TProfessional TRIO user manual

TProfessional TRIO user manual
Instruction Manual
Ver. 11/12
Model
Ord. No.
TProfessional TRIO 30
TProfessional TRIO 48
TProfessional TRIO combi
070-720
070-723
070-724
Please read these instructions carefully before using this
apparatus!
Biometra GmbH
Rudolf-Wissell-Str. 30
D-37079 Göttingen
Tel.: +49 551-50686-0
Fax: +49 551-50686-66
email: [email protected]
Internet: http://www.biometra.com
Service Department
Rudolf-Wissell-Strasse 14-16
D-37079 Göttingen
Tel.: +49 551-50881-10/12
Fax: +49 551-50881-11
email: [email protected]
This document describes the state at the time of publishing. It needs not necessarily agree with
future versions.
Subject to change!
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Instruction Manual TProfessional TRIO 12/2011
Contents
1
Introduction......................................................................................................................... 6
1.1
Field of Applications ..................................................................................................... 6
1.2
Special features ............................................................................................................ 6
1.2.1
Three independent Thermocyclers in one instrument .......................................... 6
1.2.2
Easy programming................................................................................................ 6
1.2.3
High speed............................................................................................................ 6
1.2.4
Smart lid technology ............................................................................................. 7
1.2.5
USB port ............................................................................................................... 7
1.3
Technical specifications TProfessional TRIO Thermocycler ........................................ 8
1.4
Legal Notes .................................................................................................................. 9
1.4.1
PCR License – Legal Disclaimer .......................................................................... 9
1.4.2
Copyright .............................................................................................................. 9
1.4.3
Liability.................................................................................................................. 9
1.4.4
Meaning of the instructions................................................................................... 9
2
Safety and Warning Notices ............................................................................................ 10
2.1
Definition of Symbols .................................................................................................. 10
2.2
General Safety Instructions ........................................................................................ 10
3
Installation......................................................................................................................... 13
3.1
Content of delivery...................................................................................................... 13
3.2
Unpack and check ...................................................................................................... 13
3.3
Installation conditions ................................................................................................. 13
3.4
Operation Voltage....................................................................................................... 14
3.5
Initial self test.............................................................................................................. 14
4
Operating elements .......................................................................................................... 16
4.1
The TProfessional TRIO Thermocycler front view...................................................... 16
4.2
The TProfessional TRIO Thermocycler rear view....................................................... 17
4.3
The TProfessional TRIO control panel ....................................................................... 17
4.4
The High Performance Smart Lid (HPSL) .................................................................. 18
5
Operating........................................................................................................................... 19
5.1
The TProfessional TRIO user interface ...................................................................... 19
5.1.1
TProfessional TRIO menu buttons ..................................................................... 19
5.1.2
TProfessional TRIO softkeys .............................................................................. 19
5.1.3
Log in menu ........................................................................................................ 20
5.1.4
Block menu ......................................................................................................... 20
5.1.5
System menu...................................................................................................... 21
5.1.6
Program menu .................................................................................................... 21
5.2
TProfessional TRIO software...................................................................................... 22
5.2.1
Log in .................................................................................................................. 22
5.2.2
Create new user account.................................................................................... 24
5.2.3
Supervisor Log in................................................................................................ 24
5.2.4
Delete User......................................................................................................... 25
5.3
Create program .......................................................................................................... 25
5.3.1
Create program from template............................................................................ 25
Instruction Manual TProfessional TRIO 12/2011
3
5.3.2
Create program without template ........................................................................26
5.3.3
Set lid pre-heating mode .....................................................................................26
5.3.4
Set lid temperature ..............................................................................................27
5.3.5
Enter step temperature and time .........................................................................27
5.3.6
Set loop ...............................................................................................................28
5.3.7
Cool below ambient temperature ........................................................................29
5.3.8
Program Pause ...................................................................................................29
5.3.9
Save program ......................................................................................................29
5.3.10
Set temperature optimisation step.......................................................................32
5.4
Edit programs ..............................................................................................................34
5.4.1
Insert program step .............................................................................................34
5.4.2
Delete program step ............................................................................................36
5.4.3
Copy program......................................................................................................37
5.4.4
Delete program....................................................................................................38
5.4.5
Editing programs in the graphical mode ..............................................................40
5.5
Change program options.............................................................................................40
5.5.1
Heating and cooling rate .....................................................................................41
5.5.2
Time increment Æ Δt(s).......................................................................................42
5.5.3
Temperature increment or decrement Æ ΔT(°C) ................................................42
5.6
Run program ...............................................................................................................43
5.6.1
Select program for start from user directories .....................................................43
5.6.2
Quick start programs from the block menu..........................................................45
5.6.3
View program prior to start ..................................................................................46
5.6.4
Display during operation......................................................................................47
5.6.5
Pause Program during run ..................................................................................49
5.6.6
Continue program................................................................................................50
5.6.7
Skip program step ...............................................................................................50
5.7
Stop program...............................................................................................................52
5.7.1
Stop program from a pause.................................................................................52
5.8
USB functions..............................................................................................................53
5.8.1
Connect USB drive to Thermocycler ...................................................................53
5.8.2
Access USB drive................................................................................................53
5.8.3
Delete program in folder USB drive.....................................................................55
5.8.4
Copy program from USB drive ............................................................................55
5.8.5
Copy program to USB drive.................................................................................56
5.8.6
Maximum number of programs on the USB drive ...............................................57
6
System settings.................................................................................................................59
6.1
User configuration .......................................................................................................59
6.1.1
Set language .......................................................................................................59
6.1.2
Change individual PIN .........................................................................................60
6.1.3
Delete account ....................................................................................................60
6.2
System configuration...................................................................................................61
6.2.1
Configure beep....................................................................................................61
6.2.2
Configure display contrast ...................................................................................61
6.2.3
Set time and date ................................................................................................62
6.2.4
Defragment memory............................................................................................62
6.2.5
Screen saver .......................................................................................................63
6.3
System info..................................................................................................................63
6.3.1
System info..........................................................................................................64
6.3.2
View run log files of the last nine runs .................................................................64
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Instruction Manual TProfessional TRIO 12/2011
6.4
Service........................................................................................................................ 66
6.4.1
View log files of Power on self test ..................................................................... 66
6.4.2
Execute block extended self test ........................................................................ 66
6.4.3
View log files from extended self test ................................................................. 67
6.4.4
View Error log files.............................................................................................. 69
6.4.5
Create service info file for the Biometra Service Department ............................. 69
6.5
How to contact Biometra ............................................................................................ 69
6.6
Supervisor system configuration................................................................................. 70
6.6.1
Change user PIN ................................................................................................ 70
6.6.2
Delete user account............................................................................................ 71
6.6.3
Change supervisor PIN....................................................................................... 71
6.6.4
Set boot language............................................................................................... 72
6.6.5
Delete all user accounts ..................................................................................... 72
7
Short manual..................................................................................................................... 74
8
Trouble shooting .............................................................................................................. 79
8.1
Slow heating and cooling............................................................................................ 79
8.2
Autorestart .................................................................................................................. 79
8.3
Restart due to unrecognised power failure ................................................................. 79
8.4
Adaptation of protocols from other cyclers ................................................................. 79
8.5
Releasing wheel in case of blocked lid ....................................................................... 79
9
Maintenance and repair ................................................................................................... 81
9.1
Cleaning and Maintenance ......................................................................................... 81
9.2
Servicing and repair.................................................................................................... 81
9.3
Firmware update......................................................................................................... 81
9.4
Replacement of spare parts ....................................................................................... 81
10 Accessories ...................................................................................................................... 82
10.1 Plasticware ................................................................................................................. 82
11 Service............................................................................................................................... 83
11.1
Instructions for return shipment .................................................................................. 83
11.2 Packing of the Thermocycler ...................................................................................... 84
12 Equipment Decontamination Certificate ........................................................................ 85
13 Note for the disposal of electric / electronic waste....................................................... 87
14 EU – Konformitätserklärung / EU - Declaration of Conformity .................................... 88
15 Warranty ............................................................................................................................ 89
16 Subject Index .................................................................................................................... 90
Instruction Manual TProfessional TRIO 12/2011
5
1 Introduction
1.1 Field of Applications
The Biometra TProfessional TRIO is a Thermocycler licensed for PCR applications (research
use only) and intended to be used for performing polymerase chain reactions. The instrument
continues the Biometra line of triple block Thermocyclers and combines the intuitive user
software of the TProfessional family with a completely new hardware structure that delivers
highest performance. Due to an improved air stream design the exhaust is now located at the
rear and higher temperature uniformity and heating and cooling rates are achieved. For easy
administration the TProfessional TRIO offers versatile USB functions. The result is a high-end,
easy to use Thermocycler with excellent technical specifications.
