User's Manual
YSI 1500 Sport
L-Lactate Analyzer
www.YSI.com
Table of Contents
1.
Safety.................................................................................................... 1-1
1.1
2.
Explanation of Symbols............................................................................................... 1-1
Introduction .......................................................................................... 2-1
2.1
Features........................................................................................................................ 2-1
2.2
Specifications............................................................................................................... 2-2
3.
Instrument Setup .................................................................................. 3-1
3.1
Unpacking.................................................................................................................... 3-1
3.2
Component Identification ............................................................................................ 3-2
3.3
Reagent Preparation..................................................................................................... 3-4
Automatic Mode .............................................................................................................. 3-4
Manual Mode.................................................................................................................. 3-5
3.4 Membrane Installation ................................................................................................. 3-6
3.5
Power ........................................................................................................................... 3-6
3.6
System Setup ............................................................................................................... 3-7
Date.................................................................................................................................. 3-9
Time............................................................................................................................... 3-10
RS232 Communication.................................................................................................. 3-11
Setting the LCD Contrast............................................................................................... 3-12
Displaying Numerical Values in RADIX ...................................................................... 3-13
Setting the Stirring Speed .............................................................................................. 3-13
Selecting the Displayed Units........................................................................................ 3-14
Selecting the Operational Mode .................................................................................... 3-15
Resetting System Default Parameters............................................................................ 3-16
3.7 Preparing the Fluid System........................................................................................ 3-18
3.8
4.
Printer Option ............................................................................................................ 3-19
Sample Collection, Preparation and Measurement............................ 4-1
4.1
5.
Sample Preparation...................................................................................................... 4-1
YSI Model 1501, 25 microliter Syringepet ..................................................................... 4-2
YSI Model 1502 Capillary Tube Injector ........................................................................ 4-3
Principles of Operation ........................................................................ 5-1
5.1
Sensor Technology ...................................................................................................... 5-1
5.2
Fluid System ................................................................................................................ 5-3
5.3
Measurement Methodology ......................................................................................... 5-4
5.4
Baseline Stability ......................................................................................................... 5-6
i
5.5
Calibration.................................................................................................................... 5-7
5.6
Linearity....................................................................................................................... 5-8
5.7
Temperature Compensation ......................................................................................... 5-9
5.8
Software Structure ....................................................................................................... 5-9
Microprocessor Self Test ................................................................................................. 5-9
Electrode Checks ............................................................................................................. 5-9
Temperature Error Checks ............................................................................................. 5-10
Battery Level Checks ..................................................................................................... 5-10
1500 SPORT Menu Flow Chart..................................................................................... 5-11
6.
Instrument Operation .......................................................................... 6-1
6.1
Calibration Cycle ......................................................................................................... 6-1
Calibration-Automatic Mode ........................................................................................... 6-1
Calibration-Manual Mode................................................................................................ 6-5
6.2 Sample Cycle ............................................................................................................... 6-8
6.3
Recalling Stored Results ............................................................................................ 6-10
Recalling All Sample Results ........................................................................................ 6-10
Recalling Today's Sample Results ................................................................................. 6-11
Recalling a Result From Any Previous Date ................................................................. 6-12
Recalling a Result By ID Number ................................................................................. 6-14
6.4 Printing Results.......................................................................................................... 6-15
Printing All Sample Results........................................................................................... 6-15
Printing Today's Results ................................................................................................ 6-16
Printing Previous Days' Results..................................................................................... 6-17
Printing Results By ID Number..................................................................................... 6-17
6.5 Transmitting Results Over the RS232 Communication Link.................................... 6-18
6.6
Deleting Stored Results.............................................................................................. 6-19
Editing Results ............................................................................................................... 6-19
Deleting All Results....................................................................................................... 6-20
6.7 Daily Operational Checks .......................................................................................... 6-21
7.
ii
Instrument Diagnostics ........................................................................ 7-1
7.1
Checking Probe Current............................................................................................... 7-1
7.2
Checking Sample Chamber Temperature .................................................................... 7-2
7.3
Checking the Sample Chamber Injection Switch Status.............................................. 7-3
7.4
Turning the Stir Motor On and Off.............................................................................. 7-3
7.5
Turning Pump Motors On and Off............................................................................... 7-4
7.6
Sending a Test Print String Over the Infrared Data Link ............................................ 7-5
7.7
Sending a Test Data String Over the RS232 Data Link............................................... 7-5
7.8
Testing the Keypad ...................................................................................................... 7-6
7.9
Testing the Display ...................................................................................................... 7-7
7.10
Checking the Software Version Number ................................................................. 7-8
7.11
Checking the Battery Voltage.................................................................................. 7-8
8.
Maintenance......................................................................................... 8-1
8.1
Daily Maintenance....................................................................................................... 8-1
Empty the Waste Bottles ................................................................................................. 8-1
Check Buffer and Reference Bottle Levels ..................................................................... 8-1
Check for Air Bubbles in Chamber ................................................................................. 8-1
Check for Leaks ............................................................................................................... 8-2
Clean up spills.................................................................................................................. 8-2
8.2 Preventative Maintenance............................................................................................ 8-2
8.3
Cleaning the Sample Chamber .................................................................................... 8-2
8.4
Membrane Replacement .............................................................................................. 8-3
8.5
Probe Cleaning............................................................................................................. 8-3
8.6
Tubing Replacement .................................................................................................... 8-4
8.7
Injection Port Seal Replacement.................................................................................. 8-6
8.8
Recharging the Battery ................................................................................................ 8-6
8.9
Storing the SPORT ...................................................................................................... 8-6
9.
Troubleshooting ................................................................................... 9-1
9.1
Fault Messages............................................................................................................. 9-2
Data System Faults .......................................................................................................... 9-2
Battery Faults................................................................................................................... 9-4
Enzyme Sensor Faults...................................................................................................... 9-4
Temperature Faults .......................................................................................................... 9-8
Fluid System Faults ......................................................................................................... 9-9
9.2 Possible Failure Conditions ....................................................................................... 9-10
10.
Appendix A–Typical Performance Data ............................................. 10-1
Capillary Tube Injector, YSI Model 1502 ..................................................................... 10-1
Blunt Needle Syringepet, YSI Model 1501 ................................................................... 10-3
11.
Appendix B–Sample Collection and Handling ................................... 11-1
Blood Plasma ................................................................................................................. 11-1
Blood Serum .................................................................................................................. 11-2
Whole Blood.................................................................................................................. 11-3
12.
Appendix C–Cell Lysing ...................................................................... 12-1
Using the YSI 1515 Cell Lysing Agent......................................................................... 12-2
Buffer Solution .............................................................................................................. 12-2
Reference Solution......................................................................................................... 12-2
13.
Appendix D Sample Preservation ...................................................... 13-1
14.
Appendix E–Effects Of Selected Substances ....................................... 14-1
iii
Reducing Agents............................................................................................................ 14-1
Calibration Shift by Detergents ..................................................................................... 14-2
Endogenous and Exogenous Substances ....................................................................... 14-3
Physical Damage............................................................................................................ 14-3
15.
Appendix F–Temperature Effect on Operation .................................. 15-1
Temperature Effect On Battery Life .............................................................................. 15-1
16.
Appendix G–Accessory Products ........................................................ 16-1
17.
Appendix H–Cleaning, Disinfecting and Decontamination ............... 17-1
18.
Appendix I–Warranty and Shipping Information ............................. 18-1
Cleaning Instructions ..................................................................................................... 18-2
Packing Instructions....................................................................................................... 18-3
19.
Appendix J–Required Notice.............................................................. 19-1
iv
1. Safety
1.1 Explanation of Symbols
WARNING
CAUTION
Warning indicates that misuse of the instrument could result
in death or serious injury to a person.
Caution, consult accompanying documents. Caution
indicates that misuse of the instrument could result in mild
or serious injury to a person and/or damage to equipment.
Biological Risks
Manufacturer
Authorized Representative in the European Union
In Vitro Diagnostic Medical Device
REF
2747
Catalog number
03A6549
Lot number
YEAR-MO
Date of manufacture
YEAR-MO
Use by Date
Temperature Limitation
1 -1
1 -2
2. Introduction
The YSI 1500 SPORT is a portable, battery powered blood lactate analyzer
intended for professional use in sports medicine applications. The SPORT is not
intended for home use. It is designed to provide quick measurement of lactate in
whole blood or plasma. Samples are injected with either a YSI 1501 blunt
needle syringepet or a YSI 1502 capillary tube holder/injector. The SPORT
automatically senses either of these injection devices, and from that point it
completes the sample analysis, displaying the result and readying itself for any
subsequent samples. Each sample is stored in memory, identified by date and
time, and also by athlete/sample ID number, if desired. You can recall and
display up to 150 sample values or you can transmit all of the stored data to a
personal computer via the RS232 communication port. An optional batterypowered printer is also available to make hard copy records of the results.
2.1
Features
•
Size: 13.4(W) x 14.8(L) x 7.0(H) inches, 10 lb.
33.0 x 27.9 x 17.8 cm, 4.53 kg.
•
Water resistant (splash-proof).
•
Rechargeable 12 VDC battery w/AC adapter. Minimum of 8 hours of use
between charges. See Appendix F–Temperature Effect on Operation.
•
Microprocessor control.
•
Alpha-numeric liquid crystal display, 16 character by 2 line with adjustable
contrast.
•
Menu-driven interface.
•
Automatic recognition of each sample injection.
•
Automatic calibration.
•
Athlete/sample ID# entry.
•
Sample result recall (up to 150 stored in memory).
•
Complete diagnostic software.
•
Temperature compensation over a limited range.
•
Printer option (infrared data link).
•
RS-232 capability to log data to a computer.
2 -1
2.2
Specifications
Sample size:
25 microliters
Sample Medium: Whole Blood; Plasma
Sampling Speed: Manual Mode:
Auto Mode:
60 seconds
96 seconds
Detection range:
0 to 30 mmol/L (0 to 270 mg/dL)
Resolution:
0.01 mmol/L (0.2 mg/dL)
Calibration point: 5 mmol/L (45 mg/dL)
Typical performance: See Appendix A for typical performance data for whole
blood and plasma.
Operating Temperature Range: 5 to 45°C, 10 to 90% RH, non condensing
Storage Temperature Range: 0 to 60°C, 10 to 90% RH, non condensing
Rechargeable at 95 to 130 VAC with the 115 volt charger, or at 190 to 240 VAC
with the 230 volt charger; 47 to 63 Hz.
Battery Charger Output: 13.8 VDC @ 300 milliamps
2 -2
3. Instrument Setup
We urge you to read through the entire Operations Manual before starting to use
the SPORT. Though the SPORT was designed for ease of operation, prompting
you through each operational step, it is still helpful to know as much as possible
about the instrument in order to get the most out of it.
3.1 Unpacking
Remove the instrument from its shipping container. Be careful not to discard
any parts or supplies. Check off all items on the packing list and inspect all
assemblies and components for damage. If any parts are damaged or missing,
contact YSI Customer Service or your Dealer Representative immediately.
Do not discard the shipping container.
If servicing is required, the instrument must be returned to the YSI Repair
Center. Field service is not available on this instrument. We strongly recommend
that you use the original shipping container for this purpose as it was designed
to protect the instrument during transport.
Reagents and injection devices for the SPORT analyzer are not packaged in the
same carton as the instrument. Reagents come in a starter kit (YSI 1504); and
injection devices (YSI 1501 or 1502) must be ordered separately.
WARNING: Always make sure the SPORT power is turned
off prior to connecting the battery charger. Failure to do
this could result in stored data corruption if the battery
has been discharged to a very low level.
3 -1
3.2 Component Identification
This section names the principal parts and describes their use. Figure 3.1 and
Figure 3.2 identify each part.
Figure 3 . 1 SPORT
3 -2
The Buffer, Reference and Waste Bottles in the rear of the instrument are used
to hold the solutions needed for sample analysis.
The Buffer Pump is a two-channel peristaltic pump driven by a gearmotor. One
channel, (the lower body), draws buffer from the Buffer Bottle and pumps it
through the Sample Chamber. The second channel, (the upper body), draws fluid
from the waste chamber and pumps it into the Waste Bottle.
The Reference Pump is identical in operation to the Buffer Pump. It is used to
pump the reference solution.
The Sample Chamber is made of clear acrylic plastic. White and black probe
holders are attached to the sides. The enzyme probe is in the white holder. The
membrane is mounted on an O-ring that serves as a fluid seal between the
chamber and the probe. The temperature probe is in the black holder. A small
black O-ring on the tip of the temperature probe serves as a fluid seal.
The Injection Port is on the top of the Sample Chamber. Inside the port is a
sensor that detects the insertion of a YSI injection device. A removable cap is
threaded into the top of the Injection Port. This cap must be removed and
replaced with the supplied threaded plug before moving or storing the
instrument.
The Keypad is a 15 key membrane switch with numeric keys from 0 to 9, and 5
function keys, ENTER, MENU, CANCEL, DELETE, and ON.
The Display is a 2 line by 16 character liquid crystal display.
Figure 3 . 2 SPORT Interface Panel
The following switches and connectors are located in a recessed area on the side
of the instrument.
The Reset Switch is used for a hard reset of the operating system.
3 -3
The Remote Communication Port is a 25 pin RS232C serial port. It is used to
interface with personal computers.
The Power Switch is a rocker switch that allows you to control power to the
instrument.
The Infrared Printer Port is used to transmit data to the optional battery
powered printer.
The Power Receptacle is a DC power inlet. The battery charger supplied with
the SPORT is connected here to recharge the instrument battery.
IMPORTANT: Probe polarization will be disrupted when power is turned off.
Time for restabilization will be needed when power is restored.
3.3 Reagent Preparation
If you are going to use only the manual mode of operation, the only solution you
will need to prepare is the Buffer solution as the Reference solution is only used
as part of the Automatic mode.
You can prepare the reagent solutions for the SPORT yourself. These reagents,
along with a mixing bottle and a graduated syringe (3cc), are provided in the
YSI Model 1504 Starter Kit which must be ordered separately. Please note that
the reagents in the starter kit may also be ordered separately.
IMPORTANT: The mixing bottle and Syringe must be cleaned every week. See
Section 8.2 Preventative Maintenance.
If your sample measuring protocol requires the total lysis of blood cells, or if
you are going to preserve the samples in YSI 2372 Preservative Tubes for later
analysis, you must refer to Appendix D Sample Preservation, for further
information before proceeding.
Automatic Mode
Buffer Solution
Fill the 500mL mixing bottle with reagent water. Reagent water must be very
pure. Use high quality deionized or distilled water. Add one package of YSI
2357 Buffer Concentrate and stir, waiting long enough to be certain that the
buffer chemicals have completely dissolved.
Pour buffer solution into the instrument Buffer Bottle. Put the cap back on the
Buffer bottle and place it back into the instrument.
3 -4
Reference Solution
Prepare 500 mL of Buffer solution, pour it into the Reference Bottle and add to
it 4.5 cc of YSI 1530 30 mmol/L Calibration Standard. This should be measured
out with the graduated syringe supplied in the 1504 Starter Kit. Replace the
bottle lid and shake the bottle for 15 seconds to insure complete mixing.. It is
important for reliable results that the reference solution is completely
mixed.
Rinse the mixing bottle with deionized or distilled water.
IMPORTANT: Screw the bottle lids on tightly before putting them back into the
instrument. If you don't, fluid may leak out when you pick up the SPORT by its
handle.
Manual Mode
Buffer Solution
Fill the 500mL mixing bottle with reagent water. Reagent water must be very
pure. Use high quality deionized or distilled water. Add one package of YSI
2357 Buffer Concentrate and stir, waiting long enough to be certain that the
buffer chemicals have completely dissolved.
Pour the buffer solution into the instrument Buffer Bottle. The remaining fluid
may be saved inside the mixing bottle until it is needed later.
Reagent Preparation
500 mL Reagent Water
One packet YSI 2357 Buffer Concentrate
4.5 cc YSI 1530 Lactate Standard
One packet YSI 1515 Cell Lysing Agent
500 mL Reagent Water
One packet YSI 2357 Buffer Concentrate
One packet YSI 1515 Cell Lysing Agent
Figure 3 . 3 Reagent Preparation
3 -5
3.4 Membrane Installation
The starter kit contains a pack of four YSI 2329 lactate membranes.
Figure 3 . 4 Enzyme Membrane Installation
To install a membrane, first unscrew the probe retainer and pull out the probe.
Remove the membrane assembly that's on it. You can use a toothpick or pointed
tool to unseat the old membrane. Be careful not to scratch the probe face. Open
one compartment of the plastic membrane pack and rinse the membrane inside
with a few drops of salt solution (YSI 2392 is provided in the 1504 starter kit).
Place one drop of salt solution on the probe face. Then, using the plastic
membrane pack as an applicator, press the O-ring membrane assembly gently
onto the probe face (Figure 3.4). Be sure the membrane is seated on the probe
face. The top of the O-ring will be flush with the shoulder. Wipe excess salt
solution from the probe body, then return the probe to the sample chamber.
Finger tighten the probe retainer so that the O-ring makes the necessary seal.
Return the membrane holder to the foil pouch and refrigerate it. Note the
expiration date on the membrane package. We recommend that you keep a
logbook in which the dates and lot numbers of reagents are recorded, along with
data from daily operational checks and other relevant information.
3.5 Power
If the SPORT is being powered up for the first time or it has been unused for
some period of time, the first thing you should do is connect the battery charger.
CAUTION: Make sure the SPORT power is turned off
before plugging in the battery charger.
3 -6
In normal use, you can leave the power on as long as you like provided the
battery charger is connected. Leaving the charger connected and the power on
will prevent the battery from discharging and requiring charging prior to the
next usage. If the SPORT is stored with the power on and the charger
disconnected, the battery will discharge to a level requiring a recharge within 3
days.
YSI recommends that the SPORT be used with the battery
charger whenever AC power is available.
