□ This symbol denotes a step of operation being alternative to the previously described and having the same effect or leading to a follow-up step of a branching step of operation.

→ Cross-reference: Refers to another section/illustration of the manual.

1.2 Intended use of the SPECORD

The SPECORD ® is a powerful, computer-controlled double-beam spectrophotometer for the measurement of the transmission, absorbance, reflection as well as the energy of liquid and solid samples. It is designed for measurements in the spectral range 190 – 1100 nm.

The double-beam spectrophotometers of the SPECORD ® family provide different spectral resolutions:

SPECORD ® 200

SPECORD ® 205

SPECORD ® 210

1; 2 and 4 nm

1.4 nm

0.5, 1, 2 and 4 nm

The optical system without electromechanical modulator ensures true real-time operation, so that even variations in the absorbance of references can be compensated during the measurement.

The internal wavelength calibration with a holmium oxide filter provides a high wavelength accuracy and reproducibility.

The SPECORD ® complies with the regulations of the DAB (German

Pharmacopoeia) and international regulations, such as PhEur., BP, USP or JPXIII.

In combination with the powerful WinASPECT ® software, the SPECORD ® is particularly suitable for the use in routine laboratories with a high sample throughput, but also provides solutions for special applications, if used in combination with the diverse accessories available.

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SPECORD ® notes

For your personal safety and for a trouble-free operation, carefully read this chapter before starting up the SPECORD ® .

Observe all safety notes given in this manual and all messages and notes displayed by the control software on the screen.

In addition, observe the safety notes for system components of other manufacturers

(e.g. PC, printer, autosampler) supplied together with the device.

In particular, you should also observe the safety notes given on the labels and the information on the use, storage and disposal of reagents, reaction cells and the notes on the package inserts of test kits.

The SPECORD ® , including original accessories, may only be used for the applications described in this manual. The manufacturer cannot assume any liability for any other application, including that of individual modules or single parts. This also applies to all service or repair work that is not carried out by authorized service personnel. All warranty claims shall be forfeited.

Local safety regulations!

Observe the local safety regulations relevant to the operation of the device (e.g. labor-safety regulations, regulations for the prevention of and protection against accidents).

References to possible risks given in this manual do not replace the relevant laborsafety regulations that are to be followed.

Personnel!

The SPECORD ® may only be operated by appropriately qualified and trained personnel.

For that, the knowledge of the information provided by this manual is indispensable and taken for granted.

In the case of emergency, disconnect the SPECORD ® and its components from line power by removing the power plug from the power outlet.

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Safety notes SPECORD ®

The SPECORD ® is electrically powered. At several parts inside the system, extremely hazardous electrical voltages are accessible!

To ensure Protection Class I (protective earth connection) of the device, connect the power plug of the device only to a power outlet with protective earth conductor. The protective effect must not be undone by the use of an extension cable without protective earth conductor.

Before opening the device or removing any device covers, disconnect the power cable!

Before connecting the SPECORD ® to the power outlet, make sure the line voltage supplied agrees with the operating voltage specified on the rating plate at the rear panel of the device. Operation of the device with any other operating voltage than that specified may result in the destruction of the device.

Only use fuses of the specified type.

Do not operate the device in explosion-risk rooms!

Never look directly or indirectly, e.g. via a mirror, into the radiation emitted by the lamp to avoid the risk of getting conjunctivitis!

Therefore, switch off the lamp for lamp replacement!

Lamp !

Do not grasp functioning halogen and deuterium lamps on their glass bulbs.

Contamination on the bulb affects the radiation properties of the lamps.

Especially protect the beam-exit window of the deuterium lamp.

Make sure to remove the protective cap from the halogen bulb after having inserted it to avoid any risk of fire!

Non-observance will result in damage of the lamp.

Accumulation of heat

Heat accumulation might result in overheating and faults on the device.

Make sure not to cover the ventilation slots of the SPECORD ® !

Before you switch on the SPECORD ® , remove its dust cover!

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SPECORD ® notes

Take care to ensure that no liquids can enter the SPECORD ® to avoid any damage to the device.

Risk of corrosion

Do not set up the device in the vicinity of aggressive vapors, e.g. strongly corrosive acid or caustic vapors! The vapors might corrode the connections, mechanical and optical components of the device.

Avoid exposing the sample compartment to strongly corrosive substances! For the analysis of such samples, use vapor-tight stoppered cells.

