NucleoMag® 96 RNA

Total RNA
Isolation
User Manual
NucleoMag 96 RNA
January 2010 / Rev. 02
MACHEREY-NAGEL
Total RNA Isolation
Table of contents
1
2
Components
4
1.1 Kit contents
4
1.2 Material to be supplied by user
5
Product description
6
2.1 The basic principle
6
2.2 Kit specifications
6
2.3 Magnetic separation systems
7
2.4 Adjusting the shaker settings
8
2.5 Handling of beads
8
2.6 Elution procedures
9
3
Storage conditions and preparation of working solutions
10
4
Safety instructions – risk and safety phrases
11
5
Procedure
13
5.1 General procedure
13
5.2 Protocol for the isolation of total RNA from cells or tissue
15
Appendix
18
6.1 Troubleshooting
18
6.2 Ordering information
20
6.3 Product use restriction / warranty
21
6
MACHEREY-NAGEL – 01 / 2010, Rev. 02
3
Total RNA Isolation
1
Components
1.1
Kit contents
NucleoMag 96 RNA
1x 96 preps
4 x 96 preps
744350.1
744350.4
NucleoMag B-Beads
3 ml
12 ml
Lysis Buffer MR1
50 ml
200 ml
Binding Buffer MR2
80 ml
320 ml
Wash Buffer MR3
80 ml
320 ml
Wash Buffer MR4
250 ml
1000 ml
Elution Buffer MR5**
25 ml
100 ml
1 vial
(107 mg / vial)
4 vials
(107 mg / vial)
3 vials (size D)
12 vials (size D)
Reaction Buffer for rDNase
35 ml
4 x 35 ml
RNase-free H2O
5 ml
20 ml
1
1
Cat. No.
Reducing Agent TCEP
rDNase, lyophilized*
User Manual
* For preparation of working solutions and storage conditions see section 3.
** Elution Buffer MR5: RNase-free water
4
MACHEREY-NAGEL – 01 / 2010, Rev. 02
Total RNA Isolation
1.2
Material to be supplied by user
Product
Cat. No.
Separation plate for magnetic beads separation,
e.g., Square-well Block (96-well block with
2.1 ml square-wells)
740481
740481.24
4
24
Lysis tubes for incubation of samples and
lysis,
e.g., Rack of Tubes Strips (1 set consists of
1 Rack, 12 Strips with 8 tubes (1.2 ml wells)
each, and 12 Cap Strips)
740477.4
740477.24
4 sets
24 sets
740486.24
24
740673
20
740951
1 set
Elution plate for collecting purified RNA,
e.g., Elution Plate, U-bottom (96-well 0.3 ml
microtiterplate with 300 µl u-bottom wells)
e.g., Elution Plate, Flat-bottom (96-well
0.3 ml microtiterplate with 300 µl flat-bottom
wells)
For use of kit on KingFisher 96 instrument:
KingFisher® 96 Accessory Kit B
(Square-well Blocks, Deep-well tip combs,
Elution Plates for 4 x 96 NucleoMag 96 RNA
preps using KingFisher® 96 platform)
MACHEREY-NAGEL – 01 / 2010, Rev. 02
Pack of
5
Total RNA Isolation
2
Product description
2.1
The basic principle
The NucleoMag 96 RNA procedure is based on the reversible adsorption of nucleic
acids to paramagnetic beads under appropriate buffer conditions. Sample lysis is
achieved by homogenization in a solution containing chaotropic ions. For the adjustment
of conditions under which nucleic acids bind to the paramagnetic beads Buffer MR2
and the NucleoMag B-Beads are added to the lysate. After magnetic separation the
paramagnetic beads are incubated with a recombinant DNase to remove co-purified
DNA. Following a RNA rebinding step contaminants and salts are removed using the
Wash Buffers MR3 and MR4. Residual ethanol from previous wash steps is removed
by air drying. Finally, highly pure RNA is eluted with Elution Buffer MR5* and the RNA
can directly be used for downstream applications. The NucleoMag 96 RNA kit can be
used either manually or automated on standard liquid handling instruments or automated magnetic separators.
