TurboVap® LV

Evaporation

© Biotage 2014 57

Biotage

®

SPE Dry 96 & 96 Dual

Sample Concentrator System

Biotage

®

SPE Dry 96

Sample Concentrator System

Part Number

SD-9600-DHS-NA

Description

SPE Dry 96 Sample Concentrator

System, 100/120V

SD-9600-DHS-EU SPE Dry 96 Sample Concentrator

System, 220/240V

SD-9600-DHS-T-NA SPE Dry 96 Sample Concentrator

System, with PTFE coated needles

(Top Head Only), 100/120V

SD-9600-DHS-T-EU SPE Dry 96 Sample Concentrator



Biotage

®

SPE Dry 96 Dry Dual

Sample Concentrator System

Part Number Description

SD2-9600-DHS-NA SPE Dry 96 Dual Sample Concentrator

System, 100/120V

SD2-9600-DHS-EU SPE Dry 96 Dual Sample Concentrator

System, 220/240V

SD2-9600-DHS-T-NA SPE Dry 96 Dual Sample Concentrator



SD2-9600-DHS-T-EU SPE Dry 96 Dual Sample Concentrator



Evaporation Brochure

For further information please visit w ww.biotage.com to request a copy of our Evaporation brochure.

58 © Biotage 2014

2014 Analytical Sample Preparation Catalog

TurboVap

®

Evaporators and Concentrators

TurboVap

®

Product Family

TurboVap 500 TurboVap 96 TurboVap II

C103199

C103200

C103201

C112175

C133718

C55941

C109301

Tube Racks

C48950

C44577

C45286

C44139

C45269

C44282

C44283

C44880

C47818

C47820

C48928

C48929

C59546

C61345

Part Number

TurboVap 500

C103202

C103203

TurboVap 96

C103263

C103264

TurboVap II

C103186

C103187

C103188

C103189

C103190

C103192

C103193

C103194

C103196

C103197

TurboVap LV

C103198

Description

TurboVap 500, 100/120V, 50/60Hz

TurboVap 500, 220/240V, 50/60Hz

TurboVap 96, 100/120V

TurboVap 96, 220/240V

TurboVap II, 200 mL with 0.5 mL Endpoint Stem, 100/120V








TurboVap II, 40 mL Dionex ASE Vial Compatible, 100/120V

TurboVap II, 40 mL Dionex ASE Vial Compatible, 220/240V

TurboVap LV without Racks, 100/120V

TurboVap LV without Racks, 220/240V

TurboVap LV, ASE, 40/60 mL vial, 100/120V

TurboVap LV, ASE, 40/60 mL vial, 220/240V

TurboVap LV, PTFE, 50 position, 100/120V

TurboVap LV, PTFE, 50 position, 220/240V

TurboVap LV, PTFE-Coated Upper Manifold Replacement Kit

TurboVap LV, Assy-Water Bath, PTFE Coated

Tube Rack (for 10 x 75 mm tubes P/N C48985) 250 tubes included






Tube Rack (16 x 100 mm-Prelude compatible)

Tube Rack (for 15 mL Conical Centrifuge Tubes P/N C44941) 125 tubes included

Tube Rack (15 mL Autotrace tubes with conical bottom, suitable for tubes C47816)

Tube Rack (10 mL conical tubes, suitable for tubes C47811)

Tube Rack (1.5-2.0 mL flip cap microcentrifuge tubes)

Tube Rack (1.5-2.0 mL screw cap microcentrifuge tubes)

Tube Rack (GC vials)

Tube Rack (ASE 40/60 mL IChem Vials)

For further information please visit w ww.biotage.com to request a copy of our Evaporation brochure.

TurboVap LV

© Biotage 2014 59

Solvent Evaporation

Fast, Reliable and Affordable

Fast, Automated and Safe Drying of Solvents

The Widest Range of Evaporation Solutions Available

CONTENTS

1

Biotage Evaporation System Selection Guide

2

TurboVap

®

Technology

3

TurboVap

®

Family

4

TurboVap

®

II

6

TurboVap

®

LV

8

TurboVap

®

500

10

TurboVap

®

96

12

Biotage

®

SPE Dry

14

Biotage

®

V-10 Evaporator

16

Accessories

17

Related Products

18

TurboVap

®

LV – Tubes and Racks Chart

20

Ordering Information

Biotage offers the widest range of solvent evaporation systems available. This guide presents the most suitable evaporation system for your research requirements, and answers common questions regarding service, maintenance and applications.

Evaporation systems can be found in virtually every type of laboratory, from drug discovery to analytical chemistry. Biotage evaporation instruments are commonly used in many applications and have been the market’s preferred choice for decades.

For synthesis reactions, the unique Biotage

®

V-10 Evaporator offers a speedy solution for both HPLC fractions and high boiling solvent removal. Its automatic capabilities makes the V-10 Evaporator a work horse for today’s drug discovery industries.

In clinical and hospital labs, general sample preparation is common practice and together with our ISOLUTE

®

and EVOLUTE

® products we offer a complete solution for everyday evaporation and sample preparation needs. TurboVap

®

LV can accommodate a variety of sample sizes and formats to adopt your most common work practices.

In the environmental field, larger samples and low concentrations are often big hurdles. TurboVap

®

II and TurboVap

®

500 offer a complete solution for both concentrations into smaller volumes or complete evaporation prior to analysis.

4 8

12 14

2

©2013 Biotage

Trademark Acknowledgement

The following trademarks are owned by Biotage AB: Advancer, Advancer 350, Advancer Kilobatch, AFFINILUTE, Biotage, Biotage ZIP, Endeavor, EVOLUTE, EVOLUTE EXPRESS, ExploraSep, Extrahera,

Firefly design , FLASH+, FlashMaster, FlashVac, Flash 75, Flash 150, Flash 400, Horizon, HPFC, HP-SIL, HP-Sphere, Initiator, Initiator Peptide Workstation, Initiator+, Initiator+ Alstra, Initiator+ Robot

60, Initiator+ Robot 8, Initiator+ SP Wave, Isolera, Isolera Dalton, Isolera Dalton Mass Detector, Isolera Dalton Nanolink, Isolera Dalton System, Isolera Four, Isolera LS, Isolera One, Isolera Prime,

Isolera Spektra, Isolera Spektra Four, Isolera Spektra LS, Isolera Spektra One, ISOLUTE, ISOLUTE Myco, ISOLUTE QuEChERS, IST, IST design , KILOPREP, KP-C18-HS, KP-C18-WP, KP-C4-WP, KP-

NH, KP-Sil, KP-Sphere, MIP Rule of 6, MIP[4]Process, MIP[4]Proteins, MIP4SPE, PathFinder, PRESSURE+, PRESSURE+ 48, PRESSURE+ 96, RapidTrace, RapidTrace+, RENSA, Resolux, Robot 60, Robot 8,

Samplet, SIM, SNAP, SNAP Ultra, SNAP XL, SP Wave, SP1, SP4, SPE Dry, SPE Dry 96, SPE Dry 96 Dual, SPx, Syro Wave, TurboVap, Universal Phase Separator, V-10, VacMaster, ZIF, ZIF-SIM, ZIP-Sphere,

1-Point Support.

Other product and company names mentioned herein may be trademarks or registered trademarks and/or service marks of their respective owners, and are used only for explanation and to the owners' benefit, without intent to infringe.

Solvent Evaporation – Fast, Reliable and Affordable | © Biotage 2013

System Overview

Biotage Evaporation System Selection Guide

Sample

Capacity

Sample

Volume

Key Applications Typical Solvents Heating Technology

TurboVap

®

II

6 50 mL or

200 mL

Food, water, soil and environmental solvent extraction.

Acetone

Acetonitrile

Methanol

Dichloromethane

Toluene

Water bath

(RT to 90 °C)

Gas vortex shearing technology sensor endpoint detection with either 0.5 mL or 1.0 mL endpoint stems.

TurboVap

®

II

ASE version

6 40 mL Food, water, soil and environmental solvent extraction.

Acetone

Acetonitrile

Methanol

Dichloromethane

Toluene

Water bath

(RT to 90 °C)


TurboVap

®

LV

50

TurboVap

®

LV

ASE version

24

From 1.5 mL to 30 mL

Forensic, clinical chemistry, food, and pharmaceutical laboratories for concentration of solvents following SPE cleanup of drug sampels or pesticide extracts.

40 mL or

60 mL ASE tubes

Food, water, soil and environmental solvent extraction.

Acetonitrile

Ethylacetate

Hexane

Methanol

Dichloromethane

Acetonitrile

Ethylacetate

Hexane

Methanol

Dichloromethane

Water bath

(RT to 90 °C)

Gas vortex shearing technology.

Water bath

(RT to 90 °C)


TurboVap

®

500

2 500 mL Larger samples, water testing in remote locations with simple lab equipment.

Comes with solvent recovery functionality as default.

Acetone

Hexane

Methanol

Dichloromethane

Water bath

(RT to 95 °C)


TurboVap

®

96

Biotage

®

Dry

SPE

96 x 2 Up to 2 mL Genomic and proteomic applications that require concentration of purified extracts. Widely used for drugs of abuse and other pharmaceutical SPE methods.

24, 48,

96, and

384 well microplates

Up to

10 mL

(depending on format)

Forensic, clinical chemistry, food, and pharmaceutical laboratories for concentration of solvents following SPE cleanup of drug samples or pesticide extracts.

