Standard Power Pack P25 Manual

Standard Power Pack P25 Manual
Standard Power Pack P25
Order-No. 040-800
Manual
!!Attention!!
Please read this manual carefully
prior to operating the device
Biometra GmbH
Rudolf-Wissell-Straße 30, D-37079 Göttingen
P.O. Box 1544, D-37005 Göttingen
Phone: ++49 – (0)5 51 / 50 68 6-0
Fax:
++49 – (0)5 51 / 50 68 6-66
E-mail: [email protected]
Internet: http://www.biometra.com
Service Division
Rudolf-Wissell-Straße 14-16, D-37079 Göttingen
Phone: ++49 – (0)5 51 / 50 68 6-10 or -12
Fax:
++49 – (0)5 51 / 50 68 6-11
E-mail: [email protected]
1
This document describes the state at the time of
publishing. It needs not necessarily agree with future versions.
Subject to change!
Standard Power Pack P25 Manual, 2010/11
1.0 Intended Uses and Specification
The Biometra Standard Power Pack P25 is suitable for
PAGE, SDS-PAGE, agarose gel electrophoresis, blotting and
electroelution of proteins and DNA/RNA from gels.
The Standard Power Pack P25 is intended for use with any
electrophoresis applications designed to operate below 400
volts and 500 milliamps DC (electrophoresis) or 200 volts and
1000 milliamps DC (blotting). Four sets of outputs operate in
parallel to provide 0 to 400 volts, up to 1000 milliamps, and up
to 200 watts in constant voltage or constant current mode.
Voltage and current setting is adjustable in steps of 1V and
1mA.
A single LCD display provides output voltage or current
readings.
0
2.0 Technical Data
Type Output:
Max. Voltage:
Max. Current:1
Max. Power:
Const. Voltage and Const. Current
400 VDC
1000mA in the range 0-200VDC
<1000mA in the range 200-400VDC
200 W, continuous
Output Display:
LCD-display, 3.5 digits, selectable
between display mA, and V
Resolution 1 mA and 1 V, respectively
Accuracy:
+- 5% v.M.
(for U, I >= 0,05 * Umax, Imax)
+- 5% of 0,05 * Umax, Imax
(for U,I < 0,05 * Umax, Imax)
Arithmetic mean over 3 s, Ts = 0.4 s
Number of Output
Terminals:
Four sets of 4 mm safety-sockets
Input Power:
Allowable deviation:
100–240VAC/50-60Hz/max. 300W
+6/-10%
Ambient Operating
Temperature Range:
0 to 40°C
Humidity:
0 to 70%
Dimensions:
(W x L x H) 26.5 x 25.0 x 9.5 cm
Weight:
3 kg
The Standard Power Pack P25 is designed for use with AC
net supply voltages between 100 Volt and 240 Volt, 50-60Hz.
It is a switched mode power supply providing voltage and
current controlled DC power, respectively. The DC output is
free of ground and short-circuit proof.
1
See also chapter 5.7
1
3.0 Safety Instructions
The Standard Power Pack P25 must only be used in
accordance with its designation. The use of this device
unconformably to the operating instructions will dissolve any
responsibility and liability.
Observe the relevant rules for accident prevention
as well as those of approved safety and
occupational medicine!
Do not attempt any repair yourself. In need of
repair, consult your local qualified service
representative. Only qualified personnel may open
the housing parts. The inner device may still
contain dangerous voltage several minutes after
disconnection from power supply.
Make sure the indicated voltage and frequency
range of the device corresponds with the system
voltage and that the power switch is on “0” before
it is connected to the power supply.
Connect the device to a properly grounded outlet
only!
Pull the power cord out of the outlet to safely
disconnect from the power supply.
2
Use exclusively the provided power cord or one
that meets the legal requirements.
Pay attention to the dimensioning of the fuses and
replace them if required. The fuse holder is located
in the lower part of the power connector on the
back side of the Power Pack. The following fuses
are required:
Micro-fuses, slow blow
2 x 5A; 250 V
"Caution: For continued protection against risk of
fire, replace only with same type and rating of
fuse".
"Caution: Double pole/neutral fusing, disconnect
power before changing fuse"
In order to ensure the safe electrical disconnection
the floating outputs must never be grounded.
Make sure that your test setup always complies to
this condition.
