User Manual SuperCycler

User Manual SuperCycler
User Manual
SuperCycler
SC-200
Revision 3.0
2011
Korea
Kyratec Life Science
IT Premier Tower 1304, 345-50
Gasan-dong Geumcheon gu
Seoul Korea, 153-803
Website: www.kyratec.com
Australia
Kyratec Pty Ltd
3/17 Dividend St Mansfield
Brisbane, Queensland
Australia, 4122
Website: www.kyratec.com
1. F o re w o r d ....................................................................................................................................................................3
2. F ea t u res ......................................................................................................................................................................4
3. G e t t i n g S ta r te d ..........................................................................................................................................................6
3. 1 R e g i o n Selec t i o n .......................................................................................................................................................6
3.2 N a v i ga t i n g t h e S o f t w a re ...........................................................................................................................................7
4. P C R P r o f i le C r ea t i o n .................................................................................................................................................8
4. 1 W i z a r d M o d e ...........................................................................................................................................................8
5. M a n u a l P r o f i le G e n e ra t i o n a n d E x e c u t i o n ..............................................................................................................1 0
5. 1 C r ea t i n g a n e w E v e n t .............................................................................................................................................1 1
5.2 S ta r t i n g a r u n ..........................................................................................................................................................1 3
6. M a n u a l B l o c k C o n t r o l .............................................................................................................................................1 5
7. C o n f i g u ra t i o n T o o ls .................................................................................................................................................1 6
7. 1 U se r A c c o u n ts .........................................................................................................................................................1 7
7.2 F i le M a n a ge r ...........................................................................................................................................................1 7
7.3 M a c h i n e Se t t i n gs ....................................................................................................................................................1 8
7.4 S o f t w a re U p d a te .....................................................................................................................................................1 9
7.5 G a m es & E x t r as .....................................................................................................................................................20
8 U se r T i p s ...................................................................................................................................................................20
9 S peci f ica t i o ns ............................................................................................................................................................22
1 0 T e r m s o f U se ...........................................................................................................................................................24
Kyratec SuperCycler User Manual v3.0
1. Foreword
C o n g r a t u la t i o ns o n p u r c h asi n g a K y r a tec S u p e r C y c ler. Y o u are n o w o n e o f a g r o w i n g n u m b e r o f h i g h l y sk il le d
c ust o m e rs w o r l d w i d e t o a d o p t t h e S u p e r C y c le r as t h e i r p re fe r re d T h e r m a l C y c l i n g E n g i n e.
O u r p h i l oso p h y h as bee n t o ge ne ra te use r f r ie n d l y ye t p o w e r f u l cycl i n g w o r k h o rse. W e t r us t y o u w i l l b e m o s t
p lease d w i t h o u r p r o d u c t h o w e ve r al w a ys w e lc o m e a n y su g gesti o ns y o u m a y h a ve t o m a k e o u r p r o d u c ts b e t te r f o r
t h e f u t u re. Fee l f r ee t o e m a i l us: su p p o r t @ k y ra tec.co m
Kyratec SuperCycler User Manual v3.0
3
2. Features
General
T h e S u p e r C y c le r is a h i g h p e r f o r m a n ce b l o c k cycl i n g syste m co n f i g u re d a n d o p t i m ise d f o r I n d us t r y sta n da r d 200 u l
i n d i v i d ua l o r str i p t u bes (do m e d o r f la t-ca p pe d) o r 96-w e l l p la tes (lo w o r h i g h ski r t) w i t h st r i p ca ps o r a d h esive f i l m
seals. I t i n c o r p o ra tes sta te o f t h e a r t elec t r o n ics, p r ecisio n q u a l i t y Fe r r o tec ™ p e l t ie r d e v ices a n d a f lex i b le use r
i n te r face.
Configuration
T h e S u p e r C y c le r u t i l ises eig h t Pe l t ie r d e v ices t o act i ve ly h ea t a n d co o l t h e b l o c k be t w ee n 4° C a n d 99° C. T h ese are
ar ra n ge d i n t o f o u r z o n es w i t h i n d e pe n de n t se nsi n g a n d co n t r o l ca pa b i l i t ies. T h is C o n f i g u ra t i o n o f p e l t ie rs a n d
se nso rs e na b les a h i g h l y l i n ea r g ra d ie n t t o b e esta b l is he d v ia a so p h is t ica te d co m p u te r co n t r o l syste m.
Linear Gradient (*Available in selected regions only)
T h e r m a l g ra d ie n t tec h n o l o g y e na b les a va r y i n g te m p e ra t u re t o be set acr oss t h e react i o n w e l ls o f t h e b l o c k.
A p p l i ca t i o ns o f t h is fea t u re i n c l u d e t h e a b i l i t y t o o p t i m i ze t h e a n neali n g te m p e ra t u re o f a n assay i n a si n g le
ex pe r i m e n t b y d e te r m i n i n g t h e te m p e ra t u re o f t h e w e l ls t h a t y iel d t h e b est resu l t. T h e S u p e r C y c le r is ca pa b le o f
ge ne ra t i n g a l i n ea r g ra d ie n t o f be t w ee n 0° C a n d 20° C acr oss t h e b l o c k. T h e so f t w a re i n te r p o la tes a n d d is p la ys t h e
te m p e ra t u re o f eac h c o l u m n o f w e l ls i n real-t i m e d u r i n g a r u n.
Thermal profile engine
T h e S u pe r C y c le r h as a p o w e r f u l t h e r m a l p r o f i le e n g i ne i m p le m e n te d w i t h i n. A p r o f i le m a y co n ta i n u p t o 1 0 0
eve n ts. E a c h ‘eve n t’ ca n be ei t h e r a h o l d a t te m p e ra t u re, p a use o r 2 t o 5 ste p cycl i n g w i t h u p t o 1 0 0 re pea ts. A n y
