Imaging Systems, Software, and Accessories

Imaging Systems, Software, and Accessories
PAGE 372 GEH Ch11ToC FINAL.pdf
09/06/2008
12:54:29
Gel and Blot Imaging
& Analysis
2005, Dr. Ahmet Yildiz
Elucidating the mechanism of molecular
motor movement
Dr. Yildiz received a bachelor's degree in physics from Bogazici
University, Istanbul, and started his graduate studies in
biophysics at the University of Illinois Urbana-Champaign.
C
M
Working in the research group of Dr. Paul Selvin, he developed
the technique of fluorescence imaging with one-nanometer
accuracy (FIONA). This work was recognized with a Foresight
Institute Distinguished Student Award in 2003.
Y
CM
MY
CY
CMY
He went on to use FIONA to study the molecular walking
mechanism of the motor proteins myosin V, myosin VI, and
kinesin. Dr. Yildiz received his PhD in 2004, and his thesis
was awarded the Gregorio Weber International Prize in
Biological Fluorescence.
K
GE & Science Prize for Young Life Scientists is
supported by GE Healthcare and the journal Science,
which is published by AAAS, the nonprofit society.
Used with permission of AAAS © 2007.
Further information on how to enter, plus past
winners and their essays can be found at:
www.gelifesciences.com/science
ch10.fm Page 374 Wednesday, June 18, 2008 4:26 PM
Imaging Systems, Software, and Accessories
Typhoon imagers are our high-end variable mode imagers
encompassing phosphor and fl uorescence applications.
Typhoon imagers deliver outstanding linearity, quantitative
accuracy, and extremely low limits of detection. Automated
multicolor scanning permits detection of multiple samples
in the same experiment. This ensures accuracy of analysis,
increases throughput, and saves time.
PAGE: 374 - CURRENCY: USD
Storm Gel and Blot Imaging Systems provide proven quality and
high-performance for filmless autoradiography and specific fluor
applications. The systems have wide exposure range and accurate
signal quantitation that yield publication quality images on the
first exposure. Storm phosphorimagers use storage phosphor
screens instead of film to deliver high-resolution phosphorimages
and accurate quantitation of various sources of ionizing radiation.
PAGE: 374 - CURRENCY: USD
Ettan DIGE Imager is a high-quality imager for a wide range of
fluorescence gel and blot applications. In particular it is validated
for use with Cy2-, Cy3- and Cy5-labeled 2-D DIGE gels and
Amersham ECL Plex quantitative Western blotting applications.
10
PAGE: 374 - CURRENCY: USD
ImageQuant CCD-based imaging systems cover the full range
of gel documentation, chemiluminescence, and fluorescence
applications. CCD imaging creates digital data for archiving,
publication, and, with some models, quantitative analysis.
For some users, a CCD imager is an appropriate partner or
replacement to film–based chemiluminescence applications.
ImageScanner III is a versatile densitometer with high linearity
that is designed for accurate and sensitive densitometric
quantitation of SDS-PAGE gels, blots, membranes, and slides.
374
For more information, visit www.gelifesciences.com
PAGE: 374 - CURRENCY: USD
Gel and Blot Imaging & Analysis
SELECTION GUIDE – Imaging Systems
GE Healthcare
ch10.fm Page 375 Wednesday, June 18, 2008 4:26 PM
Imaging Systems, Software, and Accessories
SELECTION GUIDE – Typhoon, Storm, and ImageQuant Imager Performance
Typhoon Typhoon Typhoon Typhoon Typhoon Typhoon Storm
Trio+
Trio
9410
9400
9210
9200
860
PAGE: 375 - CURRENCY: USD
PAGE: 375 - CURRENCY: USD
PAGE: 375 - CURRENCY: USD
Fluorescence—Proteomics
CyDye DIGE Fluors
Cy2
Cy3
Cy5
ECL Plex Fluors
Cy2
Cy3
Cy5
Protein Stains
Deep Purple Total Protein Stain
SYPRO Ruby
NanoOrange (solutions)
Pro-Q Diamond (phosphorylated proteins)
Pro-Q Sapphire 532 (Histidine-tagged
proteins)
Storm
820
ImageQuant ImageQuant
RT ECL
350
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Fluorescence—Genomics
Microarray
Cy3 and Cy5
Alexa Fluor 532 and Alexa Fluor 633
Nucleic Acid Stains
Ethidium Bromide (post stain)
Vistra Green, SYBR Gold, SYBR Green I & II
PicoGreen, RiboGreen
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Chemifluorescence (enzyme catalyzed)
ECL Plus Western Blotting
ECF, AlkPhos Direct ECF
DDAO Phosphate
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Multipurpose Fluorescence
Cy2
Cy3
Cy5
Fluorescein, FAM, FITC, Alexa Fluor 488
TET, HEX, ROX, TAMRA
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Other Applications
GFP
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Chemiluminescence
ECL
ECL Plus
ECL Advance
Gel and Blot Imaging & Analysis
PAGE: 375 - CURRENCY: USD
Storage Phosphor
32P, 125 I, 14C, 33P, 35 S, 3 H
Macroarray (radiolabeled)
Storm
840
10
++++ = superior performance
+++ = high performance
++ = good performance
+ = acceptable performance
– = not compatible
Ratings are based on overall system performance including model-specific features, versatility, and sensitivity (limit of detection)
GE Healthcare
For more information, visit www.gelifesciences.com
375
ch10.fm Page 376 Wednesday, June 18, 2008 4:26 PM
Imaging Systems, Software, and Accessories
Typhoon Variable Mode Imager
ORDERING INFORMATION
Typhoon Trio
Includes: ImageQuant TL†
1 63-0055-87
Typhoon Trio+
1 63-0055-89
Includes: ImageQuant TL†
Typhoon 9400
1 63-0055-78
Includes: ImageQuant TL†
Typhoon 9410
1 63-0055-80
Includes: ImageQuant TL†
Workstation scanner with monitor
1 28-4086-78
1 63-0028-90
1 63-0028-92
Low-fluorescence Glass Plates, 27 u 21 cm, for Ettan DALT
(including spacers)
1 80-6475-58
Low-fluorescence Glass Plates for SE600, 18 u 16 cm
Spacers for SE 600/SE 400 Vertical Gel Units
2 cm u 16 cm u 1.00 mm (W u L u T)
2 80-6442-14
2 80-6180-70
Spacers for SE 600/SE 400 Vertical Gel Units
1 cm u 16 cm u 1.00 mm (W u L u T)
2 80-6179-94
Kapton Tape Roll 3 mm u 33 m
Wonder Wedge plate separation tool
1 63-0028-94
1 80-6127-88
Microarray Slide Holder*
Image Eraser
1 63-0039-99
1 63-0007-83
Ettan DIGE Gel Alignment Guides for Ettan DALT
Ettan DIGE Gel Alignment Guides for SE 600
1 80-6496-10
1 80-6496-29
Typhoon Multislide Tray
1 63-0054-34
For pricing information, visit www.gelifesciences.com/orderonline
* For Typhoon 9210 and Typhoon 9410 models only
† Requires approved computer
Related Products
Refer To
page 381
page 382
ImageQuant TL
Amersham ECL Plex Western Blotting Combination Packs
page 388
page 354
Amersham ECL Plex CyDye-conjugated Antibodies
CyDye DIGE Fluors
page 354
page 264
Ettan DIGE Imager
page 383
DeCyder 2-D Differential Analysis Software v6.5
DeCyder Extended Data Analysis (EDA) Software v1.0
page 265
page 266
ImageMaster 2D Platinum v6.0
Ettan DALTtwelve Large Vertical System
page 268
page 277
Ettan DALTsix Large Vertical System
Multiphor II Electrophoresis System
page 274
page 291
Ettan IPGphor 3 IEF System
page 272
Typhoon 9410 Variable Mode Imager unites proven storage
phosphor autoradiography technology with four-color,
nonradioactive fluorescent labeling techniques. For DNA, RNA,
and protein samples, choose from:
Storage phosphor autoradiography
Direct blue-excited fluorescence (457, 488 nm)
Direct green-excited fluorescence (532 nm)
Direct red-excited fluorescence (633 nm)
Chemiluminescence
For more information, visit www.gelifesciences.com
GE Healthcare
PAGE: 376 - CURRENCY: USD
Storage Phosphor Screens and Cassettes
Image Eraser
J
J
J
J
J
376
1 63-0055-76
PAGE: 376 - CURRENCY: USD
Four-color competitive gel shift assay to determine the relative affinities of the
Salmonella spp. bacteriophage P22 repressor Mnt (1). In the assay the repressor is
allowed to bind four different oligonucleotides labeled with FAM, HEX, TAMRA, and
ROX respectively at the same time. The intensities for each fluorophore in a lane
are related directly to the relative affinity of the repressor protein for the
oligonucleotide used.
Typhoon 9210
Includes: ImageQuant TL†
PAGE: 376 - CURRENCY: USD
10
J Versatile system platform, handles gel sandwiches,
agarose and polyacrylamide gels, membranes,
microplates, and even microarrays.
J Powerful excitation sources and innovative high-quality
confocal optics allow for the sensitive detection of
low-abundance targets.
J Red-, green-, and blue-excitation wavelengths and a wide
choice of emission filters enable imaging of an extensive
variety of fluorophores.
J Automated four-color fluorescence scanning allows
multiplexing of multiple targets in the same sample ensuring
accuracy of analysis, increasing throughput, and saving time.
J Storage phosphor technology delivers high-resolution
imaging and accurate quantitation of 3H, 14C, 125I, 32P, 33P,
35S, and other sources of ionizing radiation.
