QIAGEN RNeasy Lipid Tissue Mini Kit, Lipid Tissue Midi Kit RNA purification kit Handbook

Below you will find brief information for RNeasy Lipid Tissue Mini Kit, RNeasy Lipid Tissue Midi Kit. These kits are for the optimal lysis of tissues rich in fat, such as brain and adipose tissue, and purification of high-quality total RNA. They are also compatible with all other types of animal tissue. The kits effectively remove most DNA without DNase treatment, but optional on-column DNase digestion is available for sensitive applications.

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QIAGEN RNeasy Lipid Tissue Mini Kit, RNeasy Lipid Tissue Midi Kit Handbook | Manualzz

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Key features

  • Efficient lysis of fatty tissues
  • Purification of high-quality total RNA
  • Compatible with all animal tissues
  • Includes QIAzol Lysis Reagent for RNase inhibition
  • RNeasy spin columns for RNA binding and purification
  • Optional on-column DNase digestion
  • High RNA yields and purity

Frequently asked questions

The RNeasy Lipid Tissue Kits are specifically designed for fatty tissues like brain and adipose tissue, but they can also be used for all other types of animal tissue.

For brain or adipose tissue, you can process a maximum of 100 mg. For other tissues, the maximum is generally 50 mg.

Typically, DNase digestion is not required as the kits effectively remove most DNA. However, optional on-column DNase digestion is available for applications sensitive to very small amounts of DNA.

To ensure RNA stability, you can either immediately freeze tissues in liquid nitrogen and store at -70°C, or immediately immerse them in RNAlater RNA Stabilization Reagent at room temperature.
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