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Leica DM750
Manual
Chapter overview
Safety regulations
The Leica DM 750
Get Ready!
Get Set!
Go!
Care of the Microscope
Dimensions
42
44
47
18
28
4
15
Leica DM 750 Manual 2
Contents
Safety regulations
Symbols used in this operating manual
Health risks and dangers of use
Information for the person responsible for the instrument
Accessories, maintenance and repair
Electrical data and ambient conditions
The Leica DM 750
15
4
Get Ready!
Leica EZ Viewing Tube – integrated eyepieces
Leica Standard Viewing Tube – separated eyepieces
Installing the Substage Condenser
Get Set!
Prepare to View a Specimen Slide
18
28
Go!
Care of the Microscope
Dimensions
Leica DM 750 Manual
42
44
47
3
Safety regulations
Leica DM 750 Manual 4
Safety concept
The individual modules of the Leica DM microscopy series include an interactive CD-ROM with all relevant user manuals several languages.
Keep it in a safe place, and readily accessible to the user. User manuals and updates are also available for you to download and print from our web site at www.leica-microsystems.com.
This operating manual describes the special functions of the individual modules of the Leica
DM microscopy series and contains important instructions for their operational safety, maintenance, and accessories.
The "Safety concept" booklet contains additional safety information regarding the service work, requirements and the handling of microscope, accessories and electrical accessories as well as general safety instructions.
You can combine individual system articles with articles from external suppliers. Please read the user manual and the safety requirements of the supplier.
Before installing, operating or using the instruments, read the user manuals listed above. In particular, please observe all safety instructions.
To maintain the unit in its original condition and to ensure safe operation, the user must follow the instructions and warnings contained in these user manuals.
Leica DM 750 Manual 5
Symbols used in this operating manual
Warning of a danger
•
This symbol indicates especially important information that must be read and complied with.
Danger due to hot surface.
This symbol warns against touching accessible hot surfaces, e.g. those of light bulbs.
Failure to comply can cause the following:
Ϙ
Hazards to personnel
Ϙ
Functional disturbances or damaged instruments
Important information
This symbol indicates additional information or explanations that intend to provide clarity.
Warning of hazardous electrical voltage
This symbol indicates especially important information that is mandatory to read and observe.
Explanatory notes
ϐ
This symbol within the text points to additional information and explanations.
Failure to comply can cause the following:
Ϙ
Hazards to personnel
Ϙ
Functional disturbances or damaged instruments
Leica DM 750 Manual 6
Important Notes
Description
The Leica DM 750 microscope meets today's state of the art of technology. Nevertheless, hazards may still arise during operation. The potential risks are described below.
•
Before installing, operating or using the instruments, it is required to read this user manual. In particular, please observe all safety instructions.
Contact address
Leica Microsystems (Switzerland) Ltd.
Industry Division
Max Schmidheiny Strasse 201
CH-9435 Heerbrugg (Switzerland)
User Manual
This user manual includes important instructions related to operating safety, maintenance and accessories,
Your Leica DM 750 microscope comes with an interactive CD-ROM with all relevant user manuals. Keep it in a safe place, and readily accessible to the user. User manuals and updates are also available for you to download and print from our web site at www.leica-microsystems.com
Accessories from third-party suppliers
You can combine individual system articles with articles from external suppliers. Please read the user manual and the safety requirements of the supplier.
Original condition
To maintain the unit in its original condition and to ensure safe operation, the user must follow the instructions and warnings contained in these user manuals.
Legal requirements
Adhere to general and local regulations relating to accident prevention and environmental protection.
EC Declaration of Conformity
Electrically operated accessories are constructed based on the state of the art of technology and are provided with an EC Declaration of Conformity.
Leica DM 750 Manual 7
Instructions on use
Intended use
The Leica DM 750 microscope is an optical instrument which uses magnification and illumination to improve the visibility of objects. It is used for observing and documenting.
The Leica DM 750 microscope may be used only in closed rooms and must be placed on a solid floor.
The Leica DM 750 microscope can be used in clean rooms without any problems.
•
Position the Leica DM 750 microscope so that it can be disconnected from the power supply at any time. The power cord must remain accessible at all times, because the power supply is foreseen as mains disconnecting device.