1.2
1.2.1
Special features
Three independent Thermocyclers in one instrument
The TProfessional TRIO Thermocycler offers three independent blocks in one housing thus
different independent protocols can be run at the same time. Moreover by the new temperature
optimisation step (TOS), that makes use of the multiblock function of the instrument, program
steps, delivering three different annealing temperatures, can be created easily. This makes the
TProfessional TRIO Thermocycler the perfect instrument for laboratories with the demand for
high flexibility and the need to often optimise new PCR-protocols. With a maximum capacity of 3
x 48 wells the TProfessional TRIO also offers high throughput in parallel operation. The
TProfessional TRIO Thermocycler is available in three different block versions for 0.2 ml tubes,
0.5 ml tubes or both in the combi block.
1.2.2
Easy programming
The TProfessional TRIO Thermocycler offers the intuitive TProfessional software with easy
spreadsheet programming philosophy. New programs are easily created in a well arranged
screen that avoids the need to toggle between different windows. This makes the creation of
new or editing existing programs fast and easy. One touch leads from the spreadsheet to an
alternative graphical programming mode. Four softkeys directly below the display offer quick
access to all functions needed in the individual context and four menu buttons above the display
to the main menus. For easy retrieval, programs can be stored in 30 individual subdirectories
(optionally password protected). By the administrator function the accumulation of unused
programs or user directories can be managed to keep the memory content up-to date.
Keyboard and display are arranged at an angle to ensure reflection-free viewing and ergonomic
programming.
1.2.3
High speed
By the elegant housing with improved air stream design and a completely new hardware
environment the TProfessional TRIO achieves higher heating and cooling rates and better
temperature uniformity. High ramping rates provide both short experimental times and
increased specificity. The instrument works quietly and consumes little power, which in turn
leads to low heat emission and by the compact footprint it saves valuable bench space.
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Instruction Manual TProfessional TRIO 12/2011
1.2.4
Smart lid technology
The TProfessional TRIO Thermocyclers heated lids prevent condensation and provide reliable
contact between samples and thermoblock. The lids are powered by Biometra‘s well known
Smart Lid technology with integrated clutch mechanism which automatically limits the lid
pressure applied to the plasticware and thus saves tubes from damage. In addition by formation
of a homogeneous air cushion an even temperature distribution between the samples is
ensured, significantly improving temperature uniformity. This also ensures reproducible
conditions amongst different PCR runs.
The temperature of the independently working lids can be set individually in a range between 30
and 99°C. This ensures optimal conditions also for other applications as PCR like e.g.
enzymatic restriction reactions. With one press on the front button, the lids gently swing open
and arrest in their end position. In combination with the now easier to access blocks this
provides a maximum of convenience to the customer.
1.2.5
USB port
By using an USB stick protocols can be exchanged between instruments. Therefore by the USB
functions it is very easy to synchronize the memory contents of instruments.
Instruction Manual TProfessional TRIO 12/2011
7
1.3 Technical specifications TProfessional TRIO Thermocycler
Order number
070-720
070-723
070-724
TProfessional TRIO 30
TProfessional TRIO 48
TProfessional TRIO combi
3 blocks for 30 x 0.5ml
tubes each
3 blocks for 48 x 0.2ml
tubes
or 48 well microplates
or 6 x strips of 8 each
3 combi blocks for 18 x 0.5ml
tubes**
or 48 x 0.2ml tubes,
or 48 well microplates
or 6 x strips of 8
Max. Heating rate*
4.0 °C/sec
5.0 °C/sec
3.0 °C/sec
Avg. Heating rate*
3.6 °C/sec
4.2 °C/sec
2.7 °C/sec
*
3.6 °C/sec
4.5 °C/sec
2.7 °C/sec
*
3.2 °C/sec
3.8 °C/sec
2.4 °C/sec
Capacity
Max. Cooling rate
Avg. Cooling rate
Temperature
Uniformity*
± 0.6 °C at 95 °C
± 0.3 °C at 70 °C
± 0.2 °C at 55 °C
Temperature
range
3°C to 99°C
Multiblock tool
Control accuracy
Blocks
Software
Program memory
Display
Auto restart after
power failure
Cool samples at
4°C
Heated lid
Lid temperature
range
Power
consumption
Noise emission
Interfaces
Dimensions
(W x D x H)
Weight
Temperature Optimisation Step (TOS)
0.1°C
Aluminium
Toggle between easy spreadsheet and graphical mode, adjustable ramp rates, time
and temperature increments, extended self test, service info files, USB functions.
30 individual subdirectories, 350 average programs
¼ VGA screen, 320 x 240 pixel
Yes
Yes
High Precision Smart Lid for optimal lid pressure and excellent temperature
uniformity
30°C to 99°C
1000 Watt
Very low
1x USB A Port, 1x USB B Port
30 cm x 41 cm x 25 cm
17.3 kg
Working
5 – 35°C, 70% relative humidity
conditions
*
According to Biometra standard procedure.
**
Capacity increases to 35 x 0.5 ml tubes by use of small cap tubes
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Instruction Manual TProfessional TRIO 12/2011
1.4 Legal Notes
1.4.1
PCR License – Legal Disclaimer
Purchase of a Biometra Thermocycler conveys a limited non-transferable immunity from suit for
the purchaser’s own internal research and development and applied fields other than human in
vitro
diagnostics
under
one
or
more
of
US
Patents
Nos.
5,038,852, 5,656,493, 5,333,675, 5,475,610, and 6,703,236, or corresponding claims in their
non-US counterparts, owned by Applera Corporation.
No right is conveyed expressly, by implication or by estoppel under any patent claim, reagents,
kits, or methods such as 5´ nuclease methods, or under any other apparatus or system claim,
including but not limited to US Patent No. 6,814,934 and its non-US counterparts, which
describe and claim thermal cyclers capable of real-time detection.
Further information on purchasing licenses may be obtained by contacting the Director of
Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Biometra biomedizinische Analytik GmbH
Rudolf Wissell Str. 30
37079 Goettingen, Germany
1.4.2
Copyright
All rights reserved. It is not allowed to copy and publish the manual or parts of it in any form as
copies, micro film or other methods without a written authorisation from Biometra.
Biometra is pointing out that applied company and brand names are usually protected trade
marks.
1.4.3
Liability
Biometra is not liable for damages and injuries caused by use not considering these operating
instructions in parts or completely.
1.4.4
Meaning of the instructions
Biometra recommends that you first read these instructions carefully. This operation instruction
is part of the product and should be kept over the full life-time of the instrument. It should also
be forwarded to subsequent owners and users. Make sure that additions and updates are
inserted into the operation instructions.
Instruction Manual TProfessional TRIO 12/2011
9
2
Safety and Warning Notices
2.1 Definition of Symbols
Symbol
Definition
Caution! Refer to instruction manual!
Danger! High voltage!
Fragile!
Danger! Hot surface!
2.2 General Safety Instructions
Please read this manual carefully before starting operation of the TProfessional TRIO
Thermocycler. The TProfessional TRIO Thermocycler is intended for sample incubation at
varying temperatures.
•
General safety precautions for laboratory work must be observed when working with the
TProfessional TRIO Thermocycler.
•
The TProfessional TRIO does not produce a sound power level that could be hazardous for
the user.
The thermoblock and the heated lid will reach high temperatures
during operation. Both thermoblock and heated lid can burn you.
Rapid heating of the thermoblock can cause liquids to boil
explosively. Always wear safety goggles during operation. Close the
lid before starting a program.
Do not heat samples without having the lid locked securely.
Be aware that samples are reaching high temperatures. Do not touch
or open hot tubes or microplates because hot liquid may quickly spill
out.
Do not touch the heated lid.
Use only suited plastic ware in the TProfessional TRIO
Thermocycler. Tubes and plates must show good fit when placed in
the thermoblock. Only use tubes that are suited for high
temperatures (tight lids).
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Instruction Manual TProfessional TRIO 12/2011
The TProfessional TRIO Thermocycler contains no user serviceable
parts. Do not open the instruments housing. Service and repair may
only be carried out by the Biometra Service department or otherwise
qualified technical personal.
Do not use the instrument when damages of the housing, block,
cable or other parts are visible.
Prior to connecting the unit to the power source please ensure that
the voltage selector at the bottom of the instrument is set to the
required voltage.
Make sure that the main supply voltage is in accordance with the
label above the power connection (see section 4.2)
Unplug the power cable before you open the TProfessional TRIO
Thermocycler. Danger of electric shock!
Make sure that the appliance connector and the plug of the supply
cord are accessible, so you can separate the instrument from the
mains.
Connect the TProfessional TRIO Thermocycler to a grounded
socket.
When only few samples are put in the block place additional tubes in
the four corner positions. This is to evenly distribute the lid pressure
and prevents single tubes from excessive pressure. Use of few tubes
may result in damage of the tubes by excessive pressure.
Appropriate safety regulations must be observed when working with
infectious, pathogenic or radioactive material. Ask the responsible
local safety inspector for details.
The TProfessional TRIO Thermocycler must not be used with
explosive, flammable or volatile liquids.
Do not place fingers between lid and housing when opening or
closing the lid.
Before opening of the lid, release lid pressure (see section 4.4)
It is not necessary to apply oil into the opening of the block in order
to improve the heat transfer between the block and the sample
tubes.
If you still decide to use oil, do not use silicon oil. Mineral oil may be
used.
