CryoMed Controlled-Rate Freezers - User Manual

Model 7452 Series
CryoMed Controlled Rate Freezer
Operating and Maintenance Manual 7007452 Rev. 11
Preface
Non-IVF Units
IVF Units
Model
Voltage
Model
Voltage
7450
120
7456
120
7451
230
7457
230
7452
120
7458
120
7453
230
7459
230
7454
120
7475
100
7455
230
7476
100
7472
100
7473
100
7474
100
Software Addendum
1. Installation of the National Instruments LabView Run Time Engine
is required for proper operation of the PC Interface Software.
2. A PDF version of the Windows Help file for the PC Interface
Software is located on the CD-ROM. The file name is
“Help_PC_Interface.pdf ”.
3. A “Read Me” file containing the known issues with Version 1.0 of the
PC Interface Software is located on the CD-ROM. The file name is
ReadMe.txt.
4. The Windows Help file can be accessed in the PC Interface Software
Edit, Run, Review or Options screens by depressing the F1 key on
the PC keyboard.
5. If password protection is enabled, note that user names and
passwords are case sensitive.
MANUAL NUMBER 7007452
11
25334
2/24/09
Removed LN2 Supply, pg 2-14
ccs
10
24349/FR-1980
3/26/08
New power supply with power cord 1950135
ccs
9
24391
1/21/08
Added PC operating system specs to Section 10
ccs
8
24326/SI-9956
12/19/07
Removed all IQ/OQ kits (obsolete)
ccs
7
23738/FR-1928
10/03/06
Corrected the calibration procedure for a recorder (Pg 3-1)
ccs
6
22854/FR-1860
5/11/05
Removed registration sheet, added password and data tag info
ccs
--
21878/FR-1735
9/16/03
Replaced P/N 103113 with 195986, 195987, 195988 SRO
ccs
5
21196/FR-1670
1/20/03
Add bend to bottom of probe guide tube
ccs
--
21141/FR-1670
1/20/03
Removed 4000714 kit, added 180254 cane holder and 4000211 cane
ccs
--
21280/FR-1670
1/20/03
Changed bottom hinge block to 190612 for baseline alignment
ccs
4
21059/FR-1642
10/9/02
Changed hinge block to 1900179 for better door alignment
ccs
--
--
6/14/02
Added power disconnect warning section 4.1 per S. Neill
aks
3
--
5/15/02
Received FDA clearance for use in human research
ccs
--
--
4/12/02
Added weight chart and new declarations of conformity for IVF units
ccs
--
20558/FR-1582
3/18/02
Updated schematics and wiring diagrams for external IVF connector/probe
ccs
2
20604/FR-1592
3/12/02
Updated schematics and wiring diagrams
aks
--
--
12/21/01
Added IVF models
ccs
1
--
11/12/01
Updated profiles
ccs
--
20382/FR1555
11/13/01
Change hinge mounting screws for freezer door
aks
0
200164/SI-8280
8/23/01
Original manual
ccs
REV
ECR/ECN
DATE
DESCRIPTION
By
Thermo Scientific
7007452 CryoMed Freezer
i
Preface
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. ▲
Caution All internal adjustments and maintenance must be performed by qualified service personnel. ▲
Material in this manual is for information purposes only. The contents and the product it describes are subject to
change without notice. Thermo Scientific makes no representations or warranties with respect to this manual. In
no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to the use of
this manual.
©2001 Thermo Scientific. All rights reserved.
ii
7007452 CryoMed Freezer
Thermo Scientific
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.
Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Scientific
has contracted with one or more recycling/disposal companies in each EU Member State European
Country, and this product should be disposed of or recycled through them. Further information on
Thermo’s compliance with this directive, the recyclers in your country and information on Thermo
products will be available at www.thermo.com.
✔
Always use the proper protective equipment (clothing, gloves, goggles, etc.)
✔ Always dissipate extreme cold or heat and wear protective clothing.
✔ Always follow good hygiene practices.
✔ Each individual is responsible for his or her own safety.
Thermo Scientific
7007452 CryoMed Freezer
iii
Preface
Do You Need Information or Assistance on
Thermo Scientific Products?
If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:
1-740-373-4763
1-800-438-4851
1-740-373-4189
http://www.thermo.com
service.led.marietta@thermofisher.com
Direct
Toll Free, U.S. and Canada
FAX
Internet Worldwide Web Home Page
Service E-Mail Address
Our Sales Support staff can provide information on pricing and give you quotations. We can
take your order and provide delivery information on major equipment items or make
arrangements to have your local sales representative contact you. Our products are listed on the
Internet and we can be contacted through our Internet home page.
Our Service Support staff can supply technical information about proper setup, operation or
troubleshooting of your equipment. We can fill your needs for spare or replacement parts or
provide you with on-site service. We can also provide you with a quotation on our Extended
Warranty for your Thermo Scientific products.
Whatever Thermo Scientific products you need or use, we will be happy to discuss your
applications. If you are experiencing technical problems, working together, we will help you
locate the problem and, chances are, correct it yourself...over the telephone without a service
call.
When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is
covered by the warranty, we will arrange for the unit to be repaired at our expense and to your
satisfaction.
Regardless of your needs, our professional telephone technicians are available to assist you
Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by
telephone or fax. If you wish to write, our mailing address is:
Thermo Scientific
Controlled Environment Equipment
401 Millcreek Road, Box 649
Marietta, OH 45750
International customers, please contact your local Thermo Scientific distributor.
iv
7007452 CryoMed Freezer
Thermo Scientific
Table of Contents
Section 1
Section 2
Thermo Scientific
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Standard Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
Door Latch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
Sample ‘Seeding’ Access Ports (IVF) . . . . . . . . . . . . . . . . . . . . . . . .1-2
Displays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Keys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Output . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Pre-set Profiles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Set-Up and Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3
Electrical Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3
Environmental Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3
Sample Thermocouple Connection . . . . . . . . . . . . . . . . . . . . . . . . . .2-4
Power-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-4
Access Code Security . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-5
Initiate a Profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-5
Stop a Profile Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-6
Manual Override . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-7
Program User Specific Profiles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-7
Copy and Paste Pre-set and User Profiles . . . . . . . . . . . . . . . . . . . . .2-10
Recorder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-11
Printer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-11
Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-12
Chamber Thermocouple Open . . . . . . . . . . . . . . . . . . . . . . . . . .2-12
Sample Thermocouple Open . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-13
Heater Malfunction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-14
Chamber/Sample Over/Under Limit Temp . . . . . . . . . . . . . . . . . .2-15
Power Failure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-17
Run Complete . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-17
Warm Complete . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-18
Seeding Hold . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-18
Printer Buffer Full . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-18
IVF Sample Holders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-18
Using a Vial Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-19
Using the Straw Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-19
Using the Cane Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-20
Holder Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-21
7007452 CryoMed Freezer
v
Table of Contents
Section 3
Section 4
Section 5
Section 6
Section 7
Section 8
Section 9
Section 10
vi
7007452 CryoMed Freezer
Configuration/Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Menu Structure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Menu Entry Definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Disinfect Controlled Rate Freezer Interior . . . . . . . . . . . . . . . . . . . . .4-1
Clean a Major Spill in the Chamber . . . . . . . . . . . . . . . . . . . . . . . .4-2
Clean the Cabinet Exterior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
Clean Gasket/Heat Break . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
Printer Set-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Change the Paper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Exploded Parts Drawings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-3
Wiring Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-15
Electrical Schematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1
PC Interface Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-1
Installation and Set-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-2
Main Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-2
Edit Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-3
Create a New Freezer Profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-7
Edit an Existing Profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-8
Print a Profile Listing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-8
Display Profile Temp Graph . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-9
Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-9
Run Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-10
User Control Definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-10
Status Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-13
Operating Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-13
Typical Run Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-15
Power Failure Recovery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-16
Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-17
Display/Control Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-18
Remote Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-18
Stand Alone Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-19
Running a Local Profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-19
Review Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-19
Information Fields . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-19
User Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-20
Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-23
Plot Color Selections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-23
Information Field Set-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-24
Default Directories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-24
User Name and Password Maintenance . . . . . . . . . . . . . . . . . . . .10-25
Saving Changes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-26
Sharing Host PC Resources . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-26
Thermo Scientific
Section 1
Introduction
Figure 1-1. Non-IVF Controlled Rate Freezer
Figure 1-2. IVF Controlled Rate Freezer (FDA clearance for human research)
Thermo Scientific
7007452 CryoMed Freezer
1-1
Section 1
Introduction
Standard Features
Figure 2-3. Control Panel Displays, Indicators and Keys
Door Latch
The door of the Controlled Rate Freezer is secured in the closed position
with a vise-action T-handle latch. The latch maintains positive
compression of the door gasket against the Teflon heatbreak to prevent
leakage and ice build-up during normal operation. The latch is closed by
turning the handle 180 degrees clockwise.
Note Make sure the door is properly latched during operation to prevent
ice build-up. ▲
Sample ‘Seeding’ Access
Ports (IVF)
Displays
1-2
7007452 CryoMed Freezer
The IVF model of the Controlled Rate Freezer has top-mounted sample
access ports used to ‘seed’ the samples during the freezing process. Seeding
should always be performed through the access ports rather than the door
to reduce sample temperature fluctuations during the seeding process.
There are three displays on the Controlled Rate Freezer control panel:
Chamber and Sample temperature, ranging from +54° to -199°C, the
LCD Display for profile status, messages, and freezing profile editing and
viewing.
Thermo Scientific
Section 1
Introduction
Indicators
At the top of the control panel are two columns of LED Operating Mode
indicators. The left indicators are:
Idle . . . . . . . . . . . . . . . . . . . . . .No profile is running
Run . . . . . . . . . . . . . . . . . . . . .A profile is in progress
Wait . . .Profile in progress pauses until the Run key is pressed
End . . . . . . . . . . . . . . . . . . . . . . . .Profile is complete
Warm . . . . . . . . . . . . .Chamber is warming to +25°C
The right column of indicators are:
Cool . . . . . . . . . . . . . . . .Single LN2 solenoid is open
Cool+ . . . . . . . . . . . . . .Both LN2 solenoids are open
Heat . . . . . . . . . . . . . . . . . . . .The heater is energized
Keys
There are seven input keys on the control panel.
Warm . . . . . . . . . . . . . . . . . .Starts the warming cycle
Run . . . . . . . . . . . . . . . . . . .Starts chosen profile, or resumes after Wait.
Silence . . . . . . . . . . . . . . . . .Turns off the audible alarm and clears the
alarm messages from the display
Back . . . . . . . . . . . . . . . . . .Terminates a profile in progress, returns to
Program menu, or moves the cursor to the left
Enter . . . . . . . . . . . . . . . . . .Starts a new line, enters a sub-menu,
moves the cursor to the right, or enters View or Edit Mode
▲ (Up) . . . . . . . . . . . . . . . . .Scrolls upward through display lines, or
changes profile step and set-up parameters
▼ (Down) . . . . . . . . . . . . . . .Scrolls downward through display lines, or
changes profile step and set-up parameters
Thermo Scientific
Alarm
Above the Silence key is the alarm indicator. In an alarm condition, the
LED flashes in addition to an audible tone. See Alarms, Section 2.
Output
Graphs and cycle data, as well as additional information, are printed out at
the top of the unit on a thermal printer in real-time mode. A connector is
also available on the back of the unit for an optional strip chart
temperature recorder.
7007452 CryoMed Freezer
1-3
Section 1
Introduction
Pre-set Profiles
The Controlled Rate Freezer has six pre-set freezing profiles. They are
labeled Pre-Set Profile 1 through 6. These profiles cannot be edited.
To view the profile timing and temperature:
1. Scroll, using the up or down arrow keys, to the desired profile.
2. Press the Enter key to go into View Mode. Scroll, using the up or
down keys, through steps as needed.
3. Press the Back key to return to the main menu.
The pre-set profiles are listed below. In addition, a Configure/Calibrate
menu is available. For further information on this menu, see Section 3.
User specific profiles can also be entered and saved. See Section 2.
Pre-Set Profile #1 - Commonly used for 2.0ml sample size, resulting in a
1°C rate from nucleation to -40°C and a 10°C per minute cooling rate to
a -90°C end temperature.
Step 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Wait
at 4.0C
Step 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m S
to -4.0C
Step 3 . . . . . . . . . . . . . . . . . . . . . . . . . . .25.0 C/m C
to -40C
Step 4 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C
to -12.0C
Step 5 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m C
to -40C
Step 6 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C
to -90C
Step 7 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .End
Pre-Set Profile #2 - Commonly used for very small (96 well) sample size,
resulting in a 1°C rate from nucleation to -40°C and a 10°C per minute
cooling rate to a -90°C end temperature.
Step 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Wait
at 4.0C
Step 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m S
to -40C
Step 3 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C
to -90C
Step 4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .End
1-4
7007452 CryoMed Freezer
Thermo Scientific
Section 1
Introduction
Pre-set Profiles
(continued)
Pre-Set Profile #3 - Commonly used for straw size samples, resulting in a
slow rate to -7°C, a 5 minute hold for manual cell nucleation, a 0.3°C
rate to -35°C, and then a 0.1°C per minute cooling rate to a -37°C end
temperature.
Step 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Wait
at 20C
Step 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m C
to -4.0C
Step 3 . . . . . . . . . . . . . . . . . . . . . . . . . . . .0.5 C/m C
to -7.0C
Step 4 . . . . . . . . . . . . . . . . . . . . . . . . . . .Hold for 5m
at -7.0C
Step 5 . . . . . . . . . . . . . . . . . . . . . . . . . . . .0.3 C/m C
to -35C
Step 6 . . . . . . . . . . . . . . . . . . . . . . . . . . . .0.1 C/m C
to -37C
Step 7 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .End
Pre-Set Profile #4 - Commonly used for hemopoietic stem cells that are
65-100ml in size, resulting in a 1°C rate from nucleation to -45°C and a
10°C per minute cooling rate to a -90°C end temperature.
Step 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Wait
at 20.0C
Step 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m S
to -6.0C
Step 3 . . . . . . . . . . . . . . . . . . . . . . . . . . .25.0 C/m C
to -50C
Step 4 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C
to -14.0C
Step 5 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m C
to -45C
Step 6 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C
to -90C
Step 7 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .End
Pre-Set Profile #5 - Commonly used for skin packets, resulting in a 1°C
rate from nucleation to -35°C and a 10°C per minute cooling rate to a
-90°C end temperature.
Step 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Wait
at 4.0C
Step 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m S
to -5.0C
Step 3 . . . . . . . . . . . . . . . . . . . . . . . . . . .20.0 C/m C
to -40C
Step 4 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C
to -10.0C
Step 5 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m C
to -35C
Step 6 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C
to -90C
Step 7 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .End
Thermo Scientific
7007452 CryoMed Freezer
1-5
Section 1
Introduction
Pre-set Profiles
(continued)
Pre-Set Profile #6 - Commonly used for hemopoietic stem cells that are
65-100ml in size, resulting in a 0.5°C rate from nucleation to -20°C and
a 1°C per minute cooling rate to a -80°C end temperature.