Acetonitrile

DMSO (Dimethyl sulfoxide)

Ethanol

DMF (Dimethyl formamide)

THF (Tetrahydrofuran)

Acetonitrile

DMF

Methanol

Propan-2-ol

DMSO

Heat block

Gas (RT to

80 °C)


Heated gas delivery above and below wells speeds evaporation.

Biotage

®

V-10

1 Up to

12 mL

(depending on vial)

High BP solvents, reversed phase HPLC fractions.

Almost all solvents, especially good for:

DMF

DMSO

MeCN + water

MeOH + water

NMP

Heated air Patented 3-way evaporation including simultaneous heat, vacuum, and high speed vial rotation.

1

2

TurboVap

®

Technology

Highest Performance, Every Time

Starting with innovative engineering, TurboVap

®

systems from Biotage are designed with extensive customer input and testing. The result: industry-standard design and the highest performance in every way. TurboVap evaporators utilize a water bath system, providing higher efficiency and control and, unlike old heating blocks, consistently delivers fast and even results.

Highly Efficient, Patented Gas

Vortex Shearing Technology

The patented vortex evaporation design makes evaporation up to 10x faster compared to other techniques. The vortex created by the moving gas travels down the tube to the solvent surface, where it increases the gas/solvent interface—providing faster evaporation than conventional methods. The combination of gas flow and temperature control is used to optimize the sample drying. Nitrogen is recommended as the best choice of gas because it is inert and minimizes the risk of oxidation. A clean oil—free compressed air supply at 60 psi can be used if the sample is stable and/or nitrogen is not available.

Automated Evaporation Completion

The patented sensor technology alerts you with an audible alarm when evaporation is complete. This prevents the sample from going dry and volatiles from being lost. Sensor end-point detection is available with either 0.5 mL or 1.0 mL end-point stems.

Figure 1. Principle of the gas vortex shearing technology.

The position of the gas nozzle close to the tube wall is important for optimized evaporation.

Figure 2. The selection between

0.5 mL and 1 mL end-point detection is easily done with a quick modification of spacers.

0.5 mL mark and

0.5 mL end-point.

1 mL mark and 1 mL end-point.

Fast, Consistent and Even Results

TurboVap

®

500 does not require a fume hood or external gas supply for operation. It is designed as a closed system with up to 95% solvent recovery function for safe and economic waste handling.

Figure 3. The helical flow establishes a vortex action that promotes sample homogeneity and continuous rinsing of the tube wall.


TurboVap

®

Family

TurboVap

®

Family

Choose the Solution That’s Right for You

TurboVap

®

II

The TurboVap II Concentration Evaporator Workstation is a microprocessor-controlled concentrator for unattended, automated sample evaporation.

Unattended and automated

Optical sensor endpoint detection

6 individually controlled vial positions

No fume hood needed

TurboVap

®

LV

The TurboVap LV (Low Volume) Concentration Evaporator

Workstation offers many interchangeable tube racks giving you the flexibility for automated low volume sample preparation. The microprocessor controlled monitoring system regulates timed operation, water bath temperature, automatic gas shutoff and operational diagnostics.

50 positions with user selectable racks for greater flexibility

1.5–30 mL volume

TurboVap

®

500

The TurboVap 500 Concentration Evaporator Workstation is a closed-cell evaporation system that delivers automated sample concentration and solvent recovery using helical gas flow and sensor endpoint detection technology.

Solvent vapors are collected by the condensers on the side walls for waste or reclamation.

2 individually controlled positions

500 mL volume

0.5 or 1.0 mL endpoint stems

No fume hood needed

TurboVap

®

96

The TurboVap 96 Concentration Evaporator Workstation is a microprocessor-controlled evaporation system for simultaneous, automated and unattended concentration of multiple samples.

2 positions with individual gas regulation

Compatible with 96-well standard or deep well plates

3

Easy-to-use programming interface with individual tube control.

Glass window enables easy overview of the evaporation progress.

End-point detection terminates evaporation at

0.5 mL or 1.0 mL volumes.

Adjustable pressure regulator ensures optimal evaporation (not visible.)

4

TurboVap

®

II

Volume Range 50 mL or 200 mL

TurboVap

®

II increases the capacity and sample throughput in laboratories, eliminates concentration bottlenecks and maintains quality and lower costs.

This instrument has 6 sample positions and enables the user to evaporate to solid state or concentrate into a fixed end-point volume. The small footprint of TurboVap

®

II makes it the ideal choice in today's laboratories. It takes up only a fraction of the bench space used by conventional rotary evaporators and can even be used outside a fume cupboard by using the integral vent tube.

Applications

The TurboVap II Evaporation System efficiently concentrates samples prior to analysis by LC/MS or other analytical instruments. Optical sensors automatically stop gas flow when evaporation has reached its end-point, thereby eliminating the risk of drying and loss of volatile compounds. The TurboVap II is suitable for evaporation of large volumes after liquid-liquid extractions.

TurboVap II is available in a Dionex ASE* compatible setup which accommodate the use of six 40 mL stemmed and graduated Dionex ASE tubes. Upgrade kits are also available for converting your old TurboVap II.

*Dionex ASE is a registered trademark of Dionex/Thermo Fischer.


TurboVap

®

II

Evaporation Rates (180 mL solvent)

Solvent

Dichloromethane

Hexane

Acetone

Methanol

Acetonitrile

Toluene

Water

Bath temp.

(°C)

40

55

55

50

50

50

90

Gas pressure

(psi)

12

10

18

10

10

10

12

Approx. time (min.)

35

23

27

70

75

90

120

Specifications

Technology

Gas vortex shearing evaporation with sensor endpoint detection.

Available with either 0.5 mL or 1.0 mL endpoint stems

1–6

Number of samples

Timer range

Max. sample volume

Final endpoint volumes

Solvent reclamation

1 to 99 min. or 0.1 to 9.9 hrs.

200 mL

0.5 or 1 mL

–

Water bath capacity

6.4 L of distilled water

Water bath temperature

Ambient to 90 °C (upper temperature with sensors is 60 ºC)

Gas supply requirements Minimum inlet pressure

30 psi/2.1 bar. Maximum inlet pressure 80 psi/5.5 bar.

Gas consumption

Exhaust

At 11 psi: 0.08 cfm/nozzle or

2.3 L/min/nozzle

25 CFM blower with 5.1 cm/2" venting exhaust (tube supplied)

Electrical supply

220–240 V~, 50 Hz, 5 A (UK & EU)

100–120 V~, 50/60 Hz, 10 A

(USA & JP)

Max. power consumed

Dimensions (WxDxH)

Weight

Certifications

900 VA

53.8 cm x 30.5 cm x 30.5 cm

21.2" x 12" x 12"

18.4 kg/40.5 lbs

2006/95/EC Low Voltage Directive

2004/108/EC EMC Directive

93/68/EEC CE Marking Directive

Advantages

»

Proven equivalency with EPA methods

»

Patented vortex shearing technology

»

Patented sensor endpoint detection

– no monitoring required

»

Unattended operation for 1–6 samples

»

Controlled water bath is adjustable from ambient to 90 °C

»

Operators are free to perform other tasks while evaporation is running

»

User friendly displays and diagnostics

»

Convenient bench top size—no hood space required

Applications

»

Pharmaceutical biotech compounds

»

Clinical samples

»

Environmental samples

»

Forensic and crime samples

»

Drugs of abuse samples

»

Food and beverage analysis

»

Agrochemical samples

5

50 (standard) or 24

(ASE version) nozzles.

Each row of nozzles can be controlled individually, with indicator control for each line.

Adjustable pressure regulator ensures optimal evaporation (not visible.)

6

TurboVap

®

LV

Volume Range up to 60 mL

The TurboVap

®

LV evaporation system is an automated high speed, low volume sample concentrator. It is an efficient alternative to the inconvenient set-up, constant monitoring and long evaporation times that are characteristic of conventional techniques.

With the TurboVap

®

LV system, you simply “load and leave”. A water bath gives an even temperature and gas flow is delivered over a set time period. Turn on 1 to 5 manifolds depending on the number of samples and the timer will alert you when your samples are ready.

Applications

TurboVap LV is commonly used for evaporation of solvents following solid phase extraction clean-up of drug samples or pesticide extracts. It is ideal for sample volumes of 1 mL to

30 mL that need to be evaporated in GC vials, microcentrifuge tubes, conical bottom tubes or test tubes. It offers a high throughput solution with 50 sample positions using the standard range of Biotage racks.

TurboVap LV can be used in conjunction with the RapidTrace

®

+

Automated SPE workstation that collects extracts in 12 x 75 mm,

13 x 100 mm, or 16 x 100 mm test tubes. For acidic solutions,

Biotage offer PTFE coated nozzles to prevent corrosion. For volumes greater than 30 mL, an ASE compatible version of the

TurboVap LV is available for use in conjunction with Dionex ASE*

200 columns.

*Dionex ASE is a registered trademark of Dionex/Thermo Fischer.


TurboVap

®

LV


Solvent

Hexane

Acetonitrile

Dichloromethane

MeCN/water

Ethylacetate

Methanol/water

Methanol

Water

Bath temp.

(°C)

70

70

35

70

35

50

60

50

60

50

60

70

50

60

70

35

35

50

60

35

Gas pressure

(psi)

12

12

12

12

12

12

12

12

12

12

12

12

12

12

12

12

12

12

12

12

Approx. time (min.)