IMPORTANT: Probe polarization will be disrupted when power is turned off.
Time for restabilization will be needed when power is restored.
Either of the following displays confirm correct power-up:
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
CONNECT CHARGER
PRESS ON
If the display prompts you to connect the charger, you may do so at this time
without turning off the power switch. The software will prohibit running until
the battery has been sufficiently recharged. Refer to Section 9 Troubleshooting
for more information.
If neither of these displays become active, press the Reset pushbutton located on
the side of the unit. If the SPORT fails to respond, turn off all power and call
YSI Technical Support.
3.6 System Setup
You use menus to set up the SPORT system. Once set up, the system parameters
are maintained in memory until they are changed purposely.
IMPORTANT:
IMPORTANT The SPORT allows you to go through each menu step by step by
selecting options on the display. Each menu has a number of options, and each
option is identified by a number. Pressing the number that corresponds to the
desired option moves the display cursor to that option. However, the selection
will not be made until you press ENTER. Whenever a new menu is displayed,
the cursor defaults to the option identified by the digit 0. Refer to Figure 3.5 for
the keypad layout and cursor location.
3 -7
Menu
Cancel
Numeric Keypad
Delete
On
Enter
Figure 3 . 5 SPORT Keypad
How the five function keys and the numeric keypad are used:
•
ONON ON is used as a user acknowledgement of a low battery condition.
•
ENTER - ENTER commits the data you've keyed in with the numeric
keypad. It does this from any menu at any level.
•
MENU - Each time you press MENU, you back up one level to the previous
menu.
•
CANCELCANCEL CANCEL stops the current operation, starts a wash cycle and
brings up the Main menu.
•
DELETE - DELETE is active only when the display is showing recalled
sample values. It is used to delete the value showing at the moment.
•
NUMERIC KEYPAD - The keys numbered from 0 to 9 allow you to enter
time, date, ID numbers, menu selections and other data.
The following sections in this chapter show you how to set up the instrument.
The correct display for each step is shown. If the display does not respond as
shown, press MENU and try again. Instructions are given starting each time
from the Main menu. As you become more familiar with the menu structure this
will not be necessary.
3 -8
Date
Go to Main menu by pressing M ENU until you see:
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Setup by pressing 2 , then ENTER.
ENTER
DATEDATE - 0 TIMETIME - 1
RS232RS232 - 2 MOREMORE - 3
Select Date setup by pressing ENTER.
ENTER
DATE MM/DD/YY
XX/XX/XX DAY
The first and second digits you press define the desired month; 01 is January and
12 is December. The third and fourth digits you press define the date; you can
enter any pair of digits from 01 to 31. The fifth and sixth digits define the last
two digits of the desired year. When you see the date you want on the screen,
press ENTER.
ENTER
Example: January 15, 2003 is entered as 01 15 03 ENTER
DATE MM/DD/YY
01/15/03 WED
If you have entered an impossible date, January 99, 2003 for example, the
display will read:
DATE? PRESS ENTER
01/99/03
Press ENTER to get back to the previous menu for another attempt to set the
date.
If the date you have entered is acceptable, press MENU until you return to the
Main menu.
3 -9
Time
The SPORT incorporates a 24 hour clock.
Go to Main menu by pressing MENU until you see:
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Setup by pressing 2 , then ENTER.
ENTER
DATEDATE - 0 TIMETIME - 1
RS232RS232 - 2 MOREMORE - 3
Select Time by pressing 1 , then ENTER.
ENTER
TIME HH:MM:SS
XX:XX:XX
The first and second digits that you press define the desired hour; 06 is 6 am and
18 is 6 pm. The third and fourth digits you press define the minute; you can use
anything between 00 and 59. The fifth and sixth digits define the seconds. If the
time you see on the display is what you want, press ENTER.
ENTER
Example: Nine thirty in the morning would be entered as 09 30 00 ENTER
TIME HH:MM:SS
09:30:00
If you have entered an impossible time, 99:00:00 for example, the display will
read...
TIME? PRESS ENTER
99:00:00
Press ENTER to return to the previous menu for another attempt to enter the
time.
If the time of day you have entered is accepted, press MENU until you return to
the Main menu.
3-10
RS232 Communication
It is possible to send all of the stored sample data to a personal computer, but in
order to do so a communication software package is required to be installed in
the PC. Once the communication package is installed in the PC, it is simply a
matter of making sure the settings of the PC and the SPORT are the same. The
SPORT is flexible and allows many choices.
Go to Main menu by pressing MENU until you see:
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Setup by pressing 2 , then ENTER.
ENTER
DATEDATE - 0 TIMETIME - 1
RS232RS232 - 2 MOREMORE - 3
Select RS232 by pressing 2 , then ENTER.
ENTER
DATADATA- 0 BAUDBAUD- 1
STOPSTOP - 2 PARITYPARITY - 3
Select bit data by pressing ENTER.
ENTER
7 BIT
BIT-- 0
8 BITBIT - 1
Select the desired setting by number, then ENTER.
ENTER The display will return to
the previous menu.
DATADATA - 0 BAUDBAUD - 1
STOPSTOP - 2 PARITYPARITY - 3
Select baud rate by pressing 1 , then ENTER.
ENTER
300 BAUDBAUD - 0
1200 BAUDBAUD - 1
Select the desired setting by number, then ENTER.
ENTER The display will return to
the previous menu.
DATADATA - 0 BAUDBAUD - 1
STOPSTOP - 2 PARITYPARITY - 3
3-11
Select stop bits by pressing 2 , then ENTER.
ENTER
STOP BITS
1 - 0 22-1
Select the desired setting by number, then ENTER.
ENTER The display will return to
the previous menu.
DATADATA - 0 BAUDBAUD - 1
STOPSTOP - 2 PARITYPARITY - 3
Select parity by pressing 3 , then ENTER.
ENTER
NONENONE - 0 EVENEVEN - 1
ODDODD - 2 HIHI - 3 LOLO - 4
Select the desired setting by number, then ENTER.
ENTER The display will return to
the previous menu.
Setting the LCD Contrast
Go to Main menu by pressing MENU until you see:
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Setup by pressing 2 , then ENTER.
ENTER
DATETIME
IMEDATE - 0 T
IME - 1
RS232RS232 - 2 MOREMORE - 3
Select More by pressing 3 , then ENTER.
ENTER
LCDLCD - 0 RADIXRADIX - 1
STIRSTIR - 2 MOREMORE - 3
Select LCD by pressing ENTER.
ENTER
LCD
DARKERDARKER - 0
LIGHTERLIGHTER - 1
The contrast can be adjusted up and down by pressing the appropriate number
3-12
and then the ENTER key. The adjustment will increment with each pressing of
the ENTER key.
Press MENU until the Main menu reappears.
Displaying Numerical Values in RADIX
The default setting shows a period separating the integer part of the number
from the decimal fraction. If you turn on the RADIX mode, a comma will
appear instead of a period.
Go to Main menu by pressing MENU until you see:
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Setup by pressing 2 , then ENTER.
ENTER
DATEDATE - 0 TIMETIME - 1
RS232RS232 - 2 MOREMORE - 3
Select MORE by pressing 3 , then ENTER.
ENTER
LCDLCD - 0 RADIXRADIX - 1
STIRSTIR - 2 MOREMORE - 3
Select the RADIX mode by pressing 1 , then ENTER.
ENTER
RADIX
PERIODPERIOD - 0 COMMACOMMA - 1
Turn the RADIX mode on by pressing 1 , then ENTER.
ENTER
Press MENU until the Main menu reappears.
Setting the Stirring Speed
The stirring speed can be adjusted to assure consistent stirring. When making
this adjustment, speed up the stirring until the stir bar tumbles inside the sample
chamber. Once you have done this slowly reduce the stirring speed until the stir
bar settles into a uniform spinning motion.
Go to Main menu by pressing MENU until you see:
3-13
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP- 2 DIAGDIAG- 3
Select Setup by pressing 2 , then ENTER.
ENTER
DATEDATE- 0 TIMETIME- 1
RS232RS232 - 2 MOREMORE - 3
Select More by pressing 3 , then ENTER.
ENTER
LCDLCD - 0 RADIXRADIX - 1
STIRSTIR - 2 MOREMORE - 3
Select Stir by pressing 2 , then ENTER.
ENTER
STIR SPEED
UPUP - 0 DNDN - 1
Pressing the ENTER key will speed up the stirring by a preset increment.
Pressing 1 , then ENTER will slow down the stirring by a preset increment.
Press MENU until the Main menu reappears.
Selecting the Displayed Units
Go to Main menu by pressing M ENU until you see:
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Setup by pressing 2 , then ENTER.
ENTER
DATEDATE - 0 TIMETIME - 1
RS232RS232 - 2 MOREMORE - 3
Select More by pressing 3 , then ENTER.
ENTER
LCDLCD - 0 RADIXRADIX - 1
STIRSTIR - 2 MOREMORE - 3
3-14
Select More by pressing 3 , then ENTER.
ENTER
UNITSUNITS - 0 MODEMODE - 1
SET DEFAULTDEFAULT - 2
Select Units by pressing ENTER.
ENTER
UNITS: mg/dL
mg/dL-- 0
mmol/Lmmol/L - 1
Select mg/dL by pressing ENTER,
ENTER or select mmol/L by pressing 1 , then
ENTER.
ENTER
Press MENU until the Main menu appears.
Selecting the Operational Mode
Go to Main menu by pressing MENU until you see:
RUNRUN- 0 RECALLRECALL- 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Setup by pressing 2 , then ENTER.
ENTER
DATEDATE - 0 TIMETIME - 1
RS232RS232- 2 MOREMORE- 3
Select More by pressing 3 , then ENTER.
ENTER
LCDLCD - 0 RADIXRADIX - 1
STIRSTIR - 2 MOREMORE - 3
Select More by pressing 3 , then ENTER.
ENTER
UNITSUNITS - 0 MODEMODE - 1
SET DEFAULTDEFAULT - 2
Select Mode by pressing 1 , then ENTER.
ENTER
3-15
SET CAL MODE AUTOAUTO - 0
MANUALMANUAL- 1
Select Auto mode by pressing ENTER,
ENTER or select Manual mode by pressing 1 ,
then ENTER.
ENTER
IMPORTANT:
IMPORTANT After pressing 1 then ENTER to select Manual mode, the cursor
returns to its default location (under the 0), however, the instrument is now in
Manual mode.
Press MENU until the Main menu appears.
Resetting System Default Parameters
If the system is reset, all stored results will be erased;
time, date and stir speed will have to be manually reset to
correct settings; and the analyzer will require
recalibration.
The system will be reset to a series of default settings which are listed below.
RS232 Communications:
Baud rate:
Data:
Stop bits:
Parity:
1200
8 bits
1
None
Mode:
Stir speed:
Automatic Calibration
Maximum
Memory:
Cleared: All stored results deleted
Radix:
Period
Units:
mg/dL
Go to Main menu by pressing MENU until you see:
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Setup by pressing 2 , then ENTER.
ENTER
3-16
DATEDATE - 0 TIMETIME - 1
RS232RS232- 2 MOREMORE- 3
Select More by pressing 3 , then ENTER.
ENTER
LCDLCD- 0 RADIXRADIX- 1
STIRSTIR - 2 MOREMORE - 3
Select More by pressing 3 , then ENTER.
ENTER
UNITSUNITS - 0 MODEMODE - 1
SET DEFAULTDEFAULT - 2
Select Default by pressing 2 , then ENTER.
ENTER
ARE YOU SURE?
NO - 0 YES - 1
To set all system parameters to the default settings press 1 , then ENTER.
ENTER The
display will read
A FEW SECONDS
DELAY...
UNITSUNITS - 0 MODEMODE - 1
SET DEFAULTDEFAULT - 2
Press MENU to return to the Main menu.
3-17
3.7 Preparing the Fluid System
Once you have setup the electronics of the instrument, filled both supply bottles,
and emptied the waste bottle, you are ready to prime the fluid system. You must
return to the Main menu.
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Press ENTER to get to the Run mode. You will see this display:
SAMPLESAMPLE - 0
CALCAL - 1 PRIMEPRIME - 2
Select PRIME by pressing 2 , then ENTER.
ENTER You will see this display:
PRIMING...
REF ON STIR ON
The instrument will run the reference fluid pump for approximately 30 seconds
in order to fill the tubing lines. Once the cycle has ended you will see this
display:
NOTE: The reference pump will only cycle if the selected mode is Automatic, if
Manual has been selected only the wash cycle will be called.
WASHING...
The instrument will run the buffer fluid pump for approximately 30 seconds in
order to fill the tubing lines. Once the cycle has ended you will see:
PRESS MENU
Press MENU to return to the previous display, you are now ready to calibrate
and run samples on your instrument.
3-18
3.8 Printer Option
While there is not a printer built into the instrument, there is a optional printer
available either through YSI (Model 1506), or your local Hewlett Packard
dealer. This printer, HP Model 82240, is a battery-powered unit controlled by an
infrared signal that is generated from the SPORT. The signal originates from the
recessed interface panel located on the right hand side of the instrument. It is
important that the printer be positioned in such a way as to not interrupt this
signal. This is important because the SPORT sends data only and does not have
the ability to acknowledge the reception of the data. Please refer to Figure 3.6
Printer Placement for positioning details.
Figure 3 . 6 Printer Placement
The instrument has the capability to print the data while running the samples or
you may print the results later, selectively by date or sample identification
number. Refer to Section 6.3 Recalling Stored Results.
Printing the results as you run samples also allows you access to information
that is only available during this cycle. Along with the actual result you will also
have a record of the temperature, initial baseline current, sample current, and
reference current. The exact sample format is shown in Figure 3.7 Print Format.
3-19
A similar printout is generated when calibrating and checking linearity. We
recommend that the printer be used in this manner whenever possible for two
reasons. One, if there were a complete electrical failure or accidental reformatting of the memory, it could be possible that the sample results in
memory may be lost or irretrievable. Two, the additional information on the
printout is extremely helpful in detecting performance trends should the need
arise for troubleshooting.
SAMPLE
---------------------------------TEMP
22.44 C
BASCUR 1.28 nA
SAMCUR 21.73 nA
REFCUR1 11.12 nA
---------------------------------04/08/98
09:08
# 001
10.07 mmol/L
+++++++++++++++++++++
F igure 3 . 7 Print Format
The exact format for a calibration or linearity printout provides the same
relevant information but appears slightly different.
3-20
4. Sample Collection, Preparation and Measurement
The SPORT is a versatile instrument that allows you to manipulate the sample in
a number of different ways.
•
You can collect the blood sample using a lancette and capillary tube, or by
venapuncture into a tube or other collection device, such as a vacutainer.
•
The SPORT can be used with 2 injection devices. The YSI 1501 25
microliter syringepet, ideal for drawing samples from a blood collection
tube, and the YSI 1502 25 microliter capillary tube injector, designed for
injection directly from a YSI capillary tube.
•
The sample can be prepared for immediate assay, or for later analysis. If you
are going to preserve your sample for later analysis, there are specific
instructions that must be followed in order to insure the most accurate
results. Please refer to Appendix D Sample Preservation.
For more information on obtaining blood samples, we refer you to two
publications:
Approved Standard Procedures for the Collection of Diagnostic Blood
Specimens by Skin Puncture, edited by Jean M. Slockbower. National
Committee for Clinical Laboratory Standards, Volume 2 Number 5, pages 132149, 1982.
Collection and Handling of Laboratory Specimens. A Practical Guide, edited by
Jean Slockbower and Thomas A. Blumenfield. J. B. Lippincott Company,
Philadelphia, 1983. ISBN 0-397-50520-5.
4.1 Sample Preparation
The blood from a finger or earlobe prick should be collected in a capillary tube.
If you use one of the YSI tubes, which are preset to 25 microliters, you must
inject it with the capillary tube injector. You can also draw venapuncture blood
from a vial, vacutainer, or YSI Preservative Tube with the preset tube.
If the capillary tube has a capacity greater than 25 microliters, the blood sample
must be transferred to the YSI 1501 Syringepet for injection. You can also use
the Syringepet to sample and inject blood from a venapuncture vial or
vacutainer.
The following sections instruct you in the use of both injection devices.
4 -1
YSI Model 1501, 25 microliter Syringepet
The Syringepet can be used to extract a 25 microliter sample from either a
vacutainer or a capillary tube of greater volume than 25 microliters, see Figure
4.1.
Begin with a sample volume greater than 75 microliters. To remove any
contaminants from the Syringepet, you should rinse it three times with buffer or
reagent-quality water. For maximum accuracy you should rinse it with buffer or
reagent water at least twice, and then with the sample twice, before loading it
for injection.
WARNING: Precision and accuracy are dependent on sample loading
technique. Any air in the sample column will cause incorrect results.
Figure 4 . 1 1501 Syringepet
Using the Syringepet may feel awkward at first, but you must master the
technique to get accurate results. Practice injecting Calibration standards into
the instrument. This will allow you to develop the necessary technique while
also letting you visually assess how well you are doing by checking the result on
the display.
Depress the plunger all the way with your thumb. Lower the Syringepet needle
into the sample, then slowly,
slowly using light thumb pressure, let the plunger come
all the way back.
Examine the glass barrel to see if there are any air bubbles in the sample. If
there are any, dispense the sample back into the container you drew it from and
try again. If there are no air bubbles present you are ready to inject the sample
into the instrument.
Once you are sure the sample is ready for injection, hold the Syringepet directly
above the injection port and slide the needle in until you feel it stop. Press in the
plunger all the way with your thumb. Keeping thumb pressure on the plunger,
remove the Syringepet from the port.
4 -2
WARNING: Any sample carryover on the outside of the needle could affect the
measurement. You should wipe off the tube or Syringe pet needle with a sterile
wipe, like a Kimwipe, prior to each sample injection.