2.2
Kit specifications
NucleoMag 96 RNA is designed for rapid manual and automated small-scale preparation of highly pure total RNA from tissue or cell samples. The kit is designed for use with
NucleoMag SEP magnetic separator plate (see ordering information) or other magnetic
separation systems (see section 2.3). Manual time for the preparation of 96 samples is
about 120 minutes. The purified RNA can be used directly as template for RT-PCR, or
any kind of enzymatic reactions.
Due to the recombinant DNase provided with the kit, eluted RNA is virtually DNA-free.
NucleoMag 96 RNA allows easy automation on common liquid handling instruments
or automated magnetic separators, for example Thermo Fisher Scientific KingFisher®
instruments. The actual processing time depends on the configuration of the instrument
and the magnetic separation system used. Typically, 96 samples can be purified in less
than 120 minutes using the NucleoMag SEP on automation platforms.
The kit provides reagents for the purification of up to 30 µg of pure RNA from suitable
samples. Depending on the elution volume used concentrations of 10 – 30 ng / µl can
be obtained.
NucleoMag 96 RNA can be processed completely at room temperature.
NucleoMag B-Beads are highly reactive superparamagnetic beads. The binding capacity is approx. 1 µg of RNA per 1 µl of NucleoMag-B-Bead Suspension. 1 µl of suspension contains 130 µg beads.
* Elution Buffer MR5: RNase-free water
6
MACHEREY-NAGEL – 01 / 2010, Rev. 02
Total RNA Isolation
2.3
Magnetic separation systems
For use of NucleoMag 96 RNA the use of the magnetic separator NucleoMag SEP is
recommended. Separation is carried out in a Square-well Block (see ordering information). The kit can also be used with other common separators. See suppliers ordering
information for suitable separation plates.
Magnetic separator
Separation plate or tube
NucleoMag SEP (MN Cat. No. 744900)
Square-well Block (MN Cat. No. 740670)
Tecan Te-MagS™
1.5 ml tubes without lid (Sarstedt)
Static magnetic pins
Separators with static magnetic pins, for example NucleoMag SEP (for manual use
and for use on liquid handling workstations): This type of separator is recommended in
combination with a suitable microplate shaker for optimal resuspension of the beads
during the washing and elution steps. Alternatively, beads can be resuspended in
the buffer by pipetting up and down several times. For fully-automated use on liquid
handling workstations a gripper tool is required, the plate is transferred to the magnetic
separator for separation of the beads and transferred to the shaker module for resuspension of the beads.
Movable magnetic systems
Separators with moving magnetic pins, for example Te-MagS™ (for automated use
only): Magnetic pins / rods are moved from one side of the well to the other and vice
versa. Beads follow this movement and are thus pulled through the buffer during the
wash and elution steps. Separation takes place when the system stops.
Automated separators
Separators with moving magnets, for example Thermo Fisher Scientific KingFisher®
instruments: Magnetic beads are transferred into suitable plates or tubes. Beads are
resuspended from the rod-covered magnets. Following binding, washing or elution
beads are collected again with the rod-covered magnets and transferred to the next
plate or tube.
MACHEREY-NAGEL – 01 / 2010, Rev. 02
7
Total RNA Isolation
2.4
Adjusting the shaker settings
When using a plate shaker for the washing and elution steps the speed settings have
to be adjusted carefully for each specific separation plate and shaker to prevent crosscontamination from well to well. Proceed as follows:
Adjusting shaker speed for wash steps:
•
Load 900 µl dyed water to the wells of the separation plate. Place the plate on
the shaker and start shaking with a moderate speed setting for 30 seconds.
Turn off the shaker and check plate surface for small droplets of dyed water.
•
Increase speed setting, shake for an additional 30 seconds, and check plate
surface for droplets again.
•
Continue increasing the speed setting until you observe droplets on top of the
separation plate. Reduce speed setting, check again and use this setting for
the washing step.
Adjusting shaker speed for the elution step:
•
2.5
Load 100 µl dyed water to the wells of the collection plate and proceed as described above.
