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Agilent 8000
Dissolution Sampling
Station
Operator’s Manual
Agilent Technologies
Notices
© Agilent Technologies, Inc. 2014
No part of this manual may be reproduced in any form or by any means (including electronic storage and retrieval or translation into a foreign language) without prior agreement and written consent from Agilent Technologies, Inc. as governed by
United States and international copyright laws.
Manual Part Number
70-9060
Edition
Rev C, May 2014
Agilent Technologies, Inc.
3501 Stevens Creek Blvd.
Santa Clara, CA 95052 USA
Warranty
The material contained in this document is provided “as is,” and is subject to being changed, without notice, in future editions. Further, to the maximum extent permitted by applicable law, Agilent disclaims all warranties, either express or implied, with regard to this manual and any information contained herein, including but not limited to the implied warranties of merchantability and fitness for a particular purpose. Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing, use, or performance of this document or of any information contained herein.
Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms, the warranty terms in the separate agreement shall control.
Technology Licenses
The hardware and/or software described in this document are furnished under a license and may be used or copied only in accordance with the terms of such license.
Restricted Rights Legend
U.S. Government Restricted Rights. Software and technical data rights granted to the federal government include only those rights customarily provided to end user customers. Agilent provides this customary commercial license in Software and technical data pursuant to FAR 12.211
(Technical Data) and 12.212 (Computer
Software) and, for the Department of
Defense, DFARS 252.227-7015 (Technical Data - Commercial Items) and DFARS
227.7202-3 (Rights in Commercial Computer Software or Computer Software
Documentation).
Safety Notices
CAUTION
A CAUTION notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met.
WARNING
A WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in personal injury or death. Do not proceed beyond a
WARNING notice until the indicated conditions are fully understood and met.
8000 Operator’s Manual
Contents
8000 Operator’s Manual
Figures
Tables
9
1 Safety
11
12
13
14
2 Introduction
17
Conventions Used in this Manual 17
18
3 Setting Up the 8000
Unpacking of 8000 and Components 20
21
Unpacking / Setup of 8000 & Peristaltic Pump 22
Unpacking / Setup of 8000 & Syringe Pump
26
Unpacking / Setup of 8000, Syringe Pump, and Filter
29
Setup of 8000 and Components 34
Installing 8000 Full Flow Filters 34
34
36
3
Contents
4
Installing the Media Rinse Reservoir
36
Installing and Connecting the Autocalibration Block
37
38
Loading Filters (Filter Changer only)
40
Changing Filter Types (Filter Changer Only) 40
41
4 Operating the 8000
Operating the 8000 with Apparatus 1 / 2
46
46
8000 Menu Options - Apparatus 1 / 2
48
61
66
Operating the 8000 with Apparatus 3 / 7
72
72
8000 with Apparatus 3 / 7 - Menu Options 74
86
95
Volumetric Calibration (Peristaltic Pump only)
97
99
Checking the Calibration - Volume Accuracy
101
102
Printer Operation and Communications
103
5 Maintenance and Troubleshooting
105
Clean System with Peristaltic Pump 106
Clean System Function with Syringe Pump 111
8000 Operator’s Manual
8000 Operator’s Manual
Contents
Syringe and Seal Replacement 112
113
System Cleaning Procedure with Syringe Pump and Filter
115
Cleaning the Filter Changer Drip Tray
117
Cleaning the Media Replacement Pump
117
Report Center Impact Printer 119
Installing the Cartridge Ribbon
119
120
121
122
Obtaining Warranty and Other Services
123
Index
125
5
Contents
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6 8000 Operator’s Manual
Figures
Figure 1. Agilent 8000 Dissolution Sampling Station
21
22
Figure 3. Cable Connections for 8000, Peristaltic Pump, and VK
24
25
Figure 6. Cable Connections for 8000, Syringe Pump, and VK
27
Figure 7. Y Cable 5075-0446 and Adapter 5075-0852 28
30
Figure 9. Cable Connections for 8000, Syringe Pump, Filter
Changer, and VK 7000 / 7010 31
Figure 10. Y Cable 5075-0446 and Adapter 5075-0852 32
Figure 11. Resident Probe Housing and Sample Cannula
33
Figure 12. Internal Media Replacement Module
38
Figure 13. External Media Replacement Module 39
46
72
Figure 16. The Cleaning Process 108
8000 Operator’s Manual 7
Figures
This page was intentionally left blank, except for this message.
8 8000 Operator’s Manual
Tables
8000 Operator’s Manual
41
Table 2. Front Panel Options (Apparatus 1 / 2)
47
Table 3. Main Menu Options (Apparatus 1 / 2)
49
Table 4. Set Clock and Alarms Options (Apparatus 1 / 2) 51
Table 5. Disso Parameters Options (Apparatus 1 / 2) 53
Table 6. Set Syringe Pump Parameters (Apparatus 1 / 2) 55
Table 7. Disso Control/Monitor Options (Apparatus 1 / 2)
57
Table 8. Manual Operations Options (Apparatus 1 / 2) 58
Table 9. Operate Syringe/Filter Changer Options (Apparatus 1 /
59
Table 10. Program Variables Options (Apparatus 1 / 2)
62
Table 11. Select Start Mode Options (Apparatus 1 / 2)
68
Table 12. Disso Options (Apparatus 1 / 2) 69
Table 13. Select Disso Program Start Mode Options (Apparatus
71
Table 14. Front Panel Options (Apparatus 3 / 7)
73
Table 15. Main Menu Options (Apparatus 3 / 7)
75
Table 16. Set Clock and Alarms Options 77
Table 17. Set App 3/7 Parameters Options (Apparatus 3 /
79
Table 18. Set Syringe Pump Parameters Options (Apparatus 3 /
81
Table 19. App 3/7 Control/Monitor Options
83
Table 20. Manual Operations (Apparatus 3 / 7)
84
Table 21. Operate Syringe/Filter Changer Options 85
Table 22. Program Variables Options (Apparatus 3 / 7)
88
Table 23. Select Start Mode Options (Apparatus 3 / 7) 93
94
9
Tables
Table 25. Manual Sampling Options
95
Table 26. Volume Accuracy Parameter Options 101
Table 27. Print Selections Options 104
Table 28. Program Auto Clean Variables
110
115
Table 30. Program Variables 116
10 8000 Operator’s Manual
Agilent 8000 Dissolution Sampling Station
Operator’s Manual
1
Safety
The Agilent 8000 has been designed and tested so that when used properly you have an accurate, fast, flexible, and safe instrument.
If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.
Operation of a Agilent 8000 involves the use of aqueous liquids and various pharmaceutical dosage forms. Unskilled, improper, or careless use of this instrument can create shock hazards, fire hazards, or other hazards which can cause death, serious injury to personnel, or severe damage to equipment and property.
Information on safety practices is provided with your instrument and operation manuals. Before using your instrument or accessories, you must thoroughly read these safety practices.
Observe all relevant safety practices at all times.
Agilent Technologies
11
1 Safety
Electrical Hazards
The 8000 contains electrical circuits, devices, and components operating at dangerous voltages. Contact with these circuits, devices, and components can cause death, serious injury, or painful electric shock.
Panels or covers that are retained by fasteners which require the use of a tool for removal may be opened only by Agilent-trained,
Agilent-qualified, or Agilent-authorized service engineers. Consult the manuals or product labels supplied with the 8020 to determine which parts are operator-accessible.
Application of the wrong supply voltage, connection of the instrument to an incorrectly wired supply outlet, or lack of proper electrical grounding can create a fire hazard or a potentially serious shock hazard and could seriously damage the instrument and any attached ancillary equipment.
Always use a three-wire outlet with ground connection which is adequately rated for the load. The installation must comply with local, state, and federal safety regulations.
Do not connect the instrument to the main power supply until you have made sure that the operating voltage is correctly set for the main power supply in the specific outlet in your laboratory to which the equipment will be connected.
12 8000 Operator’s Manual
Safety 1
Warning
WARNING
A ‘Warning’ message appears in the manual when failure to observe instructions or precautions could result in death or injury.
Read all warnings and cautions carefully and observe them at all times.
Caution
CAUTION
A ‘Caution’ message appears in the manual when failure to observe instructions could result in damage to equipment (Agilent supplied and / or other associated equipment).
Note
NOTE
A ‘Note’ appears in the manual to give advice or information.
8000 Operator’s Manual 13
1 Safety
14
Information Symbols
I
0
Switches main power on
Switches main power off
Indicates single-phase alternating current
Indicates the product complies with the requirements of one or more European Union
(EU) directives.
Indicates specific equipment meets standards of safety. These products are safe for use in the workplace for North America.
Indicates that this product must not be disposed of as unsorted municipal waste.
All Agilent products that are subject to the
WEEE directive shipped after August 13, 2005 are compliant with the WEEE marking requirements. Such products are marked with the
“crossed out wheelie bin” WEEE symbol in accordance with European Standard EN 50419.
For more information on collection, reuse, and recycling systems, please contact your local/regional waste administration, your local distributor, or Agilent.
Indicates the product complies with regulatory compliance requirements of New Zealand and
Australia.
8000 Operator’s Manual
Agilent 8000 Dissolution Sampling Station
Operator’s Manual
2
Introduction
Conventions Used in this Manual 17
Agilent Technologies
15
2 Introduction
The 8000 Dissolution Sampling Station provides an unattended solution for automated sampling from a dissolution apparatus. The instrument may be configured to control any instrument setup with a USP apparatus
1 (Baskets), 2 (Paddles), 3 (Reciprocating Cylinder), 5 (Paddle over
Disk), 6 (Rotating Cylinder), or 7 (Reciprocating Holder) offered by
Agilent Technologies, Inc. The firmware can store up to 15 different sampling programs. Dissolution apparatus parameters are entered as part of the 8000 program. These parameters are downloaded to the dissolution apparatus when beginning a test with the 8000.
The 8000 fail-safe sample delivery uses either the 810 Peristaltic Pump or 806 Syringe Pump optionally coupled with the automated 808 Filter
Changer. Hard-copy documentation is provided by the built-in Report
Center Printer which prints all essential parameters for proof of proper operation and compliance with current GMPs.
The 8000 may be configured with a replacement media (RM) module which replenishes any sample volume removed with fresh media.
Sample is delivered into test tubes or pre-capped HPLC autosampler vials for direct transfer of samples to an HPLC system. Sample trays are available in a variety of sizes to accommodate various sizes of vials or test tubes.
The 8000 is automation ready. An RS232 port permits integration with other automated systems. Sampling runs can be activated with an external switch closure or TTL signal. The 8000 has a programmable
TTL output to control the instruments in the system.
16 8000 Operator’s Manual
Introduction 2
8000 with Peristaltic Pump
The 8000, when used in conjunction with the peristaltic pump, is capable of performing unattended sampling from a dissolution apparatus. All parameters of operation are controlled by the 8000.
Sampling is carried out automatically and takes into account all relevant variables in the sample flow path such as tubing length, tension, and diameter. The volume calibration may be performed manually or automatically using an autocalibration block supplied with the unit.
Samples may be delivered into open test tubes or pre-capped autosampler vials.
Conventions Used in this Manual
• Items you are asked to press are in bold. For example, “press H on the keypad”.
• Key sequences you are asked to press appear like this: MENU > 7 .
8000 Operator’s Manual 17
2 Introduction
Serial Number Format
The serial number contains 10 characters and follows this syntax:
CC 12 34 xxxx
Syntax Code Meaning
CC
12
34
Country of origin
Description
2 alpha characters matching the required trade designation for the country of origin
Year of manufacture ‘09’ for 2009, ‘10’ for 2010, etc.
Week of manufacture ‘01’ for week 1 to ‘52’ for week 52
18 8000 Operator’s Manual
Agilent 8000 Dissolution Sampling Station
Operator’s Manual
3
Setting Up the 8000
Unpacking of 8000 and Components 20
Setup of 8000 and Components 34
Agilent Technologies
19
3 Setting Up the 8000
Unpacking of 8000 and Components
WARNING
Check the contents for any damages due to shipping. The packaging used to ship your equipment ensures that damage rarely occurs, but if it does, contact both the carrier who delivered the instrument and the
Dissolution Systems Service Department. Though claims for damage should be filed with the carrier, we will be glad to help you file a claim and get you up and running as quickly as possible.
For all configurations, ensure the equipment is configured for the voltage supplied.
WARNING
The electrical connection at the back of the 8000 is the primary disconnect for the instrument. The instrument should be positioned to allow accessibility to the power cords for easy disconnection.
20 8000 Operator’s Manual
Unpacking Your 8000
Setting Up the 8000 3
WARNING
Figure 1 Agilent 8000 Dissolution Sampling Station
The 8000 is shipped in foam-filled cartons. The number of cartons may vary depending on your configuration.
1 Carefully remove the 8000 and accessory kit(s) from their shipping cartons. Remove all cushioning material and tape.
2 Place the 8000 on a clear, dry, level section of the bench top close to the dissolution apparatus. The preferred placement of the 8000 is on the right side of the dissolution apparatus.
Unscrew and remove the two clamps which secured the 8000 dispensing arm during shipping. These clamps are located on both sides of the right dispensing support arm where it connects to the 8000 main body. Failure to remove the two clamps prior to powering up the 8000 may damage the unit.
8000 Operator’s Manual 21
3 Setting Up the 8000
Unpacking / Setup of 8000 & Peristaltic Pump
Environmental Requirements for Peristaltic Pump
Installation
• Altitude: max 2000m;
• Humidity: max relative humidity 80% for temperatures up to 31 ºC decreasing linearly to 50% relative humidity at 40 ºC
• Indoor use only
• Temperature: 5 ºC to 40 ºC
• Main supply voltage fluctuations are not to exceed ± 10% of the nominal supply voltage
• Operating conditions: 3 mins ON Max; 5 mins OFF Min
22
Figure 2 Peristaltic Pump
Installation
1 Carefully remove the peristaltic pump from its shipping carton.
Remove all cushioning and tape.
2 Place the peristaltic pump on a clear, dry, level section of the bench top between the 8000 and the dissolution apparatus.
3 Remove the two screws on top of the pump. Lift off the plastic cover and set it aside.
4 Remove the cartridges from the pump by pressing down the levers on the right side. Lift the cartridges up and away from the pump.
8000 Operator’s Manual
Setting Up the 8000 3
NOTE
5 Loosely fit the peristaltic pump tubing along the inside groove of the first cartridge, locking the tubing clips on the outside notches.
Ensure that all pump cartridges are installed. It is not necessary to install tubing on any unused positions.
NOTE
6 Refit the cartridge to the pump by hooking the clip on the bottom left of the cartridge into the groove on the left side of the pump.
7 Push down the right side of the cartridge, ensuring that the tubing remains under the cartridge, and pull up the lever to lock the cartridge in place. If desired, trim the excess tubing to within one inch of the tubing clips.
The cover does not fit unless the cartridges are positioned with the levers on the right side.
WARNING
8 Repeat Steps 5 - 7 for each remaining cartridge.
9 Refit the cover and replace the two screws. If the cover is not replaced correctly, the pump will not operate.
The electrical connection at the back of the 8000 is the primary disconnect for the instrument. The instrument should be positioned to allow accessibility to the power cords for easy disconnection.
10 Refer to the following example diagram to correctly make cable connections for the 8000, peristaltic pump, and dissolution
8000 Operator’s Manual 23
3 Setting Up the 8000 apparatus:
24
Figure 3 Cable Connections for 8000, Peristaltic Pump, and VK
7000 / 7010
11 Connect the five-pin cable (#2, P/N 5075-0033) between the jack on the 8000 rear panel labeled PUMP and the jack on the peristaltic pump rear panel labeled PUMP CONTROL.
12 If the system configuration includes the 705-DS, connect the 9-to-25 pin cable to the RS232 port on the dissolution apparatus and the
RS232 COMPUTER port on the 8000 rear panel.
Otherwise, connect the RS232 cable (#1, P/N 5075-0042) between the RS232 port on the dissolution apparatus and the RS232
COMPUTER port on the 8000 rear panel.
8000 Operator’s Manual
Setting Up the 8000 3
NOTE
13 The 8000 is shipped with all sample tubing attached to the valves inside the dispensing arm.
Do not remove the nylon tie wraps securing the tubing into bundles.
The direction of the peristaltic pump can be set via a switch on its rear panel. It does not matter at this point to which side the bundles go, as long as all lines in the bundle from the sampling cannulas are connected to one side, and all lines in the bundle from the valve inlets to the other.
14 If your system configuration includes the 705-DS, connect the resident probes (see Figure below) to the tubing and attach the lines to the tubing on one side of the appropriate peristaltic pump cartridges.
Otherwise, connect the tubing from the dissolution apparatus sample cannulas to the tubing on one side of the appropriate peristaltic pump cartridges.
resident probe housing sample cannula
8000 Operator’s Manual
Figure 4 Resident Probe
15 Connect another length of tubing between the remaining side of the peristaltic pump cartridges and the tubing coming from the left positions of the 8000 solenoid valves. Ensure each cartridge has identical color tubing connected at both the inlet and the outlet.
25
3 Setting Up the 8000
WARNING
16 Connect the remaining length of tubing coming from the top positions of the 8000 solenoid valves to the return lines on the dissolution apparatus. Ensure each line is attached to its identical color.
Do not tighten the plastic fittings on the ends of the tubing more than half a turn past finger-tight. Overtightening will damage the fittings.
17 For a system that includes a 705-DS, connect the return lines to the return cannulas of the resident probes.
Unpacking / Setup of 8000 & Syringe Pump
26
Figure 5 806 Syringe Pump
The syringe pump is shipped in individual packaging.
1 Carefully lift the unit out of the box.
2 Remove any cushioning material.
8000 Operator’s Manual
Setting Up the 8000 3
3 Place the 806 Syringe Pump on a clean, dry, level section of the bench top between the 8000 and the dissolution apparatus.
4 Refer to the following example diagram to correctly make cable connections for the 8000, syringe pump, and dissolution apparatus:
8000 Operator’s Manual
Figure 6 Cable Connections for 8000, Syringe Pump, and VK 7000 /
7010
5 Connect the single end of the Y cable (#1, P/N 5075-0446) to the
RS232 port on the syringe pump.
6 Connect an RS232 cable (#2, P/N 5075-0252) between the RS232
COMPUTER port on the 8000 and one extension of the Y cable attached to the syringe pump.
7 If the system configuration includes the 705-DS, connect a
9-to-9-pin cable (P/N 5075-0448) from the RS232 port on the dissolution apparatus rear panel to the remaining extension of the Y cable attached to the syringe pump.
