Pressure Injection Cell Operator`s Manual

Pressure Injection Cell
Operator’s Manual
Operator’s Manual for the Pressure Injection Cell
Operator’s Manual
Pressure Injection Cell
CONGRATULATIONS!
Congratulations on your purchase of a
Next Advance Pressure Injection Cell.
Please read this operator’s manual which
explains proper operation of the instrument.
This manual can alsobe viewed on the web at
http://www.nextadvance.com/public/file/Press
_Inject_Cell_PC77_manual.pdf
Enjoy using your Pressure Injection Cell.
We’re sure that it will work well for you and
wish you good luck with your work.
CONTENTS
Parts of the Pressure Injection Cell............. 2
Setting Up Your Pressure Injection Cell..... 3
Using Your Pressure Injection Cell ............ 3
Stirplate Operation.......................................4
Maintenance................................................ 4
Disconnecting and Reconnecting the Unit.. 4
Troubleshooting .......................................... 5
Support........................................................ 5
Specifications.............................................. 5
Warranty ..................................................... 5
Safety Warning: Always close the valves on the pressure source and release any pressure from
inside the pressure injection cell before opening the cell (removing the top) or connecting or
disconnecting tubing to the cell. Never open the valves or try to pressurize the pressure injection
cell without all connections properly tightened and the top securely bolted to the base of the cell.
Wear protective eyewear. Users must be trained in working with high pressure systems and follow
proper safety procedures and regulations.
BE SAFE. ALWAYS MAKE SURE THAT THERE IS NO PRESSURE IN THE CELL BEFORE LOOSENING THE
BOLTS, AND THAT THE BOLTS ARE SECURELY FASTENED BEFORE APPLYING PRESSURE TO THE CELL.
USERS MUST ALWAYS WEAR PROTECTIVE APPAREL, INCLUDING EYEWEAR AND MUST BE TRAINED IN
WORKING WITH HIGH PRESSURE.
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Operator’s Manual for the Pressure Injection Cell
PARTS OF THE PC77-MAG PRESSURE INJECTION CELL
Bolt
Knob
Nut
Vent
Teflon Ferrule
(under the nut)
Top
Base
3-Way Valve
Inlet
Magnetic
Stirplate
SYMBOLS USED ON THE PC77- MAG MODEL WITH STIRPLATE
ON position of the Power Switch.
OFF position of the Power Switch
Caution: Follow the Instructions in the Operator’s Manual
This product complies with the European Low Voltage and EMC
Directives.
Please dispose the test tubes, capillary and the Pressure Cell in
accordance with local regulations
BE SAFE. ALWAYS MAKE SURE THAT THERE IS NO PRESSURE IN THE CELL BEFORE LOOSENING THE
BOLTS, AND THAT THE BOLTS ARE SECURELY FASTENED BEFORE APPLYING PRESSURE TO THE CELL.
USERS MUST ALWAYS WEAR PROTECTIVE APPAREL, INCLUDING EYEWEAR AND MUST BE TRAINED IN
WORKING WITH HIGH PRESSURE.
2
Operator’s Manual for the Pressure Injection Cell
SETTING UP YOUR PRESSURE
INJECTION CELL
Setting up the Pressure Injection Cell
consists of attaching it to 1/8 inch (outside
diameter) thick walled stainless steel tubing
(not supplied with the PC77 Pressure
Injection Cell). First, slide the tubing through
the inlet nut until it hits a stop. Finger tighten
the nut and then tighten it with a wrench for
1-1/4 turns. Connect the other end of the
tubing to a pressurized gas tank. It is now set
up.
USING YOUR PRESSURE
INJECTION CELL
OVERVIEW
Using the pressure injection cell requires
removing the top, inserting your sample,
replacing the top, and then inserting a
capillary. Next, inject the sample using
pressurized gas to force the sample through
the capillary. After the injection, close the gas
tank valve and open the 3-way valve to
release the pressure.
REMOVING THE TOP
With the valves at the pressure source
turned off and the 3-way valve opened to
release any pressure inside the cell, loosen the
three bolts securing the top, 2 turns each.
Rotate the top counterclockwise by 60
degrees so that the hexagonal sides are even.
Then lift off the top.
INSERTING THE SAMPLE
Place the sample tube in the main hole of
the base. The tube should not be capped. If
the cap is hinged, you must cut off the hinge
(with scissors). There is a recess in the top so
the tube can protrude from the base, but a
hinged cap will not fit in the recess.
