Typhoon™ FLA 9000 biomolecular imager

Typhoon™ FLA 9000 biomolecular imager
GE Healthcare
Data file 28-9610-72 AA
Imaging systems, software, and accessories
Typhoon™ FLA 9000 biomolecular
imager
Typhoon FLA 9000 (Fig 1) is a versatile laser scanner for
biomolecular imaging applications, including sensitive
and quantitative measurement of radioisotopic labels,
chemifluorescent Western blots, 2-D DIGE, multiplex
fluorescence (visible and near infrared excitation) and
colorimetric stains (e.g., Coomassie™ blue and silver-stained
gels). Typhoon FLA 9000 offers high performance for highly
sensitive detection and accurate quantitation of proteins
afforded by chemifluorescent Amersham™ ECL™ Plus and
multifluorescent ECL Plex™ Western blotting systems.
Typhoon FLA 9000 delivers:
• Versatility: image radioisotope-, multifluorescent-,
chemifluorescent-, colorimetric-, and chemiluminescentlabeled samples at any time
Fig 1. Typhoon FLA 9000 is a high performance biomolecular imager, with a
laser scanner for sensitive and quantitative measurements.
Typhoon FLA 9000 is a variable mode laser scanner with
modular access to the optical components and excitation
sources, providing both versatile and flexible imaging for
precise quantitation of proteins, nucleic acids, and other
biomolecules.
• High resolution and quantitation: a pixel resolution of
up to 10 µm and a linear signal response over five orders
of magnitude provides precise quantitation in gels, blots,
tissue sections, and arrays
• Flexibility: optimize performance for new applications by
adapting the system with stages, detectors, filters, and
lasers
• High sample throughput: simultaneously image up
to 16 blots or gels measuring 10 × 8 cm, facilitating
comparisons among blots, and reducing workload and
waiting time
• 2-D DIGE imaging: simultaneously image two 2-D DIGE
gels for differential expression studies
• Visible and infrared fluorescence imaging: optional near
infrared excitation for imaging IRDye™ and other infrared
dyes
Fig 2. Filters are easily exchanged by the user.
The system provides several imaging modes at any time:
fluorescence and chemifluorescence, radioisotopic imaging,
and digitization of colorimetrically-stained samples (e.g.,
Coomassie blue and silver stain). The system is also adequate
for quantitative chemiluminescent imaging that does not
require maximum sensitivity. For detection of low abundance
proteins by chemiluminescence, the ImageQuant™ imager
series is recommended.
CHO cell lysate
5000
2500
1250
630
310
160
78
(ng)
Sample:
CHO cell lysate
Membrane:
Hybond™ LFP
Target protein: β-tubulin (Cy5, red)
ERK 1/2 (Cy3, green)
Detection:
Primary antibodies:
mouse anti-tubulin and rabbit anti-MAP kinase ERK1/2
Secondary antibodies:
ECL Plex anti-rabbit Cy3 and ECL Plex anti-mouse Cy™5
Imaging:
Excitation Emission filter
Cy3:
532 nm
BPG1 (570DF20)
Cy5:
635 nm
LPR (665LP)
LOD:
β-tubulin in 160 – 78 ng CHO cell lysate (Cy5)
ERK 1/2 in 78 ng CHO cell lysate (Cy3)
Fig 3. Multiplex detection of endogenous proteins by ECL Plex Western
blotting. Tubulin and ERK1/2 were targeted in a dilution series of CHO cell
lysate by using mouse anti-tubulin and rabbit anti-MAP kinase ERK 1/2
primary antibodies. Secondary antibodies were ECL Plex anti-mouse Cy™5
(red) and anti-rabbit Cy3 (green). Imaging was performed with Typhoon
FLA 9000 using separate detection channels. Arrows indicate the limits of
detection (LOD) in each detection channel. This figure shows no crosstalk
and low background, which affords reliable quantitation relative to a
housekeeping protein.
The system is optimized for: 2-D DIGE imaging for differential
protein expression studies, ECL Plus chemifluorescent
imaging for quantitative protein detection by Western
blotting, and multifluorescent ECL Plex imaging for precise
quantitation of two or more proteins in the same blot (Fig 3).