7

65

50

65

35

15

10

26

18

210

140

32

22

15

20

19

11

7

55

100

Specifications

Technology


Gas control

Timer range

Gas vortex technology

50

Gas can be turned on to each of five independent manifolds when working with fewer than fifty samples. Gas regulator and gas gauge range from

0 to 20 psi/0 to 2 bar.

1 to 99 minutes, 0.1 to 9.9 hours, or infinite

30 mL (60 mL with ASE version)

–





Gas supply requirements

Gas consumption

–

Ambient to 90 °C. Temperatures below ambient can be regulated using the cooling effect of the evaporation process.

Minimum inlet pressure 60 psi/4.1 bar

Maximum inlet pressure 100 psi/6.9 bar

At 10psi: 0.06 cfm/nozzle or 0.6 cfm/ row of 10 nozzles or 1.699 L/min/ nozzle.

At 15psi: 0.1 cfm/nozzle or 1 cfm/row of

10 nozzles or 2.83 L/min/nozzle

Exhaust


5.1 cm/2” venting exhaust (tube supplied)

220–240 V~, 50 Hz, 5 A (UK & EU)

100–120 V~, 50/60 Hz, 10 A (USA & JP)

Max. power consumed 900 VA


53.8 cm x 30.2 cm x 30.2 cm

21.2” x 11.9” x 11.9”

Weight

18.4 kg/40.5 lbs

Certifications




Advantages

»


»

Proven compliance with EPA methods

»

10x faster than centrifugal evaporators

»

3x faster than hot blocks

»

2x faster than conventional N

2

blow down

»

Unattended operation for up to 50 samples

»

Twelve racks available for tube sizes from 1.5 mL to 30 mL

»

Controlled water bath adjustable from ambient to 90 °C

»

Built-in nitrogen regulator

»

Unattended operation improves lab productivity

»

User-friendly displays and diagnostics

»

Convenient size – no hood space required

»

No tube balancing required

»

Easily swap racks to change between different tube sizes

»

Requires no additional equipment such as vacuum pumps and vacuum traps

Applications

»


»

Clinical samples

»


»


»


»


»


7

Vortex shearing motor requires no gas supply for operation.

Easy-to-use operation interface for time, temperature and endpoint detection settings.

Built-in chiller for solvent recovery.

Endpoint sensors for 0.5 or 1 mL evaporation.

8

TurboVap

®

500

Volume Range up to 500 mL

TurboVap

®

500 can evaporate volumes up to 500 mL down to complete dryness with flat bottomed vessels or to a selectable endpoint at 0.5 or 1 mL. A water bath is used to maintain stable temperature, while the cooled glassware condenser collects the solvent. The condenser technology allows for efficient and cost effective collection of hazardous waste. It also prevents losses of samples and allows for analysis to detect cross-contamination issues.

Applications

In environmental laboratories, concentration of large samples is often a time consuming process. TurboVap

®

500 efficiently concentrates organic solvent extracts to 0.5 or 1.0 mL and sounds an alarm when finished. This “load and leave” capability frees the analyst for more important work.

TurboVap 500 is also an excellent alternative to traditional rotary evaporators used in organic synthesis. With a 500 mL solvent capacity and water bath temperatures up to 95 °C, synthesis mixtures are processed quickly. The unique vortexing action maintains the components of interest in the solvent during evaporations and minimizes the “plating” of the compounds on the vessel walls. The open top cylindrical design of the sample tube, with a small stem at the bottom, provides an easy way to recover the products of interest when the evaporation is completed.


Advantages

»


»

Patented sensor endpoint detection

»

Operators are freed to perform other tasks, further improving lab productivity

»

No “bumping” means no re-working of samples, saving you money every week

»

Solvent vapor recovery system – laboratory emissions can be greatly reduced

»


»

Convenient bench top size – no hood space required

»

No need for fume hood

»

Portable – no gas required applications

»


»


»


»


»


»


»

Clinical samples

»

Remote locations

TurboVap

®

500

Specifications

Technology


Timer range


Evaporation rate



Gas vortex shearing technology

2

1 to 99 minutes

500 mL

Adjustable based on water bath temperature, fan speed and chiller or tap water temperature

Selectable. Automatic endpoints of 0.5 mL and 1.0 mL or dryness.

95% of solvent vapors under typical conditions



Exhaust

Ambient to 95 °C (upper temperature with sensors is 60 °C)

Internal fan, 4000–8000 rpm No external gas required

–

No gas required

Minimum operating pressure


Max. power consumed 900 VA


61 cm x 30.2 cm x 53.8 cm

24” x 11.9” x 21.2”

Weight

18.4 kg/40.5 lbs

Certifications

220–240 V~, 50 Hz, 5 A (UK & EU)

100–120 V~, 50/60 Hz, 10 A (USA & JP)




Evaporation Rates

Solvent

Dichloromethane

Hexane

Methanol

Acetone

Acetonitrile

Acetone/hexane (50:50)

Water

Methanol/water (50:50)

Solvent volume

(mL)

180

500

100

100

100

100

100

100

100

100

100

180

180

50

100

Bath temp. (°C)

58

58

75

58

58

58

58

56

38

38

38

38

38

58

58

Fan speed (rpm)

6000

6000

6000

6000

6000

6000

8000

8000

4000

6000

8000

4000

6000

6000

6000

Chiller temp.

(°C)

6

6

6

6

6

6

6

6

6

6

6

6

6

6

6

Approx. time

(min.)

23

9

100

43

13

33

25

12

25

4

7

15

10

7

37

9

10

Unique nozzle design ensures fast and efficient vortex shearing evaporation.

Easy-to-use interface for time and temperature operation.

Individual control for each compartment.

Adapters support different plates and depths.

Dual gas regulators, one for each plate, operated individually for greater flexibility.

TurboVap

®

96

Deep or Shallow 96-well Plates

The TurboVap

®

96 Concentration Workstation is a high speed concentrator designed to work with

96-well microplates and deep-well plates. It is an efficient alternative to the constant monitoring and long evaporation times that are characteristic of conventional techniques—with the added bonus of unattended operation.

The evaporation process is driven by Biotage’s patented gas-vortex action, further enhanced by a temperature controlled environment and adjustable gas flow rates. This combination saves time, bench space and operating costs while improving the evaporation speed and sample-tosample consistency.

Applications

TurboVap

®

96 has two independent compartments that accommodate 1 mL or 2 mL standard or deep well plates. Each compartment has independent control of gas flow and temperature. The flow rate of the gas is determined by the volume of solvent in the plate and the temperature values can be set with the help of an evaporation guide supplied. Nozzles are easy to clean and require minimal maintenance.

In genomic and proteomic applications, the evaporation of DMSO from plates is a common application. As is drying

HPLC aqueous solvent mixtures of methanol or acetonitrile.

TurboVap 96 complements automated 96-well SPE workstations that are widely used for drugs of abuse determination and other pharmaceutical SPE methods.


Advantages

»


»

Operators are free to perform more important tasks, further improving lab productivity

»

No “bumping” means no re-working of samples, saving you money every week

»


»

Convenient bench top size—no hood space required applications

»


»

Clinical samples

»


»


»


»


»


TurboVap

®

96

Specifications

Technology


Gas vortex shearing technology

2 x 96 well plates, standard or deep with adapters

Format

Timer range


8 x 12 well microplates and deep-well plates

1 to 99 minutes

<2 mL

Final endpoint volumes –

Capacity

2 standard 96 well microplates or deep-well plates

Heaters

Two internal individually controlled heaters. Each adjustable from ambient to 80±5 °C.


Range

Exhaust

Minimum operating pressure


Minimum inlet pressure

30 psi/2.1 bar, Maximum inlet pressure 80 psi/5.5 bar.

5–99 cfm

25 CFM blower with 5.1 cm (2”) venting exhaust (tube supplied)

70 psi

220–240 V~, 50 Hz, 3 A (UK & EU)

100–120 V~, 50/60 Hz, 6 A (USA & JP)


620 VA


30.5 cm x 30.5 cm x 61 cm

12” x 12” x 24”

Weight

Certifications

18.4 kg/40.5 lbs




Evaporation Rates

1

Solvent

Methanol

Water

Acetonitrile

Acetonitrile/water (50:50)

THF (Tetrahydrofuran)

DMF (Dimethylformamide)

DMSO (Dimethyl sulfoxide)

Solvent volume (uL)

500

500

500

1000

1000

1000

250

Heater temp. (°C)

70

80

70

60

50

80

70

2

Gas flow rate

(cubic ft/hr.)

60

60

60

70

50

70

60

1. Above studies were conducted with clean solvent in a standard 2 mL polypropylene 96 well, deep well microplate.

2. Not the temperature of the solvent in the well.

3. The conditions set on the TurboVap

®

(temperature and flow rate) may not be optimum settings for most efficient evaporation rates.

Time to evap. to dryness

3

12 min.

70 min.

25 min.

1.75 hrs.

10–15 min.

50 min.

3 hrs.

11

Individual control for upper and lower sections.

PTFE coated needles available as option when evaporating acids and harsh solvents.

Available with single or dual configurations.

Heated gas flow ensures faster evaporation, especially for high boiling point solvents.

Modular design for easy swap between different nozzle configurations.

Easy adjustment of nozzle heights for optimal evaporation conditions for all plates.