J Highly sensitive optics enable direct chemiluminescent
imaging without intermediate exposure to films or screens.
1 63-0055-74
Accessories
Low-fluorescence Glass Plate, Large, 3 mm thick,
33 u 42 cm
Low-fluorescence Glass Plate, Small, 3 mm thick,
20 u 36 cm
Typhoon 9410 high performance gel and blot imager that can also image
microarrays.
Quantity Code Number
Typhoon 9200
Includes: ImageQuant TL†
PAGE: 376 - CURRENCY: USD
Gel and Blot Imaging & Analysis
Product
ch10.fm Page 377 Wednesday, June 18, 2008 4:26 PM
Imaging Systems, Software, and Accessories
Typhoon Variable Mode Imager (continued)
When one of the scanning modes is selected, the appropriate
optical components are automatically activated. Typhoon
scans gels, blots, and mounted or unmounted storage phosphor
screens up to 35 u 43 cm. The instrument exhibits outstanding
linearity, quantitative accuracy, and extremely low limits of
detection.
PAGE: 377 - CURRENCY: USD
PAGE: 377 - CURRENCY: USD
PAGE: 377 - CURRENCY: USD
PAGE: 377 - CURRENCY: USD
In-lane sizing of PCR products.
GeneScan -500 ROX size standard
(yellow, Applied Biosystems),
Cy3-labeled PCR products (green),
Alfexpress Cy5 Sizer 50–500 (red,
GE Healthcare) and fluoresceinlabeled PCR products (blue) were
resolved in a 12% polyacrylamide
gel glass sandwich.
A microarray slide, spotted with cDNA
from Microarray ScoreCard control
plate and cDNA from Incyte Genomics,
Inc. was hybridized with 25 pmol of
Cy3-labeled skeletal muscle mRNA
and Cy5-labeled liver mRNA. The slide
was scanned on Typhoon 9410 at
10 µm pixel size resolution.
TECHNICAL SPECIFICATIONS
Scan area
35 u 43 cm
Sensitivity (limit of detection) Storage Phosphor: 14C (1 h exposure, 200 and
100 µm only): v 2 dpm/mm2; 32P: 5–10-fold
lower than 14C
488 nm excited fluorescence:
100 amol FAM end-labeled DNA primer in 12%
polyacrylamide gel sandwich, 0.4 mm thick
532 nm excited fluorescence:
200 amol HEX, TMR, ROX, and 400 amol FAM
end-labeled DNA primer in 12%
polyacrylamide gel sandwich, 0.4 mm thick
633 nm excited fluorescence:
200 amol Cy5 end-labeled DNA primer in 0.4
mm thick, 12% polyacrylamide gel with TBE
buffer.
Pixel size
1000, 500, 200, 100, 50, 25, and 10 µm,
selectable.
Spatial resolution
Autoradiography: 2 line pairs/mm for lines
drawn with 14C ink.
Blue-excited fluorescence: 10 line pairs/mm
Green-excited fluorescence: 10 line pairs/mm
Red-excited fluorescence: 10 line pairs/mm
Uniformity
± 5% over entire scan area.
Pixel accuracy
± 0.15%
Data format
16-bit (65 536 levels), TIFF
Linear dynamic range and Five orders of magnitude (100 000:1) with less
linearity
than 7.5% relative standard deviation for
entire dynamic range.
Red light source
Type: 10 mW Helium-Neon laser
Estimated average lifetime: z 10 000 h
Wavelength: 632.8 nm
Green light source
Type: 20 mW solid-state doubled frequency
SYAG laser
Estimated average lifetime: z 10 000 h
Wavelength: 532 nm
Blue light source
Type: 30 mW all blue lines Argon ion laser
Estimated average lifetime: z 5000 h
Wavelength: 488 nm (20 mW) and 457 nm
(4 mW)
External interface
10 Base-T Ethernet using TCP/IP protocol
Software
Scan control software for Windows operating
system.
Power requirements
115/230 V (switchable), 50–60 Hz, v 500 W
Dimensions
Instrument: 118 u 78 u 48 cm
(W u D u H)
Blue laser module: 30 u 78 u 48 cm
Safety certifications
UL 3101, CAN/CSA C22.2 No 1010.1, EN 61010-1,
EN60825-1, EN 61326, CE Marking (LVD
73/23/EEC EMC 89/336/EEC), CDRH (FDA)
Subpart 21 CFR Ch.1-Part 1040. (Please
contact your local GE Healthcare office for the
latest list of certifications.)
Gel and Blot Imaging & Analysis
Two-dimensional 2-D protein
gel stained with Deep Purple
Total Protein Stain. Protein
sample consists of HBL100
breast cell line and BT474
breast cell carcinoma. First
dimension: Immobiline
DryStrip pH 3–10 NL, 24 cm
run on Ettan IPGphor IEF
System. Second dimension:
DALT Gel 12.5 run on Ettan
DALTtwelve Large Vertical
Electrophoresis System.
Scanning: Typhoon 9400
Variable Mode Imager:
excitation, green laser
(532 nm); emission 560LP
filter, PMT 530 V, 100 µm
resolution. The gel image
shows the pH 3–8 region,
where most proteins are
present.
Choose from different Typhoon models depending on your
imaging needs. All Typhoon models come with ImageQuant TL
(see page 388 for details).
10
References
1. Tsz-Kwong, M. and Stormo, G. D. Nucleic Acids Res. 29, 2471–2478 (2001).
GE Healthcare
For more information, visit www.gelifesciences.com
377
ch10.fm Page 378 Wednesday, June 18, 2008 4:26 PM
Imaging Systems, Software, and Accessories
Typhoon Variable Mode Imager (continued)
Typhoon Trio imager delivers outstanding linearity, quantitative
accuracy, and extremely low limits of detection. Automated
multicolor scanning permits detection of multiple samples in the
same experiment, which ensures accuracy of analysis,
increasing throughput and saving time.
Typhoon Trio and Ettan DIGE Imager have been designed for
the Ettan DIGE System and allow simple scanning of largeformat gels. Typhoon can scan up to two DALT 12.5 and four
SE600 CyDye DIGE Fluor-labeled gels, and Ettan DIGE Imager
can scan one DALT 12.5 gel. Correct alignment of gels is
achieved using the appropriate Gel Alignment Guides or gel
cassettes. These greatly simplify the process of loading and
unloading gels.
SELECTION GUIDE – Typhoon Model Capabilities
Storage phosphor
Direct red-excited Direct green-excited
fluorescence
fluorescence
Typhoon model autoradiography Chemiluminescence
Direct blue-excited fluorescence
457 nm
488 nm
Microarray imaging
capability
9200
t
t
t
t
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9210
t
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t
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9400
t
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9410
t
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t
PAGE: 378 - CURRENCY: USD
Trio
Trio+
Typhoon Multislide Tray
ORDERING INFORMATION
Product
Quantity Code Number
Typhoon Multislide Tray
1 63-0054-34
For pricing information, visit www.gelifesciences.com/orderonline
Related Products
Code Number
Typhoon 9210
63-0055-76
page 376
Refer To
Typhoon 9210 with PC
Typhoon 9410
63-0055-77
63-0055-80
page 376
page 376
Typhoon 9410 with PC
63-0055-81
page 376
Typhoon Multislide tray is an accessory to the Typhoon
Instrument allowing the user to expand the microarray slide
scanning from 1 to 30 slides, as their application grows. The
tray has been designed to position slides in optimal position to
collect quantitative data.
TECHNICAL SPECIFICATIONS
Slide Dimensions
Width
Length
Thickness
24.89–25.65 mm (0.98–1.01 in)
74.50–76.45 mm (2.93–3.01 in)
0.94–1.07 mm (0.037–0.042 in)
Typhoon Multislide Tray holds up to 30 slides for microarray analysis.
Slides that do not meet the specifications above might not fit securely in the slide slots of
Typhoon Multislide Tray
378
For more information, visit www.gelifesciences.com
GE Healthcare
PAGE: 378 - CURRENCY: USD
J Typhoon Multislide Tray allows the user to scan up to 30
slides at one time on Typhoon Variable Mode Imager.
J The Typhoon Multislide Tray positions the slides at the proper
height above the glass platen of the Typhoon so that the
instrument can correctly collect data.
J Instrument control software provides simple, yet flexible
interface for the user to set up scanning parameters.
J Compatible with Code 39, Code 128, Code I2of5, and Codabar
barcodes.
J Individual files are created for each slide, with the option of
adding barcode information to the file name.
J Slides are held securely in place by compression pads located
on the lid while being scanned.
PAGE: 378 - CURRENCY: USD
10
J Provides optimized detection of Cy2, Cy3, and Cy5 CyDye
DIGE Fluor minimal and saturation dyes, enabling
visualization of up to three differently labeled samples on a
single gel.
J Exceptional signal-to-noise increases the number of
statistically significant differences detected.
J Consistent point-light illumination produces high-quality
uniform images and reduces photo-bleaching.
J Gels are scanned between glass plates, preventing drying
and shrinkage and allowing further running and rescanning if
required.
Typhoon Trio imager unites the ability to detect an extensive
variety of fluors with proven storage phosphor
autoradiography technology and direct imaging of
chemiluminescence. Powerful excitation sources and
innovative high quality confocal optics allow sensitive detection
of low-abundance targets.
