Lumenera Scientific Infinity Camera User`s Manual

Lumenera Scientific
Infinity Camera
User’s Manual
Lumenera Scientific
2470 Don Reid Drive, Ottawa, Ontario, Canada. K1H 1E1
Tel: (613) 736-4077 • Fax: (613) 736-4071
www.lumenera-scientific.com
Lumenera Scientific Infinity Camera
User’s Manual
Release 3.3
Copyright Notice:
Release 3.3
Copyright © 2004 Lumenera Corporation. All rights reserved.
The contents of this document may not be copied nor duplicated in any form, in whole or in part,
without prior written consent from Lumenera Corporation. Lumenera makes no warranties as to
the accuracy of the information contained in this document or its suitability for any purpose. The
information in this document is subject to change without notice.
License Agreement (Software):
This Agreement states the terms and conditions upon which Lumenera Corporation ("Lumenera")
offers to license to you (the "Licensee") the software together with all related documentation and
accompanying items including, but not limited to, the executable programs, drivers, libraries, and
data files associated with such programs (collectively, the "Software").
The Software is licensed, not sold, to you for use only under the terms of this Agreement.
Lumenera grants to you the right to use all or a portion of this Software provided that the Software
is used only in conjunction with Lumenera's family of products.
In using the Software you agree not to:
a) decompile, disassemble, reverse engineer, or otherwise attempt to derive the source code for
any Product (except to the extent applicable laws specifically prohibit such restriction);
b) remove or obscure any trademark or copyright notices.
Limited Warranty (Hardware and Software):
ANY USE OF THE SOFTWARE OR HARDWARE IS AT YOUR OWN RISK. THE SOFTWARE IS
PROVIDED FOR USE ONLY WITH LUMENERA'S HARDWARE AND OTHER RELATED
SOFTWARE. THE SOFTWARE IS PROVIDED FOR USE "AS IS" WITHOUT WARRANTY OF
ANY KIND. TO THE MAXIMUM EXTENT PERMITTED BY LAW, LUMENERA DISCLAIMS ALL
WARRANTIES OF ANY KIND, EITHER EXPRESS OR IMPLIED, INCLUDING, WITHOUT
LIMITATION, IMPLIED WARRANTIES OR CONDITIONS OF MERCHANTABILITY, QUALITY
AND FITNESS FOR A PARTICULAR PURPOSE. LUMENERA IS NOT OBLIGATED TO
PROVIDE ANY UPDATES OR UPGRADES TO THE SOFTWARE OR ANY RELATED
HARDWARE.
Limited Liability (Hardware and Software):
In no event shall Lumenera or its Licensor's be liable for any damages whatsoever (including,
without limitation, incidental, direct, indirect, special or consequential damages, damages for loss
of business profits, business interruption, loss of business information, or other pecuniary loss)
arising out of the use or inability to use this Software or related Hardware, including, but not
limited to, any of Lumenera's family of products.
Trademarks and Proprietary Names:
Lumenera Scientific, Infinity X, Infinity 1, Infinity 2 and DeltaVU are trademarks of Lumenera
Corporation. This manual may refer to other product names, which may be trademarked by their
respective companies, and are used herein for identification purposes only.
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Federal Communications Commission (FCC) Statement
This equipment has been tested and found to comply with the limits of a Class B digital device, pursuant
to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful
interference when the equipment is operated in a residential environment. This equipment generates,
uses, and radiates radio frequency energy, and if not installed and used in accordance with the
instructions, may cause harmful interference. However, there is no guarantee that interference will not
occur. If this equipment does cause interference to radio or television reception, which can be
determined by turning the equipment off and on, the user is encouraged to correct the interference by one
of the following measures:
•
•
•
•
Reorient or relocate the receiving antenna.
Increase separation between the equipment and receiver.
Connect the equipment into an outlet on a circuit different from which the receiver is connected.
Consult the dealer or an experienced radio/TV technician.
Any changes or modification to said product not expressly approved by Lumenera could void the user's
authority to operate this device.