12

Biotage

®

SPE Dry

Deep or Shallow 24-, 48-, 96-, and 384-well Plates

Biotage

®

SPE Dry 96 and SPE Dry 96 Dual Sample

Concentrators are suitable for evaporation of microplate samples across a broad range of formats. By delivering heated gas above and below each well,

SPE Dry 96 systems dry samples quickly maintaining tight temperature control at user selected settings.

Designed for high throughput sample processing,

SPE Dry systems have simple front panel controls and use removable gas delivery assemblies for fast adjusting and cleaning.

Versatile Formats

SPE Dry is designed with novel technology and a simple interface to produce uniform and reproducable evaporation.

The design has a small footprint to fit easily in the fume hood.

The heating mechanism is designed to rapidly heat without overshooting the temperature, preventing degradation of temperature sensitive compounds.

Standard SPE Dry 96 and SPE Dry 96 Dual systems are supplied with stainless steel needles. For high purity work, or applications that require particularly aggressive solvents, PTFE coated needles are available. Both needle types can be individually replaced should they become damaged or corroded.

SPE Dry is available in single or dual format and adapts easily to

24-, 48-, 96-, and 384-well microplate formats.


Advantages

»

Efficient evaporation through heated gas flow above and below collection plate

»

Precise temperature control and gas flow with reproducible evaporation times

»

Compact design

»

Easy to use

»

Suitable for 24-,48-, 96- and 384-well microplates

Biotage

®

SPE Dry

Specifications

Technology


Format

Timer



Capacity

24, 48, 96 or 384 well plates


60 °C (upper head)

80 °C (lower head)

Gas supply requirements 1 bar to 4.1 bar (15 psi to 60 psi)

Gas flow

Operating flow rate 25 L to

100 L per minute (LPM)

Exhaust


Heated gas delivery above and below wells speeds evaporation

Models for one or two 96 well plates

8 x 12 well microplates and deepwell plates

Manual

10 mL collection plates

–



Weight

Certifications

–

220–240 V~, 50 Hz, 5 A (UK & EU)

100–120 V~, 50/60 Hz, 5 A

(USA & JP)

405 VA

25.4 cm x 38.1 cm x 40 cm (dual)

10” x 15” x 15.7”

24.2 cm x 30.6 cm x 40 cm (single)

9.5” x 12” x 15.7”

13.9 kg/31 lbs

EN61326 (1997 w/A1: 98 & A2: 01)

Class A

Evaporation Rates

1

(minutes)

40 °C

2

60 °C

2

Solvent

Methanol

Water

Methanol/water (50:50)

Propan-2-ol

Acetonitrile/water (50:50)

Methanol/acetonitrile (50:50)

Acetonitrile

Methanol/(v/v) 1% NH4OH

DMF

Methanol/dichloromethane (50:50)

Dichloromethane (v/v) 1% NH4OH

14

90

46

16.5

51

19

17

18

73

12

8.5

3

1 mL

4

60

24

20

28

28

165

95

26

105

15.5

11.5

11

46

28

11.5

31

12

12

13.5

33

7.5

6

3

1 mL

4

20

86

56

16.5

36

15

15.5

16

41

9.5

8

1. Experiments were conducted with flow rate at 50 liters/minute at the upper manifold and 30 liters/minute at the lower manifold. Drying gas used was compressed air. Only standard moisture trap was used within the system. Moisture content of ambient air will affect results.

2. Temperatures refer to the upper head. Bottom unit was 20 °C higher.

3.

4. 1 mL volumes were in a 2 mL collection plate.

13

14

Access to eight built-in methods.

-20 °C condenser captures solvent vapors.

Automatic condenser drain reduces maintenance.

Optional syringe pump used when re-dissolving.

Optional peristaltic pump for large sample volume transfer.

Biotage

®

V-10 Evaporator

Volume Range 1 mL to 12 mL

The Biotage

®

V-10 solvent evaporation system rapidly dries samples dissolved in both aqueous and organic solvents up to 20x faster than traditional rotary or centrifugal evaporators.

A patented combination of drying techniques, including highspeed vial rotation, uniform IR controlled heating, and vacuum drying, allows for both rapid evaporation and low temperature evaporation of high boiling solvents. Pre-defined evaporation methods have been optimized to protect the sample against overheating or bumping whilst still maintaining maximum evaporation speed.

Master of Versatility

The versatility of V-10 enables rapid evaporation of single samples up to 12 mL or larger pooled volumes by using the V-10 integrated pumps. The carousel accessory enables unattended evaporation of up to 16 samples.

V-10 is the ideal system for evaporation of solvents from prep

HPLC fractions. Speed up your analysis step with rapid evaporation directly into a suitable vial for down stream processing.

Fractionate directly into vials compatible with the V-10, or pool up the fraction racks and use our automation software Control

Centre to integrate the entire evaporation process.

With the use of a liquid handling robot, the instrument can combine multiple fractions/samples from different test tube racks and dry them into a single vial in one operation.


Biotage

®

V-10

With the patented technology in V-10, users can rapidly remove high-boiling solvents like water, DMSO or even NMP at mild temperatures. This ensures rapid evaporation without overheating delicate samples.

Since compounds are dried directly in a vial of choice, tedious tasks such as sample transfers, handling and reformatting are minimized, increasing productivity in the lab.

The Bump-free vortex evaporation prevents sample loss while the system’s precise temperature control eliminates sample overheating. All this to maximize sample recovery and fast turnarounds with the automatic end-of-run detection.

Advantages

»

Patented rapid three-way drying: heating, vacuum, rotation

»

Evaporation of high boiling solvents

»

Versatile and automated

»

Clean and safe operation

»

Eliminates reformatting

»

Enhanced green chemistry

»

Simple operation

»

Compact footprint

Applications

»

Pharmaceutical compound registration

»

Synthetic intermediate isolation

»

Synthetic intermediate re-dissolve

»

Evaporation of high boiling point solvents

Specifications

Technology


Sample delivery

Patented, 3-way evaporation including simultaneous heating, vacuum, and high speed vial rotation

1–16 (with carousel)

5 mL syringe pump and/or peristaltic pump

30 mL (single vial)


Vial size compatibility

4 mL (3 variants)

8 mL HPLC vial

16 ml vial

20 mL scintillation vial

30 mL scintillation vial


User selectable volumes


Up to 98% of solvent vapors under typical conditions

Nitrogen (when using pump for solvent delivery)


Exhaust

Vacuum operating range


Double trapping

Ambient to 2 mbar



Weight

Certifications

220–240 V~, 50 Hz, 8 A (UK & EU)

100–120 V~, 50/60 Hz, 15 A

(USA & JP)

1700 VA

49 cm x 54 cm x 53 cm

19.3” x 21.3” x 20.9”

50 kg/110 lbs

EN 61010-1:2001 Low Voltage

Directive;

EN 61326 -1 EMC Directive;

UL 61010-1:2004;

CAN/CSA C22.2 No 61010-1-04 FCC part 15


Solvent

NMP

DMSO

DMF

Pyridine

Water

Methanol

Cydohexane

Boiling Point (°C)

202

180

150

115

100

65

81

V-10 Evaporation (min)

18

15

4

5.5

9

3

2.5

Centrifugal Evaporation

(min)

N/A

180

90

70

140

70

40

Blowdown Evaporation

(min)

N/A

N/A

N/A

N/A

240

40

20

15

16

Accessories

Vial Adapters for Biotage

®

V-10

Several vial adapters are available for 4 mL, 8 mL, and other vial types including 4 mL straight side vials.

Vial Carousels for Biotage

®

V-10

For automated drying of several solutions, vial carousels are available for 4 mL vials and 20 mL scintillation vials.

Gilson

®

Liquid Handler for Biotage

®

V-10

The Gilson GX-271 liquid handler enables fully automated evaporation together with V-10. Evaporate chromatography fractions directly from the chromatography system rack without manual intervention.

High-vacuum Pumps for Biotage

®

V-10

For the enhanced evaporation of high boiling solvents (DMSO,

NMP), an external high-vacuum pump is necessary. Biotage offers two models, the oil-based Vacuubrand RZ 2.5 and the oil-free Edwards XDS5.

TurboVap

®

II ASE Conversion Kit

With the Dionex ASE and the ASE Compatible TurboVap II, a six position rack will accommodate the use of 40 mL stemmed tube with 1 mL endpoint. This tube is available through Dionex

Corporation. For further information please contact Dionex at

1-800-523-4571.

TurboVap

®

LV ASE Conversion Kit

Convert TurboVap LV to 24 sample positions suitable for ASE

I-Chem vials. The kit comes with a rack with a removable shelf that allows the user to convert the rack from 40 mL to 60 mL vials and vice versa. This rack can also be used for the graduated, stemmed 40 mL Dionex vials.


Related Products

RapidTrace

®

+

RapidTrace

®

+ is a robust automated platform for sample preparation designated for solid phase extraction, SPE. It is used in pharmaceutical, clinical and forensic laboratories to develop and process rugged and reliable methods. RapidTrace+ eliminates SPE bottlenecks so you can realize the full benefits of today’s powerful analytical instruments.

Biotage

®

PRESSURE+ 48 & 96

PRESSURE+ manifolds deliver parallel positive pressure processing for 96 well plates in 1 mL 3 mL and 6 mL column formats. The systems utilize a consistent, uniform flow of positive pressure to move both low and high viscosity liquids through SPE plates and columns. Each port of the PRESSURE+ manifold independently maintains constant pressure, increasing the overall reproducibility of analyte recoveries. This unique design allows for partially populated racks to be used without sacrificing extraction efficiency.