Otherwise, connect a second RS232 cable (#2, P/N 5075-0252) between the remaining extension of the Y cable attached to the syringe pump and the RS232 port on the dissolution apparatus.
27
3 Setting Up the 8000
8 A system configuration that includes a non-Agilent dissolution apparatus, requires use of an adapter (P/N 5075-0852, see following
Figure). This adapter should be attached to the #2 port of the Y-cable in place of the cable connected to the dissolution apparatus.
28
NOTE
Figure 7 Y Cable 5075-0446 and Adapter 5075-0852
9 The 8000 is shipped with all sample tubing attached to the valves inside the dispensing arm.
Do not remove the nylon tie wraps securing the tubing into bundles.
10 If the system configuration includes the 705-DS, connect the resident probes to the sample tubing and attach the lines to the left port
(labeled IN) of each syringe starting with Position 1.
Otherwise, connect the tubing from the dissolution apparatus sampling cannulas to the left port (labeled IN) of each syringe starting with Position 1.
11 Connect a separate length of tubing between the right port (labeled
OUT) of each syringe to the tubing coming from the left positions of
8000 Operator’s Manual
Setting Up the 8000 3
WARNING the 8000 solenoid valves. Ensure each line is attached to its identical color.
12 Connect the remaining length of tubing from the top positions of the
8000 solenoid valves to the return lines on the dissolution apparatus.
Ensure each line is attached to its identical color.
Do not tighten the plastic fittings on the ends of the tubing more than half a turn past finger-tight. Overtightening will damage the fittings.
13 If the system configuration includes the 705-DS, connect the return lines to the return cannulas of the resident probes.
Unpacking / Setup of 8000, Syringe Pump, and Filter
Changer
Environmental Requirements for Filter Changer Installation
• Altitude max.2000m;
• Humidity max relative humidity 80% for temperatures up to 31 ºC decreasing linearly to 50% relative humidity at 40ºC;
• Indoor use only
• Temperature: 5 ºC to 40 ºC;
• Mains supply voltage fluctuations are not to exceed ± 10% of the nominal supply voltage.
• Operating conditions 8 mins ON Max; 5 mins OFF min
8000 Operator’s Manual 29
3 Setting Up the 8000
Installation
30
Figure 8 808 Filter Changer
The filter changer is shipped in individual packaging.
1 Carefully lift the unit out of the box.
2 Remove any cushioning material.
3 Place the unit on a clear, dry, level section of the bench top between the 8000 and the syringe pump.
8000 Operator’s Manual
Setting Up the 8000 3
4 Refer to the following example diagram to correctly make cable connections for the 8000, syringe pump, filter changer, and dissolution apparatus:
8000 Operator’s Manual
Figure 9 Cable Connections for 8000, Syringe Pump, Filter Changer, and VK 7000 / 7010
5 Connect the single end of a Y cable (#1, P/N 5075-0446) to the
RS232 port on the filter changer.
6 Connect an RS232 cable (#2, P/N 5075-0252) between the RS232
COMPUTER port on the 8000 and one extension of the Y cable attached to the filter changer.
7 Connect a 9-9 pin RS232 cable (#3, P/N 5075-0449) from the remaining extension of the Y cable attached to the filter changer to one extension of a separate Y-cable.
8 Connect the single end of the second Y cable (#1, P/N 5075-0446) to the RS232 port on the syringe pump.
9 If the system configuration includes the 705-DS, connect a
9-to-9-pin cable (P/N 5075-0448) from the RS232 port on the dissolution apparatus rear panel to the remaining extension of the Y cable attached to the syringe pump.
Otherwise, connect a second RS232 cable (#2, P/N 5075-0252)
31
3 Setting Up the 8000 between the remaining extension of the Y cable attached to the syringe pump and the RS232 port on the dissolution apparatus.
10 A system configuration that includes a non-Agilent dissolution apparatus, requires use of an adapter (P/N 5075-0852, see following
Figure). This adapter should be attached to the #2 port of the Y-cable in place of the cable connected to the dissolution apparatus.
32
NOTE
Figure 10 Y Cable 5075-0446 and Adapter 5075-0852
11 The 8000 is shipped with all sample tubing attached to the valves inside the dispensing arm.
Do not remove the nylon tie wraps securing the tubing into bundles.
12 If the system configuration includes the 705-DS, connect the resident probes to the sample tubing and attach the lines to the left port
(labeled IN) of each syringe starting with Position 1.
Otherwise, connect the tubing from the dissolution apparatus sampling cannulas to the left port (labeled IN) of each syringe
8000 Operator’s Manual
Setting Up the 8000 3 starting with Position 1.
resident probe housing sample cannula
WARNING
Figure 11 Resident Probe Housing and Sample Cannula
13 Connect a separate length of tubing from the right port (labeled
OUT) of each syringe to the top inlet ports of the filter changer starting with the port closest to the carousel.
14 Connect another separate length of tubing lines from the lower side ports of the filter changer to the tubing coming from the left positions of the 8000 solenoid valves. Ensure each line is attached to its identical color.
15 Connect the tubing coming from the top positions of the 8000 solenoid valves to the return lines of the dissolution apparatus.
Ensure each line is attached to its identical color.
Do not tighten the plastic fittings on the ends of the tubing more than half a turn past finger-tight. Overtightening will damage the fittings.
16 If the system configuration includes the 705-DS, connect the return lines to the return cannulas of the resident probes.
17 To install the catch basin for the filters, slide the side of the basin containing the opening over the screw mounted on the front of the filter changer.
8000 Operator’s Manual 33
3 Setting Up the 8000
Setup of 8000 and Components
Installing 8000 Full Flow Filters
The 8000 has been supplied with 10-, 35-, and 70-micron Full Flow
Filters for use with the sampling cannulas. In theory, dissolution continues until the samples are filtered. The 8000 allows filtering at the instant a sample is taken with the use of the Full Flow Filters. Some drug excipients may clog the Full Flow filters reducing their performance. Filters may need to be changed during a run only in extreme cases.
WARNING
Install Full Flow Filters on the sampling cannulas to prevent clogging of the sampling lines. Damage to your equipment will result if the sampling lines clog. For best results, use the
10-micron Full Flow Filter.
Full Flow Filters are not for use with Apparatus 3 / 7 configurations.
Power and Network Connections
WARNING
The electrical connection at the back of the 8000 is the primary disconnect for the instrument. The instrument should be positioned to allow accessibility to the power cords for easy disconnection.
Ensure the 8000 and any coupled components are configured for the voltage supplied at the receptacle.
• If your system configuration includes the peristaltic pump, the power receptacle, and power switch are located on the rear of the unit.
Connect the power cord and turn on the peristaltic pump.
34 8000 Operator’s Manual
Firmware version
Setting Up the 8000 3
• If your system configuration includes the syringe pump, the power receptacle, and power switch are located on the left side of the unit.
Connect the power cord and turn on the syringe pump.
• If your system configuration includes the filter changer, the power receptacle, and power switch are located on the rear of the unit.
Connect the power cord and turn on the filter changer.
The 8000 has a dual voltage (115 V / 230 V) option on the power entry module. Ensure the voltage indicated on the fuse drawer matches the power outlet. Connect the power cord to the rear of the 8000. Turn on the 8000. The system monitor illuminates. The system monitor displays the initial status screen and then changes to the Ready screen.
8000 FRACTION COLLECTOR
PROGRAM REVISION x.xx
INITIALIZING....PLEASE WAIT
TIME: 01/01/12 21:56:40 ELAPSE: 000:00:00
The firmware version (program revision) displays. This is the only time the firmware version displays. Record the number below and refer to it if you need to call the Dissolution Systems Service Department.
While the unit is powering up, the dispensing arm returns to its home position at the rear of the unit and the firmware program initializes. If the dispensing arm is already at the home position when the unit is powered up, the initial status screen displays for three seconds only.
When the initial status screen disappears, the Ready screen displays.
From the Ready screen, you can access the Main Menu or any of the manual functions.
READY
PRG# 01 R-M: ENABLED VOLUME: 5 mL
TIME: 01/01/12 21:56:40 ELAPSE: 000:00:00
8000 Operator’s Manual 35
3 Setting Up the 8000
Installing the Sample Tray
Place the supplied sample tray on top of the unit. The sample tray has four grommeted corner holes which fit over the four locating pins.
WARNING
The sample tray must be securely mounted on the locating pins.
Otherwise, damage can occur to the sampling needles and drive mechanism.
Installing the Media Rinse Reservoir
The media rinse reservoir fits in the rear of the unit between the metal brackets. The dispensing arm must be moved forward to put it in place.
1 From the Ready screen, press MENU .
2 Select option 7 , Manual Operation.
3 Select option 4 , Goto Rows.
4 Enter 4 to move the dispensing arm to Row 4. The dispensing arm moves forward to allow access to the rear of the 8000.
5 Place the media rinse reservoir between the metal brackets directly behind the sample tray.
NOTE
The media rinse reservoir has two chambers—one for replacement media and one for rinsing. The replacement media chamber has two barbed fittings—one on each end of the tank. The rinse chamber has a single barbed fitting. Orient the reservoir with the rinse chamber toward the front.
6 Connect the six-foot length of clear Tygon tubing to the barbed elbow fitting located on the rinse chamber of the reservoir. Place the other end in a sink, drain, or suitable waste container.
7 If your system configuration includes the syringe pump, press H to move the dispensing arm to the home position and press ESC three times to return to the Ready screen.
36 8000 Operator’s Manual
Setting Up the 8000 3
If your system configuration includes the peristaltic pump, complete the
steps under “Installing and Connecting the Autocalibration Block
(Peristaltic Pump only)” on page 37.
Installing and Connecting the Autocalibration Block
(Peristaltic Pump only)
1 With the dispensing arm forward, insert the autocalibration block behind the media rinse reservoir. Ensure the RS232 port is positioned to the right for a 6-row configuration and toward the back for a
12-row configuration.
2 Connect the nine-pin RS232 cable between the CALIBRATION port on the 8000 rear panel and the RS232 port on the side of the autocalibration block.
3 Press H to move the dispensing arm to the home position.
4 Press ESC three times to return to the Ready screen.
8000 Operator’s Manual 37
3 Setting Up the 8000
Installing the RM Module
Internal Uni
t
Pump 1
Pump 1
Pump 2
Pump 2
Figure 12 Internal Media Replacement Module
1 Attach a length of 1/2-inch diameter tubing to the IN fitting of pump
1 (the top pump).
2 Place the free end in one of the unstirred vessels containing medium or in the receptacle being used to hold the replacement medium.
3 If desired, attach a clip to regulate flow speed on this length of tubing.
4 Connect a length of tubing from the OUT fitting of pump 1 to the IN fitting on the RM chamber of the media rinse reservoir.
5 Attach a length of tubing between the OUT fitting of the RM chamber and the IN fitting on pump 2 (the bottom pump).
6 Attach a length of tubing to the OUT fitting on pump 2 and place the free end in the replacement medium vessel or receptacle.
38 8000 Operator’s Manual
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Setting Up the 8000 3
External Unit
Figure 13 External Media Replacement Module
1 Remove the cover housing the RM chamber from the side of the
8000.
2 Detach the RM chamber.
3 Insert the end of the tubing attached to the IN fitting on the pump into the plastic RM chamber.
4 Slide a tubing clamp on a length of tubing.
5 Connect one end of the tubing containing the clamp to the OUT fitting on the pump. Connect the other end to the IN fitting of the RM chamber.
6 Connect a length of plastic tubing to the OUT fitting of the RM chamber.
7 Place the free end of this piece in the plastic RM chamber.
8 Reattach the RM chamber, tighten all screws, and replace all covers.
39
3 Setting Up the 8000
Loading Filters (Filter Changer only)
1 Choose a tube of filters. Although you can load up to eight tubes of filters, only one tube is necessary for the filter changer to function properly.
2 Remove the cap from the end of the tube to expose the long, thin exit nozzle of the end filter.
3 Insert the tube, with the open end down, into the metal filter assembly in the filter changer carousel. Pivot the tube until it slides vertically into the white stabilizer slot.
4 Repeat Steps 2 - 4 to load additional tubes of filters.
WARNING
Use only Agilent-certified filters or Millipore-equivalent types of filters. Other types of filters may not work properly in the filter changer and could damage the unit.
40
Changing Filter Types (Filter Changer Only)
1 Remove all tubes of filters from the carousel of the filter changer.
2 From the 8000 Ready screen, press MENU . The Main Menu displays.
3 Select option 7 , Manual Operation. The Manual Operations screen displays.
4 Select option 6 , Syringe/Filter.
5 From the Operate Syringe/Filter Changer screen, select option 5 ,
Filter Changer. The Filter Changer Manual Control screen displays.
6 Select option 3 , Change Filters.
7 Enter 8 as the number of filters to change and press ENTER . The filters expel from the filter changer.
8 Repeat Steps 6 and 7 until filters no longer expel from the filter changer. The following error message displays on the screen: FILTER
CHANGER ROTOR ERROR . THE FILTER CHANGER IS EMPTY OR HAS
FAILED TO LOAD PROPERLY . PRESS <1> TO CONTINUE OR < ESC > TO
QUIT .
9 Press ESC . The Filter Changer Manual Control screen displays.
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Setting Up the 8000 3
10 Load the new tubes of filters into the carousel of the filter changer.
See “Loading Filters (Filter Changer only)” on page 40.
11 Select option 3 , Change Filters.
12 Enter 8 as the number of filters to change and press ENTER . The filters expel from the filter changer.
13 When the filter changer stops expelling filters, select option 3 ,
Change Filters, again.
14 Enter 1 as the number of filters to change and press ENTER . The remaining filter of the previous type expels from the filter changer and is replaced with the new filter.
15 Press ESC four times to return to the Ready screen.
Hidden Key Functions
Table 1 Hidden Key Functions
Key Sequence
MENU > 0 > 0
MENU > 0 > 8
MENU > 0 > A
MENU > 0 > B
MENU > 0 > C
Function
Use this key sequence to select the system configuration. Select the option that corresponds to the number of valves on your 8000.
Use this key sequence to select a fixed 1200 baud or a programmable baud. Select option 2 , Programmable Baud.
Note: If you are using the 8000 with other Agilent equipment, the baud rate must be
set to 9600 baud (see “ Printer Operation and Communications ” on page 103
).
Use this key sequence to set the drop volume before sampling. Enter the desired drop volume and press ENTER . The maximum acceptable value is 1.
Note: The drop volume is an amount of sample that is dispensed through the 8000 needles prior to dispensing samples into the collection tubes to ensure the needles are purged completely.
Use this key sequence to enable or disable the moving alert. The asterisk (*) displays next to the current selection. If enabled, the alarm sounds when the dispensing arm is in motion.
Use this key sequence to enable or disable the delayed start alarm. The asterisk (*) displays next to the current selection. If the delayed start alarm is enabled, the alarm
sounds prior to the start of the program. See “DISSO Options Screen” on page 69.
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3 Setting Up the 8000
Table 1 Hidden Key Functions
Key Sequence
MENU > 0 > D
MENU > 0 > E
MENU > 0 > F
MENU > 0 > P
MENU > 0 > R
Function
Use this key sequence to enable or disable the multiple sample option. The asterisk (*) displays next to the current selection.
Note: For use with APP 3/7 (Apparatus 3 / 7) systems only.
Use this key sequence to select the printer type. Ensure the asterisk (*) displays next to option 1, Impact. If not, select option 1 to enable the impact printer.
Note: Select option 1, Impact, or the instrument will not operate properly.
Use this key sequence to select the date display style. The asterisk (*) displays next to the current selection. Select option 1 , MM/DD/YY, or option 2 , DD/MM/YY.
Use this key sequence to enable or disable the syringe pump. The asterisk (*) displays next to the current selection.
If your system configuration includes the syringe pump, you must enable the syringe pump control options on the 8000 prior to setting the syringe pump parameters (see
Use this key sequence to enable or disable media replacement for the syringe pump.
The asterisk (*) displays next to the current selection. Select option 1 , Enable, or option 2 , Disable, as appropriate.
Note: This option is not available for APP 3/7 (Apparatus 3 / 7) systems.
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8000 Operator’s Manual 43
3 Setting Up the 8000
44 8000 Operator’s Manual
Agilent 8000 Dissolution Sampling Station
Operator’s Manual
4
Operating the 8000
Operating the 8000 with Apparatus 1 / 2 46
Operating the 8000 with Apparatus 3 / 7 72
Volumetric Calibration (Peristaltic Pump only) 97
Printer Operation and Communications 103
Agilent Technologies
45
4 Operating the 8000
Operating the 8000 with Apparatus 1 / 2
8000 Keypad Options
The keypad on the 8000 is similar to a personal computer keyboard. For example, it has a SPACE key, an ENTER key, and a SHIFT key. These keys function exactly like their counterparts on a personal computer or
typewriter keyboard. See Figure 14, “8000 Keypad,” below. A
discussion about each of the main function keys follows.
Figure 14 8000 Keypad
46 8000 Operator’s Manual
Operating the 8000 4
The front panel options include the following:
Table 2 Front Panel Options (Apparatus 1 / 2)
Option
ESC
MENU
CAL
PROG
START PROG
MANUAL SAMPLE
OPEN VALVES
Function
Press ESC to stop execution of a running program or calibration routine. Press
ESC to move back one level to the previous menu or selection when moving through the multi-level menu.
Note: Be very careful when using ESC while a program is running. If a program or calibration is running and ESC is pressed, a prompt appears on the first line of the display asking if you want to stop the running program. The options are Y or
N. Press Y to stop the running program. Press N to continue the running program.
Use this option to set the clock, alarms, DISSO parameters, start output delay / syringe pump parameters, manual prime time / volume, manual purge time / volume as well as to remotely control the dissolution apparatus and manually control the 8000.
Press PRINT to record the batch information, print the sample times, enable or disable the Report Center Printer, select a local or remote printer, set the communication port identification number, and set the baud rate.
Use this option to calibrate the 8000 to ensure that sample volumes are accurately and precisely taken.
Note: The CAL key on the 8000 can be used only if the peristaltic pump is installed on your system.
Use this option to set program parameters.
Use this option to start a program.
Use this option to collect a sample from the dissolution vessels on command without a program.
Press OPEN VALVES to open all valves simultaneously. This function keeps the valves open as long as the key on the keypad is pressed. To change the valve configuration, press MENU > 0 > 0 and select the appropriate configuration.