Shielded and grounded cables and connectors must be used to meet FCC emission limits. Lumenera
takes no responsibility for any radio or television interference caused by using cables or connectors other
than those recommended.
This device complies with part 15 of the FCC Rules. Operation is subject to the following two conditions:
(1) This device may not cause harmful interference, and (2) this device must accept any interference
received, including interference that may cause undesired operation.
European Community (CE) Statement
Products: Infinity 1, 2, 3, 4, 5, X, Lu1175, Lu1175X, Lu1205, Lu1135, Lu1165, Lu1275, Lu1335 and
Lu1375 USB Scientific Cameras.
Directives: 2001/59/EC (General Product Safety Directive) Self-declared 89/336/EEC (EMC Directive).
Standards to which conformity is declared:
Manufacturer’s Name and Address:
EN55024: 1998
EN55022: 1998 (Class B)
EN61000-3-2: 1995
EN61000-3-3: 1995
Lumenera Corporation
2470 Don Reid Drive.
Ottawa, ON
K1H 1E1
Canada
Type of Equipment: USB 2.0 Scientific Digital Camera
First Year of CE Conformity: 2003
This is to certify that the Lumenera USB 2.0 Scientific Cameras meet or exceed the standards for CE
compliance per the Council Directives noted above. All equipment is built at Lumenera Corporation, and
pertinent testing documentation is available for verification.
Copyright  2004
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User’s Manual
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Table of Contents
INTRODUCTION ......................................................................................................................... 1
1.1
THE LUMENERA SCIENTIFIC INFINITY CAMERA FAMILY .................................................. 1
GETTING STARTED .................................................................................................................. 2
2.1
SOFTWARE INSTALLATION ............................................................................................... 2
2.1.1
System Requirements............................................................................................... 2
2.1.2
Installation Procedure............................................................................................. 3
2.2
TECHNICAL SUPPORT ....................................................................................................... 4
2.3
INSTALLED SOFTWARE ..................................................................................................... 4
2.4
THE INFINITY CAMERA..................................................................................................... 5
USING THE CAMERA................................................................................................................ 6
3.1
INFINITY CAPTURE ........................................................................................................... 6
3.2
LAUNCHING THE APPLICATION ........................................................................................ 6
3.2.1
Main Window .......................................................................................................... 6
3.3
SELECTING IMAGE SIZE .................................................................................................... 7
3.3.1
Image Preview Size ................................................................................................. 7
3.3.2
Capture Resolution.................................................................................................. 8
3.4
IMAGE OPTIMIZATION ...................................................................................................... 8
3.4.1
Exposure.................................................................................................................. 8
3.4.2
Gain......................................................................................................................... 9
3.4.3
Selecting the Light Source....................................................................................... 9
3.4.4
White Balance Target............................................................................................ 10
3.4.5
White Balance ....................................................................................................... 11
3.4.6
Hue and Saturation ............................................................................................... 13
3.4.7
Contrast / Brightness / Gamma ............................................................................. 14
3.5
CAPTURING AN IMAGE ................................................................................................... 15
3.5.1
Capture Image....................................................................................................... 15
3.6
OTHER OPTIONS ............................................................................................................. 16
3.6.1
Light Source Frequency ........................................................................................ 16
3.6.2
Preview Quality..................................................................................................... 16
3.6.3
Preview Zoom Level .............................................................................................. 17
3.6.4
Generate Flat Field Correction ............................................................................ 17
3.6.5
Enable Flat Field Correction................................................................................ 17
3.6.6
Image Flipping ...................................................................................................... 17
3.6.7
Multiple Image Averaging..................................................................................... 17
3.6.8
Preview Histogram................................................................................................ 18
3.6.9
Highlight Saturated Pixels .................................................................................... 18
3.6.10
Load Overlay Bitmap ............................................................................................ 18
3.6.11
Load Alpha Blending Bitmap ................................................................................ 18
3.6.12
Load Settings / Save Settings / Save Settings As ................................................... 19
3.7
USING INFINITY CAMERAS WITH TWAIN ...................................................................... 20
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1 1
Introduction
1.1
The Lumenera Scientific Infinity Camera Family
Lumenera Scientific Infinity Cameras are designed to be flexible microscopy
imaging tools for Clinical, Research, Life Science, Materials Science and
Educational Professionals. Infinity cameras are compatible with any USB 2.0
equipped desktop, laptop or embedded computer.