YSI Model 1502 Capillary Tube Injector
YSI 1505 capillary tubes are provided in the Starter Kit. These tubes, which can
be ordered separately, are preset to a volume of 25 microliters. There is a double
white band on the tube opposite the end that you put into the sample.
You may load the capillary tube into the injector before collecting the sample or
you may collect the sample first, then load the tube.
WARNING: Precision and accuracy depend on sample loading technique. Any
air in the sample column will cause incorrect results.
Plunger
Handle
Tube clamp
Tube Clamp Window
Black band
Double white band
YSI 1505 Capillary Tube
Figure 4 . 2 1502 Capillary Tube Injector
The end of the tube with the double white band goes into the injector first. The
collar around the front part of the injector barrel is the tube clamp. Each time
you insert or withdraw a capillary tube, you loosen or tighten the tube clamp so
that the capillary tube can be inserted or withdrawn easily without sticking. Just
a small adjustment of the tube clamp one way or the other is necessary.
Slide the tube all the way in until it stops. The tube should be sticking out about
1/2 an inch (12 mm), sample will be visible in the section of the tube that is
protruding, and the black band should be visible in the tube clamp window.
4 -3
When you adjust the clamp, it is not necessary to unscrew it completely. Never
should more than one full turn be necessary.
WARNING: Any sample carryover on the outside of the tube could affect the
measurement. You should wipe off the tube or Syringepet needle with a sterile
wipe, like a Kimwipe.
Hold the injector directly above the injection port, and slide it into the port until
you feel it stop. In one motion, press the plunger all the way down with your
thumb. Maintaining pressure on the plunger after it has bottomed out, remove
the injector from the port.
Release the tube clamp and slide the used tube out of the injector.
WARNING: The used capillary tube is a biohazard and should be disposed of in
a manner consistent with safety and the laws in your area.
4 -4
5. Principles of Operation
5.1 Sensor Technology
The sensor technology of the YSI SPORT is based on the principles conceived
by Dr. Leland Clark, formerly of Children's Hospital Foundation, Cincinnati,
Ohio. The immobilized enzyme membrane was invented by YSI and is covered
by U.S. Patent 4,073,713. This sensor technology has been used successfully
since 1975 in the YSI 23A Blood Glucose Analyzer; then later in the YSI 27
Industrial Analyzer, the YSI 23L Blood Lactate Analyzer, the YSI 2300 STAT
Glucose/L- Lactate Analyzer, and the YSI 2700 SELECT Chemistry Analyzer.
Figure 5 . 1 Sensor Probe and Enzyme Membrane
The probe is fitted with a three-layer membrane containing immobilized lactate
oxidase in the middle layer. Figure 5.1 shows an exploded view of the
membrane and its relationship to the face of the probe. The face of the probe,
covered by the membrane, is situated in a buffer-filled sample chamber into
which a sample is injected. Some of the substrate diffuses through the
membrane. When it contacts the immobilized enzyme (lactate oxidase), it is
rapidly oxidized, producing hydrogen peroxide (Reaction 1). The hydrogen
peroxide (H2O2) is, in turn, oxidized at the platinum anode, producing electrons
(Reaction 2). A dynamic equilibrium is achieved when the rate of H2O2
production and the rate at which H2O2 leaves the immobilized enzyme layer
become constant. Equilibrium is indicated by a steady state response. The
electron flow is linearly proportional to the steady state H2O2 concentration and,
therefore, to the concentration of lactate.
5 -1
Reaction 1 (lactate):
Lactate + O2
LOx
Pyruvate + H2O2
Reaction 2: H2O2
Pt anode
2H+ + O2+ 2e-
The platinum electrode is held at an anodic potential and is capable of oxidizing
many substances other than H2O2. To prevent these reducing agents from
contributing to sensor current, the membrane contains an inner layer consisting
of a very thin film of cellulose acetate. This film readily passes H2O2but
excludes chemical compounds with molecular weights above approximately
200. The cellulose acetate film also protects the platinum surface from proteins,
detergents and other substances that could foul it. However, the cellulose acetate
film can be penetrated by such compounds as hydrogen sulfide, low molecular
weight mercaptans, hydroxylamines, hydrazines, phenols and anilines.
Certain detergents, endogenous and exogenous substances, preservatives and
reducing agents can interfere with the SPORT sensor system, resulting in
erroneous readings. (See Appendix E–Effects Of Selected Substances).
5 -2
5.2 Fluid System
Figure 5 . 2 SPORT Fluid System
5 -3
5.3 Measurement Methodology
The algorithm used to calculate results allows the SPORT to process samples as
quickly as possible while maintaining high accuracy and reliability. The result is
calculated from a group of four readings, depending on the chosen operational
mode:
•
Initial baseline current
•
Sample / Calibrator plateau current
•
Reference plateau current (Auto mode only)
•
Calibrator concentration value current
For initial calibration, 25 microliters of calibration standard is dispensed into the
chamber, which already contains approximately 500 microliters of buffer. The
sensor response increases until it reaches a plateau, approximately 30 seconds.
The reference pump then flushes the chamber for approximately 33 seconds and
the sensor response changes to a different value. At this point the instrument
calculates the relationship of the known standard to the reference solution and
stores this information until another calibration injection is performed. The
Buffer Pump then flushes the chamber for approximately 33 seconds and the
sensor response decreases to the normal baseline value.
During each subsequent sample cycle, the reference solution is again measured
and then internally compared to the reference solution concentration measured
during the initial calibration. If a variation exists, the calculation of the sample
concentration will be compensated accordingly.
The profile of probe current response is shown in Figure 5.3 (Auto Mode) and
Figure 5.4 (Manual mode).
5 -4
Figure 5 . 3 Automatic Mode Probe Current Profile
5 -5
Figure 5 . 4 Manual Mode Probe Current Profile
5.4 Baseline Stability
The SPORT checks the probe baseline current for stability whenever it enters
the Sample mode. The baseline current must be within an acceptable range (less
than 6 nanoamps) in order for the instrument to perform to specification. If the
baseline current is greater than 6 nA an error message will be displayed, (see
Section 9 Troubleshooting.
5 -6
5.5 Calibration
The SPORT uses one lactate standard (YSI 2327 5 mmol/L) for calibration and
two lactate standards are available for checking linearity (YSI 2328 15mmol/L
and YSI 1530 30 mmol/L).
The following is the recommended calibration frequency:
Auto Mode
•
Every day at the beginning of testing
•
Whenever you change the membrane or install new reagent fluids
•
Whenever you turn the instrument power on
•
Every two hours while testing
•
Whenever you change injection devices
Manual Mode
•
Every day at the beginning of testing
•
Whenever you change the membrane or install new reagent fluids
•
Whenever you turn the instrument power on
•
For maximum accuracy after every fifth sample
•
Whenever you change injection devices
Figure 5 . 5 Calibration Algorithms
Calibrating the SPORT in Manual Mode with the 5 mmol/L standard basically
compares the relative concentration of the standard with the relative
concentration of the unknown sample. Calibrating in Automatic Mode compares
the relationships of the calibration standard to the reference solution with the
relationship of the unknown sample concentration to the reference solution, this
5 -7
relationship is then used in calculating the final concentration of the sample that
is being measured. Refer to Figure 5.5 for the algorithms used.
WARNING: Running samples with an injection device
other than the device used to calibrate the instrument may
result in erroneous data.
5.6 Linearity
The sensor consists of an electrode and an enzyme membrane. As the membrane
ages, it becomes underlinear, or less sensitive (see Figure 5.6).
Linear R esponse
M em brane
R esponse
Analyzer
R esponse
C alibration
Substrate C oncentration
Figure 5 . 6 Aging Membrane Response
You can check linearity with YSI 1530 30 mmol/L standard, after every
calibration. You can also check it at "mid-range" with YSI 2328 15 mmol/L
standard. Both standards should be injected as if it were a sample.
Both standards, however, can be measured as samples any time you want to
check the linearity manually.
Acceptable Linearity Ranges:
For YSI 2328 Lactate Standard: 14.30 to 15.70 mmol/L
(128.70 to 141.30 mg/dL)
For YSI 1530 Lactate Standard: 28.50 to 31.50 mmol/L
(256.50 to 283.50 mg/dL)
For the very highest accuracy, you should check linearity every hour of use.
5 -8
5.7 Temperature Compensation
The sensitivity of the sensor varies with temperature changes. The higher the
temperature, the more sensitive the sensor becomes. A temperature
compensating algorithm has been implemented to adjust for a maximum change
of 3 degree Celsius within each sample cycle in auto mode, (Sample temp. vs
Reference temp.) or from the most recent calibration in manual mode. If a
greater change than this is occurring, the rate of change is too fast to guarantee
reliable results. Error checking routines will display an error message if this is
the case.
It is reasonable to expect that using the SPORT outside may result in some
instances where the temperature rate of change (rising and falling) is quite fast.
Please refer to Appendix F–Temperature Effect on Operation for further
information about this subject.
5.8 Software Structure
The SPORT incorporates self-test and error detection capabilities to prevent
reporting erroneous results.
Microprocessor Self Test
If at initial power up the display does not become active, press the reset button.
If this does not correct the problem, try turning the main power switch off and
on again. If this does not help, call YSI Technical Support for repair assistance.
Also at power up and prior to each sample cycle, the processor does a fast self
test of the electronics to see if any stored data has been corrupted, or if the
software has been physically changed. If changes are detected, warning
messages will be displayed. Refer to Section 9 Troubleshooting for a listing of
the error messages and the possible causes and corrective actions.
Electrode Checks
The background current and noise levels of the electrode are monitored prior to
and during both the calibration and sampling cycles. If the error limits are
exceeded, an appropriate error message will be displayed. Refer to Section 9
Troubleshooting for a listing of the error messages and the possible causes and
corrective actions.
5 -9
Temperature Error Checks
The sample chamber temperature is checked at several different times during
sampling to assure that the rate of change is not adversely affecting result
reliability. If the error limits are exceeded, an appropriate error message will be
displayed. Refer to Section 9 Troubleshooting for a listing of the error messages
and the possible causes and corrective actions.
Battery
Battery Level Checks
The battery voltage is checked at several different times during sampling to alert
you to conditions that may require reconnecting the battery charger. The
software will not allow continuation below certain levels as the stored data can
be corrupted if the battery gets to very low levels. Warnings will be displayed at
12.1 volts and a software lockout will occur at 12.0 volts. Once the software
lockout is enabled, the battery charger must be connected. This lockout will
remain in effect until the battery is recharged to 12.8 volts. A separate electrical
hardware shut off will occur at 11.5 volts to prevent any accidental complete
discharging of the battery if the SPORT sits unattended for long periods of time.
For more information refer to Section 8.8 Recharging the Battery, and Section 9
Troubleshooting for a complete description of the warning messages and
corrective actions.
5-10
1500 SPORT Menu Flow Chart
Main Menu
RUN-0 RECALL-1
SETUP-2 DIAG-3
Run Mode
Sample
ID#?
Calibrate
Cal Check
Prime Pumps
Recall Mode
Display
View Results
Setup Mode
Date
Time
RS232
All
Date
ID#
Delete Results
All
Individually
Print Results
All
Date
ID#
Xmit Results
Diagnostic Mode
Probe Current
Run Ref. Cycle
Run Wash Cycle
Baud Rate
Chamber Temp.
Data Bits
Injection Switch
Stop Bits
Stir Motor
Parity
Pumps
LCD Contrast
Radix Mode
Stir Speed
Reference Pump
Buffer Pump
Print
Units/Measure
mg/dL
mmol/L
Operational Mode
Auto
Manual
System Default
Infrared Link
RS232 Link
Keypad
Display
Version #
Battery Voltage
Format Memory (erase)
Set RS232 Default
Start Micro. Clock
Stir Speed (Maximum)
mg/dL
Figure 5 . 7 Menu Flow Chart
5-11
5 -1 2
6. Instrument Operation
IMPORTANT:
IMPORTANT You should check the probe current before beginning tests. (See
section 7 Instrument Diagnostics. If there are any problems, you don't want to
discover them after you have begun.
Step by step instructions in this chapter will help you learn the operation of the
SPORT. Instructions for each step are followed by an illustration of the display
as it should respond. If the display does not respond correctly, press MENU and
try again. It is not uncommon for an instrument to be unstable for the first hour
or so once it has been initially setup and calibrated. This is a result of the
membrane requiring time to break in and also because the surface of the enzyme
probe requires time to condition itself with respect to the new membrane and
fluids. As a result of this YSI recommends that in the first hour of use after
initial setup the instrument should be checked several times for accuracy
(use YSI standards), and calibrated as often as required.
6.1 Calibration Cycle
There are two modes of operation allowed on the SPORT, Automatic and
Manual mode. Each have their own calibration process which are described in
the sections that follow.
CalibrationCalibration -Automatic Mode
In Automatic mode, you should calibrate the SPORT on these occasions:
•
Every day at the beginning of testing
•
Whenever you change the membrane or install new reagent fluids
•
Whenever you turn the instrument on (automatically prompted)
•
Whenever you change injection devices
It is recommended that a sample of the 5 mmol calibration standard should be
ran at least once every two hours while testing in order to check calibration
status. If the result is outside the specified range, then recalibration is necessary.
Prepare buffer and reference solutions and have the 5 mmol/L, 15 mmol/L and
30 mmol/L calibration standards provided in the 1504 Starter Kit at hand. With
the SPORT set up as described in Section 3.3, proceed as follows:
Load the Syringepet or injector with 5 mmol/L standard.
WARNING: If you calibrate with the Syringepet, measure with the Syringepet.
If you calibrate with the injector, measure with the injector. Otherwise, you
may get incorrect values.
6 -1
Press MENU until the following display appears.
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Run by pressing ENTER.
ENTER
SAMPLESAMPLE - 0
CALCAL - 1 PRIMEPRIME - 2
Select the Calibration cycle by pressing 1 , then ENTER.
ENTER
WAIT...
If the baseline probe current is not within the expected range the display will
read:
ERROR: BASELINE
PRESS MENU
If you see this display press MENU,
MENU then see Section 9 Troubleshooting.
If there is no problem, the display will read
INJECT 5mmol/L
STANDARD...
Inject the 5 mmol/L standard. When you remove the injection device, you
should see
RUNNING...
STIR ON
RUNNING...
REF ON
WASHING...
If the probe current is outside the expected limits, you will see
6 -2
ERROR: MEMB SENS
If you see this display press MENU,
MENU then see Section 9 Troubleshooting.
If there is no problem, the display will read
WAIT...
INJECT 5 mmol
CALIBRATOR CHECK
Inject the 5 mmol/L standard to verify a successful calibration. Now you should
see
RUNNING...
RUNNING ...
STIR ON
RUNNING...
REF ON
WASHING...
CAL CHECK
XX.XXmmol
The acceptable range is from 4.90 to 5.10 mmol/L (44.1 to 45.9 mg/dL). If the
result is outside of this range, repeat the calibration process in order to eliminate
the possibility of improper injection technique. If the calibration was successful
press MENU.
MENU
SAMPLESAMPLE - 0
CALCAL - 1 PRIMEPRIME - 2
Check the linearity of the membrane by injecting a sample of either the YSI
2328 15 mmol or the YSI 1530 30 mmol lactate standard.
6 -3
Press ENTER,
ENTER the display will read...
ENTER ID# XXX
No ID# is required so press ENTER,
ENTER the display will read...
WAIT...
INJECT SAMPLE...
Inject the sample. When you remove the Syringepet or Injector, you will see
RUNNING...
STIR ON
RUNNING...
REF ON
#XXX XX.XXmmol/L
WASHING...
MM/DD/YY HH:MM
#XXX XX.XXmmol/L
The sample result will be displayed along with the ID# (in this case 000), date
and time.
Linearity failure means that the instrument is not performing to specifications at
the upper limit of the dynamic range. This could be due to poor membrane
performance, check the date the membrane was installed to verify that it has not
expired, (also verify that the membrane has not been subjected to drying out as a
result of no fluid in the sample chamber). The acceptable ranges are:
15 mmol: 14.30 to 15.70 mmol (128.3 to 141.7 mg/dL)
30 mmol: 28.50 to 31.50 mmol (256.5 to 283.5 mg/dL)
6 -4
If the result is not within these ranges, replace the membrane, allow time for it
to break in and repeat the calibration process.
If the result is acceptable press MENU and the display will read
SAMPLESAMPLE - 0
CALCAL - 1 PRIMEPRIME - 2
You are now ready to measure samples.
CalibrationCalibration -Manual Mode
In Manual Mode, you should calibrate the SPORT on these occasions:
•
•
•
•
•
Every day at the beginning of testing
Whenever you change the membrane or install new reagent fluids
Whenever you turn the instrument on (automatically prompted)
After every fifth sample
Whenever you change injection devices
•
A 3°C temperature change since the last calibration (automatically prompted)
Prepare buffer and solution and have the 5 mmol/L, 15 mmol/L and 30 mmol/L
calibration standards provided in the 1504 Starter Kit at hand. With the SPORT
set up as described in Section 3.3, proceed as follows:
Load the Syringepet or injector with 5 mmol/L standard.
WARNING: If you calibrate with the Syringepet, measure with the Syringepet.
If you calibrate with the injector, measure with the injector. Otherwise, you may
get incorrect values.
Press MENU until the following display appears.
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Run by pressing ENTER.
SAMPLESAMPLE - 0
CALCAL - 1 PRIMEPRIME - 2
Select the Calibration cycle by pressing 1 , then ENTER.
ENTER
WAIT...
6 -5
If the baseline probe current is not within the expected range the display will
read:
ERROR:
ERROR : BASELINE
PRESS MENU
If you see this display press MENU,
MENU then see Section 9 Troubleshooting. If
there is no problem, the display will read:
INJECT 5mmol/L
STANDARD...
Inject the 5 mmol/L standard. When you remove the injection device, you
should see:
RUNNING...
STIR ON
WASHING...
If the probe current is outside the expected limits, you will see:
ERROR: MEMB SENS
If you see this display press MENU, then see Section 9 Troubleshooting.