(PROBES UP / DOWN)
Press ( PROBES UP / DOWN ) to raise or lower the sampling cannulas on the dissolution apparatus.
Note: Not available with APP 3/7 systems.
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4 Operating the 8000
Table 2 Front Panel Options (Apparatus 1 / 2)
Option
PUMP OFF
PUMP FWD
PUMP REV
CLEAN SYTEM
LOCK KBRD
Function
PUMP OFF, PUMP FWD, and PUMP REV work exclusively with the Agilent peristaltic pump. When the syringe pump is connected, they are disabled.
Use this option to keep the valves, needles, and fluid lines clean.
Press LOCK KBRD to lock the keypad. Press LOCK KBRD again to unlock the keypad.
8000 Menu Options - Apparatus 1 / 2
Main Menu
From the Ready screen, press MENU . The Main Menu displays.
Options 3, 4, and 5 change depending on your system configuration.
When your system configuration includes the peristaltic pump, the following Main Menu displays:
***MAIN MENU***
1 SET CLOCK/ALARMS
2 SET DISSO
3 START OUTPUT DELAY
4 MANUAL PRIME TIME
5 MANUAL PURGE TIME
6 CONTROL DISSO
7 MANUAL OPERATION
When your system configuration includes the syringe pump with or without the filter changer, the following Main Menu displays:
***MAIN MENU***
1 SET CLOCK/ALARMS
2 SET DISSO
3 SET SYRINGE PUMP
4 MANUAL PRIME VOL.
5 MANUAL PURGE VOL.
6 CONTROL DISSO
7 MANUAL OPERATION
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Operating the 8000 4
Following is a description of the Main Menu screen options:
Table 3 Main Menu Options (Apparatus 1 / 2)
Option
1 Set Clock/Alarms
2 Set DISSO
3 Start Output Delay
(peristaltic pump)
3 Set Syringe Pump
(syringe pump)
4 Manual Prime Time
(peristaltic pump)
Function
Use this option to set the clock and alarms. If your system configuration includes the syringe pump, the start output delay option also displays on this screen. See
“Main Menu Option 1 - Set Clock/Alarms” on page 51.
Use this option to set the DISSO parameters. See “Main Menu Option 2 - Set
DISSO” on page 53.
If your system configuration includes the peristaltic pump, use this option to set the delay from the start of a program until the TTL signal is sent from the START
OUTPUT jack on the 8000 rear panel.
Select option 3 , Start Output Delay. Enter a delay length of up to 99:59 minutes for the 8000 TTL output and press ENTER . The Main Menu displays.
Note: This output signal can be used to start other instrumentation, such as a dissolution apparatus, after the program on the 8000 is started. The default delay is 00:00, indicating that the signal will be sent at the moment a program starts.
For example, a value of 10:00 delays the signal for ten minutes after the program starts. Set the value to 00:00 to eliminate the delay feature.
When the syringe pump is enabled, Start Output Delay displays under the Set
Clock and Alarms screen as option 6. See “6 Start Output Delay” on page 52.
If your system configuration includes the syringe pump, use this option to set the syringe pump parameters. See “Main Menu Option 3 - Set Syringe Pump” on page 55.
If your system configuration includes the peristaltic pump, use this option to set the interval the peristaltic pump runs forward, drawing medium into the system, before the valves open to deliver samples.
Select option 4 , Manual Prime Time. Enter a value for the priming time from 1 to
99 seconds and press ENTER . The Main Menu displays.
Note: At a minimum, the manual prime time should be long enough for medium to be seen dripping from the return cannulas. In general, the longer the tubing lengths, the longer the priming time required. This value is not used by sampling programs, which have their own individual priming time values. It is used only with the MANUAL SAMPLE, CAL, and CLEAN SYSTEM keys on the 8000.
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4 Operating the 8000
Table 3 Main Menu Options (Apparatus 1 / 2)
Option
4 Manual Prime Vol.
(syringe pump)
5 Manual Purge Time
(peristaltic pump)
5 Manual Purge Vol.
(syringe pump)
6 Control DISSO
7 Manual Operation
Function
If your system configuration includes the syringe pump, use this option to set the amount of drawn medium necessary to fill the sampling lines of the entire system.
Select option 4 , Manual Prime Vol. Enter a value for the priming volume and press ENTER . The Main Menu displays.
Note: At a minimum, the manual prime volume should be large enough for medium to be seen dripping from the return cannulas. In general, the longer the tubing lengths, the larger the priming volume required. This value is not used by sampling programs which have their own individual priming volume values. It is used only with the MANUAL SAMPLE and CLEAN SYSTEM keys on the
8000.
If your system configuration includes the peristaltic pump, use this option to set the interval the peristaltic pump runs in reverse to return the uncollected medium in each line back into the vessel from which it was drawn.
Select option 5 , Manual Purge Time. Enter a value for the manual purge time from 1 to 99 seconds and press ENTER . The Main Menu displays.
Note: Purging also clears the sampling filters of particulate matter that may restrict successive samples. As with priming, the longer the tubing lengths, the longer the purge time required. This value is not used by sampling programs, which have their own individual purging time values. It is used only with the
MANUAL SAMPLE, CAL, and CLEAN SYSTEM keys on the 8000.
If your system configuration includes the syringe pump, use this option to set a purge volume that ensures all stranded medium is properly expelled.
Select option 5 , Manual Purge Vol. Enter a value for the purge volume that ensures at least two plunger strokes and press ENTER . The Main Menu displays.
Note: The first stroke moves all medium from the sampling lines to the return lines. The second stroke draws air into the sampling lines and purges the remaining medium from the return lines. As with priming, the longer the tubing lengths, the larger the purge volume required. This value is not used by sampling programs which have their own individual purging volume values. It is used only with the MANUAL SAMPLE and CLEAN SYSTEM keys on the 8000.
Use this option to remotely control the dissolution apparatus from the 8000. See
“Main Menu Option 6 - Control DISSO” on page 57.
Use this option to manually control the 8000. See “Main Menu Option 7 -
Manual Operation” on page 57.
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Operating the 8000 4
Main Menu Option 1 - Set Clock/Alarms
From the Main Menu, select option 1 , Set Clock/Alarms. The Set Clock and Alarms screen displays.
1 SET CLOCK
***SET CLOCK AND ALARMS***
2 TIMER ALARM
3 ROW COMPL ALARM
5 PRG COMPL ALARM
4 CALIB. COMPL ALARM
6 START OUTPUT DELAY
Following is a description of the Set Clock and Alarms screen options:
Table 4 Set Clock and Alarms Options (Apparatus 1 / 2)
Option
1 Set Clock
2 Timer Alarm
3 Row Compl Alarm
Function
Select option 1 , Set Clock. Enter the date in the appropriate format and press
ENTER . The time displays in 24-hour format. If the time is correct, press
ENTER or ESC to return to the Main Menu. If the time is incorrect, enter the correct time in 24-hour format and press ENTER . The Set Clock and Alarms screen displays.
Use this option to set the elapsed timer alarm. The elapsed timer alarm is a simple timer you can use to time any interval from 1 minute to 99 hours. The alarm sounds when the time has expired. Once turned off, the timer does not operate again until you reset it.
Select option 2 , Timer Alarm. Enter a duration in hh:mm format and press
ENTER . The Set Clock and Alarms screen displays.
Press any key to silence the alarm after it sounds.
Use this option to set an alarm to indicate the completion of each sample point.
This alarm is useful when the samples must be checked or removed for immediate analysis.
Select option 3 , Row Compl Alarm. Select option 1 to enable or option 2 to disable the alarm. The Set Clock and Alarms screen displays.
Press any key to silence the alarm after it sounds.
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4 Operating the 8000
Table 4 Set Clock and Alarms Options (Apparatus 1 / 2)
Option
4 Calib. Compl Alarm
5 Prg Compl Alarm
6 Start Output Delay
Function
Use this option to set an alarm to sound when autocalibration is complete and all lines are purged.
Select option 4 , Calib. Compl Alarm. Select option 1 to enable or option 2 to disable the alarm. The Set Clock and Alarms screen displays.
Press any key to silence the alarm after it sounds.
Note: This option is only available if your system configuration includes the peristaltic pump.
Use this option to set an alarm to sound at the end of the program. It is a useful reminder that the program is complete.
Select option 5 , Prg Compl Alarm. Select option 1 to enable or option 2 to disable the alarm. The Set Clock and Alarms screen displays.
Press any key to silence the alarm after it sounds.
Note: Option 6 displays only when the syringe pump is enabled.
If the syringe pump is enabled, use this option to set the delay from the start of a program until the TTL signal is sent from the START OUTPUT jack on the 8000 rear panel.
Select option 6 , Start Output Delay. The Start Output Delay screen displays. Enter a delay length of up to 99:59 minutes for the 8000 TTL output and press ENTER .
The Set Clock and Alarms screen displays.
Note: This output signal can be used to start other instrumentation, such as a dissolution apparatus, after the program on the 8000 is started. The default delay is
00:00, indicating that the signal will be sent at the moment a program starts. For example, a value of 10:00 delays the signal for ten minutes after the program starts. Set the value to 00:00 to eliminate the delay feature.
When the syringe pump is disabled, this option displays under the Main Menu.
See “3 Start Output Delay (peristaltic pump)” on page 49 .
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Main Menu Option 2 - Set DISSO
From the Main Menu, select option 2 , Set DISSO. Enter a program number and press ENTER . The DISSO Parameters screen displays.
***DISSO PARAMETERS***
1 SET SPINDLE RPM
2 FINAL SPIN LENGTH
3 INI/FINAL VESSEL TEMP
4 SET BATH TEMP (VESSEL TEMP)
5 COM PORT ID
6 DDM OR BASKET
7 PRINT FREQ
NOTE
DISSO applies to all dissolution apparatus.
Following is a description of the DISSO Parameters screen options:
Table 5 Disso Parameters Options (Apparatus 1 / 2)
Option
1 Set Spindle RPM
2 Final Spin Length
3 Ini/Final Vessel Temp
Function
Select option 1 , Set Spindle RPM. The Parameter for Manual or Program screen displays.Select option 1 , Manual, to set an RPM to be used as the default for manual operation (see “Main Menu Option 6 - Control DISSO” on page 57). Optionally, select option 2 , Program, to set an RPM to be used as a default for programmed
operation (see “Starting a 8000 Program” on page 66). Enter the desired RPM and
press ENTER . The DISSO Parameters screen displays.
Select option 2 , Final Spin Length, to program a short, high-speed spin cycle at
250 RPM at the end of the test. Enter a duration for the final spin and press ENTER .
The DISSO Parameters screen displays.
Note: Many dissolution protocols now call for an infinity sample to be taken after the end of the test.
Select option 3 , Ini/Final Vessel Temp. The Initial Vessel Temp Measurement screen displays. Select option 1 to enable or option 2 to disable the initial vessel temperature reading. The Final Vessel Temp Measurement screen displays. Select option 1 to enable or option 2 to disable the final vessel temperature reading. The DISSO
Parameters screen displays.
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4 Operating the 8000
Table 5 Disso Parameters Options (Apparatus 1 / 2)
Option
4 Set Bath Temp
4 Vessel Temp
5 Com Port ID
6 DDM or Basket
7 Print Freq
Function
If your system configuration includes a dissolution apparatus with a water bath, select option 4 , Set Bath Temp. If your system configuration includes a dissolution apparatus with Direct Vessel Heating (DVH), select option 4 , Vessel Temp.
Select option 1 , Manual, to set the water bath / vessel temperature. Optionally, select option 2 , Program, to set a desired water bath / vessel temperature to download to the dissolution apparatus. Enter the desired temperature and press ENTER . The DISSO
Parameters screen displays.
Select option 5 , Com Port ID, to change the communication port identification number. Your system was shipped with the communication port identification number set to 01. You can set the identification number to any number between 1 and 99. The identification number is used when multiple units are connected in series so that the controlling or master 8000 can start individual units.
Enter the communication port identification number and press ENTER . The DISSO
Parameters screen displays.
Select option 6 , DDM or Basket, to select the apparatus type. The Select Basket or
Paddle of DISSO screen displays.
If using baskets, select option 1 , Basket. The DISSO Parameters screen displays.
If using paddles and sequential delivery, select option 2 , Paddle. The Dosage Delivery
Mode of DISSO screen displays. Select option 1 , Sequential, enter a time interval for the dosage drop and press ENTER . The DISSO Parameters screen displays.
Note: Only use the sequential delivery option if your dissolution apparatus is equipped with clutches.
If using paddles and simultaneous delivery, select option 2 , Paddle. The Dosage
Delivery Mode of DISSO screen displays. Select option 2 , Simultaneous. The DISSO
Parameters screen displays.
The dissolution apparatus status prints at each sample time point. Select option 7 ,
Print Freq, to set additional times to print the dissolution apparatus status. This option should be reserved for use when there is more than 30 minutes between sample time points.
Enter a print frequency and press ENTER . The DISSO Parameters screen displays.
The print frequency timer restarts after each sample time point.
Note: The status does not print if the specified print frequency is less than three minutes from the sample time point; the sample time point takes precedence.
54 8000 Operator’s Manual
Operating the 8000 4
NOTE
Once all values have been entered, press ESC . A confirmation screen displays. Press Y to send the newly entered configuration to the dissolution apparatus and override the existing settings.
Main Menu Option 3 - Set Syringe Pump
This section is applicable only to systems including a syringe pump.
Ensure the syringe pump is enabled prior to setting the syringe pump parameters (see MENU > 0 > P under “Hidden Key Functions” on page
53).
From the Main Menu, select option 3 , Set Syringe Pump. Select option
1 to enable the syringe pump. Once enabled, the Set Syringe Pump
Parameters screen displays.
***SET SYRINGE PUMP PARAMETERS***
1 SYRINGE SIZE 2 PLUNGER SPEED
3 PRIME LOSS VOLUME 4 ASPIRT. DWELL TIME
Following is a description of the Set Syringe Pump Parameters screen options:
Table 6 Set Syringe Pump Parameters (Apparatus 1 / 2)
Option
1 Syringe Size
2 Plunger Speed
Function
Use this option to set the syringe size.
Select option 1 , Syringe Size. Select the syringe size that corresponds with the
Kloehn syringes loaded into the syringe pump. The Set Syringe Pump Parameters screen displays.
Use this option to configure the syringe pump plunger to operate at a specific speed.
Select option 2 , Plunger Speed. Enter a plunger speed between 100 and 1500 motor steps per second (sps) and press ENTER . The recommended value is 500.
The Set Syringe Pump Parameters screen displays.
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4 Operating the 8000
Table 6 Set Syringe Pump Parameters (Apparatus 1 / 2)
Option
3 Prime Loss Volume
4 Aspirt. Dwell Time
5 Full Stroke (hidden option)
6 Pump Current (hidden option)
Function
Use this option to configure the prime loss volume. Prime loss volume compensates for the volume of medium used to fill the tubing between the sampling cannulas and sampling valves during the priming cycle. When sampling, an amount of sample equal to the prime loss volume flows past the valves in the
8000 before the valves open to collect sample. The program also restricts this volume to be less than or equal to the syringe size volume.
Select option 3 , Prime Loss Volume. Enter a prime loss volume based on the sample tubing length, inner diameter, and system dead volumes and press
ENTER . The Set Syringe Pump Parameters screen displays.
If the tubing length between the sampling cannulas and sampling valves is changed, adjust the prime loss volume accordingly.
Use this option to configure the aspiration dwell time. Aspiration dwell time is the time the syringe plunger pauses at the bottom of a stroke to allow the medium being drawn to overcome the vacuum generated by the drawn plunger.
Select option 4 , Aspirt. Dwell Time. Enter an aspiration dwell time between 1 and
10 seconds and press ENTER The recommended time is 10 seconds. The Set
Syringe Pump Parameters screen displays.
Use this option to configure the number of steps the stepper motor makes to draw the syringe plunger its maximum stroke length.
Select option 5 , Full Stroke. Enter a full stroke value between 0 and 19999 and press ENTER . The recommended value is 19300. The Set Syringe Pump
Parameters screen displays.
If the volume dispensed by the syringe is outside of the required specifications, the full stroke value can be adjusted using the following calculation and solving for x: target volume (mL) = avg volume dispensed (mL)
x 19300
Use this option to configure the pump current. The use of more viscous media requires a higher pump current.
Select option 6 , Pump Current. Enter a pump current between 0 and 8 and press
ENTER . The recommended value is 4. The Set Syringe Pump Parameters screen displays.
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Main Menu Option 6 - Control DISSO
From the Main Menu, select option 6 , Control DISSO. The DISSO
Control/Monitor screen displays.
***DISSO CONTROL/MONITOR***
1 RUN 2 STOP 3 LIFT 4 LOWER 5 STILL
SET RPM: 100.0
SET BATH TEMP: 37.5
ACTUAL RPM: 000.0
ACTUAL TEMP: 37.5
The following options are commands communicated from the 8000 to the dissolution apparatus:
Table 7 Disso Control/Monitor Options (Apparatus 1 / 2)
Option
1 Run
2 Stop
3 Lift
4 Lower
5 Still
Function
Select option 1 , Run, to start the paddle or basket rotation at the last set RPM.
Select option 2 , Stop, to stop the rotation of the paddles or baskets.
Select option 3 , Lift, to raise the dissolution apparatus drive unit.
Select option 4 , Lower, to lower the dissolution apparatus drive unit.
Select option 5 , Still, to stop the upward or downward movement of the dissolution apparatus drive unit.
CAUTION
All functions are executed immediately without pressing ENTER. Press
ESC to return to the Main Menu.
After selecting option 3, Lift, or option 4, Lower, the drive unit can only be stopped by selecting option 5, Still, or by pressing DRIVE
UP, DRIVE DOWN, or LIFT p or q on the dissolution apparatus.
Main Menu Option 7 - Manual Operation
From the Main Menu, select option 7 , Manual Operation.
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4 Operating the 8000
The following Manual Operations screen displays when your system configuration includes the peristaltic pump:
***MANUAL OPERATIONS***
1 LIFT VALVES 2 LOWER VALVES
3 OPEN/CLOSE VALVES
5 TURN ON (OFF) R-M PUMP
4 GOTO ROWS
The following Manual Operations screen displays when your system configuration includes the syringe pump with or without the filter changer:
***MANUAL OPERATIONS***
1 LIFT VALVES
3 OPEN/CLOSE VALVES
5 TURN ON (OFF) R-M PUMP
2 LOWER VALVES
4 GOTO ROWS
6 SYRINGE/FILTER
All functions execute immediately without pressing ENTER. Press ESC to return to the Main Menu.