Infinity cameras are designed with flexibility in mind. Each camera model has its
own distinct advantage over the others. Whether your application demands
speed, resolution or sensitivity, there is an affordable Infinity Camera to meet the
requirements. Because they are USB based, there is no need for a frame
grabber. Instead, a single cable provides power*, full command and control and
data transfer at speeds of up to 24 MB/s resulting in real-time video preview.
All cameras have a provision to be externally powered for cases where the USB
cable does not supply power (e.g. some USB cards on laptop computers.)
* The Infinity 2, 4, 5 and X models should be used with an external power adapter (supplied) due
to their higher current requirements.
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2
Getting Started
2.1
Software Installation
The Infinity High-Speed USB 2.0 camera you have just purchased is designed to
operate out of the box with minimal set-up.
Prior to plugging the camera into the computer, you must first install the
software.
The software can be found on the CD-ROM that shipped with your product.
2.1.1 System Requirements
We recommend that your PC is equipped with one of the following Microsoft
Windows operating systems:
•
Windows 2000 (Service Pack 3), or
•
Windows XP (Service Pack 1)
•
Microprocessor:
¾ Recommended: Pentium® 4, 1.3 GHz or more
¾ Minimum: 600 Mhz
•
Memory:
¾ Recommended: 512 Mb of RAM
¾ Minimum: 256 Mbytes of RAM
•
USB 2.0 Port.
Note: A USB 2.0 Port is required. The camera will not work
on a standard USB 1.1 port. USB 2.0 cards for desktop and
laptop computers are available as accessories through your
camera distributor or most electronics stores.
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2.1.2 Installation Procedure
Please install your new Infinity camera in the following order:
Note: Do not connect your infinity camera before installing
the infinity camera software.
1. USB 2.0 add-in card.
• If you are using a 3rd party USB 2.0 PCI add-in card, please ensure the
add-in card is properly installed on your computer before proceeding
•
We recommend that you use the built-in operating systems USB 2.0
Enhanced Host Controller Interface (EHCI) drivers provided by
Microsoft rather than the device drivers supplied with your 3rd party card
•
The built-in operating systems USB 2.0 Enhanced Host Controller
Interface (EHCI) drivers provided by Microsoft are built-in when using
Windows XP with Service Pack 1.
•
Windows 2000 users should first upgrade to Service Pack 4, and then go
to the Windows upgrade site: http://windowsupdate.microsoft.com to
obtain the USB 2.0 Enhanced Host Controller Interface (EHCI) drivers
2. Infinity Camera Software
• You must be logged into the computer with administrator privileges prior to
continuing the installation.
•
Close all application software that is running and then insert the Infinity
Installer CD into your CD-ROM drive.
•
Wait a few moments for the auto-play function to run the set-up program
automatically. If the installation doesn’t start automatically, double click on
the executable file on the CD.
•
Follow the onscreen prompts to install the Infinity Camera software.
3. Infinity Camera Installation
i. After the software has been installed, plug your Infinity Camera into a free
USB 2.0 High-Speed port. For the Infinity X, power the camera using the
supplied wall power adapter.
ii.
The Window’s New Hardware Wizard will pop-up detecting a new “Infinity
Unconfigured Device”. Select Install the software automatically from
the options that are presented to you and click Next. A warning may
appear notifying you that the drivers have not been digitally signed by
Microsoft. Click Continue Anyway to continue with the driver installation.
This will not affect the camera’s performance. Then click Finish to
complete the installation of the drivers.
iii.
After a few seconds the Window’s New Hardware Wizard will pop-up
again (if it doesn’t, disconnect and reconnect the USB cable to the
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camera), detecting an “Infinity USB 2.0 Camera” device. Select Install
the software automatically from the options that are presented to you
and click Next. A warning may appear notifying you that the drivers have
not been digitally signed by Microsoft. Click Continue Anyway to continue
with the driver installation. Then click Finish to complete the installation of
the drivers.