If there is no problem, the display will read:
WAIT...
INJECT 5 mmol
CALIBRATOR CHECK
Inject the 5 mmol/L standard to verify a successful calibration.
Now you should see:
RUNNING...
STIR ON
6 -6
WASHING...
CAL CHECK
XX.XXmmol
The acceptable range is from 4.90 to 5.10 mmol/L (44.1 to 45.9 mg/dL). If the
result is outside of this range, repeat the calibration process in order to eliminate
the possibility of improper injection technique. If the calibration was successful,
press MENU.
MENU
SAMPLESAMPLE - 0
CALCAL - 1 PRIMEPRIME - 2
Check the linearity of the membrane by injecting a sample of either the YSI
2328 15 mmol or the YSI 1530 30 mmol lactate standard.
Press ENTER,
ENTER the display will read:
ENTER ID# XXX
No ID# is required so press ENTER,
ENTER the display will read:
WAIT...
INJECT SAMPLE...
Inject the sample. When you remove the Syringepet or Injector, you will see:
RUNNING...
STIR ON
#XXX XX.XXmmol/L
WASHING...
6 -7
MM/DD/YY HH:MM
HH:M M
#XXX XX.XXmmol/L
The sample result will be displayed along with the ID#, date and time.
Linearity failure means that the instrument is not performing to specifications at
the upper limit of the dynamic range. This could be due to poor membrane
performance, check the date the membrane was installed to verify that it has not
expired, (also verify that the membrane has not been subjected to drying out as a
result of no fluid in the sample chamber). The acceptable ranges are:
15 mmol: 14.30 to 15.70 mmol (128.3 to 141.7 mg/dL)
30 mmol: 28.50 to 31.50 mmol (256.5 to 283.5 mg/dL)
If the result is not within these ranges, replace the membrane, allow time for it
to break in and repeat the calibration process. If the result is acceptable press
MENU and the display will read:
SAMPLESAMPLE - 0
CALCAL - 1 PRIMEPRIME - 2
You are now ready to measure samples.
6.2 Sample Cycle
With the SPORT calibrated as described, you can proceed to measure lactate
values in blood samples or plasma.
WARNING: If you calibrate with the Syringepet, measure with the Syringepet.
If you calibrate with the injector, measure with the injector. Otherwise, you
may get incorrect values.
REMEMBER: The Reference solution is only used in Auto mode. The
following sample cycle is illustrated as if the reference solution were indeed
being used. If you are operating in Manual mode ignore the messages relative to
the Reference solution. Press the MENU key until the Main menu appears:
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Run by pressing ENTER.
ENTER
SAMPLESAMPLE - 0
CALCAL - 1 PRIMEPRIME - 2
6 -8
Select the Sample cycle by pressing ENTER.
ENTER
The memory is limited to 150 results. You can continue to run samples while the
memory is full, but the results will not be saved. Stored results will have to be
deleted in order to make room available. The procedure for deleting stored
results is described later in this section. If the memory is full, the display will
read:
MEMORY FULL
STOPSTOP - 0 PROCEEDPROCEED - 1
Selecting Proceed will allow you to run the sample, but remember that the result
will not be stored. If you continue the display will read:
ENTER ID# XXX
When the "ID" display appears, enter the ID as a three digit number (example:
one is entered as 001; two hundred and thirteen as 213), then press ENTER,
ENTER and
this display will appear:
WAIT...
If the probe current is not within the expected range, you will see this display:
ERROR: BASELINE
PRESS MENU
If you see this display press MENU,
MENU then see Section 9 Troubleshooting.
If there is no problem, the display will read...
INJECT SAMPLE...
Inject the sample. When you remove the Syringepet or Injector, you will see
RUNNING...
STIR ON
RUNNING...
REF ON
6 -9
If the concentration of the sample exceeds the measurable limits of the
instrument, the following message will appear:
ERROR: OVERRANGE
WASHING...
If you see this display, no result will be stored or displayed, press MENU and
refer to Section 9 Troubleshooting.
If there is no problem, the display will read
#XXX XX.XXmmol/L
WASHING...
MM/DD/YY HH:MM
#XXX XX.XXmmol/L
The sample result will be displayed along with the ID#, date and time.
Press MENU to return to the Run menu. The previous result, ID#, date and
time, will be stored in memory until it is deleted
SAMPLESAMPLE - 0
CALCAL - 1 PRIMEPRIME - 2
6.3 Recalling Stored Results
The SPORT allows you to recall any stored result. Results may be recalled by
ID#, by date, or all results may be recalled in chronological order. The following
sections identify the different ways to do this.
Recalling All Sample Results
Go to the Main menu by pressing MENU until the display reads
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
6-10
Select Recall by pressing 1 , then ENTER.
ENTER
SAMPLES LCDLCD - 0
PRINTPRINT - 1 XMITXMIT - 2
To select viewing the results on the display press ENTER.
ENTER
DISPLAYDISPLAY - 0
DELETE ALLALL - 1
To select viewing the results on the display press ENTER.
ENTER
LIST ALLALL - 0
BY: DATEDATE - 1 IDID - 2
Select List All by pressing ENTER.
ENTER
Press ENTER.
ENTER The first sample result stored will appear.
MM/DD/YY HH:MM
#XXX X.XXmmol/L
Every time you press ENTER,
ENTER the next value stored will be displayed. You can
cycle, step by step, through all of the results stored in memory. The date, time
ID number will change as necessary to match each sample.
When all of the results have been displayed you will see this display:
NO SAMPLES
PRESS MENU
NOTE: It is possible to end the result recall at any point by pressing MENU.
MENU
Recalling Today's Sample Results
Go to the Main menu by pressing MENU until the display reads
RUNRUN - 0 RECALL
R ECALLECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Recall by pressing 1 , then ENTER.
ENTER
6-11
SAMPLES LCDLCD - 0
PRINTPRINT - 1 XMITXMIT - 2
To select viewing the results on the display press ENTER.
ENTER
DISPLAYDISPLAY - 0
DELETE ALLALL - 1
To select viewing the results on the display press ENTER.
ENTER
LIST ALLALL - 0
BY: DATEDATE - 1 IDID - 2
Select Date by pressing 1 , then ENTER.
ENTER
DATE MM/DD/YY
XX/XX/XX
NOTE: The current date is what will appear on the display.
Press ENTER.
ENTER The first sample result stored today will appear.
MM/DD/YY HH:MM
#XXX X.XXmmol/L
Every time you press ENTER,
ENTER the next value stored will be displayed. You can
cycle, step by step, through all of the results on this date. The ID number will
change as necessary to match each sample.
When all of the results have been displayed you will see this display:
NO SAMPLES
PRESS MENU
NOTE: It is possible to end the result recall at any point by pressing MENU.
MENU
Recalling a Result From Any Previous Date
Go to the Main menu.
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Recall by pressing 1 , then ENTER.
ENTER
6-12
SAMPLES LCDLCD - 0
PRINTPRINT - 1 XMITXMIT - 2
To select viewing the results on the display press ENTER.
ENTER
DISPLAYDISPLAY - 0
DELETE ALLALL - 1
To select viewing the results on the display press ENTER.
ENTER
LIST ALLALL - 0
BY: DATEDATE - 1 IDID - 2
Select Date by pressing 1 , then ENTER.
ENTER
DATE MM/DD/YY
XX/XX/XX
NOTE: Today's date will appear. Key in a date as described in Section 3.6
System Setup, then press ENTER.
ENTER
Example: January 17, 2003 is entered as 01 17 03 ENTER
The first sample result stored in memory on that date will be displayed.
MM/DD/YY HH:MM
#XXX X.XXmmol/L
Every time you press ENTER,
ENTER the next result stored will be displayed. You can
cycle, step by step, through all of the results on this date. The ID number will
change as necessary to match each sample.
When all of the results have been displayed you will see this display:
NO SAMPLES
PRESS MENU
NOTE: It is possible to end the result recall at any point by pressing MENU.
MENU
6-13
Recalling a Result By ID Number
Go to Main menu by pressing the MENU key until the display reads.
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Recall by pressing 1 , then ENTER.
ENTER
SAMPLES LCDLCD - 0
PRINTPRINT - 1 XMITXMIT - 2
To select viewing the results on the display press ENTER.
ENTER
DISPLAYDISPLAY - 0
DELETE ALLALL - 1
To select viewing the results on the display press E NTER.
NTER
LIST ALLALL - 0
BY: DATEDATE - 1 IDID - 2
Select ID by pressing 2 , then ENTER.
ENTER
ENTER ID# XXX
Enter the desired ID as a three digit number, from left to right, then press
ENTER.
ENTER
The first sample result stored in memory that has the number you've entered will
be displayed.
M/DD/YY HH:MM
#XXX X.XXmmol/L
Every time you press ENTER,
ENTER the next result stored will be displayed. You can
cycle, step by step, through all of the results with this ID#. The date and time
will change as necessary to match each sample.
When all of the results have been displayed you will see this display:
NO SAMPLES
PRESS MENU
6-14
NOTE: It is possible to end the result recall at any point by pressing MENU.
MENU
When you are finished, press MENU until the Main menu appears.
6.4 Printing Results
It is possible to print out results as you are running samples. This is possible
because the SPORT sends all of the result data over the infrared data link
automatically at the end of each sample cycle. All that is necessary is that the
printer be positioned correctly and turned on. Refer to Figure 3.6 to determine
printer positioning and alignment.
The following sections describe the actions required to print stored results.
Printing All Sample Results
Go to Main menu by pressing the MENU key until the display reads.
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Recall by pressing 1 , then ENTER.
ENTER
SAMPLES LCDLCD - 0
PRINTPRINT - 1 XMITXMIT - 2
Select Print by pressing 1 , then ENTER.
ENTER
PRINT ALLALL - 0
BY: DATEDATE - 1 IDID - 2
To print all results in memory, press ENTER.
ENTER The display will be active during
the printing process showing each result as it is printed. It is not possible to
halt the printing once it has started. Once finished the display will return to
the RECALL menu.
SAMPLES LCDLCD - 0
PRINTPRINT - 1 XMITXMIT - 2
Use the MENU key to return to the Main menu.
6-15
NOTE: The printer must be placed in the correct position and turned on in order
to receive the data. Refer to Figure 3.6 for the printer placement if you are
unsure.
Printing Today's Re
Results
sults
Go to Main menu by pressing the MENU key until the display reads.
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Recall by pressing 1 , then ENTER.
ENTER
SAMPLES LCDLCD - 0
PRINTPRINT - 1 XMITXMIT - 2
Select Print by pressing 1 , then ENTER.
ENTER
PRINT ALLALL - 0
BY: DATEDATE - 1 IDID - 2
Select Date by pressing 1 , then ENTER.
ENTER
DATE MM/DD/YY
XX/XX/XX
NOTE: The current date is what will appear on the display.
Press ENTER.
ENTER The display will be active during the printing process showing
each result as it is printed. It is not possible to halt the printing once it has
started. Once finished the display will return to the RECALL menu.
SAMPLES LCDLCD - 0
PRINTPRINT - 1 XMITXMIT - 2
Use the MENU key to return to the Main menu.
6-16
Printing Previous Days' Results
Open the Main menu.
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Recall by pressing 1 , then ENTER.
ENTER
SAMPLES LCDLCD - 0
PRINTPRINT - 1 XMITXMIT - 2
Select Print by pressing 1 , then ENTER.
ENTER
PRINT ALLALL - 0
BY: DATEDATE - 1 IDID - 2
Select Date by pressing 1 , then ENTER.
ENTER
DATE MM/DD/YY
XX/XX/XX
NOTE: Today's date will appear. Key in a date as described in Section 3.6
System Setup, then press ENTER.
ENTER
Example: January 17, 1998 is entered as 01 17 98 ENTER
The display will be active during the printing process showing each result as it
is printed. It is not possible to halt the printing once it has started. Once
finished the display will return to the RECALL menu.
SAMPLES LCDLCD - 0
PRINTPRINT - 1 XMITXMIT - 2
Use the MENU key to return to the Main menu.
Printing Results By ID Number
Go to the Main menu.
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
6-17
Select Recall by pressing 1 , then ENTER.
ENTER
SAMPLES LCDLCD - 0
PRINTPRINT - 1 XMITXMIT - 2
Select Print by pressing 1 , then ENTER.
ENTER
PRINT ALLALL - 0
BY: DATEDATE - 1 IDID - 2
Select ID by pressing 2 , then ENTER.
ENTER
ENTER ID# XXX
Enter the desired ID as a three digit number, from left to right, then press
ENTER.
ENTER
The display will be active during the printing process showing each result as it
is printed. It is not possible to halt the printing once it has started. Once
finished the display will return to the RECALL menu.
SAMPLES LCDLCD- 0
PRINTPRINT - 1 XMITXMIT - 2
Use the MENU key to return to the Main menu.
6.5 Transmitting Results Over the RS232 Communication Link.
NOTE: Be sure all the communication parameters have been set correctly. Refer
to Section 3 Instrument Setup.
Go to the Main menu.
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Recall by pressing 1 , then ENTER.
ENTER
SAMPLES LCDLCD - 0
PRINTPRINT - 1 XMITXMIT - 2
Select XMIT by pressing 2 , then ENTER.
ENTER
6-18
The display will be active during the transmitting process, flashing each result
as it is transmitted. All stored data will be transmitted and it is not possible to
halt the process once it has started. Once finished the display will return to
the RECALL menu.
SAMPLES LCDLCD - 0
PRINTPRINT- 1 XMITXMIT- 2
Use the MENU key to return to the Main menu.
6.6 Deleting Stored Results
There are two ways to delete results that have been stored in memory. The first
method described edits out results no longer required. This method requires that
the result that is to be deleted be recalled on the display to make sure that the
correct result is deleted.
The second method deletes all results.
Editing Results
Go to the Main menu by pressing MENU until the display reads
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Recall by pressing 1 , then ENTER.
ENTER
SAMPLES LCDLCD - 0
PRINTPRINT - 1 XMITXMIT - 2
To select viewing the results on the display press ENTER.
ENTER
DISPLAYDISPLAY - 0
DELETE ALLALL - 1
To select viewing the results on the display press ENTER.
ENTER
LIST ALLALL - 0
BY: DATEDATE - 1 IDID - 2
6-19
The results that need to be deleted can be displayed by date, by ID#, or all the
results may be displayed. To delete the displayed result, press the DELETE key
and the result will be deleted from the memory. The display will then step to the
next result. If you do not wish to delete this result, press ENTER and the next
result will be displayed.
Deleting All Results
Go to Main menu by pressing MENU until you see:
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Recall by pressing 1 , then ENTER.
ENTER
SAMPLES LCDLCD - 0
PRINTPRINT - 1 XMITXMIT - 2
To select viewing the results on the display press ENTER.
ENTER
DISPLAYDISPLAY - 0
DELETE ALLALL - 1
To delete all of the stored results press 1 , then ENTER.
ENTER
ARE YOU SURE?
NONO - 0 YESYES - 1
To delete all results press 1 , then ENTER.
ENTER The display will read
RESULTS DELETED
"PRESS MENU"
If you chose not to delete all the results, pressing MENU will return you to the
previous menu. Pressing ENTER will display
RESULTS OK
"PRESS MENU"
Press MENU to return to the Main menu.
6-20
6.7 Daily Operational Checks
To assure that your SPORT is operating properly, check the membrane integrity
and the linearity every day.
We recommend that you keep an instrument logbook in which dates and lot
numbers of reagents are recorded, along with a record of daily operational
checks and other relevant information.
Membrane Integrit
Integrity
y Test
Use YSI 2363 Potassium Ferrocyanide (FCN) to determine if your membranes
are intact. This solution is packaged in your starter kit, and may also be ordered
separately. With the instrument in SAMPLE mode, inject 25 microliters of the
solution with whichever injection device was used for calibration.
The reading should be below 0.3 mmol/L (2.7 mg/dL). If it is greater, repeat the
test. If it's still too high, change the membrane.
Linearity Test
Use YSI 1530 30 mmol/L or YSI 2328 15 mmol/L Lactate Standard to check the
linearity of the sensor. These standards are packaged in your starter kit, and also
may be ordered separately.
Acceptable Ranges:
YSI 2328 14.30 to 15.70 mmol/L (128.3 to 141.7 mg/dL)
YSI 1530 28.50 to 31.50 mmol/L (256.5 to 283.5 mg/dL)
6-21
6-22
7. Instrument Diagnostics
Diagnostic routines in the software provide troubleshooting capability not only
in manufacturing, but also in field service and user maintenance.
These routines check the following elements of the electronic and fluid systems.
•
Probe current. Buffer and reference fluid cycles are independently accessible to
monitor probe current change.
•
Sample chamber temperature
•
Injection switch status
•
Motors. The pump motors and stir motor can be run separately or
simultaneously.
•
RS232. A test data string is sent over the serial communications link.
•
Printer. A print test form is sent over the infrared data link.
•
Keypad. Each key can be verified for performance.
•
Display. A diagnostic routine checks every LCD segment for failure.
•
Battery. Battery voltage can be monitored on the display.
7.1 Checking Probe Current
Go to Main menu by pressing MENU until you see:
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Diagnostics by pressing 3 , then ENTER.
ENTER
PROBEPROBE - 0 TEMPTEMP - 1
INJ SWSW - 2 MOREMORE - 3
Select Probe by pressing ENTER.
ENTER
PROBE
PRO BEBE - 0
REFREF - 1 BUFBUF - 2
To display the probe current, press ENTER. The display will read:
7 -1
WAIT...
PROBE X.XX nA
The display will update every few seconds. To leave this condition, press
MENU. The display will read:
PROBEPROBE- 0
REFREF - 1 BUFBUF - 2
You can also run either the reference or buffer pumps and monitor the change in
probe current. To do this press the desired numerical key and then ENTER,
ENTER the
display will read:
PROBE X.XX nA
The display will update every few seconds. To leave this mode, press MENU.