The sample tube protrudes from the base for easy
insertion and removal.
REPLACING THE TOP
Confirm that the black O-ring is seated in
its groove. Slip the top onto the base, rotate
the top 60 degrees clockwise, and then
tighten the three bolts using a hex wrench.
Use a hex wrench to tighten the three bolts.
INSERTING A CAPILLARY
Loosen the nut covering the white ferrule
using a wrench. Slide the capillary through
the small hole in the ferrule, until it reaches
the bottom of the sample tube. Tighten the
nut, thereby squeezing the ferrule tightly
around the capillary.
To remove the capillary, loosen the nut and
slide the capillary out.
INJECTING A SAMPLE
First point the knob on the 3-way valve
towards the stainless steel tubing connected
to the gas tank. Then, while looking away
from the gas tank gages and with your hand
covering them (in case they explode when the
pressure is turned on) open the gas tank
valve. Adjust the pressure regulator to the
BE SAFE. ALWAYS MAKE SURE THAT THERE IS NO PRESSURE IN THE CELL BEFORE LOOSENING THE
BOLTS, AND THAT THE BOLTS ARE SECURELY FASTENED BEFORE APPLYING PRESSURE TO THE CELL.
USERS MUST ALWAYS WEAR PROTECTIVE APPAREL, INCLUDING EYEWEAR AND MUST BE TRAINED IN
WORKING WITH HIGH PRESSURE.
3
Operator’s Manual for the Pressure Injection Cell
desired pressure.
When the injection is finished, close the
gas tank valve. Then turn the knob on the
3-way valve so that it points toward the vent.
This depressurizes the injection cell. Now
you can loosen the nut over the ferrule and
remove the capillary, loosen the three bolts,
remove the top, and remove your sample
tube.
sample tubes will protrude above the surface,
making placement and retrieval easy. A
(surprisingly) simple adapter will enable 1 ml
microcentrifuge tubes to protrude, too. The
adapter is a piece of soft tubing (1/4 inch
inside diameter, 3/8 inch outside diameter).
As with any tube with a hinged cap, first cut
off the hinge so that the top of the tube will fit
in the recess in the top of the pressure
injection cell. Next, slide the adapter onto the
bottom of the 1 ml sample tube, as shown in
the figure below.
STIRPLATE OPERATION
(PC77-MAG model only)
Operation of the stirplate is straightforward.
The power supply should be plugged in to the
jack at the rear. The speed control knob is on
the left. Clockwise rotation increases the
speed. The power switch is on the right.
Use a wrench to tighten the nut, forcing the ferrule
to squeeze tightly around the capillary.
USE OF ADAPTER FOR 1 ML TUBES
We supply one adapter with the pressure
injection cell so that 1 ml tubes are as easy to
work with as taller tubes.
MAINTENANCE
Periodically, the Teflon ferrule should be
replaced. Simply remove the nut covering the
ferrule, remove the ferrule, place a new
ferrule, tapered side down into the fitting, and
replace the nut. Your pressure injection cell
comes with 10 ferrules (plus one installed in
the top). Additional ferrules can be purchased
directly from Chromatography Research
Supplies (part number 214204, 1/8” to 0.4mm
PTFE ferrules), at www.chromres.com
DISCONNECTING AND
RECONNECTING THE UNIT
Photograph of a 1 ml tube with a hinged cap (left)
and of a 1 ml tube with the hinge cut off and the
bottom placed in an adapter composed of soft
tubing (right).
The central hole in the base of the pressure
injection cell is sized so that 1.5 or 2 ml
Before you disconnect the pressure injection
cell, mark lines on the nut and the valve,
close the valves by the pressure source, and
release any pressure inside the cell by
opening the 3-way valve. When you reconnect the unit, you can use the same ferrule
and tubing. Simply tighten the nut slightly
more than last time. You should feel the
wrench get very hard to turn once you go
beyond the nut’s previous position.
BE SAFE. ALWAYS MAKE SURE THAT THERE IS NO PRESSURE IN THE CELL BEFORE LOOSENING THE
BOLTS, AND THAT THE BOLTS ARE SECURELY FASTENED BEFORE APPLYING PRESSURE TO THE CELL.
USERS MUST ALWAYS WEAR PROTECTIVE APPAREL, INCLUDING EYEWEAR AND MUST BE TRAINED IN
WORKING WITH HIGH PRESSURE.