Table 1. Typhoon FLA 9000 specifications
Detection modes:
Fluorescence, phosphorimaging, digitization, and chemiluminescence
Excitation wavelengths:
473 nm (blue LD laser),
532 nm (green SHG laser),
635 nm (red LD laser),
685 nm (optional red LD laser), and 785 nm (optional near IR LD laser)
Radioisotopes:
3
Dynamic range:
5 orders of magnitude
Bit depth:
16-bit
Scanning area:
40 × 46 cm
Pixel sizes: 10, 25, 50, 100, 200 µm and prescan 1000 µm
Standard filters:
IP (Phosphorimaging),
LPB (510LP), LPG (575LP), LPR (665LP), BPB1 (530DF20), and BPG1 (570DF20)
Optional filters:
BPFR700 (R715), BPFR800 (R810),
DBR1 (530DF20/665LP),
and DGR1 (570DF20/665LP)
Dimensions (W × H ×D):
900 × 800 × 400 mm
Weight:
97 kg
Line frequency:
50/60 Hz
Temperature:
15°C to 30°C
Humidity:
20% to 70% (no condensation)
Supply voltage:
100 - 240 VAC ± 10%
Power consumption:
Approx. 0.3 kVA
H, 14C, 32P, 33P, 35S, 125I
Technical features
Optimal choice of filter, stage, laser, and PMT
A
B
C
D
Fig 4. (A) The Phosphor Stage, (B) Multi Stage, (C) Low Fluorescent Glass Plate
Stage, and (D) Fluor Stage are designed to accommodate a variety of sample
formats and imaging modes.
2
10/2009 28-9610-72 AA
Filters are easily accessed and exchanged without tools
to attain optimal imaging conditions (Fig 2). The system
accommodates up to four computer-controlled filter
positions at any time. Custom filters can be easily installed
by the user.
Stages (Fig 4) give the correct positioning and stability for
optimal imaging of a range of samples. Samples that can
be scanned include agarose and polyacrylamide gels,
membranes, DIGE gels, radioisotope-labeled samples, as
well as microplates and glass slides with the TP plug-in.
The system can simultaneously scan two DIGE gels, each
measuring up to 21.5 x 27.5 cm with the Low Fluorescent
Glass Plate stage. The stages are easily removed from the
system for cleaning.
Transferrin
5000
2500
1250
630
310
160
78
39
19.5 (pg)
Typhoon FLA 9000
2500
1250
Transferrin
630
310
Sample:
Transferrin
Membrane: Hybond LFP
Detection:
Primary antibody:
rabbit anti-human transferrin
Secondary antibody:
anti-rabbit IRDye 680
Imaging: Excitation Emission filter
Typhoon
FLA 9000: 685 nm
BPFR700 (R715)
LOD:
19.5 pg
DR:
2.1 orders of magnitude
0.9904
R2:
160
78
35000
30000
25000
20000
15000
10000
5000
0
2500
1
2
3
4
5
6
amount of transferrin (ng)
Transferrin
1250
630
310
160
78
39
19.5 (ng)
40000
30000
20000
10000
0
0
1
2
3
4
transferrin (ng)
5
6
Fig 5. A two-fold dilution series of transferrin starting at 5 ng was
subjected to Western blotting and detected with a rabbit anti-transferrin
primary antibody and anti-rabbit Alexa 633 secondary antibody. Results
demonstrate detection in the near infrared wavelength region with a linear
signal response. Arrow indicates the limit of detection (LOD).
Sample:
Transferrin
Membrane: Hybond LFP
Detection:
Primary antibody:
rabbit anti-human transferrin
Secondary antibody:
anti-rabbit IRDye 680
Imaging: Excitation Emission filter
Odyssey:
685 nm
700
LOD:
19.5 pg
DR:
2.1 orders of magnitude
0.9804
R2 :
45000
Integrated Intensity (× 103)
Integrated Intensity (× 103)
Odyssey™
50000
19.5 (ng)
40000
0
Sample:
Transferrin
Membrane: Hybond LFP
Detection:
Primary antibody:
rabbit anti-human transferrin
Secondary antibody:
anti-rabbit Alexa 633
Imaging: Excitation Emission filter
Alexa 633: 635 nm
LPR (665LP)
LOD:
39 pg transferrin
DR:
2.1
R2 :
0.9947
39
45000
Integrated Intensity (× 103)
The system can be equipped for dual simultaneous
fluorescence detection by the addition of a second
photomultiplier tube (PMT). Each PMT is selected for optimal
response to the detected emission wavelength. The standard
bialkali PMT1 is optimal for phosphorimaging and dyes
excited by blue (e.g., Cy2), green (e.g., Cy3) and red (e.g.,
Cy5) light, whereas the optional multialkali PMT2 is optimal
for dyes excited by red and infrared light (e.g., IRDye680,
IRDye800, and Alexa Fluor 633; see Figs 5 and 6).