PAGE: 378 - CURRENCY: USD
Gel and Blot Imaging & Analysis
Typhoon Trio imager is enabled for 2-D DIGE provides multicolor
scanning and optimized detection of CyDye DIGE Fluor
dye-labeled proteins.
ch10.fm Page 379 Wednesday, June 18, 2008 4:26 PM
Imaging Systems, Software, and Accessories
Storm
ORDERING INFORMATION
Product
Quantity Code
Number
Storm 860 & ImageQuant TL *
1 63-0035-63
Includes: ImageQuant TL, scanner control software, Image Eraser, SCSI cable and
terminator. Requires approved computer.
Storm 840 & ImageQuant TL *
1 63-0035-58
PAGE: 379 - CURRENCY: USD
PAGE: 379 - CURRENCY: USD
PAGE: 379 - CURRENCY: USD
PAGE: 379 - CURRENCY: USD
Storm 860 Gel and Blot Imaging System offers filmless autoradiography,
fluorescence and chemifluorescence imaging.
J Large format imaging systems for filmless
autoradiography, fluorescence, and chemifluorescence
imaging.
J Filmless autoradiography based on proven PhosphorImager
technology.
J Direct red- and blue-excited fluorescence enable rapid and
accurate quantitation using fluorescence- and
chemifluorescence-based methods.
J Five orders of linear dynamic range ensure accurate,
publication-quality results on the first exposure.
Storm Gel and Blot Imaging System delivers proven
PhosphorImager capability for autoradiography, direct
fluorescence for nucleic acid and protein gel analysis, and
chemifluorescence for fast blot analysis. Storm uses storage
phosphor screens instead of film to deliver high-resolution
imaging and accurate quantitation of 14C, 3H, 125I, 32P, 33P, 35S,
and other sources of ionizing radiation.
Includes: ImageQuant TL, scanner control software, Image Eraser, SCSI cable and
terminator. Requires approved computer.
Workstation scanner with monitor
1 28-4086-78
For pricing information, visit www.gelifesciences.com/orderonline
* Does not include phosphor screens and exposure cassettes.
For ordering of phosphor screens, please refer to Selection Guide on see page 381.
Related Products
Refer To
Storage Phosphor Screens and Cassettes
Image Eraser
page 381
page 382
ImageQuant TL
Blotting Membranes
page 388
page 324
Nucleic Acid Labeling and Detection
Protein Labeling and Detection
page 330
page 353
SDS-PAGE Electrophoresis Units
Nucleic Acid Electrophoresis Units
page 283
page 300
Amersham Radiochemicals & Radiation Safety
Chapter 3
Amersham ECL Plex Western Blotting Combination Packs
Amersham ECL Plex CyDye-conjugated Antibodies
page 354
page 354
The system’s wide exposure range and accurate signal
quantification yield publication-quality images on the first
exposure. Storage phosphor screens are reusable and are not
degraded by repeated exposure to laboratory levels of
radioactivity. To reuse, simply expose a screen to the extrabright light of Image Eraser light box (included with Storm
systems). With direct fluorescence, Storm enables visualization
and analysis of nucleic acid and protein gels just minutes after
electrophoresis is complete. Gels are soaked in dye solution and
then placed in the scanner for analysis. Molecules labeled with
Cy5 can also be directly detected.
Gel and Blot Imaging & Analysis
Includes: ImageQuant TL, scanner control software, Image Eraser, SCSI cable and
terminator. Requires approved computer.
Storm 820 & ImageQuant TL *
1 63-0035-53
10
For chemifluorescence-based methods, Storm reads blots in
minutes, without exposure to film. Quantitation is simplified
because, unlike film, Storm exhibits a linear response to
fluorescent signal intensities.
Storm scans gels, blots, or mounted and unmounted storage
phosphor screens up to 35 u 43 cm. All Storm systems come
with ImageQuant TL.
GE Healthcare
For more information, visit www.gelifesciences.com
379
ch10.fm Page 380 Wednesday, June 18, 2008 4:26 PM
Imaging Systems, Software, and Accessories
Storm (continued)
SELECTION GUIDE – Storm System Capabilities
t
840
t
860
t
t
t
t
TECHNICAL SPECIFICATIONS
Scan area
Sensitivity (limit of
detection)
Pixel size
Spatial resolution
Uniformity
Pixel accuracy
Data format
Linear dynamic range
and linearity
Light source
External interface
Software
Dimensions
(W u D u H)
Safety certifications
Atlas Human cDNA array probed with 33P-labeled cDNA. The hybridized array,
containing 588 unique targets, was exposed to a storage phosphor screen for
2 days and imaged using Storm 840.
PAGE: 380 - CURRENCY: USD
Power requirements
Gene Discovery Array Human I probed with 32P-labeled human cDNA. The
22 u 22 cm membrane array contained 18 394 non-redundant cDNA
clones. The array was exposed to a storage phosphor screen for one hour
and imaged using Storm 820.
380
For more information, visit www.gelifesciences.com
GE Healthcare
PAGE: 380 - CURRENCY: USD
35 u 43 cm
v 2 dpm/mm2 for 14C (200 and 100 µm only) using
GP screen in a one-hour exposure.
200, 100, and 50 µm, selectable. The scanning
laser beam is approximately 50 µm in diameter.
Autoradiography: 2 line pairs/mm for lines drawn
with 14C ink.
Blue-excited fluorescence, chemifluorescence:
2 line pairs/mm
Red-excited fluorescence: 4 line pairs/mm
± 5% relative standard deviation
± 0.15%
16-bit (65 536 levels), TIFF
5 orders of magnitude (100 000:1) with less than
7.5% relative standard deviation for entire
dynamic range.
Storm 820, 840, and 860 use a red laser diode (635
nm). Storm 840 and 860 also use a blue LED (450
nm).
SCSI
Scan control software for Windows operating
system.
Storm: 115/230 V, 50–60 Hz, v 150 W
ImageEraser: 115/230 V, 50–60 Hz, v 150 W
Storm: 76 u 76 u 38 cm
ImageEraser: 42 u 32 u 63.5 cm
UL 3101, CAN/CSA C22.2 No 1010.1, EN 61010-1,
EN60825-1, EN 61326, CE Marking (LVD
73/23/EEC EMC 89/336/EEC), CDRH (FDA)
Subpart 21 CFR Ch.1-Part 1040. (Please contact
your local GE Healthcare office for the latest list
of certifications.)
PAGE: 380 - CURRENCY: USD
10
820
PAGE: 380 - CURRENCY: USD
Gel and Blot Imaging & Analysis
Storage phosphor Direct red-excited Direct blue-excited
Storm Model autoradiography
fluorescence
fluorescence
ch10.fm Page 381 Wednesday, June 18, 2008 4:26 PM
Imaging Systems, Software, and Accessories
Storage Phosphor Screens and Cassettes
ORDERING INFORMATION
Product
Quantity*
Code Number
Mounted, GP 20 u 25 cm
Mounted, GP, 20 u 25 cm, Screen & Cassette
Mounted, GP, 20 u 25 cm, Screen Only
1 63-0034-89
1 63-0034-88
Mounted, GP 35 u 43 cm
Mounted, GP, 35 u 43 cm, Screen & Cassette
Mounted, GP, 35 u 43 cm, Screen Only
Unmounted, GP, 20 u 25 cm, Screen Only
Unmounted, GP 35 u 43 cm
Unmounted, GP, 35 u 43 cm, Screen & Cassette
PAGE: 381 - CURRENCY: USD
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PAGE: 381 - CURRENCY: USD
Unmounted, GP, 35 u 43 cm. Screen Only
Tritium Screen (19 u 24 cm)
Storage phosphor screens require less exposure time and are 10 to 100 times
more sensitive than film.
J Requires approximately one-tenth the exposure time of
traditional autoradiography film.
J 10 to 100 times more sensitive than film, depending on
isotope and sample type.
J Wide linear dynamic range (5 orders of magnitude) enables
quantitation and visualization of both weak and strong
signals in a single exposure.
J Reusable screens do not require chemicals, darkroom, or
other special treatment for use.
J Each step can be performed at the lab bench under normal
lighting conditions and at room temperature.
J Results are digitized using a storage phosphor imaging
system (e.g., Typhoon, Storm, or PhosphorImager) and
quantitated using ImageQuant software.
Storage phosphor screens capture latent images produced by
ionizing radiation (X-rays, b, and g emissions from isotopes such
as 14C, 3H, 125I, 131I, 32P, 33P, 35S, etc.). Upon laser-induced
stimulation, light is emitted from the storage phosphor screen in
proportion to the amount of radioactivity in the sample. The
resulting digital image allows for quantitation of subtle signal
intensity differences over a wide dynamic range using storage
phosphor imaging systems such as Typhoon, Storm, and
PhosphorImager. Typically, 50% to 90% less exposure time is
required compared with an equivalent exposure to
conventional film. Publication quality images are often obtained
in a single exposure.
1 63-0034-86
1 63-0034-87
1 63-0034-79
1 63-0034-80
Mounted, Tritium, 19 u 24 cm, Screen Only
Unmounted, Tritium, 19 u 24 cm, Screen Only
1 63-0035-50
1 63-0035-49
Kit, Tritium, 19 u 24 cm, 1 mounted & 4 unmounted
Backing Plate, Tritium, 19 u 24 cm
1 63-0035-51
1 63-0035-48
Exposure Cassettes for mounted screens
Exposure Cassette, Mounted Screen, 20 u 25 cm
1 63-0035-46
Exposure Cassette, Mounted Screen, 35 u 43 cm
1 63-0035-47
Exposure Cassettes for unmounted screens
Exposure Cassette, Unmounted Screen, 20 u 25 cm
1 63-0035-44
Exposure Cassette, Unmounted Screen, 35 u 43 cm
1 63-0035-45
For pricing information, visit www.gelifesciences.com/orderonline
*Contact your local sales representative for multiple screen pricing
Related Products
Refer To
Typhoon Multislide Tray
Storm
page 378
page 379
ImageQuant TL
Amersham Blotting, Labeling & Detection
page 388
Chapter 9
Amersham Radiochemicals & Radiation Safety
Chapter 3
Screen Types
General Purpose (GP) Screens: GP screens are reliable for a
wide variety of applications and can be used with 32P, 125I, 35S,
33P, and 14C. The durable cellulose acetate coating and the
phosphor layer formulation makes the GP screen ideal for 32P
and 125I detection and quantitation. This is the screen of choice
for 32P Northerns and Southerns and 125I Western blots and
gels.