Isolera

™

Spektra One/Four/LS

Isolera

™

is a family of flash purification systems with intelligent features that enable chemists to easily achieve better separations at scales from milligrams to over 150 g. The new Isolera Spektra enables chemists to purify more compounds in less time and reduce post-process purity analysis. Isolera Spektra doubles chemists’ productivity while slashing purification costs with 20% or more.

Accessories

17

TurboVap

®

LV – Tubes and Racks Chart

Match Your Tube to the Outline and Order the Corresponding Rack or Tubes

18

(Scale 1:1)

Dimensions (mm)

Maximum recommended volume

LV rack part number

Test tube part number

Packsize

20 x 150

30 mL

C44282

C40709

250

16 x 125

16 mL

C45269

C45273

250

16 x 100

12 mL

C44139

C40708

250

13 x 100

7 mL

C45286

C40707

250

12 x 75

4 mL

C44577

C44651

250

10 x 75

4 mL

C48950

C48985

250


Tube Matching Guide

Snap cap reference nr

Biotage part number

Kimble #73837

C44942 (case of 500)

Tube reference number

Max recommended volume



Kimble #45165

15 mL

C44880*

N/A

Kimble #73790

15 mL

C44880*

C44941 (vials only)

*Tubes (#C44941, 125 pieces) and 125 caps are included with purchase of this rack.

**Tubes not included with purchase of this rack.

Corning #8080

15 mL

C44880*

N/A

Kimble #73785

10 mL

C47820

C47811

Biotage #47816

15 mL

C47818**

C47816

Cap type

Max recommended Vol.



Screw Cap

1.5 mL

C48929

N/A

Flip Cap

1.5 mL

C48928

N/A

Screw Cap

2 mL

C48929

N/A

Flip Cap

2 mL

C48928

N/A

19

20

Ordering Information

TurboVap

®

II

Product Part Number

110V AC

TurboVap II Concentration Workstation with 200 mL tubes and 1.0 mL stems (110/120V) Includes: set of glassware (6 ea.), auxiliary rack for holding tubes,

12.5’ of 2” diameter duct hose, clear bath and operator’s manual







C103187

C103186

C103188

C103189

220V AC

TurboVap II Concentration Workstation with 200 mL tubes and 0.5 mL stems (220V) Includes: set of glassware (6 ea.), auxiliary rack for holding tubes,








C103190

C103192

C103193

C103194

ASE Versions

ASE compatible TurboVap II Concentration

Workstation to accommodate 40 mL/1.0 mL Dionex tubes (110/120V) Includes: auxiliary rack for holding tubes, 12.5’ of 2” diameter duct hose, clear bath and operator’s manual

ASE compatible TurboVap II Concentration

Workstation to accommodate 40 mL/1.0 mL Dionex tubes (220V) Includes: auxiliary rack for holding tubes, 12.5’ of 2” diameter duct hose, clear bath and operator’s manual

TurboVap II ASE Upgrade Kit

C103196

C103197

C64676


Vials

Evaporation tube (200 mL, 1 mL endpoint, 12/case) C128506

Evaporation tube (200 mL, 0.5 mL endpoint,

12/case)

C128507

Evaporation tube (50 mL, 1 mL endpoint, 12/case) C128511

Evaporation tube (50 mL, 0.5 mL endpoint, 12/case) C128508

Evaporation tube (50 mL, centrifuge, 12/case) C128512

TurboVap

®

500

Product

TurboVap 500, 100/120V, 50/60Hz

TurboVap 500, 200/220V, 50/60Hz

Vials

Evaporation tube (500 mL, 1 mL endpoint, 2/pk)

Evaporation tube (500 mL, 0.5 mL endpoint, 2/pk)

Evaporation tube (500 mL, flat bottom, 2/pk)

Spare Parts

TurboVap 500 condenser (1/pk)

Part Number

C103202

C103203

C128515

C128510

C128514

C128509

TurboVap

®

LV

Product

TurboVap LV, 110V (rack not included)

TurboVap LV, 220V (rack not included)

ASE compatible TurboVap LV, 40/60 mL vial, 110V

ASE compatible TurboVap LV, 40/60 mL vial, 220V

TurboVap LV ASE Upgrade Kit

TurboVap LV-PTFE, bath, manifold, 50 pos, vial

120V

TurboVap LV-PTFE, bath, manifold, 50 pos, vial

220V

Vials

Test Tubes (10 x 75 mm, uncapped, 1000/case)






Test Tubes (10 mL conical, capped, 125/case)

Test Tubes (15 mL conical, capped, 125/case)

Test Tubes (polypropylene snap caps for 15 mL conical bottom tubes, 500/case)

C48985

C44651

C40707

C40708

C45273

C40709

C47811

C44941

C44942

Part Number

C103198

C103199

C103200

C103201

C60911

C112175

C133718




Racks

Tube Rack (1.5–2.0 mL flip cap microcentrifuge) C48928

Tube Rack (1.5–2.0 mL screw cap microcentrifuge) C48929

Tube Rack (10 x 75 mm)




Tube Rack (16 x 100 mm, prelude compatible)

C48950

C44577

C45286

C44139

C44283



Tube Rack (10 mL conical)

Tube Rack (15 mL conical)

Tube Rack (15 mL volumetric autotrace)

Tube Rack (ASE i-Chem Vials), TurboVap LV

C45269

C44282

C47820

C44880

C47818

C61345

TurboVap

®

Product

96

Product

TurboVap 96, 100/120V, 50/60 Hz

TurboVap 96, 220/240V, 60/60 Hz

Biotage

®

V-10 Evaporator

110V AC (USA)

with syringe pump with peristaltic pump with dual pumps with syringe pump and carousel drive unit with peristaltic pump and carousel drive unit with dual pumps and carousel drive unit

230V AC (EU)


100V AC (JPN)


Part Number

C103263

C103264

Part Number

EV10-1SX

EV10-1PX

EV10-1DX

EV10-1SC

EV10-1PC

EV10-1DC

EV10-2SX

EV10-2PX

EV10-2DX

EV10-2SC

EV10-2PC

EV10-2DC

EV10-3SX

EV10-3PX

EV10-3DX

EV10-3SC

EV10-3PC

EV10-3DC


Accessories

4 mL vial upper adapter kit 5 pcs (type A), 8.5 mm neck inner diameter

4 mL vial upper adapter kit 5 pcs (type B),

11.1 mm neck inner diameter

4 mL vial upper adapter kit 5 pcs (type C),

12.0 mm neck inner diameter

4 mL vial lower adapter kit 2 pcs (type A),

14.8 mm vial diameter

4 mL vial lower adapter kit 2 pcs (type B),


4 mL vial lower adapter kit 2 pcs (type C),


Start up kit – Gilson

®

GX 271 (also 215,222)

V-10 carousel vial holder, 20 mL

V-10 carousel vial holder, 4 mL 14.5 mm

(type A/B)

V-10 carousel vial holder, 4 mL 16.5 mm (type C)

Gilson

®

GX 271 liquid handler for V-10

410647

410648

410649

411031

411032

411033

411037

411181

411182

411183

411816

Biotage

®

SPE Dry 96


Single Plate

SPE Dry-96 Dual Heat Source (DHS), NA 110V

SPE Dry-96 Dual Heat Source (DHS), EU 220V

SD-9600-DHS-NA

SD-9600-DHS-EU

Dual Plates

SPE Dry-2x96 Dual Heat Source (DHS), NA

110V

SD2-9600-DHS-NA

SPE Dry-2x96 Dual Heat Source (DHS), EU

220V

SD2-9600-DHS-EU

Additional configurations are available. Please contact a Biotage representative for more information.

Accessories Single Plate

Spare upper needle head assembly with 96 straight needles, PTFE coated

Spare upper needle head assembly with 384 straight needles, PTFE coated

Top needle assembly (PTFE coated) - 24 channel

Accessories Dual Plate

Spare upper needle head assembly, 2 x 96 straight needles, coated

Spare upper needle head assembly, 2 x 384 straight needles, coated

SD-9601-T

SD-9601-384-T

SD-9601-T-24

SD2-9601-T

SD2-9601-384-T

21

Manual Sample Processing

Products and Accessories

© Biotage 2014 49

Biotage

®

PRESSURE+ 48 and PRESSURE+ 96

Positive Pressure Manifolds

Biotage

®

PRESSURE+ 96


PPM-96 PRESSURE+ 96 Positive Pressure Manifold 96 position. Includes 1 x 1 mL 96 well collection plate, 1 x 2 mL 96 well collection plate,

1 x 10 mL 24 well collection plate (waste plate),

96-column sealing gasket, gas supply adaptor kit (6' of 1/8" i.d. polyethylene tubing and 1/8" and 1/4" NPT connectors),

User Manual CD-ROM.

Accessories

PPM-A96-GSKT PRESSURE+ 96 Sealing Gasket 96 position

PPM-A96-1024 PRESSURE+ 96 Collection Tray 10 mL 24 well

PPM-GA

121-5202

121-5203

PRESSURE+ Gas Supply Adaptor for all models

96-well collection plate, 1 mL, pack of 50

96-well collection plate, 2 mL, pack of 50

Biotage

®

PRESSURE+ 48


PPM-48 PRESSURE+ 48 Positive Pressure Manifold 48 position. Includes Sealing gasket 48 position, gas supply adaptor kit (6' of 1/8" i.d.

polyethylene tubing and 1/8" and 1/4" NPT connectors), waste bin rack, waste bin inserts,

User Manual CD-ROM. Racks must be ordered separately.