eve n t o r ste p ca n co n ta i n g ra d ie n t, t o u c h d o w n o r l o n g ra n ge fea t u res. A n al m os t u n l i m i te d n u m b e r o f p r o f i les m a y
b e sto re d o n t h e d e v ice f o r re-r u n n i n g. D e s p i te i ts ca pa b i l i t ies p r o f i le set u p is st ra ig h t f o r w a r d.
LongRange
T h e L o n g R a n g e fea t u re e na b les t h e t i m e o f a p a r t ic u la r cycl i n g ste p t o b e a u t o m a t ical l y i n c rease d o r d ecrease d b y a
p reset a m o u n t o ve r a speci f ie d ra n ge o f cycle re pea ts. T h e L o n g R a n g e i n c reasi n g t i m e m o d e fea t u re is o f te n use d t o
p r o v i de g ra d ua l l y ex te n de d t i m es f o r e n z y m a t ic p o l y m e r i za t i o n o f l o n ge r p r o d u c ts d u r i n g la te r cycle re pea ts o f a n
a m p l i f ica t i o n reac t i o n. I t is m o s t use f u l f o r ta r ge ts l o n ge r t h a n ab o u t 1 k b. L o n g R a n ge d ec reasi n g t i m e m o d e ca n be
use d t o sh o r te n t h e t o ta l r u n t i m e. D u r i n g la te r cycles t h e m a j o r i t y o f e n z y m a t ic p o l y m e r i za t i o n is o cc u r r i n g o n
p re v i o usly ge ne ra te d a m p l ic o ns w h i c h are sh o r te r i n le n g t h t h a n t h e o r i g i n a l te m p la te. S h o r te r h o l d t i m es are o f te n
su f f ic ie n t f o r p o l y m e r i za t i o n o f t h ese sh o r te r le n g t h p r o d u c ts.
Kyratec SuperCycler User Manual v3.0
4
TouchDown
T h e T o u c h d o w n f u n c t i o n e na b les t h e te m p e ra t u re o f a ste p t o be a u t o m a t i ca ll y d ecrease d b y a p rese t a m o u n t o ve r a
ra n ge o f successive cycle re pea ts. I ts p r i m a r y use is as a m e c h a n is m t o m i n i m ise p r i m e r d i m e r ar t i fac ts b y g ra d ua l l y
d e creasi n g t h e a n neali n g te m p e ra t u re d u r i n g i n i t ia l cycle re pea ts o f a n a m p l i f i ca t i o n p r o f i le.
Pause
T h e Pa use f u n c t i o n all o ws t h e user t o p a use t h e p r o f i le a t a n y n u m b e r o f p re p r o g ra m m e d p o i n ts w i t h a n a u d i b le
b ee p. T h e cu r re n t set te m p e ra t u re is h e l d i n d e f i n i te l y u n t i l t h e C o n t i n u e b u t t o n is p resse d. H o l ds are use f u l i f t h e re
is a n ee d t o re m o ve t u b es o r a d d reage n ts a t a p a r t ic u la r p o i n t i n a r u n.
Manual Control
T h e S u p e r C y c le r also su p p o r ts M a n u a l M o d e co n t r o l. T h is m o d e e na b les t h e use r t o set t h e b l o c k t o a speci f ic
te m p e ra t u re q u i c k l y w i t h o u t crea t i n g a t h e r m a l p r o f i le. T h is f u n c t i o n is use f u l f o r i n c u b a t i n g reac t i o ns suc h as
D N A d i gesti o n o r l i ga t i o n. M a n u a l M o d e also su p p o r ts t h e r m a l g ra d ie n t.
Evaporation control Heated Lid
T h e S u pe r C y c le r e m p l o ys a h ea te d l i d d esig n t o k ee p t h e air co n ta i n e d w i t h i n t h e t u b e h o t te r t h a n t h e reac t i o n
v o l u m e. T h is ca uses a n y eva p o ra t i o n t o co n d e nse bac k i n t o t h e co o le r reac t i o n l i q u i d t h e re b y eli m i n a t i n g t h e n ee d
f o r o i l o r w a x co n d e nsa t i o n o ve r lay.
User Interface
T h e S u p e r C y c le r h as a 7” co l o u r t o u c h scree n g i v i n g a v i b ra n t a n d f le x i b le m e a ns o f r u n set u p a n d m o n i t o r i n g. T h is
i n te r n a l g ra p h i ca l user i n te r face co n t r o l le r p r o v i des a p o w e r f u l ye t i n t u i t i ve a n d easy t o use i n te r face. A l l
S u pe r C y c le rs h a ve a U S B p o r t a n d (soo n t o be release d) W i n d o w s base d P C so f t w a re. T h is so f t w a re o p e ns t h e use r
u p t o easier p r o f i le set u p, sha r i n g o f f i les, a n d p os t r u n exa m i n a t i o n o f a n y te m p e ra t u re d a ta.
N e w so f t w a re fea t u res a n d u p d a tes are re g u la r l y i n c o r p o ra te d a n d ava ila b le f o r ei t h e r syste m as f r ee u p g ra des v ia
w e b d o w n l oa d.
Kyratec SuperCycler User Manual v3.0
5
3. Getting Started
P l u g t h e u n i t i n t o a p o w e r o u t le t a n d p o w e r t h e u n i t o n w i t h t h e s w i tc h l o ca te d o n t h e rear o f t h e u n i t. D o n o t
w o r r y a b o u t select i n g t h e co r rec t p o w e r v o l ta ge f o r y o u r co u n t ry, T h e S u pe r C y c le r is d esig n e d t o o p e ra te i n a n y
c o u n t r y i n t h e w o r l d w i t h n o c ha n ges. T h e u n i t is also d esig ne d t o be b e resista n t t o p o w e r su r ges a n d d r o p-o u ts
t h a t o cc u r i n c o u n t r ies w i t h less rel ia b le p o w e r g r i ds.
O n p o w e r u p t h e S u p e r C y c le r w i l l d is p la y t h e l o g o a n d ta ke a fe w m o m e n ts t o i n i t ia te t h e so f t w a re.
3.1 Region Selection
W h e n t h e u n i t is p o w e re d o n f o r f i rst t i m e ever t h e re g i o n select i o n a n d E n d U se r L i ce n ce A g r ee m e n t (E U L A) w i l l
b e d is p laye d. T h e o p e ra t o r is re q u i re d t o select t h e i r c o r rec t re g i o n o f p u r c hase a n d o pe ra t i o n, rea d t h e te r ms o f use
a n d c hec k t h e 'agree' b o x t h e n p ress t h e g ree n t i c k t o co n t i n u e. N o t e t h a t availa b le fea t u res va r y b e t w ee n d i f fe re n t
re g i o ns. T h e fea t u res t h a t are availa b le f o r y o u r use are al w a ys as speci f ie d t o y o u be f o re y o u r p u r c h ase o f t h e
e q u i p m e n t.
I f t h e i ns t r u m e n t is m o ve d t o a d i f fe re n t re g i o n o f o pe ra t i o n a t a la te r d a te t h e n t h e re g i o n co n f i g u r a t i o n scree n m a y
b e reac he d t h r o u g h t h e 'settings' ic o n u n d e r t h e co n f i g u r a t i o n m e n u.
A co p y o f t h e T e r m s o f use m a y also b e f o u n d a t t h e e n d o f t h is m a n u a l.
Kyratec SuperCycler User Manual v3.0
6
3.2 Navigating the Software
A f t e r p o w e r u p t h e u n i t d e fa u l ts t o t h e m a i n scree n f r o m w h e re all fea t u res m a y b e accesse d. Y o u m a y re t u r n t o t h is
scree n at a n y t i m e w i t h o u t i n te r r u p t i n g a r u n b y p ressi n g t h e H o m e l o g o i n t h e b o t t o m le f t h a n d co r n e r f o r t h e
scree n.
T h e 4 scree n ic o ns p r o v i de access t h e f o l l o w i n g fea t u res:
C O N FI G UR ATI O N – T a k es y o u t o t h e co n f i g u ra t i o n p a ge all o w i n g o f m a c h i n e o p t i o ns.
PC R RU N – Is use d t o l oa d o r crea te a n n e w P C R t h e r m a l p r o f i le a n d i n i t ia te i ts execu t i o n.
M A NU A L C O N T R O L – A l l o w s i m m e d ia te m a n u a l set t i n g o f b l o c k te m p e ra t u re.
I N FO R M AT I O N – D i s p la ys a ge ne ra l i n f o r m a t i o n scree n w h i c h i n c l u des suc h i n f o r m a t i o n as ser ial n u m b er,