Note: Windows will require you to re-run steps ii & iii each
time you plug the camera into a new USB 2.0 port. You
must have administrator privileges the first time the camera
is used on any given USB 2.0 port. You may wish to repeat
the installation steps at this time for all USB 2.0 ports on
your computer.
iv.
2.2
Run the application software from your Start menu and enjoy!
Technical Support
If you have problems installing or using the software, or with general camera
operation, please contact your place of purchase.
To obtain the latest software release and other technical information you may
also visit our technical support website at:
www.lumenera-scientific.com/support.htm
To register for automatic notification of new software releases you may send an
email to: upgrades@lumenera-scientific.com. You will be notified by email when
free software upgrades are available. Your privacy is important to us. Your
email address will not be used for any other purpose than to notify you of
available upgrades.
2.3
Installed Software
All of the necessary software and device drivers are contained in an installation
program on the CD-ROM that comes with the camera.
The following files are installed when you run the installation program:
Infinity Capture Application: Lumenera Scientific provides a free, easy to use
capture application with all of the features required for basic camera functionality
and image capture.
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Infinity TWAIN Interface: A plug-in driver will be installed by the installation
program that will allow all Infinity cameras to be used with any 3rd party software
application that supports a TWAIN device.
Infinity Direct Show Interface: Filter drivers will also be installed by the
installation program that will allow all Infinity cameras to be used with any 3rd
party software application that supports Direct Show capture devices.
2.4
The Infinity Camera
All Infinity cameras have a power jack and a high-speed USB port found on the
back of the camera. After powering the camera, simply plug it into a USB 2.0
(high-speed) compatible port and the camera will be functional. An LED light
between the power jack and USB port will indicate power is reaching the camera
and that the camera is running.
The Infinity 1 can be powered via the USB cable alone, which nominally supplies
5 volts. For this model, the power adapter is only required in cases where the
USB cable does not supply power (as is the case with some laptops.)
For all other Infinity models, the supplied power adapter should be used to power
the camera, as the power supplied by the USB bus is not sufficient for optimum
performance.
The power adapter supplied has the following specifications:
1. 6 Volts DC Regulated
2. 900 mA Minimum Current Rating
3. 2.1 mm tip, Center positive (+)
The camera is equipped with an industry standard C-Mount lens mount, which is
compatible with standard lenses or microscope mounts. In some cases, a Cmount adaptor may be required to optimize the field of view between your
microscope’s eyepieces and the image viewed on the computer monitor. Your
sales representative will recommend an adaptor to suit your requirements.
The Infinity X camera is unique in that it utilizes DeltaVU™ a patent-pending
technology that increases the resolution of captured images to as much as 21
megapixels (5120 x 4096) with true non-interpolated color at each pixel location.
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3
Using the Camera
3.1
Infinity Capture
Infinity Capture is a powerful and flexible yet simple software application for the
Live Preview, Optimization and Capture of digital images from any of the Infinity
cameras.
It is supported by Windows and has a graphical user interface with standard pull
down menus, an icon based toolbar and keyboard commands, which make it
very simple to control the camera and take images.
The following sections describe the usage of the software and explain the various
camera settings and how they relate to the changes viewed in the live and
captured images.
3.2
Launching the Application
To launch Infinity Capture from your Windows Start menu:
or
Select:
Programs > Infinity Camera > Infinity Capture.
(If you installed the software at a different location, the above instruction will be
different based on the location you chose.)
Only one camera may be controlled by each instance of Infinity Capture, but
several instances of the application may be run simultaneously. If more than one
camera is detected by the application, a list of cameras and their corresponding
serial numbers is displayed allowing you to select the camera you wish to control.
3.2.1 Main Window
The main window can be seen in Figure 1. When Infinity Capture starts up, the
program’s live preview window also starts. The main window has the toolbar with
the most common program actions available for quick access.