40000
35000
30000
25000
20000
15000
10000
5000
0
0
Scanning is rapid and detection is sensitive for laser-induced
fluorescence, radioisotopic imaging by storage phosphor, and
digitization. A fast 1000 µm prescan function gives a rapid
overview of the sample for selecting the scan area.
At a pixel size of 200 µm, a 10 × 15 cm sample is scanned in
two minutes. The system provides a linear signal response
over five orders of magnitude. This, together with digitization
of the image with up to 16-bit resolution, provides a suitable
basis for the precise quantitation of proteins, DNA, and other
suitably labeled molecules in complex samples.
Lasers can be exchanged in the field to accommodate new
applications and fluorophores. The system can house up
to four lasers simultaneously, from a choice of five laser
excitation wavelengths (473, 532, 635, 685, and 785 nm).
The system employs cost-effective and space-saving diode
lasers.
1
2
3
4
5
6
amount of transferrin (ng)
Fig 6. A two-fold dilution series of transferrin starting at 2.5 ng was
subjected to Western blotting and detected with a rabbit anti-transferrin
primary antibody and anti-rabbit IR Dye 680 secondary antibody. Imaging
by Typhoon FLA 9000 showed similar performance for detection in the near
infrared wavelength region compared to the Li-Cor™ Odyssey scanner.
Arrows indicate the LOD. The experiments were performed at GE Healthcare
laboratories according to the manufacturers’ instructions..
Optimal detection of chemifluorescent
Western blots
Laser scanning systems are not optimal for imaging
chemiluminescence. Typhoon FLA 9000 does, however,
perform good with Amersham ECL Plus by imaging its stable
chemifluorescent signal, which is emitted by ECL Plus upon
excitation by the 473 nm laser. This provides a means for you
to obtain optimal imaging performance from a dual mode
chemiluminescent and chemifluorescent reagent.
10/2009 28-9610-72 AA
3
Ettan™ DIGE system
Ettan DIGE system is an integrated solution for accurate
quantitation of changes in protein expression. Typhoon FLA
9000 is a fully optimized part of Ettan DIGE system with
DeCyder™ 2D Differential Analysis Software (Fig 7).
The strengths of Typhoon FLA 9000—high sensitivity and
broad dynamic range for measuring low and high abundant
proteins in one scan (Fig 8 )—makes it highly suited for
2-D DIGE applications, enabling the user to visualize and
accurately quantitate subtle changes in protein expression.
By generating overlaid, multichannel images for each
gel, Typhoon FLA 9000 exploits the multiplexing potential
of CyDye™ DIGE Fluors to reduce experimental variation
among samples. Images are analyzed using DeCyder 2D to
accurately measure very small protein differences with
a high degree of confidence.
Light measurement
Detection of radioactivity
Samples containing radioactive probes are exposed to
a storage phosphor screen. Light is emitted from the
storage phosphor screen in proportion to the amount of
radioactivity in the sample upon laser-induced stimulation.
Emitted light is collected and converted to an electrical
signal by a photomultiplier (PMT). The electrical signal is
digitized for image display and analysis.
Fluorescence
Upon excitation, light is emitted from a fluorescently-labeled
sample in proportion to the amount of labeled compound
in the sample. Emitted light is collected and converted
to an electrical signal by a PMT. The electrical signal is
digitized for image display and analysis. Multiple fluorescent
wavelengths can be detected with minimal crosstalk for
comparative expression experiments. See Table 3 for
emission filters.
Sample:
CHO cell lysate, expressing
MAb grown at various
culture conditions
Gel:
large precast DIGE Gel
(within glass cassette)
Imaging: Excitation Emission filter
Cy2:
473 nm
BPB1 (530DF30)
Cy3:
532 nm
BPG1 (570DF20)
Cy5:
635 nm
LPR (665LP)
Fig 7. CHO cells, expressing MAb, were grown in different culture media
compositions. 2D-DIGE was used to analyze host cell proteins to improve
MAb process understanding. Gels were imaged using Typhoon FLA 9000
and Typhoon 9410 scanners. Image analysis was performed using DeCyder
2D software, where statistical analysis revealed similar results from both
scanners.
