increment:
0.01 μL
Random
±%
Length
dark gray
0.1 - 10 μL
34 mm
6
Error
Systematic
Volume range
0.1 - 2.5 μL
Increment:
0.002 μL
Error limits
35
Eppendorf Research® plus — Operating manual
6 Technical data
Research plus variable single-channel
Model
Test tip
epT.I.P.S. Color
code
6
Testing
volume
Technical data
100 - 1 000 μL
Increment:
1 μL
0.5 - 5 mL
Increment:
0.005 mL
1 - 10 mL
Increment:
0.01 mL
36
blue
50 - 1 000 μL
71 mm
purple
0.1 - 5 mL
120 mm
turquoise
1 – 10 ml
165 mm
Random
±%
± μL
±%
± μL
30 μL
± 2.5
± 0.75
± 0.7
± 0.21
150 μL
±1
± 1.5
± 0.3
± 0.45
300 μL
± 0.6
± 1.8
± 0.2
± 0.6
100 μL
±3
±3
± 0.6
± 0.6
500 μL
±1
±5
± 0.2
±1
1 000 μL
± 0.6
±6
± 0.2
±2
0.5 mL
± 2.4
± 12
± 0.6
±3
2.5 mL
± 1.2
± 30
± 0.25
±6
5.0 mL
± 0.6
± 30
± 0.15
±8
1.0 mL
±3
± 30
± 0.6
±6
5.0 mL
± 0.8
± 40
± 0.2
± 10
10.0 mL
± 0.6
± 60
± 0.15
± 15
Length
orange
20 - 300 μL
55 mm
Error
Systematic
Volume range
30 - 300 μL
Increment:
0.2 μL
Error limits
Eppendorf Research® plus — Operating manual
6 Technical data
6.2
Research plus multi-channel variable
Research plus variable multi-channel
Model
Test tip
epT.I.P.S. Color
code
Testing
volume
± μL
±%
± μL
0.5 μL
± 12
± 0.06
± 8.0
± 0.04
1 μL
± 8.0
± 0.08
± 5.0
± 0.05
5 μL
± 4.0
± 0.2
± 2.0
± 0.1
10 μL
± 2.0
± 0.2
± 1.0
± 0.1
30 – 300 μL
Increment:
0.2 μL
yellow
2 - 200 μL
53 mm
10 μL
± 3.0
± 0.3
± 2.0
± 0.2
50 μL
± 1.0
± 0.5
± 0.8
± 0.4
100 μL
± 0.8
± 0.8
± 0.3
± 0.3
orange
20 - 300 μL
55 mm
30 μL
± 3.0
± 0.9
± 1.0
± 0.3
150 μL
± 1.0
± 1.5
± 0.5
± 0.75
300 μL
± 0.6
± 1.8
± 0.3
± 0.9
Technical data
10 – 100 μL
Increment:
0.1 μL
Random
±%
Length
medium gray
0.1 - 20 μL
40 mm
6
Error
Systematic
Volume range
0.5 – 10 μL
Increment:
0.01 μL
Error limits
37
Eppendorf Research® plus — Operating manual
6 Technical data
6.3
Research plus fix
Research plus fixed volume
Model
6
Test tip
Error limits
epT.I.P.S. Color
code
Technical data
Volume range
Length
Error
Systematic
Random
±%
± μL
±%
± μL
10 μL
medium gray
0.1 - 20 μL
40 mm
± 1.2
± 0.12
± 0.6
± 0.06
20 μL
light gray
0.5 - 20 μL L
46 mm
± 0.8
± 0.16
± 0.3
± 0.06
10 μL
yellow
2 - 200 μL
53 mm
± 1.2
± 0.12
± 0.6
± 0.06
± 1.0
± 0.2
± 0.3
± 0.06
25 μL
± 1.0
± 0.25
± 0.3
± 0.08
50 μL
± 0.7
± 0.35
± 0.3
± 0.15
100 μL
± 0.6
± 0.6
± 0.2
± 0.2
200 μL
± 0.6
± 1.2
± 0.2
± 0.4
± 0.6
± 1.2
± 0.2
± 0.4
± 0.6
± 1.5
± 0.2
± 0.5
500 μL
± 0.6
±3
± 0.2
±1
1 000 μL
± 0.6
±6
± 0.2
±2
20 μL
200 μL
250 μL
blue
50 - 1 000 μL
71 mm
Test conditions according to ISO 8655-1, 8655-2 and 8655-6 for piston-stroke pipettes with air
cushion by means of a fine balance with evaporation protection, tested by the German gauging
office.
Number of determinations: 10; water according to ISO 3696; 20°C to 25°C ±0.5°C constant;
with pre-wetted pipette tip; dispensing against the tube wall.