Following is a description of the Manual Operations screen options:
Table 8 Manual Operations Options (Apparatus 1 / 2)
Option
1 Lift Valves
2 Lower Valves
3 Open/Close Valves
4 Goto Rows
Function
Select option 1 , Lift Valves, to raise the valve needles if lowered.
Select option 2 , Lower Valves, to lower the valve needles if raised.
Select option 3 , Open/Close Valves, to toggle the valves open and closed.
Select option 4 , Goto Rows, to move the 8000 dispensing arm to a specific row. Enter a row number. The dispensing arm moves to the indicated row and stops.
If the moving alert is enabled, the alarm sounds when the dispensing arm moves. To disable the alert, see MENU > 0 > B under
58 8000 Operator’s Manual
Operating the 8000 4
Table 8 Manual Operations Options (Apparatus 1 / 2)
Option
5 Turn On (Off) R-M
Pump
6 Syringe/Filter (syringe pump only)
Function
Select option 5 , Turn On (Off) R-M Pump, to toggle the replacement media option off and on, if installed.
Use this option to manually control the syringe pump and filter changer as applicable. See “Manual Operation Option 6 - Syringe/Filter” below.
Manual Operation Option 6 - Syringe/Filter
From the Manual Operations screen, select option 6 , Syringe/Filter. The
Operate Syringe/Filter Changer screen displays.
***OPERATE SYRINGE/FILTER CHANGER***
1 VALVE TO INPUT 2 VALVE TO OUTPUT
3 SYRINGE FILL
5 FILTER CHANGER
4 SYRINGE DISPENSE
6 SYRINGE PARAMETERS
Following is a description of the Operate Syringe/Filter Changer screen options:
Table 9 Operate Syringe/Filter Changer Options (Apparatus 1 / 2)
Option
1 Valve to Input
2 Valve to Output
Function
The syringe pump contains a valve to control the direction of the flow of media.
Use this option to move the valve to the input position to manually input or fill the syringe with media.
Select option 1 , Valve to Input, to move the valve to the input position. The valve emits a loud click when it moves. If you hear no sound, the valve is already in the input position.
The syringe pump contains a valve to control the direction of the flow of media.
Use this option to move the valve to the output position to manually output or purge the syringe of media.
Select option 2 , Valve to Output, to move the valve to the output position. The valve emits a loud click when it moves. If you hear no sound, the valve is already in the output position.
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4 Operating the 8000
Table 9 Operate Syringe/Filter Changer Options (Apparatus 1 / 2)
Option
3 Syringe Fill
4 Syringe Dispense
5 Filter Changer
6 Syringe Parameters
Function
Use this option to draw a specific amount of medium into the syringes. Ensure the valve is in the input position before drawing the medium ( “1 Valve to Input” on page 59).
Select option 3 , Syringe Fill. Enter the desired percentage of each syringe to fill and press ENTER .
The syringes draw the specified amount of medium.
Use this option to dispense medium from the syringes.
Select option 4 , Syringe Dispense. The syringes dispense the previously drawn medium (see “3 Syringe Fill” above).
Use this option to manually operate the filter changer.
Select option 5 , Filter Changer. The Filter Change Manual Control screen displays.
To open the filter clamp, select option 1 , Open Clamp.
To close the filter clamp, select option 2 , Close Clamp.
To change the filters, select option 3 , Change Filters. Enter the number of filters to change and press ENTER . The specified number of filters are expelled and replaced.
Press ESC to return to the Operate Syringe/Filter Changer screen.
Use this option to configure the syringe pump. Select option 6 , Syringe
Parameters. The Set Syringe Pump Parameters screen displays. For a description of the screen options, see “Main Menu Option 3 - Set Syringe Pump” on page 55.
Note: The syringe pump parameters are global. These parameters can be entered under option 3 of the Main Menu or option 6 of the Operate Syringe/Filter
Changer screen.
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Creating a Sample Program
NOTE
Programs are not write-protected. Check with other users before proceeding.
To program the dissolution apparatus with the 8000, you must enable external control on the dissolution apparatus and log out. For more information, see the applicable Operator’s Manual for your dissolution apparatus.
NOTE
1 From the Ready screen, press PROG to program the 8000.
2 Enter a program number and press ENTER . The Program Variables screen displays, as shown on page 61 . Each option is explained on the following pages.
The 8000 can store up to 15 programs in non-volatile memory. Valid program numbers are 1 to 15. If 0 (zero) or a number larger than 15 is entered, the system continues to prompt you until a valid number is entered.
The following Program Variables screen displays when your system configuration includes the peristaltic pump:
***PROGRAM VARIABLES***
1 SET DISSO PARAMETERS
2 SAMPLE TIME POINTS
3 SAMPLE VOLUME
4 HEADERS
5 PRIME TIME
6 PURGE TIME
7 R-M
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The following Program Variables screen displays when your system configuration includes the syringe pump with or without the filter changer:
***PROGRAM VARIABLES***
1 SET DISSO PARAMETERS
2 SAMPLE TIME POINTS
3 SAMPLE VOLUME
4 HEADERS
5 PRIME VOLUME
6 PURGE VOLUME
7 FILTER CHG or R-M
NOTE
If your system configuration includes the syringe pump and filter changer, option 7 displays as Filter Chg. See “7 Filter Chg (syringe pump and filter changer)” on page 64 .
If your system configuration includes the syringe pump but not the filter changer, option 7 displays as R-M. See “7 R-M (syringe pump)” on page 64 .
Following is a description of the Program Variables screen options:
Table 10 Program Variables Options (Apparatus 1 / 2)
Option
1 Set DISSO Parameters
2 Sample Time Points
3 Sample Volume
Function
Use this option to set the dissolution apparatus parameters. Select option 1 , Set
DISSO Parameters. The DISSO Parameters screen displays. See “Main Menu Option
2 - Set DISSO” on page 53 for a description of the DISSO Parameters screen options.
Use this option to enter the sample time points. See “Program Option 2 - Sample
Time Points” on page 65 .
Select option 3 , Sample Volume. Enter the sample volume in milliliters and press
ENTER . The program Variables screen displays. You can enter the volume with up to three digits (for example 13.5) so that fractional milliliters can be collected. Verify the collection tubes can hold the requested volume.
Note: The maximum volume accepted is 14 mL.
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Table 10 Program Variables Options (Apparatus 1 / 2)
Option
4 Headers
5 Prime Time (peristaltic pump)
5 Prime Volume (syringe pump)
6 Purge Time (peristaltic pump)
Function
Select option 4 , Headers. The Batch # Selections screen displays. For each option, enter the desired information and press ENTER . Press ESC to return to the Program
Variables screen.
Note: The Batch # Selections screen displays options for the report header information. The print field accepts ten characters. Characters can be letters, numbers, special characters or spaces. The information entered for each selection prints in the header of each printout. The entries made from this menu are attached to the active program. Since there are 15 programs, there can be a different set of entries for each. All are stored in the battery-protected memory and remain until changed via this menu.
If your system configuration includes the peristaltic pump, use this option to set the interval the peristaltic pump runs forward, drawing medium into the system, before the valves open to deliver samples.
Select option 5 , Prime Time. Enter a value for the priming time from 1 to 99 seconds and press ENTER . The Program Variables screen displays.
Note: At a minimum, the prime time should be long enough for medium to be seen dripping from the return cannulas. In general, the longer the tubing lengths, the longer the priming time required.
If your system configuration includes the syringe pump, use this option to set the amount of drawn medium necessary to fill the sampling lines of the entire system.
Select option 5 , Prime Volume. Enter a value for the priming volume and press
ENTER . The Program Variables screen displays.
Note: At a minimum the prime volume should be large enough for medium to be seen dripping from the return cannulas. In general, the longer the tubing lengths, the larger the prime volume required.
If your system configuration includes the peristaltic pump, use this option to set the interval the peristaltic pump runs in reverse to return the uncollected medium in each line back into the vessel from which it was drawn.
Select option 6 , Purge Time. Enter a value for the purge time from 1 to 99 seconds and press ENTER . The Program Variables screen displays.
Note: Purging also clears the sampling filters of particulate matter that may restrict successive samples. As with priming, the longer the tubing lengths, the longer the purge time required.
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Table 10 Program Variables Options (Apparatus 1 / 2)
Option
6 Purge Volume (syringe pump)
Function
If your system configuration includes the syringe pump, use this option to set a purge volume that ensures all stranded medium is properly expelled.
Select option 6 , Purge Volume. Enter a value for the purge volume that ensures at least two strokes of the syringe plunger and press ENTER . The Program Variables screen displays.
Note: The first stroke moves all medium from the sampling lines to the return lines.
The second stroke draws air into the sampling lines and purges the remaining medium from the return lines. As with priming, the longer the tubing lengths, the larger the purge volume required.
7 R-M (peristaltic pump) If your system configuration includes the peristaltic pump, select option 7 , R-M. The
Replacement Media Option screen displays. Select option 1 , Enable. Enter the desired percentage of replacement medium and press ENTER . Optionally, select option 2 , Disable. The Program Variables screen displays.
Note: If the RM option is not installed, select option 2 , Disable.
7 Filter Chg (syringe pump and filter changer)
If your system configuration includes the syringe pump and filter changer, select option 7 , Filter Chg. Enter the desired number of samples each filter should process before being discharged and press ENTER . The recommended value is 1. The
Program Variables screen displays.
Note: If your system configuration includes the syringe pump and filter changer, ensure the syringe pump option is enabled. From the Ready screen, press MENU .
Select option 3, Set Syringe Pump. Select option 1, Enable. Also press MENU > 0 >
P and select option 1 , Enable.
The replacement media option is not available with this system configuration. Ensure the RM option for the syringe pump is disabled using the hidden key function MENU
> 0 > R.
7 R-M (syringe pump)
If your system configuration includes the syringe pump but not the filter changer, select option 7 , R-M. The Replacement Media Option screen displays. Select option
1 , Enable, or option 2 , Disable. If the RM option is not installed, select option 2,
Disable. The Program Variables screen displays.
Note: If your system configuration includes the syringe pump, ensure the syringe pump option is enabled. From the Ready screen, press MENU . Select option 3 , Set
Syringe Pump. Select option 1 , Enable. Also press MENU > 0 > P and select option
1 , Enable.
If your system configuration includes the syringe pump but not the filter changer, use the hidden key function MENU > 0 > R to enable or disable the RM option, as appropriate. If the RM option is not installed, select option 2 , Disable.
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NOTE
NOTE
Program Option 2 - Sample Time Points
From the Program Variables screen, select option 2 , Sample Time
Points. The following screen displays:
SAMPLE TIME POINT: 000:02
ENTER hhh:mm UP TO 999:59 HOURS
PROG #: 1 ROW: 0 VOLUME: 5ml
TIME:01/01/12 09:37:14 ELAPSE:000:00:00
This screen accepts sampling times and saves them for execution when the program is run. Time points are requested in order of execution starting with collection Row 0 on the sample tray. The sample time is entered as hours and minutes. Once you enter a time, the row number increments and another sample time point is requested.
The following conventions are used with this screen:
• Review previously entered sample time points by pressing ENTER to scroll through the entries.
• After all desired sample time points have been entered, enter 000:00 for the last sample time point and press ENTER . If the maximum of ten sample time points are entered, you do not have to enter 000:00 for the last sample time point.
If a sample time point is required after the final spin (250 RPM), enter a maximum of nine sample time points. The firmware accounts for the final spin sample, but the tenth space must be left blank.
• To add additional sample time points, press ENTER until a new row time displays. Enter the additional sample time point.
• If more than ten sample time points are needed, use the link program
feature. See “Starting a 8000 Program” on page 66.
• To correct an entry, press BACKSPACE to go to the previous character position and re-enter the correct value.
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NOTE
• If a shorter sample time point than the previous one is entered for a given row, the row number will not increment. Times must increase from one row to the next.
• To shorten a saved program, enter 000:00 for the first sample time point you wish to discard. All successive time points are eliminated.
• Press ESC to save all new values and return to the Program Variables screen.
Sample time points should be at least five minutes apart to allow adequate time for priming and purging the sampling lines. If your system configuration includes the syringe pump and filter changer, ensure the sample time points are at least six minutes apart.
Starting a 8000 Program
1 From the Ready screen, press START PROG to start a program or modify a program already operating. The Enter Prog screen displays.
NOTE
If using baskets or rotating cylinders, ensure the drive unit of the dissolution apparatus is raised to its highest position before starting the
8000 program.
If using a dissolution apparatus with the 8000, you must enable external control on the dissolution apparatus and log out.
2 Enter a program number between 1 and 15 and press ENTER . The
Link Program Option screen displays.
***LINK PROGRAM OPTION***
1 RUN SINGLE PROG 2 LINK ANOTHER PROG
TIME: 01/01/12 15:02:30 ELAPSE: 000:00:04
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NOTE
3 To run a single program, select option 1 .
If more than ten rows are required to collect samples, linking a program allows you to run two programs with identical test parameters back to back. To link identical programs, link the program to itself.
To link another program to the one previously selected, select option 2 .
The following screen displays:
NOTE
LINK PROG #: (1-15):__
TIME: 01/01/12 15:02:30 ELAPSE: 000:00:04
Enter the program number to link to the current program and press
ENTER .
When running two programs, it is necessary to replace the full sample tray with an empty one between programs. Enable the program completion alarm (see “5 Prg Compl Alarm” under “Main Menu Option
1 Set Clock/Alarms” on 40) to alert the analyst when the first program completes and the second program begins.
4 The Select Start Mode screen displays. Options 1 - 3 start the collection program. Option 4 allows you to change or review the program before beginning.
***SELECT START MODE***
1 START NOW
3 REMOTE START
PROG#: 1 R-M: DISABLED
2 DELAYED START
4 MODIFY PROGRAM
VOL: 5 ml
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Following is a description of the Select Start Mode screen options:
Table 11 Select Start Mode Options (Apparatus 1 / 2)
Option
1 Start Now
2 Delayed Start
3 Remote Start
4 Modify Program
Function
Select option 1 , Start Now. The DISSO Options screen displays. See “DISSO
Options Screen” on page 69.
Select option 2 , Delayed Start. The DISSO Options screen displays. See “DISSO
Options Screen” on page 69.
This option is designed for use with the 8000 coupled with non-Agilent dissolution apparatus. Contact the Dissolution Systems Service Department for information about this function.
Select option 4 , Modify Program, to modify or review the selected program.
“Creating a Sample Program” on page 61
to review programming options. All program variable items can be viewed or changed.
Press ESC to return to the Select Start Mode screen.
Selecting option 4 again repeats the modify program operation.
When the program is verified or changed to the correct configuration, select the desired start mode (option 1 - 3) to start the program.
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DISSO Options Screen
***DISSO OPTIONS***
1 LOAD & RUN DISSO
3 RUN 8000 ONLY
2 RUN WO/ LOADING
4 SET DISSO
TIME: 01/01/12 08:09:51 ELAPSE: 000:00:00
Following is a description of the DISSO Options screen options:
Table 12 Disso Options (Apparatus 1 / 2)
Option
1 Load & Run
DISSO
2 Run WO/
Loading
Start Now Function
Use this option to upload all saved parameters to the dissolution apparatus and start the program. Select option 1 ,
Load & Run DISSO. The Select
DISSO Program Start Mode screen displays. See “Select DISSO Program
Start Mode Screen” on page 70.
Delayed Start Function
Use this option to program and run the
8000 without loading and overriding dissolution apparatus settings. Select option 2 , Run WO/ Loading. The
Select DISSO Program Start Mode screen displays. See “Select DISSO
Program Start Mode Screen” on page 70.
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Table 12 Disso Options (Apparatus 1 / 2)
Option
3 Run 8000
Only
4 Set DISSO
Start Now Function
Select option 3 , Run 8000 Only, to immediately start the 8000 only. The
8000 executes the program currently selected.
This option is used when other instrumentation, such as a dissolution apparatus, is connected to the 8000 through the START OUTPUT jack on the rear of the instrument.
Delayed Start Function
Select option 3 , Run 8000 Only, to start the 8000 only.
Enter the start time in 24-hour format and press ENTER .
Enter the start date in the appropriate format and press
ENTER . The DISSO Options screen displays.
If the delayed start alarm is enabled, enter the duration before the start of the program you would like the alarm to sound. To disable the alarm, see MENU > 0 > C under
“Hidden Key Functions” on page 41.
This option is used when other instrumentation, such as a dissolution apparatus, is connected to the 8000 through the
START OUTPUT jack on the rear of the instrument.
Use this option to modify or review the current program parameters. Select option 4 , Set DISSO. The Set DISSO
Parameters screen displays. See “Main
Menu Option 2 - Set DISSO” on page 53 for a description of the DISSO
Parameters screen options.
Select DISSO Program Start Mode Screen
***SELECT DISSO PROGRAM START MODE***
1 INSTANT 2 SET VESSEL TEMP AND START
TIME: 01/01/12 08:09:51 ELAPSE: 000:00:00
Following is a description of the Select DISSO Program Start Mode screen options:
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Table 13 Select Disso Program Start Mode Options (Apparatus 1 / 2)
Option
1 Instant
2 Set Vessel Temp And
Start
Start Now Function
Select option 1 , Instant, to start the dissolution apparatus instantly. To stop the dissolution apparatus, press
ESC . A confirmation screen displays. Press Y to stop the program. Press N to continue running the program.
Delayed Start Function
Select option 1 , Instant, to set the desired time to start the dissolution apparatus.
Enter the start time in 24-hour format and press ENTER .
Enter the start date in the appropriate format and press
ENTER . The screen displays the program information, including the entered start time and date.
If the delayed start alarm is enabled, enter the duration before the start of the program you would like the alarm to sound. To disable the alarm, see MENU > 0 > C under
“Hidden Key Functions” on page 41.
Press ESC to cancel the delayed start.
Select option 2 , Set Vessel
Temp And Start, to set the temperature the vessels have to reach to initiate the test. Enter the desired vessel temperature and press ENTER . The program starts once the set vessel temperature is reached.
Select option 2 , Set Vessel Temp And Start, to set the temperature the vessels have to reach to initiate the test.
Enter the desired vessel temperature and press ENTER .
Enter the start time in 24-hour format and press ENTER .
Enter the start date in the appropriate format and press
ENTER . The screen displays the program information, including the entered start time and date.