MENU
PROBEPROBE - 0
R EFEF - 1 BUFBUF - 2
Press MENU until main menu appears.
7.2 Checking Sample Chamber Temperature
Go to Main menu by pressing MENU until you see:
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Diagnostics by pressing 3 , then ENTER.
ENTER
PROBEPROBE - 0 TEMPTEMP - 1
INJ SWSW - 2 MOREMORE - 3
7 -2
Select Temperature by pressing 1 , then ENTER.
ENTER The display will show the
current chamber temperature.
CHAMBER TEMP XX.XX
C
Press MENU to return to the Main menu.
7.3 Checking the Sample Chamber Injection Switch Status
Go to Main menu by pressing MENU until you see:
RUNRUN - 0 RECALL
RECA LLLL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Diagnostics by pressing 3 , then ENTER.
ENTER
PROBEPROBE - 0 TEMPTEMP - 1
INJ SWSW - 2 MOREMORE - 3
Select Injection Switch by pressing 2 , then ENTER.
ENTER
INJECTION SWITCH
OFF
Inserting an injection device will trigger the switch and the change in state will
be indicated (ON / OFF) on the display. Press MENU to return to the Main
menu.
7.4 Turning the Stir Motor On and Off
Go to Main menu by pressing MENU until you see:
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Diagnostics by pressing 3 , then ENTER.
ENTER
PROBEPROBE - 0 TEMPTEMP - 1
INJ SWSW - 2 MOREMORE - 3
7 -3
Select More by pressing 3 , then ENTER.
ENTER
STIRSTIR - 0 PUMPSPUMPS - 1
PRINTPRINT - 2 MOREMORE - 3
Select Stir by pressing ENTER.
ENTER
STIR OFFOFF - 0 ONON - 1
Turn the stir motor on or off by pressing the appropriate number key and then
ENTER.
ENTER Press MENU to return to the Main menu.
7.5 Turning Pump Motors On and Off
Go to Main menu by pressing MENU until you see:
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Diagnostics by pressing 3 , then ENTER.
ENTER
PROBEPROBE - 0 TEMPTEMP - 1
INJ SWSW - 2 MOREMORE - 3
Select More by pressing 3 , then ENTER.
ENTER
STIRSTIR - 0 PUMPSPUMPS - 1
PRINTPRINT - 2 MOREMORE - 3
Select Pumps by pressing 1 , then ENTER.
ENTER The display will read:
BUF OFFOFF - 0 ONON - 1
REF OFFOFF - 2 ONON - 3
Turn the pump motors on or off by pressing the appropriate number key and
then ENTER.
ENTER Press MENU to return to the Main menu.
7 -4
7.6 Sending a Test Print String Over the Infrared Data Link
Go to Main menu by pressing MENU until you see:
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP- 2 DIAGDIAG- 3
Select Diagnostics by pressing 3 , then ENTER.
ENTER
PROBEPROBE - 0 TEMPTEMP - 1
INJ SWSW - 2 MOREMORE - 3
Select More by pressing 3 , then ENTER.
ENTER
STIRSTIR - 0 PUMPSPUMPS - 1
PRINTPRINT - 2 MOREMORE - 3
Select Print by pressing 2 , then ENTER.
ENTER
TEST PRINT
PRINT-- 0
TEST RS232RS232 - 1
Press ENTER to send a test form over the infrared data link.
PRINTING…
TEST PRINT
PRINT-- 0
TEST RS232RS232 - 1
Press MENU to return to the Main menu.
7.7 Sending a Test Data String Over the RS232 Data Link
Go to Main menu by pressing MENU until you see:
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP- 2 DIAGDIAG- 3
7 -5
Select Diagnostics by pressing 3 , then ENTER.
ENTER
PROBEPROBE - 0 TEMPTEMP - 1
INJ SWSW - 2 MOREMORE - 3
Select More by pressing 3 , then ENTER.
ENTER
STIRSTIR - 0 PUMPSPUMPS - 1
PRINTPRINT - 2 MOREMORE - 3
Select Print by pressing 2 , then E NTER.
NTER
TEST PRINT
PRINT-- 0
TEST RS232RS232 - 1
Press 1, then ENTER to send a test data string over the RS232 data link.
XMITTING…
XXXXXXXXXXXXXX
TEST PRINTPRINT - 0
TEST RS232RS232- 1
Press MENU to return to the Main menu.
7.8 Testing the Keypad
Go to Main menu by pressing MENU until you see:
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Diagnostics by pressing 3 , then ENTER.
ENTER
PROBEPROBE - 0 TEMPTEMP - 1
INJ SWSW - 2 MOREMORE - 3
Select More by pressing 3 , then ENTER.
ENTER
7 -6
STIRSTIR - 0 PUMPSPUMPS - 1
PRINTPRINT- 2 MOREMORE- 3
Select More by pressing 3 , then ENTER.
ENTER
KEYSKEYS- 0 DISPLAYDISPLAY- 1
VER ## - 2 MOREMORE - 3
Select Keys by pressing ENTER.
ENTER The display will be empty except for the
cursor in the upper left hand corner. The display will now respond to each key
pressed by showing the description of the key on the display. Press MENU to
return to the Main menu.
7.9 Testing the Display
Go to Main menu by pressing MENU until you see:
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Diagnostics by pressing 3 , then ENTER.
ENTER
PROBEPROBE - 0 TEMPTEMP - 1
INJ SWSW - 2 MOREMORE - 3
Select More by pressing 3 , then ENTER.
ENTER
STIRSTIR - 0 PUMPSPUMPS - 1
PRINTPRINT - 2 MOREMORE - 3
Select More by pressing 3 , then ENTER.
ENTER
KEYSKEYS - 0 DISPLAYDISPLAY - 1
VER ## - 2 MOREMORE - 3
Press 1 , then ENTER to initiate the display test routine. Pressing CANCEL will
halt the test at any time. The test routine consists of displaying multiple
character sets at three different contrast settings and also lighting every LCD
segment.
Press MENU to return to the Main menu.
7 -7
7.10 Checking the Software Version Number
It will be necessary to communicate the software version number to a service
technician if a call is required. Press MENU until you return to the Main menu.
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
Select Diagnostics by pressing 3 , then ENTER.
ENTER
PROBEPROBE - 0 TEMPTEMP - 1
INJ SWSW - 2 MOREMORE - 3
Select More by pressing 3 , then ENTER.
ENTER
STIRSTIR - 0 PUMPSPUMPS - 1
PRINTPRINT- 2 MOREMORE- 3
Select More by pressing 3 , then ENTER.
ENTER
KEYSKEYS- 0 DISPLAY
DISPLAYPLAY - 1
VER ## - 2 MOREMORE - 3
Select Version by pressing 2 , then ENTER.
ENTER The software version number will
be displayed.
VERSION X.XX LAC
PRESS MENU
Press MENU to return to the Main menu.
7.11 Checking the Battery Voltage
To display the "non-loaded" battery voltage, press MENU until you return to the
Main menu.
The "loaded" voltage will typically be about 0.06 volt less.
RUNRUN - 0 RECALLRECALL - 1
SETUPSETUP - 2 DIAGDIAG - 3
7 -8
Select Diagnostics by pressing 3 , then ENTER.
ENTER
PROBEPROBE - 0 TEMPTEMP - 1
INJ SWSW - 2 MOREMORE - 3
Select More by pressing 3 , then ENTER.
ENTER
STIR
STI R - 0 PUMPSPUMPS - 1
PRINTPRINT - 2 MOREMORE - 3
Select More by pressing 3 , then ENTER.
ENTER
KEYSKEYS - 0 DISPLAYDISPLAY - 1
VER ## - 2 MOREMORE - 3
Select More by pressing 3 , then ENTER.
ENTER
BATTERYBATTERY- 0
Press ENTER to display the nonnon- loaded battery voltage.
BATTERY XX.XX
XX
The display will update every few seconds. Press MENU to return to the Main
menu. The number on the bottom line is the number of times the battery voltage
reading is updated and will clear automatically when leaving this menu.
Note: Typically the loaded voltage at which the actual battery checks are done is
lower by 0.10 volts.
7 -9
7-10
8. Maintenance
Turn the instrument power off during maintenance. This will prevent any
accidental activation of the fluid pumps which could cause spills or air in the
tubing.
8.1 Daily Maintenance
Certain procedures are to be followed each day that the SPORT is in use.
Empty the Waste Bottles
WARNING: The waste contents of the SPORT are considered a biohazard
and must be disposed of accordingly. The waste bottle should be cleaned with
a disinfecting agent. The reagents used in the instrument are non-toxic and
consist of a phosphate salt buffer, lactate standards and the detergent Triton X100.
Check Buffer and Reference Bottle Levels
The SPORT does not sense fluid levels automatically, so you have to look to see
that you have enough. If you run out and don't realize it, you'll get wrong data
and you may lose samples.
Replace the Buffer and Reference solutions if they are low or have been in the
instrument longer than a week. Follow the instructions in Section 3.3 Reagent
Preparation.
After a solution change, prime the fluid system. Select RUNRUN - 0 from the Main
menu. Selecting PRIMEPRIME - 2 will run predetermined amounts of reference and
buffer solutions in order to fill all the supply lines completely.
NOTE: There is usually air in the waste tubing. This does not affect
performance.
Check for Air Bubbles in Chamber
Examine the sample chamber for any air bubbles that may have stuck to the
membrane and/or stir bar. Bubbles that do not flush out of the chamber can give
you artificially high lactate readings. Refer to Section 9 Troubleshooting for
directions in removing air bubbles.
8 -1
Check for Leaks
Examine the tubing for leaks and check the sample chamber for large air
bubbles, which may be caused by either loose connections or worn pump tubing.
Refer to Section 8.6 Tubing Replacement if the tubing needs to be replaced.
Clean up spills
Spills should be cleaned up immediately to prevent corrosion and biohazard
buildup. Blood spills must be carefully and safely removed. If there is any
evidence of salt build-up around the sample chamber, disassemble and clean the
chamber as described in Section 8.3.
8.2 Preventative Maintenance
The YSI 1503 Preventative Maintenance Kit contains all the supplies necessary
to keep your SPORT operating properly. Perform all the maintenance procedures
in Section 8.3 through 8.7 every 6 months, or as required.
IMPORTANT: The Waste bottles, both reagent bottles, the 500mL mixing
bottle and the 3cc Syringe provided in the YSI 1504 Starter Kit should be
cleaned every week with a disinfecting agent. The bottles and Syringe should be
rinsed completely with distilled water after cleaning to remove any disinfecting
residue, which would cause membrane failure if it got into the system.
8.3 Cleaning the Sample Chamber
Clean the sample chamber every month.
Unscrew the probes from their housings. If the membrane has passed the tests in
Section 6.7, you don't have to replace it, but you shouldn't let it dry out. If the
chamber is off the instrument for more than 15 minutes, the membrane should
be replaced.
Disconnect the supply and waste line tubing from the fittings on the sample
chamber manifold.
Remove the two screws securing the injection block to the top of the sample
chamber and move it to one side. Remove the 2 screws securing the sample
chamber to the case panel.
Remove the screw holding the manifold to the chamber. Be careful not to lose
the two O-rings that seal the connection. Replace them if necessary.
Be careful not to discard the small magnetic stir bar. An extra one is supplied in
your maintenance kit. Clean the chamber with the appropriate disinfecting
agent, then flush it with copious amounts of warm water, followed by a distilled
water rinse.
8 -2
Reassemble the chamber and manifold and attach the assembly to the case
panel. Reattach the injection block to the top of the sample chamber.
Remember to return the stir bar to the chamber. Install new O-rings or a new
membrane if necessary, and install the probes. Extra O-rings are supplied in
your Preventative Maintenance Kit.
8.4 Membrane Replacement
To assure proper performance and prevent unexpected downtime, we
recommend that you replace lactate membranes every 14 days. See Section 3.4
for membrane replacement procedure.
8.5 Probe Cleaning
With normal use, enzyme sensors may become fouled and cease to operate in a
normal mode. A fouled sensor’s output current will decrease and calibration
may become unstable.
Since the severity of fouling will vary, listed below are methods of cleaning
which should be matched to the cleaning needs. Follow the steps carefully and
in order.
Sensor Maintenance
It is necessary to maintenance the enzyme sensor when the 1503 PM kit is
installed and periodically as needed.
1. Remove the enzyme membrane and hold the probe with the electrodes facing
up.
2. Wad a small portion of a lint free tissue and wet it with 70% isopropyl
alcohol.
3. Using your thumb, press the alcohol soaked wad against the probe’s surface
and rotate the probe back and forth.
4. Rinse the sensor with reagent grade water and return the sensor to normal
service.
Sensor Cleaning an
and
d Reconditioning
The following procedure is recommended to be used only when the enzyme
sensor stops operating normally and the above maintenance procedure is
ineffective. This is not a routine maintenance procedure.
1. With the membrane removed, immerse the sensor in a 14% solution of
ammonia for 3 minutes.
2. Immediately after soaking, rinse the sensor with reagent grade water for 3-5
minutes. It is important that all the residual ammonia is removed.
8 -3
3. Prepare a small amount of 0.5% sodium hypochlorite solution.
4. Immerse the sensor in the solution for 30 - 45 seconds. Check
periodically...sensor should darken, but do not allow it to become black.
5. Remove the sensor and immediately rinse it with reagent water for at least 2
minutes.
6. Install a new membrane and return the probe to service.
The procedure below is only recommended as a last resort for revitalizing a
sensor after the above cleaning procedure has been unsuccessfully attempted.
WARNING: The following procedure may cause
permanent damage to the enzyme sensor.
1. Using a clean new pencil eraser, carefully, rub the center electrode
(platinum) of the sensor. Do not rub the epoxy between the two electrodes
or the outer silver electrode.
2. To remove any eraser dust or residual, rinse sensor with 70% isopropyl
alcohol and then reagent grade water.
3. Prepare a small amount of 0.5% sodium hypochlorite solution.
4. Immerse the sensor in the solution for about 45 seconds.
5. Remove the sensor and immediately rinse it with reagent grade water for at
least 2 minutes.
6. Install a new membrane and return the probe to service.
NOTE: Several hours may be required for the sensor to stabilize after cleaning.
8.6 Tubing Replacement
The buffer and reference solutions are moved through the SPORT with
peristaltic tubing pumps. Tubing life depends on how much the instrument is
used. Replace all of the SPORT tubing at least every 6 months. Any tube that
becomes loose at the fittings should be replaced immediately.
Remove the screws holding the buffer and calibrator pump to the case panel.
Disconnect and remove all the instrument tubing. New tubing is supplied in the
Preventive Maintenance Kit. Connect the new tubing as shown in Figure 8-1.
When installing new pump tubing, first thread the tube through the pump head
and install the roller assembly (see Figure 8-2). It will help to twist the roller
assembly as you install it. It should snap into place. Remount the pump to the
case panel. Remember to install the tube bushings on both the inlet and outlet
side of the pumps.
8 -4
Waste
Supply
Waste
Supply
Figure 8 - 1 SPORT Tubing Routing
WARNING: It is extremely important that the correct size tubing be used for
the supply and waste pumps. Switching the two will result in sample chamber
overflow.
Supply pump tubing size.....5/32" OD x 1/32" wall
Waste pump tubing size......3/16" OD x 1/32" wall
Upper Body
Roller Body
Lower Body
Make sure tubing
is not twisted inside
body before assembly
Figure 8 - 2 SPORT Pump Assembly
8 -5
8.7 Injection Port Seal Replacement
An injection port seal is included with your instrument. It is used during
transportation and long term storage. Replace the plug and O-ring on it every six
months, because they can deteriorate and allow chamber fluid leakage. Extra Orings and plugs are supplied in the Preventative Maintenance Kit.
8.8 Recharging the Battery
A fully charged battery will provide a minimum of 8 hours* operating time. At
this time or shortly thereafter a warning will be displayed after each sample
cycle alerting you to a "low battery " condition.
To recharge the battery, switch the power off, plug the wall charging unit into an
available AC outlet and connect the other end to the instrument interface panel
at the Power Receptacle which is labeled CHARGE. The charger is designed to
fully charge the SPORT battery over a period of 8 hours, disconnecting the
charger prior to this will impact the amount of time available for battery only
operation. Once the battery is fully charged, the red indicator lamp on the
charger will flicker on and off rapidly. This is the indication that the charger is
floating. An overnight charge should be more than adequate to insure a full day
of "battery only testing" the next day. The SPORT may be turned back on
immediately or left with the power off. The battery will be recharged in either
condition. Turning on the power will allow immediate continuation once the
probe has restabilized.
WARNING: Always make sure the sport power is turned
off prior to connecting the battery charger. Failure to do
this could result in stored data corruption if the battery
has been discharged to a very low level.
* Refer to Appendix F–Temperature Effect on Operation for additional
information.
8.9 Storing the SPORT
Electrical System
We recommend that while storing the SPORT, you leave the unit on charge. This
will guarantee that the next time the SPORT is needed, the battery will be fully
charged and ready for a full day of use if necessary. Failure to do this could
result in a battery that is incapable of retaining a charge sufficient enough to
guarantee 8 hours of use. This is because even though the battery is hardware
disconnected at a level of 11.5 volts it is possible due to the normal discharging
8 -6
of the battery itself that the capacity of the battery will be permanently
compromised if the unit remains off charge for several months.
YSI recommends that prior to storing, the battery should
be fully charged (8 hours minimum) and the power turned
off.
Fluid System
If the SPORT is to be inactive for longer than a week, we recommend that the
whole system be flushed completely out with either deionized or distilled water.
This will prevent any of the salts that are present in the buffer and reference
solutions from crystallizing and blocking the fluid path.
This can be done quite simply by first emptying all of the fluid bottles and then
rinsing and filling the supply bottles with distilled or deionized water. The
system can then be either cycled using the priming function available in RUN
mode, or it may be done through DIAGNOSTICS using the individual
functions.