Ensure that both the rear and bottom ventilation slits not clogged by
dust or other material. Danger of overheating!
Let equilibrate the TProfessional TRIO Thermocycler to room
temperature before starting operation.
There must be sufficient distance between the ventilation slots on the
rear of the Thermocycler and a wall or another instrument (min 10
cm). Danger of overheating!
This instrument is designed and certified to meet EN 61010-1 safety
standards. It should not be modified or altered in any way. Alteration
Instruction Manual TProfessional TRIO 12/2011
11
of this instrument will void the warranty, void the EN61010-1
certification, and create a potential safety hazard.
Place the TProfessional TRIO Thermocycler on a stable, non
flammable surface in a dry, safe environment. For details see
working conditions in table “Technical specifications” (see chapter
1.3).
Do not use alcohol (e.g. methanol, ethanol), organic solvents or
abrasives to clean the instrument.
For transports always use the original Biometra box.
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Instruction Manual TProfessional TRIO 12/2011
3
Installation
3.1 Content of delivery
1)
2)
3)
4)
Thermocycler
Mains connector
Manual
Short Manual
Please keep the original transport box for return shipment in case of servicing. The
TProfessional TRIO shipping box provides a specially developed system for contact-free
transport of this electronic device.
3.2 Unpack and check
Unpack and carefully examine the instrument. Report any damage to Biometra. Do not attempt
to operate this device if physical damage is present.
Please keep the original packing material for return shipment in case of service issues
!! Attention !!
Please fill out and send back the warranty registration card. This is important for you to
claim full warranty.
3.3 Installation conditions
•
Place the TProfessional TRIO Thermocycler on a stable surface in a dry, safe environment.
For details see working conditions in table “Technical specifications” (see chapter 1.3).
•
Let equilibrate the TProfessional TRIO Thermocycler to room temperature before starting
operation (1 to 6h).
•
Make sure that the appliance connector and the plug of the supply cord are accessible, so
you can separate the instrument from the mains.
•
Make sure that the ventilation slots on the bottom and the rear are not obstructed (see
section 4.2). Make sure that there is no object underneath the thermocycler that may block
the ventilation slots at the bottom (e.g. a piece of paper etc.)
•
There must be sufficient distance between the ventilation slots at the rear of the
Thermocycler and a wall or another instrument (min 10 cm).
Ensure that both the side and bottom ventilation slits of the
rear and bottom of the instrument are unobstructed.
Insufficient ventilation can cause overheating of the
instrument.
Instruction Manual TProfessional TRIO 12/2011
13
•
Make sure that the main supply voltage is in accordance with the label above the power
connection (see section 3.4)
•
Connect the TProfessional TRIO Thermocycler to a grounded socket.
Prior to connecting the unit to the power source please ensure that the
voltage selector at the back side of the instrument is set to the required
voltage.
Danger of electric shock! Unplug the power cable before you open the
TProfessional TRIO Thermocycler.
•
The display contrast can be adjusted to local light conditions (6.2.2).
3.4 Operation Voltage
Important: Prior to connecting the TProfessional TRIO to the mains, make sure that the setting
of the Voltage selector is in accordance with your mains Voltage.
The TProfessional TRIO Thermocycler can operate at 100, 115 or 230 Volt. The operation
Voltage is shown on the Voltage selector which is located at the instrument bottom.
To change operation Voltage of the TProfessional TRIO, switch off the instrument and
disconnect the mains plug.
Use a coin or another round shape item to turn the adjustment slot of the Voltage selector to the
new Voltage.
Instrument bottom
Instrument backside
Voltage indicator
Mains plug
Fuses
110
Fuses
11
5
230
Adjustment slot
Power switch
1
2
3.5 Initial self test
After switching on the TProfessional TRIO the serial number of the instrument and the software
version are displayed.
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Instruction Manual TProfessional TRIO 12/2011
A log file of the power on self test is stored in the Thermocycler memory (see section 6.4.1).
Instruction Manual TProfessional TRIO 12/2011
15
4 Operating elements
4.1 The TProfessional TRIO Thermocycler front view
16
Instruction Manual TProfessional TRIO 12/2011
4.2 The TProfessional TRIO Thermocycler rear view
4.3 The TProfessional TRIO control panel
Instruction Manual TProfessional TRIO 12/2011
17
4.4 The High Performance Smart Lid (HPSL)
To achieve optimum pressure on the tubes the TProfessional TRIO is equipped with a height
adjustable heated lid.
Close the lid:
After the samples have been placed in the block close the lid. Turn the wheel clockwise until
you hear a clicking noise. In this mode the pressure will not increase further, even when you
keep on turning the wheel.
Note: The pressure of the lid has been optimized for a fully loaded block. If only very few tubes
are loaded to the block you should place dummy tubes in the four corner positions to avoid
damage of tubes by excessive pressure.
Open the heated lid:
First: Release pressure by turning the wheel counter clockwise. As soon as there is no more
resistance the pressure has been released.
Then: Now you can open the lid with the knob.
Important: The lid should not be opened under pressure because this leads to damage of the
locking mechanism.
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Instruction Manual TProfessional TRIO 12/2011
5 Operating
5.1 The TProfessional TRIO user interface
The TProfessional TRIO user interface provides four menu buttons above the screen and four
softkeys below the screen
5.1.1
TProfessional TRIO menu buttons
The four menu buttons allow quick access to the TProfessional TRIO main menus. These are:
•
•
•
•
Login /out
System
Block
Program
menu
menu
menu
menu
The active menu is indicated by a graphical link to the respective menu button.
The example shows Program menu is active.
The four menu buttons are permanent and always have the same function. This is in contrast
to the softkeys. The softkey functions change depending on the current software menu.
5.1.2
TProfessional TRIO softkeys
Below the display there are four softkeys.
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19
In contrast to the menu buttons, the softkeys have changing functionalities, which are shown
in the above display line.
5.1.3
Log in menu
To enter the Log In menu, press the menu button [Log in/out] above the display.
When the instrument is switched on, the log in screen is displayed. To use the instrument a user
has to be selected. Alternatively, a new user can be created.
5.1.4
Block menu
To enter the Block menu, press the menu button [Block] above the display.
The Block menu shows the current status of each of the three blocks. Each block can be active
with a program running or free.
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Instruction Manual TProfessional TRIO 12/2011
5.1.5
System menu
To enter the System menu, press the menu button [System] above the display.
The System menu allows global settings and provides information about the instrument. The
power on self test log files can be retrieved and service files generated, a detailed description of
this menu is shown in section 6.
5.1.6
Program menu
To enter the Program menu, press menu button Program above the display.
In the Program menu, existing programs can be edited or new programs can be created. Each
user has its own user directory where individual programs are saved. Programs from other user
directories can be used or copied but not modified.
Instruction Manual TProfessional TRIO 12/2011
21
5.2 TProfessional TRIO software
The TProfessional TRIO Thermocycler features easy spread sheet programming. This means
that all program parameters are entered into a simple central spreadsheet without the need to
toggle between different screens.
Four cursor keys provide easy navigation within the software. The cursor keys LEFT and RIGHT
have additional functions as described below:
The right cursor key moves the cursor to the next field.
This cursor can also be used to complete data entry. By pressing the right
cursor key settings will be taken over and the cursor moves to the next field.
In the file directory, this key moves to cursor forward to the next (lower) level.
The left cursor key moves the cursor back to the previous field.
In most screens this cursor is equivalent to the [Back] softkey on the control
panel (see chapter 4.3).
In the file directory, this key moves the cursor back to the higher level.
5.2.1
Log in
The TProfessional TRIO manages up to 30 individual users. For each user a directory is
created where programs are stored. Programs from other directories (i.e. users) can be read
and copied but not modified. This allows to use all programs that are in the cycler memory, but
to write only in the own user directory.
Each user is identified by his user name (max. 15 digits) and initials (2-3 letters).
Press menu button [Log in/out] above the display. A list of users is shown:
Select user with cursor keys or enter first initial. The cursor jumps to the first account starting
with this letter.
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Instruction Manual TProfessional TRIO 12/2011
Press softkey [Log in] and (if the directory is PIN-code protected) enter the PIN-code.
Confirm PIN with softkey [PIN-code OK]. Then the currently logged-in user is shown:
To proceed with editing or running a program, select the menu button [Program] or [Block]
above the display.
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23
5.2.2
Create new user account
Press menu button [Log in/out] above the display.
To create a new user account press softkey [New User].
Enter your name and 2-3 initials. Enter a personal identification number (PIN) consisting of 1-7
digits. For security reasons, you are requested to repeat the entry of your PIN.
Note: PIN entry is optional. If no password protection is wanted, just confirm with [User-Data
OK]. However, if no PIN is entered, you account containing all of your programs can be
modified by unauthorized personal.
Note: Your PIN can be changed any time in the System menu (see section 6.6.1).
5.2.3
Supervisor Log in
Only the supervisor is authorized to change global instrument settings. This includes update of
firmware and the deletion of user accounts (see section 6.6).
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To log in as supervisor, press the [Log in/out] menu button above the display. Press softkey
[Supervisor Log in], enter supervisor PIN and confirm with [PIN-code OK].