Carbonic anhydrase
409.6 204.8 102.4 51.2
25.6
12.8
6.4
3.2
1.6
0.8
0.4
0.2 (ng)
Upon excitation, emitted light from an enzyme-catalyzed
fluorescent product is collected and converted to an
electrical signal by a PMT. The electrical signal is digitized
for image display and analysis.
Digitization
Light passes through the sample twice. Once transmitted
through the sample, fluorescence is emitted from a
fluorescent plate. The fluorescent light is attenuated on
the return path through the sample. The light is collected
and converted to an electrical signal by a PMT. The
electrical signal is digitized for image display. The resultant
image is non-quantitative, and is therefore suitable for
documentation purposes only.
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10/2009 28-9610-72 AA
Sample:
Label:
Carbonic anhydrase
CyDye DIGE fluor,
minimal dye Cy3
Gel:
12% acrylamide
Tris-glycine
Imaging: Excitation Emission filter
Cy3:
532 nm
BPG1 (570DF20)
LOD:
0.2 ng carbonic anhydrase
DR:
3.3 orders of magnitude
0.9981
R2:
Integrated Intensity (× 103)
Chemifluorescence
70000
60000
50000
40000
30000
20000
10000
0
0
100 200 300 400 500
amount of carbonic anhydrase (ng)
Fig 8. Different concentrations of carbonic anhydrase were labeled with
CyDye DIGE fluor minimal Cy3 and subjected to 1-D electrophoresis.
The gel was imaged with Typhoon FLA 9000. The detection limit (LOD)
was 0.2 ng carbonic anhydrase and the linear dynamic range (DR) was
3.3 orders of magnitude, indicating an excellent basis for quantitation.
Arrow indicates the LOD.
Chemiluminescence
The light detection path is scanned across the sample
without illumination. The emitted chemiluminescence
from each scanned point is collected and converted to an
electrical signal by a PMT. The electrical signal is digitized
for image display and analysis.
Data storage
Data are stored either in linear 16-bit grayscale TIFF (.TIF
file format) or in square root encoded 16-bit TIFF (.GEL file
format). The .GEL format encoding provides higher dynamic
resolution than .TIF at lower signal levels to exploit the
low signal detection capability of the phosphorimaging
technology.
Image analysis
Designed for quantitative gel, blot, and colony analysis and
seamless data transfer, we provide image analysis software
for use with Typhoon (Table 2).
Table 2. Image analysis software
Software Analysis
ImageQuant TL 1-D gel electrophoresis, dot blots,
arrays, colony counting, 2-D spot
measurement, and user-defined gel analysis
DeCyder 2D
Differential high-resolution
2-D DIGE analysis including
Extended Data Analysis
Table 3. Emission filters
Filter type
Wavelength
range (nm)
IP
- Phosphorimaging
LPB (510LP)
≥ 510
BPB1 (530DF30) 515 – 545
LPG (575LP)
≥ 575
BPG1 (570DF20) 560 – 580
Fluorochrome
examples
Cy2 DIGE Fluor,
ECL Plex Cy2
Cy2, ECL Plus,
SYBR™ Green, FAM™,
FITC, Alexa™ Fluor
488, SYPRO™ Ruby,
SYPRO Orange, GFP
Cy3, Deep Purple™, HEX,
Alexa Fluor 532 & 555,
SYPRO Red
Cy3 DIGE Fluor,
ECL Plex Cy3
LPR (665 LP)
≥ 665
Cy5, Alexa Fluor 633,
TOTO™ 3, DiD,
Cy5 DIGE Fluor,
ECL Plex Cy5
BPFR700 (R715) 713 – 726
Alexa Fluor 700,
IRDye680,
IRDye700
BPFR800 (R810)
814 – 826
Alexa Fluor 790,
IRDye800
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5
Imager performance
Typhoon
9400/9410
Typhoon
FLA 9000
Typhoon Typhoon
Trio/Trio+ FLA 7000
Storage Phosphor
32
P, 125I, 14C, 35S, 33P, 3H Macroarray (radiolabeled) ++++ ++++ ++++
++++ ++++ ++++ ++++
—