so f t w a re a n d h a r d w a re ve rsi o n n u m b e rs. A n o n-scree n user m a n u a l is accessib le f r o m u n d e r t h is p a ge m e n u
T h e b a r a t t h e b o t t o m o f t h e scree n d is p la ys use f u l i n f o r m a t i o n ab o u t t h e d e v ice a n d r u n sta t us. T h e f i rst b o x
d is p lays t h e c u r re n t act iv i ty, i t d is p lays suc h i n f o r m a t i o n as 'i d le', 'ra m p i n g t o te m p e ra t u re' a n d ' h o l d – x x' w i t h a
c o u n t d o w n o f t h e re m a i n i n g h o l d t i m e a t t h e p a r t i c u la r step. T h e seco n d b o x d is p la ys t h e E l a pse d r u n t i m e a n d t h e
last b o x t h e t o ta l E s t i m a te d r u n t i m e. T h e act ual r u n t i m e m a y slig h t l y d i f fe r f r o m t h e esti m a te d t i m e d e pe n d i n g o n
fac t o rs suc h as t h e a m b ie n t air te m p e ra t u re, h u m i d i t y a n d t h e t y p e o f p lastic w a re a n d l i q u i d v o l u m es o n t h e b l o c k.
T h e c u r re n t use r n a m e a n d p r o f i le p ic t u re is also d is p laye d as w e l l as t h e c u r re n t t i m e a n d d a te.
T h r o u g h o u t t h e so f t w a re h e l p is availa b le i n n u m e r o us l o ca t i o ns b y p ressi n g t h e q uest i o n m a r k l o g o i f ava ila b le.
Kyratec SuperCycler User Manual v3.0
7
4. PC R Profile Creation
F r o m t h e ' h o m e' scree n t h e ' P C R R u n ' ic o n w i l l b r i n g y o u t o t h e ex pe r i m e n t p a ge. H e r e y o u w i l l h a ve 4 o p t i o ns,
y o u ca n l oa d a n exist i n g ex pe r i m e n t f r o m ei t h e r t h e i n te r n a l d r i ve o r a n ex te r na l U S B d r i ve, o r crea te a n e w t h e r m a l
p r o f i le usi n g ei t h e r t h e W i z a r d o r t h e m a n u a l b u i l der.
W h e n l oa d i n g f i les f r o m t h e ' I n te r n a l D r i ve' y o u o n l y h a ve access t o t h e f i les u n d e r t h e d i rec t o r y o f t h e user t h a t
y o u are c u r re n t l y l o g ge d i n as. I f y o u are n o t l o g ge d i n as a re g is tere d use r y o u r f i le access w i l l b e u n d e r t h e ' D e fa u l t'
use r d i rec t o ry.
4.1 Wizard Mode
T h e S u p e r C y c le r so f t w a re i n c l u d es a W i z a r d u t i l i t y w h i c h e na b les t h e user t o co n f i g u re easy t o m o d e ra te
co m p le x i t y p r o f i les i n j ust m o m e n ts. T o act iva te select ' W i za r d' f r o m t h e P C R sta r t u p scree n.
Kyratec SuperCycler User Manual v3.0
8
T h e W i z a r d scree n d is p la ys eac h o f t h e ste ps w h i c h o cc u r i n a t y p i ca l ra n ge o f p r o f i les. A t a m i n i m u m t h e re are 3
ste ps, P r e hea t a n d t h e n d e na t u ra t i o n a n d a neal w h i c h are cycle d. Y o u m a y e na b le u p t o a f u r t h e r 3 ste ps (c D N A,
E x t e nsi o n & p os t r u n h o l d) b y cl ic k i n g o n t h e c o r resp o n d i n g c hec k b o xes. E a c h o f t h e ste ps w i l l be exec u te d i n t h e
o r de r t h e y are sh o w n ar ra n ge d d o w n t h e scree n w i t h t h e D e n a t u r a t i o n, A n e a l a n d E x t e nsi o n (if e na b le d) ste ps
b e i n g cycle d. A f t e r t h e a m p l i f ica t i o n r u n h as bee n co m p le te d t h e te m p e ra t u re m a y be h e l d at a d e f i n e d te m p e ra t u re
u n t i l t h e use r d isa b les t h e u n i t. T o a d j ust a n y o f t h e p a ra m e te rs cl ic k o n t h e c o r resp o n d i n g t i m e o r te m p e ra t u re
b o xes a n d a k e y pa d w i l l be p r ese n te d all o w i n g e n te r i n g o f n e w n u m b e rs. T h e n u m b e r o f cycles ca n also be a d j uste d
b e t w ee n 1 (sin g le exec u t i o n, n o l o o ps) a n d 99 cycles.
W h e n t h e user is h a p p y w i t h t h e p r o f i le p a ra m e te rs j us t c l ic k o n t h e t i c k. A r u n rev ie w scree n w i l l n o w be sh o w n
w i t h a te x t d escr i p t i o n o f t h e p r o f i le ste ps. C l i c k o n O K t o co m m e n ce r u n execu t i o n o r ' E d i t P r o f i le' i f y o u re q u i re
t o f u r t h e r m o d i f y t h e w i za r d crea te d p r o f i le. T h e 'Save & R u n ' I c o n all o w s y o u t o save t h e p r o f i le t h e n r u n i t.
O n ce t h e p r o f i le is co n f i r m e d, i f t h e 'Use Top Heater' c hec k b o x o p t i o n is selec te d (defa u l t) t h e n y o u w i l l be p r o m p te d
Kyratec SuperCycler User Manual v3.0
9
w i t h a scree n ask i n g i f y o u w is h t o w a i t f o r t h e t o p h ea te r t o reac h ta r ge t te m p e ra t u re b e f o re co m m e n c i n g t h e r u n.
I f y o u select t o w a i t f o r t h e t o p h ea te r t o ac h ie ve te m p e ra t u re a w a i t i n g scree n w i l l be d is p laye d. Y o u m a y ski p t h e
w a i t i n g ste p at a n y t i m e i f y o u w is h t o co m m e n ce t h e r u n i m m e d ia te ly. D e p e n d i n g o n t h e set te m p e ra t u re o f t h e
T o p H e a te r i t w i l l ta ke u p t o seve ral m i n u tes t o p re h ea t t o t h is te m p e ra t u re.
W h e n t h e r u n co m m e n ces t h e so f t w a re w i l l a u t o m a t i cal l y p r o cee d t o t h e ' R u n ' scree n. Y o u are n o t ab le t o m o d i f y
t h e p r o f i le o n ce a r u n h as co m m e n ce d b u t y o u m a y save o r e d i t i t a f te r i t co m p le tes o r is te r m i n a te d.
F r o m t h e r u n rev ie w scree n y o u m a y also c hec k t h e o p t i o n 'r u n i n v i r t u a l m o d e'. T h is w i l l m a k e t h e S C si m u la te t h e
r u n ra t h e r t h a n p e r f o r m i n g t h e act ua l t h e r m a l ste ps o n t h e b l o c k. T h is ca n be use f u l i f y o u w is h t o c hec k
co m p l i ca te d r u ns p r i o r t o p e r f o r m i n g t h e m.
5. Manual Profile Generation and Execution
T h e m a n u a l p r o f i le e d i t o r is ev o ke d f r o m t h e P C R E x p e r i m e n t scree n. T h is scree n d is p la ys t h e c u r re n t ex pe r i m e n t
p r o f i le i n a 't ree l is t' f o r m a t. W h e n y o u select a n eve n t f r o m t h e t ree l ist, t h e co r resp o n d i n g t h e r m a l p r o f i le f o r t h a t
eve n t w i l l b e d is p laye d o n t h e r i g h t h a n d si de o f t h e scree n. N o t e t h a t i t is o n l y t h e f i rst fe w m i n u tes o f t h a t eve n t
w h i c h are d is p laye d g ra p h i cal ly. W h e n a n eve n t is selecte d a su b m e n u w i l l be d is p laye d g i v i n g f u r t h e r o p t i o ns f o r
t h is eve n t. T h e selec te d eve n t m a y be m o ve d u p o r d o w n i n o r de r w i t h i n t h e p r o f i le eve n ts, d e le te d, o r e d i te d.
T h e p r o f i le ge ne ra t i o n scree n co n ta i ns 8 ic o n b u t t o ns w i t h t h e f o l l o w i n g f u n c t i o n a l i t y:
H E LP – T h is b u t t o n w i l l d is p lay t h e o n scree n user m a n u a l w i t h t h e releva n t p a ges selec te d
STA RT RU N – T h is b u t t o n is use d t o act i va te a r u n. P ressi n g t h is b u t t o n w i l l sh o w a p r o f i le rev ie w be f o re