The default supervisor PIN is 000 000 0 (do not enter blanks).
5.2.4
Delete User
Once logged in, you can delete your own account in the System menu (see section 6.1.3).
In addition, any user can be deleted by the supervisor. To delete a user, log in as supervisor
(see section 6.6.2) and select menu option “delete user”. Choose the user account you would
like to delete and confirm with [OK].
Note: By deleting a user account all programs in the referring user directory will be deleted. It is
therefore recommended to safe the programs in a different directory prior to deleting a user
account.
5.3 Create program
At Thermocyclers of the TProfessional series programs can be created in two ways. Preinstalled program examples may be used (see chapter 5.3.1) or new programs without a
template created (see chapter 5.3.2).
5.3.1
Create program from template
Log in to your personal user directory and press menu button [Program] above the display to
enter the TProfessional TRIO program menu. The directory “Biometra Shared” contains several
pre-installed protocols for different PCR applications.
Press [Open Directory] to open the directory „Biometra Shared“. Choose a suitable program by
navigating with the cursor keys or enter the corresponding program number. Protocols may be
started directly from the Biometra Shared directory or may be copied to the respective user
directory and edited (see chapter 5.4.3)
Note: Please always, before starting a template protocol, check the single steps and adapt
details (times, temperatures, loops, temperature- and time-increments) according to the
required PCR conditions respectively. Biometra will not be liable if the PCR does not work if
necessary adjustments have not been programmed.
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Note: The directory is protected by a PIN-Code. The default PIN for the directory “Biometra
Shared” is 0000.
5.3.2
Create program without template
Press menu button [Program] above the display (see chapter 5.1) to enter the TProfessional
TRIO Program menu.
Press softkey [New Program]. The easy programming spreadsheet opens (see chapter 5.3.3).
5.3.3
Set lid pre-heating mode
Before the program starts the lid can be pre-heated. The pre-heating ensures the formation of a
homogeneous tempered air cushion between the samples and avoids evaporation during the
initial heating phase.
Note: During the pre-heating phase of the lid, the block is held constant at 25°C.
Note: After reaching the set lid temperature, there is a 40 second equilibration phase before the
block starts.
Note: The default setting, which is recommended for most applications, is pre-heating ON.
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However, if the program should already start while the lid is heating up, set the pre-heating
mode OFF using softkey [Preheat ON/OFF].
Confirm the settings for the heated lid with [Enter] or step to the next field with the right cursor
key.
5.3.4
Set lid temperature
Note: Thanks to the design of the High Performance Smart Lid, lower lid temperatures can be
used for thermocycling than in the past. Thanks to the lower lid temperature a higher
temperature uniformity between samples is be achieved.
The recommended lid temperature is 99.0°C.
Enter temperature and confirm with [Enter] or step to the next field with the right cursor key.
5.3.5
Enter step temperature and time
Use cursor keys to navigate within the programming table.
Note: Each setting is confirmed with [Enter]. The cursor moves automatically to the next field.
Alternatively, you can confirm a value by moving forward with the cursor keys.
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Enter temperature for the first step and press [Enter]. In the next column you can enter the time
for this temperature:
Note: Minutes and seconds can be separated by “dot”, but this is optional. You can also enter
the digits one after the other.
Example: To set 2 minutes, 30 seconds enter “2”, “3”, “0”. Subsequently 2:30 [m:s] will be
displayed in the programming spreadsheet.
Note: For Biometra Thermocyclers a dot can be used as a shortcut to program minutes.
Example: To set 2 minutes enter “2”, “•”. Subsequently 2:00 [m:s] will be displayed in the
programming table
5.3.6
Set loop
Note: In general, loops are entered in the LAST STEP of the loop. A loop is defined by
selecting the FIRST STEP in the loop plus the number of back loops.
Enter the number of the first step within the loop in the column “goto” and confirm with [Enter].
Enter the number of back loops in the column “loops”.
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The loop is shown as a bracket at the left side of the spreadsheet.
Note: Total cycler number = (number of back loops) + 1, e.g. enter 24 for a total cycle number
of 25.
5.3.7
Cool below ambient temperature
After the program has been finished, samples can be kept at below ambient temperature.
Program a pause to keep the temperature for an undefined time (see chapter 5.3.8).
Note: To save lifetime of Peltier elements, a temperature of 16°C for the final step rather than
4°C is recommended.
5.3.8
Program Pause
To hold a temperature for an indefinite time enter “0”. After confirming with [Enter] the word
“Pause” is shown.
5.3.9
Save program
Press button [Save / Save as].
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The first available program number is automatically pre-selected. Use cursor keys (or enter two
digits) to move to a different program store.
To enter a program name press button [Edit Name].
Enter program name and confirm with button [OK].
Confirm program store number with softkey [Save].
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Program number, name and date are shown in the file directory. To run the program press
menu button [Choose Block].
Choose a block using the softkeys [Block 1], [Block 2] or [Block 3].
The selected block is shown in the display.
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Press the [Start] button on the left side of the control panel (see also chapter 4.3) to start the
program on the selected block.
Optionally programs can also be started on several blocks simultaneously. To select more than
one block press [Multiblock Selection].
Select/deselect blocks with the corresponding softkeys or use the cursor keys left/right. Confirm
with [Confirm selection].
Press the [Start] button on the left side of the control panel (see also chapter 4.3) to start the
program on the selected blocks.
5.3.10 Set temperature optimisation step
For the optimisation of new PCR protocols very often gradient enabled Thermocyclers are used.
The TProfessional TRIO offers no gradient function but by the temperature optimisation step
(TOS) function pograms can be created, making use of the three blocks and delivering different
annealing temperatures at the defined step.
The easiest way to define a temperature optimisation step is to enter two temperatures
separated by “-“. Confirm with [Enter] or move cursor to the next field. The set temperatures
become separated by a step-like icon.
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Alternatively, move cursor to the desired temperature optimisation step and press [Edit
TempOpti].
In the temperature optimisation screen an annealing temperature and an increment can be set.
The annealing temperature defines the temperature at this step for block 2 whereas the
increment defines the temperatures for block 1 and block 3. Confirm the settings with [Enter].
After the settings are confirmed the temperatures for the three blocks at this step are displayed.
To save the program press [Save/Save as]. For easy recognition by the user all temperature
optimisation protocols get the extension “TOpt”.
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Note: To start programs containing a temperature optimisation step all three blocks are
required. Therefore please ensure that none of the blocks is currently in use. The program will
be started simultaneously on all blocks when the [Start] button on the control panel (see also
chapter 4.3) is pressed.
5.4 Edit programs
Programs can be edited in the programming spreadsheet or graphical mode. To toggle between
these two modes use the softkeys [Edit Table] or [Edit Graph] respectively. The graphical
mode is intended for easy modification of existing programs, which can then be saved with a
new program number and name. To edit programs in the graphical mode see section 5.4.5
Note: During operation of the Thermocycler the active program can be viewed but not modified.
If you want to change settings of the active program for further experiments you have to save a
copy of this program. For further information about copying programs see section 5.4.3.
5.4.1
Insert program step
Move cursor to the position, where a new step should be inserted.
To insert a program step, press softkey [Insert/Delete Step]. The step at which a new step will
be inserted appears highlighted.
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Press [Insert Step].
Confirm with softkey [OK]. Enter temperature and time settings for new step.
Note: The new step has been entered within an existing loop. This loop now consists of four
steps.
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5.4.2
Delete program step
Move cursor to the step that should be deleted and press softkey [Insert/Delete Step].
The step to be deleted appears highlighted.
To delete the highlighted step, press softkey [Delete Step].
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Confirm with softkey [OK].
Note: The new step has been deleted within an existing loop. This loop now consists of only 3
steps.
5.4.3
Copy program
It is possible to copy programs from other user directories into the own user directory. Press
menu button [Program] above the display and select a user directory. Press [Open Directory].
Select a program to copy with the cursor keys.
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Press softkey [Copy Program]. The cursor automatically jumps to the next available (free)
program store in the directory of the currently logged-in user.
The copied program can be stored at any storage number. To change storage number, move
the cursor to the wanted location or directly enter a number (two digits) at the keypad.
If desired edit the program name [Edit name].
Confirm to save the program with [Save Copy].
5.4.4
Delete program
Select a program from a user directory.
Note: Programs in pin-code protected user directories cannot be deleted.
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Press softkey [Delete Program].
Confirm deletion of the selected program with [OK].
This program store is now free.
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5.4.5
Editing programs in the graphical mode
Programs are created in a programming spreadsheet where new steps are easily defined by
temperature and time. In addition, programs can be edited in the graphical mode which provides
a schematic temperature plot of the different steps.
To toggle between this two modes use the softkeys [Edit Table] or [Edit Graph].
Spreadsheet mode [Table]
Graphical mode [Graph]
To change temperature and time settings, move cursor to the respective field and enter new
values with the key pad. Once the desired change has been made the temperature graph is
updated.
Note: The steps are automatically spread over the whole range to give maximum resolution
(thus steps height does not represent the real temperature).