Fluorescence—Proteins
CyDye DIGE Fluors
Cy2 Cy3 Cy5 ++++ ++++ ++++ +++ ++++ ++++ ++++ ++++ ++++ —*
—*
—*
ECL Plex Fluors
Cy2 Cy3 Cy5 ++++ ++++ ++++ +++ ++++ ++++ ++++ ++++ ++++ —*
—*
—*
Protein Stains
Deep Purple Total Protein Stain
SYPRO Ruby
NanoOrange™ (solutions) Pro-Q Diamond
(phosphorylated proteins) Pro-Q Sapphire 532
(Histidine-tagged proteins) ++++
++++
++++
++++
+++ ++++
++++
++++
+++
+++ ++++ +++ ++++ ++
++++ +++ ++++ ++
ELISA
AttoPhos
Fluorescence—Nucleic acids
Cy3 and Cy5 Alexa Fluor 532 and
Alexa Fluor 633 ++++
+++
++++ ++++ ++++ +++
++++ ++++ ++++ +++
Nucleic acid stains
Ethidium Bromide (post stain) Vistra Green, SYBR Gold,
SYBR Green I and II ++++ +++ ++++ +++
++++ ++++ +++ +++
PicoGreen, RiboGreen
+++
+++
+++ ++++
++++
++
++
+++
Chemifluoresence (enzyme-catalyzed) ECL Plus Western blotting
++++
+++
ECF, AlkPhos direct ECF
++++
+++
DDAO Phosphate
++++
++++
Multipurpose fluorescence
Cy2 Cy3 Cy5 Fluorescein, FAM, FITC,
Alexa Fluor 488 TET, HEX, ROX, TAMRA ++++ ++++ ++++ +++ ++++ ++++ ++++ ++++ ++++ ++
+++
+++
++++ ++++ ++++ +++ ++++ ++++ +++
++
Other applications
GFP
+++
+++
+++
++
Chemiluminescence +
+
+
—
ECL
ECL Plus
ECL Advance
++++ Superior performance +++ High performance ++ Good performance + Acceptable performance — Not compatible
Ratings are based on overall system performance including model-specific features, versatility, and sensitivity (limit of detection). Blank fields indicate that data are not available.
* Multiplex experiments (e.g., 2-D DIGE and ECL Plex) cannot be performed on Typhoon FLA 7000. CyDye DIGE Fluors and ECL Plex conjugates can be imaged in single probe experiments
on Typhoon FLA 7000 (i.e., experiments where there is only one dye or conjugate on the gel or membrane).
6
10/2009 28-9610-72 AA
Ordering information
System
Typhoon FLA 9000 *
Quantity Code no.
1
28-9558-08
*Includes 473 nm, 532 nm, and 635 nm lasers, filter tray, IP filter, LPB filter, LPG filter, LPR filter,
BPB1 filter, BPG1 filter, Fluor Stage, Membrane Weight, Phosphor Stage, Low Fluorescent
Glass Plate Stage, Multi Stage, and TP plug-in. Fluorescent plate for digitization, capture
software, USB cable, mains cables (EU and USA), User Manual, Getting Started Guide.
Upgrades and accessories Quantity Code no.
Fluor Stage Set
1
28-9589-04
1
28-9564-19
1
28-9564-74
1
28-9564-75
1
28-9564-76
1
28-9564-77
1
28-9564-78
1
28-9564-81
1
28-9564-82
Fluor Stage, Membrane Weight
Multi Stage Set
Multi Stage, TP plug-in
BAS-IP MS 2040 E
Water-resistant phosphorimaging plate,
20 × 40 cm, multipurpose
BAS-IP MS 2025 E
Water-resistant phosphorimaging plate,
20 × 25 cm, multipurpose
BAS-IP MS 3543 E
Water-resistant phosphorimaging plate,
35 × 43 cm, multipurpose
BAS-IP SR 2040 E
Water-resistant phosphorimaging plate,
20 × 40 cm, high resolution
BAS-IP SR 2025 E
Water-resistant phosphorimaging plate,
20 × 25 cm, high resolution
BAS-IP TR 2040 E
Phosphorimaging plate, 20 × 40 cm,
for Tritium detection
BAS-IP TR 2025 E
Phosphorimaging plate, 20 × 25 cm,
for Tritium detection
FLA Image Eraser
1
28-9564-73
685 nm laser upgrade
1
28-9610-22
1
28-9610-25
1
28-9610-32
685 nm laser, BPFR700 filter, PMT2,
installation, and testing
785 nm laser upgrade
785 nm laser, BPFR800 filter, PMT2,
installation, and testing
685 + 785 nm laser upgrade
685 nm and 785 nm lasers, BPFR700 filter,
PMT2, BPFR800 filter, installation, and testing
Related literature Typhoon FLA 7000 biomolecular imager, Data file
Code no.