If the delayed start alarm is enabled, enter the duration before the start of the program you would like the alarm to sound. To disable the alarm, see MENU > 0 > C under
“Hidden Key Functions” on page 41.
Press ESC to cancel the delayed start.
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Operating the 8000 with Apparatus 3 / 7
8000 Keypad Options
The keypad on the 8000 is similar to a personal computer keyboard. For example, it has a SPACE key, an ENTER key, and a SHIFT key. These keys function exactly like their counterparts on a personal computer or
typewriter keyboard. See Figure 15, “8000 Keypad,” below. A
discussion about each of the main function keys follows.
Figure 15 8000 Keypad
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The front panel options include the following:
Table 14 Front Panel Options (Apparatus 3 / 7)
Option
ESC
MENU
CAL
PROG
START PROG
MANUAL SAMPLE
OPEN VALVES
PUMP OFF
PUMP FWD
PUMP REV
Function
Press ESC to stop execution of a running program or calibration routine. Press
ESC to move back one level to the previous menu or selection when moving through the multi-level menu.
Be very careful when using ESC while a program is running. If a program or calibration is running and ESC is pressed, a prompt appears on the first line of the display asking if you want to stop the running program. The options are Y or N.
Press Y to stop the running program. Press N to continue the running program.
Use this option to set the clock, alarms, Apparatus 3 / Apparatus 7 parameters, start output delay / syringe pump parameters, manual prime time / volume, manual purge time / volume as well as to remotely control the Apparatus 3 / 7 and manually control the 8000. See
“8000 with Apparatus 3 / 7 - Menu Options” on page 74.
Press PRINT to record the batch information, print the dip and drain times, enable or disable the Report Center Printer, select a local or remote printer, set the
communication port identification number, and set the baud rate. See “Printer
Operation and Communications” on page 103.
Use this option to calibrate the 8000 to ensure that sample volumes are accurately and precisely taken. See
“Volumetric Calibration (Peristaltic Pump only)” on page 97.
Use this option to set program parameters. See “Creating a Sample Program” on page 61.
Use this option to start a program. See “Starting a Program” on page 91.
Use this option to collect a sample from the dissolution vessels on command
without a program. See “Manual Sample” on page 95.
Press OPEN VALVES to open all valves simultaneously. This function keeps the valves open as long as the key on the keypad is pressed. To change the valve configuration, press MENU > 0 > 0 and select the appropriate configuration.
PUMP OFF, PUMP FWD, and PUMP REV work exclusively with the Agilent peristaltic pump. When the syringe pump is connected, they are disabled.
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Table 14 Front Panel Options (Apparatus 3 / 7)
Option
CLEAN SYTEM
LOCK KBRD
Function
Use this option to keep the valves, needles, and fluid lines clean. See
on page 106 or “Clean System Function with
Press LOCK KBRD to lock the keypad. Press LOCK KBRD again to unlock the keypad.
8000 with Apparatus 3 / 7 - Menu Options
Main Menu
From the Ready screen, press MENU . The Main Menu displays.
Options 3, 4, and 5 change depending on your system configuration.
When your system configuration includes the peristaltic pump, the following Main Menu displays:
***MAIN MENU***
1 SET CLOCK/ALARMS
2 SET APP 3/7
3 START OUTPUT DELAY
4 MANUAL PRIME TIME
5 MANUAL PURGE TIME
6 CONTROL APP 3/7
7 MANUAL OPERATION
When your system configuration includes the syringe pump with or without the filter changer, the following Main Menu displays:
***MAIN MENU***
1 SET CLOCK/ALARMS
2 SET APP 3/7
3 SET SYRINGE PUMP
4 MANUAL PRIME VOL.
5 MANUAL PURGE VOL.
6 CONTROL APP 3/7
7 MANUAL OPERATION
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Following is a description of the Main Menu screen options:
Table 15 Main Menu Options (Apparatus 3 / 7)
Option
1
2
3
Set Clock/Alarms
Set APP 3/7
Start Output Delay
(peristaltic pump)
Function
Use this option to set the clock and alarms. If your system configuration includes the syringe pump, the start output delay option also displays on this screen. See “Main
Menu Option 1 - Set Clock/Alarms” on page 77.
Use this option to set the Apparatus 3 / 7 parameters. See “Main Menu Option 2 - Set
DISSO” on page 53.
If your system configuration includes the peristaltic pump, use this option to set the delay from the start of a program until the TTL signal is sent from the START
OUTPUT jack on the 8000 rear panel.
Select option 3 , Start Output Delay. Enter a delay length of up to 99:59 minutes for the 8000 TTL output and press ENTER . The Main Menu displays.
Note: This output signal can be used to start other instrumentation, such as an
Apparatus 3 / 7, after the program on the 8000 is started. The default delay is 00:00, indicating that the signal will be sent at the moment a program starts. For example, a value of 10:00 delays the signal for ten minutes after the program starts. Set the value to 00:00 to eliminate the delay feature.
When the syringe pump is enabled, Start Output Delay displays under the Set Clock and Alarms screen as option 6. See “6 Start Output Delay” on page 52.
3 Set Syringe Pump (syringe pump)
If your system configuration includes the syringe pump, use this option to set the syringe pump parameters. See “Main Menu Option 3 - Set Syringe Pump” on page 80.
4 Manual Prime Time
(peristaltic pump)
If your system configuration includes the peristaltic pump, use this option to set the interval the peristaltic pump runs forward, drawing medium into the system, before the valves open to deliver samples.
Select option 4 , Manual Prime Time. Enter a value for the priming time from 1 to 99 seconds and press ENTER . The Main Menu displays.
Note: At a minimum, the manual prime time should be long enough for medium to be seen dripping from the return cannulas. In general, the longer the tubing lengths, the longer the priming time required. This value is not used by sampling programs, which have their own individual priming time values. It is used only with the
MANUAL SAMPLE, CAL, and CLEAN SYSTEM keys on the 8000.
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Table 15 Main Menu Options (Apparatus 3 / 7)
Option
4 Manual Prime Vol.
(syringe pump)
5 Manual Purge Time
(peristaltic pump)
5 Manual Purge Vol.
(syringe pump)
6 Control APP 3/7
7 Manual Operation
Function
If your system configuration includes the syringe pump, use this option to set the amount of drawn medium necessary to fill the sampling lines of the entire system.
Select option 4 , Manual Prime Vol. Enter a value for the priming volume and press
ENTER . The Main Menu displays.
Note: At a minimum, the manual prime volume should be large enough for medium to be seen dripping from the return cannulas. In general, the longer the tubing lengths, the larger the priming volume required. This value is not used by sampling programs which have their own individual priming volume values. It is used only with the MANUAL SAMPLE and CLEAN SYSTEM keys on the 8000.
If your system configuration includes the peristaltic pump, use this option to set the interval the peristaltic pump runs in reverse to return the uncollected medium in each line back into the vessel from which it was drawn.
Select option 5 , Manual Purge Time. Enter a value for the manual purge time from 1 to 99 seconds and press ENTER . The Main Menu displays.
Note: Purging also clears the sampling filters of particulate matter that may restrict successive samples. As with priming, the longer the tubing lengths, the longer the purge time required. This value is not used by sampling programs, which have their own individual purging time values. It is used only with the MANUAL SAMPLE,
CAL, and CLEAN SYSTEM keys on the 8000.
If your system configuration includes the syringe pump, use this option to set the a purge volume that ensures all stranded medium is properly expelled.
Select option 5 , Manual Purge Vol. Enter a value for the purge volume that ensures at least two strokes of the plunger and press ENTER . The Main Menu displays.
Note: The first stroke moves all medium from the sampling lines to the return lines.
The second stroke draws air into the sampling lines and purges the remaining medium from the return lines. As with priming, the longer the tubing lengths, the larger the purge volume required. This value is not used by sampling programs which have their own individual purge volume values. It is used only with the
MANUAL SAMPLE and CLEAN SYSTEM keys on the 8000.
Use this option to remotely control the Apparatus 3 / 7 from the 8000. See “Main
Menu Option 6 - Control APP 3/7” on page 82.
Use this option to manually control the 8000. See “Main Menu Option 7 - Manual
Operation” on page 83.
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Main Menu Option 1 - Set Clock/Alarms
From the Main Menu, select option 1 , Set Clock/Alarms. The Set Clock and Alarms screen displays.
1 SET CLOCK
***SET CLOCK AND ALARMS***
2 TIMER ALARM
3 ROW COMPL ALARM
5 PRG COMPL ALARM
4 CALIB. COMPL ALARM
6 START OUTPUT DELAY
Following is a description of the Set Clock and Alarms screen options:
Table 16 Set Clock and Alarms Options
Option
1 Set Clock
2 Timer Alarm
3 Row Compl Alarm
Function
Select option 1 , Set Clock. Enter the date in the appropriate format and press
ENTER . The time displays in 24-hour format. If the time is correct, press ENTER or
ESC to return to the Main Menu. If the time is incorrect, enter the correct time in
24-hour format and press ENTER . The Set Clock and Alarms screen displays.
Use this option to set the elapsed timer alarm. The elapsed timer alarm is a simple timer you can use to time any interval from 1 minute to 99 hours. The alarm sounds when the time has expired. Once turned off, the timer does not operate again until you reset it.
Select option 2 , Timer Alarm. Enter a duration in hh:mm format and press ENTER .
The Set Clock and Alarms screen displays.
Press any key to silence the alarm after it sounds.
Use this option to set an alarm to indicate the completion of each sample time point.
This alarm is useful when the samples must be checked or removed for immediate analysis.
Select option 3 , Row Compl Alarm. Select option 1 to enable or option 2 to disable the alarm. The Set Clock and Alarms screen displays.
Press any key to silence the alarm after it sounds.
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Table 16 Set Clock and Alarms Options
Option
4 Calib. Compl Alarm
5 Prg Compl Alarm
6 Start Output Delay
Function
Use this option to set an alarm to sound when autocalibration is complete and all lines are purged.
Select option 4 , Calib. Compl Alarm. Select option 1 to enable or option 2 to disable the alarm. The Set Clock and Alarms screen displays.
Press any key to silence the alarm after it sounds.
Note: This option is only available if your system configuration includes the peristaltic pump.
Use this option to set an alarm to sound at the end of the program. It is a useful reminder that the program is complete.
Select option 5 , Prg Compl Alarm. Select option 1 to enable or option 2 to disable the alarm. The Set Clock and Alarms screen displays.
Press any key to silence the alarm after it sounds.
Note: Option 6 displays only when the syringe pump is enabled.
If the syringe pump is enabled, use this option to set the delay from the start of a program until the TTL signal is sent from the START OUTPUT jack on the 8000 rear panel.
Select option 6 , Start Output Delay. The Start Output Delay screen displays. Enter a delay length of up to 99:59 minutes for the 8000 TTL output and press ENTER . The
Set Clock and Alarms screen displays.
Note: This output signal can be used to start other instrumentation, such as an
Apparatus 3 / 7, after the program on the 8000 is started. The default delay is 00:00, indicating that the signal will be sent at the moment a program starts. For example, a value of 10:00 delays the signal for ten minutes after the program starts. Set the value to 00:00 to eliminate the delay feature.
When the syringe pump is disabled, this option displays under the Main Menu. See “3
Start Output Delay (peristaltic pump)” on page 49.
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Main Menu Option 2 - Set APP 3/7
From the Main Menu, select option 2 , Set APP 3/7. Enter a program number and press ENTER . The Set APP 3/7 Parameters screen displays.
***SET APP 3/7 PARAMETERS***
1 DIPPING SPEED (DPM) 2 SAMPLES PER ROW
3 HOLD DIP TIME
5 COM PORT ID
4 SET BATH TEMP
6 PRINT FREQUENCY
Following is a description of the Set APP 3/7 Parameters screen options:
Table 17 Set App 3/7 Parameters Options (Apparatus 3 / 7)
Option
2 Samples Per Row
Function
1 Dipping Speed (DPM) Select option 1 , Dipping Speed (DPM). Enter the desired DPM and press
ENTER . The Set APP 3/7 Parameters screen displays.
Select option 2 , Samples Per Row. Enter the desired number of samples to be taken from each row and press ENTER . The Set APP 3/7 Parameters screen displays.
3 Hold Dip Time
4 Set Bath Temp
Select option 3 , Hold Dip Time. Enter a hold dip time and press ENTER to advance to each remaining row. After a hold dip time is entered for the final row, the Set APP 3/7 Parameters screen displays.
Select option 4 , Set Bath Temp. Enter the desired water bath temperature and press ENTER . The Set APP 3/7 Parameters screen displays.
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Table 17 Set App 3/7 Parameters Options (Apparatus 3 / 7)
Option
5 Com Port ID
6 Print Frequency
Function
Select option 5 , Com Port ID, to change the communication port identification number. Your system was shipped with the communication port identification number set to 01. You can set the identification number to any number between 1 and 99. The identification number is used when multiple units are connected in series so that the controlling or master 8000 can start individual units.
Enter a communication port identification number and press ENTER . The Set
APP 3/7 Parameters screen displays.
The Apparatus 3 / 7 status prints at each sample time point. Select option 6 , Print
Frequency, to set additional times to print the Apparatus 3 / 7 status between sample time points. This option should be reserved for use when there is more than 30 minutes between sample time points.
Enter a print frequency and press ENTER . The Set APP 3/7 Parameters screen displays.
The print frequency timer resets after each sample time point.
Note: The status does not print if the specified print frequency time falls within three minutes of the sample time point; the sample time point takes precedence.
NOTE
Once all values have been entered, press ESC . A confirmation screen displays. Press Y to send the newly entered parameters to the Apparatus
3 / 7 and override the existing settings.
Main Menu Option 3 - Set Syringe Pump
This section is applicable only to systems including a syringe pump.
Ensure the syringe pump is enabled prior to setting the syringe pump
parameters (see MENU > 0 > P under “Hidden Key Functions” on page 41
.
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From the Main Menu, select option 3 , Set Syringe Pump. Select option
2 to disable the syringe pump or select option 1 to enable the syringe pump. Once enabled, the Set Syringe Pump Parameters screen displays.
***SET SYRINGE PUMP PARAMETERS***
1 SYRINGE SIZE 2 PLUNGER SPEED
3 PRIME LOSS VOLUME 4 ASPIRT. DWELL TIME
Following is a description of the Set Syringe Pump Parameters screen options:
Table 18 Set Syringe Pump Parameters Options (Apparatus 3 / 7)
Option
1 Syringe Size
2 Plunger Speed
3 Prime Loss Volume
Function
Use this option to set the syringe size.
Select option 1 , Syringe Size. Select the syringe size that corresponds with the
Kloehn syringes loaded into the syringe pump. The Set Syringe Pump Parameters screen displays.
Use this option to configure the syringe pump plunger to operate at a specific speed.
Select option 2 , Plunger Speed. Enter a plunger speed between 100 and 1500 motor steps per second (sps) and press ENTER . The recommended value is 500. The Set
Syringe Pump Parameters screen displays.
Use this option to configure the prime loss volume. Prime loss volume compensates for the volume of medium used to fill the tubing between the sampling cannulas and sampling valves during the priming cycle. When sampling, an amount of sample equal to the prime loss volume flows past the valves in the 8000 before the valves open to collect sample. The program also restricts this volume to be less than or equal to the syringe size volume.
Select option 3 , Prime Loss Volume. Enter a prime loss volume based on the sample tubing length, inner diameter, and system dead volumes and press ENTER . The Set
Syringe Pump Parameters screen displays.
If the tubing length between the sampling cannulas and sampling valves is changed, adjust the prime loss volume accordingly.
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Table 18 Set Syringe Pump Parameters Options (Apparatus 3 / 7)
Option
4 Aspirt. Dwell Time
5 Full Stroke (hidden option)
6 Pump Current (hidden option)
Function
Use this option to configure the aspiration dwell time. Aspiration dwell time is the time the syringe plunger pauses at the bottom of a stroke to allow the medium being drawn to overcome the vacuum generated by the drawn plunger.
Select option 4 , Aspirt. Dwell Time. Enter an aspiration dwell time between 1 and 10 seconds and press ENTER . The recommended time is 10 seconds. The Set Syringe
Pump Parameters screen displays.
Use this option to configure the number of steps the stepper motor makes to draw the syringe plunger its maximum stroke length.
Select option 5 , Full Stroke. Enter a full stroke value between 0 and 19999 and press
ENTER . The recommended value is 19300. The Set Syringe Pump Parameters screen displays.
If the volume dispensed by the syringe is outside of the required specifications based on a 10 mL syringe size, the full stroke value can be adjusted using the following calculation and solving for x: target volume (mL) = avg volume dispensed (mL)
x 19300
Use this option to configure the pump current. The use of more viscous media requires a higher pump current.
Select option 6 , Pump Current. Enter a pump current between 0 and 8 and press
ENTER . The recommended value is 4. The Set Syringe Pump Parameters screen displays.
Main Menu Option 6 - Control APP 3/7
From the Main Menu, select option 6 , Control APP 3/7. The APP 3/7
Control/Monitor screen displays.
***APP 3/7 CONTROL/MONITOR***
1 DIP 2 STOP 3 GO TO ROW 4 HOME
SET RPM: 50 ACTUAL RPM: 000.0
SET BATH TEMP: 37.5
ACTUAL TEMP: 37.2
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The following options are commands communicated from the 8000 to the Apparatus 3 / Apparatus 7:
Table 19 App 3/7 Control/Monitor Options
Option
1 Dip
2 Stop
3 Go To Row
4 Home
Function
Select option 1 , Dip, to begin dipping.
Select option 2 , Stop, to stop dipping and raise the cannulas.
Select option 3 , Go To Row, to move the drive unit to a specific row. Enter a row number and press ENTER . The Apparatus 3 / 7 drive unit moves to the indicated row.
Select option 4 , Home, to move the drive unit to the home position.
Press ESC to return to the Main Menu.
Main Menu Option 7 - Manual Operation
From the Main Menu, select option 7 , Manual Operation.
The following Manual Operations screen displays when your system configuration includes the peristaltic pump:
***MANUAL OPERATIONS***
1 LIFT VALVES 2 LOWER VALVES
3 OPEN/CLOSE VALVES
5 TURN ON (OFF) R-M PUMP
4 GOTO ROWS
The following Manual Operations screen displays when your system configuration includes the syringe pump with or without the filter changer:
***MANUAL OPERATIONS***
1 LIFT VALVES
3 OPEN/CLOSE VALVES
5 TURN ON (OFF) R-M PUMP
2 LOWER VALVES
4 GOTO ROWS
6 SYRINGE/FILTER
All functions execute immediately without pressing ENTER. Press ESC to return to the Main Menu.
