MSQ Plus Mass Detector Getting Started Guide

MSQ Plus Mass Detector Getting Started Guide
MSQ Plus Mass Detector
Getting Started Guide
60111-97044 Revision A
September 2013
© 2013 Thermo Fisher Scientific Inc. All rights reserved.
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Release history: Revision A September 2013
Software version: (Thermo) MSQ Plus Mass Detector 2.0 or later; Xcalibur 2.2 SP1 or later; Foundation 2.0
SP1, 2.1, 3.0 or later
For Research Use Only. Not for use in diagnostic procedures.
Regulatory Compliance
Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with
applicable domestic and international regulations. When the system is delivered to you, it meets all pertinent
electromagnetic compatibility (EMC) and safety standards as described in the next section or sections by product name.
Changes that you make to your system may void compliance with one or more of these EMC and safety standards.
Changes to your system include replacing a part or adding components, options, or peripherals not specifically
authorized and qualified by Thermo Fisher Scientific. To ensure continued compliance with EMC and safety standards,
replacement parts and additional components, options, and peripherals must be ordered from Thermo Fisher Scientific
or one of its authorized representatives.
EMC Directive 89/336/EEC as amended by 92/31/EEC and 93/68/EEC
EMC compliance has been evaluated by UNDERWRITERS LABORATORY, INC (UL).
EN 55011
(1998)
EN 61000-4-3
(2002)
EN 61326-1
(1998)
EN 61000-4-4
(2001)
EN 61000-3-2
1995
EN 61000-4-5
(2001)
EN 61000-3-3
1995
EN 61000-4-6
(2001)
EN 61000-4-2
(2001)
EN 61000-4-11
(2001)
CFR 47 Part 15 Subpart B: 2004
Code of Federal Regulations, Part 15, Subpart B, Radio Frequency Devices Unintentional Radiators
Class A
Low-Voltage Safety Compliance
This device complies with the EU directive 73/23/EEC (equivalent to IEC 1010-1, 1990 plus Amendment 1, 1991
and Amendment 2, 1995) by meeting the following standard: EN 61010-1: 2001 with Corrigendum No. 1 and 2.
FCC Compliance Statement
THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECT TO
THE FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFUL
INTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED,
INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION.
CAUTION Read and understand the various precautionary notes, signs, and symbols contained inside
this manual pertaining to the safe use and operation of this product before using the device.
Notice on Lifting and Handling of
Thermo Scientific Instruments
For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher Scientific
instrument requires a team effort to lift and/or move the instrument. This instrument is too heavy and/or bulky for one
person alone to handle safely.
Notice on the Proper Use of
Thermo Scientific Instruments
In compliance with international regulations: This instrument must be used in the manner specified by Thermo Fisher
Scientific to ensure protections provided by the instrument are not impaired. Deviations from specified instructions on
the proper use of the instrument include changes to the system and part replacement. Accordingly, order replacement
parts from Thermo Fisher Scientific or one of its authorized representatives.
Notice on the Susceptibility
to Electromagnetic Transmissions
Your instrument is designed to work in a controlled electromagnetic environment. Do not use radio frequency
transmitters, such as mobile phones, in close proximity to the instrument.
For manufacturing location, see the label on the instrument.
WEEE Compliance
This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE)
Directive 2002/96/EC. It is marked with the following symbol:
Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union
(EU) Member State, and these companies should dispose of or recycle this product. See www.thermoscientific.com/
rohsweee for further information on Thermo Fisher Scientific’s compliance with these Directives and the recyclers in
your country.
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Dieses Produkt muss die EU Waste Electrical & Electronic Equipment (WEEE) Richtlinie 2002/96/EC erfüllen.
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über die Einhaltung dieser Anweisungen durch Thermo Fisher Scientific, über die Verwerter, und weitere Hinweise,
die nützlich sind, um die Produkte zu identifizieren, die unter diese RoHS Anweisung fallen, finden sie unter
www.thermoscientific.com/rohsweee.
Conformité DEEE
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Electroniques (DEEE). Il est marqué par le symbole suivant:
Thermo Fisher Scientific s'est associé avec une ou plusieurs compagnies de recyclage dans chaque état membre de
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disponibles sur www.thermoscientific.com/rohsweee.