Note: Loops can only be set the spreadsheet mode (press [Edit Table])
Note: To insert or delete program steps, go to the spreadsheet mode (press [Edit Table])
Note: To enter a temperature optimisation step, move cursor to the respective step and press
[Edit Temp Opti]. Enter an annealing temperature and an increment as described in section
5.3.10.
5.5 Change program options
For each step additional options can be defined.
To change program options, move the cursor to the right side of the spreadsheet, labelled with
“Options”. An expansion of the table is displayed.
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To go back to programming spreadsheet, move the cursor to the left side of the display
If options have been changed from the default values, there is a “+” as indication in the options
field.
5.5.1
Heating and cooling rate
Since the TProfessional TRIO is a very fast thermocycler it may be necessary to reduce the
heating and cooling ramp to adopt protocols from slower thermocyclers.
The average heating or cooling rates can be set in the column labelled with Ê[°C/s].
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Note: This entry specifies at which step the set speed is achieved. In example to heat from step
2 to step 3 with the desired speed, the heating rate in step 3 has to be lowered.
Note: To reduce the over all speed of the Thermocycler, the heating rate has to be modified in
all steps. The speed settings will be only valid for this individual program.
5.5.2
Time increment Æ Δt(s)
To compensate for loss in enzyme activity, each step within a loop can be extended from cycle
to cycle. Enter the desired time increment (seconds) in the column labelled with dt(s). This value
will be added to the time value from cycle to cycle.
Note: Be sure that the time increment is set in a step that lies within a loop. Otherwise there will
be no iterative time increase.
Note: A time increment will increase the total runtime depending on the numbers of cycles and
the size of the increment. A program with many cycles and large time increments will take a
significantly longer time than a standard protocol.
5.5.3
Temperature increment or decrement Æ ΔT(°C)
For some applications it is useful to start with a higher temperature and to decrease this
temperature from cycle to cycle. This subsequent lowering of a temperature is called touch
down.
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To decrease a temperature from cycle to cycle enter a negative temperature increment in the
column labelled with ΔT(°C).
Note: Be sure that the temperature decrease is set in a step that lies within a loop. Otherwise
there will be no iterative temperature decrease.
5.6 Run program
To start a program press the [Start] button on the control panel (see chapter 4.3).
This button is only active, when a program has been selected for start. This is indicated by the
green LED in the start button.
5.6.1
Select program for start from user directories
Any program can be started by any user. Also programs from other user directories can be
started.
To select a program for start press the [Program] menu button above the display.
Open a user directory [Open Directory] and select the program to start. Press [Choose Block].
To choose a block press the corresponding softkey.
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The selected block is displayed.
Optional: To select several blocks use the function [Multiblock Selection]. Confirm the
selection with [Confirm Selection].
To start the program on the selected blocks press the [Start] button on the control panel (see
chapter 4.3).
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Note: To start programs containing a temperature optimisation step (programs with extension
TOS) all three blocks are required. Therefore please ensure that none of the blocks is currently
in use (see chapter 5.3.10).
5.6.2
Quick start programs from the block menu
The TProfessional TRIO provides the last 5 programs that haven been run for quick start.
Press the [Block] menu button above the display (see chapter 5.1.2). The block selection
screen opens:
To select a block press the corresponding softkey or use the cursor keys left/right. Then select
one of the last five programs with the cursor keys.
Optionally programs can also be started on several blocks simultaneously. To select more than
one block press [Multiblock Selection].
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Select/deselect blocks with the corresponding sofkeys. Confirm with [Confirm selection].
Press the [Start] button on the left side of the control panel (see also chapter 4.3) to start the
program on the selected blocks.
Note: To start programs containing a temperature optimisation step (programs with extension
TOS) all three blocks are required. Therefore please ensure that none of the blocks is currently
in use (see chapter 5.3.10).
5.6.3
View program prior to start
To check the program prior to start press softkey [View Program].
The current program, the estimated run time and the selected blocks are shown.
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To display the temperature optimisation step settings use the cursor keys to select the
corresponding program step and press [View TempOpti].
To toggle between the graphical and spreadsheet mode use the softkeys [View Table] and
[View Graph].
Spreadsheet mode [Table]
Graphical mode [Graph]
Note: In the program preview mode a program cannot be edited. To edit programs press menu
button [Program] above the display (see chapter 5.1.2).
5.6.4
Display during operation
After at least one block has been started, the following screen is shown:
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The status of blocks can be free, active or paused. The user that started the program and the
estimated run time are shown. To display detailed information for a running program press the
corresponding softkey [Block 1], [Block 2] or [Block 3].
Three different display modes are available during the run:
Show Time
Show Graph
Show Table
To toggle between the modes use softkeys [Show Time], [Show Graph] and [Show Table].
To access the block temperature overview screen press [Overview Temperatur.].
The actual block and lid temperature and step are displayed.
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5.6.5
Pause Program during run
To pause a program press the corresponding softkey [Block 1], [Block 2] or [Block 3].
Press [Pause Block/Skip Step].
Press [Pause Block].
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The message “Pause” for the corresponding block is displayed.
5.6.6
Continue program
To continue a program press the corresponding softkey [Block 1], [Block 2] or [Block 3].
To continue the paused program press softkey [Continue].
5.6.7
Skip program step
To skip a program step press the corresponding softkey [Block 1], [Block 2] or [Block 3].
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Press [Pause Block/Skip Step].
Press [Skip Step].
The actually performed step will be skipped. The program continues with the next step.
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5.7 Stop program
To stop an active program press the [Stop] button on the control panel (see chapter 4.3).
To stop a program step press the corresponding softkey [Stop Block 1], [Stop Block 2], [Stop
Block 3] or press softkey [Stop all blocks].
Confirm with [OK].
5.7.1
Stop program from a pause
To stop a paused program press the [Stop] button on the control panel (see chapter 4.3) and
proceed as described in chapter 5.7.
Note: If the instrument is switched off during a pause step, this will be recognized as a power
failure. A referring error message is written to the log file and the program will be continued at
the paused step. Therefore it is necessary to stop a paused program before the instrument is
switched off.
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5.8 USB functions
By the USB port at the front side of the instrument (see chapter 4.1) programs can be
transferred from or to a USB drive.
5.8.1
Connect USB drive to Thermocycler
Plug-in the USB drive to the corresponding USB port at the front side of the instrument:
As long as no USB drive is connected the Thermocycler will try to read the memory content and
subsequently display a message: „No disk drive found“.
Note: The maximum memory size of the USB drive should not exceed more than 2 GB.
5.8.2
Access USB drive
Log-in to your user directory and press menu button [Program] (see chapter 5.1.6).
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Use the cursor keys UP and DOWN to select the directory “USB Drive”. After selecting the USB
drive the memory content is read automatically by the Thermocycler and the message „Reading
File List“ displayed.
After reading the memory content a list of programs available on the USB drive is displayed.
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5.8.3
Delete program in folder USB drive
To delete a program from the USB drive press [Delete program] (see chapter 5.8.2). In the
next screen confirm your selection with [OK] or press [Cancel] to cancel the deletion.
Note: Deleted programs cannot be restored.
5.8.4
Copy program from USB drive
To copy a program select a program file form the displayed list and press [Copy Program] (see
chapter 5.8.2). Select a storage place in your user directory and press [Save Copy].
The name of the program that is going to be copied can be changed prior storage. Press [Edit
Name] and in the next screen enter a new name for the program. Confirm with [OK] or press
[Cancel] to cancel the name change.
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5.8.5
Copy program to USB drive
To copy a program to the USB drive select a user directory and press [Copy Program].
In the next screen press [Save copy to USB].
The system displays a message with the progress in the copy process.
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After the copying is completed the new program is added to program list on the USB drive.
Note: When copying programs to the USB drive changes of the name of files will not be saved.
If you use the function [Edit name] and set a new name, the changes will not be saved when
the file is going to be stored to the USB drive [Save copy to USB]. Please save the program
with the new name to your user directory first and then use the function [Save copy to USB].
5.8.6
Maximum number of programs on the USB drive
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The maximum number of program on the USB drive is limited to 48. If this limit is exceeded a
message will be displayed.
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6
System settings
To change global settings press the [System] menu button above the display (see chapter 5.1).
The system menu main screen opens. Choose a single menu item using the cursor keys, then
press [Select].
6.1 User configuration
These functions allow changes to individual user accounts.
Choose a single menu item using the cursor keys, then press [Select] or press [Overview] to
go back to the system main menu.
6.1.1
Set language
The language can be set and stored individually for each user account. Available languages are
English and German.
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Select a language using the cursor keys and press [Select Language]. The [Default
Language] is English.
Note: The language for the boot process (before a user is logged in) can be set by the
supervisor (see chapter 6.6.4).
6.1.2
Change individual PIN
The option allows changing the PIN of the current user.
6.1.3
Delete account
Any user can delete his or her personal account.
Press [OK] to confirm the account to be deleted.
Important: To delete a user account will erase the user directory and all programs stored
within. Therefore prior to deleting an account, ensure that the user directory is empty or save a
copy of the programs to a different user directory for further usage (see section 5.4.3).