Gel and Blot Imaging & Analysis
Unmounted, GP 20 u 25 cm
Unmounted, GP, 20 u 25 cm, Screen & Cassette
1 63-0034-82
1 63-0034-81
10
Tritium (TR) Screens: To detect the weak energy of the 3H
signal, Tritium screens are constructed without a protective
cellulose acetate overlay. The highly sensitive screens eliminate
the need for fluorographic reagents. Best results are obtained
when the 3H signal is on the surface of the sample and available
to penetrate the screen.
Storage phosphor screens are reusable and are not degraded
by repeated exposure to laboratory levels of radioactivity. To
reuse, simply expose a screen to the extra-bright light of
ImageEraser light box (included with some phosphor storage
imaging systems from GE Healthcare).
Screen Styles
Mounted screens are permanently fixed to an aluminum
backing plate. They require an exposure cassette. Older
PhosphorImager Systems require mounted screens. Mounted
screens are also compatible with Typhoon and Storm systems.
Unmounted screens have a flexible backing and are compatible
only with Typhoon and Storm systems. They are available with
standard autoradiography cassettes.
GE Healthcare
For more information, visit www.gelifesciences.com
381
ch10.fm Page 382 Wednesday, June 18, 2008 4:26 PM
Imaging Systems, Software, and Accessories
Storage Phosphor Screens and Cassettes (continued)
SELECTION GUIDE BY INSTRUMENT COMPATIBILITY – Storage Phosphor Screens
Small Phosphor Screen
20 u 25 cm
Large Phosphor Screen
35 u 43 cm
Screen &
Cassette
Screen only
Screen &
Cassette
Screen only
Screen &
Cassette
Macroarray
Phosphor
Screen
Half-size Screen
17.5 u 43 cm
Screen only Screen only
Tritium Screen
19 u 24 cm
Screen &
Cassette
Screen kit
PhosphorImager
(small format)
PSI, PSF
Mounted
GP*
63-0034-89
63-0034-88
-
-
-
-
-
63-0035-50
63-0035-51
(1 mounted, 4
unmounted)
PhosphorImager
(large format)
400, 425, 445
Mounted
GP*
63-0034-89
63-0034-88
63-0034-82
63-0034-81
63-0034-85
-
-
63-0035-50
63-0035-51
(1 mounted, 4
unmounted)
Storm
820, 830, 840, 860
Mounted
GP*
63-0034-89
63-0034-88
63-0034-82
63-0034-81
63-0034-85
-
-
63-0035-50
Unmounted
GP*
63-0034-86
63-0034-87
63-0034-79
63-0034-80
63-0034-84
63-0034-83
63-0034-90
Mounted
GP*
63-0034-89
63-0034-88
63-0034-82
63-0034-81
63-0034-85
-
-
Unmounted
GP*
63-0034-86
63-0034-87
63-0034-79
63-0034-80
63-0034-84
63-0034-83
63-0034-90
Typhoon
8600, 8610, 9200,
9210, 9400, 9410,
Trio, Trio+
63-0035-51
(1 mounted, 4
unmounted)
63-0035-49
63-0035-50
63-0035-51
(1 mounted, 4
unmounted)
63-0035-49
-
*GP (General Purpose screen): For use with 125I, 32P, 33P, 35S, and 14C
Image Eraser
Image Eraser is a specially designed light box used to erase
latent images formed on storage phosphor screens. It reduces
the background level prior to sample exposure.
ORDERING INFORMATION
Product
Quantity Code Number
Image Eraser
1 63-0007-83
For pricing information, visit www.gelifesciences.com/orderonline
Related Products
Refer To
Storage Phosphor Screens and Cassettes
page 381
TECHNICAL SPECIFICATIONS
Power requirements
Dimension (W u H u D)
115/230 V (switchable); 50/60 Hz, v 150 W
42 u 63.5 u 32 cm
PAGE: 382 - CURRENCY: USD
J Allows for quick and complete erasure of storage screens.
J Compatible with all PhosphorImager, Storm, and Typhoon
imagers.
J Accepts any storage phosphor screen based on BaBrF(Eu2+)
chemistry up to a maximum size of 35 u 43 cm.
PAGE: 382 - CURRENCY: USD
10
Type
PAGE: 382 - CURRENCY: USD
Gel and Blot Imaging & Analysis
Instrument
PAGE: 382 - CURRENCY: USD
382
For more information, visit www.gelifesciences.com
GE Healthcare
ch10.fm Page 383 Wednesday, June 18, 2008 4:26 PM
Imaging Systems, Software, and Accessories
DIGE approved
Ettan DIGE Imager
ORDERING INFORMATION
PAGE: 383 - CURRENCY: USD
PAGE: 383 - CURRENCY: USD
PAGE: 383 - CURRENCY: USD
J High resolution imaging from a unique progressive
scanning CCD; for differential protein expression using
Ettan DIGE and relative quantitation Western Blotting with
ECL Plex.
J Optimized and balanced fluorescence detection in
multiplexed experiments, ensures minimal experimental bias,
superior sensitivity and signal-to-noise.
J High sensitivity for imaging faint spots combined with a linear
dynamic range greater than 3.5 orders of magnitude to allow
accurate quantitation of low and high concentrations
simultaneously.
J Dedicated, removable sample handling cassettes for naked
gels and membranes, as well as DALT and SE 600 series gel
formats.
J Very low noise from the innovative use of advanced low
fluorescent materials within the optics path and sample
cassette.
J Shielded optics to minimize sample heating and fluorophor
degradation during scanning.
J Sealed environment for sample protection during the
scanning of both wet and dry samples.
J Cost-effective fluorescent imager with a wide application
range and a powerful but low-cost light (excitation) source
that can be easily replaced by the user.
The Ettan DIGE Imager is a scanning CCD camera designed for
applications in the life sciences. In particular, it has been
engineered to create high-quality images of 2-D Fluorescence
Difference Gel Electrophoresis (DIGE) gels. By combining very
high resolution with precise motion control, the Ettan DIGE
Imager produces accurate multichannel images of your Cy2-,
Cy3-, and Cy5-labeled gel. It has also been designed to image a
wide range of other fluorescent gel applications, including the
ECL Plex Western Blotting Detection System, see page 353.
Quantity Code Number
Ettan DIGE Imager, including installation kit*
PC including keyboard and mouse, for Windows XP†
1 63-0056-42
1 11-0036-58
Monitor, 20 inch†
Ettan DIGE Imager Cassette, 276 u 212 mm, for DALT
gel sandwiches
1 11-0034-98
1 11-0027-04
Ettan DIGE Imager Cassette, with low-fluorescent
glass, for naked gels
1 11-0027-33
Ettan DIGE Imager Cassette, 180 u 160 mm, for SE 600
series gel sandwiches
Installation kit (cable, CD, instructions)
1 11-0027-32
1 11-0034-81
For pricing information, visit www.gelifesciences.com/orderonline
* Order at least one cassette with the Ettan DIGE Imager
† We highly recommend using our tested and approved computer and monitor
Related Products
Refer To
DeCyder 2-D Differential Analysis Software v6.5
page 265
DeCyder Extended Data Analysis (EDA) Software v1.0
ImageMaster 2D Platinum v6.0
page 266
page 268
ImageQuant TL
page 388
Ettan DIGE Imager Cassette,
180 u 160 mm, for SE 600 series gel
sandwiches.
Ettan DIGE Imager Cassette,
276 u 212 mm, for DALT gel
sandwiches.
Gel and Blot Imaging & Analysis
PAGE: 383 - CURRENCY: USD
Product
10
Ettan DIGE Imager Cassette, with lowfluorescent glass, for naked gels.
Multichannel image of a 2-D DIGE gel acquired with the Ettan DIGE Imager.
GE Healthcare
For more information, visit www.gelifesciences.com
383
ch10.fm Page 384 Wednesday, June 18, 2008 4:26 PM
Imaging Systems, Software, and Accessories
J CCD-based imaging systems cover the full range of gel
documentation, fluorescence, and chemiluminescence
applications.
J You can choose from entry-level, low-cost systems to highperformance expert instruments.
J Instruments can be easily updated from the simplest to the
most sophisticated as needs evolve.
J Camera cabinet, common to most of the range, includes
white light and UV excitation, with epi-illumination options.
J Imagers are compatible with image analysis software:
ImageQuant TL and ImageMaster 2D Platinum v6.0.
GE Healthcare has combined its expertise in imaging and life
science research applications with the latest developments in
digital imaging to create tools designed specifically for the
laboratory researcher. These instruments, combined with our
reagents, software, and support, provide applications-oriented
system solutions that enable scientists in all aspects of gene
and protein discovery. These proprietary CCD and camera
combinations have been selected to ensure that you obtain the
optimal results from your experiments.