C
Contents
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi
Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xi
Safety and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xii
Safety Precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiii
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiv
Thermo Scientific
Chapter 1
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Why Use the MSQ Plus Mass Detector? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Is ESI or APCI Better for Analyzing My Samples? . . . . . . . . . . . . . . . . . . . . . . . . 3
Electrospray (ESI) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Atmospheric Pressure Chemical Ionization (APCI) . . . . . . . . . . . . . . . . . . . . . 5
How Do I Introduce My Samples into the Mass Detector? . . . . . . . . . . . . . . . . . 5
What Types of Buffers Can I Use?. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
How Do I Set Up the Mass Detector for Various LC Flow Rates?. . . . . . . . . . . . 6
How Do I Calibrate the MSQ Plus Mass Detector? . . . . . . . . . . . . . . . . . . . . . . 7
How Do I Optimize the Mass Detector Parameters for My Analytes? . . . . . . . . . 8
How Do I Create Instrument Control Methods?. . . . . . . . . . . . . . . . . . . . . . . . . 9
Chapter 2
Configuring Your LC/MS Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
Configuring the Mass Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Configuring the LC System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Chapter 3
Preparing Your LC/MS for Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15
Checking the Hardware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Checking the Status of the LC/MS Instrument . . . . . . . . . . . . . . . . . . . . . . . . . 16
Checking the Oil Level in the Oil Mist Filter and the Forepump . . . . . . . . . . . 18
Preparing the LC for Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
MSQ Plus Mass Detector Getting Started Guide
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Contents
viii
Chapter 4
Calibrating the MSQ Plus Mass Detector. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23
Preparing the MSQ Plus Mass Detector for Calibration . . . . . . . . . . . . . . . . . . 24
Verifying that the MSQ Plus Mass Detector is Set Up for ESI Mode . . . . . . 24
Setting Up the LC System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Checking the Solvent Levels in the Reference Inlet System . . . . . . . . . . . . . . 25
Setting the Gas Flows and Probe Heater Temperature. . . . . . . . . . . . . . . . . . 26
Baking Out the Probe (Optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Flushing Air from the Lines of the Reference Inlet System. . . . . . . . . . . . . . . 28
Viewing the Mass Spectrum of the Calibrant Solution (Optional). . . . . . . . . 30
Performing a Full-System Autotune . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Performing a Mass-Scale Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Performing a Detector Gain Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Chapter 5
Manual Tuning in ESI Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39
Setting Up the LC for Direct Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Selecting Your Tune Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Acquiring and Viewing a Raw Data File . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Chapter 6
Manual Tuning in the APCI Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49
Opening the Instrument Setup Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Selecting Your Tune Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Acquiring and Viewing a Raw Data File . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Chapter 7
Creating Instrument Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57
Welcome Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Method Editor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Method Options View. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Chromatogram View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
Per Method Parameters Table . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Full Scan Events Table . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
SIM Scan Events Table . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Menus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Creating an LC/MS Instrument Method. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Opening the Instrument Setup Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Specifying the Method Parameters for the MSQ Plus Mass Detector . . . . . . 65
Specifying the Method Parameters for the LC System . . . . . . . . . . . . . . . . . . 70
Saving the Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
Chapter 8
Creating and Running Sequences . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .75
Creating a Single Sample Sequence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Opening Sequence Setup Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Creating the Sequence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Saving the Sequence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
MSQ Plus Mass Detector Getting Started Guide
Thermo Scientific
Contents
Starting Data Acquisition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
Opening a Sequence File . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Selecting the Rows That You Want to Run . . . . . . . . . . . . . . . . . . . . . . . . . . 82
Selecting the Run Options and Starting the Run . . . . . . . . . . . . . . . . . . . . . . 82
Viewing the Data as It Is Acquired . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
Reviewing Real-Time Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
Adding Cells to the Display. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
Chapter 9
Using Qual Browser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .89
Cell States . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
Inactive Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
Active but Unpinned Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
Active and Pinned Cells. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
Cursor Actions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
Opening a Raw Data File in Qual Browser . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
Adding Cells to the Grid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
Viewing Reports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
Viewing the Mass Spectrum for a Specific Time Point . . . . . . . . . . . . . . . . . . . 98
Appendix A Sample Formulations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .101
Sensitivity Test Solutions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102
Preparing the Stock Solutions for the Sensitivity Tests. . . . . . . . . . . . . . . . . 102
Preparing the Working Solutions for the Sensitivity Tests . . . . . . . . . . . . . . 103
Preparing the MSQ Plus Mass Detector Calibration Solution . . . . . . . . . . . . . 105
Kit Contents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
Preparing the Calibration Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
Calibrant Solution. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106
Preparing a Concentrated Stock Solution of the Calibration Mixture . . . . . 107
Preparing the Working Calibration Solution . . . . . . . . . . . . . . . . . . . . . . . . 107
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .109
Thermo Scientific
MSQ Plus Mass Detector Getting Started Guide
ix
P
Preface
This MSQ Plus Mass Detector Getting Started Guide explains how to set up, calibrate, and tune
the Thermo Scientific™ MSQ Plus™ Mass Detector and how to acquire LC/MS data.