Storage of the unit for less than a week can be done with the buffer and
reference fluids in place.
NOTE: It is possible that after a period of storage, depending on the tubing
condition, the tubing inside the pump may stick closed preventing fluid flow.
Depending on whether it happens to the waste or supply tubing, the chamber
may overflow or it simply may not fill correctly when attempting to run the unit
for the first time. Should either of these things happen, refer to Section 9
Troubleshooting, for instructions on how to correct the problem.
8 -7
9. Troubleshooting
This section deals with identifying and correcting problems that interfere with
the performance of the SPORT. The fault messages that the instrument may
display are listed, then a brief explanation of the fault is given. Following each
fault is a list of possible causes with recommended corrective actions.
It is important that a systematic approach is taken to identifying the cause of an
instrument failure. The first step is to accumulate as much pertinent information
as possible.
•
Review the printed reports for trends in data and errors. (This is only possible if
the optional printer is being used while running samples. Refer to Section 3.8
Printer Option, for an explanation of the printed format.)
•
Check reagent and membrane installation dates. Compare the elapsed times with
the recommended times.
•
Look and listen for problems (unusually noisy components, loose connections,
fluid leaks, air bubbles in the sample chamber, salt build-ups, etc.).
•
Review Section 7 Instrument Diagnostics to become familiar with testing
individual components of the SPORT.
•
Use the troubleshooting chart in this section to assist you in identifying the
problem, then use the chart to guide you to a corrective action.
If the problem cannot be resolved, contact YSI Customer Service (address and
phone number in Appendix I–Warranty and Shipping Information). When
communicating with service personnel, please indicate the serial number of the
instrument and if necessary the accessory products. If writing or transmitting a
FAX for assistance, please include a thorough description of the problem(s) and
copies of the printed reports if possible.
If it becomes necessary to return the instrument to YSI for servicing please refer
to Appendix I–Warranty and Shipping Information for instructions.
9 -1
9.1 Fault Messages
Data System Faults
ROM IS NEW
PRESS ENTER
An internal test of the most recent and current EPROM checksum data is done
and if there is a discrepancy this message is displayed. This fault is generated
when the software has been replaced with a different version. Pressing ENTER
will display the following message.
OK TO LOSE DATA
NONO - 0 YES - 1
Two options exist at this point, one is to select NO, save your data and decide
not to continue. Choosing this option will display the following:
POWER DOWN
CONSULT MANUAL
We recommend at this point that you contact YSI Customer Service as this is a
system failure that may require returning the instrument.
The second option is to select YES which will reformat the SPORT memory.
The software will attempt to correct itself but in doing so ALL stored data will
be lost. If YES was selected, the stir speed, date and time must also be reset.
Refer to Section 3 Instrument Setup for more information if necessary.
IMPORTANT: Any previously stored sample results will be permanently
erased by selecting YES.
BAD SYS VARS
PRESS ENTER
A check is done of all data system variables after each sample cycle, if there is a
discrepancy this message will be displayed.
OK TO LOSE DATA?
NO - 0 YES - 1
Two options exist at this point, one is to select NO and save your data and
decide not to continue. Choosing this option will display the following:
POWER DOWN
CONSULT MANUAL
9 -2
We recommend at this point that you contact YSI Customer Service as this is a
system failure that may require returning the instrument.
The second option is to select YES which will reformat the SPORT memory.
The software will attempt to correct itself but in doing so ALL stored data will
be lost. If YES was selected, the stir speed, date and time must also be reset.
Refer to Section 3 Instrument Setup for more information if necessary.
IMPORTANT: Any previously stored sample results will be permanently erased
by selecting YES.
BAD RAM DATA
PRESS ENTER
A check is done of all stored data after each sample cycle, if there is a
discrepancy this message will be displayed.
OK TO LOSE DATA?
NO - 0 YES - 1
Two options exist at this point, one is to select NO and save your data and
decide not to continue. Choosing this option will display the following:
POWER DOWN
CONSULT MANUAL
We recommend at this point that you contact YSI Customer Service as this is a
system failure that may require returning the instrument.
The second option is to select YES which will reformat the SPORT memory.
The software will attempt to correct itself but in doing so ALL stored data will
be lost. If YES was selected, the stir speed, date and time must also be reset.
Refer to Section 3 Instrument Setup for more information if necessary.
IMPORTANT: Any previously stored sample results will be permanently erased
by selecting YES.
INJECT TIMEOUT
PRESS MENU
This message will be displayed if, after being prompted to inject either a
calibrator or a sample, 5 minutes passes.
Pressing MENU will move you to the previous menu from where you will be
allowed to continue once more.
9 -3
Battery Faults
LOW BATTERY
PRESS ON
A check of the battery voltage is done during the sample cycle. At 12.1 volts this
message is displayed. Sampling will not be prohibited at this point, typically
there is enough battery reserve at this point to continue for at least 1 hour of
continuous testing (minimum of 40 samples). Press the ON key to clear the
message and allow operation of the instrument.
CONNECT CHARGER
PRESS ON
This message is displayed when the level drops below 12.0 volts. At this point
in order to continue you must connect the battery charger. The software will not
allow you to continue sampling though until the battery has been recharged to a
level of 12.8 volts. This is done to protect the discharge/recharge capacity of the
battery and insure the maximum amount of good usable battery life.
Enzyme Sensor Faults
ERROR: BASELINE
PRESS MENU
This fault is generated because the probe baseline current is above the allowable
starting point of 6 nanoamps. It is common to see this fault if you try to run
samples too soon after turning the instrument on or after putting on a new
membrane. Remember that new membranes need time to break in.
9 -4
•
POSSIBLE CAUSE: Reference solution in sample chamber.
•
CORRECTIVE ACTION: Enter PROBE diagnostics and cycle the buffer pump.
•
SECTION: 7.1
•
POSSIBLE CAUSE: Tubing routed incorrectly through peristaltic pumps.
•
CORRECTIVE ACTION: Check tubing routing and verify fluid flow path.
•
SECTION: 8.6
•
POSSIBLE CAUSE: A sample has been injected into the sample chamber at the
improper time.
•
CORRECTIVE ACTION: Enter PROBE diagnostics and cycle the buffer pump.
•
SECTION: 7.1
•
POSSIBLE CAUSE: Pinched, plugged, leaking or disconnected tubing.
•
CORRECTIVE ACTION: Fix or replace tubing.
•
SECTION: 8.6
•
POSSIBLE CAUSE: Buffer pump not running.
•
CORRECTIVE ACTION: Enter PUMP diagnostics and verify buffer pump
performance.
•
SECTION: 7.5
•
POSSIBLE CAUSE: Buffer pump not performing properly.
•
CORRECTIVE ACTION: Replace tubing.
•
SECTION: 8.6
•
POSSIBLE CAUSE: Stir bar not present.
•
CORRECTIVE ACTION: Disassemble chamber and reinstall stir bar.
•
SECTION: 8.3
•
POSSIBLE CAUSE: Stir bar not spinning.
•
CORRECTIVE ACTION: Enter STIR diagnostics and check motor
performance.
•
SECTION: 7.4
•
POSSIBLE CAUSE: Failing enzyme membrane.
•
CORRECTIVE ACTION: Perform daily operational checks and replace if
necessary.
•
SECTION: 6.7
9 -5
•
POSSIBLE CAUSE: Newly installed enzyme membrane.
•
CORRECTIVE ACTION: Enter PROBE diagnostics and check probe current.
•
SECTION: 7.1
•
POSSIBLE CAUSE: Newly installed enzyme probe.
•
CORRECTIVE ACTION: Enter PROBE diagnostics and check probe current.
•
SECTION: 7.1
•
POSSIBLE CAUSE: Enzyme probe surface fouled.
•
CORRECTIVE ACTION: Clean probe surface.
•
SECTION: 8.5
•
POSSIBLE CAUSE: Temperature probe (auxiliary electrode) surface fouled.
•
CORRECTIVE ACTION: Clean probe surface.
•
SECTION: 8.5
•
POSSIBLE CAUSE: Main power disruption.
•
CORRECTIVE ACTION: Enter PROBE diagnostics and check probe current.
•
SECTION: 7.1
ERROR: MEMB SENS
PRESS MENU
This fault is generated only during the Calibration cycle. Because a sample of a
known concentration (5 mmol/L Lactate Standard) has been injected, the current
generated should fall within an expected range (5-45 nA). If the actual current is
higher or lower than expected the above message will be displayed.
9 -6
•
POSSIBLE CAUSE: Improper sample injection.
•
CORRECTIVE ACTION: Repeat procedure.
•
SECTION: 4.1
•
POSSIBLE CAUSE: Failing enzyme membrane.
•
CORRECTIVE ACTION: Perform daily operational checks and replace if
necessary.
•
SECTION: 6.7
•
POSSIBLE CAUSE: Pinched, plugged, leaking or disconnected tubing.
•
CORRECTIVE ACTION: Fix or replace tubing.
•
SECTION: 8.6
•
POSSIBLE CAUSE: Calibrator solution out of spec: contaminated or in use for
more than 30 days.
•
CORRECTIVE ACTION: Replace Calibrator solution and repeat procedure.
•
POSSIBLE CAUSE: Enzyme probe surface fouled.
•
CORRECTIVE ACTION: Clean probe surface.
•
SECTION: 8.5
•
POSSIBLE CAUSE: Temperature probe (auxiliary electrode) surface fouled.
•
CORRECTIVE ACTION: Clean probe surface.
•
SECTION: 8.5
•
POSSIBLE CAUSE: Stir bar not present.
•
CORRECTIVE ACTION: Disassemble chamber and reinstall stir bar.
•
SECTION: 8.3
•
POSSIBLE CAUSE: Stir bar not spinning.
•
CORRECTIVE ACTION: Enter STIR diagnostics and check motor
performance.
•
SECTION: 7.4
ERROR:OVERERROR:OVER - RANGE
PRESS MENU
9 -7
This fault is generated when the sample concentration exceeds the upper limit of
the dynamic range (30 mmol). No result will be stored in memory.
•
POSSIBLE CAUSE: Sample concentration is too high, resulting in high probe
current.
•
CORRECTIVE ACTION: Dilute sample 1:1 with the same buffer solution being
used in the instrument and repeat.
•
SECTION: 6
•
POSSIBLE CAUSE: Excessive sample carryover on the outside of the
Syringepet needle or capillary tube.
•
CORRECTIVE ACTION: Repeat sampling procedure making sure to wipe
excess sample from the outside of the injection device.
•
SECTION: 6
Temperature Faults
ERR: BAD TEMP
PRESS MENU
The instrument will work only over the specified temperature range (5-45°C).
•
POSSIBLE CAUSE: Ambient temperature too cold or hot.
•
CORRECTIVE ACTION: Operate at ambient temperatures between 5 and 45°C.
TEMP UNSTABLE "PRESS
MENU"
•
POSSIBLE CAUSE: If this fault appears after a sample cycle and no result has
been displayed the sample chamber temperature is changing too rapidly, (more
than 3°C from the sample cycle to the reference cycle).
•
CORRECTIVE ACTION: Allow the instrument supply fluids to equilibrate at
the ambient temperature.
•
CORRECTIVE ACTION: Place the SPORT out of direct sunlight and allow
several minutes for stabilization.
TEMP CHANGE
FLUSHING...
9 -8
This display will appear prior to any sample or calibrator injection if the sample
chamber temperature has changed more than 1°C since the last wash cycle. A
full wash cycle will be automatically initiated to insure that the contents of the
chamber are not artificially warmed because of direct sunlight or other
phenomena. Once the wash cycle is complete the chamber will be ready for
injection.
Fluid System Faults
ERROR: REF
CONCENTRATION LO
This warning is generated when the Reference solution concentration is
calculated to be less than 80% of the injected Calibration standard (YSI 2327 5
mmol/L Lactate Std.). It is possible that significantly high or low Reference
solution concentrations can have a negative effect on the performance of the
SPORT at the upper most part of the dynamic range. If this fault is encountered
during an attempt to calibrate the instrument, replace the Reference solution and
repeat the calibration process.
ERROR: REF
CONCENTRATION HI
This warning is generated when the Reference solution concentration is
calculated to be greater than 120% of the injected Calibration standard (YSI
2327 5 mmol/L Lactate Std.). It is possible that significantly high or low
Reference solution concentrations can have a negative effect on the performance
of the SPORT at the upper most part of the dynamic range. If this fault is
encountered during an attempt to calibrate the instrument, replace the Reference
solution and repeat the calibration process.
UNSTABLE REFCUR
PRESS MENU
This fault can be generated in two different scenarios. One during the calibration
process, the second during a sampling cycle.
9 -9
If it occurs during calibration it is because the measured reference current after
the calibrator injection is different from the measured reference current after the
linearity injection by more than 5%.
If it occurs during sampling it is because the measured reference current after
the sample injection is different from the measured reference current after the
original calibration injection by more than 20%.
•
POSSIBLE CAUSE: Probe and membrane not stabilized as a result of recent
power up or power interruption.
•
CORRECTIVE ACTION: Enter PROBE Diagnostics and check to see if current
is stable or falling. Allow to stabilize then re-calibrate.
•
SECTION: 7.1
•
POSSIBLE CAUSE: Supply tubing crimped prohibiting reference solution flow
to the chamber.
•
CORRECTIVE ACTION: Visually inspect all pump tubing.
9.2 Possible Failure Conditions
The following are possible failures that may go undetected unless confirmed
visually but can affect instrument performance.
Air bubble in the sample chamber
9-10
•
POSSIBLE CAUSE: Loose fittings on supply bottles.
•
CORRECTIVE ACTION: Tighten fittings.
•
POSSIBLE CAUSE: Supply line check valves stuck open.
•
CORRECTIVE ACTION: Flush or replace check valves.
•
POSSIBLE CAUSE: Tubing worn or stretched at connections.
•
CORRECTIVE ACTION: Replace tubing.
•
SECTION: 8.6
•
POSSIBLE CAUSE: Air bubble stuck to stir bar.
•
CORRECTIVE ACTION: Go to stir bar setup and increase speed until the stir
bar tumbles (** See below). Reset spin speed.
•
SECTION: 3.6
** The stir bar is driven by a spinning magnetic coupling, if the speed is
increased the coupling will be interrupted and the stir bar will tumble and
vibrate fairly violently. If bubble will still not release, take a small diameter pin
or other device (paper clip works well) and stick it into the sample chamber
through the injection port and interrupt the stir bar motion. The bubble should
release itself at this point.
•
POSSIBLE CAUSE: Buffer and/or Reference tubing line in pumps crimped
shut.
•
CORRECTIVE ACTION: Disassemble pumps and open tubing or replace if
more than 6 months since last replaced.
•
SECTION: 8.6
Buffer and/or Reference pump fail to pump fluid.
•
POSSIBLE CAUSE: Tubing line in pumps crimped or stuck shut.
•
CORRECTIVE ACTION: Disassemble pumps and open tubing or replace if
more than 6 months since last replaced.
•
SECTION: 8.6
•
POSSIBLE CAUSE: Supply line blocked.
•
CORRECTIVE ACTION: Clear tubing or replace.
•
SECTION: 8.6
•
POSSIBLE CAUSE: Supply line check valve stuck open.
•
CORRECTIVE ACTION: Flush check valve or replace.
•
POSSIBLE CAUSE: Bottle vent plugged, causing negative pressure in the
supply bottle.
•
CORRECTIVE ACTION: Loosen supply bottle cap to verify, clear blockage or
replace vent.
•
POSSIBLE CAUSE: Sample chamber inlet blocked.
•
CORRECTIVE ACTION: Disassemble chamber, check for blockage in inlet
port (lower).
•
SECTION: 8.3
9-11
Sample chamber overflows through the injection port.
•
POSSIBLE CAUSE: Waste tubing line in pumps crimped or stuck shut.
•
CORRECTIVE ACTION: Disassemble pumps and open tubing or replace if
more than 6 months since last replaced.
•
SECTION: 8.6
•
POSSIBLE CAUSE: Waste line blocked.
•
CORRECTIVE ACTION: Clear tubing or replace.
•
SECTION: 8.6
•
POSSIBLE CAUSE: Waste line check valve stuck open.
•
CORRECTIVE ACTION: Flush check valve or replace.
•
POSSIBLE CAUSE: Bottle vent plugged, causing positive pressure in the waste
bottle.
•
CORRECTIVE ACTION: Loosen waste bottle cap to verify, clear blockage or
replace vent.
•
POSSIBLE CAUSE: Capillary tube plug broken loose from tube and stuck in
waste chamber port.
•
CORRECTIVE ACTION: Disassemble chamber, check for blockage in outlet
port (upper).
•
SECTION: 8.3
•
POSSIBLE CAUSE: Sample chamber outlet blocked.
•
CORRECTIVE ACTION: Disassemble chamber, check for blockage in outlet
tube due to salt buildup, and clean if necessary.
•
SECTION: 8.3
Stir bar fails to spin.
9-12
•
POSSIBLE CAUSE: Stir bar stuck to chamber.
•
CORRECTIVE ACTION: Clean the sample chamber.
•
SECTION: 8.3
•
POSSIBLE CAUSE: Stir motor failure.
•
CORRECTIVE ACTION: Go to stir motor diagnostics and verify.
•
SECTION: 7.4
9-13
9-14
10. Appendix A–Typical Performance Data
Testing of the YSI Model 1500 SPORT vs. the YSI Model 2300 STAT suggests
the following typical performance:
Capillary Tube Injector, YSI Model 1502
YSI 1500 vs 2300
WHOLE BLOOD - CAPILLARY / 0.25% TX-100 - LACTATE
30.0
28.0
26.0
24.0
22.0
1500 SPORT
( mmol/l )
20.0
18.0
16.0
14.0
12.0
10.0
8.0
6.0
4.0
2.0
0.0
2.0
6.0
10.0
14.0
18.0
22.0
26.0
30.0
YSI 2300 ( mmol/l )
Y = 0.9973X - .0705
DATA
Total Blood Lactate - YSI 1515 cell lysing agent added to the buffer solution in
both instruments.