Step 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Wait
at 4.0C
Step 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m S
to -4.0C
Step 3 . . . . . . . . . . . . . . . . . . . . . . . . . . .20.0 C/m C
to -45C
Step 4 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C
to -10.0C
Step 5 . . . . . . . . . . . . . . . . . . . . . . . . . . . .0.5 C/m C
to -20C
Step 6 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m S
to -80C
Step 7 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .End
S = Sample Temperature
C = Chamber Temperature
C/m = degrees Centigrade per Minute
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Thermo Scientific
Section 2
Set-Up and Operation
Warning Nitrogen gas can cause suffocation without warning. Store and
use liquid nitrogen only in a well-ventilated place. As the liquid evaporates,
the resulting gas displaces the normal air in the area. (The cloudy vapor
that appears when liquid nitrogen is exposed to the air is condensed
moisture, not the gas itself. The issuing gas is invisible.) In closed areas,
excessive amounts of nitrogen gas reduces the concentration of oxygen and
can result in asphyxiation. Because nitrogen gas is colorless, odorless, and
tasteless, it cannot be detected by the human senses. Breathing an
atmosphere that contains less than 19.5% oxygen can cause dizziness and
quickly result in unconsciousness and death. Therefore, the use of oxygen
monitoring equipment is strongly recommended. ▲
Warning Contact of liquid nitrogen with the skin or eyes may cause
serious (freezing) injury. ▲
This freezer should be located in a well-ventilated area of the laboratory, on
a table/bench capable of supporting the unit’s weight (see Specifications
section), with adequate workspace available. Allow clearance for the open
door on the front of the unit, for the hose and power connections on the
back, and the exhaust vent on the left side. (Do NOT attach anything to,
or tamper with, the exhaust vent!) Locate the unit in close proximity to the
LN2 source.
Additional space for a
temperature recording device
may also be desired. The
connector for a recorder is
located on the back of the unit,
next to the power connection.
When closing the freezer door,
be sure to turn the handle 180°
in rotation to fully seal the
door.
Thermo Scientific
Figure 2-1. Connections on Unit Back
7007452 CryoMed Freezer
2-1
Section 2
Set-Up and Operation
Figure 2-2. Clearance for All Units
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Thermo Scientific
Section 2
Set-Up and Operation
Supplies
The Controlled Rate Freezer requires a clean, low pressure (22 PSIG,
±30%) LIQUID nitrogen supply. Do not use the gas fitting on the supply
tank! A dedicated supply tank/source is recommended for optimum
operation. The tank hose connection, 1/2” flare fitting, is located on the
back of the unit in the bottom left corner. Do not over-tighten when
connecting. Internal plumbing could be damaged. Teflon tape is NOT
recommended when installing LN2 supply. The tape cannot withstand the
low temperatures of this application. See Figure 2-1 for the connection
location.
Electrical
Connections
Refer to the electrical schematics or serial data tag on the unit for electrical
specifications for the Controlled Rate Freezer.
1. Verify the power switch on the back of the unit is off.
2. Attach the power cord to the inlet on the back of the freezer. Connect
the power cord to the appropriate power outlet.
Environmental
Conditions
The Controlled Rate Freezer is designed to be electrically safe in the following environmental conditions:
• Indoors
• Altitude: Up to 2,000 meters
• Temperature: 5°C to 40°C
• Humidity: 80% RH at or below 31°C, decreasing linearly
to 50% RH at 40°C
• Mains Supply Fluctuations: ±10% of nominal.
• Installation Category II 1
• Pollution Degree 2 2
• Class of Equipment I
1 Installation category (overvoltage category) defines the level of transient overvoltage
which the instrument is designed to withstand safely. It depends on the nature of the
electricity supply and its overvoltage protection means. For example, in CAT II which
is the category used for instruments in installations supplied from a supply comparable
to public mains such as hospital and research laboratories and most industrial
laboratories, the expected transient overvoltage is 2500V for a 230V supply and
1500V for a 120V supply.
2 Pollution Degree describes the amount of conductive pollution present in the
operating environment. Pollution Degree 2 assumes that normally only non-conductive
pollution such as dust occurs with the exception of occasional conductivity caused by
condensation.
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Section 2
Set-Up and Operation
Sample Thermocouple
Connection
Non-IVF models
1. Open the unit door.
2. Locate the thermocouple connector in the front upper right corner of
the chamber.
3. Install the supplied sample thermocouple connector in the correct
orientation.
4. Close the unit door.
IVF models
1. Locate the thermocouple connection on the top of the unit, to the left
of the printer cover (refer to Figure 1-1).
2. Install the appropriate supplied sample thermocouple in the correct
orientation.
Note The freezer has been calibrated with the internal thermocouple
connector. Refer to Section 3 for probe calibration. ▲
Power-Up
1. Turn the freezer power switch on the back of the unit to On.
2. On the control panel, the Alarm indicator lights briefly, and the LCD
display reads Controlled Rate Freezer XX (XX = software version).
3. The Idle indicator lights, then the LCD display shows the profile list.
4. To choose a profile, scroll through the selections using the up/down
arrow keys. The profiles are displayed in a loop. Pressing either of the
arrow keys consecutively brings the list back to the starting point.
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Thermo Scientific
Section 2
Set-Up and Operation
Access Code Security
The controller will provide security for the following menus and functions,
if the Access Code is enabled, by prompting for a 4-digit numeric access
code:
•
Configuration/Calibration Menu
•
Profile Edit Menu
•
Manual Override during a run
•
Start of a run on second or subsequent step in a profile
•
Manual change in Run profile execution to a prior or following step
•
Premature termination of a profile run
If access code protection is enabled (see the Configuration/Calibration
section), access to the above functions is gained by entering the access code
as follows:
1. Use the Enter and the Back key to select the desired digit. Use the up
or down arrow keys to change the digit. When finished, use Enter to
confirm entry of the code and to move into the selected protected
function or menu.
2. The access code when protection is disabled is 0 0 0 0.
3. The access code can be enabled, changed, or disabled by using
“Change Access Code” (see Configuration/Calibration section).
Initiate a Profile
1. Load the product into the freezing chamber. On IVF models, make
sure all unused sample access ports are capped.
2. If the profile list is not on the display, press Back until the list appears.
3. Locate the item selector (è) on the left of the top line of the menu.
4. Move the required profile to this line by using the 5/6 keys. The
chosen profile must be on the same line as the selector.
5. Press the Run key. If the chosen profile begins with a Wait, the Run
key must be pressed again to continue, after the appropriate
temperature is reached.
6. The mode indicator is lit, showing the status of the profile.
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2-5
Section 2
Set-Up and Operation
Initiate a Profile
(continued)
Note Any profile can be started at the second or any subsequent step by
entering View Mode (press Enter), scrolling (pressing the 5/6 keys) to the
desired profile step and pressing the Run key. If Access Code protection
has been enabled, the unit will prompt for the access code before entering
Run Mode at the selected step. ▲
Note The user can manually move to a different step in a running profile
by scrolling (5/6) to the desired profile step and pressing the Run key (the
move occurs when the Run key is released). If the user does not press Run
within 30 seconds, or Back to abort the move, the control will
automatically exit the the move mode and continue profile execution
without interruption. ▲
If Access Code protection has been enabled (see Configuration/
Calibration section), the unit will prompt for the access code before
moving to the selected profile step.
Stop a Profile Run
A profile run can be discontinued by pressing the Back key. If Access
Code protection has been enabled, the unit will prompt for the access code
before terminating the profile run. However, Access Code protection is not
required on an End step.
If a power outage interrupts a run, the profile will restart from the point of
interruption when power is restored. See Alarms in this section.
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Thermo Scientific
Section 2
Set-Up and Operation
Manual Override
The user may override a running freezing profile by manually injecting
LN2 or operating the heater to more rapidly change the chamber
temperature.
1. During a running profile, press the Enter key to access Manual
Override Mode. If Access Code protection has been enabled, the unit
will prompt for the access code before entering Manual Override
Mode.
2. Select the desired function to override (Heat, Cool or Cool+) by
pressing the Enter and Back keys.
Heat controls the heater.
Cool energizes the solenoid.
Cool+ energizes both solenoids.
3. Press and hold the up arrow key to turn On the selected function, or
press and hold the down arrow key to turn it Off. The function will
revert to normal profile control when the key is released.
Note The function not under manual control will still be under profile
control. For example, if the Cool function is activated under manual
override and the chamber drops below the programmed setpoint, the
controller will activate the Heat function to compensate. ▲
Program User
Specific Profiles
To program user specific profiles, enter Edit Mode. The following describes
the actions and options necessary for adding or editing a profile.
Note If Access Code protection has been enabled, the unit will prompt for
the code before entering Edit Mode. To enable an access code, see Access
Code Security in this section. ▲
If the profile list is not displayed, press the Back key as many times as is
necessary to view them.
1. Locate the arrow to the left of the option that is on the top line of the
display. Move the profile (USER 1 through USER 10) that you wish to
view or edit to the top line by pressing the 5or 6 keys.
Note Pre-set Profiles may not be edited. ▲
2. To enter View Mode, press the Enter key.
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Section 2
Set-Up and Operation
Program User Specific
Profiles (cont.)
3. Press the up arrow key to view Step 0. This is the Name/Title step.
4. Press Enter to go into Edit Mode for Step 0 and the cursor will be
blinking over the leftmost character position.
To change this character, scroll through the available characters by
pressing the up or down arrow keys. Press Enter when the chosen
character is visible. The cursor will blink over the next character in the
name or title. The available characters and their relative sequence is as
follows:
!“#$%&”()*+,_./
0 thru 9 : ; < = > ? @
A thru Z [ ] ^ _ ’ a thru z { | } → Sp
To delete a character, place the cursor over the chosen character and
press Silence.
Note When starting with a space ‘Sp’, pressing the up arrow key gives you
a ‘!’, while pressing the down arrow key gives you a ‘→’. ▲
5. At this time, enter a unique name or title for the profile.
6. When Step 0, the name or title, is complete, move the cursor back to
the leftmost position. This is View Mode.
7. Scroll, using the down arrow key, to Step 1.
8. Press Enter to go into Edit Mode.
Step 1 contains a command with a cursor blinking just to the right of a
left pointing arrow (← __ ).
With the cursor in this position, use the up or down arrow keys to
scroll through the available commands.
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Thermo Scientific
Section 2
Set-Up and Operation
Listed below are the available step types.
units
0.0 C/m C to 0.0C - Ramp chamber temperature at the specified rate
until the chamber reaches the specified temperature
0.0 C/m S to 0.0C - Ramp chamber temperature at the specified rate
until the sample reaches the specified temperature
For this particular function, when the cursor is in
the 'tens' position, pressing the up or down arrow
Ramp Function
keys changes the available function. The cursor
Number Position
must be in the 'units' position to change the rate
value. See Figure 2-3 for position location.
The monitored 'trip' temperature (either S for
0 0 . 0
Sample or C for chamber) can be changed by placFigure 2-3. Cursor
ing the cursor on the S or C and using the up or
Positions
down arrow keys.
tens
Program User Specific
Profiles (cont.)
Caution Steep ramp rates may cause chamber to be much colder/warmer
than the sample itself. ▲
Thermal controllers are limited to +55°C and -185°C during steep
Sample ramps.
Note For chamber ramp steps, the
interior set temperature will ramp
1°C beyond the programmed
temperature to assure that the ramp
end point condition will be reached
under all operating conditions. For
example, if the chamber set point is
30°C, the controller will ramp to
31°C until the setpoint is reached.
Graph print-outs show this ramp
Figure 2-4. Ramp Settings
setting. ▲
Thermo Scientific
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2-9
Section 2
Set-Up and Operation
Program User Specific
Profiles (cont.)
Wait at 0.0C: Go to specified chamber temperature and wait until the
Run key is pressed
Hold for 1m at 0.0C: Hold for specified time at noted chamber temperature, then continue. A double beep will sound at the end of the hold
time.
Precool: Cool chamber (w/ target of +4°C) and continue to next step when
a sample temperature of +5°C is reached
Jump to 1: Jump to a specified step
Loop to 1 for 1: Loop to step and repeat specified number of times
End: End of profile
9. After deciding on a function command, press Enter to move to the
temperature, time, or step number to be set. Scroll, using the 5/6 keys
to choose a number. Single clicks scroll slowly, or press and hold to
scroll more quickly. Press Enter to go to the next line in the profile.
10. After all function commands are chosen, press Back until the main
menu appears.
Note After exiting Edit Mode, the edited profile is printed on the thermal
printer, if printer is On and the selected output device. See Section 3. ▲
Note To edit a single line of a profile, enter View Mode, scroll to the
desired profile step and go to Edit Mode by pressing Enter. ▲
Copy and Paste Preset and User Profiles
Pre-set and created user profiles can be copied, then pasted into one of the
ten User Profiles as templates to create a new User Profile. To erase a User
Profile already in use, a blank User Profile may be copied, then pasted over
it. Use the steps below to copy and paste.
1. In the Main Menu, select the Pre-set or User Profile to be copied by
using the up or down arrow keys.
2. Press and hold (approximately 3 seconds) the Back key until the
message “Profile copied” appears on the second line of the display.
3. When the Back key is released, the message “Select dest with ”
appears on the third line of the display. Select the destination for the
copied profile, using the up or down arrow keys. When the desired
User Profile is showing on the first line of the display, press the Enter
key to complete the paste operation, or press Back to cancel.
2-10
7007452 CryoMed Freezer
Thermo Scientific
Section 2
Set-Up and Operation
Recorder
The recorder output connector on the back of the Controlled Rate Freezer
provides an analog signal representing process temperature(s) that can be
plotted on an optional strip chart recorder. The controller can be set to
output Chamber or Sample temperature, a scanned combination of both,
or Off. Refer to Section 3.
Note The chart recorder function is calibrated to match the non-linear
scale on the chart recorder paper (P/N 197044). ▲
Printer
The printer on the top of the Controlled Rate Freezer provides a visual
record of a profile run, including any errors which might have occurred.
Run data is captured in a printer buffer for later reprinting, even if the
printer is not enabled during the Run. The printer buffer is cleared at the
start of each new run.
It is recommended that a supply of printer paper be kept on hand. If paper
should run out during a freezing run, first install the paper. Then when the
Run is complete, the print-out may be re-started.
Typical profile listing after an edit
Run start time and date
Profile name
1234
cell culture media
D.M.S.O.
User information
2mL
12
Time/Temp scale
Sample temp
Chamber temp
Profile steps listed as they occur
Temp tracking alarm
Profile line
Figure 2-4. Print-outs
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2-11
Section 2
Set-Up and Operation
Alarms
Chamber Thermocouple
Open
•
Alarm messages are shown on the fourth line of the LCD display
•
Alarm messages alternate if multiple alarms are present at one time
•
Alarms are cleared with the Silence key
•
When an alarm message is shown, the unit will alternate between the
alarm message(s) and the normal fourth line display at a rate of 2
seconds per message.
•
Silence key - First depression silences the audible alarm. The second
depression turns off the Alarm light and clears the message on the
display if the alarm condition is resolved.
If a chamber thermocouple cannot be detected:
•
Audible alarm sounds continuously,
•
Alarm indicator lights,
•
Message “C sensor fail” shows on the LCD display,
•
“ILL” shows on the numeric display (chamber),
•
Freezer switches to Idle Mode and operating functions are disabled.
