QIAGEN RNAprotect Cell Reagent, Mini RNeasy Protect Cell Mini Kit Handbook

The RNeasy Protect Cell procedure provides a complete solution for the stabilization and purification of total RNA from sorted or cultured cells. RNA in cells is immediately stabilized using RNAprotect Cell Reagent, and then rapidly purified using the RNeasy Plus Mini Kit, which includes gDNA Eliminator columns for rapid, effective removal of genomic DNA contamination. The stabilized cells can be shipped at ambient temperature prior to RNA purification; no dry ice is required.

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QIAGEN RNAprotect Cell Reagent Handbook | Manualzz

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Key features

  • Stabilizes RNA in cells
  • Removes genomic DNA contamination
  • Stabilizes DNA in cells
  • Provides simultaneous stabilization of RNA and DNA
  • Enables purification of high-quality RNA
  • Suitable for automated purification

Frequently asked questions

Add 5 volumes of RNAprotect Cell Reagent to 1 volume of cell-culture medium or storage solution. Mix by shaking, pipetting, or vortexing. Alternatively, add 300 µl RNAprotect Cell Reagent to the cell pellet in a centrifuge tube and resuspend the cells completely by vortexing.

You can store the mix of cells and RNAprotect Cell Reagent for up to 1 day at 30°C, up to 7 days at room temperature (15–25°C), or up to 4 weeks at 2–8°C, or archive at –20°C or –80°C.

Centrifuge the mix of cells and RNAprotect Cell Reagent for 5 min at 5000 x g in an appropriately sized centrifuge tube. Remove the supernatant completely by pipetting. Loosen the pellet by flicking the tube. Add 350 µl or 600 µl Buffer RLT Plus (see Table 2). Dissolve the pellet completely by vortexing and proceed immediately to step 5.
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