co m m e n c i n g a r u n
Kyratec SuperCycler User Manual v3.0
10
A D D EVE N T – E v o kes t h e A d d S te p w i za r d t o b u i l d a n e w p r o f i le step.
A D D N O T ES – A l l o w s t h e o pe ra t o r t o w r i te a n d save a n y n o tes alo n g w i t h t h e r u n.
SAVE – A l l o w s a g ive n p r o f i le t o b e save d t o i n te r na l f las h m e m o r y d is k. T h e u n i t h as ve r y la r ge i n te r na l m e m o r y
all o w i n g v i r t u a l l y u n l i m i te d p r o f i les t o be save d (in t h e o r de r o f te ns o f t h o usa n ds). Sa vi n g a p r o f i le also st o res t h e
d e v ice o p t i o ns co n f i g u ra b le u n d e r t h e set t i n gs m e n u, ie t o p h ea te r te m p e ra t u re a n d o t h e r f u n c t i o n a l i ty. Y o u m a y
e n te r a n y val i d f i le n a m e f o r savi n g. P r o f i le f i le w i l l be save d t o t h e c u r re n t user d i rec t o ry, o r t h e D e f a u l t d i rec t o r y i f
n o use r is selecte d.
L O A D – L o a ds a p r esave d p r o f i le f r o m t h e i n te r n a l f las h m e m o ry. T h e S u pe r C y c le r d is p lays a l is t o f t h e save d f i les
o n t h e u n i t i n t h e d i rec t o r y o f t h e cu r re n t l y selecte d user. I f n o user is selecte d i t w i l l sh o w t h e f i les save d i n t h e
d e fa u l t user d i rec t o ry. T o access a d i f fe re n t users f i les y o u n ee d t o l o g i n as t h a t user.
C LE AR PROFI LE – w i l l erase t h e cu r re n t p r o f i le. U se r co n f i r m a t i o n is re q ueste d be f o re execu t i o n.
SE T TI N G S – w i l l d is p la y t h e syste m set t i n gs all o w i n g c h a n ges t o t o p h ea te r f u n c t i o n a n d te m p e ra t u re a n d o t h e r
f u n c t i o ns.
5.1 Creating a new Event
T o crea te a n e w eve n t select t h e ' A d d E v e n t' ic o n i n t h e m a n u a l p r o f i le e d i t o r. T h e f i rst scree n o f t h e A d d E v e n t
W i z a r d w h i c h w i l l be d is p laye d le ts t h e user selec t t h e t y p e o f eve n t.
Y o u m a y c h o ose f r o m 4 eve n t t y pes.
Kyratec SuperCycler User Manual v3.0
11
H O L D – I s a si m p le h o l d a t g ive n te m p e ra t u re f o r a set t i m e. I t m a y be p e r f o r m e d ei t h e r w i t h o r w i t h o u t a
g ra d ie n t acr oss t h e b l o c k.
T h e h o l d scree n all o w s t h e o pe ra t o r t o set t h e h o l d t i m e a n d te m p e ra t u re. I f t h e G r a d ie n t * o p t i o n is selec te d b o t h
t h e le f t a n d r i g h t te m p e ra t u res m a y be set sepa ra te ly a n d t h e p re d ic te d w e l l te m p e ra t u res ca n be sh o w n b y cl ic k i n g
t h e P re d ic te d T e m p s l i n k.
R A M P – A l l o w s t h e b l o c k te m p e ra t u re t o be ra m p e d ei t h e r u p o r d o w n b e t w ee n t w o set te m p e ra t u res o ve r a p rese t
le n g t h o f t i m e. T h e b l o c k ta r ge t te m p e ra t u re is u p d a te d eve r y seco n d a n d w i t h a 0.025 d e g ree m i n i m u m ste p
reso l u t i o n. I t m a y be p e r f o r m e d ei t h e r w i t h o r w i t h o u t a g ra d ie n t acr oss t h e b l o c k.
T h e ra m p scree n sh o ws t h e i n i t ia l a n d f i na l ra m p te m p e ra t u res. I f t h e G r a d ie n t * o p t i o n is selec te d b o t h t h e le f t a n d
r i g h t te m p e ra t u res m a y b e set sepa ra te l y a n d t h e p re d ic te d w e l l te m p e ra t u res ca n b e sh o w n b y cl ic k i n g t h e
P re d ic te d T e m p s l i n k.
PAUSE – w i l l h o l d t h e te m p e ra t u re a t t h e last set p o i n t a n d p a use t h e r u n w i t h a n au d i b le w a r n i n g. T h is is use f u l i f
t h e o p e ra t o r n ee ds t o p e r f o r m a ce r ta i n act i o n o f t h e sa m p les at a g i ve n p o i n t i n t h e r u n. T h e o pe ra t o r cl ic ks o k
w h e n co m p le te a n d t h e r u n w i l l co n t i n u e w i t h t h e n e x t eve n t.
C YC LI N G x STEP – w i l l p r o cee d t o ge ne ra te a m u l t i ste p cycl i n g eve n t. T h e scr o l l b u t t o n t o t h e r i g h t all o w s y o u
t o c ha n ge t h e n u m b e r o f ste ps b e t w ee n 2 a n d 5 f o r t h e cycl i n g eve n t.
W i t h i n t h e cyc l i n g co n f i g u ra t i o n scree ns t h e re are n u m e r o us o p t i o ns. O n c e aga i n I f t h e G r a d ie n t * o p t i o n is selec te d
b o t h t h e le f t a n d r i g h t te m p e ra t u res m a y b e set sepa ra te ly a n d t h e p re d ic te d w e l l te m p e ra t u res ca n b e sh o w n b y
cl ic k i n g t h e P re d ic te d T e m p s l i n k. T h e re are also o p t i o ns f o r T o u c h d o w n a n d L o n g ra n ge fea t u res. Se lec t i n g t h ese
c hec k b o xes w i l l d is p lay ex t ra b u t t o ns t o co n f i g u re t h ese fea t u res.
Touchdown – all o ws f o r t h e te m p e ra t u re o f a g i ve n ste p w i t h i n a cycl i n g eve n t t o be p r o g ressively i n c rease d o r
d e crease d f r o m a n i n i t ia l va l ue t o a f i na l val ue o ve r a d e f i n e d n u m b e r o f ste ps.
LongRange – all o ws t h e h o l d t i m e o f a g i ve n ste p w i t h i n a cycl i n g eve n t t o be p r o g ressively i n c rease d o r d ecrease d
f r o m a n i n i t ia l val ue t o a f i n a l val ue o ve r a d e f i ne d n u m b e r o f ste ps.
A n y o r all o f t h e ste ps w i t h i n a cycl i n g eve n t m a y use t h ese fea t u res. T h e f o l l o w i n g p i c t u re d is p la ys t h e sa me scree n
w i t h all o p t i o ns (Gradient*, Touchdown & Long Range) e na b le d.
Kyratec SuperCycler User Manual v3.0
12
T h e 'Next' b u t t o n w i l l p r o cee d t h r o u g h eac h o f t h e p a ra m e te r scree ns f o r eac h ste p co n ta i ne d w i t h i n t h e cycl i n g.
A f t e r t h e p a ra m e te rs f o r eac h ste p are co n f i g u re d t h e o p e ra t o r is p r o m p te d t o e n te r t h e n u m b e r o f t i m es t o re pea t
t h e cycle.
* Note - Gradient function not available in all regions or in all models.
5.2 Starting a run
A c t i va t i n g t h e Start Run b u t t o n w i l l sh o w a p r o f i le rev ie w p a ge w h i c h all o ws t h e o p e ra t o r t o c hec k o ve r t h e p r o f i le
(disp laye d i n te x t f o r m a t) be f o re co n f i r m i n g co m m e n ce m e n t. T h e p r o f i le re vie w w i l l also co n ta i n ge ne ral r u n
o p t i o ns re ga r d i n g t h e T o p H e a te r eva p o ra t i o n co n t r o l a n d ac t i o ns o n r u n co m p le t i o n. T h ese are co n f i g u ra b le u n d e r
t h e co n f i g u ra t i o n m e n u. Y o u h a ve t h e o p t i o n t o p e r f o r m t h e r u n i n v i r t ua l m o d e, t h is w i l l si m u la te t h e r u n w i t h o u t
act ua l ly t h e r m a l l y va r y i n g t h e b l o c k.
O n ce t h e p r o f i le is co n f i r m e d, I f t h e 'Use Top Heater' c hec k b o x o p t i o n is selecte d (defa u l t) u n d e r t h e set u p m e n u