The audible alarm is silenced by the first depression of the Silence key.
When the alarm condition has cleared, another depression of the Silence
key is necessary to turn off the Alarm LED and clear the alarm message
from the fourth line of the display. The numeric display message will
revert to a normal temperature display when the alarm condition clears.
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Thermo Scientific
Section 2
Set-Up and Operation
Sample Thermocouple
Open
The freezer responds to an open Sample thermocouple in different ways,
depending on what the unit is doing at the time or which freezing profile
has been selected.
1. Idle Mode - If the probe fails or is removed by the operator during
sample loading, while unit is in Idle Mode:
•
Alarm LED lights (no audible alarm),
•
LCD Display shows “S sensor fail” on the fourth line,
•
Sample numeric display shows “ILL”.
The operator may start a run that does not reference the Sample
temperature, if the Sample probe is removed or open, and the Alarm LED
and the fourth line LCD message are cleared using the Silence key. The
run will proceed normally.
If the operator attempts to start a run using the Sample temperature for a
trip point,
•
Audible alarm sounds,
•
Run start prevented,
•
LCD Display shows “S sensor fail” on the fourth line, alternated with
“S sensor req.”
The Alarm LED and display messages remain active until the probe
condition is resolved.
2. Run Mode - If the Sample probe fails during Run Mode, and the
current profile uses the Sample temperature as a trip point:
•
Alarm LED lights,
•
Audible alarm sounds continuously,
•
LCD Display shows “S sensor fail” on the fourth line, alternated
with “S sensor req.”,
•
Sample numeric display shows “ILL”,
•
Immediately switches to Idle Mode,
•
Disables all control functions.
Pressing the Silence key will mute the audible alarm, however, the Alarm
LED and display message will remain active until the probe condition is
resolved.
Thermo Scientific
7007452 CryoMed Freezer
2-13
Section 2
Set-Up and Operation
Sample Thermocouple
Open (continued)
If the Sample probe fails during Run Mode and the current profile does
not use the Sample temperature as a trip point:
•
Alarm LED lights,
•
Audible alarm sounds continuously,
•
LCD Display shows “S sensor fail” on the fourth line,
•
Sample numeric display shows “ILL”,
•
Continue run normally.
Depressing the Silence key will silence the audible alarm and clear the
Alarm LED and display messages. The numeric display “ILL”, will remain
until the probe condition is resolved.
Heater Malfunction
If the heater is energized continuously (no active temperature control) for
60 minutes, the controller will sound a heater malfunction alarm, light the
Alarm LED, sound the audible alarm continuously, show the message
“Heater error” on the fourth line of the display and immediately switch to
Idle Mode and disable all functions.
The audible alarm is silenced by pressing the Silence key. Since this alarm
indicates a hard failure, the Alarm LED and fourth line message can only
be cleared by turning the unit power off, then on again.
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Thermo Scientific
Section 2
Set-Up and Operation
Chamber/Sample
Over/Under Limit Temp
Caution Do not allow the sample probe to come into contact with the
chamber interior during operation. This could cause a displayed Sample
probe temperature shift. ▲
If the temperature of the Chamber or Sample probe reaches the chamber
design high or low temperature limit (design temperature limits are factory
pre-set at +55°C and -185°C), the controller will:
1. Sound a Temperature Limit alarm,
2. Light the Alarm LED,
3. Sound the audible alarm at a 75% on-duty cycle (2 second period),
4. Show “C temp limit” or “S temp limit” on the fourth line of the
display depending on which probe is at (or exceeding) the design
temperature limit (alternated with normal fourth line display contents
at a 2 second rate),
5. Continue the profile run without interruption.
This is a “self clearing” alarm. While in the alarm condition, the audible
alarm can be silenced by one depression of the Silence key, but the Alarm
LED continues to flash and the alarm message displays as long as the
alarm condition is present. When the chamber temperature returns to a
value within the pre-set limits, the Alarm LED stops flashing and the
audible alarm mutes (if not already muted by pressing the Silence key).
However, the alarm message remains on the fourth display line until the
Silence key is depressed. If the temperature of either probe continuously
exceeds the pre-set limit temperatures for more than 10 minutes, the unit
will immediately switch to Idle Mode and disable all functions.
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2-15
Section 2
Set-Up and Operation
Temperature Tracking
Alarm
During a Run when the chamber temperature is higher or lower than the
programmed temperature by a pre-determined amount for a predetermined time period (as set in Section 3), the controller will:
•
Sound a tracking alarm,
•
Light the Alarm LED,
•
Sound the audible alarm at a 75% On duty cycle (2 second period),
•
Show “Temperature is High” or “Temperature is Low” depending on
conditions, (compared to the programmed temperature) on the fourth
line of the display, alternated with the normal fourth line display
contents at a 2 second rate,
•
Mark the strip chart recorder and thermal printer at 50°C to document
on paper where the alarm occurred,
•
Continue the profile run without interruption.
This is a “self clearing” alarm. When in the alarm condition, the audible
alarm can be silenced by one depression of the Silence key but the Alarm
LED will continue to flash and the alarm message be displayed while the
alarm condition is present. When the alarm condition is resolved, the
Alarm LED will stop flashing and the audible alarm will silence (if the
Silence key has not already been pressed). However, the alarm message will
remain on the fourth display line until the Silence key is pressed a second
time.
2-16
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Thermo Scientific
Section 2
Set-Up and Operation
Power Failure
The controller will detect a power failure and alert the operator under the
following conditions:
1. Power failure less than 10 seconds in duration
Continue profile run without interruption or alarm after power is
restored.
2. Power failure more than 10 seconds in duration
Flash Alarm LED and sound audible alarm at 25% On duty cycle with
2 second period
Show duration of failure and “Power Failure Alarm” on second, third
and fourth line display) display line (alternated with normal fourth line
display)
Mark strip chart recorder and thermal printer (re-print temperature
scale on thermal printer)
Continue profile run without interruption.
A single depression of the Silence key will clear the audible alarm. A
second depression of the Silence key will clear the Alarm LED and the
messages on the second, third and fourth display lines.
Run Complete
Five minutes after the End profile step is reached, the controller will sound
the Run Complete alarm:
•
Flash Alarm LED and audible tone at a 25% On duty cycle (2 second
period)
•
Show “End of run” on the fourth line of the display.
The controller will maintain the temperature of the final step in the profile
run until the run is stopped by the operator pressing the Back key.
Caution If performing successive runs, dry the gaskets and heat breaks
between each run. ▲
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Section 2
Set-Up and Operation
Warm Complete
Five minutes after the chamber reaches 25°C during a Warm cycle, the
controller will sound a Warm Complete alarm:
•
Flash the Alarm LED and sound an audible tone at a 25% On duty
cycle (2 second period);
•
Show “Warm Complete” on the fourth line of the display.
Chamber temperature is maintained at 25°C for a period of 30 minutes,
then switched to Idle Mode and all functions are disabled.
Seeding Hold
When the ‘Hold’ time period has elapsed, two audible tones sound to
notify the user of the completion of the ‘Hold’ step and the continuation
of the profile run. This can be used to alert the user that a particular point
has been reached in the profile.
When seeding, be sure to use the top access ports rather than the door.
Printer Buffer Full
This alarm message appears when the printer storage buffer is full of Run
data (approximately 2 hours) and is no longer able to store data for later
printing. Press Silence to mute the alarm. However, the LED will continue
to flash until the Run is stopped. Press Silence to clear the alarm message.
Between the time the ‘Printer Buffer Full’ messsage displays and the user
stops the Run, no data is captured. The printer buffer will store the
previously captured data until the next Run is started. The buffer is
cleared at the start of each new Run.
Note: The printer continues to print current Run data even though the
‘Printer Buffer Full’ alarm has occurred.
IVF Sample Holders
2-18
7007452 CryoMed Freezer
Included with the IVF Controlled Rate Freezer are a straw holder, vial
holder, cane holder and access port plugs.
Thermo Scientific
Section 2
Set-Up and Operation
Using a Vial Holder
The vial sample holder secures a vial for insertion through the access port
on the top of the IVF freezer. This holder may also be used to hold the
external sample probe. See Figure 2-5.
1. To secure the vial, first make sure the
holder is in an upright (vertical)
position.
2. Press the button on the top of the
holder. This temporarily widens the
opening at the bottom of the holder to
install the vial.
3. Carefully let the button up on the top
of the holder while making sure the
vial fits into the indentation of the
holder. The vial should be secure in
the holder when the button is released.
4. If the holder is securing the sample
probe vial (hole in vial cap), slide the
probe down through the holder until
Figure 2-5. Vial Holder
it feeds through the vial cap.
5. Install the other end of the probe to the external connection on the top
of the unit. See Figure 1-2.
Using the Straw Holder
The straw sample holder secures up to 4 straws for insertion through the
access port on the top of the IVF freezer. The straw holder may also be
used to hold the external sample probe. See Figure 2-6.
1. To secure the straws, first make sure the holder is in an upright
(vertical) position.
2. Press the button on the top of the holder. This temporarily widens the
opening at the bottom of the holder to install the straws.
3. Carefully let the button up on the top of the holder while making sure
each straw fits into an indentation of the holder. The straws should be
secure in the holder when the button is released.
4. To install the straw probe, insert the probe down the center of the
button indicated below. Slide the probe down through the holder until
it feeds through the center straw.
Thermo Scientific
7007452 CryoMed Freezer
2-19
Section 2
Set-Up and Operation
Using the Straw Holder
(continued)
5. Install the other end of the probe to the external connection on the top
of the unit. See Figure 1-2.
Using the Cane Holder
Figure 2-6. Straw Holder
Figure 2-7. Cane Holder
The cane sample holder secures a cane for insertion through the access
port on the top of the IVF freezer. See Figure 2-7. To use the cane holder,
first assemble the aluminum holder to the plastic handle. Insert the cane
holder assembly through the access port on the top of the unit. See Figure
1-2.
If the cane holder is used with a sample probe, use the standard 1.2ml vial
probe. Connect the probe to the internal sample probe connection.
Caution Do not connect two sample probes at the same time. ▲
2-20
7007452 CryoMed Freezer
Thermo Scientific
Section 2
Set-Up and Operation
Holder Adjustment
The vial and straw holders may be adjusted to the size needed. Refer to the
illustrations following.
1. Loosen the small hex socket set screws in the top of each holder.
2. Rotate each of the long adjustment rods equally, to the right to make
them longer (widen the opening), or to the left to make them shorter
(narrow the opening). Note the threads at the bottom of the
adjustment rods.
3. Tighten the small hex socket set screws when the adjustment is
complete, just until the screws lightly touch the rods to avoid
damaging the rod threads.
Figure 2-7. Vial Holder Adjustment
Figure 2-8. Straw Holder
Thermo Scientific
7007452 CryoMed Freezer
2-21
Section 3
Configuration/Calibration
The Configuration/Calibration Menu is selected by pressing the Enter key
with the Configure-Calibrate line selected. The software will prompt for
the Access Code, if enabled, prior to entering the Configuration/
Calibration Menu.
Menu Structure
Menu Entry
Definitions
The following is the menu structure.
Configure - Calibrate
Change Access Code
Chart Recorder
Printer
Probe Calibration
Service
Manual Control
Print Error Logs
Reset Error Logs
Simulation
Software Checksum
Test Ram
Thermal Controller
Use Factory Defaults
Time and Date
Tracking Alarms
1. Configure-Calibrate
A. Change Access Code:
Change the four digit security access code by selecting each digit
using the Enter and Back keys, and changing the digit using the
up/down arrow keys. The default Access Code (security disabled) is
0 0 0 0. Each digit may be set over a range of 0 to 9. Exit this
menu by using the Back key.
Thermo Scientific
7007452 CryoMed Freezer
3-1
Section 3
Configuration/Calibration
Menu Entry
Definitions (cont.)
B. Chart Recorder:
Selects the chart recorder operating parameters and checks
calibration. Exit this menu by using the Back key.
a. Select Chart Recorder Output:
Select chamber only, sample only, chamber/sample scan (2 sec.
chamber, 10 sec. sample), or no chart recorder function (Off ).
The default setting is Off.
b. Calibration/Linearity Verification:
Verify chart recorder calibration and linearity by setting the chart
recorder function over a range of -180°C (0VDC) to +50°C
(approximately 10VDC) in 10° steps.
Note The chart recorder function matches the non-linear scale on the chart
paper (P/N 197044). ▲
Optional Flatbed Recorder Calibration Procedure
1. Press the power switch on the flatbed recorder and allow the system to
warm-up for 20 minutes.
2. Uncap and lower the pen to the graph paper.
3. From the freezer Configure-calibrate menu, select Chart Recorder. Turn
the chart recorder on to Scan mode and press the Enter key.
4. Set the temperature to 0°C (zero). IMPORTANT: If the temperature is
already set to 0°C, move it up or down 10°C, then back to 0°C. The
recorder pen should move to 0°C. If it does not, adjust it using the zero
knob on the recorder until the pen reaches 0°C.
5. Set the temperature to -180°C to simulate the low end of the scale. The
recorder pen should move to -180°C. If it is out of calibration, adjust it
using the small screw (accessible through the hole adjacent to the zero
knob) until the pen reaches -180°C.
6. Recheck zero calibration as directed in Step 4. Repeat Steps 4 and 5 if
necessary.
C. Printer Menu:
Selects the thermal printer operating mode, printer scale, or
initiates printing of the previous run chart. Select the desired item
using the Enter and Back keys and change the selected parameter
using the 5/6 keys. Exit the menu using the Back key.
3-2
7007452 CryoMed Freezer
Thermo Scientific
Section 3
Configuration/Calibration
Menu Entry
Definitions (cont.)
a. Select Printer Output:
Select Chamber Only, Sample Only, Chamber/Sample Scan, or No
Printer (Off ). The default is Scan.
b. Enable/Disable Profile Set Temperature Line:
Turn On or Off a line on the thermal print-out that shows the set
temperatures and ramps as the freezing profile is run and printed.
The default is Off.
c. Previous Run:
A duplicate of the print-out made during the Run. The print-out
reflects the actual time and date of the run, rather than the current
time and date.
Note The unit will store at least 2 hours of data. ▲
d. Select Printer Temperature Scale:
Select one of two printer scales; -180C to +50C (default) or -100C
to +50C.
e. Select Printer Time Scale:
Select 1, 2, or 5 minutes per division.
2. Probe Calibration:
Allows operator to calibrate the Chamber or Sample probes by entering
an offset and scale factor (scaler). Exit this menu by using the Back
key. Use an appropriate reference thermometer for comparison near
0°C (offset) and -196°C (scaler).
Note The chamber/sample limit temperature alarm will sound during the
scale factor adjustment at -196°C. Silence the alarm during the calibration
procedure by pressing the Silence key. Clear the alarm message after the
procedure is complete by pressing the Silence key one more time. ▲
Important After calibrating the temperature probe,
reinstall the probe in the previous position. Make
sure the tape on the probe wire is flush with the
frame for consistent probe results. ▲
Figure 3-1. Install Probe
1. Probe Offset:
The Chamber and Sample probe offset can be adjusted over a
range of -9.9°C to +9.9°C in 0.1°C increments. Adjust the probe
offset for a 0.0°C temperature display reading with the probe in a
standard crushed ice bath.