Racks

PPM-A48-1RCK PRESSURE+ 48 SPE Column Rack 1 mL



PPM-A48-1275 PRESSURE+ 48 Collection Rack 12 X 75 mm

PPM-A48-13100 PRESSURE+ 48 Collection Rack 13 x 100 mm

PPM-A48-1232 PRESSURE+ 48 Sample Vial Rack 12 x 32 mm

PPM-A48-16100 PRESSURE+ 48 Collection Rack 16 x 100 mm

Accessories

PPM-A48-GSKT PRESSURE+ 48 Sealing Gasket 48 position

PPM-A48-WST PRESSURE+ 48 Waste Bin Inserts

PPM-GA PRESSURE+ Gas Supply Adaptor for all models

Service Options


SER-PPM-IN PRESSURE+ Manifold Service Installation

SER-PPM-PL PRESSURE+ Manifold Platinum

Service Contract - 1 year

SER-PPM-SLVR PRESSURE+ Manifold Silver Repair Depot Service

Contract - 1 year

SER-PPM-FYMP PRESSURE+ Manifold 9 month extended warranty (1 year total)

50 © Biotage 2014


Biotage

®

VacMaster

TM

-10 and -20

10 or 20 position vacuum manifolds for SPE, SLE and filtration applications.

Biotage

®

VacMaster

TM

-10 & -20

Sample Processing Manifolds

PTFE Stopcock Option and Spare Parts


121-0009

121-0001

121-0002

121-0003

121-0004

Universal PTFE stopcock

PTFE stopcock/needle unit

PTFE needle

3

Stainless steel needle

4

1

2

Stainless steel needle retainer

121-0005

121-0009-S

Port sealing plugs

PTFE stopcock - pressure positive

20

10

30

10

Qty.

10

10

10

VacMaster-10 Sample Processing Manifold


121-1010 VacMaster-10 Sample Processing Manifold

(with 10 mm rack)


(with 12 mm rack)


(with 16 mm rack)

VacMaster-10 Replacement Parts


121-1039 Replacement tank with fittings

121-1045 Replacement lid with fittings

121-1030

121-1132

121-1133

121-1134

Silicone Lid Gasket

Replacement rack, 10 mm



Qty.

1

1

1

1

1

1

VacMaster-20 Sample Processing Manifold


121-2010 Sample Processing Manifold

(with 10 mm rack)


(with 12 mm rack)


(with 16 mm rack)

VacMaster-20 Replacement Parts


121-2068 Replacement tank with fittings

121-2075 Replacement lid with fittings

121-2059

121-2161

121-2162

121-2163

Silicone Lid Gasket




Qty.

1

1

1

1

1

1

VacMaster Accessories


121-0010 PTFE 'T' valve (for connecting 2 tanks)

Qty.

1

1 2 3 4

© Biotage 2014 51

Biotage

®

VacMaster

TM

-96

Sample Processing Manifold

Biotage

®

VacMaster

TM

-96

Vacuum manifold for processing 96-well SPE, SLE or filtration plates.



(without vacuum control)

Vacuum Control Options

121-9601

121-9602

VacMaster VCU-1 Vacuum Control Unit

VacMaster VCU-2 Vacuum Control and

Generation Unit

96 Well Collection Plates - Deep Well

121-5202

121-5203

Collection plate, 1 mL

Polypropylene, Square well - 2 mL

Qty.

1

50

50

1

1

Replacement Parts


121-9612 VacMaster-96 replacement gasket

121-9613

121-9614

121-9610

121-9615

121-9611

VacMaster-96 replacement o-ring

Collection plate spacer (2 mm) for deep well collection plates

Array insert (6 mm), acetal

Collection plate spacer (29 mm) for shallow well collection plates

VacMaster-96 insert (12 mm) for

“shallow skirt” plates

5

6

1

2

3

4

Qty.

1

1

1

1

1

1

5

6

4

1

2

3

52 © Biotage 2014


Sample Processing Accessories

Biotage

®

VacMaster

TM

Drying Adaptor

Connect to laboratory air or nitrogen supply to dry 10 or 20

SPE columns simultaneously.


124-1001 VacMaster-10 Drying Adaptor for 1,3 & 6 mL columns

124-2001 VacMaster-20 Drying Adaptor for 1,3 & 6 mL columns

Qty.

1

1

Biotage

®

VacMaster

TM

Trap Kit

Connect between VacMaster (-10, -20, or -96) and vacuum source to collect waste. 1 L volume.


121-2095 VacMaster trap kit (complete)

Qty.

1

Biotage

®

VacMaster

TM

Large Volume Extraction (LVE) Kit

For unattended loading of large volume samples. Inert PTFE tubing prevents sample contamination.


121-2090 VacMaster LVE kit (complete) for 1, 3 and 6 mL columns

Qty.

1

© Biotage 2014 53

Gravity Rack

Gravity Rack

Process up to 20 samples simultaneously under gravity.


123-2016 Gravity Rack with 16 mm collection tube rack

123-2019 Gravity Rack with 19 mm collection tube rack

Accessories

121-0009

121-0001

121-0002

121-0003

121-0004

Universal PTFE stopcock

PTFE stopcock/needle unit

PTFE needle unit

Stainless steel needle

Stainless steel needle retainer

Qty.

1

1

10

10

10

20

10

54 © Biotage 2014


Sample Preparation

Accessories

© Biotage 2014 45

Accessories

For SPE and Filtration

ISOLUTE

®

Column Adaptors

Column adaptors attach empty reservoirs, filtration columns and depth filters to SPE columns.


120-1100 PTFE Adaptor - 1, 3 & 6 mL and XL

(columns A, B, C, G & H)

120-1101

120-1102

PE Adaptor - 1, 3 & 6 mL

(columns A, B & C)

PE Adaptor - 15 & 25 mL

(columns D & E)

120-1103

120-1104

PE Adaptor - 70 mL

(column F)

PTFE Adaptor - 6 mL

Glass columns

1

2

3

4

5

Qty.

10

10

10

10

10

ISOLUTE

®

Depth Filter Reservoirs

Reservoirs with pre-fitted depth filters remove particulate matter from aqueous samples, and prevent column blockage. Also allow for separate analysis of particulate compounds.

1

2


120-1003-CD ISOLUTE depth filter, 6 mL

1

120-1009-FD ISOLUTE depth filter, 70 mL

2

Qty.

100

50

ISOLUTE

®

Column Caps

Seal prepared columns, samples loaded columns or immunoaffinity columns for transportation or storage.


1201-0120 Bottom Luer cap, fits all columns

1201-0121-A Top cap, 1 mL column

1201-0122-B Top cap, 3 mL column

1201-0123-C Top cap, 6 mL column

1201-0125-H Top cap, 10 mL –

XL column

1201-0126-D Top cap, 15 mL column

1201-0127-E Top cap, 25 mL column

1201-0128-F Top cap, 70 mL column

D

E

F

Column

Type

All

Qty.

100

A

B

C

G & H

100

100

100

100

100

100

100

3

5

4

1

2

46 © Biotage 2014


Accessories


ISOLUTE

®

Empty Reservoirs

Stack above SPE columns to increase the reservoir volume.

Part Number

120-1001-A

120-1002-B

120-1003-C

120-1006-D

120-1007-E

120-1009-F

Description

ISOLUTE Reservoir 1 mL






Qty.

100

100

100

100

100

50

ISOLUTE

®

Frits

20 µm porosity sintered polyethylene frits to fit all SPE column sizes.


120-1031-A ISOLUTE Frit, 6 mm

(

1

/

4

"

) diameter – 20 µm PE

120-1032-G ISOLUTE Frit, 6 mm

(

1

/

4

"


120-1033-B ISOLUTE Frit, 9 mm

(

3

/

8

"


120-1034-H ISOLUTE Frit, 9 mm

(

3

/

8

"


120-1035-C ISOLUTE Frit, 13 mm (

1 diameter – 20 µm PE

/

2

"

)

120-1036-D ISOLUTE Frit, 16 mm (

5 diameter – 20 µm PE

/

8

"

)

120-1037-E ISOLUTE Frit, 20 mm

(

13

/

16

"


120-1038-F ISOLUTE Frit, 27 mm

(

11

/

16

"


Column

Type

A (1 mL)

G (XL)

B (3 mL)

H (XL)

C (6 mL)

D (15 mL)

E (25 mL)

F (70 mL)

Qty.

100

100

100

100

100

100

100

100

ISOLUTE

®

Filtration Columns

Pre-fitted with two 20 µm polyethylene frits. Use as standalone filters or stacked above an SPE column.


120-1021-A ISOLUTE double fritted filtration column,

1 mL–20 µm PE

120-1022-B ISOLUTE double fritted filtration column,

3 mL–20 µm PE

120-1023-C ISOLUTE double fritted filtration column,

6 mL–20 µm PE

120-1024-H ISOLUTE double fritted filtration column,

10 mL–20 µm PE

120-1025-D ISOLUTE double fritted filtration column,

15 mL–20 µm PE

120-1026-E ISOLUTE double fritted filtration column,

25 mL–20 µm PE

120-1028-F ISOLUTE double fritted filtration column,

70 mL–20 µm PE

Qty.

100

100

100

50

100

100

50

ISOLUTE

®

Sodium Sulfate

Drying Cartridges

Dry organic solvents with this easy to use format (contains 2.5 g of high purity sodium sulfate per cartridge). Stack beneath SPE columns during elution for efficient in-line solvent drying.