Place of use
Only use the instruments in closed, dust free rooms and between +10°C and +40°C. Protect the devices from oil, chemicals and extreme humidity. If using the devices outdoors, protect them from dust and moisture. Never use electrical devices outdoors. Install electrical devices at least 10 cm from the wall and away from flammable substances.
Avoid large temperature fluctuations, direct sunlight and vibrations. These conditions can distort measurements and micrographic images.
In warm and warm-damp climatic zones, the individual components require special care in order to prevent the build-up of fungus.
Non-intended use
•
Using the instrument in any way contrary to the specifications in the user manual can lead to bodily harm and damage to objects. Doing so can impair the functions of the protective measures provided.
Never install any other plug (NEMA
5-15P) or unscrew any mechanical components unless expressly instructed to do so in the instructions.
The devices and accessories described in this operating manual have been tested for safety and potential hazards.
Leica DM 750 Manual 8
Instructions on use (continued)
•
The responsible Leica affiliate must be consulted whenever the instrument is altered, modified or used in conjunction with non-Leica components that are outside of the scope of this manual.
•
Unauthorized alterations to the device or noncompliant use shall void all rights to any warranty claims.
Transport
If at all possible, use the original packaging for shipping or transporting individual modules.
In order to prevent damage from vibrations, disassemble all moving parts that (according to the user manual) can be assembled and disassembled by the customer and pack them separately.
Disposal
Disposal must comply with locally applicable laws and regulations.
Integration in third-party products
When installing Leica products into third-party products, the manufacturer of the complete system or its dealer is responsible for following all applicable safety instructions, laws and guidelines.
Leica DM 750 Manual 9
Health risks and dangers of use
Health risks
Workplaces with microscopes facilitate and improve the viewing task, but they also impose high demands on the eyes and holding muscles of the user. Depending on the duration of uninterrupted work, asthenopia and musculoskeletal problems may occur. For this reason, appropriate measures for reduction of the workload must be taken:
Ϙ
Optimum workplace layout
Ϙ
Frequent changes of activity
Ϙ
Thorough training of the personnel, giving consideration to ergonomic and organizational aspects
The ergonomic design and construction of the
Leica microscopy series are intended to reduce the exertion of the user to a minimum.
Danger of infection
•
Direct contact with eyepieces is a potential transmission method for bacterial and viral infections of the eye.
Dangers during use
Ϙ
The Leica DM 750 microscope may only be connected to a grounded socket.
Ϙ
The Leica DM 750 microscope may not be operated unless it is in proper functioning condition.
The risk can be kept to a minimum by using personal eyepieces for each individual or detachable eyecups.
LED Klasse 1 /LED 1 Laser Product
The illumination meets laser class 1 requirements in accordance with IEC 60825-1 "Safety of LED products".
Class 1
LED Product
LED class 1
Leica DM 750 Manual 10
Information for the person responsible for the instrument
Information for the person responsible for the instrument
Ϙ
Ensure that the Leica DM 750 microscope is used only by qualified personnel.
Ϙ
Ensure that this user manual is always available at the Leica DM 750 microscope.
Ϙ
Carry out regular inspections to make certain that the authorized users are adhering to safety requirements.
Ϙ
When instructing new users, do so thoroughly and explain the meanings of the warning signs and messages.
Ϙ
Assign individual responsibilities for starting, operating and servicing the instrument and monitor the observance of these responsibilities.
Ϙ
Do not use the Leica DM 750 microscope unless it is in perfect condition.
Ϙ
Inform your Leica representative or Leica
Microsystems (Schweiz) AG, Industry
Division, 9435 Heerbrugg, Switzerland, immediately of any product defect that could potentially cause injury or harm.
Ϙ
If you use accessories made by third-party manufacturers with the Leica DM 750 microscope, be sure that each such manufacturer confirms the safety-engineering, harmless usability of the product and observe the product's user manual.
Ϙ
Modifications and maintenance of the Leica
DM 750 microscope may only be performed by professionals expressly authorized by
Leica.
Ϙ
Only original Leica replacement parts may be used in servicing the product.
Ϙ
After service work or technical modifications, the unit must be reconfigured with observance to our technical requirements.
Ϙ
If the unit is modified or serviced by unauthorized persons, is improperly maintained
(as long as maintenance was not carried out by us), or is handled improperly, Leica will not accept any liability.