Thermo Scientific
7007452 CryoMed Freezer
3-3
Section 3
Configuration/Calibration
Menu Entry
Definitions (cont.)
2. Probe Scaler:
The Chamber and Sample probe scale factor (scaler) can be
adjusted over a range of 0.5000 to 1.9995 in 0.0005 increments.
Adjust the probe scaler for a -196°C temperature display reading
with the probe in liquid nitrogen.
Warning Contact of liquid nitrogen with the skin or eyes may cause
serious freezing (frostbite) injury. ▲
Thermocouple Probe Calibration Procedure:
Note A container of “pooled” liquid nitrogen and a container of crushed
ice and water (crushed ice just covered with water) are required for this
procedure. Prepare both containers before starting the procedure. ▲
1. Turn the unit on and enter the Configuration/Calibration menu. Allow
the unit to warm-up for 10 minutes.
2. Remove the end of the chamber thermocouple probe from the
mounting tube (near sample thermocouple connector inside the
freezing chamber).
3. Dip the Chamber and Sample probes in the water/ crushed ice
container and allow to stabilize for 5 minutes. The probes must be in
the center of the container, well away from the container walls.
4. Observe the Chamber and Sample temperature displays. Adjust the
Chamber and Sample ‘Offset’ parameters so that the displays each read
0.0°C. Move the cursor to the proper parameter by using the Enter/
Back keys and change the parameter using the up/down arrow keys.
Each degree or tenth of a degree of Offset will result in a respective
change in the corresponding temperature display.
5. Dip the Chamber and Sample probes in the liquid nitrogen container
and allow to stabilize for 5 minutes. The probes must be in the center
of the container, well away from the container walls.
6. Observe the Chamber and Sample temperature displays. Adjust the
Chamber and Sample ‘Scaler’ parameters so that the displays each read
-196°C. If several scaler values result in the desired reading, select a
scaler value in the middle of the range.
7. Repeat Steps 3 through 6 as necessary until both temperature displays
read 0.0°C and -196°C. There may be some interaction between the
‘Offset’ and the ‘Scaler’ adjustments.
3-4
7007452 CryoMed Freezer
Thermo Scientific
Section 3
Configuration/Calibration
Menu Entry
Definitions (cont.)
3. Service
This menu is for use by qualified service personnel. Entry into this
menu has a required service access code.
Warning This area may be accessed by qualified service personnel only. ▲
A. Manual Control
Individually or in any combination, turns on fan, heater, Cool
solenoid and Cool+ solenoid for diagnostic or troubleshooting
purposes and displays the status of each component. Upon
entering the menu, the Enter and Back keys position a cursor over
the item to control. The up arrow turns On the selected item and
the down arrow turns it Off. When the menu is exited (Back key),
any function that has been turned On will be turned Off and the
unit reverts to Idle Mode.
Note Fan will run whenever the heater is energized. The solenoid closest to
the front of the unit will be energized when Cool is activated. ▲
B. Print Error Logs
This menu item prints and resets the diagnostic error logs that are
stored in battery RAM. Each error log consists of the alarm
message and the time/date of occurrence. A maximum of 20 alarms
for each alarm type are stored in the log. Older entries are
discarded when more than 20 alarms are accumulated.
The following is a list of alarm types stored.
•
Chamber/Sample probe failure during a run
•
Chamber/Sample over/under temperature limit
•
Power failure (also stores power failure duration)
•
Heater error
•
Check LN2 supply
•
RS-232 communication error
C. Reset Error Logs
This menu item is used to clear the Error Log entries. When the
log is empty, this item reads “Error Log Empty”.
Thermo Scientific
7007452 CryoMed Freezer
3-5
Section 3
Configuration/Calibration
Menu Entry
Definitions (cont.)
D. Simulation:
This menu item enables a thermal simulator that allows the unit to
run freezing profiles in a realistic manner without actually using the
chamber heater or cooling solenoids. The simulator uses separate
parameters for heating and cooling effects that approximate the
performance of the real chamber.
The delays supported are:
•
Turn On delay (Del)
•
Turn Off delay (Eff )
•
Rate of temperature change per minute (C/M)
Simulation mode is enabled by changing the “sim” parameter from
0 to 1, exiting the Simulation menu and cycling the AC power
Off/On. When the unit powers back up, “sim” will be appended to
the software version number on the initial power-on display of the
4 X 20 LCD. The simulation mode is disabled by changing the
“sim” parameter from 1 to 0, exiting the Simulation menu and
cycling the AC power Off/On. “sim” will no longer be appended to
the software version number on the initial power-on display.
E. Software Checksum
This menu item initiates a checksum calculation of the software in
controller memory and compares it to the reference value loaded in
the controller when the software was installed. In addition, the
checksum value is shown in the lower right corner of the display.
F. Test RAM
This menu item initiates a non-destructive test of all RAM on the
controller board (S-RAM, BAT-RAM). Any errors found would be
reported on the LCD display (i.e., “Memory failure, check UXX”).
G. Thermal Controller
Adjust the cycle period of the thermal controller and integral
proportional, differential gain of the P.I.D. temperature controller,
the minimum solenoid on time, the temperature difference from
the setpoint that the Cool+ solenoid is actuated, and the difference
in P.I.D. parameters for the heater vs. LN2 injection cooling. Exit
this menu using the Back key.
There is a separate set of parameters for each size of chamber
available ([L] 1.7 cu ft, [M] 1.2 cu ft, and [S] 0.6 cu ft).
3-6
7007452 CryoMed Freezer
Thermo Scientific
Section 3
Configuration/Calibration
Menu Entry
Definitions (cont.)
The following is a listing of parameters with their defaults.
•
Size: L, M, S (default is M)
•
Cycle Time adjusted over a range of 500 to 2000mS (default is
1000mS)
•
P: Proportional gain adjusted over a range of 0.0000 to 0.9999
(default 0.25)
•
I: Integral gain adjusted over a range of 0.000 to 0.999 (default
0.075)
•
D: Differential gain adjusted over a range of 0.00 to 9.99 (default
0.20)
•
D/C: Discontinuous or Continuous operation of the controller
when the probe temperature is the same as the set temperature
(default C)
•
PR: Cool/Cool+ solenoid precision (minimum on time) adjusted
over a range of 0.01 to 0.99 (default 0.01 or 50mS minimum on
time)
•
CP: Cool+ differential temperature adjusted over a range of 1°C to
99°C (default 4°C)
•
CM: Cool multiplier determines the cooling proportional and
differential gain constants relative to the constants entered for P
and D, i.e., P (cool) = P*CM, with a range of CM from 0.1 to 9.9
(default 1.0).
H. Use Factory Defaults
This menu item restores the factory-set parameters for the
following items.
•
Access Code (0 0 0 0, disabled)
•
Probe Calibration (offset and scaler 0)
•
Simulation (Off; Cool: 1S, 10S, 90C/m; Heat: 1S, 45S,
9C/m)
•
Thermal Controller (Size M, 1000mS, P: 0.25,
I: 0.075, D: 0.2, PR: 0.01, CP: 4, CM: 1.0, C)
•
Tracking Alarms (5C, 1m)
Note User Profiles are not affected. ▲
Thermo Scientific
7007452 CryoMed Freezer
3-7
Section 3
Configuration/Calibration
Menu Entry
Definitions (cont.)
D. Time and Date:
Select the parameter to change, using the Back and Enter keys, and
change the selected parameter, using the up/down keys. Exit this
menu using the Back key.
The following parameters are set.
• Day of the week (Sun, Mon, Tue, Wed, Thu, Fri, Sat)
• Month (Jan, Feb, Mar, Apr, May, Jun, Jul, Aug, Sep, Oct, Nov,
Dec)
• Date (01-31)
• Year (2000 - 2098)
• Hour (12 hour format, 1 - 12)
• Minute (00 - 59)
• Second (00 - 59)
• AM/PM
E. Tracking Alarms:
Adjust the High and Low temperature tracking alarms for duration
of run (Time - 1 minute increments) and temperature difference
from setpoint (Delta - 1°C increments). The Time parameter can
be adjusted over a range from a 1 to 99 minute range. Exit the
menu using the Back key.
3-8
7007452 CryoMed Freezer
Thermo Scientific
Section 4
Maintenance
Warning Be sure to disconnect the unit from the power source before
beginning any service work. ▲
Caution Before using any cleaning or decontamination method except
those recommended by the manufacturer, users must check with the
manufacturer that the proposed method will not damage the equipment. ▲
Caution Do not use strong alkaline or caustic agents. Stainless steel is
corrosion-resistant, not corrosion-proof. Do not use a solution of sodium
hypochlorite (bleach) as it can cause pitting and rusting. ▲
Warning Alcohol, even a 70% solution, is volatile and flammable. Use it
only in a well ventilated area that is free from open flame. If any
component is cleaned with alcohol, do not expose the component to open
flame or other possible hazard. Allow the alcohol to fully dry before
turning power on. ▲
Disinfect Controlled
Rate Freezer Interior
Use an appropriate disinfectant. All articles and surfaces to be disinfected
must be thoroughly cleaned, rinsed and roughdried.
1. Turn the freezer off and disconnect the plug from the power source.
2. Unplug and remove the Sample probe from the chamber, clean with
alcohol and allow to dry.
3. Wash the cabinet interior with disinfectant starting at the top and
working down. The cabinet and door must be rinsed with sterile water
until the disinfectant has been removed. After the cabinet has been
rinsed, spray with 70% alcohol.
4. Allow the cabinet door to remain open for the alcohol to evaporate.
5. Plug the freezer in and turn the power switch on.
Thermo Scientific
7007452 CryoMed Freezer
4-1
Section 4
Maintenance
Clean a Major Spill in the
Chamber
1. Turn the unit off and disconnect it from the power source.
Warning Be sure to disconnect the power source as the interior fan may
start unexpectedly, under certain conditions. ▲
2. Disconnect the probe from the connector, if applicable. Remove the
sample holders (IVF only).
3. Remove the two screws at the top and bottom of each side, securing
the screened side ducts to the chamber. Set the ducts aside.
4. Press the top and bottom edges of the center duct toward each other
and draw the duct outward to remove.
5. The chamber is now fully accessible and can be cleaned.
6. Clean the interior of the unit with an appropriate disinfectant. Clean,
rinse and wipe dry, as needed. Clean the screened side ducts and center
ducts before reinstalling.
7. Press the top and bottom edges of the center duct toward each other
and insert the duct into the center of the chamber. The edges fit inside
the door gasket.
8. Install each screened side duct. Locate the tabs on the back of each
duct into the proper holes at the back of the chamber. Align the two
screw holes and secure at the top and bottom of each side.
Caution Be sure all chamber components are re-installed in the proper
locations to avoid compromising the chamber airflow and temperature
uniformity. ▲
Caution Avoid damaging the chamber opening gasket. ▲
9. Connect the Sample probe to the connector, if applicable.
10. All chamber parts must be re-installed for consistent uniformity and
airflow. Connect the unit to the power source and turn it on.
11. The freezer is ready to be returned to service.
4-2
7007452 CryoMed Freezer
Thermo Scientific
Section 4
Maintenance
Clean the Cabinet
Exterior
Clean the freezer exterior with a damp sponge or soft, well-wrung cloth
and mild detergent dissolved in water. Dry with a soft cloth.
Note On IVF models, be sure to clean between the sample access openings
on the top of the unit. ▲
Clean Gasket/Heat
Break
Thermo Scientific
Dry any moisture from the door gasket and heat breaks between each run,
using a soft cloth, to prevent ice build-up which could reduce the
effectiveness of the door seal.
7007452 CryoMed Freezer
4-3
Section 5
Printer Set-Up
The built-in thermal printer has internal software switch settings that
control how the printer interfaces with the Controlled Rate Freezer. These
internal settings are pre-set at the factory for proper operation with the
freezer. However, if the printer has to be replaced in the field, the settings
must be changed to interface correctly with the freezer. The following chart
shows the parameters which may be set, the printer manufacturer default
settings and the settings required by the Controlled Rate Freezer. See the
included printer manual for the software switch setting procedure.
Change the Paper
1. Turn the unit on.
2. Trim the paper as needed to make edge straight.
paper
3. Open the printer cover.
4. Feed the paper into the inlet at the bottom of the paper holder until
the auto-loader begins to pull the paper in. The auto-loader will feed
about 4 inches (10cm) through the paper cutter.
5. Press the paper feed switch until the paper
feeds in straight and smoothly.
Insertion
Slot
The printing surface is the outside of
the thermal paper roll.
Thermo Scientific
7007452 CryoMed Freezer
5-1
Section 5
Printer Set-Up
Table 5-1. Internal Settings
5-2
7007452 CryoMed Freezer
Parameter
Default from manufacturer Required for CRF
DIP SW-1
(switch setting)
(switch setting)
1: Input
Parallel (ON)
Serial (OFF)
2: Printing Speed
High (ON)
High (ON)
3: Auto paper loading
Enabled (ON)
Enabled (ON)
4: Auto line feed
Disabled (OFF)
Disabled (OFF)
5: DIP switch setting
Enabled (ON)
Enabled (ON)
6: Print density (a)
100% (OFF)
100% (OFF)
7: Print density (b)
(ON)
(ON)
8: Print density (c)
(ON)
(ON)
DIP SW-2
(switch setting)
(switch setting)
1: Print mode
40 columns (ON)
40 columns (ON)
2: User font back-up
Enabled (ON)
Enabled (ON)
3: Character type
Normal (ON)
Normal (ON)
4: Zero style
Normal (ON)
Normal (ON)
5: Character set (a)
Japanese (ON)
USA (ON)
6: Character set (b)
(ON)
(ON)
7: Character set (c)
(ON)
(ON)
8: Character set (d)
(ON)
(OFF)
DIP SW-3
(switch setting)
(switch setting)
1: Data length
8 Bits (ON)
8 Bits (ON)
2: Parity (with/without)
Without (ON)
Without (ON)
3: Parity (odd/even)
Odd (ON)
Odd (ON)
4: Busy control
Hardware (ON)
XON/XOFF (OFF)
5: Baud rate (a)
75bps (ON)
9600bps (OFF)
6: Baud rate (b)
(ON)
(ON)
7: Baud rate (c)
(ON)
(ON)
8: Baud rate (d)
(ON)
(ON)
Thermo Scientific
Section 6
Specifications
Temperature Uniformity
Temperature uniformity is specified to be less than a standard deviation
of 2°C (up to the ‘freeze’ of the first vial) across a matrix of 10 vials
(1.2/2.0ml size) containing 1.0ml (± 2%) each of distilled H20. The
temperature is to be monitored by type ‘T’ thermocouples placed in the
center of the liquid in each vial with the vials distributed in standard
racks and holders as shown below (separately for each cabinet size).
Please note that there are 5 each vials in the second rack from the top, as
well as the second rack up from the bottom, with the ‘Sample’ vial
placed in the center of the top rack. Also, note that the large cabinet
uses a combination of the small and mid-size rack and holder sets.