After the measurement, do not leave the samples unnecessarily long in the sample compartment of the device!

The SPECORD ® was produced and tested in compliance with the following standards and regulations:

• DIN EN 61010-1 (IEC 1010-1)

• 73/23/EC

• 89/336/EC.

The SPECORD ® meets the requirements as per Protection Type IP 20.

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Safety notes SPECORD ®

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SPECORD ® Disposal

3 Disposal

The owner/operator of the SPECORD ® must ensure to dispose of the waste material

(samples) cropping up in the analyses in accordance with the relevant legal and local regulations.

When the service life of the device is over, dispose of the device with its electronic components as electronic scrap observing the relevant regulations.

You can also return the SPECORD ® to Analytik Jena AG for disposal. Please contact our Technical Service.

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Disposal SPECORD ®

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SPECORD ® Technical data of the spectrophotometers

Optical data of SPECORD®

4 Technical data of the spectrophotometers

4.1 Optical data of SPECORD

Principle

Optics

Light sources

SPECORD

200 / 205 / 210

Double-beam spectrophotometer with two largearea Peltier temperature-controlled photodiodes

Monochromator with holographic concave grating

Light split into two beams by a beam-splitting plate

Deuterium lamp and halogen lamp, pre-aligned

Wavelength range

Photometric range

Wavelength accuracy

Wavelength reproducibility

Stray light

Photometric accuracy VIS

Photometric accuracy UV

Photometric reproducibility

Long term stability

Baseline stability

Zero-line of transmission

RMS noise

190 ... 1100 nm

- 3 ... + 3 A

± 0.5 nm at 360,9 nm with holmium glass filter

± 0.1 nm (5 measurements)

< 0.5 % T at 200 nm (KCl)

< 0.05 % T at 220 nm (NaJ, ASTM) *

< 0.05 % T at 340 nm (NaNO

, ASTM)

± 0.005 A at A = 1

± 0.005 A at A = 1; potassium dichromate

± 0.0005 A/h at A = 1

± 0.001 A/h at 500 nm (1 h warm-up)

± 0.001 A from 200 to 800 nm with slit 2 nm

± 0.03 %T

± 0.0001

Scanning speed 30, 60, 120, 300, 600, 1200, 3000, 6000 nm/min

*)allowing for tolerances of used standard

Spectral bandwith

Toluene / hexan resolution

SPECORD

200 SPECORD

1 / 2 / 4 nm, variable 1.4 nm

205 SPECORD

0.5 / 1 / 2 / 4 nm, variable

210

> 2.1 > 1.7 > 2.1

Overview 4-1 Technical data of SPECORD ® 200

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Technical data of the spectrophotometers

General technical data of SPECORD®

SPECORD ®

4.2 General technical data of SPECORD

Weight

Footprint (W x D)

Dimensions (W x H x D)

Line voltage

Line frequency

Power consumption

Fuses:

Line fuses

Radio noise suppression

Noise immunity

Protection Type

Data interface

PC requirements for software:

Aspect Plus and WinASPECT

Overview 4-2 General technical data of SPECORD ®

18 kg approx. 1200 x 750 mm 2 with PC

680 x 240 x 620 mm 3

230 VAC ±10 % and switchable to

115 VAC ±10 % (by Technical Service)

47 ... 63 Hz

195 VA

230 VAC: T 3.15 AH/250V, Type 19181

(Wickmann)

115 VAC: T 3,15 AH/250V, Type19181

(Wickmann) as per EN 55011 Class A as per DIN EN 50082-1

IP 20

RS-232 port

MS Windows 2000 operating system or higher

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SPECORD ® Device description and operating principle

Mechanical design of SPECORD®

5 Device description and operating principle

5.1 Mechanical design of SPECORD

Fig. 5-1 Mechanical design of SPECORD ®

2 Desiccator

3 Cover of spectrometer system and photometer section 8

Device control

Power supply module

The major subassemblies of the SPECORD ® are:

Light sources

(1)

Spectrometer system

Support plate

Holder for cells

Sample compartment

Halogen lamp and deuterium lamp generate the light that is monochromatized in the spectrometer system. Then, it passes through the sample.

The spectrometer system is protected against extraneous light and dust by cover

(3)

. The desiccator

(2) regulates the humidity inside the system.

