Data Sheet

Data Sheet
Concentrated and Prediluted Rabbit Monoclonal Antibody
Control Number: 901-352-032415
Catalog Number:
CRM 352 A, B
PRM 352 AA
OAI 352 T60
0.1, 0.5 ml, concentrated
6.0 ml, prediluted
60 tests, prediluted
Da Vinci Green
Intended Use:
For In Vitro Diagnostic Use
MUM-1 [BC5] is a rabbit monoclonal antibody that is intended for laboratory use in
the qualitative identification of MUM-1 protein by immunohistochemistry (IHC) in
formalin-fixed paraffin-embedded (FFPE) human tissues. The clinical interpretation of
any staining or its absence should be complemented by morphological studies using
proper controls and should be evaluated within the context of the patient’s clinical
history and other diagnostic tests by a qualified pathologist.
Summary and Explanation:
Multiple myeloma oncogene-1 (MUM-1) is a 50 kDa protein encoded by the MUM-1
gene. Studies have shown IRF4 / MUM-1 is expressed in the nuclei and cytoplasm of
plasma cells and a small percentage of germinal center (GC) B cells located in the
“light zone”. This antibody labels MUM-1 protein in centrocytes and their progeny,
plasma cells, activated T cells, and a wide spectrum of hematolymphoid neoplasms
derived from these cells (1-3).
Principle of Procedure:
Antigen detection in tissues and cells is a multi-step immunohistochemical process.
The initial step binds the primary antibody to its specific epitope. After labeling the
antigen with a primary antibody, an enzyme labeled polymer is added to bind to the
primary antibody. The detection of the bound antibody is evidenced by a colorimetric
Source: Rabbit monoclonal
Species Reactivity: Human and dog
Clone: BC5
Isotype: IgG
Total Protein Concentration: ~10 mg/ml. Call for lot specific IgG concentration.
Epitope/Antigen: MUM-1 protein
Cellular Localization: Nuclear and cytoplasmic
Positive Control: Tonsil
Known Applications:
Immunohistochemistry (formalin-fixed paraffin-embedded tissues)
Supplied As: Buffer with protein carrier and preservative
Storage and Stability:
Store at 2ºC to 8ºC. Do not use after expiration date printed on vial. If reagents are
stored under conditions other than those specified in the package insert, they must be
verified by the user. Diluted reagents should be used promptly; any remaining reagent
should be stored at 2ºC to 8ºC.
Protocol Recommendations (manual use):
Peroxide Block: Block for 5 minutes with Biocare's Peroxidazed 1.
Pretreatment Solution (recommended): Reveal
Pretreatment Protocol:
Heat Retrieval Method:
Retrieve sections under pressure using Biocare’s Decloaking Chamber, followed by a
wash in distilled water; alternatively, steam tissue sections for 45-60 minutes. Allow
solution to cool for 10 minutes then wash in distilled water.
Protein Block (Optional): Incubate for 5-10 minutes at RT with Biocare's Background
Primary Antibody: Incubate for 30 minutes at RT.
Probe: N/A
Protocol Recommendations (manual use) Cont'd:
Polymer: Incubate for 30 minutes at RT with a secondary-conjugated polymer.
Incubate for 5 minutes at RT with Biocare’s DAB – OR – Incubate for 5-7 minutes at
RT with Biocare’s Warp Red.
Counterstain with hematoxylin. Rinse with deionized water. Apply Tacha's Bluing
Solution for 1 minute. Rinse with deionized water.
Technical Note:
This antibody has been standardized with Biocare's MACH 4 detection system. It can
also be used on an automated staining system and with other Biocare polymer detection
kits. Use TBS buffer for washing steps.
Protocol Recommendations (ONCORE Automated Slide Staining System):
OAI352 is intended for use with the ONCORE Automated Slide Staining System.
Refer to the ONCORE Automated Slide Staining System User Manual for specific
instructions on its use. Protocol parameters in the ONCORE Automated Slide Stainer
Protocol Editor should be programmed as follows:
Protocol Name: MUM-1 Rb
Protocol Template (Description): Rb HRP Template 1
Dewaxing (DS Option): DS2
Antigen Retrieval (AR Option): AR2, low pH; 90°C
Reagent Name, Time, Temp.: MUM-1 Rb, 30 min., 25°C
The optimum antibody dilution and protocols for a specific application can vary. These
include, but are not limited to fixation, heat-retrieval method, incubation times, tissue
section thickness and detection kit used. Due to the superior sensitivity of these unique
reagents, the recommended incubation times and titers listed are not applicable to other
detection systems, as results may vary. The data sheet recommendations and protocols
are based on exclusive use of Biocare products. Ultimately, it is the responsibility of
the investigator to determine optimal conditions. The clinical interpretation of any
positive or negative staining should be evaluated within the context of clinical
presentation, morphology and other histopathological criteria by a qualified
pathologist. The clinical interpretation of any positive or negative staining should be
complemented by morphological studies using proper positive and negative internal
and external controls as well as other diagnostic tests.
Quality Control:
Refer to CLSI Quality Standards for Design and Implementation of
Immunohistochemistry Assays; Approved Guideline-Second edition (I/LA28-A2).
CLSI Wayne, PA, USA ( 2011
1. This antibody contains less than 0.1% sodium azide. Concentrations less than 0.1%
are not reportable hazardous materials according to U.S. 29 CFR 1910.1200, OSHA
Hazard communication and EC Directive 91/155/EC. Sodium azide (NaN3) used as a
preservative is toxic if ingested. Sodium azide may react with lead and copper
plumbing to form highly explosive metal azides. Upon disposal, flush with large
volumes of water to prevent azide build-up in plumbing. (Center for Disease Control,
1976, National Institute of Occupational Safety and Health, 1976) (4)
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Concentrated and Prediluted Rabbit Monoclonal Antibody
Control Number: 901-352-032415
Precautions cont'd:
2. Specimens, before and after fixation, and all materials exposed to them should be
handled as if capable of transmitting infection and disposed of with proper precautions.
Never pipette reagents by mouth and avoid contacting the skin and mucous membranes
with reagents and specimens. If reagents or specimens come into contact with sensitive
areas, wash with copious amounts of water. (5)
3. Microbial contamination of reagents may result in an increase in nonspecific
4. Incubation times or temperatures other than those specified may give erroneous
results. The user must validate any such change.
5. Do not use reagent after the expiration date printed on the vial.
6. The SDS is available upon request and is located at
Follow the antibody specific protocol recommendations according to data sheet
If atypical results occur, contact Biocare's Technical Support at
1. Carbone A, et al. Expression pattern of MUM1/IRF4 in the spectrum of pathology
of Hodgkin's disease. Br J Haematol. 2002 May;117(2):366-72.
2. Falini B, Mason DY. Proteins encoded by genes involved in chromosomal
alterations in lymphoma and leukemia: clinical value of their detection by
immunocytochemistry. Blood. 2002 Jan 15;99(2):409-26.
3. Johnson LR, Nalesnik MA, Swerdlow SH. Impact of Epstein-Barr virus in
monomorphic B-cell posttransplant lymphoproliferative disorders: a histogenetic study.
Am J Surg Pathol. 2006 Dec;30(12):1604-12.
4. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22,
Atlanta, GA. April 30, 1976 "Decontamination of Laboratory Sink Drains to Remove
Azide Salts."
5. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory
Workers from Occupationally Acquired Infections; Approved Guideline-Fourth Edition
CLSI document M29-A4 Wayne, PA 2014.
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