Finally, a specific program sequence, listed below, must be run in order
to collect the uniformity data.
Rack and Holder Vial Placement for each size chamber
Thermo Scientific
0.6 cu. ft.
1.2 cu. ft.
1.7 cu. ft.
Models
7450/7451
7456/7457
7472, 7475
Models
7452/7453
7458/7459
7473, 7476
Models
7454/7455
7474
7007452 CryoMed Freezer
6-1
Section 6
Specifications
Uniformity Profile
1) Hold 10.0min @25C
Weight
Model
lbs. (kg)
2) 2.0C/min C to 0.0C
7450
140 (63.5 )
3) 0.0C/min S to 3.0C
7451
140 (63.5)
4) Hold 10.0min @1.0C
7452
160 (72.6)
5) 1.0C/min C to -5.0C
7453
160 (72.6)
6) 99C/min C to -50C
7454
180 (81.6)
7455
180 (81.6)
7456
140 (63.5)
7457
140 (63.5)
9) Hold 12.0 min @ -2.0C
7458
160 (72.6)
10) 1.0C/min C to -20C
7459
160 (72.6)
11) END
7472
140 (63.5)
7473
160 (72.6)
7474
180 (81.6)
7475
140 (63.5)
7476
160 (72.6)
7) 99C/min C to -10.0C
8) 1.0C/min C to -2.0C
Chamber Temperature Range
+50°C to -180°C
Chamber Freezing Rate
Minimum 0.1°C / min
Maximum 99.9°C / min over operating temperature range
Note Rates vary based on load, LN2 pressure, unit size, and voltage. ▲
Chamber Warming Rate
Minimum of 0.1°C / min (no load, T-couple in free air, center of
chamber)
Maximum of 12.0°C / min (same conditions as above)
Average of 9.0°C / min (same conditions as above, range -40°C to -12°C)
Note Rates vary based on load, LN2 pressure, unit size, and voltage. ▲
6-2
7007452 CryoMed Freezer
Thermo Scientific
Section 6
Specifications
LN2 Injection
Patented liquid refrigerant injection ring optimizes uniformity and air flow.
Patent # 6,044,648
Operating Environment
Ambient Temperature: 15°C to 35°C
Relative Humidity: 20% to 85% non-condensing
Insulation
Foamed-in-place polyurethane
Air Flow
Horizontal from right to left, as viewed from the front
Sound Level
Unit in operation does not exceed 65 dBA.
Operational Features
Door interlock switch turns off LN2, heater and fan motor, except during
the warm-up cycle.
During the warm-up cycle, the door can be opened with heater and fan
running (up to 0°C) to accelerate warming.
LN2 pressure relief valve located adjacent to LN2 connection.
Chamber pressure vent port located on left side-wall.
Heater overtemperature protection: At a temperature level of 93°C, a
manual reset device interrupts power to the heating element.
External Connections
Power entry
Strip chart temperature recorder
PC interface port
LN2 connection 1/2” flare
External thermocouple jack (IVF models only)
Sample access (IVF models only)
Thermo Scientific
7007452 CryoMed Freezer
6-3
Section 7
Parts List
Part Number
Description
Part Number
Description
103112
Removable Bulb Gasket (chamber opening)
4000384
Internal Straw Probe
195986
Silicone D-Gasket (door), 0.6 cu ft units
400152
Switcher 40W 12, 5, -12 V
195987
Silicone D-Gasket (door), 1.2 cu ft units
400148
200 F Manual Reset T-Stat
195988
Silicone D-Gasket (door), 1.7 cu ft units
430326
Cordset, 13A/125V, 16/3, USA
121065
Vise Action T-Handle Latch
430109
Cordset, 10A/250V, European
137017
Tubular Heater 945W, 115V
460180
Power Entry/RFI Line Filter
137018
Tubular Heater 945W, 230V
650012
Ext. Retaining Ring 0.250 Dia.
140296
Control Panel Overlay
730036
Teflon Washer
156123
1/4 Hp Blower Motor 1725
900134
Tubeaxial Fan, 30 cfm, 12V
190611
Stn Stl Hinge Pin
1890040
LN2 Inject Coil S-Asy
190612
Hinge Block Alum
1890049
LN2 Inlet Plumbing
191648
Display Board
1890050
LN2 Outlet Plumbing
191649
Control Board
4000401
6 ft. Transfer Hose
201183
Thermal Data Printer
4000617
120V Solenoid Valve
201185
120V Printer Power Supply
4000618
230V Solenoid Valve
201186
100V Printer Power Supply
4000619
LCD/Cable Assembly
201187
230V Printer Power Supply
4000621
Door Switch Assembly
201288
120V Printer Power Supply Cord
130059
Ampule Stopper
230184
15A DPDT Circuit Breaker/Switch
130060
Straw Stopper
230178
8A DPDT Circuit Breaker/Switch
4000566
Thermal Printer Paper, 5 rolls
251008
1/4 MPT Relief Valve 60 PSIG
270158
DB9 Cable, 10 ft.
290170
T-Type Chamber Probe
270159
DB25F to DB9M Adapter
370248
T/C Plug Type T
8028
Flatbed Chart Recorder
370249
T/C Socket Type T
4000404
PC Interface Software
4000385
Internal Sample Probe 1.2 ml
4000386
Internal Sample Probe 4/5 ml
Thermo Scientific
7007452 CryoMed Freezer
7-1
Section 7
Parts List
IVF Models Only
7-2
7007452 CryoMed Freezer
Part Number
Description
4000710
Straw Holder
4000711
Cane Holder
180254
Cane Holder Plug
180255
Access Port Plug
4000211
Cane
4000211
Cane, 1.2 ml, 6 ampule
180254
Cane Holder Plug
4000402
External Straw Sample Probe
4000403
External Vial Sample Probe
4000712
Sample T/C Vial with hole, 1.2 ml
4000713
Sample T/C Vial with hole, 2.0 ml
Thermo Scientific
Section 7
Parts List
Thermo Scientific
7007452 CryoMed Freezer
7-3
Section 7
Parts List
7-4
7007452 CryoMed Freezer
Thermo Scientific
Section 7
Parts List
Thermo Scientific
7007452 CryoMed Freezer
7-5
Section 7
Parts List
7-6
7007452 CryoMed Freezer
Thermo Scientific
Section 7
Parts List
Thermo Scientific
7007452 CryoMed Freezer
7-7
Section 7
Parts List
7-8
7007452 CryoMed Freezer
Thermo Scientific
Section 7
Parts List
Thermo Scientific
7007452 CryoMed Freezer
7-9
Section 7
Parts List
7-10
7007452 CryoMed Freezer
Thermo Scientific
Section 7
Parts List
Thermo Scientific
7007452 CryoMed Freezer
7-11
Section 7
Parts List
7-12
7007452 CryoMed Freezer
Thermo Scientific
Section 7
Parts List
Thermo Scientific
7007452 CryoMed Freezer
7-13
Section 7
Parts List
7-14
7007452 CryoMed Freezer
Thermo Scientific
Section 7
Parts List
Thermo Scientific
7007452 CryoMed Freezer
7-15
Section 7
Parts List
7-16
7007452 CryoMed Freezer
Thermo Scientific
Section 7
Parts List
Thermo Scientific
7007452 CryoMed Freezer
7-17
Section 7
Parts List
7-18
7007452 CryoMed Freezer
Thermo Scientific
Thermo Scientific
7007452 CryoMed Freezer
8-1
8-2
7007452 CryoMed Freezer
Thermo Scientific
Thermo Scientific
7007452 CryoMed Freezer
8-3
8-4
7007452 CryoMed Freezer
Thermo Scientific
Thermo Scientific
7007452 CryoMed Freezer
8-5
8-6
7007452 CryoMed Freezer
Thermo Scientific
Thermo Scientific
7007452 CryoMed Freezer
9-1
Heater Error Alarm
No Power-up “Splash” screen
Symptom
LED’s and displays not lighted
5). Replace controller board
(may also be power supply).
1). Adjust R8 for optimum
contrast.
4). DC outputs (5/12/-12VDC) not present when
power harness disconnected from controller board?
5). DC outputs (5/12/-12VDC) not present when
power harness connected to controller board?
1). If temperature displays and LED’s appear normal,
verify adjustment of R8 LCD contrast
4). Power supply
5). Control Board
3). LED’s and temperature display appear normal and
Pre-set profile #1 starts when the Run key is pressed.
4). Multiple LED’s lit (Idle/Run/Warm) and/or
unchanging misc. numbers in temperature displays.
1). Check thermal cutout.
3). LCD display
4). Controller board
1b). Replace controller
board
1b). If over limit or sensor failure logs present,
measure heater voltage on/off in manual mode. If on
but no off:
2). Measure more than 90/207VAC across heater in
manual mode with heater on and no chamber
temperature rise:
1b). Controller board
2). Heater
2). Replace heater
1a). Replace thermal cutout
1). Reset thermal cutout
4). Replace controller board
3). Replace LCD display (may
also be controller board)
2). Replace power supply (may
be controller board if components
seem hot to the touch)
3). Replace circuit breaker
1a). Examine Error Logs for “Chamber over limit
temperature” and/or sensor failure alarms. If none:
1). Heater Thermal Cutout
2). If +5V DC less than 4.75V when power harness
connected to controller board?
2). Power supply
1). LCD display contrast
4). Replace power supply
3). Measure less than 90/207VAC at power supply
input
3). Circuit Breaker
2). Replace power cord
2). Measure less than 90/207VAC at AC inlet?
2). Power cord
Solution
1). Correct problem
Verification
1). Measure less than 90/207VAC at outlet?
Possible Cause
1). Power at AC outlet
Troubleshooting Guide
9-2
7007452 CryoMed Freezer
Thermo Scientific
Fan will not run
Symptom
Check LN2 Supply Alarm
4). Replace solenoids
4). Check solenoid operation (sound & gas flow) in
manual mode and chamber temperature drop. If
more than 90/207VAC across solenoids and no sound
or temperature drop:
5). If less than 90/207VAC across solenoid (and AC
supply is adequate):
1). Check fuse/value
4). LN2 solenoids bad
5). Controller board bad
3). Controller board
2). Thermal cut-out in motor
(motor overheating)
1). Fuse on controller board
3). Replace/upgrade
hose/plumbing.
3). 4’/6’ flex line to tank or vacuum insulated
plumbing?
3). LN2 supply hose too long
3). Measure more than 90/207VAC across motor in
manual mode with motor on. If not:
3). Replace controller board
2a). Correct motor strapping
1d). Replace motor
1d). Check for excessive motor current and
120/230VAC strapping correct?
2a). 120/230VAC strapping in motor vs. AC supply
1c). Correct 120/230VAC
strapping.
1c). Check for excessive motor current vs. name
plate. 120/230VAC strapping incorrect? If not:
2). Correct AC supply problem
1b). Replace motor
1b). Check for free rotation of motor shaft.
2). Check if less than 90/207VAC across motor in
manual mode with fan on.
1a). Move/adjust to eliminate
obstruction.
1a). Check for unobstructed rotation of fan
1). Replace fuse
5). Replace controller board
2). Replace tank/regulator
2). Verify tank pressure (regulator: 22# nominal)
2). LN2 supply pressure too low
Solution
1). Replace/fill tank
Verification
1). Verify supply tank contents adequate?
Possible Cause
1). LN2 supply tank empty
Troubleshooting Guide
Thermo Scientific
7007452 CryoMed Freezer
9-3
Will not run selected profile
“ILL” on temperature display
Symptom
No temperature display (other
displays normal)
2). Confirm door is fully closed/latched (Run lit but
no fan)
3). Confirm resistance of door switch with door
closed is less than 10 ohms. If not:
4). Run Test and Software Checksum tests in
Configuration/Calibration – Service menu. If failures
presented:
3). Door switch
4). Controller board
1a). Confirm profile does not use Sample temperature
3). Short thermocouple inputs on controller board
with jumper wire or bent paper clip. If “ILL” display
still present:
1). “ILL” on Chamber or Sample temperature
2). Door open/ajar
1). Chamber/Sample probe
3). Controller board
2). Power supply
2). Verify 5/12/-12VDC at controller board power
connector. If not:
4). If controller overshoots 25C in Warm mode or
won’t initiate Warm mode:
1). Measure resistance between pins on probe harness
in sidecar for Chamber or Sample. If more than a few
ohms:
4). Controller board
1). Chamber or Sample probe,
connector screw, connector.
3). Confirm controller regulates at 25C by pressing
Warm key.
3). Display Board
4). Replace controller board
3). Replace door switch and/or
magnet
2). Close/latch door
1a). Remove sample temperature
from profile or add sample probe.
1). See “ILL” troubleshooting
3). Replace controller board
2). Replace power supply
1). Tighten connector screws,
replace probe, harness or
connector.
4). Replace controller board
3). Replace display board (may
also be controller board)
2). Replace power supply (may
also be controller board)
1a). Replace solenoid valves
1a). If solenoid voltage turned on/off in manual mode
but chamber still cold/cooling, confirm solenoid
valves stopping flow of LN2. If not:
2). Verify 12/-12VDC at controller board power
connector. If not:
Solution
1). Replace controller board
Verification
1). Display showing “+ _ _” or “-_ _” and chamber
appears to be beyond normal temperatures. Confirm
voltage on heater or solenoids (120/230VAC) can be
turned on/off by controller in manual mode. If not:
2). Power supply
Possible Cause
1). Chamber under/over limit
temperature
Troubleshooting Guide
9-4
7007452 CryoMed Freezer
Thermo Scientific
No recorder output
Symptom
No printer output
1b). Clear jam or replace printer
2). Select Chamber, Sample or
Scan
3). Turn power switch on (toward
rear of machine)
3a). Replace printer power
supply.
4). Reset printer DIP switches
1b). Online and Offline lights lighted on printer.
Check for paper jam. Do test print (power up while
holding feed button).
2). Check Configuration/Calibration – Printer menu,
set for “Off”?
3). Check “power” LED on front of printer. Confirm
power switch on left side of printer is on.
3a). Check voltage at printer power connector
(6.5VDC).
4). Confirm printer DIP SW-1, -2, and -3 correct
(power up while holding Online button).
2). Printer menu setup
3). Printer power
5). In Configuration/Calibration – Chart Recorder
5). Controller board
3). In Configuration/Calibration – Chart Recorder
menu, set recorder for +50C and measure approx.
9.7V at chart recorder connector on controller board.
If not present at recorder:
3). Recorder cable/connection
4). In Configuration/Calibration – Chart Recorder
menu, set recorder for +50C and measure approx.
9.7V at posts on side of recorder. If present:
2). Check Configuration/Calibration – Chart
Recorder menu, set for “Off”?
2). Recorder menu setup
4). Recorder
1). Confirm recorder power light lighted
1). Recorder power
6). Controller board
5). Printer harness
4). Repair/replace recorder
3). Replace recorder cable or
recorder harness inside CRF.
2). Select Chamber, Sample or
Scan.
1). Correct AC supply, power
cord, power switch.
1a). Replace thermal paper
1a). Offline light flashing. Check paper.