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Following is a description of the Manual Operations screen options:
Table 20 Manual Operations (Apparatus 3 / 7)
Option
1 Lift Valves
2 Lower Valves
3 Open/Close Valves
4 Goto Rows
5 Turn On (Off) R-M
Pump
6 Syringe/Filter (syringe pump only)
Function
Select option 1 , Lift Valves, to raise the valve needles if lowered.
Select option 2 , Lower Valves, to lower the valve needles if raised.
Select option 3 , Open/Close Valves, to toggle the valves open and closed.
Select option 4 , Goto Rows, to move the 8000 dispensing arm to a specific row.
Enter a row number. The dispensing arm moves to the indicated row and stops.
If the moving alert is enabled, the alarm sounds when the dispensing arm
moves. To disable the alert, see MENU > 0 > B under “Hidden Key
Ensure TURN ON R M PUMP displays as option 5. This indicates the replacement media option is disabled. If TURN OFF R M PUMP displays, select option 5 . TURN
ON R M PUMP displays.
Note: The replacement media option must be disabled or the instrument will not operate properly.
Use this option to manually control the syringe pump and filter changer as applicable. See “Manual Operation Option 6 - Syringe/Filter” below.
Manual Operation Option 6 Syringe/Filter
From the Manual Operations screen, select option 6 , Syringe/Filter. The
Operate Syringe/ Filter Changer screen displays.
***OPERATE SYRINGE/FILTER CHANGER***
1 VALVE TO INPUT
3 SYRINGE FILL
5 FILTER CHANGER
2 VALVE TO OUTPUT
4 SYRINGE DISPENSE
6 SYRINGE PARAMETERS
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Following is a description of the Operate Syringe/Filter Changer screen options:
Table 21 Operate Syringe/Filter Changer Options
Option
1 Valve to Input
2 Valve to Output
3 Syringe Fill
4 Syringe Dispense
Function
The syringe pump contains a valve to control the direction of the flow of medium.
Use this option to move the valve to the input position to manually input or fill the syringe with medium.
Select option 1 , Valve to Input, to move the valve to the input position. The valve emits a loud click when it moves. If you hear no sound, the valve is already in the input position.
The syringe pump contains a valve to control the direction of the flow of medium.
Use this option to move the valve to the output position to manually output or purge the syringe of medium.
Select option 2 , Valve to Output, to move the valve to the output position. The valve emits a loud click when it moves. If you hear no sound, the valve is already in the output position.
Use this option to draw a specific amount of medium into the syringes. Ensure the valve is in the input position before drawing the medium (see “1 Valve to Input” above).
Select option 3 , Syringe Fill. Enter the desired percentage of each syringe to fill and press ENTER .
The syringes draw the specified amount of medium.
Use this option to dispense medium from the syringes.
Select option 4 , Syringe Dispense. The syringes dispense the previously drawn medium (see “3 Syringe Fill” above).
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Table 21 Operate Syringe/Filter Changer Options
Option
5 Filter Changer
6 Syringe Parameters
Function
Use this option to manually operate the filter changer.
Select option 5 , Filter Changer. The Filter Change Manual Control screen displays.
To open the filter clamp, select option 1 , Open Clamp.
To close the filter clamp, select option 2 , Close Clamp.
To change the filters, select option 3 , Change Filters. Enter the number of filters to change and press ENTER . The specified number of filters are expelled and replaced.
Press ESC to return to the Operate Syringe/Filter Changer screen.
Use this option to configure the syringe pump. Select option 6 , Syringe Parameters.
The Set Syringe Pump Parameters screen displays. For a description of the screen options, see “Main Menu Option 3 - Set Syringe Pump” on page 55.
Note: The syringe pump parameters are global. These parameters can be entered under option 3 of the Main Menu or option 6 of the Operate Syringe/Filter Changer screen.
Creating a Sample Program
NOTE
Programs are not write-protected. Check with other users before proceeding.
NOTE
1 From the Ready screen, press PROG to program the 8000.
2 Enter a program number and press ENTER . The Program Variables screen displays, as shown below.
The 8000 can store up to 15 programs in non-volatile memory. Valid program numbers are 1 to 15. If 0 (zero) or a number larger than 15 is entered, the system continues to prompt you until a valid number is entered.
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NOTE
The following Program Variables screen displays when your system configuration includes the peristaltic pump:
***PROGRAM VARIABLES***
1 SET APP 3/7 PARAMETERS
2 SAMPLE TIME POINTS
3 SAMPLE VOLUME
4 HEADERS
5 PRIME TIME
6 PURGE TIME
7 R-M
The following Program Variables screen displays when your system configuration includes the syringe pump with or without the filter changer:
***PROGRAM VARIABLES***
1 SET APP 3/7 PARAMETERS
2 SAMPLE TIME POINTS
3 SAMPLE VOLUME
4 HEADERS
5 PRIME VOLUME
6 PURGE VOLUME
7 FILTER CHG or R-M
Option 7 displays as Filter Chg when your system configuration includes the syringe pump and filter changer. See “7 Filter Chg (syringe pump and filter changer)” on page 51.
If your system configuration includes the syringe pump but not a filter changer, option 7 displays as R-M. See “7 R-M (syringe pump)” on page 51.
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Following is a description of the Program Variables screen options:
Table 22 Program Variables Options (Apparatus 3 / 7)
Option Function
1 Set APP 3/7 Parameters Use this option to set the Apparatus 3 / 7 parameters. Select option 1 , Set APP 3/7
Parameters. The Set APP 3/7 Parameters screen displays. See “Main Menu Option 2 -
Set DISSO” on page 53 for a description of the Set APP 3/7 Parameters screen options.
2 Sample Time Points Use this option to enter the sample time points. See “Program Option 2 - Sample Time
Points” on page 90.
3 Sample Volume
4 Headers
Select option 3 , Sample Volume. Enter the sample volume in milliliters and press
ENTER . The Program Variables screen displays. You can enter the volume with up to three digits (for example 13.5) so that fractional milliliters can be collected. Verify the collection tubes can hold the requested volume.
Note: The maximum volume accepted is 14 mL.
Select option 4 , Headers. The Batch # Selections screen displays. For each option, enter the desired information and press ENTER . The Program Variables screen displays.
Note: The Batch # Selections screen displays options for the report header information.
The print field accepts ten characters. Characters can be letters, numbers, special characters, or spaces. The information entered for each selection prints in the header of each printout. The entries made from this menu are attached to the active program.
Since there are 15 programs, there can be a different set of entries for each. All are stored in the battery-protected memory and remain until changed via this menu.
5 Prime Time (peristaltic pump)
5 Prime Volume (syringe pump)
If your system configuration includes the peristaltic pump, use this option to set the interval the peristaltic pump runs forward, drawing medium into the system, before the valves open to deliver samples.
Select option 5 , Prime Time. Enter a value for the priming time from 1 to 99 seconds and press ENTER . The Program Variables screen displays.
Note: At a minimum, the prime time should be long enough for medium to be seen dripping from the return cannulas. In general, the longer the tubing lengths, the longer the priming time required.
If your system configuration includes the syringe pump, use this option to set the amount of drawn medium necessary to fill the sampling lines of the entire system.
Select option 5 , Prime Volume. Enter a value for the priming volume and press
ENTER . The Program Variables screen displays.
Note: At a minimum, the prime volume should be large enough for medium to be seen dripping from the return cannulas. In general, the longer the tubing lengths, the larger the prime volume required.
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Table 22 Program Variables Options (Apparatus 3 / 7)
Option
6 Purge Time (peristaltic pump)
Function
If your system configuration includes the peristaltic pump, use this option to set the interval the peristaltic pump runs in reverse to return the uncollected medium in each line back into the vessel from which it was drawn.
Select option 6 , Purge Time. Enter a value for the purge time from 1 to 99 seconds and press ENTER . The Program Variables screen displays.
Note: Purging also clears the sampling filters of particulate matter that may restrict successive samples. As with priming, the longer the tubing lengths, the longer the purge time required.
6 Purge Volume (syringe pump)
If your system configuration includes the syringe pump, use this option to set a purge volume that ensures all stranded medium is properly expelled.
Select option 6 , Purge Volume. Enter a value for the purge volume that ensures at least two strokes of the syringe plunger and press ENTER . The Program Variables screen displays.
Note: The first stroke moves all medium from the sampling lines to the return lines.
The second stroke draws air into the sampling lines and purges the remaining medium from the return lines. As with priming, the longer the tubing lengths, the larger the purge volume required.
7 R-M (peristaltic pump) If your system configuration includes the peristaltic pump, select option 7 , R-M. The
Replacement Media Option screen displays. Select option 2 , Disable. The Program
Variables screen displays.
Note: The replacement media option is not available for use with the Apparatus 3 / 7.
The replacement media option must be disabled or the instrument will not operate properly.
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Table 22 Program Variables Options (Apparatus 3 / 7)
Option
7 Filter Chg (syringe pump and filter changer)
7 R-M (syringe pump)
Function
If your system configuration includes the syringe pump and filter changer, select option
7 , Filter Chg. Enter the desired number of samples each filter should process before being discharged and press ENTER . The recommended value is 1. The Program
Variables screen displays.
Note: If your system configuration includes the syringe pump and filter changer, ensure the syringe pump option is enabled. From the Ready screen, press MENU . Select option 3 , Set Syringe Pump. Select option 1 , Enable. Also press MENU > 0 > P and select option 1, Enable.
The replacement media option is not available with this system configuration. Ensure the RM option for the syringe pump is disabled using the hidden key function MENU >
0 > R.
If your system configuration includes the syringe pump but not the filter changer, select option 7 , R-M. The Replacement Media Option screen displays. Select option 2 ,
Disable. The Program Variables screen displays.
Note: The replacement media option is not available for use with the Apparatus 3 / 7.
The replacement media option must be disabled or the instrument will not operate properly.
Program Option 2 - Sample Time Points
From the Program Variables screen, select option 2 , Sample Time
Points. The following screen displays:
SAMPLE TIME POINT: 000:02
ENTER hhh:mm UP TO 999:59 HOURS
PROG #: 1 ROW: 0 VOLUME: 5ml
TIME:01/01/12 09:37:14 ELAPSE:000:00:00
NOTE
This screen accepts sampling times and saves them for execution when the program is run. Time points are requested in order of execution starting with collection row number 0 on the sample tray. The sample time is entered as hours and minutes. Once you enter a time, the row number increments and another sample time point is requested.
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NOTE
The following conventions are used with this screen:
• Review previously entered sample time points by pressing ENTER to scroll through the entries.
• After all desired sample time points have been entered, enter 000:00 for the last sample time point and press ENTER . If the maximum of ten sample time points are entered, you do not have to enter 000:00 for the last sample time point.
• To add additional sample time points, press ENTER until a new row time displays. Enter the additional sample time point.
• If more than ten sample time points are needed, use the link program
feature. See “Starting a Program” on page 91.
• To correct an entry, press BACKSPACE to go to the previous character position and re-enter the correct value.
• If a shorter sample time point than the previous one is entered for a given row, the row number will not increment. Times must increase from one row to the next.
• To shorten a saved program, enter 000:00 for the first sample time point you wish to discard. All successive time points are eliminated.
• Press ESC to save all new values and return to the Program Variables screen.
Sample time points should be at least five minutes apart to allow adequate time for priming and purging the sampling lines. If your system configuration includes the syringe pump and filter changer, ensure the sample time points are at least six minutes apart.
Starting a Program
1 From the Ready screen, press START PROG to start a program or modify a program already operating.
2 Enter a program number between 1 and 15 and press ENTER . The
Link Program Option screen displays.
***LINK PROGRAM OPTION***
1 RUN SINGLE PROG 2 LINK ANOTHER PROG
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NOTE
TIME: 01/01/12 15:02:30 ELAPSE: 000:00:04
3 To run a single program, select option 1 .
If more than ten rows are required to collect samples, linking a program allows you to run two programs with identical test parameters back to back. To link identical programs, link the program to itself.
To link another program to the one previously selected, select option 2 .
The following screen displays:
NOTE
LINK PROG #: (1-15):__
TIME: 01/01/12 15:02:30 ELAPSE: 000:00:04
Enter the program number to link to the current program and press
ENTER .
When running two programs, it is necessary to replace the full sample tray with an empty one between programs. Enable the program completion alarm (see “5 PRG COMPL ALARM” on page 51) to alert the analyst when the first program completes and the second program begins.
4 The Select Start Mode screen displays. Options 1 - 3 start the collection program. Option 4 allows you to change or review the program before beginning.
***SELECT START MODE***
1 START NOW
3 REMOTE START
PROG#: 1 R-M: DISABLED
2 DELAYED START
4 MODIFY PROGRAM
VOL: 5 ml
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Following is a description of the Select Start Mode screen options:
Table 23 Select Start Mode Options (Apparatus 3 / 7)
Option
1 Start Now
2 Delayed Start
3 Remote Start
4 Modify Program
Function
Select option 1 , Start Now. The APP 3/7 Options screen displays.
Select option 2 , Delayed Start. The APP 3/7 Options screen displays.
This option is designed for use with the 8000 coupled with non-Agilent instruments. Contact the Dissolution Systems Service Department for information about this function.
Select option 4 , Modify Program, to modify or review the selected program.
“Creating a Sample Program” on page 61 to review programming options. All
program variable items can be viewed or changed.
Press ESC to return to the Select Start Mode screen.
Selecting option 4 again repeats the modify program operation.
When the program is verified or changed to the correct settings, select the desired start mode (option 1 - 3) to start the program.
APP 3/7 Options Screen
***APP 3/7 OPTIONS***
1 LOAD & RUN APP 3/7 2 RUN WO/ LOADING
3 RUN 8000 ONLY 4 SET APP 3/7
TIME: 01/01/12 08:09:51 ELAPSE: 000:00:00
Following is a description of the APP 3/7 Options screen options:
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Table 24 App 3/7 Options
Option
1 Load &
Run APP 3/7
2 Run WO/
Loading
3
Only
4
Run 8000
Set APP
3/7
Start Now Function Delayed Start Function
Select option 1 , Load & Run APP
3/7, to upload all saved parameters to the Apparatus 3 / 7 and start the program.
Select option 1 , Load & Run APP 3/7, to upload all saved parameters to the Apparatus 3 / 7.
Enter the start time in 24-hour format and press ENTER . Enter the start date and press ENTER .
If the delayed start alarm is enabled, enter the duration before the start of the program you would like the alarm to sound. To disable the alarm, see MENU > 0 > C under
“Hidden Key Functions” on page 41.
At any time prior to the scheduled start of the program, press ESC to cancel the delayed start.
Select option 2 , Run WO/
Loading, to start the program and run the 8000 without loading and overriding the Apparatus 3 / 7 settings.
Select option 3 , Run 8000 Only, to immediately start the 8000 only.
The 8000 executes the program currently selected.
This option is used when other instrumentation, such as an
Apparatus 3 / 7, is connected to the 8000 through the START
OUTPUT jack on the rear of the instrument.
Select option 2 , Run WO/ Loading, to run the 8000 without loading and overriding the Apparatus 3 / 7 settings.
Enter the start time in 24-hour format and press ENTER . Enter the start date and press ENTER .
If the delayed start alarm is enabled, enter the duration before the start of the program you would like the alarm to sound. To disable the alarm, see MENU > 0 > C under
“Hidden Key Functions” on page 41.
At any time prior to the scheduled start of the program, press ESC to cancel the delayed start.
Select option 3 , Run 8000 Only, to start the 8000 only.
Enter the start time in 24-hour format and press ENTER . Enter the start date and press ENTER . Once the start date and time are reached, the 8000 executes the program currently selected.
If the delayed start alarm is enabled, enter the duration before the start of the program you would like the alarm to sound. To disable the alarm, see MENU > 0 > C under
“Hidden Key Functions” on page 41.
At any time prior to the scheduled start of the program, press ESC to cancel the delayed start.
Use this option to modify or review the current program parameters. Select option 4 , Set APP 3/7. The
Set APP 3/7 Parameters screen displays. See “Main Menu Option 2 - Set APP 3/7” on page 79 for a description of the Set APP 3/7 Parameters screen options.
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Manual Sample
From the Ready screen, press MANUAL SAMPLE to collect a sample from the dissolution apparatus on command without a program. These options are only for manual sampling and have nothing to do with the stored program parameters for purge volume, prime volume, and so on.
When your system configuration includes the peristaltic pump, the following Manual Sampling screen displays:
***MANUAL SAMPLING***
1 START 2 SET ROW #
4 PRIME TIME 5 PURGE TIME
3 SET VOLUME
6 R-M OPTION
ROW 3 VOLUME 5ml PRIME 60 PURGE 60
When your system configuration includes the syringe pump with or without the filter changer, the following Manual Sampling screen displays:
***MANUAL SAMPLING***
1 START 2 SET ROW # 3 SET VOLUME
4 PRIME VOL. 5 PURGE VOL.
6 R-M OPTION
ROW 3 VOLUME 5ml PRIME 10 PURGE 20
Following is a description of the Manual Sampling screen options:
Table 25 Manual Sampling Options
Option
1 Start
2
Set Row #
Function
Select option 1 , Start, to start the manual sampling procedure using the values you enter for options 2 through 6 on the Manual Sampling screen. The display screen details each step of the manual collection as it takes place.
Note: Select this option last.
Select option 2 , Set Row #. Enter the row number between 0 and 9 where you want the samples deposited in the sample tray and press ENTER . The Manual Sampling screen displays.
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Table 25 Manual Sampling Options
Option
3 Set Volume
4 Prime Time
(peristaltic pump)
4 Prime Vol (syringe pump)
5 Purge Time
(peristaltic pump)
5 Purge Vol (syringe pump)
6 R-M Option
Function
Select option 3 , Set Volume. Enter a sample volume between 0 and 14 mL and press
ENTER . The Manual Sampling screen displays.
Note: The volume can contain a decimal point so that fractional milliliters can be collected. Be sure not to enter a volume larger than the collection tubes can contain.
Select option 4 , Prime Time. Enter a value for the priming time and press ENTER .
The Manual Sampling screen displays.
Note: The priming time is the interval the peristaltic pump runs forward, drawing medium into the system, before the valves open to deliver samples.
Select option 4 , Prime Vol. Enter a value for the priming volume and press ENTER .