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Device description and operating principle

Mechanical design of SPECORD®

SPECORD

Photometer section This subassembly serves to split the light exiting the monochromator into sample and reference beam. The optical system shapes the beam cross-section in the sample compartment. The photometer section, too, is protected by cover

(3)

Sample compartment

(10)

The sample compartment contains two pairs of carrier rods for the accommodation of various accessory units.

To the support plate

(8)

, two holders for the accommodation of cells

(9)

and the detector board are mounted.

Detector board

(6)

Two photodiodes are used as radiation detectors, which on the SPECORD ® 200/210 are Peltier temperaturecontrolled.

Power supply module

(5)

Device control computer

(4)

The power supply modules supply the electrical voltage to the electronic system and the fans for the thermoelectric temperature control of the detectors on the SPECORD ® 200/210.

The device control computer coordinates the interaction of the subassemblies involved in the measurement processes.

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SPECORD ®

5.2 Design of subassemblies

Device description and operating principle

Design of subassemblies

Fig. 5-2 Lamp compartment (without cover)

1 Halogen lamp in protective housing

2 D

E lamp compartment

Toric illuminating mirror

Setscrew for mirror adjustment

The light sources used are a deuterium lamp

(2)

for the UV region and a halogen lamp

(1)

for the VIS region of the spectrum.

Depending on the position of reflecting mirror

(3)

, which is mounted onto a moving magnet, the radiation of either deuterium or halogen lamp is sent onto the entrance slit of the monochromator. You can choose the wavelength at which the lamp is changed over a wide range using the WinASPECT ® control and analysis software.

The toric mirror

(4)

serves for imaging the light sources onto the entrance slit of the monochromator. To obtain the maximum possible energy of the deuterium lamp for the measurement, you can sensitively adjust the setscrew for mirror adjustment

(5)

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Device description and operating principle

Design of subassemblies

SPECORD

5.2.2 Spectrometer system and photometer section

SPECORD 200 – Optical Diagram

Concave grating

Entrance slit

Exit slit

Plane mirror

Filter wheel

Reference cell

Beam splitter

Sample compartment

Sample cell Halogen lamp

Swivel mirror

Quartz-coated toric mirrors

Cavity for turbid samples

Detector

Fig. 5-3 Schematic setup – Optical diagram of SPECORD ®

The spectrometer system with the optical elements illustrated above – filter wheel, concave grating and slit assembly – works as monochromator.

The filter wheel contains the following filters:

4 color glass filters

Holmium filter

2 blank positions

Used to suppress unwanted radiation in the monochromator.

Standard for the automatic recalibration of wavelengths.

Block position Needed for the generation of measured data.

The entrance and exit slits of the spectrometer system are mounted to the slit carrier. Depending on the device model, the slits allow the following spectral slit widths:

SPECORD ® 200

SPECORD ® 205

SPECORD ® 210

1, 2, 4 nm

1.4 nm

0.5, 1, 2, 4 nm.

The imaging grating disperses the incident light and spreads it out into a spectrum.

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SPECORD ® Device description and operating principle

Design of subassemblies

Computer-controlled stepper motors drive the filter wheel, the slit carrier and the linear actuator for moving the grating. Because of the little number of moving parts used in the spectrometer system, the SPECORD ® ensures a high reliability of its optical parameters.

In the photometer section, the mirror system divides the beam coming from the monochromator into sample and reference beam. The imaging mirrors shape the beam to the cross-sections needed in the sample compartment.

Spectrometer system and photometer section are protected against dust and reagents by a cover. However, corrosive reagents or strongly evaporating liquids should be inserted in the sample compartment only in stoppered cells.

Fig. 5-4 Sample compartment

Sample beam path with 2 carrier rods

Reference beam path with 2 carrier rods

Connector for sipper and cell changer

Reserve connector

Stopper for optical fiber of measuring probe

Conduits for coolant hoses

Mount for cell holder

Receptacle for cells with turbid samples

Mount for cell holder

Conduit for sample drain hose

Conduits for sample-aspirating hoses (sipper)

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Device description and operating principle

Design of subassemblies

SPECORD ®

Sample

(1)

and reference beam

(2)

enter the sample compartment through the lefthand wall.

The mounts for the cell holders

(7)

(8)

are arranged in front of the exit windows in the right-hand sample compartment wall.

The two pairs of carrier rods serve to accommodate the universal holder and other accessories, which easily clamp to the rods.