This manual supports the release of the Thermo MSQ™ 2.0 instrument software shipped with
the Thermo Xcalibur™ data system version 2.2 SP1 or later.
Contents
• Related Documentation
• Safety and Special Notices
• Safety Precautions
• Contacting Us
 To suggest changes to documentation or to Help
Complete a brief survey about this document by clicking the button below.
Thank you in advance for your help.
Related Documentation
In addition to this guide, Thermo Fisher Scientific provides the following documents as PDF
files for the MSQ Plus Mass Detector:
• MSQ Plus Mass Detector Hardware Manual
• MSQ Plus Mass Detector Getting Connected Guide
• MSQ Plus Mass Detector Preinstallation Guide
• MSQ Plus Mass Detector Calmix Kit Preparation Guide
• Dionex AXP/AXP-MS Metering Pump Operator’s Manual
Thermo Scientific
MSQ Plus Mass Detector Getting Started Guide
xi
Preface
Safety and Special Notices
• Dionex Chromelon/MSQ Plus Operator’s Guide
• Dionex MSQ Plus Facilities Preinstallation Requirements Guide
• Dionex MSQ Hardware Manual
• Dionex Installation and Commissioning Guide
• Dionex MSQ Preventive Maintenance
• Dionex MSQ10LA Nitrogen Generator for Mass Spectrometers
• Dionex MSQ18LA Nitrogen Generator for Mass Spectrometers
• Dionex N118LOA/N418LA Unpacking & Installation
• Dionex N*18KLA Nitrogen Generator User Manual
• Dionex MSQ Getting Started
• Safety and Regulatory Guide
You also receive a printed copy of the Safety and Regulatory Guide with your MSQ Plus
Mass Detector. This guide contains important safety information about Thermo
Scientific LC and MS systems. Make sure that all lab personnel have read and have access
to this document.
The software also provides Help.
Safety and Special Notices
Make sure you follow the precautionary statements presented in this guide. The safety and
other special notices appear in boxes.
Safety and special notices include the following:
CAUTION Highlights hazards to humans, property, or the environment. Each CAUTION
notice is accompanied by an appropriate CAUTION symbol.
IMPORTANT Highlights information necessary to prevent damage to software, loss of
data, or invalid test results; or might contain information that is critical for optimal
performance of the system.
Note Highlights information of general interest.
Tip Highlights helpful information that can make a task easier.
xii
MSQ Plus Mass Detector Getting Started Guide
Thermo Scientific
Preface
Safety Precautions
Safety Precautions
Observe the following safety precautions when you operate or perform service on the MSQ
Plus Mass Detector:
CAUTION Do not perform any servicing other than that contained in the MSQ Plus
Mass Detector Hardware Manual. To avoid personal injury or damage to the
instrument, do not perform any servicing other than that contained in the MSQ Plus Mass
Detector Hardware Manual or related manuals unless you are qualified to do so.
CAUTION Shut down the mass detector and disconnect it from line power before you
service it. High voltages capable of causing personal injury are used in the instrument.
Some maintenance procedures require that the mass detector be shut down and
disconnected from line power before service is performed. Do not operate the mass
detector with the top or side covers off. Do not remove protective covers from PCBs.
CAUTION Do not interfere with the safety interlock. Interfering with the safety
interlock will expose you to potentially lethal electrical hazards.
CAUTION Respect heated zones. Treat heated zones with respect. The ion transfer
capillary and the APCI vaporizer might be very hot and might cause severe burns if
touched. Allow heated components to cool before you service them.
CAUTION Place the mass detector in Standby (or Off ) mode before you open the
atmospheric pressure ionization (API) source. The presence of atmospheric oxygen in
the API source when the mass detector is on could be unsafe. The mass detector
automatically goes into standby mode when you open the API source; however, take this
added precaution for safety reasons.
CAUTION Take care when handling the corona pin. The corona pin is sharp and can
cause personal injury. Take care when removing or installing the corona pin.
CAUTION Make sure you have sufficient nitrogen for your API source. Before you
begin normal operation each day, make sure that you have sufficient nitrogen for your API
source. The presence of atmospheric oxygen in the API source when the mass detector is
on could be unsafe.