Only safe electrical cords certified according to
IEC / EN 61010-2-031 may be connected to the
power outputs.
Make sure the Standard Power Pack P25 is
located in a safe and dry place, in proximity to the
electrophoresis devices powered. Keep away from
liquids. Should a liquid penetrate the P25,
disconnect the device immediately.
3
Do not operate in a damp or humid environment
where condensing moisture may lead to creeping
current. See chapter 2 (Technical Data) for
ambient operating temperature range.
When moving from a cold to a warm room, allow 8
hours before reusing the device in order for it to
reach the ambient temperature and for possible
condensation to evaporate.
Use only mild, non-abrasive detergents. Carefully
clean the device to avoid liquids penetrating the
inner housing.
When positioning the P25, make sure the
ventilation openings on the back side are not
covered.
Should the Power Pack become extremely warm,
smoke or display unusual information while in
operation, discontinue use immediately and
contact a qualified service representative.
4
4.0 Set Up
4.1 Content
•
•
•
Standard Power Pack P25
Manual
Power Cord
4.2 Unpack and Check
Your Biometra Standard Power Pack P25 has been carefully
packed to ensure its safety. Please unpack it carefully, control
the integrity of its content and inspect the device. Report any
damage immediately to BIOMETRA!
Do not operate a device which is in no proper condition.
Should you have a complaint, return the complete system in
its original packing by following the return instructions (see
chapter 8.0).
!!Attention!!
Please send back the enclosed postcard along with
the filled out Warranty Certificate to Biometra in
order to claim the full warranty!
5
4.3 Power Supply
Before connecting, make sure the actual voltage complies with
the voltage range indicated on the type label.
Caution:
The Standard Power Pack P25 must be
grounded!
4.4 Location
The Standard Power Pack P25 must be located in a dry and
safe place. Do not place an electrophoresis chamber on the
power supply device. The ventilation openings on the back
side must not be covered by other devices, furniture or
fixtures. A sufficient air flow for convection cooling must be
provided.
4.5 Electrophoresis Chamber Connection
The electrophoresis chamber is connected to the Standard
Power Pack P25 by safety cords. Use exclusively
electrophoresis devices with safety connectors.
Switch off the Standard Power Pack P25 while
working on the test equipment setup.
6
5.0 Operation
5.1 Power On
Establish the connection to the electrophoresis chamber(s)
before switching on the Standard Power Pack P25. Then
switch on the device using the power switch. A green light
appears over the power switch when power is turned on.
When power is turned on, the P25 sets the output
voltage or current to the latest value stored.
Should you be unsure of the latest saved settings, detach all
connections to the electrophoresis chamber and switch on the
Standard Power Pack P25 in order to check the voltage or
current settings (see chapter 5.2 Voltage Setting and chapter
5.3 Current Setting).
The device must always be turned off before the
supply line to the electrophoresis chamber is to be
connected or removed for any given reason (power
switch on “0” and LED not illuminated).
5.2 Voltage Setting
By using the “SELECT” button, switch to “VOLT” on the
display. Set the desired output voltage by turning the knob.
In normal mode, the display shows the output voltage (actual
value). By turning the knob, the display changes from the
actual value to the desired set value, recognizable by the
flashing “VOLT” LED indicator. The desired value increases
with every clockwise rotation and decreases with every
counter clockwise rotation. Depending on speed and direction
of the rotation, the value will augment or reduce in steps of 1V,
7
10V or 100V. A set value modified by the user will, 3 seconds
after the last operation of the knob, precede all other values
and will be set as the desired output voltage.
The voltage value (V) should be set without any
device connected!
5.3 Current Setting
By using the “SELECT” button, switch to “mA” on the display.
Set the desired output current by turning the knob.
In normal mode, the display shows the current conduction
(actual value). By turning the knob, the display changes from
the actual value to the desired set value, recognizable by the
blinking “mA” LED indicator. The desired value increases with
every clockwise rotation and decreases with every counter
clockwise rotation. Depending on speed and direction of the
rotation, the value will augment or reduce in steps of 1mA,
10mA or 100mA. A set value modified by the user will, 3
seconds after the last operation of the knob, precede all other
values and will be set as the desired output current.
The electric current value (mA) should be set
without any device connected!