The receptacles for the cells

(8)

with turbid samples are located directly in front of the detector at the right-hand sample-compartment wall.

Two 9-pin connectors

(3), (4)

allow the connection of electrically driven accessories, such as the automatic temperature-controlled cell changer or the sipper system.

The optical fibers of the measuring probe or the cables of the Peltier temperaturecontrolled cell holder or the cell changer are threaded through the recess in the rear wall of the sample compartment

(5)

The three conduits

(6,11)

in the right-hand sample compartment wall are used for threading through the sample drain hose and two coolant hoses for the thermostat.

Two openings in the front of the device cover are provided for the aspiration tubes of the super sipper system.

The illustration below shows the geometrical dimensions of the sample compartment.

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SPECORD ®

193

Device description and operating principle

Design of subassemblies

Fig. 5-5 Dimensions of sample compartment

1 Front side of sample compartment

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Device description and operating principle

Design of subassemblies

SPECORD ®

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SPECORD ® requirements

Considering the dimensions of the SPECORD ® and a clearance of approximately

100 mm to the sides and the front, the device needs a bench space of at least

860 x 720 mm.

660

Fig. 6-1 Dimensions of SPECORD ®

520

Caution!

Do not cover the ventilation slots of the device!

Do not place any objects on the device!

Do not operate the device in explosion-risk rooms!

Keep aggressive vapors, e.g. strongly corrosive acid or caustic vapors away from the vicinity of the device.

The installation site of the SPECORD ® must meet the following requirements:

• The place should be free of draft, dust, corrosive vapors as well as vibrations.

• Do not set up the SPECORD ® near electromagnetic fields (e.g. of motors).

• Avoid dripping or splashing water near the instrument.

• Do not expose the device to direct sunlight or the radiation of heaters.

• Temperature range: + 15 ... + 35 °C

• Humidity: up to 90 % (at + 30 °C)

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Installation requirements SPECORD ®

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SPECORD ® and

Connectors and display elements

7 Installation and start-up

7.1 Connectors and display elements

The connectors and display elements of the SPECORD ® are conveniently located on the front and the side panels of the SPECORD ® .

The LEDs indicating Power ON and lamp standby are located on the left side of the front panel of the SPECORD ® .

1 2 3

Fig. 7-1 Display elements on front panel

1 Power ON indicator LED (green)

2 D

E lamp ON indicator LED (yellow)

3 Halogen lamp ON indicator LED (red)

The LEDs light when the SPECORD ® has been switched on and the lamps are on.

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Installation and start-up

Connectors and display elements

SPECORD ®

The line power input connector with fuse holder and the power switch are located on the left-hand side panel of the device.

4 3 2 1

Fig. 7-2 Connectors on left-hand side panel

Power switch 3

Power input connector 4

Fuse holder for line fuse

Nameplate

On the right-hand side panel of the device, the following connectors are located:

RS 232 and USB ports for the connection of a PC and the connectors for the xyz autosampler and the control of the Peltier temperature-controlled cell holder.

Page 24

4 3 2 1

Fig. 7-3 Connectors on right-hand side panel

RS 232 port for PC

USB port for PC

Connector for xyz autosampler

Connector for control of Peltier temperature-controlled cell holder

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SPECORD ® and

Removing the transport lock

7.2 Removing the transport lock

The SPECORD ® is equipped with a transport lock to prevent damage to the device and its optical system during transportation. The lock screw is located at the rear right on the bottom part of the device.

Transport lock

Fig. 7-4 Transport lock on SPECORD ®

1 Transport screw

Note

The transport lock screw is not removed from the device, but is left in its holder in the SPECORD ® .

If the device must be transported again or returned for repair to Analytik Jena AG, lock the transport lock again.

Releasing the transport lock

Using a screwdriver turn the transport lock screw

(1)

anti-clockwise as far as it will go.

Locking the transport lock

First, initialize the device to bring the monochromator into its starting position.

Switch the device off.

Using a screwdriver, turn the transport lock screw

(1)

clockwise as far as it will go.

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Installation and start-up

Connecting the SPECORD®

SPECORD ®

7.3 Connecting the SPECORD

Observe correct position of device!

Move the SPECORD ® only in upright position (observe label on transport case).

Do not turn the device upside down.

Loosen the transport lock screw located on the rear left of the SPECORD ® .

Electric shock

Before connecting the SPECORD ® and the PC to line power, make sure the available line voltage agrees with the operating voltage specified on the type labels.