Thermo Scientific
MSQ Plus Mass Detector Getting Started Guide
xiii
Preface
Contacting Us
CAUTION Contain waste streams. Because the API source can accommodate high
solvent flow rates, you must make provisions to collect the waste solvent.
CAUTION Provide adequate fume exhaust systems for the API source solvent waste
container and the forepump. Your laboratory must be equipped with at least two fume
exhaust systems: one to vent the waste container connected to the exhaust port (API
solvent drain) on the back of the mass detector and the other to vent the forepump
exhaust. As described in the MSQ Plus Mass Detector Getting Connected Guide, route the
(blue) forepump exhaust hose to a dedicated fume exhaust system. Because the exhaust
hose acts as a trap for exhaust fumes that would otherwise recondense in the forepump oil,
the hose should travel at floor level for a minimum of two meters (78.5 in.) before it
reaches the external exhaust system. Route tubing from the waste container connected to
the exhaust port on the back of the mass detector to a second dedicated fume exhaust
system. Consult local regulations for the proper method of exhausting the fumes from
your system.
Do not vent the PVC drain tube (or any vent tubing connected to the waste container) to
the same fume exhaust system that is connected to the forepump. The forepump exhaust
contains pump oil, which can seriously contaminate the analyzer optics of the mass
spectrometer.
Contacting Us
There are several ways to contact Thermo Fisher Scientific for the information you need.
 To contact Technical Support
Phone
800-532-4752
Fax
561-688-8736
E-mail
[email protected]
Knowledge base
www.thermokb.com
Find software updates and utilities to download at mssupport.thermo.com.
 To contact Customer Service for ordering information
xiv
Phone
800-532-4752
Fax
561-688-8731
E-mail
[email protected]
Web site
www.thermo.com/ms
MSQ Plus Mass Detector Getting Started Guide
Thermo Scientific
Preface
Contacting Us
 To get local contact information for sales or service
Go to www.thermoscientific.com/wps/portal/ts/contactus.
 To copy manuals from the Internet
Go to mssupport.thermo.com, agree to the Terms and Conditions, and then click
Customer Manuals in the left margin of the window.
 To suggest changes to documentation or to Help
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• Send an e-mail message to the Technical Publications Editor at
[email protected]
Thermo Scientific
MSQ Plus Mass Detector Getting Started Guide
xv
1
Introduction
This chapter explains the basic functionality of the MSQ Plus Mass Detector and the
preparations for using the tool.
Contents
• Why Use the MSQ Plus Mass Detector?
• Is ESI or APCI Better for Analyzing My Samples?
• How Do I Introduce My Samples into the Mass Detector?
• What Types of Buffers Can I Use?
• How Do I Set Up the Mass Detector for Various LC Flow Rates?
• How Do I Calibrate the MSQ Plus Mass Detector?
• How Do I Optimize the Mass Detector Parameters for My Analytes?
• How Do I Create Instrument Control Methods?
The MSQ Plus Mass Detector is a high-performance single-quadrupole mass spectrometer. It
includes a reference inlet system, an atmospheric pressure ionization (API) source, and the
Xcalibur data system. The reference inlet system is used for autotuning and mass-scale
calibration.
In liquid chromatography mass spectrometry (LC/MS) analysis, an autosampler injects a
sample onto an LC column. The sample separates into its various components. The
components elute from the LC column and pass into the mass detector, where they are
analyzed. The Xcalibur data system then stores and processes the data from the mass detector.
Why Use the MSQ Plus Mass Detector?
The MSQ Plus Mass Detector can increase the specificity and detection limits for applications
that use any of the classic detectors, such as UV, fluorescence, light-scattering, or refractive
index. Applications using classic detectors rely primarily on chromatographic retention times
to identify analytes. In contrast, a mass detector allows mass identification for the components
of chromatographic peaks.
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Introduction
Why Use the MSQ Plus Mass Detector?
A mass detector consists of an ion source, a mass analyzer, a vacuum system, and an ion
detector. The ion source performs four functions: it separates the analytes from the solvent
molecules, it ionizes the analytes, it drives the ionized solutes from the liquid phase to the gas
phase, and it provides efficient transfer of the ions to the mass analyzer. The vacuum system
provides the driving force for drawing ions into the mass analyzer and the environment in
which the ions can reach the detector without colliding with other gaseous molecules. The
mass analyzer produces an electric field or a magnetic field or both that allow only ions within
a specified mass-to-charge range to reach the ion detector. The ion detector detects the
presence of ions as they exit the mass analyzer.