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Figure 1 - Infinity Camera Main Window
3.3
Selecting Image Size
3.3.1 Image Preview Size
You may choose any one of the available resolution settings from the dropdown
list on the main window for video preview as depicted in Figure 2. Depending on
the camera model, the available selections will be different. The default
resolution on start up is 640 x 480. The video frame rate displayed on your
monitor will vary depending on the choice of preview resolution. With each step
down in resolution, you will utilize a smaller area of the camera’s sensor (smaller
field of view) resulting in less video data and increased frame rates. The
exception to this is the sub-sampled mode, which utilizes the entire area of the
sensor (full field of view) but skip rows and columns of pixels to reduce the
amount of image data. Using this mode results in a megapixel image area at
higher frame rates but with less detail.
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Figure 2 - Image Preview Size
3.3.2 Capture Resolution
Image capture resolution is selected through the drop down list in the main
window similarly to the Image Preview Size. Capture resolution is independent
of preview resolution. This represents the resolution of the image that is
captured.
The Infinity X camera has 3 different DeltaVU™ resolutions available for each
preview resolution in addition to the standard (Demosaic) method used by all
other cameras.
3.4
Image Optimization
3.4.1 Exposure
Optimizing the exposure of your Infinity camera is achieved through moving the
exposure slider, Figure 3. Based on the camera model, you have different
exposure ranges available. Fine exposure slider adjustments can be made using
the left and right arrow keys on your keyboard. As exposure is adjusted, the
frame rate is kept at the maximum possible.
Some cameras have an extended exposure range, indicated by an Ext. check
box on the right side of the exposure slider. When not in extended exposure
mode, the exposure is limited but has high granularity so that very precise
exposure settings can be made. When the option is selected, the exposure can
be increased to the maximum allowed by the camera, but with less granularity.
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For the Infinity 2 only, the live video display will not show the extended exposure
(it will only show the maximum available when the extended option is not
selected), however, when you capture an image, the extended exposure will be
used for the capture.
Note: If you have image averaging turned on and are using
long exposures, you may have to wait a long time to capture
an image.
3.4.2 Gain
Adjusting the global electronic gain of your Infinity camera results in an increase
in sensitivity without increasing the exposure. The slider is under the exposure
slider on the main window as depicted in Figure 3. The global gain of each
Infinity camera model is optimized on start up. General image quality may
decrease as the gain is adjusted upward because the noise in the image is also
amplified along with the image signal. On some camera models such as Infinity
1, global gain is not adjustable and the slider will be disabled. In these cases,
individual RGB gains may still be adjusted. This feature is described under White
Balance.
Figure 3 - Exposure and Gain Control
3.4.3 Selecting the Light Source
Each light source emits light at a specific frequency resulting in a specific color
temperature. Color temperature measurement is typically referred to in degrees
Kelvin (K). Warmer tungsten / Halogen light sources have temperature in the
3000 K range. Fluorescent light sources have a temperature in the 4000 K range
and daylight conditions have cooler color temperatures in the 5500 K range.
Your Infinity camera is set to Tungsten / Halogen as a default. Selecting the light
source that best suits your working environment will help in optimizing your
images.
From the Options menu, you can select the light source as depicted in Figure 4.
Select Tungsten / Halogen for microscopy applications. When working in the
field, outdoor lighting conditions with a standard lens, selects the Daylight source.
When working indoors, office environment with fluorescent lighting, select the
Fluorescent source.
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If you are using filters on the light source, you should choose the light source that
best matches the filter. For example, on a microscope with Tungsten lighting, if
you use the standard daylight filter, you should select Daylight as the light
source.
Figure 4 - Selecting the Light Source
3.4.4 White Balance Target
Your Infinity color camera has the ability to adjust the colors in your image based on the
current lighting conditions. In order to adjust and accurately represent the colors within
your image, you must first perform a white balance.
As a first step, select a light source that accurately represents your working conditions,
described above. Next, select a target color that most accurately represents the current
working conditions. For a microscopy application this will be the background color when
viewing a sample through the eyepieces.
The default target color on start-up is white and in most cases this is the desired choice.