Handling of beads
Distribution of beads
A homogeneous distribution of the magnetic beads to the individual wells of the separation plate is essential for a high well-to-well consistency. Therefore, before distributing
the beads make sure that the beads are completely resuspended. Shake the storage
bottle well or place it on a vortexer shortly. Premixing magnetic beads with the binding
buffer allows easier homogenous distribution of the beads to the individual wells of the
separation plate. During automation, a premix step before aspirating the beads / binding
buffer mixture from the reservoir is recommended to keep the beads resuspended.
Magnetic separation time
Attraction of the magnetic beads to the magnetic pins depends on the magnetic
strength of the magnetic pins, the selected separation plate, distance of the separation
plate from the magnetic pins, and the volume to be processed. The individual times for
complete attraction of the beads to the magnetic pins should be checked and adjusted
on each system. It is recommended to use the separation plates or tubes specified by
the supplier of the magnetic separator.
Washing the beads
Washing the beads can be achieved by shaking or mixing. In contrast to mixing by
pipetting up and down mixing by shaker or magnetic mixing allows simultaneous mixing
of all samples. This reduces the time and number of tips needed for the preparation.
Resuspension by pipetting up and down, however, is more efficient than mixing by a
shaker or magnetic mix.
8
MACHEREY-NAGEL – 01 / 2010, Rev. 02
Total RNA Isolation
Method
Resuspension
efficiency
Speed
Number of tips
needed
Magnetic mix
+
++
Low
Shaker
++
++
Low
Pipetting
+++
+*
High
* 8-channel pipetting device
2.6
Elution procedures
Purified total RNA can be eluted directly with the supplied Elution Buffer MR5. Elution
can be carried out in a volume of ≥ 50 µl. It is essential to cover the NucleoMag B-Beads
completely with Elution Buffer MR5 during the elution step. The volume of dispensed
Elution Buffer MR5 depends on the magnetic separation system (e.g., the position of
the pellet inside the separation plate). For efficient elution the magnetic bead pellet
should be resuspended completely in the Elution Buffer MR5. For some separators
high elution volumes might be necessary to cover the whole magnetic bead pellet.
MACHEREY-NAGEL – 01 / 2010, Rev. 02
9
Total RNA Isolation
3
Storage conditions and preparation of
working solutions
Attention:
Buffers MR1 and MR3 contain chaotropic salt! Wear gloves and goggles!
•
All components of the NucleoMag 96 RNA kit should be stored at room temperature (20 – 25 °C) and are stable for up to one year.
•
All buffers are delivered ready-to-use.
Before starting NucleoMag 96 RNA protocol prepare the following:
•
rDNase working solution: Add 800 µl of RNase-free H2O to each rDNase vial
and incubate for 1 min at room temperature. Gently swirl the vials to completely
dissolve the rDNase. Be careful not to mix rDNase vigorously as rDNase is sensitive to mechanical agitation. If not used completely this working solution can
be stored at - 20 °C for up to 6 month. Do not freeze / thaw the rDNase working
solution more than three times.
•
rDNase reaction mixture: Add 9.2 ml Reaction Buffer for rDNase to 800 µl
rDNase working solution and mix. The resulting rDNase reaction mixture will be
sufficient for 32 isolations and should be used up. When performing less than
32 reactions prepare a smaller amount of the reaction mixture. For each isolation combine 276 µl of reaction buffer for rDNase with 24 µl of rDNase working
solution.
•
Reducing Agent TCEP: Add 750 µl of RNase-free H2O to the TCEP vial and
incubate for several minutes at room temperature. Mix the vial to dissolve the
TCEP completely. Store dissolved TCEP at -20°C.
NucleoMag 96 RNA
Cat. No.
rDNase (lyophilized)
TCEP
10
1 x 96 preps
4 x 96 preps
744350.1
744350.4
3 vials (size D)
12 vials (size D)
Add 800 µl RNase-free H2O
to each vial
Add 800 µl RNase-free H2O
to each vial
1 vial (107 mg)
4 vials (107 mg / vial)
Add 750 µl RNase-free H2O
Add 750 µl RNase-free H2O
to each vial
MACHEREY-NAGEL – 01 / 2010, Rev. 02
Total RNA Isolation
4
Safety instructions – risk and safety phrases
The following components of the NucleoMag 96 RNA kits contain hazardous contents.
Wear gloves and goggles and follow the safety instructions given in this section.