The MSQ Plus Mass Detector performs two types of complementary atmospheric pressure
ionization: electrospray (ESI) and atmospheric pressure chemical ionization (APCI). It uses a
transfer lens to transmit ions to the mass analyzer. The mass analyzer consists of a single round
quadrupole with an upper mass range limit of 2000 Da at unit resolution. The vacuum
system consists of an external forepump and an internal turbomolecular pump. The external
forepump operates from atmospheric pressure down to 10–3 torr and handles high gas loads
of approximately 30 m3/h at 1 torr. The internal turbomolecular pump provides a high
vacuum of 10–6 torr. The ion detector is a conversion dynode, which allows a time interval of
200 milliseconds for switching between positive and negative polarity modes.
The MSQ Plus Mass Detector measures only 30 cm (12 in.) wide and integrates seamlessly
with the Accela™ LC system. The following new innovations for this mass detector make it
ideal for the chromatographer:
• ESI and APCI FastLoc™ probes, which allow you to switch between the two ionization
modes in less than a minute
• Wipe-clean, M-Path™ ion source with a patented cone wash system for ultra-rugged
performance with non-volatile phosphate buffers
• Automated full-system autotune
• Interactive method setup with 10 built-in editable templates
The M-Path source and the cone wash system allow you to use mobile phases that would
ordinarily be prohibited because of their tendency to clog the entrance cone of the ion source.
The positioning of the probe insert directs non-volatile components, such as those from
mobile-phase buffers and ion-pairing agents or sample matrices, away from the orifice of the
entrance cone. A low flow of solvent (100 to 250 L/min) from the cone wash system
spraying the edge of the entrance orifice helps to prevent a build-up of non-volatiles during an
LC/MS run.
The tuning and calibration process that is required for all quadrupole mass analyzers to
optimize their mass range, mass accuracy, and mass resolution is completely automated with
wizards. Figure 1 shows the user interface for these wizards. The service engineer tunes and
calibrates the detector at installation. Perform an automated mass scale calibration whenever
you notice a drift in the accuracy of the reported masses of your known standards.
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Is ESI or APCI Better for Analyzing My Samples?
Figure 1.
Instrument Tuning and Calibration wizard
To create an acquisition method for an LC/MS system with an MSQ Plus Mass Detector, use
the Tune window to optimize the parameters for your analyte. After determining the optimal
mass detector settings for your analyte, open a template from the Instrument Setup window,
import the Tune file that contains the optimal settings for your analyte, and modify a few
parameters, if necessary.
Is ESI or APCI Better for Analyzing My Samples?
You can operate the MSQ Plus Mass Detector in either of two atmospheric pressure
ionization modes: electrospray ionization (ESI) or atmospheric pressure chemical ionization
(APCI).
Typically, polar compounds such as amines, peptides, and proteins are best analyzed by ESI,
and non-polar compounds such as steroids are best analyzed by APCI.
Sample ions can carry a single charge or multiple charges. The number of charges carried by
the sample ions depends on the structure of the analyte of interest, the mobile phase, and the
ionization mode.
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Is ESI or APCI Better for Analyzing My Samples?
Electrospray (ESI)
ESI mode generally produces mass spectra consisting of singly charged ions, but it depends on
the structure of the analyte and the solvent. When multiply charged ions are produced, the
Xcalibur data system can mathematically transform the resulting mass spectrum to express the
molecular weight of the sample.
ESI mode transfers ions in solution into the gas phase. Because the ESI process does not use
heat to nebulize the analyte ions, you can use it to analyze heat-labile compounds or
high-molecular-weight compounds.
You can use ESI mode to analyze any polar compound that makes a preformed ion in
solution. Preformed ions can include adduct ions. For example, polyethylene glycols can be
analyzed from a solution containing ammonium acetate because of adduct formation between
the NH4+ ions in the solution and oxygen atoms in the polymer. With ESI, the MSQ Plus
Mass Detector can analyze compounds with molecular weights that are greater than
100000 Da because of multiple charging. ESI is especially useful for the mass analysis of polar
compounds, which include biological polymers (for example, proteins, peptides,
glycoproteins, and nucleotides), pharmaceuticals and their metabolites, and industrial
polymers.
You can use ESI mode in either the positive- or negative-ion polarity mode. Matching the
charge on the ESI probe to the charge on the preformed ions in solution increases the
sensitivity of the analysis. Therefore, select the ion polarity mode according to the polarity of
the preformed ions in solution. Acidic molecules form negative ions in high pH solution, and
basic molecules form positive ions in low pH solution.