8
5.4 Monitoring Voltage and Current
5.4.1 Monitoring Set Values
Choose the desired measurement category by using the
“SELECT” button in order to monitor the default voltage and
current values. Then turn the knob to the right or to the left by
one detent and the LCD displays will show the desired set
value of the corresponding measurement. The value
appearing on the display will, 3 seconds after the last
operation of the knob, precede all other values. The LCD
display will then automatically be switched to the actual value.
5.4.2 Monitoring Actual Values
Choose the desired measurement category at any time by
using the “SELECT” button in order to control the output
voltage and current. In doing so, only the LCD display will be
switched. The supplied current or applied voltage will not be
affected by this operation.
5.5 Working with Constant Voltage
Adjust the voltage controller’s set value to the desired output
voltage (see chapter 5.2 Voltage Setting) when working with
constant voltage. Then select the maximum possible or
allowable value for the current controller’s set value. The
current controller’s set value will then be the maximum
possible or allowable value chosen (see chapter 5.3 Current
Setting).
The Standard Power Pack P25 supplies a constant output
voltage until reaching the selected current limit (voltage
control). The output current then varies according to the ohmic
resistance of the electrophoresis chambers. The output of a
9
constant voltage will be shown by the LED indicator
“VOLTAGE” (see chapter 6.3 Operating Status Display).
Should the maximum allowable output current be exceeded,
the Standard Power Pack P25 will limit the current and the
output voltage will drop.
Application example:
An agarose gel electrophoresis should be conducted with a
constant voltage of 150V. For safety reasons the output
current should not exceed 300mA. The following set values
will be applied:
Output voltage:
Output current:
150V
300mA
10
5.6 Working with Constant Current
Adjust the current controller’s set value to the desired output
current (see chapter 5.3 Current Setting) when working with
constant current. The voltage controller’s set value will then
have been selected so high that the device will switch to
current limit mode (see chapter 5.2 Voltage Setting).
When the current output has reached the desired set value,
the Standard Power Pack P25 will limit the current. The
voltage output will then adjust to the ohmic resistance of the
electrophoresis chamber and the current output will remain
constant.
The LED indicator “CURRENT” (see chapter 6.3 Operating
Status Display) is illuminated throughout the current limitation
mode.
Should the set value for the voltage controller be
selected too low, the output voltage will therefore not
suffice to reach the desired current.
The output power of the Standard Power Pack P25 is
limited to 200W. The maximum allowable current
output of the device is limited to 200V as shown in
figure 1 of chapter 5.7.
Application example:
A semi-dry blot should be conducted with a constant current of
800 mA. For safety reasons the output voltage should not
exceed 50V. The following set values will be applied:
Output voltage:
Output current:
50V
800mA
11
5.7 Power Limitation
The output power of the Standard Power Pack P25 is limited
to 200W. Should the user’s selected voltage and current
defaults exceed the maximum allowable output power, the
device will reduce the default set value of the output current as
shown in figure 1.
Output Current
1A
Power
Limitation
0,5 A
0A
0V
200 V
400 V
Output Voltage
Figure 1:
Automatic limitation of the output current at high
output voltage values
The “CURRENT” and “VOLTAGE” LED indicators are both
illuminated when the current limitation is accessed (see
chapter 6.3 Operating Status Display) to give a signal that the
output current, due to the “power limitation”, is lower than
the value originally set by the user.
12
5.8 Switching Off
The Standard Power Pack P25 can be turned on or off by
pressing the on/off switch.
The device must always be turned off before the
supply line to the electrophoresis chamber is to be
connected or removed for any given reason (power
switch on “0” and LED above not illuminated).
13
6.0 Status Notification
6.1 Power ON LED
A green LED located above the on/off switch shows, along
with the on/off switch position, if the Standard Power Pack
P25 works properly.
on/off switch
LED
Meaning
position
indication
“O”
dark
No error:
Power off
“I”
green
No error:
Power on
“I”
dark
Error:
• No connection to power
• Faulty fuse
• Device failure
The Power On LED remains dark although the device is
switched on when there is an operation or hardware failure. In
such case, please verify that
•
•
•
the power outlet supplies electricity,
the Standard Power Pack P25 is correctly connected
to the power source,
the fuses in the power inlet socket are not defective.
Caution: Disconnect the device from the power
supply by unplugging the power cord before any
fuse replacement!!!