SELECTION GUIDE BY APPLICATION – ImageQuant Imagers for Chemiluminescence, Fluorescence, and Densitometric Applications
Chemiluminescence
Fluorescence
Densitometry
Fluorescence
Densitometry
PAGE: 384 - CURRENCY: USD
Highly sensitive,
high-resolution
quantitation
Highest sensitivity,
high-resolution
quantitation
ImageQuant 150*
8 bit, 10 megapixels
ImageQuant 300
10 bit, 0.8 megapixels
ImageQuant 350
16 bit, 2 megapixels
Imagequant RT ECL
16 bit, 4 megapixels
* See www.gelifesciences.com for details
For more information, visit www.gelifesciences.com
GE Healthcare
PAGE: 384 - CURRENCY: USD
Documentation
with
quantitation
PAGE: 384 - CURRENCY: USD
Documentation
without
quantitation
10
384
PAGE: 384 - CURRENCY: USD
Gel and Blot Imaging & Analysis
ImageQuant Imagers
ch10.fm Page 385 Wednesday, June 18, 2008 4:26 PM
Imaging Systems, Software, and Accessories
ImageQuant RT ECL
ORDERING INFORMATION
Product
Quantity Code Number
ImageQuant RT ECL
ImageQuant RT ECL with ImageQuant TL software
ImageQuant RT ECL with PC and ImageQuant TL software
1 28-9043-15
1 28-9048-79
Contact local
sales office
For pricing information, visit www.gelifesciences.com/orderonline
ImageQuant TL
Refer To
page 388
Introduction to Amersham ECL Western Blotting Systems
page 353
ImageMaster 2D Platinum v6.0
page 268
The standard features of the cabinet, combined with affordable
options, mean any sample can be imaged—white and UV, trans
and epi illumination. Special sources are available for TLC, GFP,
and blue light conversion.
J Real-time refresh rate provides ease of use and rapid image
capture.
J Combinations of lens, camera, and appropriate Amersham
ECL reagents deliver outstanding chemiluminescent imaging
sensitivity with fast exposure times.
J Wide dynamic range, 16-bit cooled CCD camera ensures high
sensitivity and avoids saturation of images.
J Darkroom cabinet can be configured for all commonly used
lighting options.
J Cabinet design is flexible enough to allow for modifications if
your requirements change or to take advantage of new
opportunities.
J Designed to complement GE Healthcare's comprehensive
range of Western blotting solutions.
Add ImageQuant TL analysis software, and images can quickly
be converted from data to information.
10
PAGE: 385 - CURRENCY: USD
PAGE: 385 - CURRENCY: USD
PAGE: 385 - CURRENCY: USD
PAGE: 385 - CURRENCY: USD
ImageQuant RT ECL system is designed as a general-purpose,
fully featured imager, but with particular focus on highsensitivity chemiluminescence imaging. If Western blotting is
the main use for your instrument, then the ImageQuant RT ECL
is the best imager in the range for you.
Gel and Blot Imaging & Analysis
Related Products
GE Healthcare
For more information, visit www.gelifesciences.com
385
ch10.fm Page 386 Wednesday, June 18, 2008 4:26 PM
Imaging Systems, Software, and Accessories
ImageQuant 350
ORDERING INFORMATION
Product
ImageQuant 350 Standard Z
Quantity Code Number
1 28-9261-75
Accessories
Motorized lenses
f/2.8 zoom lens 28-70 mm Z
1 28-9265-84
(suitable for ImageQuant models 350, 400, and RT ECL)
1 28-9265-85
ImageQuant 350 allows high-resolution scans of monochromatic fluorescence
gels, Coomassie Blue-stained gels, and other colorimetric detection methods.
f/1.4 fixed lens 50 mm Z
(suitable for ImageQuant models 350, 400, and RT ECL)
1 28-9265-86
J Cost-efficient, sensitive, and quantitative imaging of
Western blots and other monochromatic fluorescence and
colorimetric imaging applications.
J 16-bit dynamic range minimizes the need for repeated
acquisitions at multiple exposures because faint and dark
bands can be accurately captured and analyzed in the same
image.
J High resolution, 1.92 megapixel camera delivers accurate
quantitation of closely spaced bands in complex protein
samples for gels and films up to 21 x 16 cm.
J High sensitivity enables detection and quantitation of lowabundant proteins on a Western blot.
J Simplified workflow eliminates the time, effort, and
environmental waste of the darkroom.
J Easy-to-use system allows rapid switching between
applications such as analyzing blots or imaging gels,
microplates, or culture dishes.
Manual lenses
The ImageQuant 350 is a compact, easily installed system,
designed for easy exchange of cameras, lenses, and filters. The
standard system includes one manual fixed lens and one
manual zoom lens, and the motorized system includes one
motorized fixed lens and one manual zoom lens.
1 63-0057-14
1 28-9277-27
f/0.95 fixed lens 50 mm Z
f/0.95 fixed lens 25 mm Z
1 28-9266-05
1 28-9266-06
Filters
Emission Filter 590/55 nm Z
1 28-9272-77
for ethidium bromide, Coomassie Blue, SYPRO Orange
Emission Filter 537/35 nm Z
for GFP, SYBR Safe, SYBR Gold, Fluorescein, SYBR Green
1 28-9272-78
Emission Filter 460/40 nm Z
for Hoechst 33258, blue dye, or carbohydrate gels
1 63-0056-65
Emission Filter 620/40 nm Z
for SYPRO Red, SYPRO Ruby, Texas Red, Rhodamine
1 63-0056-64
Other Accessories
Dual Epi UV Lights, 254 nm, 110 VAC
Dual Epi UV Lights, 254 nm, 220 VAC
1 63-0056-56
1 63-0057-10
Dual Epi UV Lights, 365 nm, 110 VAC
Dual Epi UV Lights, 365 nm, 220 VAC
1 63-0056-57
1 63-0057-11
Dual Epi Blue Light Clip-ons, 460 nm
Thermal Printer
1 63-0056-58
1 63-0056-69
High-gloss thermal paper
5 63-0056-70
for best image quality
High-contrast thermal paper
4 63-0056-71
for good image quality
For pricing information, visit www.gelifesciences.com/orderonline
Related Products
ImageQuant TL
Amersham ECL Western Blotting System
Refer To
page 388
page 357
The imager comes with an ethidum bromide (EtBr) filter as
standard; other filters are available as options.
Using ImageQuant TL analysis software (see page 388) with
ImageQuant 350 provides high levels of automation, accuracy
and reduces analysis time to a minimum.
The standard UV transilluminator and the white light source (a
fold-down table), as well as any optional light sources, are
housed dust-free inside the cabinet. The UV transilluminator
can be operated at either 302 nm or 365 nm at low or high
intensity. For cutting sample bands from the gel, the
transilluminator slides out of the cabinet.
386
For more information, visit www.gelifesciences.com
GE Healthcare
PAGE: 386 - CURRENCY: USD
ImageQuant 350 is a high-performance 16-bit cooled CCD
camera-based system designed for a wide range of
monochromatic imaging applications. ImageQuant 350 is wellsuited for quantitative analysis of Western blots. The system is
also fully capable of imaging monochromatic fluorescent gels
and membranes, Coomassie Blue-stained gels, and other
colorimetric detection methods. Cultures and microplate-based
assays can also be imaged. Whereas film is valuable and
convenient for certain applications, ImageQuant 350 supports a
more quantitative approach for a broader range of applications
without compromising sensitivity, resolution, or simplicity of
operation. Most commands are performed by pushing a single
button, thereby supporting many different applications in a
day.
f/1.4 fixed lens 50 mm
f/1.8 fixed lens 28 mm
PAGE: 386 - CURRENCY: USD
f/1.8 fixed lens 28 mm Z
(suitable for ImageQuant models 350, 400, and RT ECL)
PAGE: 386 - CURRENCY: USD
10
ImageQuant 350 with Motorized Fixed Lens Z
1 28-9272-95
Includes: Two lenses: motorized fixed f/1.8 28 mm, manual zoom f/2.8 28-70 mm,
302/365 nm dual wavelength transilluminator, Image Capture Software, without
computer and monitor
PAGE: 386 - CURRENCY: USD
Gel and Blot Imaging & Analysis
Includes: Two lenses: manual fixed f/1.8 28 mm, manual zoom f/2.8 28-70 mm, 302/365 nm
dual wavelength transilluminator, Image Capture Software, without computer and
monitor
ch10.fm Page 387 Wednesday, June 18, 2008 4:26 PM
Imaging Systems, Software, and Accessories
ImageQuant 300
ORDERING INFORMATION
Product
Quantity Code Number
ImageQuant 300
ImageQuant 300 with ImageQuant TL software
1 63-0056-50
1 63-0056-51
ImageQuant 300 with PC and ImageQuant TL software
1 63-0056-52†
For pricing information, visit www.gelifesciences.com/orderonline
region - please contact your local GE Healthcare office for
details.
Related Products
Refer To
PAGE: 387 - CURRENCY: USD
PAGE: 387 - CURRENCY: USD
ImageQuant TL
ImageMaster 2D Platinum v6.0
J An affordable, high-performance, multipurpose gel
documentation and image analysis system.
J Full-featured darkroom cabinet for a complete range of
lighting possibilities.
J Wide dynamic range camera to avoid saturation problems.
J High-resolution imaging over entire area of full-size
transilluminator.
J Upgradeable to cooled CCD system for high-sensitivity
chemiluminescence.
page 388
page 268
ImageQuant 300 is well suited for the busy laboratory imaging
colored and fluorescent samples requiring documentation and
analysis. The standard features of the cabinet, combined with
affordable options, mean any sample can be imaged—white
and UV, trans and epi illumination. Special sources are available
for TLC, GFP, and blue light conversion.