Concentration
Pooled Est. Std. Dev.
Mean Bias
0-10 mmol/L
10-20
20-30
0.1 mmol/L
0.2
0.4
0.0 mmol/L
0.1
0.0
1010-1
YSI 1500 vs 2300
WHOLE BLOOD - CAPILLARY / NO TX-100 - LACTATE
30.0
28.0
26.0
24.0
22.0
1500 SPORT
( mmol/l )
20.0
18.0
16.0
14.0
12.0
10.0
8.0
6.0
4.0
2.0
0.0
2.0
6.0
10.0
14.0
18.0
22.0
YSI 2300 ( mmol/l )
Y = 1.0095X - .0224
DATA
Whole Blood Lactate - Extracellular ONLY
1010-2
Concentration
Pooled Est. Std. Dev.
Mean Bias
0-10 mmol/L
10-20
20-30
0.1 mmol/L
0.3
0.4
0.1 mmol/L
0.2
0.3
26.0
30.0
Blunt Needle Syringepet, YSI Model 1501
YSI 1500 vs 2300
WHOLE BLOOD - SYRINGEPET / 0.25% TX-100 - LACTATE
30.0
28.0
26.0
24.0
22.0
1500 SPORT
(mmol/l )
20.0
18.0
16.0
14.0
12.0
10.0
8.0
6.0
4.0
2.0
0.0
2.0
6.0
10.0
14.0
18.0
22.0
26.0
30.0
YSI 2300 ( mmol/l )
Y = 1.0078X - .0714
DATA
Total Blood Lactate - YSI 1515 cell lysing agent added to the buffer solution in
both instruments.
Concentration
Pooled Est. Std. Dev.
Mean Bias
0-10 mmol/L
10-20
20-30
0.1 mmol/L
0.3
0.5
0.1 mmol/L
0.3
0.2
1010-3
YSI 1500 vs 2300
WHOLE BLOOD - SYRINGEPET / NO TX-100 - LACTATE
30.0
28.0
26.0
24.0
22.0
1500 SPORT
( mmol/l )
20.0
18.0
16.0
14.0
12.0
10.0
8.0
6.0
4.0
2.0
0.0
2.0
6.0
10.0
14.0
18.0
22.0
26.0
YSI 2300 ( mmol/l )
Y = 1.0076X - .1302
DATA
Whole Blood Lactate - Extracellular ONLY
1010-4
Concentration
Pooled Est. Std. Dev.
Mean Bias
0-10 mmol/L
10-20
20-30
0.1 mmol/L
0.3
0.4
0.1 mmol/L
0.2
0.3
30.0
YSI 1500 vs 2300
PLASMA - SYRINGEPET / 0.25% TX-100 - LACTATE
30.0
28.0
26.0
24.0
22.0
1500 SPORT
( mmol/l )
20.0
18.0
16.0
14.0
12.0
10.0
8.0
6.0
4.0
2.0
0.0
2.0
6.0
10.0
14.0
18.0
22.0
26.0
30.0
YSI 2300 ( mmol/l )
Y = 0.9793X - .1345
DATA
Plasma Lactate - YSI 1515 cell lysing agent added to the buffer solution in both
instruments.
Concentration
Pooled Est. Std. Dev.
Mean Bias
0-10 mmol/L
10-20
20-30
0.0 mmol/L
0.1
0.4
0.0 mmol/L
0.1
0.4
1010-5
YSI 1500 vs 2300
PLASMA - SYRINGEPET / NO TX-100 - LACTATE
30.0
28.0
26.0
24.0
22.0
1500 SPORT
(mmol/l)
20.0
18.0
16.0
14.0
12.0
10.0
8.0
6.0
4.0
2.0
0.0
2.0
6.0
10.0
14.0
18.0
22.0
26.0
30.0
YSI 2300 ( mmol/l )
Y = 0.9893X - .1559
DATA
Plasma Lactate - Without YSI 1515 cell lysing agent added to the buffer solution
of either instrument.
1010-6
Concentration
Concen tration
Pooled Est. Std. Dev.
Mean Bias
0-10 mmol/L
10-20
20-30
0.1 mmol/L
0.2
0.3
0.1 mmol/L
0.1
0.1
11. Appendix B–Sample Collection and Handling
The YSI 1500 SPORT is not intended for home use. Proper sample collection
and preparation, by a professional, are vital factors in obtaining reliable results
with the YSI SPORT. This section covers the basic guidelines for collecting and
preparing whole blood, plasma and serum samples.
Much of the Blood Plasma and Blood Serum material is abstracted from
“Workshop Manual of Methods for the Determination of Glucose” by Gerald R.
Cooper, M.D., Ph.D. published by the Commission on Continuing Education,
Council on Clinical Chemistry, American Society of Clinical Pathologists
(1966).
Blood Plasma
Be sure that all collection equipment is sterile and chemically clean. Use aseptic
techniques in handling the sample from collection through assay.
During or immediately after collection, mix the specimen with an anticoagulant
and preservative to prevent coagulation, inhibit enzyme activity and prevent
bacterial growth. Separate the red blood cells by centrifugation within 30
minutes of collection. If delays between collection and assay are unavoidable,
follow one of these procedures:
1. Refrigerate the whole blood at +4°C. This is acceptable for periods up to
four hours.
2. Or, refrigerate the plasma with preservatives at +4°C for up to three days.
Plasma with preservative left at room temperature may keep up to four
hours.
3. Or, freeze the cell-free plasma with preservative at -10°C or colder. Plasma
will keep indefinitely at this temperature. Thaw the frozen plasma rapidly at
25°C and analyze promptly. The freeze-thaw cycle should not be repeated.
Tubes containing cells should never be frozen.
NOTE: Chilling or freezing may precipitate aggluten or fibrinogen masses
which may not redissolve. In such cases, remove them by centrifugation before
assay.
Several common anticoagulants are suitable for use with specimens being
prepared for assay. These include:
Potassium oxalate in a concentration of 2 mg/ml of blood.
Sodium fluoride in a concentration of 5-10 mg/ml of blood.
EDTA in a concentration of 1 mg/ml of blood.
1111-1
Sodium fluoride and oxalate in combination in a total concentration of 2
mg/ml of blood.
Heparin sodium as commonly supplied in Vacutainer collection tubes.
WARNING: The YSI SPORT is not suitable for use with
samples containing thymol or other phenol preservatives.
These are interfering substances.
Oxalate anticoagulants, and particularly the combination of sodium fluoride and
oxalate, may produce hemolysis, which can cause disagreement between results
from the SPORT and some reference methods.
Warning: Frozen blood-bank blood, which contains a high
percentage of glycerine, is not a suitable specimen for
diagnostic work with the SPORT.
Blood Serum
Draw blood for serum preparation into a dry tube without additives. Siliconecoated tubes and stoppers lubricated with glycerine or silicone are acceptable.
After the specimen stands 15 minutes at room temperature, loosen the clot from
the wall of the tube with a sterile glass rod or applicator. Add sterile beads, if
desired. Centrifuge the specimen promptly to separate the serum from the clot.
If delays are unavoidable follow one these procedures:
1. Refrigerate the whole blood at +4°C. This is acceptable for periods of up to
4 hours.
2. Or, prolonged storage, isolate the serum from the clot within 30 minutes of
collection and freeze it at -10°C or colder. Serum will keep indefinitely at
this temperature. Thaw the frozen serum rapidly at 25°C and analyze
promptly. The freeze-thaw cycle should not be repeated. Tubes containing
cells should never be frozen.
NOTE: Freezing may precipitate aggluten or fibrinogen masses which may
not redissolve. You can remove these by centrifugation before assay.
3. Or by adding a chemical preservative. Preservatives are not commonly used
with serum; however, sodium fluoride in concentrations of 7.5 mg/ml of
blood, EDTA in concentrations of 1 mg/ml of blood, or boric acid in
concentrations of 8 mg/ml of blood will substantially halt glycolysis in
most, but not all, specimens. If sodium iodoacetate is used as an
antiglycolytic, it must be free of iodide and iodine.
1111-2
WARNING: The YSI Model 1500 is not suitable for use with
samples containing thymol or other phenol preservatives.
These are interfering substances.
Warning: Frozen blood-bank blood, which contains a high
percentage of glycerine, is not a suitable specimen for
diagnostic work with the Model 1500.
Whole Blood
If more than five minutes will elapse between the time you draw a specimen and
analyze it, you should mix the blood with an anticoagulant during or
immediately after collection. Analysis of whole blood without an anticoagulant
is not recommended. The preferred anticoagulants are EDTA in a concentration
of 1 mg/ml of blood, or heparin sodium as commonly supplied in Vacutainer
collection tubes.
Other effective anticoagulants which may be used are potassium oxalate in a
concentration of 2 mg/ml of blood, sodium fluoride in a concentration of 5-10
mg/ml of blood, or sodium fluoride and EDTA in a concentration of 2.5mg
fluoride and 1 mg EDTA per ml of blood. These are not know to cause any error
in the measurement of whole blood lactate, but they may cause a reduction of
the sample's hematocrit value with or without accompanying hemolysis. This
would lead to a slight error in the calculated plasma lactate value. Hemolysis
can cause disagreement between readings on the Model 1500 and some
reference methods.
Cell metabolism will result in increased lactate concentrations in the specimen
through glycolysis, which will result in erroneously high assay values.
Glycolysis is particularly rapid in whole blood specimens, and these should be
analyzed within an hour of drawing. Refrigeration at +4°C will retard glycolysis
and extend the acceptable delay to four hours. Whole blood specimens must not
be frozen. Some authorities recommend the use of chemical preservatives to
minimize glycolysis:
Fluoride in concentrations of 7.5 mg/ml of blood.
Boric acid in concentrations of 8 mg/ml of blood.
Sodium iodoacetate; if sodium iodoacetate is used as the antiglycolytic, it
must be free of iodide and iodine.
YSI has not evaluated the effects of these antiglycolytics.
1111-3
WARNING: The YSI SPORT is not suitable for use with
samples containing thymol or other phenol preservatives.
These are interfering substances.
Before taking a sample from a whole blood specimen, any cells which may have
settled out must be resuspended. Invert the collection tube three times, gently
enough not to cause frothing, just before sampling. Samples which have settled
for more than an hour (particularly if they have been refrigerated) should be
placed on a blood rotator for five minutes to break up clumps of cells.
Air bubbles or froth in the sample drawn into the sipper can lead to significant
errors in the form of low lactate readings. Because it is difficult to detect
bubbles visually in whole blood specimens, you should exercise great care. Any
unexpectedly low lactate readings may indicate a bubble; if possible, repeat the
determination.
Warning: Frozen blood-bank blood, which contains a high
percentage of glycerine, is not a suitable specimen for
diagnostic work with the SPORT.
1111-4
12. Appendix C–Cell Lysing
Obtaining reliable results that have value requires a basic understanding of what
is going on inside the whole blood sample, at least as it relates to lactate.
Lactate is always present in the blood plasma at some level due to minor
anaerobic activity. As the consumption of free oxygen increases during exercise,
anaerobic activity increases, and so does the lactate concentration.
Lactate production starts inside the muscle cell, and the lactate then diffuses
through the cell wall into the blood cell carrier fluid, plasma. The plasma lactate
will then in turn diffuse through the red blood cell walls in order to achieve an
equilibrated state. It takes several minutes for this to happen. As a result,
depending at which point in an exercise the whole blood sample is taken, the
concentration of plasma lactate (extracellular), may be significantly different
than the concentration of lactate in the red blood cell (intracellular).
For example if the sampling was done very early, the equilibration of the plasma
lactate and the cell lactate will not have taken place yet and the plasma lactate
concentration will be higher. Conversely, if the sampling was done much later,
the lactate concentrations will be much closer or equal.
In a nonnon - lysing system the blood cell walls create a barrier that the intracellular
lactate can "hide behind". When a blood sample is injected into the sample
chamber, the extracellular lactate dilutes into the buffer solution immediately.
The intracellular lactate does not however due to the time required to diffuse
through the cell walls. Given sufficient time the lactate would diffuse through
the cell walls and then dilute into the buffer solution allowing all of the the
lactate to be measured. This is not practical however as the rate at which this
diffusion would take place would severely lengthen the duration of each
measurement cycle. Therefore the only lactate that can be measured on a
SPORT in a nonnon- lysing system is extracellular.
What this means quite simply is that without a way of assuring that the cell
walls have been removed when we do a sample measurement, we can never
expect to get a true TOTAL LACTATE reading on the SPORT
This can be done by introducing certain chemicals into the buffer solution inside
the sample chamber that effectively tear apart the cell walls of the blood sample
immediately upon contact. This allows both the intracellular and extracelleular
lactate to dilute immediately into the buffer solution, and because preservatives
now can access the contents of the cell, any additional lactate production is
prevented within the sample.
Another variable that plays a significant role in the lactate equation is how the
sample is cared for prior to measurement. If the sample is exposed to elevated
temperatures before measurement it is quite likely that additional lactate
production will continue within the unlysed blood cells. This can add to the
1212-1
inaccuracy of determining an athletes actual lactate level, which can affect the
appraisal of the athletes performance.
IMPORTANT: YSI strongly recommends that you use the YSI 1515 Cell
Lysing Agent. Without total cell lysis (breaking apart of cell walls), results
obtained on whole blood can vary significantly depending on individual
hematocrit levels, on whether unintended partial cell lysis takes place, on
temperature changes, and the amount of time that elapses from the time of
sample collection until the analysis is completed.
The presence of the lysing agent in the buffer solutions eliminates these
problems, and also allows the results to be compared directly to more traditional
total blood lactate laboratory techniques.
Using the YSI 1515 Cell Lysing Agent
If your sample measuring protocol requires the total lysis of blood cells, or if
you are going to preserve the samples with the YSI 2315 Preservative Tube Kit
for later analysis, another step is required in the reagent preparation process
explained in Section 3.3 Reagent Preparation.
Buffer Solution
Fill the 500mL mixing bottle with reagent water. Reagent water must be very
pure. Use high quality deionized water or distilled water. Add one package of
YSI 2357 Buffer Concentrate and stir, waiting long enough to be certain that the
buffer chemicals have completely dissolved.
Add one packet of YSI 1515 Cell Lysing Agent and stir.
Pour the solution into the instrument Buffer Bottle.
Reference Solution
Fill the 500mL mixing bottle with reagent water. Reagent water must be very
pure. Use high quality deionized water or distilled water. Add one package of
YSI 2357 Buffer Concentrate and stir, waiting long enough to be certain that the
buffer chemicals have completely dissolved.
Add 4.5 mL of YSI 1530 30 mmol/L Calibration Standard, and stir as necessary.
This should be measured out with the graduated syringe supplied in the 1504
Starter Kit. Replace the bottle lid and shake the bottle for 15 seconds to insure
complete mixing.. It is important for reliable results that the reference
solution is completely mixed.
Add one packet of YSI 1515 Cell Lysing Agent and stir.
Pour this solution into the Reference Bottle.
1212-2
WARNING: If you switch from running with, to running
without the lysing agent, or vice versa, you cannot get
reliable results unless you allow sufficient time for
complete removal of the agent from the fluid system.
1212-3
1212-4
13. Appendix D Sample Preservation
It is possible to collect samples for later analysis if the situation dictates it. For
this application the YSI 2315 Preservative Tube Kit is available. The kit
consists of re-sealable tubes containing a preservative powder and packets of
YSI 1515 Cell Lysing Agent.
WARNING: You must use the YSI Cell Lysing Agent in your
SPORT when running samples preserved in YSI
preservative tubes.
You cannot use the YSI preservative tubes unless the lysing agent is used in the
instrument when the actual measurement takes place. This is due to the fact that
the preservative tubes contain a detergent. This detergent, when introduced into
a system without the cell lysing agent, can affect the membrane response and
cause incorrect readings. While this detergent is also present in the cell lysing
agent, the response effect is compensated for during normal calibration, and the
sudden introduction of a sample containing the detergent will have no adverse
effect. Refer to Appendix E–Effects Of Selected Substances.
A sample size of 100 microliters minimum is required for each tube.
Samples preserved in YSI preservative tubes can be stored for up to 48 hours @
25°C, and for up to 7 days @ 5°C.
1313-1
1313-2
14. Appendix E–Effects Of Selected Substances
Paracetamol (Tylenol, Acetaminophen) can be a significant interference in
overdose situations.Effective therapeutic levels of Paracetamol in blood (0.07 0.14 mmol/L) are not a problem. The pharmacokinetic literature suggests that
one would have to injest more than 3 times the recommended therapeutic dose
in order for the plasma levels to be greater than 0.17mmol/L.
Paracetamol interferees with the SPORT measurement because it can penetrate
the cellulose acetate portion of the enzyme membrane and is oxidizable at the
platinum eletrode at the applied polarization voltage. The presence of elevated
levels of Paracetamol in a sample results not only in an elevated lactate reading,
but also a ramping signal in the time region in which a steady state signal is
normally observed.
Warning: The following preservatives interfere with the
measurement and should not be used: Phenol,
Benzalkonium Chloride, Methyl Paraben, Per chloric Acid,
Sodium Azide, Thymol, Trichloracetic Acid.
Several classes of chemicals can damage the YSI sensor system or cause
erroneous readings. Most of these have no place in diagnostic medicines, but
may be of scientific interest. Some substances such as triglycerides, which are
interferences for photometric sensor systems, do not interfere with the SPORT
electrochemical sensor system.
Reducing Agents
Many reducing agents would give rise to a false signal current (and falsely
elevated reading) if they succeeded in reaching the sensing anode of the YSI
probe. Most of these are excluded from the probe by the cellulose acetate layers
of the membrane, However, thymol, phenols, anilines, hydra zines and
hydrazides, hydroxylamines, oximes and a few other compounds of molecular
weight below 150 which are cationic or uncharged in neutral solution can
interfere.