t h e n y o u w i l l b e p r o m p te d w i t h a scree n as k i n g i f y o u w is h t o w a i t f o r t h e t o p h ea te r t o reac h ta r ge t te m p e ra t u re
b e f o re co m m e n c i n g t h e r u n.
I f y o u select t o w a i t f o r t h e t o p h ea te r t o ac h ie ve te m p e ra t u re a w a i t i n g scree n w i l l be d is p laye d. Y o u m a y ski p t h e
w a i t i n g ste p at a n y t i m e i f y o u w is h t o co m m e n ce t h e r u n i m m e d ia te ly. D e p e n d i n g o n t h e set te m p e ra t u re o f t h e
T o p H e a te r i t w i l l ta ke u p t o seve ral m i n u tes t o p re h ea t t o t h is te m p e ra t u re.
O n ce t h e r u n co m m e n ces t h e so f t w a re w i l l a u t o m a t i ca ll y p r o cee d t o t h e ' R u n ' scree n. Y o u are n o t a b le t o m o d i f y
t h e p r o f i le o n ce a r u n h as co m m e n ce d.
Kyratec SuperCycler User Manual v3.0
13
O n r u n co m p le t i o n t h e p os t r u n re p o r t scree n w i l l sh o w a n d i f e na b le d a n au d i b le alar m w i l l so u n d. T h e ala r m m a y
b e m u t e d b y p ressi n g t h e m u t e ala r m b u t t o n. T h e p os t r u n re p o r t ca n b e save d as a te x t f i le t o a U S B m e m o r y stic k
f o r i n c l usi o n i n t o la b b o o ks etc. N o t e t h a t p os t r u n re p o r ts are n o t save d t o i n te r n a l m e m o ry.
Kyratec SuperCycler User Manual v3.0
14
6. Manual Block Control
T h e m a n u a l m o d e scree n all o ws i m m e d ia te m a n u a l set t i n g o f t h e b l o c k te m p e ra t u re. T o ac t iva te t h is m o d e si m p l y
p ress t h e b l ue 'sta r t' b u t t o n. T e r m i n a t i n g t h e m o d e is ac h ieve d b y p ressi n g t h e re d 'st o p' b u t t o n. T o P r o g ra m t h e set
te m p e ra t u re p ress t h e te m p e ra t u re b u t t o n t o t h e le f t o f t h e g ra p h a n d e n te r t h e d esire d val ue. Y o u m a y p r o g ra m t h e
te m p e ra t u re set p o i n t t o 0. 1 d e g ree C reso l u t i o n. I f t h e ' G r a d ie n t' * c hec k b o x is c hec ke d t h e ' Te m p R i g h t' b u t t o n
a p pears a n d y o u are a b le t o set d i f fe re n t te m p e ra t u res f o r b o t h t h e le f t a n d r i g h t si des o f t h e b l o c k w i t h a r o u g h l y
l i nea r t h e r m a l g ra d ie n t p rese n te d b e t w ee n t h e t w o o u te r w e l l si des. T o e na b le t h e T o p H e a te r eva p o ra t i o n co n t r o l
cl ic k t h e ' T o p H e a t' c h ec k b o x. T h e ta r ge t te m p e ra t u re o f t h e t o p h ea te r is co n f i g u re d u n d e r t h e C o n f i g u ra t i o n →
Se t t i n gs m e n u. N o t e t h e t o p h ea te r w i l l ta ke a fe w m i n u tes t o reac h i ts o p e ra t i n g te m p e ra t u re. T h e le f t si de o f t h e
scree n d is p lays t h e te m p e ra t u res o f eac h o f t h e 4 z o n es. O t h e r i n f o r m a t i o n also d is p laye d is t h e eve n t, ste p a n d cycle
n u m b e rs f o r w h e n a p r o f i le is be i n g execu te d. A t t h e b o t t o m o f t h is d is p lay b o x is t h e t o p h ea te r (T H) a n d i n te r na l
h ea tsi n k (H S) te m p e ra t u res.
*Note gradient feature not available all regions
Kyratec SuperCycler User Manual v3.0
15
7. Configuration Tools
T h e co n f i g u ra t i o n p a ge p r o v i des access t o t h e f o l l o w i n g fea t u res.
FI LE M A N A G E R – F o r m a n a g i n g t h e P C R r u n f i les o n t h e d e v ice a n d c o p y i n g f r o m t h e d e v ice usi n g a U S B
m e m o r y stic k.
M A C H I N E SE T TI N G S – A l l o w s c ha n ges t o b e m a d e t o t h e i ns t r u m e n ts set t i n gs.
TI M E & D AT E – U se d t o set t h e T i m e f o r t h e i ns t r u m e n ts i n te r na l c l o c k.
T H E R M A L C ALI BR ATI O N – T h e t h e r m a l cali b ra t i o n fea t u re is use d t o a d j ust t h e m a c h i n e cali b ra t i o n.
S u pe r C y c le rs are fac t o r y cali b ra te d a n d sh o u l d n o t re q u i re user recali b ra t i o n f o r t h e l i fe o f t h e m a c h i n e. T h e
recali b ra t i o n p r o ce d u re is n o t co vere d i n t h is use r m a n u a l. I f i t is re q u i re d, co n tac t t h e d is t r i b u t o r t h e e q u i p m e n t w as
p u r c h ase d t h r o u g h f o r 'S C Se r v ice n o te – T h e r m a l recali b ra t i o n '. T h e t h e r m a l cal i b ra t i o n is p ass w o r d p r o tec te d t o
p re ve n t i t acci de n ta l l y b e i n g c ha n ge d.
USER AC C OU N TS – U se r acco u n t co n t r o l.
TI M E & D AT E – A l l o w s c ha n ges t o be m a d e t o t h e i ns t r u m e n ts set t i n gs.
SO FT WAR E UP D AT E – A l l o w s u p g ra d i n g o f t h e S u pe r C y c le r o pe ra t i n g S o f t w a re.
TO U C H PA N N E L C ALI BR ATI O N – Y o u ca n also p e r f o r m a cal i b ra t i o n o f t h e t o u c h-scree n i n te r face usi n g t h e
cali b ra t i o n w i za r d. I f t h e cal i b ra t i o n h as va r ie d cl ic k o n t h is b u t t o n a n d f o l l o w t h e i ns t r u c t i o ns t o re-cali b ra te t h e
scree n. I f y o u are h a v i n g d i f f ic u l t ies n a v i ga t i n g t o t h is o p t i o n b eca use t h e t o u c hscree n is o u t o f cal i b ra t i o n t h e n y o u
Kyratec SuperCycler User Manual v3.0
16
m a y use a U S B m o use p l u g ge d i n t o t h e f r o n t U S B p o r t t o reac h t h e t o u c hscree n cali b ra t i o n w i za r d.
7.1 User Accounts
I n t h e users area y o u are a b le t o select a n exist i n g user o r crea te o r d e le te a use r p r o f i le. W h e n a use r is selec te d
t h e r m a l p r o f i les w i l l be l oa de d o r save d t o a d i rec t o r y speci f ic t o t h a t user. L o g g i n g o u t w i l l c ha n ge bac k t o d e fa u l t
user. T o crea te a n e w use r select t h e ' A d d N e w U se r' ic o n a n d t h e n e n te r t h e use r n a m e a n d c h o ose a user p r o f i le
i m a ge.
7.2 File Manager
T h e f i le m a n a ge r area e na b les y o u t o t r a ns fe r P C R r u n f i les b e t w ee n t h e d e fa u l t o r i n d i v i d u a l user d i rec t o r ies a n d a
Kyratec SuperCycler User Manual v3.0
17
U S B m e m o r y d r i ve. P l u g y o u r U S B d r i ve i n t o t h e co n n ec t o r o n t h e f r o n t o f t h e u n i t a n d y o u m a y b r o w se t h e
d i rec t o r ies o n t h e S C a n d t r a ns fe r f i les t o o r f r o m. Y o u m a y also d e le te a n y t h e r m a l p r o f i les f r o m t h e S C. P ress o n
t h e ' H o m e ' ic o ns t o re f res h t h e d i rec t o r y l is t bac k t o t h e r o o t d i rec t o r ies. Se lec t f i les i n ei t h e r t h e S C o r t h e U S B
stic k a n d p ress t h e t r a ns fe r l o g os t o c o p y t h e f i les acr oss. F i les m a y be m o ve d be t w ee n d i f fe re n t user d i rec t o r ies b y
f i rs t co p y i n g t h e m t o a U S B stic k a n d t h e n bac k i n t o t h e d est i n a t i o n user d i rec t o ry.
7.3 Machine Settings
T h e f o l l o w i n g fea t u res are ava ila b le u n d e r t h is p a ge.
96 w e l l t h e r m a l b l o c k set t i n gs :