Operation of the devices on line voltages other than the specified operating voltage may result in their destruction.

Standard equipment

The standard equipment of the SPECORD ® includes the following items:

• 2 cell holders

• Installation CD-ROM of WinASPECT ®

• Spare halogen lamp

basic module

• Deuterium lamp

• Power cable

• PC – SPECORD ® connecting cable

• Spare fuses 5 x 20 T 3.15 A L

• User's Manuals for the SPECORD ® , the accessories and the

WinASPECT ® control and analysis software.

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SPECORD ® Installation

Connecting the SPECORD®

Connecting SPECORD ® and PC

Remove the device from its transport packaging and remove the plastic cover.

Wait until the device has adjusted to the ambient temperature.

Remove the foamed plastic packaging from the SPECORD ® sample compartment. The packaging contains the items included in the standard equipment (see above) and the installation disks for the WinASPECT ® optional module you ordered.

Install the software on the PC ( → Refer to WinASPECT ® User's Manual).

Insert the deuterium lamp following the instructions given in Section

9.3 Changing lamps p. 36 .

Connect the SPECORD ® ( → see

7.1 Connectors and display elements p. 23 ) to

the PC (free COM port) using the supplied connecting cable.

Connect one power cable each to the SPECORD ® and the PC. Connect the power cables of the SPECORD ® and the PC to power outlets that are connected to the same phase of the line power supply (multiple socket outlet, if possible).

Note

When required, align the lamps following the instructions given in Section

9.3

Changing lamps, p. 36 after start-up.

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Installation and start-up

Start-up

SPECORD ®

7.4 Start-up

Do not cover ventilation slots of the device!

Take care to ensure that the ventilation slots on the top and on the right and left bottom of the SPECORD ® are not covered. Do not place any objects in front of the openings. The suction intakes for the two fans on the bottom of the device must be kept free.

Caution!

Do not place any objects, especially no containers with liquids, on the SPECORD ® .

Start WinASPECT ® software by a click on the program icon

Windows desktop or by clicking the Start button on the taskbar:

< Start I Programs I WinASPECT I WinASPECT > .

on the

Using the power switch on the left panel of the device, switch the SPECORD ® on ( →

Refer to Fig. 7-2 Connectors on left-hand side panel, p. 24 ).

The green Power ON LED on the front panel of the device lights.

( →

Refer to Fig. 7-1 Display elements on front panel, p. 23 ).

In WinASPECT ® software, select the COM port for the SPECORD ® – PC connection:

Activate the menu command < Measurement I Set Parameters > to open the window of the device driver SPECORD xxx ...

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SPECORD ® and

Start-up

Fig. 7-5 Dialog box of SPECORD ® 200 device driver

Click on the Device tab.

From the COM Port list box, select the COM port of your PC, to which the

SPECORD ® is connected.

Click on [OK] to close the device driver window.

Note

The selected COM port will be saved and remain set until you change the COM port setting. Thus, you need not select it after every restart of the device.

Initialize the device:

Establish communication between SPECORD ® and PC using the menu command < Measurement I Initialize Device > .

The SPECORD ® is now ready for taking measurements.

Note

Please note that the SPECORD ® reaches thermal stability only after a warm-up time of one hour. Therefore, you should not start precision measurements unless the device has warmed up.

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Installation and start-up

Switching the device off

SPECORD ®

7.5 Switching the device off

7.5.1 Switching the deuterium lamp off

If you have worked with the deuterium lamp and you are sure that you do not need it any longer this day, you can switch it off by software control following this procedure:

In WinASPECT ® , activate the device driver with menu command

< Measurement I Set Parameters > .

Activate the Device tab.

In the Lamp change at field, deactivate the D

E automatically function.

Close the device driver window by a click on [OK] .

Reinitialize the device using the following menu command: < Measurement I

Initialize Device > .

A query appears on the screen asking you if you want to switch on the UV lamp.

Click the [No] button.

The UV lamp goes out.

7.5.2 Switching the SPECORD ® off

We recommend that the SPECORD ® be powered down by software control after you have finished taking measurements:

Exit WinASPECT ® using menu command < File I Exit > .

Then, shut down the computer.

Finally, switch the SPECORD ® off with the power switch.

Note

If you have finished the measurements on the SPECORD ® but want to analyze the obtained results on the PC, you can also switch off the SPECORD ® without shutting down the computer before.