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Operator’s Manual for the Pressure Injection Cell
TROUBLESHOOTING
SPECIFICATIONS
In addition to the tips given below, a
thorough list of troubleshooting tips is at
http://www.nextadvance.com/api/index.cfm/c
ms.page/i/5063/pressure-injection-cell-troubl
eshooting.
If gas leaks through the top-base
interface of the pressure injection cell, the
three bolts might not be fully tightened or the
O-ring might be nicked or dirty or missing. If
the O-ring is dirty, wash it off with a soap and
water and return it to its groove. Contact us
for a replacement O-ring.
If gas leaks around the capillary, the
ferrule should be replaced.
If you do not see sample traveling
through the capillary after a few minutes,
check that the gas tank is supplying the
desired pressure (typically on the order of
several hundred psi) and that the 3-way valve
knob is pointing toward the pressurized gas
line.
Max. Pressure: 17 MPa (2500 psi)
Size: 18 cm (6.5 in.) long x 10 cm (4 in.)
wide x 19 cm (8 in.) high
Weight: 1.6 kg (3.5 lbs.)
Relative Humidity: 5 - 90% non-condensing
Operating Temperature: 4 - 40°C
Altitude: <2000m
Storage Temperature: -40 to 50°C
Meets
requirements
Power Requirement (PC77-MAG model):
24V DC, 0.8 A
A thorough list of FAQs is at
http://www.nextadvance.com/api/index.cfm/p
roducts.info/c/427/Pressure-Injection-Cell-Ca
pillary-Loader#/FAQ.
If you cannot find a good answer there,
please contact customer service by email at
support@nextadvance.com or by telephone.
The warranty is void if the Product is
defective due to product accident, product
modification, exposure to radiation other than
for sterilization, connection to an improper
electrical supply, lack of proper maintenance,
contamination, improper installation or misuse.
If the product is used in a manner not specified
by the manufacturer, the protection provided by
the equipment may be impaired. The warranty
shall also not apply to defects arising from fire,
flood, lightning or other conditions unrelated to
correct operation of the Product.
Next Advance’s liability is limited, at the
company’s election, to (1) refund of the
original purchaser’s purchase price for the
Product (2) repair of the Product, or (3)
replacement of the Product or defective parts.
Evidence of purchase by the original purchaser
is required. Next Advance may also request
SUPPORT
WARRANTY
Next Advance warrants its Products against
defects in materials and workmanship for time
periods which vary according to the Product.
Within these time periods, Next Advance will
replace or repair, without charge to the original
purchaser, any part which is defective.
Pressure Injection Cells
Two years
BE SAFE. ALWAYS MAKE SURE THAT THERE IS NO PRESSURE IN THE CELL BEFORE LOOSENING THE
BOLTS, AND THAT THE BOLTS ARE SECURELY FASTENED BEFORE APPLYING PRESSURE TO THE CELL.
USERS MUST ALWAYS WEAR PROTECTIVE APPAREL, INCLUDING EYEWEAR AND MUST BE TRAINED IN
WORKING WITH HIGH PRESSURE.
5
Operator’s Manual for the Pressure Injection Cell
documentation of proper maintenance, if
applicable.
Next Advance makes no other warranty,
express or implied, with respect to its
Products. NEXT ADVANCE MAKES NO
WARRANTY RESPECTING THE MERCHANTABILITY OF THE PRODUCTS OR
THEIR SUITABILITY OR FITNESS FOR
ANY PARTICULAR PURPOSE OR USE.
Next Advance shall not be liable for, indirect,
special, incidental or consequential damages
of any nature. Any recovery for any claim
shall be limited to the original purchase price
for the product.
WARNINGS AND CAUTIONS
Read the user’s manual before operating.
For indoor use only.
Pollution Degree 2 per EN 61010-1.
Overvoltage Category II per EN 61010-1
OPERATOR’S RESPONSIBILITY
Provide proof of purchase and provide
normal care and maintenance.
BE SAFE. ALWAYS MAKE SURE THAT THERE IS NO PRESSURE IN THE CELL BEFORE LOOSENING THE
BOLTS, AND THAT THE BOLTS ARE SECURELY FASTENED BEFORE APPLYING PRESSURE TO THE CELL.
USERS MUST ALWAYS WEAR PROTECTIVE APPAREL, INCLUDING EYEWEAR AND MUST BE TRAINED IN
WORKING WITH HIGH PRESSURE.
6
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