' Hold at last temperature on run completion' w i l l m a i n ta i n t h e last p r o f i le te m p e ra t u re a f te r t h e r u n h as
co m p le te d. W h e n t h is is n o t selec te d (defa u l t) t h e p e l t ie rs w i l l d eac t iva te a t t h e e n d o f t h e r u n a n d t h e
te m p e ra t u re o f t h e sa m p les w i l l co m e t o rest at a p p r o x i m a te ly t h e i n te r na l h ea tsi n k te m p e ra t u re. T y p i cal l y
t h is w i l l b e a b o u t 35 de g C a t t h e co m p le t i o n o f a r u n. I t is p re fe r re d t o leave t h is o p t i o n u n selecte d as i t
w i l l p r o v i de f o r a l o n ge r p e l t ie r se r v ice l i fe.

'Audible alarm on run completion' t o g g les w h e t h e r t h e re is a n a u d i b le ala r m o r j us t a scree n m essage t o ale r t
t h e o pe ra t o r t h a t t h e r u n h as co m p le te d.

'Use P re d ic te d Temperature for Hold times' T h e S C so f t w a re i m p le m e n ts a t h e r m a l m o d e l w h i c h p re d ic ts t h e
act ual l i q u i d te m p e ra t u re i nsi de t h e reac t i o n vessels. H o l d t i m es ca n b e b ase d o n t h is m o d e l (defa u l t) o r
b ase d p u re l y o n t h e b l o c k te m p e ra t u re. U s i n g t h e p re d ic te d te m p e ra t u re ef fec t ive l y i n c reases t h e h o l d t i m es
(an d t o ta l r u n t i m e) sli g h t l y b u t g i ves a m o r e accu ra te re p rese n ta t i o n o f t h e h o l d t i m e o f t h e act ual sa m p le
l i q u i d. M o s t m a n u fac t u re rs t h e r m a l cycle rs p u re l y use t h e b l o c k te m p e ra t u res f o r t i m i n g.

'Auto Resume Run After Power Outage' I f t h is fea t u re is ava ila b le o n y o u r cycle r (no t availa b le o n all m o d e ls)
t h e n w h e n t h is b o x is c hec ke d a r u n w i l l au t o m a t ical ly resu m e a t t h e sa me p lace i f p o w e r sh o u l d be
m o m e n ta r i l y l os t d u r i n g a r u n.
Kyratec SuperCycler User Manual v3.0
18
T o p h ea te r eva p o ra t i o n co n t r o l set t i n gs :

'Use Top Heater' w i l l ca use t h e t o p h ea te r t o b e use d d u r i n g a r u n i f i t is c he c ke d.

'Wait for top heater' w i l l ca use t h e syste m t o w a i t f o r t h e t o p h ea te r t o ac h ie ve ta r ge t te m p e ra t u re b e f o re
co m m e n c i n g a r u n. W h i l e t h is is h a p pe n i n g a scree n d is p la y i n g t h e t o p h ea te r te m p e ra t u re is d is p laye d a n d
t h e user h as t h e o p t i o n o f sk i p p i n g t h is step.

'Leave heater enabled on run completion' w i l l ca use t h e t o p h ea te r t o b e le f t o n i n d e f i n i te ly a t t h e e n d o f a r u n.
T h is is use f u l i f y o u are h o l d i n g t h e sa m p les at a h i g h te m p e ra t u re o n r u n co m p le t i o n.

E n t r y b o x f o r t o p h ea te r te m p e ra t u re. T e m p e ra t u re is p r o g ra m m a b le be t w ee n 30 – 1 1 0 d e g C (defa u l t 1 0 5
d e g)

B u t t o n f o r m a n u a l l y e na b l i n g t h e t o p h ea te r t o p re h ea t i t i f re q u i re d.