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SPECORD ® Accessories

Standard cell holders

8 Accessories

8.1 Standard cell holders

The standard equipment of the SPECORD ® includes two cell holders for cells of up to 50 mm pathlength.

The mount for the cell holder is located on the right-hand sample compartment wall in front of the beam-exit window ( →

Fig. 5-4 Sample compartment, p. 17 ). The cell

holder is designed for cells having a pathlength of up to 50 mm and a width of

12.5 mm.

Fig. 8-1 Standard cell holder

Cell support

Guide

Push the cell holder with its guide

(2)

from top onto the cell-holder mount.

The beam transmits the cells at a height of approximately 5 to 15 mm above the support of the cell holder.

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Accessories

Receptacle for cells with turbid samples

SPECORD ®

Inserting the cell

Fill the sample into the cell up to a level of at least 20 mm.

Insert the cell so it rests against the right-hand support surface of the cell holder.

Correct

Fig. 8-2 Inserting the cell

Note

In absorbance measurements, the pathlength is one of the crucial factors.

Therefore, it is important to insert all samples (samples and references) in the same location and position. In this way, you can avoid measurement errors caused by angular differences and parallel offset of the beam.

8.2 Receptacle for cells with turbid samples

In the measurement of strongly turbid samples, a considerable portion of the radiation does not exit the sample cell straightly, but is scattered within a solid angle.

For photometrically accurate measurements it is necessary that the detector capture the total scattered radiation. For this purpose, the sample and the reference substance must be arranged as close to the detector as possible.

Therefore, the receptacles for the cells with scattering samples are located in the right-hand sample-compartment wall of the SPECORD ® in front of the photodiode detectors (

Fig. 5-4 (8) p.17

). The receptacles can accommodate standard cells of

10 mm pathlength.

Remove the foamed-plastic caps covering the cell receptacles and insert the cells for the analysis.

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SPECORD ® Accessories

After the measurement is finished, make sure to cover the cell receptacles again with the foamed-plastic caps. If you fail to do so, extraneous light might be incident on the detector and distort the results of the measurements.

Optionally the following accessories are available for the SPECORD :

• Universal cell holder

• Thermostatted cell holder, water-cooled

• Thermostatted cell holder; Peltier-controlled

• Adjustable cell holder for micro, submicro or HPLC cells

• 6-cell changer, water-cooled

• 6-cell changer, Peltier-controlled

• Holder for cylindrical cells of up to 100 mm

• Holder for round cells

• Holder for solid samples

• 11° ... 60° variable Angle Reflectance Accessory

• Absolute Reflectance Accessory

• Universal sipper system with flow cell and peristaltic pump

• XYZ autosampler

• Fiber coupling with measuring probes

• 75-mm integrating sphere for transmission and diffuse reflectance

• PTC 100, Peltier temperature-controlled cell holder

• PTC 600, Peltier temperature-controlled 6-cell changer

• SMA fiber connector

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Accessories

Additional accessories

SPECORD ®

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SPECORD ®

9 Care, maintenance, lamp change

Care, maintenance, lamp change

Care and maintenance

The SPECORD is largely free of maintenance.

To ensure your laboratory certification with device validation, Analytik Jena AG offers you a service/maintenance agreement.

User care and maintenance is limited to the following operations:

• Cleaning of sample compartment and device casing

• Lamp change

• Change of line fuses

• Change of desiccator

You should only use spares from Analytik Jena AG .

You can order consumables and wear parts by phone from our Service Department:

Hotline No.: + 49 3641-77-7401

In the case of malfunction of the device, please contact our Service Department. For the address, refer to the → inside cover page .

9.2 Cleaning

Avoid keeping filled cells unnecessarily long in the sample compartment to prevent the spectrometer from being affected by any solvent vapors.

Wipe off spilt samples in the sample compartment or on accessory units instantly with cleaning tissue.

Wipe off contamination on the instrument with a soft, clean cloth slightly moistened with a commercially available, neutral detergent.

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Analytik Jena AG

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Care, maintenance, lamp change

Changing lamps

SPECORD

9.3.1 Note on the service life of lamps

The service life of lamps is:

• Halogen lamp: > 500 h (at 5.0 V operating voltage)

• Deuterium lamp: > 1000 h

The service life of the lamps is reduced, if the SPECORD is frequently switched on and off. This particularly applies to the deuterium lamp, because of the relatively high ignition voltage required.