Component
Hazard
contents
Hazard
symbol
MR1
Guanidine
thiocyanate
Xn*
MR2
Isopropanol
> 90 %
F
Xn**
Risk
phrases
Safety
phrases
Harmful by inhalation,
in contact with the
skin, and if swallowed
R
20/21/22
S 13
Highly flammable
- Irritating to eyes
- Vapours may cause
drowsiness and dizziness
R 11-3667
S 7-1624-25-26
MR3
Guanidine
thiocyanate +
ethanol < 40 %
Xn*
Flammable - Harmful
by inhalation, in
contact with the skin,
and if swallowed
R 1020/21/22
S 13-16
MR4
Ethanol < 80 %
F**
Highly flammable
R11
S 7-16
rDNase,
RNase-free
rDNase,
lyophilized
Xi**
May cause sensitization by inhalation and
skin contact
R 42/43
S 22-24
Reducing
Agent TCEP
Tris (2-carboxylethylphosphine
Hydrochloride)
Xi**
Causes burns
R 34
S 26-2736/37/39
Risk phrases
R 10
Flammable
R 11
Highly flammable
R 20/21/22
Harmful if swallowed
R 34
Causes burns
R 36
Irritating to eyes
R 42/43
May cause sensitization by inhalation and skin contact
R 67
Vapours may cause drowsiness and dizziness
* Hazard labeling not necessary if quantity per bottle below 125 g or ml (certificate of exemption
according to 67/548/EEC Art. 25, 1999/45/EC Art. 12 and German GefStoffV § 20 (3) and TRGS 200 7.1).
For further information see Material Safety Data Sheet.
** Hazard labeling not necessary if quantity per bottle below 25 g or ml (certificate of exemption
according to 67/548/EEC Art. 25, 1999/45/EC Art. 12 and German GefStoffV § 20 (3) and TRGS 200 7.1).
For further information see Material Safety Data Sheet.
MACHEREY-NAGEL – 01 / 2010, Rev. 02
11
Total RNA Isolation
Safety phrases
S7
Keep container tightly closed
S 13
Keep away from food, drink, and animal feedstuffs
S 16
Keep away from sources of ignition - No smoking!
S 22
Do not breathe dust
S 24
Avoid contact with the skin
S 24/25
Avoid contact with skin and eyes
S 26
In case of contact with eyes, rinse immediately with plenty of water and seek
medical advice
S 27
Take off immediately all contaminated clothing
S 36/37/38
Wear suitable protective clothing and gloves
12
MACHEREY-NAGEL – 01 / 2010, Rev. 02
NucleoMag 96 RNA
5
Procedure
5.1
General procedure
1
Homogenize / lyse
sample
Up to 20 mg tissue
or 2 x 106 cells
350 µl MR1
6 µl TCEP
Mix or use
mechanical disruption
2
Bind RNA to
NucleoMag B-Beads
28 µl B-Beads
350 µl MR2
Mix
Incubate 5 min at RT
(Optional: Mix by shaking)
Remove supernatant
after 2 min separation
Dry for 5 min at RT
3
rDNase digestion
300 µl rDNase
reaction mixture
Mix
Incubate 15 min at RT
4
Rebinding
350 µl MR2
Mix
Incubate 5 min at RT
Remove supernatant
after 2 min separation
MACHEREY-NAGEL – 01 / 2010, Rev. 02
13
NucleoMag 96 RNA
5
MR3 wash
600 µl MR3
Resuspend,
separate, 2 min
Aspirate and discard
supernatant
6
MR4 wash
900 µl MR4
Resuspend,
separate, 2 min
Aspirate and discard
supernatant
Repeat washing step
once
7
Drying
8
Elution
Incubate for 10 – 15 min at RT
50 – 200 µl MR5
Shake 5 – 10 min, RT
(Optional: Mix by pipetting
up and down)
Transfer eluted RNA
after 2 min separation
14
MACHEREY-NAGEL – 01 / 2010, Rev. 02
NucleoMag 96 RNA
5.2
Protocol for the isolation of total RNA from cells or
tissue
This protocol is designed for magnetic separators with static pins (e.g., NucleoMag
SEP) and suitable plate shakers. It is recommended using a Square-well Block for
separation (see ordering information). Alternatively isolation of RNA can be performed
in reaction tubes with suitable magnetic separators. This protocol is for manual use and
serves as a guideline for adapting the kit to robotic instruments.