4). Internal printer setup
Solution
1). Press Online button on printer
Verification
1). Confirm green Online light lighted on printer
Possible Cause
1). Printer not “on line”
Troubleshooting Guide
Thermo Scientific
7007452 CryoMed Freezer
9-5
Chamber temperature erratic
1). Verify tank pressure (regulator: 22# nominal)
2). Verify thermocouple head out of mounting tube ¾
inch
3). Verify correct PID values in Service – Thermal
Controller menu
4). Test for short and verify position.
1). High LN2 pressure
2). Chamber thermocouple
mounting
3). Customer adjusted PID
parameters
4). T/C shorted or incorrectly
positioned.
Troubleshooting Guide
3). Set correct PID values or
reset to factory defaults in the
Service menu (Make note of
probe calibration values prior to
reset to factory defaults or recalibrate probes as necessary)
2). Adjust thermocouple head
position.
1). Replace tank/regulator
Section 10
PC Interface Software
Section 10
PC Interface Software
Figure 10-1. Opening Screen
The CryoMed Controlled Rate Freezer (CRF) includes the option for PC
interface. The interface requires one of the following operating systems:
MS Windows 95, 98, ME, NT, XP, or 2000.
The PC software can create and download freezer profiles. It can initiate a
profile run, monitor its progress, then create a printed report summary of
the run data. During the time that the freezer is running a PC-initiated
profile (Remote Run), the control panel keypad is disabled with the
exception of the Silence key.
The PC software communicates with the CRF through the RS-232 serial
connection (included with the freezer is a 10' male-female DB9 cable and
a DB25F to DB9M adapter). Data information is routed with a check
byte for transmission integrity. The software stays in continual touch with
the freezer to detect any communication breakdown.
A single PC can communicate with up to four Controlled Rate Freezers,
using separate Run windows. To connect a PC to more than one CRF,
install a multiple port serial interface card in the PC. Interface cards should
meet the following specifications.
•
•
•
•
•
Thermo Scientific
9-pin RS232 ports (1 communication port for each freezer)
Support baud rates up to 9600K baud minimum
16550 UARTS (suggested)
128 byte transmit and receive buffers (FIFOs, suggested)
Automatic assignment of ports (suggested)
7007452 CryoMed Freezer
10-1
Section 10
PC Interface Software
Installation & Set-Up
Make sure the cable between the Controlled Rate Freezer and the PC is
connected properly.
To install and set-up the PC software, locate and execute the Set-Up.exe
file on the software CD by selecting Start, then Run. The software is
installed in the default directory. Additional directories are created for the
freezer profiles (Programs) and run data (Run Files).
The PC software must be registered. If, after 30 days, the software has not
been registered, an alert screen will display on start-up. The registration
passcode is T2YRPW9PWFXD28GJ
When installation is complete, click the Option button to set default
directories, information fields and add user names and passwords, if
password protection of all Main Menu features is desired.
System software (Lab View Run Time Engine) is installed along with the
PC interface software.
Note This system software is required for proper operation of the PC
interface with the freezer. ▲
Main Menu
As the PC interface program begins, a splash screen (Figure 10-1) appears
just before the registration screen. The splash screen contains the company
logo, contact information and a picture of the Controlled Rate Freezer.
The Main Menu buttons are displayed; Edit, Run, Review, Options, and
About. Selected button turns dark when activated.
10-2
7007452 CryoMed Freezer
•
The About screen displays the Thermo family of laboratory equipment
plus the company Mission statement. Click anywhere on this screen to
close it.
•
The Exit button closes the PC interface application software. All other
windows must be closed before exiting to avoid error messages.
Thermo Scientific
Section 10
PC Interface Software
Edit Screen
Figure 10-2. Freezing Profile
On the Edit screen, a freezing profile can be created and saved by the
operator. An existing profile may be edited, if required. A profile listing
can be printed. An approximate representation of the freezing profile will
be displayed in the Edit window graph.
These are the profile steps available in the pull-down menu with
definitions following.
Step Name with Menu Format
Step Parameters with Definitions
Wait - Wait At X.X°C
where X.X is the chamber set
temperature
Maintains specified chamber
temperature indefinitely until the
user clicks the "Program waiting for
user -- Click here to continue"
button on the Run screen. The
temperature can be set over a range
of -180°C to +50°C with a setting
resolution of 0.1ºC.
Thermo Scientific
7007452 CryoMed Freezer
10-3
Section 10
PC Interface Software
Edit Screen
(continued)
Figure 10-3. Wait for Temp
Step Name with Menu Format
Step Parameters with Definitions
Wait for temperature WAIT AT CHAMBER=X.X°C UNTIL SAMPLE=Y.Y°C
where X.X is Chamber set temp
and Y.Y is the Sample trip temp.
The default Sample trip temp is1°C
above Chamber temp.
Step maintains specified Chamber
temp and moves to next step in
profile when Sample temp cools
to the specified temp. This step can
only be used when the starting
Sample temp is greater than the
specified Sample temp (the Sample
is cooling).
Note The sample temp must be greater than the chamber temp. ▲
10-4
7007452 CryoMed Freezer
Thermo Scientific
Section 10
PC Interface Software
Edit Screen
(continued)
Figure 10-4. Ramp Rate
Step Name with Menu Format
Step Parameters with Definitions
Ramp - RAMP X.X°C/MIN UNTIL CHAMBER=Y.Y°C
RAMP X.X°C/MIN UNTIL SAMPLE=Y.Y°C
or
where X.X is the ramp rate and Y.Y
is the chamber (or sample) endpoint
temperature.
Changes Chamber temp from its
current value to specified temp
(Chamber sensor trip point) at the
selected rate (°C/minute). The rate
can be set over a range of 0.1°C/
minute to 99.9°C/minute. The
temp can be set over a range of
-180°C to +50ºC(±0.1ºC). When
the Chamber reaches specified
temp, the control moves to the next
step in the profile. If Sample sensor
is selected, Chamber temp changes
at a specified rate until Sample temp
reaches specified temperature.
Note The slope of the temperature ramp is determined automatically,
based on the temperature entered and the chamber temperature at the
beginning of the step. Entry of a sign for the rate term is not
required/allowed. ▲
Thermo Scientific
7007452 CryoMed Freezer
10-5
Section 10
PC Interface Software
Edit Screen
(continued)
Figure 10-5. Hold at Temperature
Step Name with Menu Format
Step Parameters with Definitions
Hold - HOLD X.X°C FOR Y.Y MINUTES
where X.X is the chamber set
temperature and Y.Y is the minutes
Maintains a specified Chamber
temperature for the specified hold
duration before proceeding to the
next step. The time can be set over a
range of 1 to 99 minutes, and the
temperature can be set over a range
of -180°C to +50°C with a setting
resolution of 0.1°C.
Figure 10-6. Jump to Specified Step
Jump - JUMP TO STEP X
where X is the step number
Automatic advance to a subsequent
step or return to a previous step in a
freezing profile. The Jump function
can be programmed over a range of
Step 1 to Step 20.
10-6
7007452 CryoMed Freezer
Thermo Scientific
Section 10
PC Interface Software
Edit Screen
(continued)
Figure 10-7. Repetitions From Specified Step
Step Name with Menu Format
Step Parameters with Definitions
Loop - LOOP X TIMES STARTING AT STEP Y
where X is number of repetitions
and Y is the step number to begin
Automatic advance to a subsequent
step or return to a previous step in a
freezing profile for a programmable
number of repetitions. The Loop
function can be programmed over a
range of Step 1 to Step 20, and over
a range of repetitions from 1 to 99.
End
Last step in profile
Maintains the last Chamber
temperature from the previous step
indefinitely until the user terminates
the Run with the Stop button or
from the freezer keypad.
Create a New Freezer
Profile
Follow the procedure below to create a new freezing profile.
1. Select the desired first step type from the Step pull down menu. WAIT
or WAIT FOR TEMPERATURE is the mandatory first step. It is
required to purge the warm LN2 plumbing prior to initiating
controlled rate freezing. This step cannot be deleted or changed to a
different step type.
2. Select subsequent steps. Use Insert to add a step. The new step is
added on the line that is highlighted. Use Replace to modify an
existing step's parameters or change to a different step type.
3. END is the mandatory last step. It alerts the freezer that the profile
steps are completed. This step is automatically added during the profile
creation. It cannot be deleted or replaced.
Thermo Scientific
7007452 CryoMed Freezer
10-7
Section 10
PC Interface Software
Create a New Freezer
Profile (continued)
4. Save the profile by selecting the Save button. Enter a profile name and
Save. A comment may be saved with the profile. Comments are printed
with the Run data. Saved profiles are given the extension .prg.
5. Select the Delete All button to clear the program listing window.
6. There can be a maximum of 20 steps, including the End step.
Edit an Existing Profile
A saved or already existing profile may be changed as needed.
1. Open a freezing profile by selecting the Open button. The steps appear
in the program listing window.
2. Select the step to edit. Change the step type or modify the step
parameters and click Replace.
3. Choose the point at which to insert a new step.
4. New steps are inserted at the position highlighted in the program
listing window.
5. Subsequent steps move down one position as new ones are added.
6. Select the step type and enter the desired parameters.
7. Press the Insert button to insert the new step. Use the Replace button
to modify or edit a step. Use the Delete button to delete a step or the
Delete All button to delete all the steps in the profile.
8. Save the edited profile. The same file name may be used or a new
profile name can be entered. An optional comment may be saved with
the profile. Comments are printed with the Run data.
Print a Profile Listing
10-8
7007452 CryoMed Freezer
A listing of the open freezing profiles may be printed at any time by
selecting the Print button. Print requests are sent to the Windows default
printer. To choose a different default printer, first exit the interface
software. Choose the printer, then restart the PC interface software. The
printer selection will not be changed if the software application is still open
at the time of the change.
Thermo Scientific
Section 10
PC Interface Software
Display Profile Temp
Graph
A representation of the freezing profile is displayed on the Edit window
graph. Refer to the illustration following. The starting point of each Step is
indicated on the graph line by a small square. The graph can be moved
horizontally and vertically by using the scroll buttons. Scale adjustments
can be made using the time and temperature pull-down menus.
Figure 10-8. Edit Window Graph
Error Messages
If selected step parameters are out of range, an error message will appear
when the Enter or Replace button is pressed. Press OK and correct the
parameters.
When saving a profile which has the same name as one already existing, a
file overwrite error message will appear. Choose YES to overwrite (replace)
or NO to enter a new file name.
Thermo Scientific
7007452 CryoMed Freezer
10-9
Section 10
PC Interface Software
Run Screen
User Control Definitions
•
Data Tag is mandatory information entered by the user which is used
as a prefix for the file name in which the run data is stored. Note: If
illegal characters (/ \ : * ? < > |, all lower case word “run”) are used in
the data tag, the program runs normally but no data file is created.
•
Date/Time is information taken from the PC system clock and stored
with the run data.
•
User Name is supplied by the security module of the software.
‘Anonymous’ is used if password security is not enabled.
•
Run window status bar, prior to the start of a run, shows the
communication port, the profile file name, and the path of the profile
selected. After the start of a run, the bar shows the communication
port, the profile name, and the run data storage file name.
•
The Start button initiates a Run. This button changes to 'Move to'
during a Run when a different step is selected in the Step pull-down
menu.
•
The Print button initiates a print-out during a Run or after a Run is
complete, using the Windows default printer. Color or monochrome
output may be selected.
a. If default printer is monochrome, select 'Monochrome'.
b. If the default is color, select 'Monochrome' or 'Color'.
Choosing 'Monochrome' on a color printer uses marker/line
patterns to identify different lines on the graph (i.e. Sample
temperature, Chamber temperature, Profile, etc).
If 'Color' is selected with a monochrome printer, colors are
represented by shades of gray so some colors may not be clearly
visible.
c. Selecting 'Single page report' prints the current Run screen.
10-10
7007452 CryoMed Freezer
Thermo Scientific
Section 10
PC Interface Software
User Control Definitions
(continued)
d. Selecting 'Two page report' prints an information sheet first, which
includes the following;
•
•
•
•
•
•
•
•
Profile name and path
Run data file and path
User name
Date/time
User Information Field name/contents
Run start/stop "comments"
Profile listing
Alarm listing with time of occurrence
The second page printed is the current screen graph display.
e. Selecting 'Multi-page report' prints the information sheet described
above as the first page.
The pages following are the entire Run graph, printed vertically for
best presentation. Horizontal scale may be selected on the Run
screen. The number of pages to be printed in horizontal scale is
indicated on the print-out selection window.
•
Stop/Exit button stops a Run in progress, or exits the Run screen after
a Run has ended. The Run screen may be exited during Warm.
However, the Controlled Rate Freezer will maintain Warm Mode until
terminated by the user at the keypad.
•
Auto-Scroll button is an On/Off button with the default being On.
This button has an automatic adjustment of the graph scale so that it
is always visible in the window. In addition, the most recent data is
automatically displayed.
•
The Warm button appears when a Run is terminated. It allows the user
to initiate a Chamber Warm from the PC. This button has an On or
Off option. Click once to turn On; click again to turn Off.
•
Manual override buttons are activated by pressing and holding the
mouse button. When the mouse button is released, the function is
deactivated. This button is only available during a profile Run.
a. The override Heat button turns the heater on.
b. The override Cool button turns the Cool solenoid on.
c. The override Cool+ button turns the Cool and Cool+ solenoids
on.
Thermo Scientific
7007452 CryoMed Freezer
10-11
Section 10
PC Interface Software
User Control Definitions
(continued)
•
Time/Temperature Division pull-down menus are as follows:
a. Temperature divisions may be chosen by clicking the down arrow
to select 20/10/5/2/1°C per division.
b. Time divisions may be chosen by clicking the down arrow to select
20/10/5/2/1/0.5/0.1 minutes per division.
•
The Program Listing pull-down menu shows all the steps in the current
profile. A check mark indicates the step that is currently running. This
menu can be used to manually move the freezer to a different step
(forward or backward). Select the step destination. It turns yellow to
indicate the change is not yet complete. When a different step is
selected, the 'Start' button changes to 'Move to'. Select it to execute the
move. The selected step turns white to show the move is complete and
the new step is running.
To start a Run at any step after the first step, choose the step to begin.
Then select the 'Start' button to begin the run. The step turns white to
show the change is complete. The Run starts at the chosen step.
•
Chamber/Sample temperature window indicates these temperatures at
a chosen point on the graph. To choose a point on the graph, first
place the mouse pointer on the default cursor position (left side of the
on-screen graph /yellow vertical line). Press and hold the left mouse
button and drag the curser to the desired point on the graph.
•
Graph legend selection box includes the legend which shows the
colors/plot styles of the on-screen plot lines. A variety of colors/styles
may be selected for the current window only. The default colors are
listed in the Options/Plot Colors window. Any changes made are in
effect for the current window only.
To change the color/pattern, click the plot line to be changed within
the graph legend box. Select the Color, Line Style or Line Width from
the pull-down menu. An on screen plot line may be rendered invisible
by selecting a color that matches the on-screen background color. A
white color will make the plot line invisible on color print-out.
•
10-12
7007452 CryoMed Freezer
Scroll the Graph from side-to-side, using the right/left arrow buttons.
Use the up/down arrow buttons to scroll the graph top-to-bottom.