14
6.2 Display Indication
The actual and set values for the voltage and current outputs
will alternatively be shown on the LCD display (see chapter
5.4 Monitoring Voltage and Current). The two LEDs on the
right side of the display indicate to which parameter the value
shown on the display belongs. The following rules apply:
LED indication
“mA” – LED illuminated
“VOLT” – LED illuminated
“mA” – LED flashes
“VOLT” – LED flashes
Display indication
Output current in mA
Output voltage in volt
Set value for the current
control in mA
Set value for the voltage
control in volts
15
6.3 Operating Status Display
The Standard Power Pack P25 is in one of the following three
operating modes “voltage limitation”, “current limitation” and
“power limitation” depending on the selected voltage and
current settings and the ohmic resistance of the
electrophoresis chamber. The operating mode is indicated by
the two LEDs located below the display. The following rules
apply:
LED indication
Display indication
“CURRENT” – LED
Current limitation
illuminated
“VOLTAGE” – LED illuminated Voltage limitation
“CURRENT” – LED and
Power limitation
“VOLTAGE” – LED illuminated
In the “voltage control” mode the output voltage will remain
equal to the selected value (see also chapter 5.5 Working with
Constant Voltage).
Should the output current exceed the selected default value,
the “current limitation” function of the Standard Power Pack
P25 is activated. The output voltage is now adjusted to the
ohmic resistance of the electrophoresis chamber. The output
current remains limited to the selected default value (see also
chapter 5.6 Working with Constant Current).
Should the user’s selected voltage and current defaults
exceed the maximum allowable output power of 200W, the
device will reduce the maximum allowable output current
according to figure 1 in chapter 5.7. Both LED indicators are
illuminated when the current limitation is accessed to indicate
that the output current, due to the “power limitation”, is lower
than the value originally set by the user.
16
7.0 Cleaning
Disconnect the Power Pack. Use only mild, non-abrasive
detergents and a slightly moistened soft cloth. Do not use
solvents, soak while cleaning or immerse in water!
17
8.0 Service
Should you have any problem with the device, please contact
our service division or your local Biometra dealer:
Biometra
GmbH
Service Division
Rudolf-Wissell-Straße 14-16, D-37079 Göttingen
Phone: ++49 – (0)5 51 / 50 68 6-10 or -12
Fax:
++49 – (0)5 51 / 50 68 6-11
E-mail: [email protected]
Please follow the return instructions when returning the
device:
Instructions for return shipment
•
Return only defective devices. For technical problems which are
not definitively recognisable as device faults please contact the
Technical Service Department at Biometra
(Tel.: +49 (0)5 51-50 88 1-10 or -12, Fax: +49 (0)5 51-50 88 1-11,
e-mail: [email protected]).
•
•
Please contact our service department for providing a return authorization number (RAN). This number has to be
applied clearly visible to the outer box. Returns without
the RAN will be not be accepted!
Important: Carefully clean all parts of the instrument
from residues, and of biologically dangerous,
chemical or radioactive contaminants. If an
instrument is contaminated, Biometra will be
18
forced to refuse to accept the device. The sender of the repair order will be held liable for possible damages and
losses resulting from insufficient decontamination of the
device.
•
•
•
•
Please prepare written confirmation (use the “Equipment Decontamination Declaration” following on the next
page) that the device is free of biologically dangerous,
chemical or radioactive contaminants. This confirmation
must be attached to the outside of the packaging.
Use the original packing or a similarly robust packing when
returning the device. If not available, contact Biometra or
your local distributor.
Label the outside of the box with “CAUTION! SENSITIVE
INSTRUMENT!” and the RAN number sticker. Attach the
Decontamination Declarartion!
Please enclose a note which contains the following:
a) Sender’s name and address,
b) Name of a contact person for further inquiries with
telephone number.
c) Precise description of the fault, which also reveals
during which procedures the fault occurred, if possible.
19
9.0 Equipment Decontamination Certificate
To enable us to comply with german law (i.e. §71 StrlSchV, §17 GefStoffV
and §19 ChemG) and to avoid exposure to hazardous
materials during handling or repair, please complete this form, prior
to the equipment leaving your laboratory.