You can vary the flow rate in ESI mode from the LC into the mass detector from 10 L/min
to 2000 L/min. (In ESI, the buffer type and the buffer strength both have a noticeable effect
on sensitivity. Therefore, it is important to choose these variables correctly.) In the case of
higher-molecular-weight proteins or peptides, the resulting mass spectrum typically consists
of a series of peaks corresponding to a distribution of multiply charged analyte ions.
The droplet size, surface charge, liquid surface tension, solvent volatility, and ion solvation
strength all affect the ESI process. Large droplets with high surface tension, low volatility,
strong ion solvation, low surface charge, and high conductivity prevent good electrospray.
Mixed organic-aqueous solvent systems that include organic solvents, such as methanol,
acetonitrile, and isopropyl alcohol, are superior to water alone for ESI. Volatile acids and bases
are good, but Thermo Fisher Scientific does not recommend salts above 10 mM. Strong
mineral acids and bases are extremely detrimental to the instrument.
Observe the following recommendations for generating stable electrospray:
• Refrain from using non-volatile salts and buffers in the solvent system. For example, avoid
the use of salts containing sodium or potassium and avoid the use of phosphates.
If necessary, use ammonium salts instead.
• Use organic-aqueous solvent systems.
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How Do I Introduce My Samples into the Mass Detector?
• Use volatile acids and bases.
• If possible, optimize the pH of the solvent system for your analyte of interest. For
example, if your analyte of interest contains a primary or secondary amine, use an acidic
mobile phase (pH 2 to 5) for your chromatographic separation. The acidic pH tends to
keep positive ions in solution.
Atmospheric Pressure Chemical Ionization (APCI)
Like ESI, APCI is a soft ionization technique, as opposed to an electron ionization technique.
APCI provides molecular weight information for compounds of low polarity that are
somewhat volatile. APCI is typically used to analyze small molecules with molecular weights
up to about 1000 Da.
APCI is a gas-phase ionization technique. Therefore, the gas phase acidities and basicities of
the analyte and solvent vapor play an important role in the APCI process.
In APCI mode, you can vary the flow rate from the LC into the mass detector from 0.2 to
2 mL/min.
You can use APCI in positive- or negative-ion polarity mode. Molecules with basic sites
produce a strong ion current in positive ion mode. Molecules with acidic sites, such as
carboxylic acids and acid alcohols, produce a strong ion current in negative-ion mode.
Although, in general, fewer negative ions are produced than positive ions, the negative ion
polarity mode can be more specific, because the negative ion polarity mode typically generates
less chemical noise than does the positive mode. Therefore, the signal-to-noise ratio might be
better in the negative ion mode than in the positive ion mode.
How Do I Introduce My Samples into the Mass Detector?
Use an LC system containing an LC pump and an autosampler to introduce samples into the
MSQ Plus Mass Detector. The MSQ Plus Mass Detector has a reference inlet system for the
introduction of the tuning and mass scale calibration solution.
The Xcalibur data system controls HPLC systems manufactured by Thermo Fisher Scientific
and other manufacturers. The Accela LC system manufactured by Thermo Fisher Scientific
integrates seamlessly with the MSQ Plus Mass Detector.
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What Types of Buffers Can I Use?
What Types of Buffers Can I Use?
Use volatile buffers, when possible, to obtain the highest performance for your assays. Volatile
buffers include the following:
• Acetic acid
• Ammonium acetate
• Ammonium formate
• Ammonium hydroxide
• Triethylamine (TEA)
• Trifluoroacetic acid (TFA)
Some LC applications use non-volatile buffers, such as phosphate or borate buffers. However,
the use of non-volatile buffers can lead to a buildup of salt in the ion source and can cause a
loss of sensitivity.
For LC applications that require non-volatile buffers, Thermo Fisher Scientific recommends
installing the optional cone wash pump.
Note You might need to increase the frequency of ion source maintenance when using
non-volatile buffers.
How Do I Set Up the Mass Detector for Various LC Flow Rates?
The ESI probe can generate ions from liquid flows of 10 μL/min to 2.0 mL/min. This flow
rate range lets you use a variety of separation techniques: capillary LC, microbore LC, and
analytical LC. The APCI probe can generate ions from liquid flows of 0.2 to 2.0 mL/min.
Within this flow rate range, you can use the microbore LC, analytical LC, and
semi-preparative LC separation techniques.
CAUTION Do not heat the probe without the nitrogen gas flowing. To prepare the mass
detector for operation, turn on the nitrogen gas, turn on the probe heater, and then turn
on the LC flow. After you finish an analysis, turn off the LC flow, turn off the heater, and
then turn off the nitrogen gas.