Note: Deleted user accounts and programs can not be restored!
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6.2 System configuration
The options in this menu allow to the general system settings.
Choose a single menu item using the cursor keys, then press [Select] or press [Overview] to
go back to the system main menu.
6.2.1
Configure beep
To configure the system beep, use the cursor keys to set the beep on or off and confirm
selection with softkey [Save].
The signal beep sounds when a program is going into a pause or is stopped. The signal beep
can be stopped by pressing any key (except the [Stop] button on the control panel).
The setting for [Default Beep] is beep off.
6.2.2
Configure display contrast
The display contrast can be set individually for each user.
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Change settings with the cursor keys and confirm with softkey [Save Contrast].
The setting for [Default Contrast] is the median position.
6.2.3
Set time and date
Enter settings for date and time and confirm with softkey [Set Time and Date].
6.2.4
Defragment memory
By erasing and creating PCR-protocols after a while new files may be stored in a fragmented
fashion in the memory of the TProfessional TRIO Thermocycler. This leads to increased
memory access times and the software reacts slower to user inputs. By defragmentation the
information becomes sorted and is stored in order thus leading to decreased memory access
times. Subsequently the software reacts faster to user inputs.
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Confirm your selection with [OK] and wait until the defragmentation process is finished.
6.2.5
Screen saver
Set the screen saver on or off and if necessary change the time after the screen saver is started
Press [Save] to save the settings.
The setting for [Default] is screen saver on and starts after 30 min.
6.3 System info
This menu provides information on the instrument and on the log files of the last nine runs.
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Choose a single menu item using the cursor keys, then press [Select] or press [Overview] to
go back to the system main menu.
6.3.1
System info
This function displays information for the instrument like the serial number, block type and
software version.
System Info
Hardware Info
By the function [Hardware Info] the software versions of several hardware components are
displayed. Press [System Info] or [Hardware Info] to toggle between the two windows or press
[Overview] to go back to the system main menu.
6.3.2
View run log files of the last nine runs
During each run a log file is stored in the Thermocycler memory. The log files of the last nine
runs can be viewed in this window.
Choose a program using from the list the cursor keys, then press [View Run-Logfile].
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In the run log file several information for the performed run are stored. For an overview of the
used program press [View Program], for a comprehensive of all messages for this run press
[View Messages].
The messages are listed by date and time. If a power failure takes longer than 30 minutes, a
pause step at 4°C will be started automatically as soon as the power is back. Press [View
Freeze Prog] to view the freeze step.
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6.4 Service
This menu provides several service functions for the instrument.
6.4.1
View log files of Power on self test
During the boot process the TProfessional TRIO hardware is checked (power on self test). The
results of the last log file are stored and can be displayed.
6.4.2
Execute block extended self test
In addition to the regular power on self test, a more comprehensive extended block self test can
be triggered by the user. In the extended block self test the unit can check the status and
condition of different components and functions. Therefore, this test is an important tool and
gives useful information to the customer whether the Thermocycler is still working within its
specifications.
To initiate the extended block self test press [Execute Selftest]. The test takes approximately
20 minutes. During the test no programs can be run on the thermocycler.
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At certain intervals the unit will automatically recommend to check the components of the unit,
i.e. to execute an extended block self test (message 729).
Press [Quit] to confirm that the recommendation has been noticed. In case the unit is switched
off but the extended block self test has not been initiated, the message will be shown again after
the unit is switched on.
It is recommended to execute the extended block self test regularly.
6.4.3
View log files from extended self test
The results of the extended self test log files are stored by the instrument. To view a summary
of the self test select the suitable log file from the menu using the cursor keys and press
[Select].
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The next screen summarizes the results of the extended block self test at a glance and gives an
overview for the actual status of all important system components.
Press [More results] to display additional results from the extended block self test.
Press [List Logfiles] to go back to the list of extended block self test log files.
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6.4.4
View Error log files
A history of all software and hardware errors is shown in this menu.
Note: Several errors are displayed as message screens by the software including buttons for
user interaction.
6.4.5
Create service info file for the Biometra Service Department
This option creates a service info file containing technical details for remote failure diagnosis by
the Biometra Service Department. Connect the Thermocycler by a serial cable (mode full
handshaking) to a computer and start the hyperterminal. Press [OK] to start the data transfer.
Send the SINF (Service Info File) by email to the Biometra Service Department (see chapter
6.5).
6.5 How to contact Biometra
This screen shows information how to contact Biometra.
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6.6 Supervisor system configuration
The supervisor has access to an own system menu with some specific functions.
6.6.1
Change user PIN
The supervisor can change the PIN for each user account. Select the appropriate user directory
from the list and press [Select User].
Enter a new PIN and repeat the PIN-code. Confirm with [PIN OK].
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6.6.2
Delete user account
The supervisor can delete user accounts regardless if they are password protected or not.
Select the appropriate user directory from the list and press [Select User].
Select the user account to be deleted and confirm with [OK].
The directory and all programs therein are deleted.
6.6.3
Change supervisor PIN
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The supervisor account is protected by a PIN-code. The default Supervisor PIN is 000 000 0 (do
not enter blanks). Enter your new PIN and repeat your input. Confirm with [PIN OK].
Note: Please make a note of the supervisor pin und keep it on a safe spot. A forgotten
supervisor PIN can only be reset by the Biometra Service Department or authorized Biometra
distributors.
6.6.4
Set boot language
The language displayed during the boot process can be set by the supervisor. Use the cursor
keys to select a language and confirm with [Select Language]. The [Default Language] is
English.
6.6.5
Delete all user accounts
The supervisor can delete all user accounts and including all programs. In the next screen
confirm with [OK] to delete all accounts.
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Instruction Manual TProfessional TRIO 12/2011
Note: Deleted user accounts and programs can not be restored!
Instruction Manual TProfessional TRIO 12/2011
73
7
Short manual
Safety Warnings
Do not open the instrument unless you are authorised to do so. Check the
label at the backside if the instrument has correct voltage configuration (110V,
115 V or 230 V, see chapter 3.4).
The thermoblock and the heated lid will reach high temperatures during
operation. Both thermoblock and heated lid can burn you.
Rapid heating of the thermoblock can cause liquids to boil explosively. Always
wear safety goggles during operation. Close the lid before starting a program.
It is not necessary to apply oil into the opening of the block in order to improve
the heat transfer between the block and the sample tubes. If you still decide to
use oil, do not use silicon oil. Mineral oil may be used.
Ensure that both the rear and bottom ventilation slits at the rear and bottom of
the instrument are unobstructed. Insufficient ventilation can cause overheating
of the instrument.
The TProfessional TRIO Control Panel
Log in
After the TProfessional TRIO has passed the initial self-diagnosis the login menu is displayed:
 Press [Log in] and log in as existing user
 Press [Supervisor Log in] and log in as supervisor
 Press [New User] and create new user account
The language displayed in the user interface can be selected (English or German). The selected
language will be stored for each individual user.
 Press [Select Language] to select preferred language
 To set boot language log in as supervisor and select boot language in the systems menu. Press
[Select Language] to set boot language.
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Instruction Manual TProfessional TRIO 12/2011
Create, modify and save programs
 To create a new or edit an existing program, press the [Program] menu button.
 Move cursor to your directory and press [Open directory].
 Press [New Program] or choose existing program and press [Edit Program] to enter the program
screen.
Use the cursor keys to navigate in the program screen. In each case the activated field is displayed
inverted. Corresponding to the activated field the assignment of the softkeys in the TProfessional TRIO
control panel will change accordingly.
Note: In the directory “Biometra Shared” protocol templates for different PCR applications are preinstalled. In chapter 5.3.1 instructions are given how to use these protocols.
The TProfessional TRIO programming spreadsheet
 To switch lid preheating on or off activate the corresponding status display and press [Preheat
ON/OFF].
Set lid temperature in status display [-- °C] within the range of 30°C to 99°C.
 Enter temperature [°C] and time [m:s] in the programming table for each single step. To hold a
temperature for an indefinite time enter “0” in column [m:s] and press <enter>. Subsequently, the
word “Pause” is shown in the display.
Note: Minutes and seconds can be separated by “dot” or digits can be entered one after the other.
Example: To set 2 minutes, 30 seconds enter “2”, “•”, “3”, “0” or “2”, “3”, “0”.
 To insert or delete a program step move cursor to the corresponding position in the programming table
and press [Insert/Delete Step].
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75
 To insert or delete a program step press [Insert Step] or [Delete Step]. The position at which a
step will be inserted or deleted is highlighted.
 In the next screen confirm insertion or deletion with [OK].
 To define a loop a) enter the step the program jumps go back to [goto] and b) enter how often the
loop is repeated [loops]. The defined loop is graphically displayed by a bracket at the left side of the
programming spreadsheet, the total cycle number is mentioned.
 To program special features like temperature increment, time increment or heating and cooling rate
repeatedly press the cursor key <right> to open the options tab. A new table is displayed:
Tip:
Storing samples at 16°C rather than 4°C at the
end of a run will increase the instruments life
time.