802-0250-M ISOLUTE Sodium

Sulfate Drying Cartridge

Qty.

50

© Biotage 2014 47

Accessories


ISOLUTE

®

Filtration Plates

Each well contains a 20 µm polyethylene frit for high throughput filtration applications.

Part Number

120-1022-P01

Description

Filtration plate, fixed well,

2 mL–20 µm

Qty.

1

Plate Sealing Accessories

Seal wells and Luer outlets of ISOLUTE-96 fixed well plates.

Piercable sealing cap can also be used to seal collection plates.


121-5204 Piercable Sealing Cap

121-5205 Luer Cap Mat

Qty.

50

25

Deep Well Collection Plates

1 or 2 mL volume per well, constructed from high purity, solvent resistant polypropylene. Seal with piercable sealing cap.


121-5202 Collection plate, 1 mL

121-5203 Collection plate, 2 mL

Qty.

50

50

Array Accessories

Populate the reusable base plate to transfer individual Array wells into microplate format. Seal any unused positions with base plate plugs. Align wells and depopulate base plate after use with the well removing tool. Use Luer adaptors to process individual Array wells using a VacMaster-10 or -20 vacuum manifold.


120-1000-P01 ISOLUTE Array base plate

120-1200

120-1201

120-1202

ISOLUTE base plate sealing strips

(strips of 8)

Luer Adaptors

(to fit any vacuum manifold)

Well removing tool

Qty.

1

50

25

1

48 © Biotage 2014


Automated Sample

Processing Products

© Biotage 2014 55

RapidTrace

®

+

Automated High Throughput Solid Phase Extraction

Closed fluid path system with segregated waste lines maintain integrity of sample.

Reagent mixing chamber allows automated online reagent blending in the method.

Eight solvent/reagent lines for maximum methods flexibility.

Positive pressure, stepper motor syringe pump delivers precise flow rates.

Magnetically encoded racks of routine samples.

Compatible 1, 3 and 6 mL version SPE columns.


C50000 RapidTrace+ Workstation,

1 mL and 3 mL

(10 columns)

C125713 RapidTrace+ Workstation,

6 mL (5 columns). 3 mL and 1 mL operation possible by purchasing appropriate plunger (see accessories brochure)

C52006

C52689

RapidTrace Start-Up Kit with Software

RapidTrace Notebook Controller

RapidTrace

®

+ Brochure

For further information please visit w ww.biotage.com to request a copy of our RapidTrace brochure.


Racks (Each RapidTrace+ requires one rack)

C50974 13 x 100 mm Sample Tube

12 x 75 Fraction Tube

C50976 13 x 100 mm Sample Tube

12 x 75 Fraction Tube

C58309

C56786

C56536

C133968

13 x 100 mm Sample Tube

13 x 100 mm Fraction Tube





(9 Position Chilled Rack)

40 mL Sample Flask (5 position)

16 x 100 Fraction Tube (10 position)

1

1

1

Qty.

1

Pk/5

1

56 © Biotage 2014


RevisedPages.qxp:Brochure 19/6/13 16:45 Page 2

RapidTrace

®

+

Automated SPE Workstation

Instruments

RapidTrace+

Accessories

Racks

Vial Adapters

Test Tubes

O-Rings

Miscellaneous

RT+_10.2011.qxp:Brochure 9/29/11 11:16 AM Page 2

Automate

Your Extractions

Increase your throughput and productivity

The RapidTrace

+

is a robust automated platform for quickly developing rugged, reliable SPE methods in regulated pharmaceutical, clinical and forensic laboratories. The RapidTrace

+

eliminates

SPE bottlenecks so you can realize the full benefits of today’s powerful analytical instruments.

The SPE Landscape

Advanced bioanalytical techniques like LC/MS/MS and GC/MS are meeting the increasing demand for greater speed and specificity in toxicological screening.

Conventional sample preparation methods have not kept pace. Solid phase extraction (SPE) in particular, an essential step in the analysis of many biomolecules, has become a throughput limiting factor in many laboratories.

The New Solution

The RapidTrace+ is the perfect complement to high speed analytical techniques, a powerful high-throughput workstation dedicated specifically to SPE. In its fully modular configuration, a ten module workstation can process up to 100 samples per hour.

The RapidTrace+ delivers:

• More samples per headcount

• Faster turnaround of results

• Improved quality, precision, accuracy and sensitivity

• Consistency of preparation

• Compliance with regulatory requirements

• Improved safety

• Quick response to peak or unexpected work loads

contents

RapidTrace+

Advantages

Specifications

Accessories & Related Products

Upgrades


page

5

6

7

3

4

7

2


Closed fluid path system with segregated waste lines maintain integrity of sample

Eight solvent/reagent lines for maximum methods flexibility

Reagent mixing chamber allows automated online reagent blending in the method

Positive pressure, stepper motor syringe pump delivers precise flow rates

Magnetically encoded racks of routine samples

RapidTrace

®

+

Intuitive SPE Automation

Compatible with 1, 3 and

6 mL version SPE columns

Rapid, Automated Sample Preparation for Routine

Processing and Method Development

The RapidTrace+ is designed for advanced processing and the rapid development of rugged sample preparation methods. It is extremely reliable and precise, incorporating easy-to-use features for quick method optimization. RapidTrace+ has been successfully implemented in a variety of laboratories all over the world.

The Biotage RapidTrace+ eliminates manual sample processing bottlenecks so that analysts can realize the full benefits of today’s powerful analytical instruments. RapidTrace+ is the perfect compliment to highspeed analytical techniques, a powerful high-throughput workstation dedicated to unattended automated sample preparation.

The enhanced RapidTrace+ broadens usability and increases instrument performance.

Fast and Easy Method Development with Easy-to-Use Software

Utilizing the familiar Microsoft

®

Windows

® interface, operators can easily implement a structured, rational strategy for method-development to determine optimum processing conditions for each sample. The methods can be written in minutes and recalled in seconds. Optimization of processing conditions is possible in a fraction of the time compared with conventional manual techniques. Column formats, reagents, concentrations and flow rates can be thoroughly explored as the system allows for incremental manipulation of any parameter (eg. pH, solvent concentration, cartridge type, flow rate etc) in order to determine effects on recovery and precision. Operators can insert, delete or manipulate steps and easily adjust parameters.

Methods can be assigned to an individual sample or by module. The methods stored in the RapidTrace+ controller can be easily transferred to other RapidTrace+ workstations.

Additionally, methods can be saved and run concurrently or true, unattended, “multi-method” sample processing.

This unique and rational method design capability dramatically speeds the development of rugged and reliable methods.

Instruments

3


Technological Advantages for High Performance

Unmatched Performance and Throughput

The RapidTrace+ reduces timeconsuming manual sample preparation methods and ensures that processing times match analytical capabilities.

Every step of the sample preparation process is automated and rigorously controlled to give you a level of reproducibility unmatched by manual techniques.

All sample and solvent flow rates are individually controlled under positive pressure. Each step in the processing can be optimized for maximum accuracy and precision.

The improved throughput and workflow has been noted by customers reporting a 92% reduction in analyst time and a 72% reduction in extraction time for a 75 sample extraction, comparing the RapidTrace+ procedure against a manual vacuum manifold.

•

NEW!

Compatible with 1, 3, and 6 mL SPE Columns

Increased bed height enables the use of a wider range of

SPE cartridges including all ISOLUTE

® and EVOLUTE

® bed masses allowing for flexibility in methodology.

•

NEW!

Larger sample volumes

A new 5 position rack for 40 mL scintillation vials allows for larger sample volumes to be processed, often desired for food and environmental applications.

•

NEW!

Delay Start feature

Easily leave extracted samples unattended. Extracts are freshly prepared when you return.

• Modular design allows for up to 10 linked units

The Modular design allows users to start with one module and expand up to 10 linked units. Modules within a group can run different methods independently and if one module is shut down for operator attention, other units remain operational so that sample throughput is not halted.

• Accelerated methods development and optimization

Utilizing the easy-to-use interface, it is simple to implement a structured, rational strategy for optimum SPE conditions in experiments.

• Readily transferable methods

Methods stored in the RapidTrace+ controller can be instantly recalled and transferred to other units with

100% confidence.

• Positive Pressure Syringe Displacement

Positive pressure displacement allows for accurate processing and improves reproducibility in flow rate sensitive methods

• Safety and Security

Enclosed working parts ensures operator safety and reduced exposure to solvents and hazardous compounds. Additionally, password protected control software limits access to methods ensuring method security.

Combined with magnetically encoded racks, run initiation is simplified and the possibility of loading an incorrect procedure is reduced.

• 8 Solvent Inlet Lines

Allows for more flexible use of solvents and provides an excellent system for method development.

• Segregated Waste Lines

Four segregated output lines allow for easy separation of aqueous, organic and biological waste. Useful to simplify hazardous waste handling and reduce disposal costs.

• Simple and Reliable

Simple, rugged design with a minimum of moving parts and easily accessible consumables allows for a robust system with minimum “down-time”.