Add ImageQuant TL analysis software, and images can quickly
be converted from data to information. These powerful
combinations will support most of your imaging needs. If in the
future your requirements expand, then add a new camera to
access high-sensitivity chemiluminescence or new CCD
technology—without the need to buy a new instrument.
ImageScanner III
ORDERING INFORMATION
PAGE: 387 - CURRENCY: USD
PAGE: 387 - CURRENCY: USD
Product
Quantity Code Number
ImageScanner III
1 28-9076-07
Including drivers for Windows 2000 and XP, and Adobe Photoshop; including LabScan
6.0 and calibration step tablets #2 and #3, and a 3 m USB cable.
Gel and Blot Imaging & Analysis
† Availability varies by
10
For pricing information, visit www.gelifesciences.com/orderonline
Related Products
ImageMaster 2D Platinum v6.0
ImageScanner III, a versatile scanner for all densitometric applications in the life
sciences, exhibits linearity above 3.4 OD.
Code Number
Refer To
page 268
ImageScanner III combines very high resolution with an
exceptional optical density range, enabling accurate and
sensitive densitometric quantitation of gels, blots, membranes
and slides. Samples can be scanned in either reflection or
transmission mode. ImageScanner is compatible with
ImageMaster software. The scanner can be used also for highresolution scanning of documents, photos, and films for desktop
publishing.
J Highly sensitive flatbed scanner for for most common
nonfluorescent densitometric applications in the life
sciences, especially electrophoresis evaluation.
J Linearity above 3.4 OD.
J 16-bit pixel depth provides accurate quantitation.
J Provides high sensitivity of very faint images without
additional filters.
ImageMaster 2D Platinum v6.0
For main product entry, see page 268.
GE Healthcare
For more information, visit www.gelifesciences.com
387
ch10.fm Page 388 Wednesday, June 18, 2008 4:26 PM
Imaging Systems, Software, and Accessories
ImageQuant TL
ImageQuant TL is the most automatic and easy to use general
image analysis software. It offers four separate functional areas
within one product:
J 1-D electrophoresis gels.
J Dot blots, microplates, and basic arrays.
J Colony counting and measurement of 2-D spots and other
image features.
J Toolbox to analyze gels with user-defined objects.
ORDERING INFORMATION
Product
Quantity Code Number
ImageQuant TL, DVD, and quick start guide
ImageQuant TL, single user license
1 28-9194-45
1 28-9236-62
ImageQuant TL, 5-user network license
ImageQuant TL, 10-user network license
1 28-9206-39
1 28-9236-57
ImageQuant TL User Manual
1 28-9175-41
For pricing information, visit www.gelifesciences.com/orderonline
Contact your local sales office for special upgrade offerings and other packages
Related Products
Code Number
Typhoon Variable Mode Imager
Storm
ImageScanner III
Refer To
page 376
page 379
page 387
28-9076-07
Its high level of automation throughout allows for fully
automatic analysis of 1-D gels—including lane creation,
background subtraction, band detection, molecular weight
calibration, quantity calibration, and normalization—in a matter
of a few seconds. This ensures high reproducibility.
Colony Counting and 2-D spot measurement
We have incorporated the 2-D spot detection algorithm of
ImageMaster into ImageQuant TL to enable accurate and
reproducible identification, measurement and background
subtraction on images containing colonies and spots. This can
be very useful for colony counting, detection, and
measurement of 2-D spots and measurement of other image
features. Currently, the colony counting module does not
support multichannel files.
Automated analysis of 1-D electrophoresis gel images.
IQTools and FluorSep
These two modules allow further image manipulations
including:
J Image data export to Excel for 3-D charting.
J Channel flickering.
J Renaming of individual channels.
J Fluorescent cross-talk removal.
ImageQuant TL accepts files from all GE Healthcare imaging
equipment, including Typhoon, Storm, ImageScanner III, and
VDS-CL, and accepts TIFF files from third party instruments. The
software fully supports multicolor files with up to 4 channels (.ds
files). Operates under Microsoft Windows 2000, and XP.
Contains 1 CD with software and electronic documentation, and
a printed quick start manual.
For a free 14 days trial download, see
www.gelifesciences.com/iqtl.
Analysis Toolbox
The Toolbox modules can be used to analyze samples that do
not fit any of the above categories. ImageQuant Solutions users
will find all the familiar tools they used with previous versions of
ImageQuant in this module. Toolbox requires the manual
definition of objects for quantitation.
J Palette of tools including rectangle, ellipse, polygon, freeform
and autotracer.
J Auto-tracer objects can be further edited.
J Wide choice of background correction methods.
J Wide choice of measurement statistics.
388
For more information, visit www.gelifesciences.com
GE Healthcare
PAGE: 388 - CURRENCY: USD
Array analysis
ImageQuant TL can be used for dot and slot blots, microplates,
and other arrays and grids.
J Automatic creation of grids with up to 1536 independent cells
allows fast analysis of high throughput microplates.
J Grid can be stretched or distorted.
J Spot size and position adjustable for individual spots. Both
features help to analyze distorted arrays, e.g. membrane
arrays or manually spotted arrays.
J Normalization, presence flagging, and quality factor allow for
easy assessment of results.
PAGE: 388 - CURRENCY: USD
1-D Analysis
ImageQuant TL has a redesigned interface, which increases
automation and ease of use.
J Fully automatic lane and band detection and quantitation for
up to four channels.
J Compatible with multitiered gels.
J Advanced automatic background subtraction including
''rolling disk'' method.
J De-smiling of gels and de-grimacing of lanes.
J Normalization and quantity calibration with many regression
curves.
J Molecular weight calibration across up to four channels.
PAGE: 388 - CURRENCY: USD
10
Automation for fast and consistent results.
Advanced algorithms for delivery of accurate results.
Editing tools to crop, rotate, and filter images.
Support of multicolor files.
Manual override capability.
PAGE: 388 - CURRENCY: USD
Gel and Blot Imaging & Analysis
J
J
J
J
J
ch10.fm Page 389 Wednesday, June 18, 2008 4:26 PM
Autoradiography Films, Screens, and Accessores
Autoradiography
For more than two decades, Amersham products from GE
Healthcare have been used in autoradiography applications
within biomedical research. Our expertise in the labeling and
detection of biomolecules has enabled us to continue to supply
and support a range of innovative products, including
Amersham Hyperfilm.
SELECTION GUIDE – Autoradiography Methods and Materials for Life Science Applications
Application
Result Required
Label
Method
PAGE: 389 - CURRENCY: USD
Support products
Hyperfilm MP
Sensitize, Hyperscreen
Nonradioactive; quantitation
ECL, ECF,
CDP-Star
Preflash and direct detection
Hyperfilm ECL
Sensitize
High speed and sensitivity;
accurate quantitation
32 P
Preflash with screens at -70°C
Hyperfilm MP
Sensitize, Hyperscreen
Nonradioactive, quantitation
ECL, ECF,
CDP-Star
Preflash and direct detection
Hyperfilm ECL
Sensitize
Dideoxy sequencing
High speed
32 P
Direct autoradiography
Hyperfilm MP
Cycle sequencing
Good resolution and speed for
routine work
33 P
Direct autoradiography
Hyperfilm MP
Protein synthesis,
lysates
Maximum speed and
good resolution
35 S/14 C/
3H/ 125 I
Preflash and fluorography
at -70°C
Hyperfilm MP
Direct autoradiography
Hyperfilm 3H
Western blots
Maximum speed
and sensitivity
125I/ 35S/14C
Preflash and screens at -70°C
Hyperfilm MP
Sensitize, Hyperscreen
Nonradioactive/
maximum sensitivity
ECL,
Preflash and direct detection
ECL Advance,
ECL Plus
Hyperfilm ECL
Sensitize
In situ hybridization
Macroscale in situ optimization of
parameters
32 P
Direct autoradiography
Hyperfilm MP
Receptor studies
Localization at cellular level
– high resolution
33 P/ 35S
Micro autoradiography
Hypercoat emulsion LM-1
Localization at sub-cellular level
– high resolution
125I/ 3H
Micro autoradiography
Hypercoat emulsion EM-1
Localization at sub-cellular level
– intermediate resolution
125I/ 3H
Direct autoradiography
Hyperfilm 3H
Optimum resolution
125I/ 3H
Direct autoradiography
Hyperfilm 3H
Slot blots
Whole body
autoradiography
Amplify, Sensitize
10
PAGE: 389 - CURRENCY: USD
PAGE: 389 - CURRENCY: USD
PAGE: 389 - CURRENCY: USD
Film
Gel and Blot Imaging & Analysis
Southern/Northern blots; High speed, resolution, and sensitivity; 32P, 33P, 35S Preflash with screens at -70°C
Colony/plaque blots
Accurate quantitation
GE Healthcare
For more information, visit www.gelifesciences.com
389
ch10.fm Page 390 Wednesday, June 18, 2008 4:26 PM
Autoradiography Films, Screens, and Accessories
The high-performance emulsion in Hyperfilm ECL is sensitive to
both blue and green light chemiluminescence systems. The
emulsion of this double-coated film provides improved clarity
and sensitivity for the detection of chemiluminescent-labeled
samples, making it suitable for ECL, ECL Plus and CDP-Star and
all other chemiluminescence systems.
Western blot of Hdmx
immunoprecipitated from MCF7 cell
extracts. Co-immunoprecipitated p53
was detected with Amersham
Hyperfilm ECL. Exposure 30 s, primary
antibody 1/1000 mouse anti-p53
monoclonal, secondary antibody
1/10 000 donkey anti-mouse.
Results supplied by Pamela Robertson,
Surgery and Molecular Oncology,
University of Dundee.