The Manual Sampling screen displays.
Note: The priming volume is the amount of drawn medium necessary to fill the sampling lines of the entire system.
Select option 5 , Purge Time. Enter a value for the purging time and press ENTER .
The Manual Sampling screen displays.
Note: The purging time is the interval the peristaltic pump runs in reverse to return the uncollected medium in each line back into the vessel from which it was drawn.
Purging also clears the sampling filters of particulate matter that may restrict successive samples.
Select option 5 , Purge Vol. Enter a value for the purging volume that ensures at least two strokes of the syringe plunger and press ENTER . The Manual Sampling screen displays.
Note: The first stroke moves all medium from the sampling lines to the return lines.
The second stroke draws air into the sampling lines and purges the remaining medium from the return lines. This ensures all stranded medium is properly expelled.
Select option 6 , R-M Option. The Replacement Media Option screen displays. Select option 2 to disable media replacement. The Manual Sampling screen displays.
Note: The media replacement option is not available with the Apparatus 3 / 7. Ensure this option is disabled or the instrument will not operate properly.
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Volumetric Calibration (Peristaltic Pump only)
NOTE
The CAL key on the 8000 can be used only if the peristaltic pump is installed on your system. To calibrate the syringes in the 806 Syringe
Pump, see “5 Full Stroke (hidden option)” under “Main Menu Option 3
- Set Syringe Pump” on page 80“.
NOTE
It is critical that sample volumes are accurately and precisely taken.
Calibration of your system ensures this by recording in memory the time required to deliver a nominal 10 mL of medium into the graduated calibration tubes. Refer to
Calibration” on page 99 for specific operating procedures necessary to
calibrate your 8000.
Ensure the cannula filters are in place during calibration.
Automatic Calibration
To perform automatic calibration, complete the following steps:
1 Ensure the sample cannulas of the dissolution apparatus are sufficiently submerged in water.
2 Ensure the correct prime and purge times have been entered via the
Main Menu options 4 and 5, Manual Prime Time and Manual Purge
Time, respectively.
3 Ensure the supplied autocalibration block is firmly in place and connected to the rear panel jack labeled AUTO CALIBRATION.
4 From the Ready screen, press CAL . The dispensing arm moves forward to allow you to check the autocalibration block.
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NOTE
5 The 8000 system monitor displays a reminder to ensure the tubing and pump are ready. The Calibrate Sampling Volume screen displays.
***CALIBRATE SAMPLING VOLUME***
CHECK PUMP & TUBING. IF AUTO CALIBRATION
INSTALL CALIB FIXTURE BEFORE STARTING!
1 MANUAL 2 AUTOMATIC
6 Select option 2 , Automatic. The dispensing arm moves over the autocalibration block and primes the lines according to the manual prime settings. At the end of the priming cycle, all valves lower, open simultaneously, and begin to fill the autocalibration block. As the 10 mL point is reached in each well, the valve closes and the time value is recorded in memory. When the last valve closes, the pump reverses and purges the lines. The calibration completion alarm, if enabled, sounds when the calibration sequence is finished. Press any key to silence the alarm.
Because of intrinsic variations in tubing diameters, internal valve passages, and pump tubing tensions, some valves must stay open longer than others to deliver their nominal 10 mL calibration volumes. This is normal operation.
7 After the dispensing arm moves forward, remove the autocalibration block.
8 Clean and dry the separate portions of the autocalibration block.
9 Reconnect the top and bottom portions of the autocalibration block and replace it.
10 Press ESC to return the dispensing arm to the home position.
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Manual Calibration
CAUTION
Do not use any acidic media to calibrate your 8000. Be sure to rinse and dry the autocalibration block tips after completion.
NOTE
We suggest calibrating with tap water. Deionized water does not work well with the autocalibration block which utilizes conductivity testing.
To perform the calibration yourself, complete the following steps:
1 Remove the sample tray.
2 Ensure the sample cannulas of the dissolution apparatus are sufficiently submerged in water.
3 Ensure the correct prime and purge times have been entered via the
Main Menu option 4 and 5, Manual Prime Time and Manual Purge
Time, respectively.
4 Press MENU .
5 Select option 7 , Manual Operation.
6 Select option 4 , Goto Rows.
7 Enter 4 to move the dispensing arm to row 4.
8 Remove the media rinse reservoir.
9 Press H to return to the home position.
10 Press ESC three times to return to the Ready screen.
11 From Ready screen, press CAL . The dispensing arm moves forward to allow you to check the autocalibration block.
12 Remove the top portion of the autocalibration block and place it on its side to prevent damage.
13 Place clean, empty 10 mL calibration tubes in the bottom portion of the autocalibration block.
14 Select option 1 , Manual. The dispensing arm moves over the autocalibration block and primes the lines according to the manual prime settings.
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NOTE
15 Upon completion of the priming cycle, the following screen displays:
PRESS <OPEN VALVES> TO START
PRESS AGAIN WHEN 10 ML IS REACHED
MANUAL CALIBRATION OF VALVE # 1
TIME: 01/01/12 21:56:40 ELAPSE: 000:00:00
16 Press OPEN VALVES . The first calibration tube begins filling.
17 Observe the first calibration tube and press OPEN VALVES again when 10 mL is reached.
18 Repeat Steps 17 and 18 for the remaining calibration tubes. When the last valve is calibrated, the pump reverses and the lines are purged.
The calibration completion alarm, if enabled, sounds when the calibration sequence is finished.
19 Press any key to silence the alarm.
20 After the dispensing arm moves forward, remove and clean the calibration tubes.
21 Reconnect the top and bottom portions of the autocalibration block.
22 Press ESC .
If you press ESC before all the valves are calibrated, you cancel the calibration and return to the previously saved values for each valve.
None of the new calibration results are saved. The calibration must be allowed to finish before the new calibration values are saved.
23 Press MENU .
24 Select option 7 , Manual Operation.
25 Select option 4 , Goto Rows.
26 Enter 4 to move the dispensing arm to row 4.
27 Replace the media rinse reservoir in front of the autocalibration block.
28 Press H to return to the home position.
29 Press ESC three times to return to the Ready screen.
100 8000 Operator’s Manual
Operating the 8000 4
Checking the Calibration - Volume Accuracy
Check the calibration by running a short sampling procedure and dispensing the requested volume into calibrated test tubes:
1 Place an appropriate number of 10 mL calibration tubes in row 9 of the 8000 sample tray.
2 Ensure the sample cannulas of the dissolution apparatus are sufficiently submerged in water.
3 From the Ready screen, press MANUAL SAMPLE .
4 Enter the following parameters:
Table 26 Volume Accuracy Parameter Options
Selection
2 Set Row #
3 Set Volume
4 Prime Time
5 Purge Time
6 RM Option
Response
Enter 9 and press ENTER .
Enter 10 and press ENTER .
Enter 60 and press ENTER .
Enter 60 and press ENTER .
Ensure the RM option is disabled.
5 Verify the values for each parameter which are shown at the bottom of the display screen for this manual program.
6 If the values are correct, select option 1 , Start, to start the sampling procedure. The 8000 dispenses 10 mL into the calibration tubes in row 9 of the sample tray.
7 Check the volume in each tube to ensure the 8000 is dispensing
10 mL ± 0.5 mL from each position.
8 If the volumes are not correct, repeat the calibration of the 8000 according to the procedure described under
Calibration” on page 97 as appropriate.
If the volumes are correct, you are ready to use the 8000 to collect samples.
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4 Operating the 8000
Daisy Chaining
NOTE
A series of dissolution apparatus and 8000s can be started at the same time using one 8000 as the master unit. To set up a daisy chain, complete the following steps:
1 Connect the master 8000 START OUTPUT jack to the START
INPUT jack of its dedicated dissolution apparatus using a four-pin cable. Connect the START OUTPUT jack of the first dissolution apparatus to the START INPUT jack of a second 8000. Connect the
START OUTPUT jack of the second 8000 to the START INPUT jack of the second dissolution apparatus. Daisy chain successive units using the same technique.
2 Set the desired program for each 8000 independently.
3 For each of the remote units:
Ensure the communication port identification number for each linked
8000 increases incrementally (see “4 Set Com Port ID” under “Printer
Operation and Communications” on page 103.
• Press START PROG .
• Enter the desired program number and press ENTER .
• Select option 1 , Run Single Prog.
• Select option 3 , Remote Start.
• Select option 1 , Load & Run DISSO or APP 3/7. The program loads and the Ready screen displays.
4 For the master 8000:
• Press START PROG .
• Enter the desired program number and press ENTER .
• Select option 1 , Run Single Prog.
• Select option 1 , Start Now.
• Select option 1 , Load & Run DISSO or APP 3/7. The program starts.
102 8000 Operator’s Manual
Operating the 8000 4
NOTE
Each unit starts running the program you entered.
Ensure no start output delays have been set on any of the 8000s.
Depending on your configuration, see “3 Start Output Delay (peristaltic pump)” under
“8000 Menu Options - Apparatus 1 / 2” on page 48 or “6
Start Output Delay” under “Main Menu Option 1 - Set
Clock/Alarms” on page 51.
Printer Operation and Communications
NOTE
Ensure the printer type is set to impact for the instrument to operate properly
(MENU > 0 > E under
“Hidden Key Functions” on page 41)
Use print options to
• print batch information and sample times for each of the 15 stored programs.
• control the built-in Report Center Printer and the remote printer (if one is connected).
• set the communication port identification number and the baud rate.
Press PRINT . Enter a program number and press ENTER . The Print
Selections screen displays.
5 SET BAUD RATE
***PRINT SELECTIONS***
1 BATCH INFORMATION
3 TURN ON (OFF) REP CENTER
2 SAMPLE TIMES
4 SET COM PORT ID
8000 Operator’s Manual 103
4 Operating the 8000
Following is a description of the Print Selections screen options:
Table 27 Print Selections Options
Option
1 Batch Information
Function
Select option 1 , Batch Information, to immediately print the previously entered batch information, if any, for the selected program. This printed data is the header information you can enter via option 4, Headers, on the Program Variables screen.
2 Sample Times
3 Turn On (Off) Rep Center Select option 3 , Turn On (Off) Rep Center, to enable or disable the Report Center
Printer. This option always reflects the opposite of the current state of the Report
Center Printer. For example, if it is currently disabled, TURN ON REP CENTER displays next to option 3.
Note: The state of the Report Center Printer is not unique to an individual program.
It is global. When you enable or disable it, the Report Center Printer stays enabled or disabled until you change it again, even if you switch programs.
4 Set Com Port ID
Select option 2 , Sample Times, to immediately print the sampling times for each row of the selected program. This is the complete listing of all time points entered in option 2, Sample Time Points, under the Program Variables screen.
Select option 4 , Set Com Port ID, to enter a new communication port identification number. Your system was shipped with the communication port identification number set to 01. You can set the identification number to any number between 1 and 99. The identification number is used when multiple units are connected in series so that the controlling or master 8000 can start individual units.
Enter the communication port identification number and press ENTER . The Print
Selections screen displays.
5 Set Baud Rate Select option 5 , Set Baud Rate, to set the baud rate for your system. If you are using the 8000 with other Agilent equipment, set the baud rate to 9600 baud (option 4).
Select the desired baud rate from the menu. The Print Selections screen displays.
Note: In order to set the baud rate from this option, the baud rate must be set to
programmable baud (see MENU > 0 > 8 under “Hidden Key Functions” on page 41.
104 8000 Operator’s Manual
Agilent 8000 Dissolution Sampling Station
Operator’s Manual
5
Maintenance and Troubleshooting
Report Center Impact Printer 119
Agilent Technologies
105
5 Maintenance and Troubleshooting
Preventive Maintenance
WARNING
The 8000 and accessories contain electrical circuits, devices, and components operating at dangerous voltages. Contact with these circuits, devices, and components can cause death, serious injury, or painful electric shock.
Perform the following maintenance when necessary:
Clean System with Peristaltic Pump
Use the Clean System function often to keep the valves, needles, and sample lines clean. The timing of the valves opening and closing with the pump rotations creates cavitation in the valves. Cleaning the system dislodges any particulate matter that may affect the operation of the system. To prolong proper functionality of the 8000 system, it is recommended to perform a cleaning cycle after each dissolution test program.
NOTE
Prior to beginning the clean system function, verify an appropriate manual prime time and purge time have been entered via the Main
Menu.
Apparatus 1 and 2
1 Place the rinse tray on the vessel table of the dissolution apparatus.
2 Fill the rinse tray with an appropriate cleaning solution, usually water.
3 Fill the rinse chamber of the media rinse reservoir with an appropriate cleaning solution, usually water.
4 Lower the sampling cannulas. See the appropriate dissolution apparatus operator’s manual for information on how to operate the sampling cannulas.
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Maintenance and Troubleshooting 5
5 On the 8000, press CLEAN SYSTEM . The Cleaning Systems
Selections screen displays.
***CLEANING SYSTEMS SELECTIONS***
1 START CLEAN 2 ENABLE AUTO CLEAN
TIME: 01/01/12 08:28:46 ELAPSE: 000:00:00
Apparatus 3 / 7
1 Place the appropriate number of outer media tubes in Row 1 of the
Apparatus 3 / 7.
2 Fill each outer media tube with an appropriate cleaning solution, usually water.
3 From the Main Menu, select option 6 , Control APP 3/7. See
4 Select option 3 , Goto Row.
5 Enter 1 and press ENTER to move the drive unit to Row 1 which contains the outer media tubes filled with cleaning solution.
6 Select option 1 , Dip, to lower the agitator shafts and begin dipping. A
slow dipping speed, such as 5 DPM, is recommended. See “1
DIPPING SPEED (DPM)” on page 79 for information about how to
set the dipping speed.
7 Once dipping has started, press CLEAN SYSTEM on the 8000. The
Cleaning Systems Selections screen displays.
***CLEANING SYSTEMS SELECTIONS***
1 START CLEAN 2 ENABLE AUTO CLEAN
TIME: 01/01/12 08:28:46 ELAPSE: 000:00:00
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5 Maintenance and Troubleshooting
Start Immediately
From the Cleaning Systems Selections screen, select option 1 , Start
Clean. The 8000 immediately starts to clean the system by priming the lines. Once the system completes the priming, the needle manifold jogs up and down while opening and closing the valves. This motion rinses the needles in the 8000 rinse chamber. Then the system purges the cleaning solution from the sample lines.
The following figure is a generalized flow diagram of the clean cycle:
Start
Place Probes in Rinse
Solution
Prime Tubing
Move
Head to Rinse
Position
Open/Close
Valves
Reverse Pump
Open/Close
Valves
Raise/Lower
Needles
Purge Tubing
(Clear Filters)
Done
Figure 16 The Cleaning Process
Programmed Clean
To run a program concurrently with the sampling program which rinses the needles in a predetermined cleaning solution contained in the rinse chamber, complete the following steps:
1 Ensure the rinse chamber of the media rinse reservoir is filled with the cleaning solution.
2 From the Cleaning Systems Selections screen, select option 2 ,
Enable Auto Clean. Option 3, Disable Auto Clean, displays on the
Cleaning Systems Selections screen and option 2 changes to Program
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Maintenance and Troubleshooting 5
NOTE
NOTE
Auto Clean.
***CLEANING SYSTEMS SELECTIONS***
1 START CLEAN 2 PROGRAM AUTO CLEAN
3 DISABLE AUTO CLEAN
TIME: 01/01/12 ELAPSE: 000:00:00
Select option 3, Disable Auto Clean, to escape the automatic clean function and return to the original Cleaning Systems Selections screen.
Select option 1, Start Clean, from either screen to escape the automatic clean function and immediately start the cleaning process.
3 Select option 2 , Program Auto Clean. The Program Auto Clean
Variables screen displays.
***PROGRAM AUTO CLEAN VARIABLES***
1 CLEAN FREQUENCY
3 PUSH FORWARD TIME
2 DRAWING MEDIA TIME
TIME: 01/01/12 08:28:46 ELAPSE: 000:00:00
Since the cleaning program runs concurrently with the sampling program, the value entered for the clean frequency should be smaller than the sample time point.
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5 Maintenance and Troubleshooting
4 Select each option and enter the appropriate parameters as necessary based on tubing length, tubing diameter, and system dead volumes.
Table 28 Program Auto Clean Variables
Option
1 Clean Frequenc y
2 Drawing Media Time
3 Push Forward Time
Function
Select option 1 , Clean Frequency. Enter an interval of up to 99 minutes and press
ENTER . The cleaning begins at this time.
Note: Ensure the clean frequency is set earlier than the first sample time point.
Select option 2 , Drawing Media Time. Enter an interval of up to 999 seconds to control the length of time the cleaning solution is drawn from the rinse chamber and press ENTER .
Select option 3 , Push Forward Time. Enter an interval of up to 999 seconds to indicate the length of time the solution is moved through the return lines and press
ENTER .
NOTE
NOTE
Appropriate parameters for each of the above options depends on the length and diameter of the tubing as well as the needle volume. Do not allow the cleaning solution to reach the dissolution apparatus. Once the pump reverses, the cleaning solution is expelled in the rinse chamber.
At the time set for the clean frequency (see “1 Clean Frequency” above):
• the 8000 drive unit moves to the rinse chamber and lowers the needles
• the peristaltic pump draws the cleaning solution into the needles and sample lines for the length of time defined as the drawing media time
(see “2 Drawing Media Time” above)
• the valves close and the pump pushes the cleaning solution into the return lines for the programmed push forward time (see “3 Push
Forward Time” above).
Ensure the length of time entered for the push forward time does not push the cleaning solution far enough to reach the dissolution apparatus or sample contamination can occur.
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Maintenance and Troubleshooting 5
• the peristaltic pump reverses and the cleaning solution is pulled back through the sample lines
• the pump reverses again, the valves open and the cleaning solution is expelled into the rinse chamber
• the 8000 activates the valves several times to clean the valves and needles.
Clean System Function with Syringe Pump
Apparatus 1 / 2
1 Place the rinse tray fitted with cleaning solution, usually water, on the vessel table of the dissolution apparatus.
2 Immerse all six or eight sampling cannulas.
3 Press CLEAN SYSTEM .
4 The 8000 immediately starts to clean the system.
8000 Operator’s Manual
Apparatus 3 / 7
To clean the system, immerse all sampling cannulas into a cleaning solution, usually water. Complete the following steps:
1 Fill one row of outer media tubes with the appropriate cleaning solution, usually water.
2 On the Apparatus 3 / 7, press START DIP .
3 Select option 3 , Goto Row.
4 Enter the row number which contains the cleaning solution and press
ENTER . The drive unit moves to the selected row.