4

Instruments


Technical Specifications

Column sizes

• 1, 3 or 6 mL

Sample size

• 0.1 mL to 14 mL

C50000 – RapidTrace+ Workstation 3 mL

(0.1 to 40 mL with upgrade to new rack sensor)

0.1 mL to 40 mL

C125713 – RapidTrace+ Workstation 6 mL

Syringe pump

• Load Volume 0.1 mL to 5.8 mL

Flow rates

• 0.1 mL/min to 42 mL/min (0.006 mL/sec to 0.7 mL/sec)

Liquid Handling Accuracy

• Volumes 3.0 mL ± 1%

Mixing Capability Cycle

• Maximum mixing volume of 5.0 mL in mixer

Liquid Sensing

• Volumes of 1.2 mL or larger

Solvent inlets

• 8 reagent inlets, individually configurable aspirate/dispense rates

Waste outlets

• 4 separate

Gas inlet (optional)

• 1-gas inlet, 2.8 bar maximum

Connections

• RS232 Input: Connects the module to the controller

RS232 Output: Used when multiple modules are connected

Dimensions

• 10 cm x 55 cm x 60 cm (W x D x H)

Weight

• 14,5 kg

Power Requirements

• 50–60 Hz, 100–240 VAC ±10%

Magnetically Encoded Racks

Magnetically Encoded Racks enable the user to designate a specific method to a rack.

When using magnetically encoded racks, the method does not have to be initiated via the controller software, because the module reads the magnets and automatically knows which method to run. The module runs the number of samples corresponding to the number of sample test tubes placed in the rack.

This means that software control of the system can be bypassed and methods will not be altered during routine operation. Labs around the world find this useful in production mode.

Compliance validation

Each unit includes a Validation Manual that allows operators to walk through the validation process of their RapidTrace+.

This ensures that the instrument provides the consistently clean extracts required for today’s sophisticated analytical instruments and regulatory bodies. The Validation Plan defines the methods and the documentation utilized in the development, execution and reporting of a validation for a

RapidTrace+. It addresses installation, operation and performance qualification. This comprehensive document enables operators to meet GLP, GMP and GOP requiremets.

Increase throughput by controlling up to 10 RapidTrace+ systems with single computer using the intuitive method development software.

Instruments

5


Accessories

Related Products

Racks

There are several racks available, utilizing the unique magnetic encoding system:

• 13 x 100 mm fraction sample

12 x 75 mm fraction tube







9 position chilled rack

• 40 mL sample flash (5 position)


(10 position)

Vial Adapters

These adapters are designed for use with the 13 x 100 mm sample tube with

12 x 75 mm fraction tubes, to collect the fraction directly into a vial rather than a test tube. Transferring the sample from a test tube to a vial takes additional time and can result in a loss of some of the sample. There are two adapters available:

• 11 mm diameter vial

(standard 1.5mL GC vial)

• 4 mL vial (threaded HPLC vial)

SPE Solutions

Biotage manufactures a wide range of products to ensure options based on the degree of cleanliness required and the cost effectiveness demanded by the analysis. Solid Phase Extraction,

Supported Liquid Extraction and Protein

Precipitation techniques are uniquely supported by consumables, automation and accessories. Your Biotage representative can help you choose the best solution for your needs.

O-Rings

The O-Rings used on the RapidTrace

+ are subject to wear and will need to be replaced occasionally depending on usage:

• 6 mL SPE O-Ring, CHEMRAZ TM

• 3 mL SPE O-Ring, CHEMRAZ

• 1 mL SPE O-Ring, CHEMRAZ

• Cannula SPE O-Ring, CHEMRAZ

• Waste Station O-Ring, CHEMRAZ

Test Tubes

Disposable borosilicate glass test tubes for RapidTrace

+

. These tubes are also compatible with TurboVap LV

Evaporator for further downstream processing.

• 12 x 75 mm

• 13 x 100 mm

• 16 x 100 mm

Solvent Evaporation

Biotage offers the largest range of solvent evaporation systems designed for faster evaporation of volatile and high-boiling solvents and sample transfer minimization. Utilizing patented vortex shearing technology, rotation, controlled heating or vacume technology, Biotage will automate your process. Ask your representative about:

• TurboVap

® systems

• SPE Dry TM

• V-10

6

Accessories

RevisedPages.qxp:Brochure 19/6/13 16:45 Page 7

Ordering Information

RapidTrace

®

+

Product

RapidTrace+ Systems

RapidTrace+ Workstation,

1 mL and 3 mL (10 columns)

RapidTrace+ Workstation, 6 mL (5 columns)

3 mL and 1 mL only, with appropriate plunger

(see accessories)

RapidTrace Start-Up Kit w/ Software

RapidTrace Notebook Controller

Quantity Part Number

1

1

1

1

C50000

C125713

C52006

C52689

Accessories

Product

Racks (Each RapidTrace+ requires one rack)

13x100 mm sample tube

12x75 mm fraction tube







13x100 mm sample tube 12x75 mm fraction tube (9 Position Chilled Rack)

40 mL sample flask (5 position)

16x100 fraction tube (10 position)

Magnet kit for encoding racks

Quantity

1 rack

5 racks

1 rack

1 rack

1 rack

1 rack

10 pack

Part Number

C50974

C50976

C58309

C56786

C56536

C133968

C54405

Product

Vial Adapters

11 mm Vial Adapter

4 mL Vial Adapter

Test Tubes

12 x 75 mm Test Tubes, uncapped



O-Rings

6 mL SPE O-Ring, CHEMRAZ



Cannula O-Ring, CHEMRAZ

Waste Station O-Ring, CHEMRAZ

(3 in a Waste Station)

Quantity Part Number

10 adapters C55091

10 adapters C55827

1,000/case C44651

1,000/case C40707

1,000/case C40708

1 O-Ring

1 O-Ring

1 O-Ring

1 O-Ring

1 O-Ring

C46643

C45096

C60969

C45096

C58526

Miscellaneous

6 mL Column Plunger

3 mL Column Plunger

1 mL Column Plunger

3 mL SPE Gel Adapter Kit

1 mL SPE Gel Adapter Kit

1 mL Adapter Sleeves

Tubing Weight Kit for Reagent Lines

Check Valve Kit for Manifold Reagent Lines

1

1

1

1

1 kit of 11

8 weights

8 valves

C69983

C52231

C52229

C58728

C58759

C59807

C50973

C55076

The Advantages of Upgrading Your Current RapidTrace

A broader range of SPE columns

Increased bed height capability enables the use of the most industry standard columns, including the entire Biotage ISOLUTE and

EVOLUTE range of 1, 3 and 6 mL columns allowing for a more flexible approach to sample volume.

Software upgrade 2.1

Customer installable software and firmware.

Note: 6 mL columns are only compatible with the 6 mL model, see below for upgrade information.

Part Number – C133966

Run 40 mL sample volume

A new 5 position rack for 40 mL scintillation vials for processing sample volumes up to

40 mL.

40 mL rack upgrade with sensor

Service visit required to install and upgrade the instrument to run the new 40 mL sample rack. Kit includes software and one rack.


Run 6 mL columns

The 5 position turret for 6 mL columns is updated for easier column loading/ unloading. Additionally, adapters enable the use of up to 5 x 1 mL or 3 mL columns in the 6 mL turret.

6 mL Turret upgrade kit

Service visit required to upgrade existing

RapidTrace 3 mL instruments with the new 6 mL compatible turret. Kit includes software and adapters for 1 mL and 3 mL columns.


NORTH AMERICA 1 800 446 4752 EUROPE +46 18 56 57 10 JAPAN +81 3 5627 3123

NORTH AMERICA 1 800 446 4752 EUROPE +46 18 56 57 10 JAPAN +81 3 5627 3123

TurboVap® LV

Evaporation

Biotage

SPE Dry 96 & 96 Dual

Sample Concentrator System

Biotage

SPE Dry 96

Sample Concentrator System

Biotage

SPE Dry 96 Dry Dual

Sample Concentrator System

Evaporation Brochure

TurboVap

Evaporators and Concentrators

TurboVap

Product Family

Solvent Evaporation

Fast, Reliable and Affordable

Fast, Automated and Safe Drying of Solvents

The Widest Range of Evaporation Solutions Available

CONTENTS

Biotage Evaporation System Selection Guide

TurboVap

Technology

TurboVap

Family

TurboVap

II

TurboVap

LV

TurboVap

500

TurboVap

96

Biotage

SPE Dry

Biotage

V-10 Evaporator

Accessories

Related Products

TurboVap

LV – Tubes and Racks Chart

Ordering Information

Biotage offers the widest range of solvent evaporation systems available. This guide presents the most suitable evaporation system for your research requirements, and answers common questions regarding service, maintenance and applications.

Biotage Evaporation System Selection Guide

TurboVap

Technology

Highest Performance, Every Time

Starting with innovative engineering, TurboVap

Highly Efficient, Patented Gas

Vortex Shearing Technology

Automated Evaporation Completion

Fast, Consistent and Even Results

TurboVap

Family

Choose the Solution That’s Right for You

TurboVap

II

Volume Range 50 mL or 200 mL

TurboVap

II increases the capacity and sample throughput in laboratories, eliminates concentration bottlenecks and maintains quality and lower costs.

Applications

Evaporation Rates (180 mL solvent)

Specifications

Advantages

Applications

TurboVap

LV

Volume Range up to 60 mL

The TurboVap

LV evaporation system is an automated high speed, low volume sample concentrator. It is an efficient alternative to the inconvenient set-up, constant monitoring and long evaporation times that are characteristic of conventional techniques.

Applications

Evaporation Rates (10 mL solvent)

Specifications

Advantages

Applications

TurboVap

500

Volume Range up to 500 mL

TurboVap