ORDERING INFORMATION
Product
Quantity Code Number
Amersham Hyperfilm ECL (5 u 7 inches)
50 sheets
28-9068-35
Amersham Hyperfilm ECL (18 u 24 cm)
Amersham Hyperfilm ECL (18 u 24 cm)
50 sheets
100 sheets
28-9068-36
28-9068-37
Amersham Hyperfilm ECL (8 u 10 inches)
50 sheets
28-9068-38
Amersham Hyperfilm ECL (8 u 10 inches)
Amersham Hyperfilm ECL (24 u 30 cm)*
100 sheets
50 sheets
28-9068-39
28-9068-40
Amersham Hyperfilm ECL (35 u 43 cm)*
50 sheets
28-9068-41
For pricing information, visit www.gelifesciences.com/orderonline
* approx 10 x 12 inches.
Related Products
Code Number
Hypertorch
RPN1620
page 393
ECL Direct Labeling and Detection System
ECL Western Blotting System
RPN3000
RPN2108
page 331
page 357
Amersham ECL Advance Western Blotting Detection Kit
ECL Western Blotting Detection Reagents
RPN2106
page 355
page 357
Amersham Hypercassette Autoradiography Cassettes
ECL Plus Western Blotting Detection Reagents
Amersham ECL DualVue Western Blotting Markers
Refer To
page 392
RPN2132
ImageQuant Imagers
page 356
page 317
page 384
Amersham Hyperfilm MP
Product
Quantity Code Number
Amersham Hyperfilm MP (5 u 7 inches)
Amersham Hyperfilm MP (18 u 24 cm)
50 sheets
50 sheets
28-9068-42
28-9068-43
Amersham Hyperfilm MP (18 u 24 cm)
Amersham Hyperfilm MP (8 u 10 inches)
100 sheets
50 sheets
28-9068-44
28-9068-45
Amersham Hyperfilm MP (8 u 10 inches)
Amersham Hyperfilm MP (24 u 30 cm)*
100 sheets
50 sheets
28-9068-46
28-9068-47
Amersham Hyperfilm MP (35 u 43 cm)*
50 sheets
28-9068-48
Amersham Hyperfilm MP Enveloped (18 u 24 cm)
50 sheets
28-9068-50
For pricing information, visit www.gelifesciences.com/orderonline
Related Products
Radiochemicals: A–Z Listing
Radioactive Labeling
Membranes for Binding Nucleic Acid
Refer To
page 93
page 332
page 324
Hyperfilm MP is also available in an envelope-sealed format.
Here, individual sheets of Hyperfilm MP are sealed in light-tight
envelopes giving maximum protection and ease of use in the life
science laboratory.
Screen
Emulsion
Antiscratch
layers
Plastic base
Emulsion
Single copy gene detection using 32P. Southern blot of
human genomic DNA, isolated from whole blood, HindIII
digest (1, 0.5, 0.25, 0.12, 0.06, 0.03 µg per lane). Detection
with n-ras insert probe (1.5Kb) labeled with 32P- dCTP.
Autoradiography with Amersham Hyperfilm MP at -70°C
with two Amersham Hyperscreens for 18 h.
radioactive emissions
light emissions
32
P/125I
Hyperfilm MP being used with an intensifying screen.
390
For more information, visit www.gelifesciences.com
GE Healthcare
PAGE: 390 - CURRENCY: USD
Hyperfilm MP is an excellent multipurpose film constructed
from a clear base coated on both sides with a photographic
emulsion that is protected by an anti-scratch layer. It may be
processed using automatic processors or in tanks and trays
with most commonly used laboratory X-ray film developers and
fixers. In a multidisciplinary laboratory in which both blots and
sequencing experiments are performed, Hyperfilm MP is the
recommended film for high performance in all applications.
ORDERING INFORMATION
PAGE: 390 - CURRENCY: USD
J New Amersham Hyperfilm MP with improved clarity.
J Coated with emulsion on both sides to ensure optimum
sensitivity.
J Multipurpose film for the high speed detection of 14C, 35S, 33P,
32P, and 125l.
J Clear base offers high contrast for easy reading of images.
J High-resolution results at an economical price
J Designed for use in diret and indirect autoradiography. The
film of choice for multipurpose work.
PAGE: 390 - CURRENCY: USD
10
J Amersham Hyperfilm ECL with improved clarity.
J High-speed detection of chemiluminescent signal from
nucleic acid and protein blots.
J System-tested with ECL and Gene Images kits to ensure
optimum performance.
J Clear base offers high contrast for easy reading of images.
J Can be processed in either automatic processors or manually
with most commonly used X-ray film developers and fixers.
PAGE: 390 - CURRENCY: USD
Gel and Blot Imaging & Analysis
Amersham Hyperfilm ECL
ch10.fm Page 391 Wednesday, June 18, 2008 4:26 PM
Autoradiography Films, Screens, and Accessories
Amersham Hyperfilm 3H
J A speciality film with a single emulsion and no anti-scratch
layer for the direct autoradiography of 3H.
J Ideal for whole body autoradiography, in situ hybridization,
and receptor localization applications.
J Single-side format ensure maximum resolution.
J Clear base offers high contrast against blackened image for
easy reading.
ORDERING INFORMATION
Product
Quantity Code Number
Hyperfilm 3H (18 u 24cm)
Refer To
Amersham Hypercoat Nuclear Emulsions
page 393
ImageQuant Imagers
page 384
emulsion
3H
Hyperfilm 3H structure
with no protective layer
allows direct detection of
3H.
Hyperfilm 3H is relatively insensitive to light and therefore not
recommended for use in fluorography or with intensifying
screens.
Amersham Hyperscreen Intensifying Screens
J Offer rapid results and high sensitivity for detecting 32P and
125I in Southern, Northern, and Western blots.
J Light emission from calcium tungstate phosphor is fully
compatible with Hyperfilm MP.
J Use with Sensitize Pre-Flash Unit for even higher sensitivity
and accurate quantitation.
J Fine grain screens give clear band resolution.
J Available in a full range of sizes to match Hypercassette and
Hyperfilm sizes.
PAGE: 391 - CURRENCY: USD
- minus Hyperscreen
+ plus Hyperscreen
++ plus Hyperscreen and preflashed with
Sensitize Preflash Unit.
ORDERING INFORMATION
Product
Quantity Code Number
Hyperscreen (18 u 24 cm)
1 pair
RPN1662
Hyperscreen (24 u 30 cm)
Hyperscreen (30 u 40 cm)
1 pair
1 pair
RPN1663
RPN1664
Hyperscreen (35 u 43 cm)
1 pair
RPN1665
Hyperscreen (18 u 43 cm)
Hyperscreen (20 u 40 cm)
1 pair
1 pair
RPN1666
RPN1667
Hyperscreen (5 u 7 inches)
Hyperscreen (8 u 10 inches)
1 pair
1 pair
RPN1668
RPN1669
Hyperscreen (10 u 12 inches)
1 pair
RPN1670
For pricing information, visit www.gelifesciences.com/orderonline
Related Products
Code Number
page 392
Autoradiography
page 389
RPN2051
10
Refer To
Amersham Hypercassette Autoradiography Cassettes
Sensitize Preflash Unit
Gel and Blot Imaging & Analysis
plastic base
Since there is no protective layer, care must be taken when
handling this film to avoid damaging the emulsion and creating
artifacts. This also means it cannot be put through automatic
processors and must be developed manually.
PAGE: 391 - CURRENCY: USD
RPN535B
Related Products
The anti-scratch layer on most films seriously impedes or totally
blocks the weak beta particles of 3H and the low energy Auger
electrons of 125I decay. As hyperfilm 3H has no anti-scratch
layer, these weak particles can strike its emulsion directly and
form an image.
PAGE: 391 - CURRENCY: USD
5 sheets
For pricing information, visit www.gelifesciences.com/orderonline
page 394
PAGE: 391 - CURRENCY: USD
Hyperscreen Intensifying Screens are economical, high
performance calcium tungstate screens for use with 32P and
125I. They can be used in conjunction with preflashed film to give
an increase in sensitivity while retaining a linear response that
allows quantitation of results.
125 I-labeled IgG slot blots: doubling dilutions
(100 nCi to 1.56 nCi) 2 h exposure to Hyperfilm MP.
GE Healthcare
For more information, visit www.gelifesciences.com
391
ch10.fm Page 392 Wednesday, June 18, 2008 4:26 PM
Autoradiography Films, Screens, and Accessories
Amersham Hypercassette Autoradiography Cassettes
ORDERING INFORMATION
Product
Quantity Code Number
1 RPN11642
Hypercassette Neutral (24 u 30 cm)
Hypercassette Neutral (35 u 43 cm)
1 RPN11643
1 RPN11645
Hypercassette Neutral (20 u 40 cm)
Hypercassette Neutral (5 u 7 inches)
1 RPN11647
1 RPN11648
Hypercassette Neutral (8 u 10 inches)
Hypercassette Neutral (10 u 12 inches)
1 RPN11649
1 RPN11650
Hypercassette Neutral (deep depth)
Hypercassette Neutral (18 u 24 cm)
Hypercassette Neutral (30 u 40 cm)
1 RPN11628
1 RPN11627
Hypercassette Neutral (8 u 10 inches)
1 RPN11629
Hypercassette Red (standard depth)
Hypercassette Autoradiography Cassettes.
J Robust and chip-proof autoradiography cassettes for
light-tight exposures at temperatures as low as -70°C.
J Maximum contact between film and sample.
J Nonporous pad gives a wipe clean surface ensuring
minimum contamination.