Ϙ
The electric installation in the building must conform to the national standard, e.g. current-operated ground leakage protection (fault-current protection) is suggested.
Leica DM 750 Manual 11
Care instructions
General instructions
Ϙ
Protect the Leica DM 750 microscope against damp, vapors, acids, alkalis, and corrosive substances. Do not store chemicals in the vicinity.
Ϙ
Protect the Leica DM 750 microscope from oil and grease. Never grease or oil mechanical parts or sliding surfaces.
Ϙ
Follow the instructions of the disinfectant manufacturer.
Ϙ
It is advisable to enter a service agreement with Leica Service.
Cleaning coated parts and plastic parts
Ϙ
Dust and dirt particles should be removed with a soft brush or lint-free cotton cloth.
Ϙ
Remove coarse debris with a moistened disposable cloth.
Ϙ
Clinging dirt can be removed using petroleum ether or alcohol
Ϙ
Acetone, xylene or nitro-containing thinners must NOT be used.
Ϙ
Never use chemicals to clean colored surfaces or accessories with rubberized parts. This could damage the surfaces, and specimens could be contaminated by abraded particles.
Cleaning glass surfaces
Ϙ
Remove dust using a fine, dry and greasefree brush made from hair, by blowing with a squeeze blower, or by using a vacuum.
Ϙ
Remove dirt using a clean cloth that has been moistened with distilled water.
Ϙ
Remove tough dirt using alcohol, chloroform or petroleum ether.
Leica DM 750 Manual 12
Accessories, maintenance and repair
Accessories
Only the following accessories may be used with the Leica DM 750 microscope:
Ϙ
The Leica accessories described in this user manual.
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Other accessories, provided that these have been expressly approved by Leica as being technically safe in this context.
Maintenance
Ϙ
The Leica DM 750 microscope is basically maintenance-free. To ensure that it always operates safely and reliably, we recommend that you take the precaution of contacting the responsible service organization.
You can arrange for periodic inspections or, if appropriate, conclude a maintenance contract with them.
Repairs and service work
Ϙ
Only original Leica Microsystems spare parts may be used.
Ϙ
Before opening the instruments, switch off the power and unplug the power cable.
Ϙ
Avoid contact with powered electrical circuits, which can lead to injury.
Ϙ
It is advisable to enter a service agreement with Leica Service.
Ϙ
For maintenance and repair, only OEM spare parts may be used.
Service address
In case of problems, please contact us as follows:
Leica Microsystems (Switzerland) Ltd.
Industry Division
Max Schmidheiny Strasse 201
CH-9435 Heerbrugg (Switzerland)
Phone +41 71 726 33 28
Leica DM 750 Manual 13
Electrical data and ambient conditions
Fuse Replacement
Unplug the instrument before changing any fuses. The
Leica DM 750 has two fuses, which are located behind the power cord receptacle.
•
Use only fuse type: 5×20 mm, 3.15 Amp/250V, Quick acting (# 13RFAG300002)
Electrical data
Input: 100-240 V, 50/60 Hz, 3 W
Camera module, USB or external power supply
Input: 100-240 VAC, 50/60Hz, 0.5A
Output: 5 VDC (SELV), 2.4 A, 12 W max.
Environment
Temperature for use
Storage temperature
Manipulation shock
Transport shock (unpacked)
+10 °C … +40 °C
-20 °C … +52 °C
+50 °F … +104 °F
25 mm on 50 mm hard wood
100 g / 6 ms
Transport shock (packed)
Transport vibrations (unpacked)
800 mm free fall
5–200 Hz / 1.5 g
Atmospheric pressure "in use" and for storage: 500–1060 mBar
Humidity "in use" and for storage: 20–90%
Installation Category II (Overvoltage Category)
Pollution degree 2
Leica DM 750 Manual 14
The Leica DM 750
Leica DM 750 Manual 15
Introduction
Thank you for purchasing the Leica DM 750
Compound Microscope from Leica Microsystems. This model’s exclusive design features and full range of accessories make it a truly versatile, high quality-instrument for educational and routine clinical applications.
•
This instrument should only be used as described. Hazards may result if used improperly.
Leica DM 750 Manual 16
Unpacking
Ϙ
Carefully remove the microscope and any components from the packing carton.
Ϙ
Check the components against the planned configuration.