However, when viewing an image with the microscope eyepiece, especially when color
filters are being used, the background color is sometimes non-white. If you want the
colors captured by the camera to be as close as possible to the colors you see down
the eyepiece you should select a color target from the Available White Balance Target
Colors that closely matches the background color. The target color dialog can be
accessed by selecting the “bull’s eye” icon from the toolbar or from the dropdown menu
as depicted in Figure 5.
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Figure 5 - White Balance Target Dialog Access
You can select a target color, simply by clicking or dragging the mouse pointer in
the top window of the dialog depicted in Figure 6. The selected color appears in
the bottom window. Alternatively, you can select the Red, Green and Blue
values of the color.
Figure 6 - White Balance Target Dialog
3.4.5 White Balance
There are three methods available to adjust the white balance:
1. One Shot Global
This method balances the colors over the entire field of view. For best results,
move your sample out of the field of view. If you are using a transmitted light
microscope, move to a clear area of the slide. If operating a stereomicroscope,
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use the white base or a white piece of paper to represent the background.
Perform the One Shot Global White Balance function by selecting the Globe icon
from the toolbar, or by selecting One Shot Global from the Camera > White
Balance drop down menu as depicted in Figure 7.
Figure 7 - White Balance / One Shot Global
2. One Shot Spot
Under certain circumstances, users may wish to adjust white balance based on a
small area within the sample area. In these cases, One Shot Selection is the
method of choice. To access the One Shot White Balance, select the crosshair
icon from the toolbar, or select One Shot Spot from the camera drop down menu
as depicted in Figure 8.
Once selected, move the cursor to the area of your image that you want to white
balance (the mouse pointer changes to a cross hair when the mouse is in the
image preview window) and select it. The size of the spot on the image used for
the white balance is 32 x 32 pixels. You can cancel the operation prior to clicking
on the image display by reselecting the icon or menu item.
Figure 8 - White Balance / One Shot Spot
3. Adjust Manually
It is possible to manually adjust the color balance by changing the individual color
gains. This is helpful where slight adjustments to the white balance are required
or when the One Shot methods are unable to achieve a good balance. The
sliders that control the red, green and blue gains can be accessed via the
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tricolour icon on the toolbar or by selecting Adjust Manually from the Camera >
White Balance drop down menu as depicted in Figure 9.
Figure 9 - White Balance / Adjust Manually
Not all cameras have a green gain adjustment. In those cases, simply adjust the
Red and Blue gains together, higher to remove green and lower to add green.
The gain values represent multiplicative factors. The Initialize button will set all
gains to one and the Reset button will reset the gains to the values they had
when the dialog was popped up.
Figure 10 - Manual Gain Adjustment Dialog
Note: If you use an extreme white balance target color, a
proper white balance may not be achievable using the one
shot or manual methods. Likewise, under extreme lighting
conditions (very warm or very cool) proper white balance
may be difficult to achieve. In the latter case, try using a
color filter to bring the light temperature closer to the daylight
standard of 5500K.
3.4.6 Hue and Saturation
Hue is a term typically used to describe the tone in your image. Adjusting the
hue changes the appearance of the image by shifting the color spectrum
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incrementally. It’s not a substitute for white balance but can be used to give
images a warmer or cooler tone.
Saturation typically refers to the dominance of color intensity within your image.
A low saturation image looks dull and adding saturation can make the colors
more vibrant.
The dialog for Huw/Saturation adjustment can be accessed via the rainbow icon
on the toolbar or by selecting Hue/Saturation from the Camera drop down menu.
Figure 11 - Hue / Saturation Dialog
The sliders are used to adjust the hue and saturation, increasing when moved to
the right and decreasing when moved to the left. By moving the saturation slider
all the way to the left, all color is removed from the image making it appear
monochrome or grayscale. The Initialize button will set the Hue & Saturation to
zero and the Reset button will reset the Hue & Saturation to the values they had
when the dialog was popped up.
3.4.7
Contrast / Brightness / Gamma
The dialog for Contrast/Brightness/Gamma adjustment can be accessed via the
light bulb icon on the toolbar or by selecting Contrast/Brightness/Gamma from
the Camera drop down menu.