28-9610-73
Minimum computer requirement
OS: Windows™ XP™ SP3 (32-bit) or Windows Vista™ Business SP1
(32-bit), RAM: more than 1 GB, Processor: Intel™ Core 2 Duo
processors, Hard disk: more than 80 GB, USB Ports: USB 2.0,
Optical drive: DVD-ROM or Super Multi Drive,
Monitor: 1280 × 1024 pixel resolution or higher
Please contact your local sales representative for the latest recommended computer
configuration.
10/2009 28-9610-72 AA
7
For local office contact information, visit
www.gelifesciences.com/contact
www.gelifesciences.com/quantitative_imaging
GE Healthcare Bio-Sciences AB
Björkgatan 30
751 84 Uppsala
GE, imagination at work, and GE monogram are trademarks of General Electric Company.
Amersham, Cy, CyDye, DeCyder, Deep Purple, ECL, ECL Advance, ECL Plex, Ettan, Hybond, ImageQuant,
and Typhoon are trademarks of GE Healthcare companies.
2-D DIGE: 2-D Fluorescence Difference Gel Electrophoresis (2-D DIGE) technology is covered by US patent
numbers 6,043,025, 6,127,134 and 6,426,190 and equivalent patents and patent applications in other
countries and exclusively licensed from Carnegie Mellon University.
DeCyder: This release of DeCyder version 2 (software) is provided by GE Healthcare to the customer
under a non-exclusive license and is subject to terms and conditions set out in the 2-D Differential Gel
Electrophoresis Technology Access Agreement. Customer has no rights to copy or duplicate or amend
the Software without the prior written approval of GE Healthcare.
Deep Purple Total Protein Stain: Deep Purple Total Protein Stain is exclusively licensed to GE Healthcare
from Fluorotechnics Pty Ltd. Deep Purple Total Protein Stain may only be used for applications in life science
research. Deep Purple is covered under a granted patent in New Zealand entitled “Fluorescent Compounds”,
patent number 522291 and equivalent patents and patent applications in other countries.
ECL Advance: ECL Advance contains Lumigen TMA-6 substrate and is sold under exclusive license from
Lumigen Inc.
ECL Plus contains Lumigen PS3 substrate and is sold under exclusive license from Lumigen Inc.
CyDye: This product or portions thereof is manufactured under an exclusive license from Carnegie Mellon
University under US patent number 5,268,486 and equivalent patents in the US and other countries.
Cy3-UTP or Cy5-UTP, Cy3. 5-dCTP or Cy5.5-dCTP, Cy3-CTP or Cy5-CTP: These products are manufactured
for GE Healthcare UK Limited by Perkin Elmer Life Sciences under US patent numbers 5047519 and 5151507.
The cyanine dyes in the product are manufactured under an exclusive license from Carnegie Mellon
University under US patent numbers 5,268,486 and equivalent patents in the US and other countries.
The purchase of CyDye products includes a limited license to use the CyDye products for internal research
and development but not for any commercial purposes.
A license to use the CyDye products for commercial purposes is subject to a separate license agreement
with GE Healthcare. Commercial use shall include:
1. Sale, lease, license or other transfer of the material or any material derived or produced from it.
2. Sale, lease, license or other grant of rights to use this material or any material derived or produced from it.
3. Use of this material to perform services for a fee for third parties, including contract research and drug
screening.
If you require a commercial license to use this material and do not have one, return this material unopened
to GE Healthcare Bio-Sciences AB, Bjorkgatan 30, SE-751 84 Uppsala, Sweden and any money paid for the
material will be refunded.
All third party trademarks are the property of their respective owners.
©2009 General Electric Company—All rights reserved.
First published Oct. 2009.
All goods and services are sold subject to the terms and conditions of sale of the company within
GE Healthcare which supplies them. A copy of these terms and conditions is available on request.
Contact your local GE Healthcare representative for the most current information.
GE Healthcare UK Limited
Amersham Place
Little Chalfont
Buckinghamshire, HP7 9NA
UK
GE Healthcare Europe, GmbH
Munzinger Strasse 5
D-79111 Freiburg
Germany
GE Healthcare Bio-Sciences Corp.
800 Centennial Avenue, P.O. Box 1327
Piscataway, NJ 08855-1327
USA
GE Healthcare Bio-Sciences KK
Sanken Bldg., 3-25-1, Hyakunincho
Shinjuku-ku, Tokyo 169-0073
Japan
28-9610-72 AA 10/2009
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