 To reduce or increase the annealing temperature from step to step within a loop set a negative or
positive temperature increment in the column ∆T(°C).
 To increase or reduce the incubation time from step to step in a loop set a positive or negative time
increment in the column ∆t(s).
 The ramping rate (heating and cooling) is pre-set according to the block type To adjust the ramping
rate enter any value in steps of 0.1°C in the column Ê(°C/s).
 To define protocols including a gradient-like temperature optimisation step enter two temperatures for
block 1 and block 3 separated by a minus in the column [°C] (e.g. 50-60) or press [TempOpti]. This
activates the screen for programming temperature optimisation steps:
 Enter an annealing temperature and an increment. The annealing temperature defines the
temperature for block 2. The increment defines the temperatures for block 1 and block 3. The resulting
temperatures for block 1-3 are shown below.
Note: To start programs containing a temperature optimisation step all three blocks are required.
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Instruction Manual TProfessional TRIO 12/2011
 Press [Save/Save as] to save a program.
Subsequently the program storage screen opens:
Tip:
A folder with common protocols could be
established. From the common folder each user
may copy any program into the own account and
modify it easily.
 By default the program is stored at the first free storage place available. To save a program at a
specific storage place use cursor keys <up> and <down> or enter two digits.
 To edit the name of the program press [Edit name]. In the next screen enter a name by using the
keypad and confirm with [OK].
Start, pause and stop programs
 The TProfessional TRIO Thermocycler for each user stores the last 5 programs that have been run or
modified. For quick start of one of these programs press the [Block] menu button. In the next screen
select one of the last five programs by using the cursor keys <up> and <down>. Press the [Start]
button on the left side of the keypad to start the program.
 To start a program from a user directory log in to your user account and press the menu button
[Program]. In the next screen select the appropriate user directory and program by navigating with the
cursor keys. Press the [Start] button on the left side of the control panel to start the selected program.
After starting a program the following screen is shown:
 To pause an active, program select the corresponding block and press [Block 1], [Block 2] or [Block
3]. In the next screen press [Pause Block/Skip Step]. The message “Pause” is shown in the display
for the paused block.
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77
 To continue a paused program select the corresponding block and press [Block 1], [Block 2] or
[Block 3]. In the next screen press [Continue].
 To stop an active program press the [Stop] button on the control panel. Select a single block [Stop
Block 1], [Stop Block 2] or [Stop Block 3] or select all blocks [Stop all blocks] to be stopped. In the
next screen confirm with [OK].
 Programs with a pause in the last step have to be stopped manually as well.
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Instruction Manual TProfessional TRIO 12/2011
8 Trouble shooting
8.1 Slow heating and cooling
The TProfessional TRIO is equipped with a strong ventilator for the cooling of the heat sink. The
inlet of this fan is located at the bottom side of the instrument. Be sure that the inlet is not
clogged by dust or other material (e.g. a sheet of paper placed under the cycler can be attached
to the inlet as the fan is in operation). Dust can be removed easily from the inlet with a
conventional vacuum cleaner.
8.2 Autorestart
Running programs will be continued from the same step where the power failure has happened
as soon as the power is supplied again. A message is displayed showing (a) that a power
failure occurred during the run and (b) when and at which step the program was started again:
Confirm with [OK] to close the message screen.
8.3 Restart due to unrecognised power failure
High voltage fluctuation can lead to an automatic restart of the Thermocycler. In this case the
cycler restarts at the step where there power failure has occurred. To avoid voltage fluctuation,
do not connect the cycler to a socket shared by a strong power consumer like a refrigerator or a
centrifuge.
8.4 Adaptation of protocols from other cyclers
Since the TProfessional TRIO is a fast instrument it may be necessary to reduce the heating
and cooling ramps to run protocols from other cyclers. For the setting of the heating and cooling
ramps see section 5.5.1. Alternatively, the time settings may be extended.
8.5 Releasing wheel in case of blocked lid
When the lid is in the very up or down position, it may happen that the wheel is uncoupled. In
this situation the clutch mechanism is active in both directions (clicking noise in either direction).
Instruction Manual TProfessional TRIO 12/2011
79
To unlock wheel, press down metal pin with a ball pen and turn wheel carefully. This pin
overrides the automatic clutch mechanism. Thus, care must be taken not to apply excessive
pressure.
Release lid in upper position:
1) press pin
2) carefully turn wheel while holding
the pin down CLOCKWISE, until you
feel normal resistance (no more
clicking noise, clutch is released).
Release pin and turn lid down, until
the clutch mechanism is activated
(clicking noise, optimum pressure
applied).
1
2
Release lid in down position:
1) press pin
2) carefully turn wheel while holding
the pin down COUNTER
CLOCKWISE, until you feel normal
resistance (no more clicking noise,
clutch is released). Release pin and
turn wheel counter clockwise until
pressure is completely released.
Open lid.
Important: When the clutch mechanism is active (= optimum pressure is applied), do not use pin
to further increase lid pressure. This would lead to damage of tubes and instrument!
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Instruction Manual TProfessional TRIO 12/2011
9 Maintenance and repair
9.1 Cleaning and Maintenance
The TProfessional TRIO was built to operate for a long time without the need for periodical
maintenance. Nevertheless, occasionally cleaning of the air inlet may be necessary to maintain
the efficiency of the Thermocycler. Insufficient airflow may lead to reduced heating and cooling
rates. The inlet for the airflow is located at the bottom side of the instrument. Be sure that the
inlet is not clogged by dust or other material (e.g. a sheet of paper placed under the cycler can
be attached to the inlet as the fan is in operation). Dust can be removed easily from the inlet
with a conventional vacuum cleaner. Additionally, the Thermocycler housing may be cleaned
from time to time with a smooth cotton cloth. Do not use strong detergents or organic solvents
for cleaning. Never treat silver block with abrasive agents.
Note: Appropriate safety regulations must be observed when working with infectious or
pathogenic material.
9.2 Servicing and repair
The TProfessional TRIO Thermocycler contains no user serviceable parts. Do not open the
housing instrument. Service and repair may only be carried out by the Biometra Service
department or otherwise qualified technical personal.
The Service department offers Thermocycler maintenances and temperature verifications.
Biometra recommends an annual maintenance and a biannual temperature check for all
Thermocyclers. Please call the following phone number for detailed information: +49 55150881-10/12.
9.3 Firmware update
For instruction for firmware upgrade, please contact the Biometra Service Department or your
local distributor/sales representative.
9.4 Replacement of spare parts
Only original spare parts mentioned in these operating instructions are allowed.
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81
10 Accessories
10.1 Plasticware
Biometra offers a broad range of plasticware for the use in PCR. The following table provides an
overview for the product portfolio:
Cat. no.
82
050-310
050-320
Description
Single tubes
0.2 ml tubes with caps
0.5 ml tubes with caps
Quantity
1000 pcs.
1000 pcs.
050-254
050-255
Tube strips
Strips 8 tubes and flat caps
Strips 8 tubes and domed caps
125 pcs.
125 pcs.
050-225
48 well plates
48 well microplate
50 pcs.
050-232
050-213
050-253
96 well plates
96 well skirted
96 well non-skirted (low profile)
96 well non-skirted
25 pcs.
25 pcs.
25 pcs.
050-231
050-240
384 well plates
HSQ 384 well skirted
384 well microplate
50 pcs.
50 pcs.
050-237
050-256
050-257
050-236
050-194
050-694
050-251
050-252
Miscellaneous
Silcone mat
Adhesive film
Heat Sealing Film
Heat Sealing Film Aluminium
96 well aluminium plate
384 well aluminium plate
Nop mat 96 well
Nop mat 384 well
50 pcs.
100 pcs.
100 pcs.
10 pcs.
1 pcs.
1 pcs.
20 pcs.
20 pcs.
Instruction Manual TProfessional TRIO 12/2011
11 Service
Should you have any problems with this unit, please contact our service department or your
local Biometra distributor:
Biometra GmbH
Service Department
Rudolf-Wissell-Straße 14 - 16
D-37079 Göttingen
Phone:++49 (0)5 51 50 68 6 - 10 or 12
Fax: ++49 (0)5 51 50 68 6 -11
e-mail: [email protected]
If you would like to send the unit back to us, please read the following return
instructions in chapter 11.1.
11.1 Instructions for return shipment
In case of an instrument failure that cannot be fixed by the procedures described in section 8
please proceed as follows:
•
Return only defective devices. For technical problems which are not definitively recognisable
as device faults please contact the Technical Service Department at Biometra (Tel.: +49 55150881-10/12, Fax: +49 551-50881-11, e-mail: [email protected]).
•
Please contact our service department for providing a return authorization number (RAN).
This number has to be applied clearly visible to the outer box. Returns without the RAN will
be not be accepted!
•
Important: Carefully clean all parts of the instrument of biologically dangerous, chemical or
radioactive contaminants. If an instrument is contaminated, Biometra will be forced to refuse
to accept the device. The sender of the repair order will be held liable for possible losses
resulting from insufficient decontamination of the device.