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Figure 12 - Contrast / Brightness / Gamma Dialog
Adjusting the contrast affects the brightness relationship between the dark and
light areas in your image. Adjusting the slider to the right increases contrast,
making dark areas darker, bright areas brighter and eliminating brightness levels
in between. Moving the slider to the left decreases contrast causing the
brightness of the image to become more uniform and flat.
Adjusting the brightness affects the overall image intensity. Moving the slider to
the right and left makes the image brighter and darker respectively.
Gamma adjustment affects the bright, dark and between areas of your image in a
non-linear fashion. The purpose of gamma is to correct the non-linearity in most
display devices. Gamma values greater than 1.0, will brighten the darker areas
of an image without over brightening the bright areas. For most monitors, a
good gamma value will be lie in the range 1.3 to 2.0.
The Initialize button will set the Contrast and Brightness to zero and the Gamma
to one. The Reset button will reset the Contrast, Brightness and Gamma to the
values they had when the dialog was popped up.
3.5
3.5.1
Capturing an Image
Capture Image
There are two steps to capture and save an image.
¾ First, select the Capture Image Icon to capture the image and display it on
screen.
¾ Next, select the Save Captured Image Icon to save it to disk.
These commands are also available from the drop down menu.
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Figure 13 - Capture and Save Image
¾ You have a choice of four file types to select from when saving images.
Note that JPEG images are compressed and there will be some loss of
image detail with that format. All other formats are uncompressed with no
loss of image detail.
Figure 14 - Image Saving File Formats
3.6
Other Options
3.6.1 Light Source Frequency
The light source frequency settings simply limit the available exposures that can
be used in order to avoid the flickering or banding caused by lights operating on
AC power sources. When using DC powered lights, as most microscopes have,
the DC setting may be selected and there will be no restriction on the allowable
exposures.
3.6.2 Preview Quality
The video displayed in the preview window can be optimized for either image
quality or speed. The default setting is for image quality, which uses a higher
percentage of the CPU and may result in a slightly slower video rate on some
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slower computers. When the “optimized for speed” option is selected, the image
quality is noticeably poorer, but CPU usage is minimized and frame rates are
always at their maximum. This setting does not affect how images are captured.
Captured images always use the highest quality algorithms to process the raw
data from the camera into full color images.
3.6.3 Preview Zoom Level
The preview window can be set to “Actual Size (100%)”, which will display the full
field of view at its actual size. Selecting “Half Size (50%)” will shrink the display
50% in each direction while maintaining the full field of view. This option is useful
when previewing large resolutions that are larger than the monitor’s display size.
3.6.4 Generate Flat Field Correction
Flat Field Correction is used to correct for uneven lighting and other fixed pattern
non-uniformity in the image. This action is used to generate the correction to be
applied to the image. Before selecting it, you should make all your imaging
adjustments (exposure, gain, etc.) and then remove any sample from the field of
view so that you only have an empty “background” image. Once the correction is
generated, it can be applied using the next action.
3.6.5 Enable Flat Field Correction
This action applies the flat field correction that was generated in the previous
action. When enabled, the correction will be automatically applied to both the
live preview and the captured image. If you change the resolution of the preview,
the correction will be reset and disabled. If you change the field of view, by
selecting a different objective lens, for example, you should re-generate the
correction for the new field of view.
3.6.6 Image Flipping
Image flipping (vertical) and mirroring (horizontal) are available from the Options
drop down menu or the left-right, up-down arrow icons on the toolbar. Selecting
these options will flip the preview image 180° either vertically or horizontally.
This option also affects the captured image.
3.6.7 Multiple Image Averaging
This feature is accessed through the Options drop down menu. Image averaging
is an effective tool to reduce random noise in your images. To enable this
feature, you select the Multiple Image Averaging option from the Options Menu.
You can change the number of images that are averaged together using the
Multiple Image Settings Dialog, which is accessed by selecting the Multiple
Image Settings option in the Options menu.
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When enabled, every time you capture an image the number of images you
selected will automatically be collected and averaged together.