1 RPN12642
Hypercassette Red (24 u 30 cm)
Hypercassette Red (35 u 43 cm)
1 RPN12643
1 RPN12645
Hypercassette Red (8 u 10 inches)
1 RPN12649
Hypercassette Blue (standard depth)
Hypercassette Blue (18 u 24 cm)
Hypercassette Blue (24 u 30 cm)
1 RPN13642
1 RPN13643
Hypercassette Blue (35 u 43 cm)
1 RPN13645
Hypercassette Blue (8 u 10 inches)
1 RPN13649
For pricing information, visit www.gelifesciences.com/orderonline
Related Products
Refer To
Amersham Hyperscreen Intensifying Screens
page 391
Autoradiography
page 389
Amersham Amplify Fluorographic Reagent
Amplify fluorographic reagent can increase sensitivity of
detection and significantly reduce exposure times required for
weak beta emitters.
ORDERING INFORMATION
Product
Quantity Code Number
Amplify Fluorographic Reagent
1 liter
NAMP100
For pricing information, visit www.gelifesciences.com/orderonline
Related Products
Code Number
Amersham Hyperfilm MP
Refer To
page 390
Sensitize Preflash Unit
[14C] Protein Molecular Weight Markers
RPN2051
Tritiated Amino Acids
page 394
page 319
page 91
emulsion
plastic base
emulsion
anti-scratch
layer
3H/Auger
gel
plastic base
anti-scratch
layer
Autoradiographs showing the detection of 35S- and 3Hlabeled proteins in acrylamide gels with (+) and without
(-) Amplify. Samples exposed to preflashed Hyperfilm
MP.
392
3H/Auger
For more information, visit www.gelifesciences.com
gel
Internal absorption
of weak b-emissions
within a sample.
Gel impregnated
with fluorographic
reagent such as
Amplify. Weak bemissions are
converted to light
and efficiently
recorded on film.
GE Healthcare
PAGE: 392 - CURRENCY: USD
J Fast and convenient fluorographic reagent for 35S-, 14Cand 3H-labeled samples in gels.
J Increased detection efficiency 1000-fold for 3H and 15-fold
for 35S and 14C.
J Less than two hours total preparation time.
J Can be used with acrylamide or agarose gels with no change
in protocols.
J Odorless, inorganic and fully compatible with Sensitize
Preflash unit and Hyperfilm MP.
PAGE: 392 - CURRENCY: USD
Hypercassette autoradiography cassettes are designed for the
toughest demands of life science research. Deep cassettes are
1.5 cm in depth, allowing them to accommodate thicker
samples and a choice of Hypercassette colors enables color
coding of work. Record details on the polycarbonate label and
wipe clean after use.
Hypercassette Red (18 u 24 cm)
PAGE: 392 - CURRENCY: USD
10
Hypercassette Neutral (18 u 24 cm)
PAGE: 392 - CURRENCY: USD
Gel and Blot Imaging & Analysis
Hypercassette Neutral (standard depth)
ch10.fm Page 393 Wednesday, June 18, 2008 4:26 PM
Autoradiography Films, Screens, and Accessories
Amersham Hypertorch
ORDERING INFORMATION
Product
Quantity Code Number
Hypertorch (includes 2 AAA batteries)
Hypertorch—battery-powered, darkroom pen torch.
Related Products
Refer To
Autoradiography
page 389
Amersham Hypercassette Autoradiography Cassettes
page 392
Hypertorch contains a red LED which produces a safe
wavelength of light for short exposures to film.
Amersham Hypercoat Nuclear Emulsions
ORDERING INFORMATION
Quantity Code Number
Hypercoat LM-1
2 u 20 ml
RPN40
Hypercoat EM-1
2 u 20 ml
RPN41
For pricing information, visit www.gelifesciences.com/orderonline
PAGE: 393 - CURRENCY: USD
PAGE: 393 - CURRENCY: USD
PAGE: 393 - CURRENCY: USD
PAGE: 393 - CURRENCY: USD
Product
Related Products
Code Number
Hypertorch
Amersham Hyperfilm 3H
RPN1620
Refer To
this page
page 391
Hypercoat nuclear emulsions and Hypercoat dipping vessel.
J Ready-to-use nuclear emulsions that can be used in the
microautoradiography of radiolabels in tissues or cells.
J Uniform crystal size and aggregate-free emulsions ensure
high resolution.
J Use Hypercoat LM-1 (0.2 µm crystal size) for general
microautoradiography with 14C, 35S, 33P, 32P, 3H, and 125I.
J Use Hypercoat EM-1 (0.13 µm crystal size) for high-resolution
light or electron microscopy with 3H-labeled samples.
J High silver content allows reduced exposure times.
Binding of [125I]endothelin-I to artery
wall of patient with ischaemic heart
disease, visualized with Hypercoat LM-I.
Gel and Blot Imaging & Analysis
J Red light darkroom torch for localized illumination.
J Minimizes film backgrounds by only illuminating local areas
of work in a darkroom.
J Allows visibility in areas of darkroom where fixed safelights
cannot reach.
3 RPN1620
For pricing information, visit www.gelifesciences.com/orderonline
10
Hypercoat EM-1 is recommended for high-resolution results
when working with 3H. Isotopes such as 14C, 35S, 33P, 32P, and
125I are best detected with Hypercoat LM-1 which has a slightly
larger grain size optimized for use with higher energy emissions.
Both emulsions come ready to use and do not require diluting,
a process that can increase backgrounds.
Amersham Microscale Autoradiography Standards
For main product entry, see page 118.
Amersham Liquid Scintillation Cocktails
For main product entry, see page 117.
GE Healthcare
For more information, visit www.gelifesciences.com
393
ch10.fm Page 394 Wednesday, June 18, 2008 4:26 PM
Autoradiography Films, Screens, and Accessories
Amersham Sensitize Preflash Unit
ORDERING INFORMATION
Product
Quantity Code Number
Sensitize Preflash Unit
1 RPN2051
(Batteries not included)
Related Products
Code Number
ECL Western Blotting System
ECL Western Blotting Detection Reagents
RPN2108
RPN2106
Refer To
page 357
page 357
Amersham Hyperfilm ECL
Amersham Hyperfilm MP
Lambda HindIII digests
(10-fold dilutions)
detected with ECL Direct
and exposed to
Hyperfilm ECL. Note the
presence of a 0.56 kb
fragment after
preflashing.
Amersham TrackerTape
Product
Quantity Code Number
TrackerTape (10 u 15 cm)
10 sheets
RPN2050
For pricing information, visit www.gelifesciences.com/orderonline
These products are not available in all countries. Please contact your local office for
further details.
10
Refer To
Autoradiography
page 389
J A phosphorescent tape which, when written on, can be used
to label samples and corresponding autoradiographs.
J Produces a permanent negative image in minutes.
J Allows samples to be matched to results.
J Ordinary marker pen is used to write on the tape.
Western blot identified by TrackerTape.
394
For more information, visit www.gelifesciences.com
GE Healthcare
PAGE: 394 - CURRENCY: USD
Related Products
PAGE: 394 - CURRENCY: USD
ORDERING INFORMATION
PAGE: 394 - CURRENCY: USD
J Specially designed flashgun for use with Hyperfilm ECL or
Hyperfilm MP in conjunction with Hyperscreen Intensifying
Screens to improve sensitivity and quantitation.
J Most effective when detecting small amounts of radioactivity
or chemiluminescent signal.
J High quality orange filter included to ensure optimum
wavelength for preflashing.
J Sealed unit to prevent white-light leakage.
J Calibration protocol included to ensure reproducibility.
page 390
page 390
PAGE: 394 - CURRENCY: USD
Gel and Blot Imaging & Analysis
For pricing information, visit www.gelifesciences.com/orderonline
ch10.fm Page 395 Wednesday, June 18, 2008 4:26 PM
Autoradiography Films, Screens, and Accessories
Amersham Hyperprocessor Automatic Film Processor
ORDERING INFORMATION
Product
Quantity Code Number
Hyperprocessor 220/240 V
1 RPN1700
Hyperprocessor 110/120 V
1 RPN1700A
Includes: Trolley, Two Chemical Reservoirs, Small Spares Kit, Full Operator Manual and Full
Installation Manual.
For pricing information, visit www.gelifesciences.com/orderonline
PAGE: 395 - CURRENCY: USD
Refer To
Autoradiography
page 389
Amersham Hypercassette Autoradiography Cassettes
page 392
Hyperprocessor is designed to give consistent high-quality
autoradiographs simply and quickly with Hyperfilm MP,
Hyperfilm ECL, Kodak BioMax, X-Omat AR, X-Omat LS, and most
standard X-ray films. Sensors measure the film throughput and
replenish chemicals as needed. Chemicals are maintained at
optimum temperatures for quality results.
TECHNICAL SPECIFICATIONS
Safety certifications
PAGE: 395 - CURRENCY: USD
J An advanced automatic film processor of compact bench
top design, requiring less than 0.2 m2 of floor space.
J Develops, fixes, washes, and dries film automatically in 2.5
min.
J Internal control systems ensure consistent high performance.
J Handles film sizes from 10 u 10 cm to 35 u 43 cm.
J Can process 70 films, size 24 u 30 cm, per h.
Related Products
CE 89/336/EEC (EMC directive);
CE 73/23/EEC (LV directive);
EN-61010-1 (IEC1010-1)
Gel and Blot Imaging & Analysis
Hyperprocessor is not available in all countries. Please contact your local office for
further details.
PAGE: 395 - CURRENCY: USD
PAGE: 395 - CURRENCY: USD
10
GE Healthcare
For more information, visit www.gelifesciences.com
395
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