The following rule applies as criterion for the service life of the deuterium lamp:

With one daily ON/OFF switching operation, its radiation intensity is allowed to decrease to 50% after 1000 hours.

The deuterium lamp must be changed, if its heating coil has burnt out, its energy is insufficient or the operating hours counter indicates 2000 h.

9.3.2 Opening the flap of the lamp compartment

The flap of the lamp compartment (front left corner of device cover) is to be opened to check the operating hours of the deuterium lamp, change the lamps or change the desiccator.

Switch the SPECORD ® off and allow the lamps to cool down for at least five minutes.

Release the lock

( 2 )

of the flap of the lamp compartment by folding the lock frontward so it sticks out from the SPECORD ® approximately vertically.

Remove the flap of the lamp compartment by lifting it from below by 1 to 2 mm and then pulling it off to the left.

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SPECORD ® Care, maintenance, lamp change

2 1

Opening the flap of the lamp compartment

Flap of lamp compartment

Lock

Fig. 9-1

In the open lamp compartment, you can see the deuterium lamp at the front left and the halogen lamp. Both lamps are enclosed by a protective cover.

4 3 2

Fig. 9-2 Open lamp compartment

Protective cover of halogen lamp

Knurled screw of halogen lamp sleeve

Protective cover of deuterium lamp

Top part of deuterium lamp cover

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200/205/210 User's Manual – Edition 03/2003

Analytik Jena AG

Page 37

Care, maintenance, lamp change

Changing lamps

SPECORD ®

9.3.3 Changing the deuterium lamp

Risk of electric shock!

Before changing the deuterium lamp, make sure to switch the device off with the power switch.

Caution: Risk of burns!

Allow the deuterium lamp to cool down to avoid the risk of burns!

Emitted UV radiation!

Never look directly or indirectly, e.g. via a mirror, into the radiation emitted by the lamp to avoid the risk of getting conjunctivitis!

Dirt on beam exit window!

Do not touch the glass bulb of the new deuterium lamp. Particularly avoid contamination on the quartz glass beam exit window! If you touched the glass bulb with your fingers, wipe the fingerprints off with a clean, lintfree cloth and pure alcohol. The performance of the lamp will deteriorate by contamination.

Before you can change the lamp, remove the top part of the protective cover (

Fig. 9-2

(4)

) by pulling it upward.

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SPECORD ® Care, maintenance, lamp change

6 7 8

2 1 12 11

Fig. 9-3 Lamp protective cover partly removed

1 Holder for halogen lamp

2 Knurled screws

3 Halogen lamp

4 Deuterium lamp

5 Connector

6 Spring-loaded straps

Operating hours counter

Quick-lock nuts

Guiding groove

Illuminating mirror

Setscrew

Fastening screws for halogen lamp holder

Disconnect the three-pin connector

(5)

by compressing the lateral straps

(6) and taking the connector off. Release the quick-lock nuts

(8)

and pull out the defective deuterium lamp

(4)

Remove the new deuterium lamp from its packaging and insert it in the lamp holder taking care that the locating pin of the lamp holder fits into groove

(9)

of the lamp base.

Screw down the deuterium lamp by means of both quick-lock nuts.

Fasten the connector to the lamp.

Finally, reattach the top part of the protective cover.

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200/205/210 User's Manual – Edition 03/2003

Analytik Jena AG

Page 39

Care, maintenance, lamp change

Changing lamps

SPECORD ®

Fine alignment of deuterium lamp

Despite stringent manufacturing tolerances of the deuterium lamps, it may be necessary to realign the lamp after lamp change to ensure that maximum intensity of the lamp is incident on the entrance slit of the monochromator. For this purpose, you can slightly move the illuminating error

(10)

by means of setscrew

(11)

Switch the SPECORD ® on and initialize it.

To this end, start WinASPECT ® and activate the following menu command:

<Measurement I Initialize Device> .

Set up a parameter record containing the following parameters:

Integration time 0.5 s

Display Energy

For the alignment, the protective cover of the halogen lamp must be kept in place.

Start the measurement with the sample compartment kept closed. Keep the flap of the lamp compartment open.

Sensitively turn the setscrew

(11)

in both directions until the detected energy reaches its maximum.

This adjustment also influences the alignment of the halogen lamp. However, the variation is comparatively small and normally negligible.

Finally, close the flap of the lamp compartment.