Turn Auto-Scroll off to view a portion of the graph off-screen from the
current plot position.
Thermo Scientific
Section 10
PC Interface Software
Status Indicators
The Controlled Rate Freezer remote status indicator messages are listed
and defined below. The indicator status is valid 1-5 seconds after the Run
screen opens.
•
CRF Idle: Freezer is in the Idle state
•
CRF Warming: Freezer is in the Warm mode
•
CRF Busy: Run screen was opened for a freezer currently running a
stand-alone profile
•
Remote Mode: Freezer is running under control of the PC
•
CRF Running Stand-alone: Freezer started a Remote Run under PC
control but is now running the remote profile off-line. This is caused
by a PC-to-CRF communication interruption of more than 20
seconds.
The current Chamber/Sample temperature indicators show the present
temperatures at the freezer.
•
Operating Indicators
ILL: Indicates an open Sample or Chamber thermocouple probe
The Operating Status indicators are as follows:
•
Idle: CRF is in idle mode; fan solenoids and heater turned off
•
Run: Running a profile
•
Wait: Running a profile but waiting for user intervention
•
End: Running a profile but at the End (last) step in the profile
•
Warm: Warming the chamber to 25C
Pop-up status windows display a message requiring user intervention.
During a profile ‘Wait’ step, a message will appear ‘Program waiting for
user -- Click here to continue’. Click on the message box to continue to
the next step in the profile.
Alarm messages may also appear before or during a run. These messages
alert the user to the alarm status of the freezer. The user must clear the
message on the screen even though the alarm condition may have already
corrected itself.
Thermo Scientific
7007452 CryoMed Freezer
10-13
Section 10
PC Interface Software
Operating Indicators
(continued)
For more information on the alarms listed below, see the Controlled Rate
Freezer manual.
•
Temperature High (tracking)
•
Temperature Low (tracking)
•
Power Fail and failure duration
•
Chamber Sensor Failure
•
Sample Sensor Failure
•
Chamber Limit Temperature
•
Sample Limit Temperature
•
Heater Malfunction
•
LN2 Supply
•
Run Complete
•
Warm Complete
•
CRF Printer Buffer Full
When an alarm condition occurs, sudible and visual alarms are presented at
the freezer, and an alarm window appears on the PC screen. The window
contains a list of up to three of the most recent alarms and the message
‘Click Here To Silence’. Click anywhere on the alarm window to silence
the audible alarm at the freezer. After the audible alarm is silenced, another
alarm window appears, showing the list of up to three most recent alarm
and the message ‘Click Here To Clear’. Click anywhere on the alarm
window to clear all alarms whose conditions have been resolved.
The alarm window reappears if any alarms are still active. Correct these
conditions and click anywhere on the alarm window to clear these alarms.
If the message ‘Profile Stopped At Freezer’ displays, the run has been
stopped by a component failure in the freezer, or by the user pressing the
Back button at the freezer while the profile is in the End step. Click on the
message to acknowledge that the run was stopped. This action has the
same effect as clicking the Stop button.
10-14
7007452 CryoMed Freezer
Thermo Scientific
Section 10
PC Interface Software
Typical Run
Procedure
1. Click the Run button on the Main screen.
2. Select the PC commmunication port. Available ports are shown on a
pull-down menu. If a port is in use by another freezer, the port will
not appear on the menu. Click ‘Continue’, or ‘Cancel’ to abort start.
3. Select a freezing profile. The File Select window opens at the default
directory chosen in the Options screen. Choose a file, or change
directories, then choose a file. Click ‘Open’ to continue or ‘Cancel’ to
abort the start of the Run. As the Controlled Rate Freezer goes into
‘Remote Mode’ and profile is downloaded and validated, then the
freezer keypad is disabled except for the Silence key.
4. Enter the mandatory ‘Data Tag’. Select a word or abbreviation to
identify the Run. The Data Tag is used as Run data file name prefix,
followed by a sequential number generated by the application. The
Data Tag information must be entered before the Run can be started.
Note: If illegal characters (/ \ : * ? < > |) are used in the data tag, the
program runs normally but no data file is created.
5. Enter any optional data in previously enabled ‘Information Fields’. Up
to 12 fields can be enabled and named in the Options screen. The
information entered is captured in the Run file and displayed on the
Review screen and information page print-outs.
6. Select the starting step of the profile. Default is the first step in the
profile. Any step can be chosen to begin the profile.
7. Initiate the profile Run by clicking the Start button.
8. Enter optional comments. The text entry window opens for the option
of adding, for example, notes pertaining to the Run or samples. Click
‘Continue’ to begin capturing temperature data from the Controlled
Rate Freezer. Run data will not be captured until the comment
window is closed.
9. Stop the freezer after the completion of the run and removal of the
samples. The freezer holds the last chamber temperature indefinitely at
the End step. The Run may be stopped by clicking on the Stop button
or pressing the Back key on the freezer keypad.
10. Enter any additional comments when the text entry window opens
again. This window appears regardless of the Run stop method.
Thermo Scientific
7007452 CryoMed Freezer
10-15
Section 10
PC Interface Software
Typical Run
Procedure (cont.)
11. Initiate Warm Mode. The Warm button appears after the Run stops
and the comment window is closed. Click the Warm button, or press
the Warm key on the freezer keypad. The PC monitors the freezer’s
warming status.
12. Terminate Warm Mode and close the Run screen with Exit button.
Warm Mode can be terminated by clicking the Warm button, or by
pressing the Back key on the freezer keypad. The PC monitors the
Warm status regardless of the method of termination.
The Run screen may be closed without terminating the Warm mode by
clicking the ‘Exit’ button. The freezer continues to warm until it is
either stopped by the user at the freezer keypad, or automatically 30
minutes after the Warm Complete alarm occurs.
Power Failure
Recovery
If the Controlled Rate Freezer experiences a power failure and the PC
continues to operate:
When power is restored, the freezer will continue the Run at the point
of failure. However, the freezer runs in ‘Stand-alone’ mode. All
commands from the PC are ignored except for profile status requests.
The temperature data is continually captured and plotted. The PC
cannot clear alarms or stop the Run. The freezer’s keypad remains
disabled except for the Silence key, which may be used to mute or clear
any alarms. The Run can be aborted at the freezer by pressing and
holding the Back key for 3 seconds. The PC will then resume control
of the freezer. Pressing the Stop button on the Run screen ends data
capture only. The PC will report a Power Fail alarm including the
failure duration, if the failure duration was longer than 10 seconds.
The graph on the Run screen will show a temperature occurrence of
zero to indicate a freezer power failure, rather than a PC-only power
failure. A communication error message will display on the Run screen
during the freezer power failure.
10-16
7007452 CryoMed Freezer
Thermo Scientific
Section 10
PC Interface Software
Power Failure
Recovery (continued)
If the PC experiences a power failure and the Controlled Rate Freezer
continues to operate:
The freezer switches to ‘Stand-alone’ mode if there is no
communication from the PC for more than 20 seconds. When power
is restored to the PC and Windows reboots, restart the freezer
application. Select Run from the Main Menu. Choose the COM port
for the freezer that is running. The PC will recover the Run data
captured prior to its power failure, then continue the Run in ‘Standalone’ mode. The graph on the Run screen will show the gap when the
PC was not communicating. Data not saved prior to the power failure
cannot be recovered. It could be as much as 1 minute of Run data, as
the application saves data to disk approximately once per minute. Up
to four Run screens can be restored for freezers that were running
during the PC power failure. As the freezer did not experience the
power failure, no alarm will be recorded.
If both the freezer and the PC have power failures, see the previous
paragraph. The behavior and recovery are the same as above.
Error Messages
•
‘Communication Failure -- CRF Not Responding’
This message indicates that the freezer is not sending status updates to
the PC when requested. Verify that the communication cable is
connected and working correctly, the freezer has power and is turned
on, and that there has not hardware failure in the PC or freezer. If
communication is interrupted for more than 20 seconds, the freezer
will go into ‘Off-line’ mode.
•
‘Freezer Already In Use’
This message appears when a
communication port is
selected for a freezer that is
running a profile initiated
from the freezer keypad. In
this case, the PC cannot take
control. Select the ‘Terminate
Session’ button to close the
Run window.
Thermo Scientific
7007452 CryoMed Freezer
10-17
Section 10
PC Interface Software
Error Messages
(continued)
•
‘Cannot Stop Freezer in Stand-alone Mode’
This message displays if the Stop button is clicked while the freezer is
running a Remote profile in ‘Stand-alone’ mode. Clicking the Stop
button at this time stops only the data capture. Choose ‘Continue
capturing data’ or ‘Stop capturing data’. No other commands can be
sent from the PC.
Display/Control
Summary
Three modes are available when using a Controlled Rate Freezer and PC
configuration: Remote Mode, Stand Alone Mode and Running a Local
Profile.
Remote Mode
The PC can initiate all actions at the freezer such as Run, Stop, Warm,
Manual Over-ride, Alarm Silence and Alarm Clear. The PC displays the
current freezer operation status (Idle, Run, Wait, End, Warm) and the
chamber and sample temperatures. Time and temperature details are
captured every 6 seconds.
The CRF keypad is disabled except for the Silence key which can be used
to silence the audible alarm and clear alarm messages at the freezer. Alarm
messages on the PC must be cleared at the PC as an added assurance that
the PC user will be aware of the alarm condition. The other freezer keypad
keys are disabled to prevent persons near the freezer from taking control of
a freezing run without the knowledge of the user/initiator of the run. All
freezer status and temperature displays operate normally. The message
'Under Remote Control' displays on the freezer as well as the current
profile step being executed. Alarm messages alternate with the current step
information if an alarm condition occurs.
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Thermo Scientific
Section 10
PC Interface Software
Stand Alone Mode
The PC can only capture time/temperature and alarm data in this mode.
No action commands are permitted. Current operating status and
chamber/sample temperatures will be updated and displayed normally.
The freezer keypad is still disabled in this mode except for the Silence key.
However, a run may be aborted by pressing and holding the Back key on
the freezer for 3 seconds. All freezer status and temperature displays
operate normally. The message 'Running Remote Profile' is displayed at
the freezer, as well as the current step information alternated with any
alarm messages.
Running a Local Profile
Any attempt to open a Run screen for a freezer already running a local
profile will result in an error message ‘Freezer Already In Use’. The Run
screen cannot be opened.
Review Screen
Refer to the details below concerning the information fields and user
controls for the review screen.
Information Fields
•
Data Tag: Shows mandatory information entered by the user for the
open Run file
•
Date/Time: Shows the start time/date of the open Run file
•
Operator: Shows user name, or "Anonymous" if no users are set up in
Options screen
•
Review window status bar: Shows the name and path of open Run file
•
Review step select pull-down menu: Displays the steps of the profile
in the open ‘Review’ Run file
•
Compare step select pull-down menu: Displays the steps of the profile
in the open ‘Compare’ Run file
Thermo Scientific
7007452 CryoMed Freezer
10-19
Section 10
PC Interface Software
Review Screen
(continued)
User Controls
•
Review Button
This button opens an existing Run profile for on-screen review and
print-out. The Chamber and Sample temperatures can be examined
over the time, or the actual Chamber temperature may be compared
with the programmed temperature. Click the Review button again to
open a different file.
•
Compare Button
This button opens a second file for comparison. Both files are plotted
on the graph. Click the Compare button again to remove the
comparison data.
•
Print Button
This button is used to initiate a print-out of the Review and Compare
data, using the Windows default printer. Select color or monochrome
(black & white) output. If the printer is monochrome, select
‘monochrome’. If color, select either. Monochrome uses marker/line
patterns to identify specific lines on the graph. If ‘Color’ is selected
with a monochrome printer, colors are represented by shades of grey
(some colors may not be clearly visible).
10-20
7007452 CryoMed Freezer
Thermo Scientific
Section 10
PC Interface Software
User Controls (continued)
‘Single Page Report’ may be selected to print the current Review
screen. ‘Two Page Report’ may chosen to print, in addition to the
current view of the graph, one or two information sheets. The first
sheet printed on a two page report is the open Review file information.
1) Review file profile name and path
2) Review Run data file and path
3) User name
4) Date/time
5) User Information Field name/contents
6) Run start/stop "comments"
7) Profile listing
8) Alarm listing with time of occurrence
If a Compare file is open, a second information sheet is printed.
‘Multi-page Report’ prints information pages on the Review profile,
and the Compare profile, if open. Subsequent pages are the entire Run
graph. Horizontal scale is as selected on the Review screen. This also
determines the number of pages, as indicated in the print-out selection
window. Vertical scale may be automatically selected for best
presentation.
1) Compare file Profile name and path
2) Compare Run data file and path
3) User name
4) Date/time
5) User Information Field name/contents
6) Run start/stop "comments"
7) Profile listing
8) Alarm listing with time of occurrence
Thermo Scientific
7007452 CryoMed Freezer
10-21
Section 10
PC Interface Software
User Controls (continued)
•
Exit Button
This button may be used to exit the Review screen. It is not necessary
to close any files before exiting.
•
Export Button
This button may be used to export the open Run profile data into a
‘comma-separated variable’ format. The data can then be imported into
standard spreadsheet or database programs.
The data includes the data tag, date/time, user name, information field
name and contents, source data file name, profile steps, elapsed time,
profile temp, Chamber Temp, Sample temp (run data), and any alarm
with occurance time.
•
Scale Select Pull-down Menus
Temperature division: Click down arrow to select 20/10/5/2/1ºC per
division
Time division: Click down arrow to select 20/10/5/2/1/.5/.1 minutes
per division
Graph cursor and Chamber/Sample temperature windows used to
indicate the Chamber/Sample temperature at a selected point on the
graph. The default cursor position is the left side of the on-screen
graph (yellow vertical line). Point at cursor, press and hold the left
mouse button and drag the cursor to the desired graph position.
•
Graph color selection box
This box includes a graph legend showing the colors/plot styles of onscreen plot lines. Select from a variety of colors/styles for the current
window only. Default colors are as specified in the Options/Plot Colors
window. Any color/pattern changes are in effect for the current
window.
To choose colors/patterns, left-click the plot line to be changed within
the legend box. Select the color, line style or line width from the pulldown menu. Selection of an on-screen background color can make the
plot line invisible on the graph. White can be selected to make the plot
line invisible on print-out (color print-out).
•
Graph Scroll Buttons
The left/right scroll arrow buttons move the graph right or left in the
display window. The up/down scroll arrow buttons move the graph up
or down in the display window.
10-22
7007452 CryoMed Freezer
Thermo Scientific
Section 10
PC Interface Software
Options
Plot Color Selections
Several options are available. See the tabs in the illustration below.
The plot colors must be chosen so as to be distinguishable from each other
on the screen and in the print-outs. Make sure there is a noticeable
difference between the Chamber and Sample colors. Choose plot colors for
the Chamber temp, Sample temp, Profile, Alarm, steps, background and
grid. Save before leaving the Options screen.
To choose colors, click on the color box corresponding to the desired plot
item. Move the cursor over the desired color (observe the changes in the
sample graph). Click the palette button to create custom colors. When the
cursor is over the desired color, click the left mouse button. Click Save to
keep changes. Click Restore to return to previously saved colors. Click Exit
to exit Options menu.