' Disable top heater when set temperature < 30deg' fea t u re is i m p le m e n te d t o re d u ce t h e h i g h t h e r m a l l oa d
p lace d o n t h e p e l t ie rs w h e n h o l d i n g t h e sa m p les a t l o w te m p e ra t u res (ie 4 de g rees). T h is p r eve n ts excess
h ea t f r o m t h e t o p h ea te r h ea t i n g t h e c h i l le d sa m p les. I t is b est t o leave t h is o p t i o n e na b le d.
N o t e t h a t t h e t o p h ea te r is o n l y ac t iva te d w h e n a r u n is sta r te d o r t h e o pe ra t o r e na b les i t i n m a n u a l m o d e.

T h e 'Region Configuration' b u t t o n e na b les t h e O p e ra t i o n a l re g i o n t o b e c ha n ge d sh o u l d t h e i ns t r u m e n t b e
m o ve d t o a d i f fe re n t c o u n t ry. N o t e t h a t availa b le fea t u res va r y be t w ee n d i f fe re n t re g i o ns. See Sec t i o n 2. 1
f o r m o r e i n f o r m a t i o n.
7.4 Software Update
A faci l i t y h as bee n p r o v i de d t o u p d a te t h e so f t w a re w h i c h resi des o n t h e S u pe r C y c ler. T h e u p g ra de f i le y o u rece ive
f r o m o u r su p p o r t tea m o r t h e w e bsi te sh o u l d be p lace d o n t h e m a i n r o o t d i rec t o r y o f y o u r U S B m e m o r y stic k. Y o u
Kyratec SuperCycler User Manual v3.0
19
m a y t h e n select i t t o u p g ra de t h e u n i t’s so f t w a re. A f t e r selec t i n g t h e so f t w a re f i le rea d t h e m essage b o x a n d p ress
O K a n d w a i t. U p g ra de w i l l ta ke u p t o 1 – 2 m i n u tes. O n ce u p g ra de is co m p le te y o u ca n c h ec k t h a t t h e n e w ve rsi o n
is i ns ta lle d b y v isi t i n g t h e 'Information' p a ge f r o m t h e h o m e scree n a n d c hec k t h e i ns ta lle d so f t w a re ve rsi o n.
I M P O R T A N T N O T E : D o n o t i n te r r u p t t h e u n i t w h i le a f i le u p g ra de is i n p r o g ress. S w i t c h i n g o f f t h e p o w e r
d u r i n g u p g ra de m a y re n de r t h e u n i t i n o pe ra b le.
A f t e r u p g ra d i n g so f t w a re t h e i n te r na l o n scree n use r m a n u a l m a y n ee d t o also b e u p d a te d. C l i c k o n o n e o f t h e h e l p
ic o ns, i t w i l l d is p lay a n u p g ra de m essage i f a use r m a n u a l u p d a te is also re q u i re d. T o u p d a te i t y o u n ee d t o d o w n l oa d
t h e co r rec t user m a n u a l z i p f i le f r o m o u r w e bsi te a n d u n z i p i t o n t o t h e r o o t (mai n) d i rec t o r y o n a us b m e m o r y stic k
a n d t h e n i nse r t i t i n t o t h e S u pe r C y c le r a n d f o l l o w t h e u p g ra de p r o m p ts w h e n y o u t r y t o access t h e o u t o f d a te h e l p
syste m.
7.5 Games & Extras
U n d e r t h e ex t ras ta b y o u w i l l f i n d so m e o t h e r p r o g ra ms. T h e Oligo Calculator is use f u l f o r calc u la t i n g t h e
t h e o re t ica l m e l t te m p e ra t u re a n d o t h e r i n f o r m a t i o n p e r ta i n i n g t o t h e o l i g o d esig n. S i m p l y e n te r t h e seq ue n ce a n d i t
d o es t h e rest. Se ve ral p o p u la r Games are also p r o v i de d f o r y o u r e n j o y m e n t!
8 User Tips
I t is reco m m e n d e d b e f o re y o u use t h e d e v ice f o r t h e f i rs t t i m e w i t h b i o l o g ica l sa m p les t h a t y o u ex pe r i m e n t w i t h
set t i n g u p r u ns a n d t r ia ll i n g t h ese t o fa m i l ia r ise y o u rsel f w i t h t h e m a c h i n e t o e nsu re co r rec t o pe ra t i o n o n y o u r
val ua b le sa m p les.
T o ac h ie ve t h e m a x i m u m l i fespa n a n d best cla r i t y f r o m y o u S u p e r C y c le r t o u c h-scree n p lease o n l y use t h e sty l us
p r o v i de d (or eq u i vale n t) o r y o u r f i n ge rs f o r select i n g o n t h e t o u c h-scree n. U s i n g a ba ll p o i n t p e n, p e n c i l o r a n y t h i n g
sha r p w i l l ca use t h e scree n t o scra tc h.
T o m a x i m ise t h e l i fespa n f r o m t h e p e l t ie r d e v ices w h i c h ef fec t t h e h ea t i n g a n d c o o l i n g t h e re are a n u m b e r o f act i o ns
t h e user m a y ta ke:
D o n o t r u n t h e u n i t w i t h n o t u b es i n t h e p la te a n d t h e l i d c l ose d. W h e n t h e l i d is cl ose d a n d n o t u bes are p r ese n t
t h e t o p h ea te r rests cl ose t o t h e 96 w e l l b l o c k a n d w i l l p lace h i g h t h e r m a l l oa d o n t h e p e l t ie r d e v ices. E x t e n d e d
o pe ra t i o n i n t h is sit ua t i o n w i l l sh o r te n t h e l i fespa n o f t h e p e l t ie r d e v ices.
A t t e m p t t o m i n i m ise t h e t i m e h o l d i n g t h e sa m p les a t b e l o w a m b ie n t te m p e ra t u res l o n ge r t h a n re q u i re d. I t is
reco m m e n d e d aga i ns t usi n g t h e d e v ice as a re f r i ge ra t o r t o h o l d sa m p les at 4 d e g rees C te m p e ra t u re f o r l o n g p e r i o ds
Kyratec SuperCycler User Manual v3.0
20
o f t i m e i f y o u w is h t o m a x i m ise t h e l i fespa n o f y o u r i ns t r u m e n t.
T h e h ea te d l i d a p p l ies su bsta n t ial p ressu re t o t h e t u bes t o e nsu re t h e y are w e l l seate d i n t o t h e t h e r m a l b l o c k a n d
p re ve n t t h e ca ps o r seali n g f i l m f r o m p o p p i n g o f f w i t h t h e i n c rease d va p o u r p ressu re i nsi de t h e t u be. W h e n usi n g
o n l y a si n g le o r s ma ll n u m b e r o f i n d i v i d u a l t u b es t h e n i t is a d v ise d y o u p lace a n e m p t y t u b e i n eac h o f t h e 4 co r n e rs
o f t h e b l o c k t o h e l p abso r b t h e p ressu re. T h is w i l l h e l p p re ve n t b u d ge t, o r t h i n w a l l p last ic w a re f r o m d e f o r m i n g
u n d e r t h e h i g h te m p e ra t u re a n d p ressu res.
T h e T o p H e a te r eva p o ra t i o n co n t r o l d e fa u l t is 1 0 5 d e g C . T h is is su i ta b le f o r m o s t a p p l ica t i o ns. I f y o u f i n d t h a t y o u