1
Homogenize / lyse sample
Lyse up to 20 mg of tissue or 2 x 106 cells in 350 µl Buffer MR1.
For tissue samples: Use a suitable homogenization tool to homogenize samples
in Buffer MR1. Samples can be disrupted using bead based homogenization
tools, for example GenoGrinder* or Mixer Mill MM400** (see instrument manufacturer’s recommendations for suitable plates or tubes for homogenization) or
any other suitable homogenization tools. Centrifuge the crude homogenate to
pellet debris or remaining tissue particles. Alternatively NucleoSpin® RNA Filter
Tubes or Plates can be used to clear the crude lysate (see ordering information). Transfer the clear supernatant to the Square-well Block (see ordering
information) for further processing.
For cells: Add Buffer MR1 to cell pellet. Pipette up and down several times to
lyse the cells. Optionally: Use NucleoSpin® RNA Filter Tubes or Plates (see
ordering information) or a syringe to reduce the viscosity of the lysate. Transfer
lysate to the Square-well Block for further processing.
2
Bind RNA to NucleoMag B-Beads
Add 28 µl resuspended NucleoMag B-Beads and 350 µl Buffer MR2 to the
lysed sample. Mix by pipetting up and down 6 times and incubate for 5 min at
room temperature. NucleoMag B-Beads and Buffer MR2 can be premixed.
Be sure to resuspend the NucleoMag B-Beads before removing them from the storage
bottle. Vortex storage bottle briefly until a homogenous suspension has formed.
Separate the magnetic beads against the side of the wells by placing the Squarewell Block on the NucleoMag SEP magnetic separator. Wait at least 2 min until
all the beads have been attracted to the magnets. Remove and discard supernatant by pipetting. Remove supernatant completely.
Do not disturb the attracted beads while aspirating the supernatant.
* GenoGrinder: http://www.spexcsp.com/sampleprep/
** Mixer Mill MM400 http://www retsch.com/products/milling/ball-mills/mm-400/
MACHEREY-NAGEL – 01 / 2010, Rev. 02
15
NucleoMag 96 RNA
Dry beads for 5 min at room temperature. Keep the Square-well Block on the
NucleoMag SEP magnetic separator for the drying step.
3
rDNase digestion
Remove the Square-well Block from the NucleoMag SEP magnetic separator.
Add 300 µl rDNase reaction mixture and resuspend the beads by pipetting
up and down. Incubate for 15 min at room temperature. Do not separate the
beads! Following incubation proceed with step 4.
4
Rebinding
Add 350 µl Buffer MR2 to each sample. Mix by pipetting up and down 6 times
and incubate for 5 min at room temperature.
Separate the magnetic beads against the side of the wells by placing the
Square-well Block on NucleoMag Sep magnetic separator. Wait at least 2 min
until all the beads have been attracted to the magnets. Remove and discard
supernatant by pipetting.
5
MR3 wash
Remove the Square-well Block from the NucleoMag SEP magnetic separator. Add 600 µl Buffer MR3 resuspend the beads by pipetting up and down.
Incubate for 1 min.
Separate the magnetic beads by placing the Square-well Block on the
NucleoMag SEP magnetic separator. Wait at least 2 min until all the beads
have been attracted to the magnets. Remove and discard supernatant by pipetting.
6
MR4 wash
Remove the Square-well Block from the NucleoMag SEP magnetic separator.
Add 900 µl Buffer MR4 and resuspend the beads by pipetting up and down.
Incubate for 1 min.
Separate the magnetic beads by placing the Square-well Block on the
NucleoMag SEP magnetic separator. Wait at least 2 min until all the beads
have been attracted to the magnets. Remove and discard supernatant by pipetting.
Repeat washing step once with 900 µl Buffer MR4. Leave the Square-well
Block on the NucleoMag SEP magnetic separator for the following step.