5 Select option 2 , Set Speed (DPM).
6 Enter 5 DPM and press ENTER .
7 Select option 1 , Start. The agitator shafts lower and begin dipping.
8 On the 8000, press CLEAN SYSTEM . The Cleaning Systems
Selections screen displays and the cleaning procedure begins immediately.
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5 Maintenance and Troubleshooting
9 Upon completion of the cleaning process, the 8000 dispensing arm and the Apparatus 3 / 7 drive unit return to the home position.
Syringe and Seal Replacement
NOTE
If the syringe seal eventually develops a leak, retighten the syringe by hand (18 - 21 oz-in, 1.3 - 1.5 Kg-cm).
If the seal eventually obstructs the media path, new seals are available for purchase (P/N K1002-00438).
CAUTION
1 Disconnect all sample tubes from the pump.
2 On the 8000, press MENU > 7 > 6 > 3 to partially extend the syringe plungers.
3 When the syringe plungers reach approximately 5 mL, turn off the pump.
4 Disconnect the power cord and communication cables from the pump.
5 Remove the syringe plunger thumb screw(s).
6 Using a #2 Phillips screwdriver, remove the 4 screws that secure the retainer block at the top of the syringes.
7 Carefully remove the syringe valve retainer block.
8 Flip the pump so that it is sitting on its top.
9 For improved access to the syringe ferrule, gently slide the syringe away from the pump while disengaging the plunger and valve from their locating pins.
The distance that the valve will move out of the pump is limited by the valve wires. Use care not to break or damage the valve wires.
10 Unscrew the syringe from the valve using care not to break the glass.
11 If there is a seal within the syringe port on the valve, extract it from the port.
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Maintenance and Troubleshooting 5
NOTE
12 Install the new seal into the syringe port, making sure that it lays flat in the bottom of the port and that the media path is not obstructed.
13 Install the syringe into the valve. Tighten by hand (18 - 21 oz-in,
1.3 - 1.5 Kg-cm).
14 Extend syringe plunger so that it is aligned with the plunger pin on the pump.
15 Carefully install the syringe and valve assembly onto the valve and plunger pins.
16 Replace the syringe valve retainer block and tighten the screws.
17 Reinstall the syringe plunger thumb screw(s).
18 Place the pump upright and connect the power cord and communication cables.
19 Turn on the pump.
The pump will initialize and compress all of the syringe plungers when turned on.
20 Connect all sample tubes to the pump.
21 Check for leaks using the 8000.
Cleaning the Syringes
To clean the syringes, use the appropriate cleaning solution based on the product being tested. Ensure the sampling cannulas of the dissolution apparatus are submerged into the cleaning solution. Syringes should be cleaned after each test using one of the following procedures:
50% Methanol or 10% Bleach Cleaning Procedure
1 From the 8000 Ready screen, press MENU > 7 . The Manual
Operations screen displays.
2 Select option 6 , Syringe/Filter. The Operate Syringe/Filter Changer screen displays.
3 Select option 1 , Valve to Input, to ensure the valve is set to input.
4 Select option 3 , Syringe Fill.
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5 Maintenance and Troubleshooting
5 Enter 100 and press ENTER . The syringes fill completely with cleaning solution.
6 Allow the solution to remain in the syringes for ten minutes.
7 Select option 4 , Syringe Dispense. The cleaning solution expels from the syringes.
8 Replace the cleaning solution in the rinse tray with ultrapure water.
9 Repeat Steps 3 - 7 a minimum of ten times to flush the syringes thoroughly.
10 When the cleaning procedure is complete, press ESC until the Ready screen displays.
Acid / Base Cleaning Procedure
1 Fill the rinse tray with 0.1N NaOH.
2 From the 8000 Ready screen, press MENU > 7 . The Manual
Operations screen displays.
3 Select option 6 , Syringe/Filter. The Operate Syringe/Filter Changer screen displays.
4 Select option 1 , Valve to Input, to ensure the valve is set to input.
5 Select option 3 , Syringe Fill.
6 Enter 100 and press ENTER . The syringes fill completely with cleaning solution.
7 Allow the solution to remain in the syringes for ten minutes.
8 Select option 4 , Syringe Dispense. The cleaning solution expels from the syringes.
9 Replace the cleaning solution in the rinse tray with ultrapure water.
10 Repeat Steps 5 - 9 to flush the syringe.
11 Change the cleaning solution in the rinse tray to 0.1N HCl and repeat
Steps 5 - 9.
12 Replace the cleaning solution in the rinse tray with ultrapure water.
13 Repeat Steps 5 - 9 a minimum of ten times to flush the syringes thoroughly.
14 When the cleaning procedure is complete, press ESC until the Ready screen.
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Maintenance and Troubleshooting 5
System Cleaning Procedure with Syringe Pump and
Filter Changer (optional)
To clean the 8000, syringe pump, and filter changer system, use the appropriate cleaning solution based on the product being tested. Ensure the sampling cannulas are submerged into the cleaning solution.
Complete the following steps to set up the cleaning procedure:
NOTE
Ensure the RM option is disabled by pressing MENU > 0 > R .
1 From the Ready screen, press PROG to program the 8000.
2 Enter a program number (1 - 15) to use for this cleaning program and press ENTER . The Program Variables screen displays.
3 Select option 1 , Set DISSO Parameters.
4 Enter the following parameters:
Table 29 Disso Parameters.
Selection
1 Set Spindle RPM
2 Final Spin Length
3 Ini/Final Vessel Temp
4 Set Bath Temp
5 Com Port ID
6 DDM or Baskets
7 Print Freq
Response
Enter 50 and press ENTER .
Enter 0 and press ENTER .
Select option 2 , Disable, for each selection.
Enter 37.3
and press ENTER .
Note: It is not necessary to set the water bath temperature if using a dissolution apparatus with Direct Vessel Heating (DVH).
Enter 01 and press ENTER .
Select option 2 , Paddles. Select option 2 , Simultaneous.
Enter 000 and press ENTER .
5 Press ESC > Y . The Program Variables screen displays.
8000 Operator’s Manual 115
5 Maintenance and Troubleshooting
6 Enter the following parameters:
Table 30 Program Variables
Selection
2 Sample Time Points
3 Sample Volume
4 Set Headers
5 Prime Volume
6 Purge Volume
7 Filter Chg
Response
Row 0: Enter 000:05 and press ENTER .
Row 1: Enter 000:10 and press ENTER .
Row 2: Enter 000:15 and press ENTER .
Row 3: Enter 000:00 and press ENTER .
Enter a sample volume based on the size vial used in the 8000 sample tray and press ENTER . Ensure Rows 0 - 2 in the sample tray contain empty vials.
Enter the requested information as applicable. Press ESC to return to the
Program Variables screen.
Enter 10 and press ENTER .
Enter 15 and press ENTER .
This option is only applicable if your system configuration includes a filter changer.
Set the number of samples per filter to 1 and press ENTER .
7 Press ESC until the Ready screen displays.
8 Press START PROG .
9 Enter the program number for this cleaning program and press
ENTER .
10 Press 1 > 1 > 1 > 1 to start the cleaning program.
After the 8000 pulls three rows of samples for cleaning purposes, complete the following steps to ensure the needles are completely purged of cleaning solution:
1 From the Ready screen, press MENU > 7 > 4 > R .
2 Press ESC . The Manual Operations screen displays.
3 Select option 3 , Open/Close Valves, to open the valves.
4 Select option 2 , Lower Valves, to lower the needles.
5 Select option 1 , Lift Valves, to raise the needles.
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Maintenance and Troubleshooting 5
6 Repeat Steps 4 and 5 a minimum of four times to completely expel the cleaning solution from the needles.
7 Select option 3 , Open/Close Valves, to close the valves.
8 Select option 4 , Goto Rows.
9 Press H to move the dispensing arm to the home position.
10 Press ESC until the Ready screen displays.
Cleaning the Filter Changer Drip Tray
1 Slide up and remove the filter catch basin from the screw mount on the front of the filter changer.
2 Pull the drip tray forward completely to remove it from under the filter changer.
3 Empty any accumulated liquid from the tray and wipe it clean.
4 Replace the tray under the filter changer by sliding it forward along the tracks until it stops.
5 Replace the filter catch basin.
Cleaning the Media Replacement Pump
It is necessary to clean the media replacement pump. The following instructions are a general cleaning procedure. Additional cleaning may be required depending on the media used.
1 From the Ready screen, press MENU > 7 .
2 Select option 4 , Goto Rows. The Select Destinations screen displays.
3 Press 6 to move the dispensing arm to Row 6.
4 Remove the rinse reservoir, empty any fluid and replace the reservoir.
5 Press H to return the dispensing arm to the home position.
6 Fill a 1L beaker or vessel with warm water.
7 Place the source tube in the warm water.
8 Place the return line in an empty beaker or vessel.
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5 Maintenance and Troubleshooting
9 Press ESC to return to the Manual Operations screen.
10 Select option 5 , Turn On R-M Pump.
11 Allow the pump to run until the source beaker is empty.
12 Select option 5 , Turn Off R-M Pump.
13 Repeat Steps 2 - 12 using ultrapure water in place of the warm water.
14 Select option 5 , Turn On R-M Pump.
15 Purge the system by removing the source line from the ultrapure water and allowing the pump to run until water no longer flows through the return line.
16 Select option 5 , Turn Off R-M Pump.
17 From the Manual Operations screen, select option 4 , Goto Rows.
18 Press 6 to move the dispensing arm to Row 6.
19 Remove the rinse reservoir, empty any fluid, and replace the reservoir.
20 Press H to return the dispensing arm to the home position.
21 Press ESC until the Ready screen displays.
If additional cleaning is required, repeat the above procedure replacing the warm water with diluted alcohol (1:10).
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Maintenance and Troubleshooting 5
Report Center Impact Printer
The following is helpful information for using your impact printer.
Installing the Cartridge Ribbon
If the printer is used infrequently, the print impression sometimes becomes weak because the ribbon dries out. If the printed material is difficult to read and you suspect this is the cause of the problem, advance to a properly inked portion of the ribbon by pressing the printer toggle switch into the Paper feed position. If the printing is still faint, replace the cartridge.
To install the cartridge, complete the following steps:
1 Toggle the printer off line by pressing the printer toggle switch to the
OnLine / Off Line position. When the printer is off line, the Ready
LED does not illuminate.
2 Four small grooves are embossed on the printer cover. Gently push on these grooves to tilt the cover. When the printer cover is tilted up, you can lift it off completely.
3 Push down on the right side of the ribbon cartridge (marked PUSH) and remove the old cartridge.
4 Install the new cartridge. If there is already paper in the printer, hold the cartridge between your thumb and index finger, slide it over the paper and into the printer compartment. Ensure the paper is between the ribbon cartridge and the ink ribbon. Ensure the ink cartridge is inserted firmly to prevent weak or irregular printing. The cartridge must be properly seated and aligned for the best printing.
5 Turn the cartridge knob (marked by an arrow) clockwise to stretch the ribbon taut.
6 Replace the cover.
7 Toggle the printer online by pressing the printer toggle switch to the
OnLine / Off Line position. The Ready LED illuminates.
8 Replace the paper, if necessary.
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5 Maintenance and Troubleshooting
If you get ribbon ink on the printer’s plastic cover, remove it immediately. Once dried, it is difficult to remove.
Replacing a Paper Roll
CAUTION
1 Toggle the printer off line by pressing the printer toggle switch to the
OnLine / Off Line position. When the printer is off line, the Ready
LED does not illuminate.
2 Grasp the paper roll cover firmly by the grooves on the side and the front edge. Pull outward to remove the cover.
3 Press the printer toggle switch to Paper feed to advance the paper approximately one inch beyond the paper cutter.
4 Remove the paper roll.
5 Using scissors, cut the paper feeding to the printer.
6 Pull the remaining paper through the printer mechanism. Pull the paper from the front (paper cutter side) . Pulling the paper out of the back of the printer will damage the print mechanism.
7 Unroll several inches of paper on the new roll.
8 If it is jagged, cut a straight edge on the paper roll to facilitate the entry of the paper into the printer.
9 Slide the paper through the slot connecting the paper compartment and the printer compartment. You can slide it in approximately 1/4 inch before it stops.
10 While holding the paper in place, press the printer toggle switch to the Paper feed position and hold until approximately one inch of
.
paper has emerged from the top of the printer
Ensure the roll of paper feeds squarely. If it does not, the paper can jam and possibly damage the printer mechanism.
11 Release the printer toggle switch.
12 Turn the paper roll to take up any slack in the paper feeding to the printer. Place the paper roll in the paper compartment.
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Maintenance and Troubleshooting 5
13 Replace the paper roll cover. If the cover is difficult to remove or replace, the left and right edges can be trimmed or shaved with a utility knife allowing the cover to slide easier.
14 Toggle the printer online by pressing the printer toggle switch to the
OnLine / Off Line position. The Ready LED illuminates.
Toggling Your Printer Online
Complete these steps to toggle your printer online:
1 Toggle the printer online by pressing the printer toggle switch to the
OnLine / Off Line position. When the printer is off line, the Ready
LED does not illuminate.
2 Release the switch and it returns to the center position. The ready
LED illuminates and a READY message prints if the PRINT READY
PRINT READY command. When you first turn on the instrument, it prints a READY message to assure you that the built-in microprocessor is operating properly.
When you turn off the printer, wait at least three seconds before turning it back on.
Printer Self Test
You can test the print head and ribbon only after inserting paper. Do not attempt to print without paper. Follow these steps to perform a printer self test:
1 Turn off the 8000.
2 Press and hold the printer toggle switch in the Paper feed position.
3 Turn on the 8000.
4 Hold the printer toggle switch until printing begins. The printer prints a list of the current configuration settings and a continuous print test.
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5 Maintenance and Troubleshooting
5 Press the printer toggle switch to the OnLine / Off Line position to stop the printing operation.
6 The printer is ready to resume normal operation.
Printer Configuration
NOTE
The printer configuration is set by the factory. This procedure should be performed only if the printer displays erroneous characters. Contact the
Dissolution Systems Service Department for assistance, if necessary.
NOTE
1 Turn off the 8000.
2 Press and hold the printer toggle switch in the OnLine / Off Line position while turning the 8000 back on. Hold the printer toggle switch in the OnLine / Off Line position for 6 seconds after the instrument is turned on, then release the switch.
3 The printer should print: *** SETUP MENU *** and CONFIGURE ... .
[ NEXT / OK ]. If this message does not print, repeat Steps 1 through 3.
4 The printer toggle switch is used to complete the configuration.
Pressing the left side of the toggle switch selects NEXT to advance to the next menu item. Pressing the right side of the toggle switch selects OK to accept what is stated on the current line of the menu item. Each time the switch is pressed, another part of the menu prints. Allow the printer to finish printing before pressing the switch again. See the table of commands on the following page.
The printout is easier to read if the print cover is removed.
122 8000 Operator’s Manual
Maintenance and Troubleshooting 5
*** SETUP MENU***
CONFIGURE [NEXT/OK]
CUSTOM [NEXT/OK]
***CUSTOM MENU***
PRINT CUSTOM SETUP [NEXT/OK]
AUTO SEQ = NO [NEXT/OK]
ZERO = Ø [NEXT/OK]
POUND SIGN = # [NEXT/OK]
_(UNDERSCORE) [NEXT/OK]
ONLINE/OFFLINE=YES [NEXT/OK]
EXT CH SET = NO [NEXT/OK]
PRINT READY = YES [NEXT/OK]
PRINT READY = NO [NEXT/OK]
READY...
Press NEXT to avoid configuration.
Press OK to enter custom mode.
Press NEXT .
Press OK .
Press OK .
Press OK .
Press OK .
Press OK .
Press OK .
Press NEXT .
Press OK .
Obtaining Warranty and Other Services
To place a service order (warranty or other services), please contact your local Customer Care Center. Contact information can be found at www.agilent.com under your country using the Contact Us link. Place your service request using the displayed phone number or E-mail address.
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124 8000 Operator’s Manual
Index
Numerics
8000 clamp removal,
unpacking,
A aspirt. dwell time,
,
Autocalibration Block,
automatic calibration,
B
Batch Information,
C calib compl alarm,
calibration, checking,
clean frequency,
clean system,
com port id,
connections, power and network,
control APP 3/7,
control DISSO,
Conventions,
D ddm or basket,
Dipping Speed,
drawing media time,
drop volume,
dual voltage option,
F filter changer change filters,
filters,
operate,
power connections,
final spin length,
full flow filters,
G goto rows,
H hidden key functions,
I ini / final vessel temp,
instant start, DISSO,
K keypad,
manual sample,
options,
start prog,
L lift valves,
linking programs,
load & run APP 3/7,
lower valves,
M main menu, 8000,
8000 Operator’s Manual
Main Screen
Options,
manual calibration,
manual operation,
manual sample,
,
maximum volume,
modify program,
O open valves,
open/close valves,
,
Operating,
P peristaltic pump main menu,
,
power connections,
unpacking,
plunger speed,
,
Preventive Maintanance,
prg compl alarm,
prime loss volume,
prime time,
prime volume,
,
printer cartridge ribbon,
operation,
removing paper roll,
self test,
toggling online,
program variables screen,
Purge Time,
purge time,
Purge Volume,
purge volume,
,
push forward time,
125
Index
R ready screen,
removing paper roll,
replacement media (rm) installing external,
installing internal,
replacement media (rm) option,
report center printer operation,
row compl alarm,
rs232,
run 8000 only,
run wo/ loading,
S sample time points,
sample timepoints,
Sample Times,
sample tray, installing,
sample tubing connections,
,
screens initial status,
ready,
system monitor,
set alarms,
set APP 3/7,
set bath temp,
Set Baud Rate,
set clock,
set clock / alarms,
Set Com Port ID,
set dipping speed,
set DISSO,
set row number,
set spindle rpm,
set syringe pump,
set vessel temp and start,
set volume,
setting alarms,
start clean,
start mode,
start program,
syringe / filter,
syringe dispense,
syringe pump enabling,
fill syringe,
main menu,
operate,
power connections,
set syringe size,
setting,
,
syringe dispense,
syringe parameters,
unpacking,
syringe to fill,
T timer alarm,
turn on / off r-m pump,
V valve to input,
,
valve to output,
Volumetric Calibration,
126 8000 Operator’s Manual
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