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Optional items such as contrast accessories, camera adapters, cameras, and carrying cases are not shipped as part of the standard equipment. These items are delivered in separate packages.
Ϙ
Please do not discard any of the packing materials. They should be used for safely storing and transporting the instrument should the need arise.
Leica DM 750 Manual 17
Get Ready!
Leica DM 750 Manual 18
Substage Illumination
Substage Illumination
The Leica DM 750 is available with two types of Substage Illumination. Identify which type of Illumination you have, as this will be important to know later.
Type 1: Standard Illumination
Adjustable condenser centration with provided tool.
Type 2: Koehler Illumination
Adjustable condenser centration with thumbscrews and adjustable Koehler field diaphragm.
Leica DM 750 Manual 19
Attaching the viewing Tubes
Tools used
Ϙ
Allen key
There are two types of viewing tube.
Identify which viewing tube you have:
1. Slightly unscrew the set screw (located on top of the stand) with the Allen key tool provided.
Leica DM 750
Type 1: Leica EZ viewing tube with integrated eyepieces.
2. Insert the dovetail into the stand support and tighten the set screw firmly without forcing it. This precisely positions the viewing tube onto the optical axis of the microscope, regardless of the selected rotation.
Type 2: Standard viewing tube with separate eyepieces
Manual 20
Leica EZ Viewing Tube – integrated eyepieces
1. To use the captive thumbscrew, completely remove the set screw that comes with the stand.
2. Position the Leica EZ viewing tube back in place on the stand.
Leica EZ viewing tube with integrated eyepieces.
To rotate the Leica EZ viewing tube, either loosen the set screw on the stand or replace the set screw with the (optional) captive thumbscrew.
•
Be sure the thumbscrew portion of the captive thumbscrew is fully loosened before attaching it to the stand:
3. Attach the captive thumbscrew using the wrench tool that comes with the captive thumbscrew.
Leica DM 750 Manual 21
Leica EZ Viewing Tube – integrated eyepieces (cont.)
You can now safely rotate the Leica EZ viewing tube by loosening the captive thumbscrew, rotating the viewing tube, and tightening the captive thumbscrew again.
The eyepieces are already integrated with the Leica EZ viewing tube and are pre-adjusted for corrected vision; therefore no eyepiece adjustment or insertion is necessary.
Go on to the section "Eyeguards" on page 24.
Leica DM 750 Manual 22
Leica Standard Viewing Tube – separated eyepieces
1. Insert the eyepieces into the eyetubes.
2. Lock the eyepieces into the eyetubes by tightening the silver screws on the underside of the eyetubes with a standard Cross type (Phillips) screwdriver (not provided).
Standard viewing tube; eyetubes do not yet contain the eyepieces
The standard viewing tube has a rotatable dovetail. Therefore, you can now rotate the standard viewing tube freely in any orientation.
The eyepieces will still rotate, but they will be captive in the eyetubes.
Leica DM 750 Manual 23
Eyeguards
If you wear eyeglasses for microscope viewing, keep the rubber eyeguards folded down. If you do not wear eyeglasses, you may find it useful to unfold the rubber eyeguards in order to help block out ambient room light.
If you have purchased a standard microscope configuration, you will notice that the objectives are already installed on the nosepiece and the substage condenser is already installed on the stand. In this case, go to section
"Operation" on page 29. If you purchased your
Leica DM 750 by components and not by standard configuration, please continue to section
"Installing Objectives" on page 25.
Leica DM 750 Manual 24
Installing Objectives
Installing Objectives
•
Always rotate the nosepiece by using the knurled nosepiece ring.
As you rotate the nosepiece clockwise, attach the objectives by screwing them into the nosepiece holes starting with the lowest magnification and advancing to the highest magnification.
Leica DM 750 Manual 25
Installing the Substage Condenser
Substage Condenser
The Leica DM 750 has an open substage condenser mount, therefore the condenser needs to be installed.
1. Raise the stage to its highest position using the coarse focus knob on the side of the microscope.
3. Completely loosen the two set screws (or thumbscrews if you have a Koehler stand) on the condenser mount.
The substage condenser mount is open
2. Adjust the condenser mount to its lowest position using the condenser focus knob, located on the left-hand side of the substage.
Leica DM 750 Manual 26
Installing the Substage Condenser (cont.)