are ge t t i n g excess co n d e nsa t i o n f o r m i n g a t t h e t o p o f y o u r t u bes y o u ca n t r y i n c reasi n g t h is te m p e ra t u re t o 1 1 0 d e g
C o r h i g h er.
Kyratec SuperCycler User Manual v3.0
21
9 Specifications
T h er mal C ycling Tech nology
H i g h p e r f o r m a n ce act ive h ea t i n g a n d co o l i n g usi n g q u a l i t y
ele m e n ts x 8 & p recisio n se nso rs x 4
T e m p e ra t u re R a n g e
4° C – 9 9° C
T e m p e ra t u re A c c u racy
±0.25° C o f set te m p e ra t u re, 1 m i n u te a f te r ta r ge t
T e m p e ra t u re U n i f o r m i t y
±0.5° C, 30 seco n ds a f te r ta r ge t
T e m p e ra t u re R eso l u t i o n
0. 1° C i n c re m e n ts
H e a t i n g/ C o o l i n g R a te
3.0° C/sec m a x i m u m (bloc k)
W e ll C o n fig uratio n
96-W e l l b l o c k su p p o r t i n g: 0.2 m L t u b es o r st r i p t u bes w i t h f la t o r
d o m e d ca ps; 96-w e l l h i g h- o r l o ws k i r t p la tes w i t h st r i p ca ps, a d hesive
co ver, o r o i l o ve r lay
L i n ea r t h e r m a l g ra d ie n t
P r o g ra m m a b le 0 – 20° C acr oss b l o c k w i d t h (12 w e l ls)
(Availa b le selec te d re g i o ns o n l y)
C o n d e nsa t i o n co n t r o l
A u t o m a t ic u t i l isi n g a p p l ie d p ressu re h ea te d l i d
H e a te d L i d T e m p e ra t u re R a n ge
C o n t r o l la b le 60° C – 1 1 5° C
D i m e ns i o ns
W 180 m m
D 270 m m (10.6”); 340 m m (13.4”) i n c l u d i n g ca b les
H 1 9 0 m m l i d cl ose d (320 m m (12.6”) l i d o pe n)
W eight
5.5 k g (12. 1 l bs)
C olour
p ea r l w h i te o n f r os te d b lac k
E l e c t r i cal
1 0 0 – 240 V A C @ 4 A m p (50/60 H z) A u t o m a t i c v o l ta ge se nse
S ta n d a r d I E C I n le t p l u g
E x te r n a l I n te r face
U S B i n te r face t o W i n d o w s X P / V i s ta base d P C
U S B h os t p o r t f o r m e m o r y stic k, m o u se, k e y b oa r d etc.
I n t e r n a l I n t e r face
E m b e d d e d g ra p h i ca l co n t r o l le r w i t h 7” w i d escree n t o u c h se nsi t ive
co l o u r bac k l i t d is p la y
S o f t w a re
S u p p l ie d w i t h u n l i m i te d use r l ice nse. F ree u p g ra des availa b le v ia w e b
d o w n l oa d.
F u nctio nality
T o u c h d o w n, l o n g-ra n ge, l i nea r t h e r m a l g ra d ie n t, p r o g ra m p a uses,
Kyratec SuperCycler User Manual v3.0
22
te m p e ra t u re g ra p h i n g, te m p e ra t u re l o g g i n g (P C o n l y), P r o f i le l oa d &
savi n g. M a n u a l m o d e
I n c l u d e d A c cesso r ies
P o w e r C a b le, U se r m a n u a l, S t y l us
W a r ra n ty
c hec k w i t h y o u r l o cal d is t r i b u t o r
Kyratec SuperCycler User Manual v3.0
23
10 Terms of Use
E N D USER LICE N C E A GREE M E N T
K y r a tec S u p e r C y c le r S C 2 00
I M P O R T A N T : T H I S E N D U S E R L I C E N C E A G R E E M E N T (or " E U L A ") I S A L E G A L A G R E E M E N T
B E T W E E N T H E P E R S O N , C O M P A N Y, O R O R G A N I Z A T I O N T H A T H A S P U R C H A S E D T H I S
E Q U I P M E N T ("Y O U " O R " C U S T O M E R ") A N D K Y R A T E C . B Y U S I N G T H I S D E V I C E, C U S T O M E R
A G R E E S T O T H E T E R M S O F T H I S A G R E E M E N T. I F Y O U D O N O T A G R E E T O B E B O U N D B Y
T H E S E T E R M S, O R D O N O T H A V E A U T H O R I T Y T O B I N D C U S T O M E R T O T H E S E T E R M S,
T H E N D O N O T C O N T I N U E, A N D R E T U R N T H E D E V I C E T O Y O U R P L A C E O F P U R C H A S E F O R
A R E F U N D I N A C C O R D A N C E W I T H I T S R E F U N D P O L I C I E S.
1. D e f i n i t i o ns
(a) “Su p p l ie r” m e a ns K y r a tec o r o n e o f i ts d is t r i b u t o rs.
(b) “ D e v ice” m e a ns K y r a tec S u p e r C y c le r S C 2 00 T h e r m a l C y c ler.
(c) “ Re g i o n” m e a ns t h e co u n t ry, g r o u p o f c o u n t r ies o r area i n w h i c h d e v ice is l o ca te d.
(d) “Fea t u res” m e a ns so f t w a re o r h a r d w a re o p e ra t i o na l f u n c t i o n a l i ty.
2. L i ce nse G r a n ts
C u s t o m e r is o n l y g ra n te d r i g h ts t o use t h e d e v ice fea t u res w h i c h are o f fe re d t o t h e m i n t h e re g i o n i n w h i c h d e v ice
w as p u r c hase d a n d resi des. C u s t o m e r w a r ra n ts t h a t t h e selecte d re g i o n is t h e t r u e re g i o n o f i ts p u r c hase a n d p h ysical
o pe ra t i o n a l l o ca t i o n. I n c o r rec t select i o n o f re g i o n is i n b reac h o f su p p l ie r te r ms o f l ice nse a n d m a y o r m a y n o t
b reac h a n y 3 r d p a r t y l ice nse te r m s. C u s t o m e r acce p ts sole resp o nsi b i l i t y f o r i n c o r rec t re g i o n select i o n a n d
co nse q ue n ces o f a n y b reac h o f 3 r d p a r t y i n te l lec t ua l p r o p e r t y associa te d w i t h sai d fea t u res.
3. L i m i t a t i o n o f L i a b i l i t y
U se o f d e v ice a n d a n y resu l ts o r i n f o r m a t i o n ge ne ra te d b y d e v ice are at t h e sole r is k o f cust o m er. S u p p l ie r m a k es n o
g ua ra n tees t h a t ex pec te d f u n c t i o ns are p e r f o r m e d c o r rec t ly. S u p p l ie r str o n g l y rec o m m e n ds t h a t c us t o m e r p e r f o r m
f u l l m a n n e r o f i n te r n a l co n t r o ls w i t h i n t h e i r reac t i o n v o l u m es t o satisf y t h e mse lves t h a t ex pec te d f u n c t i o ns w e re
p e r f o r m e d co r rec t ly. S u p p l ie rs sole resp o nsi b i l i t y is f o r re pa i r o r re p lace m e n t o f d e fec t i ve d e v ice w i t h i n t h e w a r ra n t y
p e r i o d. M a x i m u m l ia b i l i t y o f su p p l ie r i n a n y ci rc u ms ta n ce is l i m i te d t o t h e a m o u n t p a i d b y c us t o m e r t o t h e su p p l ie r
f o r t h e d e v ice.
Kyratec SuperCycler User Manual v3.0
24
Was this manual useful for you? yes no
Thank you for your participation!

* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project

Download PDF

advertisement