This feature is not supported by the Infinity X when capturing high-resolution
(DeltaVU™) images.
3.6.8 Preview Histogram
A real-time intensity histogram can be overlaid on the preview window. This
histogram can be used as a guide to setting the image properties such that the
full dynamic range of the image sensor is being utilized. To display the preview
histogram, select Preview Histogram from the drop down Options Menu or select
the histogram icon from the toolbar.
3.6.9 Highlight Saturated Pixels
This option allows you to highlight the pixels in the image that are at or near
saturation. Saturation in this case refers to the brightness of the pixel. Starting
at a value of 240 (out of 255) the pixels will be changed to light red and as they
get closer to their saturated value of 255 will get progressively darker red. In the
case of a monochrome camera, the pixels will turn black, not red.
3.6.10 Load Overlay Bitmap
Any arbitrary bitmap can be overlaid on the live preview display or captured
image. A set of digital reticles is included with the application that can be used
for measurements or to aid object indexing and alignment.
Once the bitmap is loaded, you select from the Options Menu to overlay on the
preview image and/or the captured image.
You can also create your own bitmaps to overlay, using any paint program. The
bitmap must have a width that is evenly divisible by four and must be in RGB24
format, but other than that, there are no restrictions. Any part of the bitmap that
is pure white (RGB = 255, 255, 255) will be transparent with any other color
overlaying and replacing the image data.
If the bitmap is smaller than the image display resolution, the bitmap will be
placed in the upper left corner of the image. If either of the width or height of the
bitmap is larger than the image display resolution, the bitmap will be centered.
Currently, overlays are not supported for monochrome cameras.
3.6.11 Load Alpha Blending Bitmap
An alpha blending bitmap can be used whenever an overlay bitmap is being
used. This bitmap affects the way the overlay bitmap is actually overlaid. It is
often undesirable to have the bitmap completely obscure the image data. The
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alpha blending bitmap will cause the overlay to blend with the image data making
it less obtrusive. It can also be used to make a solid bitmap, like a logo for
example, into a watermark that can be used to tag images without hiding the
image underneath it.
The alpha bitmap has the same restrictions as the overlay bitmap, and must be
the same size as the overlay bitmap. The way it works is as follows: The value
of each pixel in the alpha bitmap indicates the level of transparency of the pixel at
the same location in the overlay bitmap. Pixels that have a value of 255 are
treated as completely transparent so that only the pixel from the original image is
used. Pixels that have a value of zero are treated as completely opaque so that
only the pixel from the overlay bitmap is used. The transparencies of the pixels
in-between vary linearly with their value. The blending is done independently for
each of the three color-channels (R, G & B), which allows for some interesting
effects, such as changing the color of the overlay, not just blending it with the
image.
The reticles bitmaps included with the application can be used as their own alpha
blending bitmaps. If you use any one of the gray reticles, they will simply blend
the colored reticles into the image to varying degrees. The dark gray will blend a
little bit, the gray will blend half and half and the light gray will blend a lot. For
interesting effects, try experimenting with using the color reticles as alpha
bitmaps.
Currently, overlays are not supported for monochrome cameras.
3.6.12 Load Settings / Save Settings / Save Settings As
At any time, you can save the current settings of the camera and application to a
file for later retrieval and use. When the application exits, it saves the current
settings to a file that is automatically loaded the next time it is run. With your own
settings files, you can quickly jump between different resolutions, exposures etc.
Save Settings, will save the settings to the currently loaded file (if it exists). Save
Settings As, allows you to use a different file.
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Using Infinity Cameras with TWAIN
Many programs support camera control and image capture directly using the
standard TWAIN protocol. Paint programs, Microsoft Office applications as well
as a wide variety of scientific software applications identify and run the Infinity
cameras through the supplied TWAIN interface.
The operation of the TWAIN interface is virtually identical to the Infinity Capture
application. The only difference is that when you capture an image, instead of
displaying in on screen, it passes it to the underlying TWAIN enabled application.
There is no facility to save images directly using the TWAIN interface.
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