4. Slide the substage condenser into the condenser mount underneath the stage by aligning the positioning pin on the underside of the condenser into the slot on the back of the fork.
5. Raise the condenser to its highest position using the condenser focus knob, located on the left-hand side of the substage.
Leica DM 750
6. Equally tighten the two set screws with the supplied tool (or tighten the thumbscrews if you have a Koehler stand) so that the top lens of the condenser is centered beneath the objective in the operating position to roughly center the substage condenser.
You will make a more accurate condenser centration when you come to section
"Complete Condenser Centration" on page 30.
Manual 27
Get Set!
Leica DM 750 Manual 28
Turning on the microscope
Work Surface
•
Always use your microscope on a hard, stable surface.
Power Cord
•
If the power cord is not already attached, attach it securely to the back of the microscope.
Setting the Illumination Intensity
•
Set the illumination control knob
(located on the bottom left-hand of the microscope stand) to the lowest setting to start. The illumination control knob allows you to adjust the intensity of light produced by the illumination system.
Plug in and Turn on the Microscope
1. Plug the microscope power cord into a suitable grounded electrical outlet. A grounded
3-wire cord is provided.
2. Turn the microscope on using the switch on the bottom right-hand side of the microscope stand.
Leica DM 750 Manual 29
Complete Condenser Centration
If you have purchased a standard Leica
DM 750 configuration, the condenser has already been pre-centered by Leica Microsys-
tems. Go on to section "Condenser Aperture"
on page 43.
2. Draw an "X" on a business card size paper and place it on the light exit of the microscope stand, so the "X" is centered over the illumination.
1. Open the condenser aperture by rotating the knurled ring on the condenser to the right.
Not centered
•
Be sure the condenser is raised to its highest position
3. Look through the eyepieces and use the provided tool with the set screws (or thumbscrews if you have a Leica DM 750 with Koehler illumination) to more accurately center the condenser by observing the X and centering it in the field of view by tightening the screws.
Centered
Leica DM 750 Manual 30
Complete Condenser Centration (cont.)
4. If you have a Leica DM 750 with standard illumination (no Koehler field diaphragm), you can now lock the condenser into position with the set screws in the back of each side of the condenser fork using the tool provided.
If you have a Leica DM 750 with a Koehler field diaphragm, you will center and focus the condenser even more accurately when you
come to section "Koehler Setup" on page 37.
Set screw
Leica DM 750 Manual 31
Using the Condenser
Using the Condenser
The condenser is furnished with an iris diaphragm, which can be adjusted to match the effective numerical aperture of the objective
1. To open and close this diaphragm, simply rotate the knurled condenser ring to the right or to the left so that the line on the rotating ring aligns with the objective magnification in use.
2. To start, fully open the iris diaphragm of the aperture condenser by rotating the condenser ring to the extreme right.
Leica DM 750
Match the line of the rotating ring with the
objective magnification in use.
Manual 32
Prepare to View a Specimen Slide
1. Place a specimen slide on the stage by sliding the specimen slide forward into the slide grips.
2. Use the X/Y stage control to position the slide so a portion of the specimen is under the objective in use.
Slide grips hold the slide in place.
Slide grips
Leica DM 750 Manual 33
Focusing
1. Rotate the nosepiece (using the knurled nosepiece ring) to move the lowest magnification objective into working position.
2. Raise the stage by rotating the coarse focus adjustment knob to its positive stop at the highest position.
3. Look into the eyepieces and adjust the illumination intensity so that it is comfortable for viewing.
The Leica DM 750 microscope stand has been factory-adjusted so that the stage focus can be found within 1.5 rotations of the fine focus adjustment from this position.
4. Using the fine focus adjustment knob, bring the specimen into sharp focus
Coarse focus adjustment knob
Leica DM 750 Manual 34
Viewing Tube Adjustment
Adjust the eyetubes
1. Adjust the eyetubes for your interpupillary distance. Fold or unfold the eyetubes to decrease or increase the distance between the eyepieces until you see one illuminated circle.
The viewing tubes maintain a constant tube length for all interpupillary settings.
This means that a change of interpupillary distance does not affect parfocality, magnification, or calibrations that depend on magnification.