As you change the solvent flow rate entering the mass detector, adjust the mass detector
parameters:
• For ESI mode, adjust the probe temperature and the needle voltage.
• For APCI mode, adjust the probe temperature and the corona current.
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How Do I Calibrate the MSQ Plus Mass Detector?
In general, increase the probe temperature as you increase the flow rate from the LC pump.
Table 1 and Table 2 list the suggested starting probe temperatures for optimizing an analysis
in ESI mode or APCI mode, respectively. You might find that the optimal probe temperature
for your analysis differs from these values by as much as 50 °C.
Table 1. Starting probe temperature values for ESI mode
Flow rate
Temperature
200 μL/min
350 °C
500 μL/min
450 °C
1000 μL/min
575 °C
2000 μL/min
650 °C
Table 2. Starting probe temperature values for APCI mode
Flow rate
Temperature
200 μL/min
450 °C
500 μL/min
550 °C
1000 μL/min
650 °C
2000 μL/min
650 °C
How Do I Calibrate the MSQ Plus Mass Detector?
The MSQ Plus Mass Detector includes a comprehensive autotune system that offers two
levels of instrument calibration: full-system autotune and mass-scale calibration.
When installing the system, the Thermo Fisher Scientific service engineer performs a
full-system autotune, which includes a mass-scale calibration. After receiving a full-system
autotune, the mass detector is extremely stable in the region of 0.01 Da/°C. In a normal
air-conditioned laboratory, the mass detector rarely, if ever, needs a second full-system
autotune.
A deep tune is an automated operation that adjusts the operating frequency of the RF
generator to match the natural resonant frequency of the quadrupole analyzer. It ensures that
the mass detector operates to the maximum value of its mass range.
Resolution set-up is an automated operation that electronically adjusts the resolution of the
peaks produced by the calibrant at both high and low mass (1971.6 m/z and 172.8 m/z,
respectively). It is adjusted to give unit resolution by setting the peak width to 0.7 Da at the
half height of the peak. It also performs a linearity adjustment to the same criterion at mid
mass (1072.24 m/z).
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How Do I Optimize the Mass Detector Parameters for My Analytes?
Mass-scale calibration is an automated procedure that compares the masses of the
factory-supplied calibrant solution to a reference file of the same compound. The reference
file contains the correct mass for each peak and the values of the acquired data file are
corrected to match those in the reference file. These correction factors are then applied to all
subsequent acquisitions.
Perform a full-system autotune when you do the following:
• Move the mass detector to a different environment.
• Perform maintenance on the source block.
• Install or update the Xcalibur software.
Perform a mass-scale calibration if you notice a small drift in mass accuracy.
A full-system autotune does the following:
1. Resets all tune parameters to their default values.
2. Performs a deep tune.
3. Performs a resolution set-up.
4. Performs a mass-scale calibration.
The default tune parameters for your MSQ Plus Mass Detector are set at the factory.
For information on tuning and calibrating the MSQ Plus Mass Detector, see “Calibrating the
MSQ Plus Mass Detector” on page 23.
How Do I Optimize the Mass Detector Parameters for My Analytes?
Optimize the run acquisition parameters for your MSQ Plus Mass Detector from the Tune
window. For ESI mode, optimize the probe temperature, needle voltage, and cone voltage. For
APCI mode, optimize the probe temperature, corona current, and cone voltage.
The optimization procedure requires the use of an LC system containing both an LC pump
and an autosampler. The autosampler introduces your sample into the solvent flow produced
by the LC pump. An LC column is not required for this procedure. However, because LC
pumps require a minimum backpressure of 3 bar (40 psi) for optimal performance, you might
need to connect a backpressure regulator between the LC outlet and mass detector inlet.
After you optimize these settings in the Tune window, save them in a tune file. When you
create an instrument method, import the tune file into the Method Editor.
The Xcalibur data system imports these parameters with the tune file:
• Needle voltage or corona current
• RF lens bias
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How Do I Create Instrument Control Methods?
• Ion energy
• Low-mass resolution
• High-mass resolution
You cannot modify the RF lens bias, ion energy, low-mass resolution, and high-mass
resolution parameters. The correct values are embedded when you use any of the following
files as a template for optimization: the esipos.tune, esineg.tune, apcipos.tune, or apcineg.tune
file.
After you import the tune file into the Method Editor, you must manually enter the optimal
probe temperature and cone voltages for your application.
For information on creating tune files for ESI experiments, see “Manual Tuning in ESI Mode”
on page 39. For information on creating tune files for APCI experiments, see “Manual Tuning
in the APCI Mode” on page 49. For information on creating instrument methods, see
“Creating Instrument Methods” on page 57.