38
Eppendorf Research® plus — Operating manual
6 Technical data
6.4
Ambient conditions
Temperature range
Relative humidity
Storage without transport packaging
–5 to 45°C
10 to 95%
Operating conditions
5 to 40°C
10 to 95%
6
Technical data
Technical specifications subject to change.
39
Eppendorf Research® plus — Operating manual
7 Ordering information
7
Ordering information
The ordering information can be found on the Research plus CD.
Hint!
Ordering information
7
40
Eppendorf offices
Eppendorf offices
AUSTRALIA & NEW ZEALAND
Eppendorf South Pacific Pty. Ltd.
Phone: +61 2 9889 5000
Fax: +61 2 9889 5111
E-mail: [email protected]
Internet: www.eppendorf.com.au
CANADA
Eppendorf Canada Ltd.
Phone: +1 905 826 5525
Fax: +1 905 826 5424
E-mail: [email protected]
Internet: www.eppendorfna.com
FRANCE
Eppendorf France S.A.R.L.
Phone: +33 1 30 15 67 40
Fax: +33 1 30 15 67 45
E-mail: [email protected]
Internet: www.eppendorf.fr
ITALY
Eppendorf s.r.l.
Phone: +390 2 55 404 1
Fax: +390 2 58 013 438
E-mail: [email protected]
Internet: www.eppendorf.it
SOUTH & SOUTHEAST ASIA
Eppendorf Asia Pacific Sdn. Bhd.
Phone: +60 3 8023 2769
Fax: +60 3 8023 3720
E-mail:
[email protected]
Internet: www.eppendorf.com.my
UNITED KINGDOM
Eppendorf UK Limited
Phone: +44 1223 200 440
Fax: +44 1223 200 441
E-mail: [email protected]
Internet: www.eppendorf.co.uk
AUSTRIA
Eppendorf Austria GmbH
Phone: +43 (0) 1 890 13 64 - 0
Fax: +43 (0) 1 890 13 64 - 20
E-mail: [email protected]
Internet: www.eppendorf.at
CHINA
Eppendorf China Ltd.
Phone: +86 21 38560500
Fax: +86 21 38560555
E-mail: [email protected]
Internet: www.eppendorf.cn
GERMANY
Eppendorf Vertrieb
Deutschland GmbH
Phone: +49 2232 418-0
Fax: +49 2232 418-155
E-mail: [email protected]
Internet: www.eppendorf.de
JAPAN
Eppendorf Co. Ltd.
Phone: +81 3 5825 2363
Fax: +81 3 5825 2365
E-mail: [email protected]
Internet: www.eppendorf.jp
SPAIN
Eppendorf Ibérica S.L.U.
Phone: +34 91 651 76 94
Fax: +34 91 651 81 44
E-mail: [email protected]
Internet: www.eppendorf.es
USA
Eppendorf North America, Inc.
Phone: +1 516 334 7500
Fax: +1 516 334 7506
E-mail: [email protected]
Internet: www.eppendorfna.com
BRAZIL
Eppendorf do Brasil Ltda.
Phone: +55 11 30 95 93 44
Fax: +55 11 30 95 93 40
E-mail:
[email protected]
Internet: www.eppendorf.com.br
CZECH REP. & SLOVAKIA
Eppendorf Czech & Slovakia s.r.o.
Phone: +420 323 605 454
Fax: +420 323 605 454
E-mail: [email protected]
Internet: www.eppendorf.cz /
www.eppendorf.sk
INDIA
Eppendorf India Limited
Phone: +91 44 42 11 13 14
Fax: +91 44 42 18 74 05
E-mail: [email protected]
Internet: www.eppendorf.co.in
NORDIC
Eppendorf Nordic Aps
Phone: +45 70 22 2970
Fax: +45 45 76 7370
E-mail: [email protected]
Internet: www.eppendorf.dk
SWITZERLAND
Vaudaux-Eppendorf AG
Phone: +41 61 482 1414
Fax: +41 61 482 1419
E-mail: [email protected]
Internet: www.eppendorf.ch
OTHER COUNTRIES
Internet: www.eppendorf.com/
worldwide
Your local distributor: www.eppendorf.com/worldwide
Eppendorf AG · 22331 Hamburg · Germany · Tel: +49 40 538 01-0 · Fax: +49 40 538 01-556
E-Mail: [email protected]
Eppendorf North America, Inc. · 102 Motor Parkway, Suite 410 · Hauppauge, NY 11788-5178 · USA
Tel: +1 516 334 7500 · Toll free phone: +1 800 645 3050 · Fax: +1 516 334 7506 · E-Mail: [email protected]
Application Support
Europe, International: Tel: +49 1803 666 789 · E-Mail: [email protected]
North America: Tel: +1 800 645 3050 ext. 2258 · E-Mail: [email protected]
Asia Pacific: Tel: +60 3 8023 6869 · E-Mail: [email protected]
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