Leica EZ Viewing Tube
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If you are using a Leica EZ viewing tube, which has the eyepieces integrated with the eyetubes, there are no additional adjustments necessary. Be sure you are wearing your eyeglasses or contact lenses.
Ϙ
If you have a DM 750 with standard illumination (no Koehler Field Diaphragm) go on
to section "Oil Immersion Technique" on
page 39.
Ϙ
If you have a Leica DM 750 with a Koehler
Field Diaphragm, go on to section "Koehler
Standard Viewing Tube with Two Fixed
Eyepieces
Ϙ
If you are using a standard viewing tube with two fixed eyepieces (no focusing eyepieces), there are no additional adjustments necessary. Be sure you are wearing your eyeglasses or contact lenses.
Ϙ
Ϙ
If you have a Leica DM 750 with standard illumination (no Koehler Field Diaphragm)
go on to section "Oil Immersion Technique"
on page 39.
If you have a Leica DM 750 with a Koehler
Field Diaphragm, go on to section "Koehler
Standard Viewing Tube with One or Two
Focusing Eyepieces
If you are using a standard viewing tube with one or two focusing eyepieces, you need to make some adjustments.
1. Set the focusing eyepieces to the "0" diopter setting.
Leica DM 750 Manual 35
Viewing Tube Adjustment (cont.)
If you are comfortable wearing your corrective lenses (contact lenses or eyeglasses) for microscope viewing, leave them on and your adjustments will be minimal.
2. Focus on the specimen using the fine focus adjustment knob of the microscope while looking through only one of the eyepieces
(if you have one focusable and one nonfocusable eyepiece, look through the nonfocusable eyepiece at this time). To help focus, cover or close the other eye.
3. Now switch to look only through the other eyepiece (focusing eyepiece) with your other eye. This time, focus the specimen by using the focusing capability in the focusing eyepiece.
•
Do not raise or lower the stage to focus.
4. Grip the knurl on the focusing eyepiece with one hand and rotate the top of the eyepiece with the other hand until the specimen is in focus with this eye and this focusing eyepiece. This corrects for any vision differences between your right eye and left eye.
5. Now switch to a high magnification objective (not an oil objective) and focus the microscope while viewing with both eyes.
The higher magnifications have a shallower depth of field. Therefore, after focusing with a high magnification, you will find that when you change to lower magnifications, you only have to adjust the fine focus slightly, if at all.
Ϙ
If your DM 750 has standard illumination,
please go to section "Oil Immersion Technique" on page 39.
Ϙ
If your DM 750 has Koehler setup, please
go to the next section "Koehler Setup" on
page 37.
Leica DM 750 Manual 36
Koehler Setup
If your Leica DM 750 is fitted with a field diaphragm for Koehler Illumination, use the following procedure to assure condenser centration and focus.
1. Close the Koehler field diaphragm on the base of the microscope so that its iris leaves are present within the field of view when looking into the eyepieces.
2. Use the condenser focusing knob, located on the left-hand side of the substage, to bring the leaves of the field diaphragm into sharp focus.
Condenser focusing knob Leaves are in sharp focus
Close the Koehler field diaphragm Close the Koehler field diaphragm
Leica DM 750 Manual 37
Koehler Setup (cont.)
3. Turn the condenser centering thumbscrews simultaneously to Center the image of the field diaphragm.
4. Open the field diaphragm until the iris leaves disappear just beyond the field of view.
Leica DM 750 Manual 38
Oil Immersion Technique
1. Find the field of view on the slide that you would like to inspect.
3. Place a drop of Leica Immersion Oil on the specimen slide on the area of the specimen that you were inspecting.
4. Swing the oil immersion objective in place
(the objective will be labeled "OIL")
2. Lower the stage to its lowest position using the coarse focus knob.
Leica DM 750 Manual 39
Oil Immersion Technique (cont.)
5. Raise the stage slowly using the coarse focus knob until the drop of oil on the specimen slide just comes in contact with the front of the oil immersion objective lens.
7. Look through the microscope and slowly rotate the fine focus knob to raise the stage until the specimen is in focus.
8. When finished using the oil immersion objective, be sure to clean the front of the objective, the specimen slide, and any other surfaces that the oil contacted using
the instructions in section "Care of the
6. Grip the knurled nosepiece ring and rock the objective back and forth to eliminate any air bubbles, then settle the oil objective into position with the oil between the front lens of the objective and the specimen slide.