COMPANY / INSTITUTE ___________________________________
ADDRESS ______________________________________________
PHONE NO _______________
FAX NO __________________
E-MAIL ________________________________________________
EQUIPMENT
Model
______________
Serial No
______________
______________
______________
______________
______________
If on loan / evaluation Start Date: ________ Finish Date _________
Hazardous materials used with this equipment:
_______________________________________________________
_______________________________________________________
_______________________________________________________
Method of cleaning / decontamination:
_______________________________________________________
_______________________________________________________
_______________________________________________________
The equipment has been cleaned and decontaminated:
NAME _____________________ POSITION _________________
(HEAD OF DIV./ DEP./ INSTITUTE / COMPANY)
SIGNED ____________________ DATE _____________________
PLEASE RETURN THIS FORM TO BIOMETRA GMBH OR YOUR LOCAL
BIOMETRA DISTRIBUTOR TOGETHER WITH THE EQUIPMENT.
PLEASE ATTACH THIS CERTIFICATE OUTSIDE THE PACKAGING.
INSTRUMENTS WITHOUT THIS CERTIFICATE ATTACHED WILL BE
RETURNED TO SENDER.
20
General Information for Decontamination:
Please contact your responsible health & safety officer for
details.
Use of radioactive substances:
Please contact your responsible person for details.
Use of genetically change organism or parts of those:
Please contact your responsible person for details.
21
10.0 Note for Disposal of
Eletric/Electronic Waste
This symbol (the crossed-out wheelie bin) means, that
this product should be brought to the return systems
and/or separate systems available to end-users according
to yours country regulations, when this product has
reached the end of its lifetime!
For details, please contact your local distributor!
This symbol applies only to the countries within the EEA*.
*EEA = European Economics Area, comprising all EUmembers plus Norway, Iceland and Liechtenstein.
22
11.0 Notes
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23
12.0 EU - Konformitätserklärung
EC - Declaration of Conformity
Göttingen, 2010
im Sinne der EG-Richtlinie über elektrische Betriebsmittel zur Verwendung
innerhalb bestimmter Spannungsgrenzen 73/23/EWG
following the EC directive about electrical equipment for use within certain
limits of voltage 73/23/EEC
und / and
im Sinne der EG-Richtlinie für die elektromagnetische Verträglichkeit
89/336/EWG.
following the EC directive about the electromagnetic compatibility
89/336/EEC .
Hiermit erklären wir, daß das folgende Power Pack,
Herewith we declare that the following Power Pack,
Typ / type:
Best.-Nr. / Order No.
Standard Power Pack P25 (Rev. b)
040-800
den grundlegenden Anforderungen der
corresponds to the basic requirements of
EG-Niederspannungsrichtlinie 73/23/EWG und der
EC low voltage directive 73/23/EEC and the
EG-Richtlinie über die elektromagnetische Verträglichkeit 89/336/EWG
entsprechen.
EC directive about the electromagnetic compatibility 89/336/EEC .
Folgende harmonisierte Normen wurden angewandt:
The following harmonized standards have been used:
EN 60601-1-2:2001
EN 55011:1998 + A1:1999 + A2:2002
EN 61000-3-3:1995 + A1:2001
EN 61010-1:2001
Dr. Jürgen Otte
Quality Manager
24
EN 61000-3-2:2000
EN 61000-6-1:2001
13.0 Warranty
This laboratory instrument is produced with the highest
practical standards of materials, workmanship, and design. The design and manufacture of parts have been
conceived with one purpose - to produce units which will
give satisfactory service.
Biometra GmbH guarantees this unit to be free from defects in materials or workmanship under normal use or
service for 24 month from date of shipment.
If, during this time, this unit proves defective in materials
or workmanship, Biometra GmbH will repair or replace it
free of charge if returned to us prepaid.
This guarantee does not cover damage in transit, damage caused by carelessness, misuse or neglect, or unsatisfactory performance as a result of conditions beyond
our control; or consequential losses as a result of failure
of our product.
25
Biometra GmbH
Rudolf-Wissell-Str. 30
D-37079 Goettingen
Phone: +49 (0)5 51 50 68 6-0
Fax: +49 (0)5 51 50 68 6-66
e-mail: [email protected]
internet: http://www.biometra.de
Service Department
Rudolf-Wissell-Str. 14-16
D-37079 Goettingen
Phone: +49 (0)5 51 50 68 6-10 or -12
Fax:
+49 (0)5 51 50 68 6-11
e-mail: [email protected]
26
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