Thermo Scientific
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10-23
Section 10
PC Interface Software
Information Field Set-Up
Select the number of information fields to be displayed on the Run screen.
Information field names and contents are saved with the Run data file for
each enabled field. Be sure to save choices before leaving the Information
Fields screen.
Click the check box next to a field to enable it. Enter a name for each
enabled information field. Click Save to keep the changes. Click Restore
to return to previously saved changes. Click Exit to exit the Options
screen.
Default Directories
Directories may be set up to store program (profiles) and run files. Save
before leaving the Default Directories screen.
Enter the desired path in the window for the default program directory
and run data directory. A Windows directory browse button may also be
selected. Click Save to keep changes. Click Restore to return to previously
saved changes. Click Exit to exit Options screen.
10-24
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Thermo Scientific
Section 10
PC Interface Software
User Name and Password
Maintenance
User names and passwords may be set up to protect all entries into the
Edit, Run, Review and Options menus. Save before leaving the Users
screen.
1. Type a name into the User Name window.
2. Type the desired password in the Password window.
3. Confirm the password in the Confirm Password window.
4. Click Add button to add the user name and password to the list.
5. Repeat for any additional user names.
6. To change a password, select the user name from the pull-down list (or
type the name in User Name window), enter the new password and
click the Modify button.
7. To delete a user name, select the user name form the pull-down list (or
type name in User Name window) and click the Delete button.
8. Click Save to keep changes.
9. Click Restore to return to previously saved changes.
10. Click Exit to exit Options screen.
If no user names are defined, all menus can be accessed without password
protection. Anonymous is listed as the user name if no users are set up. If
password protection is no longer desired, simply delete all user names.
Thermo Scientific
7007452 CryoMed Freezer
10-25
Section 10
PC Interface Software
Saving Changes
Sharing Host PC
Resources
Each of the four screens described above must be saved individually. The
application will prompt to Save if unsaved changes are present when the
Exit button is clicked.
When using the same PC for several freezers, potential problems could
occur during printing. There could be a loss of Run data points. Chart
data is updated every 6 seconds. Run data is captured every 6 seconds with
updates to hard disk every minute. Serial port communication may stop
for a short time during printing. The loss of a small number of data points
during slow ramps or holds will not cause a problem (chart lines are
interpolated across the gaps). Loss of data during fast ramps may be more
critical. Experiment with test runs before trying to print during a critical
part of a critical freeze. If in doubt, don't print during a critical run.
If, because of printing, the freezer loses communication for more than 20
seconds, the freezer will go in ‘Stand Alone’ mode. A very slow PC
(<166MHz) with limited resources (<32MB RAM) may stop serial
communication for more than 20 sec during printing. In ‘Stand Alone’
mode, PC control is disabled and there may be loss of run data for the
duration of the serial communication interruption. Experiment with test
runs, or if in doubt, don't print during a critical run.
Using other applications on the same PC with a Run in progress may cause
the loss of Run data points. Serial port communication may stop for a
short time if PC resources are consumed by other applications. Chart lines
continue across the communication gaps. Slow ramps or holds will lose
very little data. However, steep ramps may be more critical. Experiment
with test runs, or if in doubt, don't run other applications during a critical
profile run.
If there is a loss of communication between the PC and the freezer for
more than 20 seconds, the freezer may go into ‘Stand- Alone’ mode. A
slow PC (<166MHz) with limited resources (<32MB RAM) may stop
serial communication for more than 20 sec if multi-tasking. PC control
would be disabled for the duration of the current Run. Run data is lost
during the time of the communication interruption. Experiment with test
runs, or if in doubt, don't run other applications during a critical profile
run.
A Windows crash may stop serial communication for more than 20
seconds. The freezer will go into ‘Stand Alone’ mode and PC control will
be disabled for the duration of the current Run. Run data will be lost
during the time of the communication interruption. Experiment with test
runs, or if in doubt, don't run other applications during a critical profile
run.
10-26
7007452 CryoMed Freezer
Thermo Scientific
Thermo Scientific
7007452 CryoMed Freezer
11-1
Rev. 2 5/07
REGISTERED
ISO
9001
If equipment service is required, please call your Technical Services Department at 1-888-213-1790 (USA and Canada) or
1-740-373-4763. We're ready to answer your questions on equipment warranty, operation, maintenance, service and special
application. Outside the USA, contact your local distributor for warranty information.
Thermo shall not be liable for any indirect or consequential damages including, without limitation, damages relating to lost
profits or loss of products.
The Technical Services Department may be contacted for set-up and operational questions on the Controlled Rate Freezer
and PC interface, 1-888-213-1790. Printed instruction manuals carefully detail equipment installation, operation and preventive maintenance.
The warranty on any of the computer components must be referred to the component manufacturer. Please contact them as
needed.
THERMO FISHER SCIENTIFIC PC AND PERIPHERAL WARRANTY
11-2
7007452 CryoMed Freezer
Thermo Scientific
Rev. 2 5/07
REGISTERED
ISO
9001
If equipment service is required, please call your Thermo Services Department at 1-888-213-1790 (USA or Canada), or
1-740-373-4763. We're ready to answer your questions on equipment warranty, operation, maintenance, service and special application. Outside the USA, contact your local distributor for warranty information.
Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment
arrives. Printed instruction manuals carefully detail equipment installation, operation and preventive maintenance.
Thermo shall not be liable for any indirect or consequential damages including, without limitation, damages relating to lost
profits or loss of products.
The Technical Services Department may be contacted for set-up and operational questions on the Controlled Rate Freezer
and PC interface, 1-888-213-1790. Printed instruction manuals carefully detail equipment installation, operation and preventive maintenance.
The Warranty on any of the computer components must be referred to the component manufacturer. Please contact them
as needed.
THERMO FISHER SCIENTIFIC INTERNATIONAL DEALER
PC AND PERIPHERALS WARRANTY
Thermo Scientific
7007452 CryoMed Freezer
11-3
Rev. 2 2/07
REGISTERED
ISO
9001
If equipment service is required, please call your Technical Services Department at 1-888-213-1790 (USA and Canada) or
1-740-373-4763. We're ready to answer your questions on equipment warranty, operation, maintenance, service and special
application. Outside the USA, contact your local distributor for warranty information.
Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment
arrives. Printed operating manuals carefully detail equipment installation, operation and preventive maintenance.
THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL OR
IMPLIED. NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY.
Thermo shall not be liable for any indirect or consequential damages including, without limitation, damages relating to lost
profits or loss of products.
Replacement or repair of components parts or equipment under this warranty shall not extend the warranty to either the
equipment or to the component part beyond the original warranty period. The Technical Services Department must give prior
approval for return of any components or equipment. At Thermo's option, all defective parts must be returned to Thermo
postage paid and replacement parts are shipped FOB destination.
During the first year, component parts proven to be non-conforming in materials or workmanship will be repaired or replaced
at Thermo's expense, labor included. Installation and calibration are not covered by this warranty agreement. The Technical
Services Department must be contacted for warranty determination and direction prior to performance of any repairs.
Expendable items, glass, filters, gaskets, personal computers, monitors and inkjet printers are excluded from this warranty.
The Warranty Period starts two weeks from the date your equipment is shipped from our facility. This allows for shipping time
so the warranty will go into effect at approximately the same time your equipment is delivered. The warranty protection
extends to any subsequent owner during the first year warranty period.
THERMO FISHER SCIENTIFIC CONTROLLED RATE FREEZER WARRANTY
11-4
7007452 CryoMed Freezer
Thermo Scientific
Rev. 2 2/07
REGISTERED
ISO
9001
If equipment service is required, please call your Technical Services Department at 1-888-213-1790 (USA or Canada), or
1-740-373-4763. We're ready to answer your questions on equipment warranty, operation, maintenance, service and special application. Outside the USA, contact your local distributor for warranty information.
Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment
arrives. Printed instruction manuals carefully detail equipment installation, operation and preventive maintenance.
THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL OR
IMPLIED. NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY.
Thermo Forma shall not be liable for any indirect or consequential damages including, without limitation, damages relating
to lost profits or loss of products.
Replacement or repair of components parts or equipment under this warranty shall not extend the warranty to either the
equipment or to the component part beyond the original warranty period. The Technical Services Department must give prior
approval for return of any components or equipment. At Thermo's option, all defective parts must be returned to Thermo
postage paid and replacement parts are shipped FOB destination.
During the first year, component parts proven to be non-conforming in materials or workmanship will be repaired or replaced
at Thermo's expense, labor excluded. Installation and calibration are not covered by this warranty agreement. The Technical
Services Department must be contacted for warranty determination and direction prior to performance of any repairs.
Expendable items, glass, filters, gaskets, personal computers, monitors & inkjet printers are excluded from this warranty.
The Warranty Period starts two months from the date your equipment is shipped from our facility. This allows for shipping
time so the warranty will go into effect at approximately the same time your equipment is delivered. The warranty protection extends to any subsequent owner during the first year warranty period. Dealers who stock our equipment are allowed
an additional six months for delivery and installation, provided the warranty card is completed and returned to the Technical
Services Department.
THERMO FISHER SCIENTIFIC INTERNATIONAL
CONTROLLED RATE FREEZER DEALER WARRANTY
Thermo Scientific
7007452 CryoMed Freezer
AA-1
AA-2
7007452 CryoMed Freezer
Thermo Scientific
Thermo Scientific
7007452 CryoMed Freezer
AA-3
AA-4
7007452 CryoMed Freezer
Thermo Scientific
Handling Liquid Nitrogen
Warning Contact of liquid nitrogen or cold gas with the skin or eyes may cause serious
freezing (frostbite) injury. ▲
Handle liquid nitrogen carefully.
The extremely low temperature can freeze human flesh very rapidly. When spilled on a
surface, the liquid tends to cover it completely and intimately, cooling a large area. The
gas issuing from the liquid is also extremely cold. Delicate tissue, such as that of the
eyes, can be damaged by an exposure to the cold gas which would be too brief to affect
the skin of the hands or face.
Never allow any unprotected part of your body to touch objects cooled by liquid nitrogen.
Such objects may stick fast to the skin and tear the flesh when you attempt to free
yourself. Use tongs to withdraw objects immersed in the liquid, and handle the object
carefully.
Wear protective clothing.
Protect your eyes with a face shield or safety goggles (safety glasses without side shields
do not give adequate protection). Always wear gloves when handling anything that is,
or may have been, in immediate contact with liquid nitrogen. Insulated gloves are recommended, but heavy leather gloves may also be used. The gloves should fit loosely, so
that they can be thrown off quickly if liquid should splash into them. When handling
liquid in open containers, it is advisable to wear high-top shoes. Trousers (which
should be cuffless if possible) should be worn outside the shoes.
Introduction
The safe handling and use of liquid nitrogen in cryogenic refrigerators and dewar flasks is
largely a matter of knowing the potential hazards and using common-sense procedures
based on that knowledge. There are two important properties of liquid nitrogen that
present potential hazards:
1.
It is extremely cold. At atmospheric pressure, liquid nitrogen boils at -320° F
(-196°C).
2.
Very small amounts of liquid vaporize into large amounts of gas. One liter of liquid
nitrogen becomes 24.6 cu. ft. (0.7 m3) of gas.
The safety precautions in this manual must be followed to avoid potential injury or
damage which could result from these two characteristics. Do not attempt to handle
liquid nitrogen until you read and fully understand the potential hazards, their
consequences, and the related safety precautions. Keep this information handy for ready
reference and review.
Note Because argon is an inert gas whose physical properties are very similar to those of
nitrogen, the precautions and safe practices for handling and use of liquid argon are the
same as those for liquid nitrogen. ▲
Thermo Scientific
7007452 CryoMed Freezer
AA-5
Use containers designed for low temperature liquids.
Cryogenic containers are specifically designed and made of materials that can withstand
the rapid changes and extreme temperature differences encountered in working with
liquid nitrogen. Even these special containers should be filled SLOWLY to minimize
the internal stresses that occur when any material is cooled. Excessive internal stresses
can damage the container.
Do not cover or plug the entrance opening of any liquid nitrogen refrigerator or dewar.
Do not use any stopper or other device that would interfere with venting of gas.
These cryogenic liquid containers are generally designed to operate with little or no
internal pressure. Inadequate venting can result in excessive gas pressure which could
damage or burst the container. Use only the loose-fitting necktube core supplied or one
of the approved accessories for closing the necktube. Check the unit periodically to be
sure that venting is not restricted by accumulated ice or frost.
Use proper transfer equipment.
Use a phase separator or special filling funnel to prevent splashing and spilling when
transferring liquid nitrogen into or from a dewar or refrigerator. The top of the funnel
should be partly covered to reduce splashing. Use only small, easily-handled dewars for
pouring liquid. For the larger, heavier containers, use a cryogenic liquid withdrawal
device to transfer liquid from one container to another. Be sure to follow instructions
supplied with the withdrawal device. When liquid cylinders or other large storage containers are used for filling, follow the instructions supplied with those units and their
accessories.
Do not overfill containers.
Filling above the bottom of the necktube (or specified maximum level) can result in
overflow and spillage of liquid when the necktube core or cover is placed in the opening.
Never use hollow rods or tubes as dipsticks.
When a warm tube is inserted into liquid nitrogen, liquid will spout from the top of
the tube due to gasification and rapid expansion of liquid inside the tube.
Warning Nitrogen gas can cause suffocation without warning! ▲
Store and use liquid nitrogen only in a well-ventilated place.
As the liquid evaporates, the resulting gas tends to displace the normal air from the
area. In closed areas, excessive amounts of nitrogen gas reduce the concentration of
oxygen and can result in asphyxiation. Because nitrogen gas is colorless, odorless and
tasteless, it cannot be detected by the human senses and will be breathed as if it were
air. Breathing an atmosphere that contains less than 18% oxygen can cause dizziness
and quickly result in unconsciousness and death.
Note The cloudy vapor that appears when liquid nitrogen is exposed to the air is
condensed moisture; not the gas itself. The issuing gas is invisible. ▲
Never dispose of liquid nitrogen in confined areas or places where others may enter.
Disposal of liquid nitrogen should be done outdoors in a safe place. Pour the liquid
slowly on gravel or bare earth where it can evaporate without causing damage. Do not
pour the liquid on pavement.
First Aid
AA-6
7007452 CryoMed Freezer
If a person seems to become dizzy or loses consciousness while working with liquid
nitrogen, move to a well-ventilated area immediately. If breathing has stopped, apply
artificial respiration. If breathing is difficult, give oxygen. Call a physician. Keep warm
and at rest.
If exposed to liquid or cold gas, restore tissue to normal body temperature (98.6°F) as
rapidly as possible, followed by protection of the injured tissue from further damage and
infection.
Remove or loosen clothing that may constrict blood circulation to the frozen area. Call a
physician. Rapid warming of the affected part is best achieved by using water at 108°F.
Under no circumstance should the water be over 112°F, nor should the frozen part be
rubbed either before or after rewarming. The patient should neither smoke nor drink
alcohol.
Thermo Scientific
Thermo Scientific
Controlled Environment Equipment
401 Millcreek Road
Marietta, Ohio 45750
United States
www.thermo.com
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