•
Please prepare written confirmation that the device is free from biologically dangerous and
radioactive contaminants. The declaration of decontamination (see section 12) must be
attached to the outside of the packaging.
•
Use the original packing material. If not available, contact Biometra or your local distributor.
•
Label the outside of the box with “CAUTION! SENSITIVE ELECTRONIC INSTRUMENT!”
•
Please enclose a note which contains the following:
a)
Sender’s name and address,
b)
Name of a contact person for further inquiries with telephone number,
c)
Description of the fault, which also reveals during which procedures the fault occurred, if
possible
Instruction Manual TProfessional TRIO 12/2011
83
11.2 Packing of the Thermocycler
Biometra uses an extra designed packaging system where the instrument is mounted in
between two tearproof foils. The Thermocycler is put onto the lower inlet and is fixed in between
the foils by pressing the upper inlet down.
Note: The Thermocycler is only protected from transport damage if the packing instructions are
followed and the instrument is mounted in between the foils. Biometra will not be responsible for
transport damage by improper packing.
Gerät zwischen den
Folien schwebend
einspannen!
Mount instrument
floating in between
the foils!
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Instruction Manual TProfessional TRIO 12/2011
12 Equipment Decontamination Certificate
To enable us to comply with german law (i.e. §71 StrlSchV, §17 GefStoffV and §19 ChemG)
and to avoid exposure to hazardous materials during handling or repair, please complete this
form, prior to the equipment leaving your laboratory.
COMPANY / INSTITUTE
__________________________________________________
ADDRESS
______________________________________________________________
PHONE NO
_________________________
E-MAIL
______________________________________________________________
EQUIPMENT
FAX NO_________________________
Model
______________
Serial No
______________
______________
______________
______________
______________
______________
______________
If on loan / evaluation Start Date: __________________
Finish Date __________________
Hazardous materials used with this equipment:
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
Method of cleaning / decontamination:
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
The equipment has been cleaned and decontaminated:
NAME __________________________________ POSITION _______________________
(HEAD OF DIV./ DEP./ INSTITUTE / COMPANY)
SIGNED ________________________________ DATE ____________________________
PLEASE RETURN THIS FORM TO BIOMETRA GMBH OR YOUR LOCAL BIOMETRA
DISTRIBUTOR TOGETHER WITH THE EQUIPMENT.
PLEASE ATTACH THIS CERTIFICATE OUTSIDE THE PACKAGING. INSTRUMENTS
WITHOUT THIS CERTIFICATE ATTACHED WILL BE RETURNED TO SENDER.
Instruction Manual TProfessional TRIO 12/2011
85
General Information for Decontamination:
Please contact your responsible health & safety officer for details.
Use of radioactive substances:
Please contact your responsible person for details.
Use of genetically change organism or parts of those:
Please contact your responsible person for details.
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Instruction Manual TProfessional TRIO 12/2011
13 Note for the disposal of electric / electronic waste.
Note
for disposal of electric / electronic waste
Hinweis
für die Entsorgung von Elektroaltgeräten
Renseignement
du traitement des déchets des appareils
électrique / électronique
This symbol (the crossed-out wheelie bin) means, that this product should be brought to the
return and / or separate systems available to end-users according to yours country regulations,
when this product has reached the end of its lifetime.
For details, please contact your local distributor!
This symbol applies only to the countries within the EEA*.
EEA = European Economics Area, comprising all EU-members plus Norway, Iceland and
Liechtenstein.
-------------------------------------------------------------------------------------------------------------------------------------------Dieses Symbol (die durchgestrichene Abfalltonne) bedeutet, dass dieses Produkt von der Firma
Biometra für eine kostenlose Entsorgung zurückgenommen wird. Dies gilt nur für Geräte, die
innerhalb Deutschlands gekauft worden sind.
Kontaktieren Sie für die Entsorgung bitte die Biometra Service-Abteilung!
Außerhalb Deutschlands wenden Sie sich bitte an den lokalen Händler.
Dieses Symbol gilt nur in Staaten des EWR*.
*EWR = Europäischer Wirtschaftsraum, umfasst die EU-Mitgliedsstaaten sowie Norwegen, Island
und Liechtenstein.
-------------------------------------------------------------------------------------------------------------------------------------------Cet symbol (conteneur à déchets barré d´une croix) signifie que le produit, en fin de vie, doit être
retourné à un des systèmes de collecte mis à la disposition des utilisateurs finaux en
conséquence des régulations par la loi de votre pays.
Pour des information additionel nous Vous demandons de contacter votre distributeur!
Cet symbole s´ápplique uniquement aux pays de l´EEE*.
EEE = Espace économique européen, qui regroupe les États membres de l´UE et la Norvège,
Islande et le Liechtenstein.
Instruction Manual TProfessional TRIO 12/2011
87
14 EU – Konformitätserklärung / EU - Declaration of
Conformity
EU – Konformitätserklärung
EC - Declaration of Conformity
Göttingen, August 2011
im Sinne der EG-Richtlinie über elektrische Betriebsmittel zur Verwendung innerhalb
bestimmter Spannungsgrenzen 2006/95/EG
following the EC directive about electrical equipment for use within certain limits of voltage
2006/95/EC
und / and
im Sinne der EG-Richtlinie für die elektromagnetische Verträglichkeit 2004/108/EG.
following the EC directive about the electromagnetic compatibility 204/108/EC.
Hiermit erklären wir, dass folgende Thermocycler:
Herewith we declare that the following Thermocyclers:
Typen / types:
Best.-Nr. / Order No.:
TProfessional TRIO 30, TProfessional TRIO 48,
TProfessional TRIO combi
070-720, 070-723, 070-724
den grundlegenden Anforderungen der
corresponds to the basic requirements of
EG-Niederspannungsrichtlinie 2006/95/EG und der
EC low voltage directive 2006/95/EC and the
EG-Richtlinie über die elektromagnetische Verträglichkeit 2004/108/EG entsprechen.
EC directive about the electromagnetic compatibility 204/108/EC.
Folgende harmonisierte Normen wurden angewandt:
The following harmonized standards have been used:
EN61326
EN61000-3-2
EN61000-3-3
EN61010-1
EN61010-2-010
Dr. Jürgen Otte
Quality Manager
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Instruction Manual TProfessional TRIO 12/2011
15 Warranty
This Biometra instrument has been carefully build, inspected and quality controlled before
dispatch. Hereby Biometra warrants that this instrument conforms to the specifications given in
this manual. This warranty covers defects in materials or workmanship as described under the
following conditions:
This warranty is valid for 24 months from date of shipment to the customer from Biometra. This
warranty will not be extended to a third party without a written agreement of Biometra.
This warranty covers only the instrument and all original accessories delivered with the
instrument. This warranty is valid only if the instrument is operated as described in the manual.
Biometra will repair or replace each part which is returned and found to be defective. This
warranty does not apply to wear from normal use, failure to follow operating instructions,
negligence or to parts altered or abused.
Instruction Manual TProfessional TRIO 12/2011
89
16 Subject Index
Autorestart ............................................... 79
Beep......................................................... 61
Block menu .............................................. 20
Contact..................................................... 69
Declaration of Conformity ........................ 88
Decontamination Certificate..................... 85
Defragmentation ...................................... 62
Display
Contrast ............................................... 61
Disposal ................................................... 87
Firmware update ...................................... 81
Heated lid........................................... 26, 27
Heated Lid................................................ 79
Konformitätserklärung.............................. 88
Language ........................................... 59, 72
Log file ......................................... 66, 67, 69
Log in ....................................................... 22
Log in menu ............................................. 20
Maintenance ............................................ 81
Operation Voltage .................................... 14
Packing .................................................... 84
PIN-Code ............................... 23, 60, 70, 71
Plasticware............................................... 82
Program
continue ............................................... 50
cooling rate .......................................... 41
copy ..................................................... 37
Create .................................................. 25
delete ................................................... 38
delete step ........................................... 36
edit ....................................................... 34
graphical mode .................................... 40
heating rate .......................................... 41
insert step ............................................ 34
loop ...................................................... 28
pause ............................................. 29, 49
quick start............................................. 45
save ..................................................... 29
skip step............................................... 50
90
spread sheet mode ...............................40
start.......................................................43
stop .......................................................52
temperature increment..........................42
time increment ......................................42
Program menu ..........................................21
Repair .......................................................81
Safety precautions ....................................10
Screen saver ............................................63
Self test
extended ...............................................66
Power on self test .................................66
Service......................................................66
Service info file .........................................69
Servicing ...................................................81
Short manual ............................................74
Start ..........................................................43
Supervisor ..........................................24, 70
System configuration ................................61
System info ...............................................63
System menu............................................21
System settings ........................................59
Technical specifications..............................8
Temperature
optimisation...........................................32
Time and Date ..........................................62
USB
copy program..................................55, 56
delete program......................................55
functions ...............................................53
max. program number ..........................57
open folder............................................53
User account
create....................................................24
delete ........................................25, 71, 72
User Account
Delete ...................................................60
User interface ...........................................19
Warranty ...................................................89
Instruction Manual TProfessional TRIO 12/2011
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