Leica DM 750 Manual 40
Time Delay Shutoff
The Leica DM 750 is equipped with a time delay shutoff capability, which automatically turns the illumination off after 2 hours of no changes in the intensity control.
Ϙ
All four-position nosepiece stands have the time delay shutoff enabled as a default
(mostly educational applications).
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All five-position nosepiece stands have the time delay shutoff disabled as a default
(mostly clinical applications).
Changing the status of the time delay shutoff
1. Turn the power on.
2. Rotate the intensity control to full high intensity, then back to full low intensity all within 5 seconds.
Ϙ
The LED Illumination will flash to indicate the time delay shutoff status was changed.
Ϙ
The LED Illumination will flash two times slowly then stay on when you disable the
Time Delay Shutoff.
Ϙ
The LED Illumination will flash three times quickly then stay on when you enable the
Time Delay Shutoff.
When you turn the power off and then turn the power on again, the system will be in the last Time Delay Shutoff status (Enabled or Disabled) and you will not see any blinking.
Leica DM 750 Manual 41
Go!
Leica DM 750 Manual 42
Ready! Set! Go!
Now all you need to do is change objectives, set the condenser aperture (and field diaphragm if you have a DM 750 Koehler Stand) appropriately for the objective magnification you are using, and enjoy the view!
Leica DM 750 Manual 43
Care of the Microscope
Leica DM 750 Manual 44
General maintenance
General
•
Always carry the microscope using two hands. There is a handle on the back of the microscope and an undercut in the front for this purpose.
Ϙ
The cord wrap allows you to wrap the cord in such a way that only the length you need is extended.
Ϙ
Keep all optical components clean. Cleanliness is important for maintaining good optical performance.
Ϙ
The microscope should always be covered with the plastic dust cover (provided with the instrument) when it is not in use.
Ϙ
If any optical surface becomes coated with dust or dirt, clean the surface by blowing it off with a syringe or brushing it off with a camel hair brush before attempting to wipe the surface clean.
Ϙ
Optical surfaces should be cleaned with a lint-free cloth, lens tissue or a cotton swab moistened with methanol or a commercially available glass cleaner. Do not use alcohol.
Ϙ
It is very important to avoid the excessive use of solvents, so use them sparingly. The lint-free cloth, lens tissue or cotton swab should be moistened with solvent, but not be wet enough for the solvent to seep around the lens.
Leica DM 750 Manual 45
General maintenance (cont.)
Ϙ
No part of the microscope is quite so vulnerable to collecting dirt, dust, and oil as the front lens of the objective. Whenever you encounter lack of contrast, cloudiness or poor definition, carefully check the condition of the front lens with a magnifier.
Ϙ
The lower magnification objectives have fairly large front lenses and can be cleaned with a cloth or lens tissue wrapped around a finger and moistened with methanol.
Ϙ
Cleaning 40× and 100× objectives requires more care. Note: To achieve the high degree of flatness obtained with higher magnification objectives, the objective has a small concave front lens of fairly short radius or curvature. The surface of this front lens can be readily cleaned with a toothpick covered with a cotton tip, or with a small cotton swab. Moisten the cotton with methanol and squeeze it almost dry (do not use alcohol). Wipe the front lens lightly without applying undue force or scrubbing action.
Ϙ
Make sure that the cotton tip contacts the concave lens surface. Check the objective with a magnifier after cleaning.
If you need to remove the microscope’s viewing body, be careful not to accidentally touch the outer lens surface (located on the underside of the body). Fingerprints on this surface will reduce image clarity. This lens can be cleaned in the same manner as objectives and eyepieces.
Mechanical Maintenance
Ϙ
Use the dust cover. It is the most important way to keep the microscope in good mechanical and physical condition.
Ϙ
The Leica DM 750 has a durable finish. All non-optical parts of the instrument can be cleaned with methanol, naptha, or soap and water (do not use alcohol). Avoid the use of all other organic solvents when cleaning the instrument.
Illumination
Ϙ
The Leica DM 750 uses LED illumination.
Therefore, no lamp changing is required for the life of the microscope.
Leica DM 750 Manual 46
Dimensions
Leica DM 750 Manual 47
Leica DM 750 Manual 48
Leica DM 750 Manual 49
Leica DM 750 Manual 50
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