Homologues and isomers may be expected to behave similarly, except that
relative response generally declines with increasing molecular (or ionic)
bulkiness. Hydrogen Sulfide, Hydrazine, Methylhydrazine, Phenylhydrazine,
Oxamic Hydrazide, Hydroxyet hylhydrazine, Acetone Oxime, Hydroxylamine
and Sodium Borohydride are also known to give significant relative response.
Relative response to reducing agents may vary from membrane lot to membrane
lot, and may depend on the service history of the probe and membrane.
1414-1
The following reducing agents may result in an elevated background current
when present at low levels (only a few milligrams per liter):
Aniline
Phenol
Thiocarboyhdrazide
Thiourea
Resorcinol
Guaiacol
2-Mercaptoethanol
Ethyl Carbazate
Formic Acid Hydrazide
Catechol
Acetaminophen
The following materials may cause trouble at higher concentrations:
2-Amino-4-nitrophenol
Hydroquinone
p-Cresol
p-Phenylenediame
Isoniazid
o-Toluidine
Methimazole
2-Hydroxybenzyl Alcohol
4-Ethylphenol
p-Aminophenol
Pyrogallol
Oxalyl Dihydrazide
N.N-Dimethylhydroxylamine
Sodium Azide
Calibration Shift by Detergents
The sensitivity of the platinum anode of the sensor is affected somewhat by
absorption or desorption of material from the buffer which bathes it. In normal
operation, such changes are quite gradual and are corrected in the periodic
recalibration of the instrument. However, concentrated detergent solutions may
have a much more sudden effect. Concentrated anionic detergents can cause
readings to be abruptly elevated or depressed; concentrated nonionic detergents
generally depress readings. For this reason, concentrated detergent solutions
should not be sampled. No problem has been observed from the naturally
occurring detergency of specimens.
1414-2
Endogenous and Exogenous Substances
YSI has tested hundreds of substances to determine whether they have any effect
on the sensor system used in the SPORT. YSI has also verified that the addition
of Triton X-100 to the SPORT instrument buffer does not significantly alter the
extent to which these materials interfere with the instrument.
The endogenous substances listed were all tested at levels far higher than can be
found in the body, and all were found to be noninterfering at the highest
naturally occurring levels. The column headed "Interfering Level" indicates the
concentration at which each substance might be expected to cause an error of 1
mmol/l in the lactate reading. Certain exogenous substances can interfere with
measurements, and nothing should be added to the specimens except those
anticoagulants and antiglycolytics recommended in Appendix B–Sample
Collection and Handling.
Recent information indicates that some of the exogenous substances listed are
now drugs that are abused, that is, ingested at levels much higher than the
usually recommended therapeutic doses. In patients with higher than therapeutic
levels, there is hazard of gross masking of lactate concentration by the
interfering substance. The SPORT should not be used to analyze specimens
containing any of these substances at or above the listed Interfering Level.
Physical Damage
Never inject concentrated mineral acids, concentrated bases, or strong organic
solvents into the YSI SPORT as these may permanently damage the enzyme
membranes or the plastic parts of the probe and sample chamber. Do not inject
water-insoluble oils or greases, because it may be difficult to clear them from
the sample chamber. Blood specimens with excessive content of fats are not a
problem.
Substance
Weight
Lactate Interfering Level
mg/dL
mmol/L
Anticoagulants:
Sodium Oxalate
Heparin Sodium
Dipotassium EDTA
Sodium Citrate
134.00
-404.46
294.10
-- --- --- --- --
Preservatives:
Sodium Flouride
Iodoacetic Acid
2-Iodoacetamide
Sodium Tetraborate 10 H2O
Benzalkonium Chloride
Cetylpyridinium Chloride
Sodium Azide
Methylparaben
Phenol
Thymol
42.00
185.96
185.95
381.37
396.11
357.99
65.01
152.15
94.11
150.22
-- -24,346
3,356
54,000
1,200
-- -588
168
6.7
600
1309
180
1416
30
90
11
0.71
40
1414-3
1414-4
Substance
Weight
Lactate Interfering Level
mg/dL
mmol/L
Lactic Acid Analogues
DL-a-Glycerophosphate,Disodium
DL-Glyceric Acid, hemi-calcium
Glycolic Acid
B-Hydroxypyruvic Acid,Lithium salt
DL-B-Hydroxybutyric Acid,Sodium salt
4-Hydroxybutyric Acid,Sodium salt
DL-Malic Acid
DL-a-Hydroxybutyric Acid
L-B-Phenyllactic Acid
B-Chlorolactic Acid
Oxalacetic Acid
Glycine
DL-Alanine
B-Alanine
DL-a-Hydroxycaproic Acid
Pyruvic Acid,Sodium Salt
DL-a-Hydroxyisovaleric Acid
L-a-Hydroxyisocaprioc Acid
a-Hydroxyisobutyric Acid
Glycerol
216.4
286.25
76.05
110
126
126.09
134.09
126.09
166.18
124.52
132.07
75.07
89.09
89.09
132.16
110.04
118.13
132.16
104.11
92.09
-- -1276
177
-- --- --- --- -84
24
93
-- --- --- --- -45
-- --- -75
-- --- --
9.6
23
6.7
1.4
2.4
3.4
5.7
Substance
Weight
Lactate Interfering Level
mg/dL
mmol/L
Drugs, Poisons, and Miscellaneous
Exogenous Substances:
Acetaminophen
Metaphosphoric Acid
Ethanol
Methanol
Iso-Propanol
Ethylene Glycol
Acetylsalicylic Acid
Formaldehyde
Hydrogen Peroxide
D-Penicillamine
Salicylamide
Sodium Nitrite
Sodium Salicylate
Ascorbic Acid
D-Galactose
D-Glucose
D(+)Glucosamine HC1
Glucose-6-phosphate, Monosodium
Uric Acid
p-Aminosalicylic Acid
2,3-Dimercapto-propanol
Guaiacol
Hydrazine Sulfate
Hydroquinone
Hydroxylamine Hydrochloride
Isoniazid
2-Mercaptoethanol
p-Phenylenediamine HC1
Potassium Cyanide
Potassium Iodide
Potassium Thiocyanate
Pyridoxine Hydro-chloride
Sodium Sulfide Nonhydrate
2-Thiouracil
Thiourea
o-Tolidine Dihydro-chloride
o-Toluidine
151.17
-46.07
32.04
60.10
62.07
180.16
30.03
34.02
149.21
137.14
69.00
160.11
176.12
180.16
180.16
215.64
282.12
168.11
153.14
124.22
124.14
130.12
110.11
69.49
137.14
78.13
181.07
65.12
166.01
97.18
205.64
240.18
128.15
76.12
285.22
107.16
15
-- --- --- --- --- --- -2606
1.1
4376
12
108
-- -1800
-- --- --- --- --- -8.0
-- -3.1
13
4.3
1.6
12
1.0
2.9
-- -209
107
192
0.9
-- -3.2
-- -3.60
1.0
868
0.32
293
0.88
15
102
0.52
0.25
1.0
0.39
0.23
0.88
0.13
0.165
12
11
9.3
0.04
0.42
.34
1414-5
1414-6
15. Appendix F–Temperature Effect on Operation
Both the measurement chemistry and the electrical performance have very
predictable reactions to changes in ambient temperatures. To correct for these
changes, a temperature compensation algorithm has been included in the result
calculation; however, to guarantee accurate results we compensate only over a
limited range. It may be possible that in some situations the 1500 SPORT may
appear to be overly sensitive to "TEMPERATURE STABILITY ERRORS". If
that is the case, there are simple precautions that should be taken to insure the
quality of the results.
Whenever possible, the 1500 SPORT and the fluid supplies should be
equilibrated to the ambient temperature of the actual testing site and then
calibrated. This is quite easily done when testing indoors but requires special
effort if done outdoors.
When testing outdoors the 1500 SPORT should be kept out of direct sunlight.
Direct sunlight can cause rapid warming of the sample chamber, warming the
contents much faster than the larger fluid supply bottles. If this happens it is
quite possible that the 1500 SPORT will detect "TEMPERATURE STABILITY
ERRORS". Simply keeping the unit in the shade regardless of ambient
temperature will assure that the warming or cooling effects are consistent, and
therefore the results accurate.
Temperature Effect On Battery Life
The length of available sampling time is affected by the ambient temperature
while testing. The colder the temperature, the more battery life is affected.
Typically the results of our testing indicate the following estimated guidelines:
Ambient Temperature
Sampling Duration
Samples Ran
45°C
25°C
5°C
8 Hours
8 Hours
7 Hours
90
90
75
IMPORTANT:
1. All testing done on units that were precharged for 8 hours using YSI Model
1550 wall mounted chargers.
2. These tests were conducted running samples continuously (1 every 2
minutes). It is quite clear that if fewer samples are being run, the actual
sampling duration will increase.
3. Sampling duration is defined as starting point to software lockout. Refer to
Section 5.8 Software Structure, Battery Level Checks for more information
relating to battery voltage monitoring.
1515-1
1515-2
16. Appendix G–Accessory Products
The following products are available from YSI or your sales representative.
YSI Catalog
Number
1501
1502
1503
1504
1505
1506
1515
1530
015117
015118
2315
2327
2328
2329
2357
2363
2392
2751
Description
25 microliter, Blunt Needle Syringepet
25 microliter, Capillary Tube Injection Device
1500 Preventative Maintenance Kit
1500 L-Lactate Starter Kit
2327 Lactate Standard, 5 mmol/L
1530 Lactate Standard, 30 mmol/L
2329 Lactate Membranes
2363 Potassium Ferrocyanide
2392 NaCl Solution
2357 Buffer Concentrate Kit
1505 Capillary Tubes
1515 Cell Lysing Agent
Syringe, 3cc
25 microliter, Capillary Tube Package, (100 count)
Hewlet Packard Infrared Printer (HP 82240)
Cell Lysing Agent, (8 packs)
Lactate Standard, 30 mmol/L, (125mL)
120 VAC 60 Hz Battery Charger
220 VAC 50 Hz Battery Charger
Preservative Tube Kit
1515 Cell Lysing Agent
Preservative Tube
Lactate Standard, 5 mmol/L, (125mL)
Lactate Standard, 15 mmol/L, (125mL)
Lactate Membranes, (4 count)
Buffer Concentrate Kit, (8 packs)
Potassium Ferrocyanide, (125mL)
NaCl Solution, (30mL)
Printer Paper, (5 rolls/box)
Qty.
1
1
1
1
1
1
1
1
1
Qty.
1
50
1616-1
1616-2
17. Appendix H–Cleaning, Disinfecting and
Decontamination
Proper precautionary lab practices should be followed when handling biological
hazards.
Suggested cleaning and disinfecting solutions:
•
Isopropanol 70%
•
Sodium hypochlorite, 5000 ppm free available chlorine, (1:10 solution of
house hold bleach).
Disassemble parts that require cleaning, per instructions in Section 8
Maintenance. Thoroughly clean with the disinfecting agent. Rinse with warm
water.
WARNING:
WARN ING: Clean probes with isopropanol only and rinse with warm water.
Clean up all spills then reassemble.
1717-1
1717-2
18. Appendix I–Warranty and Shipping
Information
The YSI Model 1500 SPORT Analyzer is warranted for one year from date of purchase
by the end user against defects in materials and workmanship, exclusive of batteries.
Within the warranty period, YSI will repair or replace, at its sole discretion, free of
charge, any product that YSI determines to be covered by this warranty.
To exercise this warranty, write or call your local YSI representative, or contact YSI
Customer Service in Yellow Springs, Ohio. Send the product and proof of purchase,
transportation prepaid, to the Authorized Service Center selected by YSI. Repair or
replacement will be made and the product returned, transportation prepaid. Repaired or
replaced products are warranted for the balance of the original warranty period, or at
least 90 days from date of repair or replacement.
Limitation of Warranty
This Warranty does not apply to any YSI product damage or failure caused by (i)
failure to install, operate or use the product in accordance with YSI’s written
instructions, (ii) abuse or misuse of the product, (iii) failure to maintain the product in
accordance with YSI’s written instructions or standard industry procedure, (iv) any
improper repairs to the product, (v) use by you of defective or improper components or
parts in servicing or repairing the product, or (vi) modification of the product in any
way not expressly authorized by YSI.
THIS WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES, EXPRESSED OR
IMPLIED, INCLUDING ANY WARRANTY OF MERCHANTABILITY OR FITNESS FOR A
PARTICULAR PURPOSE. YSI’s LIABILITY UNDER THIS WARRANTY IS LIMITED TO
REPAIR OR REPLACEMENT OF THE PRODUCT, AND THIS SHALL BE YOUR SOLE
AND EXCLUSIVE REMEDY FOR ANY DEFECTIVE PRODUCT COVERED BY THIS
WARRANTY. IN NO EVENT SHALL YSI BE LIABLE FOR ANY SPECIAL, INDIRECT,
INCIDENTAL OR CONSEQUENTIAL DAMAGES RESULTING FROM ANY DEFECTIVE
PRODUCT COVERED BY THIS WARRANTY.
YSI Factory Service
Servi ce Centers
United States
YSI Incorporated • Repair Center • 1725 Brannum Lane • Yellow Springs, OH • 45387 • USA
Phone: 937 767-7241 • Fax: 937 767-9353
Europe
Analytical Technologies • Lynchford House • Lynchford Lane • Farnborough, Hampshire • GU14 GLT • England
Phone: 441 252 514711 • Fax: 441 252 511855
YSI Authorized Service Centers
California
Fisher Scientific ISD • 2822 Walnut Avenue, Suite E • Tustin, CA • 92681 • Phone: 800 395-5442
Georgia
Fisher Scientific ISD • 2775 Horizon Ridge Court • Suwanee, GA • 30174 • Phone: 800 395-5442
Illinois
Fisher • 1600 West Gleenlake Avenue • Itasca, Ill • 60143 • Phone: 800 395-5442
New Jersey
Fisher Scientific ISD • 52 Fadem Road • Springfield, NJ • 07081 • Phone: 800 395-5442
1818-1
Pennsylvania
Fisher Scientific ISD • 585 Alpa Drive • Pittsburgh, PA • 15238 • Phone: 800 395-5442
Cleaning Instructions
IMPORTANT: Before they can be serviced, equipment exposed to
biological, radioactive, or toxic materials must be cleaned and disinfected.
Biological contamination is presumed for any instrument, probe, or other device
that has been used with body fluids or tissues, or with wastewater. Radioactive
contamination is presumed for any instrument, probe or other device that has
been used near any radioactive source.
If an instrument, probe, or other part is returned or presented for service without
a Cleaning Certificate, and if in our opinion it represents a potential biological
or radioactive hazard, our service personnel reserve the right to withhold service
until appropriate cleaning, decontamination, and certification has been
completed. We will contact the sender for instructions as to the disposition of
the equipment. Disposition costs will be the responsibility of the sender.
When service is required, either at the user's facility or at YSI, the following
steps must be taken to insure the safety of our service personnel.
1. In a manner appropriate to each device, decontaminate all exposed surfaces,
including any containers. 70% isopropyl alcohol or a solution of ¼ cup
bleach to 1 gallon tap water are suitable for most disinfecting. Instruments
used with wastewater may be disinfected with 0.5% Lysol if this is more
convenient to the user.
2. The user shall take normal precautions to prevent radioactive contamination
and must use appropriate decontamination procedures should exposure
occur.
3. If exposure has occurred, the customer must certify that decontamination has
been accomplished and that no radioactivity is detectable by survey
equipment.
4. Any product being returned to the YSI Repair Center, should be packed
securely to prevent damage.
5. Cleaning must be completed and certified on any product before returning it
to YSI.
1818-2
Packing Instructions
1. Clean and decontaminate items to insure the safety of the handler.
2. Complete and include the Cleaning Certificate.
3. Place the product in a plastic bag to keep out dirt and packing material.
4. Use a large carton, preferably the original, and surround the product
completely with packing material.
5. Insure for the replacement value of the product.
Cleaning Certificate
Organization
Department
Address
City
State
Country
Phone
Model No. of Device
Lot Number
Zip
Contaminant (if known)
Cleaning Agent(s) used
Radioactive Decontamination Certified?
(Answer only if there has been radioactive exposure)
Yes
No
Cleaning Certified By
Name
Date
1818-3
1818-4
19. Appendix J–Required Notice
The Federal Communications Commission defines this product as a computing
device and requires the following notice:
This equipment generates and uses radio frequency energy and if not installed
and used properly, may cause interference to radio and television reception. It
has been type tested and found to comply with the limits for a Class A or Class
B computing device in accordance with the specification in Subpart J of Part 15
of FCC Rules, which are designed to provide reasonable protection against such
interference in a residential installation. However, there is no guarantee that
interference will not occur in a particular installation. If this equipment does
cause interference to radio or television reception, which can be determined by
turning the equipment off and on, the user is encouraged to try to correct the
interference by one or more of the following measures:
• reorient the receiving antenna
• relocate the computer with respect to the receiver
• move the computer away from the receiver
• plug the computer into a different outlet so that the computer and
receiver are on different branch circuits.
If necessary, the user should consult the dealer or an experienced
radio/television technician for additional suggestions. The user may find the
following booklet prepared by the Federal Communications Commission
helpful: "How to Identify and Resolve Radio-TV Interference Problems." This
booklet is available from the U.S. Government Printing Office, Washington,
D.C. 20402, Stock No. 0004-000-00345-4.
1919-1
YSI Incorporated
1725 Brannum Lane
Yellow Springs, Ohio 45387 USA
937 767-7241 • 800 659-8895
Fax 937 767-8058
Info@ysi.com
www.YSI.com
CEPartner4U BV
Esdoornlaan 13
3951DB Maarn
The Netherlands
+31 (0) 6-516.536.26
015162
A15162H
2003-12
 2003 YSI Incorporated
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