16
MACHEREY-NAGEL – 01 / 2010, Rev. 02
NucleoMag 96 RNA
7
Drying
Air dry the beads for 10 – 15 min at room temperature.
8
Elution
Remove the Square-well Block from the NucleoMag SEP magnetic separator.
Add desired volume of Buffer MR5 (at least 50 µl, 50 – 200 µl) and resuspend
the beads by pipetting up and down.
Incubate the suspension for 5 min at room temperature.
Separate the magnetic beads by placing the Square-well Block on the
NucleoMag SEP magnetic separator. Wait at least 2 min until all the beads
have been attracted to the magnets. Transfer the supernatant containing the
purified total RNA to a suitable collection plate (see ordering information).
MACHEREY-NAGEL – 01 / 2010, Rev. 02
17
Total RNA Isolation
6
Appendix
6.1
Troubleshooting
Problem
Possible cause and suggestions
RNA is
degraded /
no RNA
obtained
•
RNase contamination
Create an RNase-free working environment. Wear gloves
during all steps of the procedure. Change gloves frequently.
Use of sterile, disposable polypropylene tubes or plates is
recommended. Glassware should be oven-baked for at least
2 h at 250 °C before use.
Elution buffer volume insufficient
•
Beads pellet must be covered completely with elution buffer.
Insufficient performance of elution buffer during elution step.
•
Remove residual buffers during the separation steps completely. Remaining buffers decrease the efficiency of following wash
and elution steps.
Beads dried out
Poor RNA
yield
•
Do not let the beads dry as this might result in lower elution efficiencies.
Aspiration of attracted bead pellet
•
Do not disturb the attracted beads while aspirating the supernatant, especially when the magnetic bead pellet is not visible
in the lysate.
Aspiration and loss of beads
•
Time for magnetic separation too short or aspiration speed too
high.
Insufficient washing procedure
•
Use only the appropriate combinations of separator and plate,
for example Square-well Block in combination with NucleoMag
SEP.
•
Make sure that beads are resuspended completely during the
washing procedure. If shaking is not sufficient to resuspend the
beads completely mix by repeated pipetting up and down.
Low purity
18
MACHEREY-NAGEL – 01 / 2010, Rev. 02
Total RNA Isolation
Problem
Poor performance
of RNA in
downstream
applications
Possible cause and suggestions
Carry-over of ethanol from wash buffers
•
Be sure to remove all of the ethanolic wash solution, as residual
ethanol interferes with downstream applications.
Low purity
•
See above.
Time for magnetic separation too short
•
Carry-over
of beads
Increase separation time to allow the beads to be completely
attracted to the magnetic pins before aspirating any liquid from
the well.
Aspiration speed too high (elution step)
•
High aspiration speed during the elution step may cause bead
carry-over. Reduce aspiration speed for elution step.
Contamination of the rims
Cross contamination
•
Do not moisten the rims of the Square-well Block when transferring the tissue lysate. If the rim of the wells is contaminated, seal
the Square-well Block with Self-adhering PE Foil (see ordering
information) before starting the shaker.
MACHEREY-NAGEL – 01 / 2010, Rev. 02
19
Total RNA Isolation
6.2
Ordering information
Product
Cat. No.
Pack of
NucleoMag 96 RNA
744350.1
744350.4
1 x 96 preps
4 x 96 preps
NucleoSpin® Filters
740606
50
NucleoSpin® RNA Filter Plates
740711
4
NucleoMag SEP
744900
1
Square-well Blocks
740481.4
740481.24
4
24
Self-adhering PE Foil
740676
Rack of Tube Strips
(set consists of 1 Rack, 12 Tube Strips
with 8 tubes each and Cap Strips)
740477.4
740477.24
50 sheets
4 sets
24 sets
Cap Strips
740638
30 strips
KingFisher® 96 Accessory Kit B
Square-well Blocks, Deep-well tip
combs, Elution Plates for 4 x 96
NucleoMag 96 RNA preps using
KingFisher® 96 platform
744951
1 set
Visit www.mn-net.com for more detailed product information.
20
MACHEREY-NAGEL – 01 / 2010, Rev. 02
Total RNA Isolation
6.3
Product use restriction / warranty
NucleoMag 96 RNA kit components were developed, designed, distributed, and sold
FOR RESEARCH PURPOSES ONLY. They are suitable FOR IN - VITRO USES ONLY.