Note

Please note that the deuterium lamp needs a warm-up of one hour after ignition to reach full thermal stability.

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SPECORD ® Care, maintenance, lamp change

9.3.4 Changing the halogen lamp

Before changing the halogen lamp, make sure to switch the device off with the power switch.

Caution: Risk of burns!

Allow the halogen lamp to cool down to avoid the risk of burns!

Warning!

Never look into the lighting lamp as the radiation may cause eye injury!

Caution!

Do not touch the glass bulb of the lamp to avoid any contamination affecting its performance!

After having opened the flap of the lamp compartment, you can see the protective cover of the halogen lamp.

Loosen the knurled screw (

Fig. 9-2

(2)

) and remove the protective cover by lifting it off.

Loosen the two clamping screws (

Fig. 9-3

(2)

Pull the defective halogen lamp (

Fig. 9-3

(3)

) out of its holder

(1)

Remove the new halogen lamp from its packaging, but keep the protective sheath attached.

Insert the new halogen lamp taking care to ensure that the notch at its base encloses the pin in the bottom part of the holder.

Caution!

Make sure to remove the protective sheath of the halogen lamp now! Failure to do so involves the risk of fire and lamp damage. Do not touch the glass bulb of the new halogen lamp! Any contamination will impair the lamp performance.

Reattach the protective cover of the halogen lamp and refasten it.

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Analytik Jena AG

Page 41

Care, maintenance, lamp change

Changing lamps

SPECORD ®

Fine alignment of halogen lamp

Check the alignment of the halogen lamp.

For this purpose, switch the SPECORD ® on and initialize it.

To this end, start WinASPECT ® and activate the following menu command:

< Measurement I Initialize Device > .

Exit WinASPECT ® to run the Service Check routine.

Activate the Service Check routine as follows:

Click on the Start button of the Windows taskbar. In the Programs folder, click on the WinASPECT folder. There, click on the Service xxx command. This will bring up the SPECORD xxx - Service Check dialog box.

Click on the Zeroth Order button and choose the halogen lamp ( HL – Lamp ).

View the image of the sample beam in the sample compartment by holding a white sheet of paper in the plane where normally the cell is placed.

There, a 7 – 8 mm high and 1 – 2 mm wide light spot should be visible that is evenly illuminated from top to bottom.

If you find that the light spot on the sheet of paper is badly uneven, verify that the halogen lamp is correctly seated.

If you are not satisfied with the alignment of the halogen lamp, loosen the fastening screws (

Fig. 9-3, (12)

) and move the lamp sensitively while running an energy measurement as described above for the fine alignment of the deuterium lamp. In this case, the ideal wavelength is 500 nm.

Note

Please note that the halogen lamp needs a warm-up of about one hour after switch on to reach full thermal stability.

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SPECORD ®

9.4 Changing the desiccator

Care, maintenance, lamp change

Changing the desiccator

Before changing the desiccator, switch the SPECORD ® off with the power switch and disconnect the power cable from the power input connector of the SPECORD ® .

The desiccator protects the optical system of the SPECORD ® against too high air humidity. The desiccator is accessible, when the lamp compartment has been opened (

→

Refer to

Fig. 5-1 p. 13 ).

Especially in tropical countries, the adsorption capacity of the desiccator should be checked regularly. The adsorption capacity of the desiccator is exhausted, if the color of the indicator paper in the inspection window has changed from blue to pinkyellow.

For changing the desiccator, follow this procedure:

Open the flap of the lamp compartment by folding the lock frontward so it sticks out vertically. Lift the flap from below by 1 to 2 mm and then pull it off to the left.

Using a flat tool, such as an appropriate coin, unscrew the desiccator from its holder.

Remove the new desiccator from the packaging and screw it into the holder.

Finally, close the flap of the lamp compartment.

Used up desiccators can be reused after heating them up for five hours in a drying oven. New desiccators are available from our Service Department ( → for the address, refer to the inside cover page).

SPECORD

200/205/210 User's Manual – Edition 03/2003

Analytik Jena AG

Page 43

Care, maintenance, lamp change

Changing fuses

SPECORD ®

If the line fuses are defective, you can change them yourself.

Risk of electric shock!

Before changing the fuses, make sure to switch the SPECORD ® off with the power switch and disconnect the power cable from the power input connector of the device.

Remove the power cable from the SPECORD ® .

Open the fuse holder (