No claim or representation is intended for its use to identify any specific organism or for
clinical use (diagnostic, prognostic, therapeutic, or blood banking).
It is rather the responsibility of the user to verify the use of the NucleoMag 96 RNA
kit for a specific application range as the performance characteristic of this kit has not
been verified to a specific organism.
This MACHEREY-NAGEL product is shipped with documentation stating specifications and other technical information. MACHEREY-NAGEL warrants to meet the stated
specifications. MACHEREY-NAGEL´s sole obligation and the customer´s sole remedy
is limited to replacement of products free of charge in the event products fail to perform
as warranted. Supplementary reference is made to the general business terms and
conditions of MACHEREY-NAGEL, which are printed on the price list. Please contact
us if you wish an extra copy.
MACHEREY-NAGEL does not warrant against damages or defects arising in shipping
and handling (transport insurance for customers excluded), or out of accident or improper or abnormal use of this product; against defects in products or components not
manufactured by MACHEREY-NAGEL, or against damages resulting from such nonMACHEREY-NAGEL components or products.
MACHEREY-NAGEL makes no other warranty of any kind whatsoever, and
SPECIFICALLY DISCLAIMS AND EXCLUDES ALL OTHER WARRANTIES OF
ANY KIND OR NATURE WHATSOEVER, DIRECTLY OR INDIRECTLY, EXPRESS
OR IMPLIED, INCLUDING, WITHOUT LIMITATION, AS TO THE SUITABILITY,
REPRODUCTIVITY, DURABILITY, FITNESS FOR A PARTICULAR PURPOSE OR
USE, MERCHANTABILITY, CONDITION, OR ANY OTHER MATTER WITH RESPECT
TO MACHEREY-NAGEL PRODUCTS.
In no event shall MACHEREY-NAGEL be liable for claims for any other damages,
whether direct, indirect, incidental, compensatory, foreseeable, consequential, or special (including but not limited to loss of use, revenue or profit), whether based upon
warranty, contract, tort (including negligence) or strict liability arising in connection with
the sale or the failure of MACHEREY-NAGEL products to perform in accordance with
the stated specifications. This warranty is exclusive and MACHEREY-NAGEL makes
no other warranty expressed or implied.
The warranty provided herein and the data, specifications and descriptions of this
MACHEREY-NAGEL product appearing in MACHEREY-NAGEL published catalogues
and product literature are MACHEREY-NAGEL´s sole representations concerning
the product and warranty. No other statements or representations, written or oral, by
MACHEREY-NAGEL´s employees, agent or representatives, except written statements signed by a duly authorized officer of MACHEREY-NAGEL are authorized; they
should not be relied upon by the customer and are not a part of the contract of sale or
of this warranty.
MACHEREY-NAGEL – 01 / 2010, Rev. 02
21
Total RNA Isolation
Product claims are subject to change. Therefore please contact our Technical Service
Team for the most up-to-date information on MACHEREY-NAGEL products. You
may also contact your local distributor for general scientific information. Applications
mentioned in MACHEREY-NAGEL literature are provided for informational purposes
only. MACHEREY-NAGEL does not warrant that all applications have been tested in
MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREYNAGEL does not warrant the correctness of any of those applications.
Please contact:
MACHEREY-NAGEL Germany
Tel.: +49 (0) 24 21 969 270
e-mail: TECH-BIO@mn-net.com
Last updated: 12 / 2006, Rev. 02
Trademarks:
KingFisher is a registered trademark of Thermo Fisher Scientific
NucleoMag is a trademark of MACHEREY-NAGEL GmbH & Co KG
Te-MagS is a trademark of Tecan Group Ltd.
All used names and denotations can be brands, trademarks, or registered labels of their respective
owner – also if they are not special denotation. To mention products and brands is only a kind of
information (i.e., it does not offend against trademarks and brands and can not be seen as a kind
of recommendation or assessment). Regarding these products or services we can not grant any
guarantees regarding selection, efficiency, or operation.
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MACHEREY-NAGEL – 01 / 2010, Rev. 02
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