How Do I Create Instrument Control Methods?
Create instrument methods, which control all the modules of your LC/MS instrument, from
the Xcalibur Instrument Setup window. You manually enter the parameters for the modules of
your instrument, with the exception of either the needle voltage or the corona current value
for the MSQ Plus Mass Detector.
For ESI mode, import a tune (.tune) file that contains the appropriate needle voltage for your
application. For APCI mode, import a tune (.tune) file that contains the appropriate corona
current for your application.
The Use Current Tune file check box is selected by default, as shown in Figure 2. This means
that the mass detector uses the needle voltage or the corona current value in the current tune
file. To use the needle voltage value or the corona current value from a stored tune file, you
must deselect this check box, click the Browse button to the right of the Use Selected Tune
File box, and select an appropriate tune file.
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How Do I Create Instrument Control Methods?
Figure 2.
MSQ Plus Method Editor dialog box
Use current tune file
check box
Browse button
For information on making automated injections, consult the following chapters in this
manual:
• For information on creating tune files for ESI experiments, see “Manual Tuning in ESI
Mode” on page 39.
• For information on creating tune files for APCI experiments, see “Manual Tuning in the
APCI Mode” on page 49.
• For information on creating instrument methods, see “Creating Instrument Methods” on
page 57.
• For information on creating a sequence spreadsheet to automate the injection of a set of
samples, see “Creating and Running Sequences” on page 75.
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Configuring Your LC/MS Instrument
This chapter describes how to configure your LC/MS instrument.
The typical inlet for the MSQ Plus Mass Detector is a liquid chromatograph. The Xcalibur
2.2 SP1 data system supports liquid chromatography systems manufactured by Thermo
Fisher Scientific and other manufacturers.
During installation, a Thermo Fisher Scientific service engineer will connect your MSQ Plus
Mass Detector and a supported liquid chromatograph. In addition, the service engineer will
add these supported devices to your Xcalibur instrument configuration. To modify your
LC/MS instrument, refer to the MSQ Plus Mass Detector Getting Connected Guide for
instructions on connecting the modules of a liquid chromatograph to your MSQ Plus Mass
Detector.
Various configuration options are available for each of the supported liquid chromatographs.
In addition, you can specify the serial number and a location code, company asset ID, or
custom label for the MSQ Plus Mass Detector. Before you operate your system, verify that
your instrument contains the appropriate configuration settings.
Contents
• Configuring the Mass Detector
• Configuring the LC System
Configuring the Mass Detector
You can track the serial number of the MSQ Plus Mass Detector used in experiments by
entering its serial number in the MSQ Plus Configuration dialog box. The serial number
appears in the autotune report.
 To add the MSQ Plus Mass Detector to your instrument configuration
1. From the Windows™ taskbar in Xcalibur 2.2 SP1or later, choose Start > Programs
>Thermo Foundation x.x > Instrument Configuration.
The Thermo Foundation Instrument Configuration window appears, as shown in
Figure 3.
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Configuring Your LC/MS Instrument
Configuring the Mass Detector
Figure 3.
Thermo Foundation Instrument Configuration window
2. If the MSQ Plus Mass Detector is not shown in the Configured Devices list, scroll down
through the devices shown in the Available Device list. Double-click the MSQ Plus
button to copy it to the Configured Devices list.
3. Double-click the MSQ Plus icon in the Configured Devices pane to open the MSQ Plus
Configuration dialog box, shown in Figure 4.
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Figure 4.
Configuring Your LC/MS Instrument
Configuring the LC System
MSQ Plus Configuration dialog box
4. (Optional) To enter the serial number of the mass detector, click in the Serial number
box, and then type the serial number.
The serial number is located on the back of the MSQ Plus Mass Detector.
5. (Optional) To enter user-defined information, select or type a value of up to 20 characters
in the Label box.
The available selections are Company Asset ID and Location code.
6. Type a value of up to 20 characters for the location code, company asset ID, or
user-defined label in the Value box.
7. Click OK to close the MSQ Plus Configuration dialog box.
8. Complete the configuration of your LC/MS system, and then click Done to exit the
Instrument Configuration application.
Configuring the LC System
If your LC system is controlled from the Xcalibur data system, you must add it or all of its
devices to your instrument configuration.
 To add your LC system to the instrument configuration for your data system
1. If a particular device is not listed in the Configured Devices pane in the Instrument
Configuration dialog box, double-click its icon in the Available Devices pane to add it to
the Configured Devices pane.
2. Double-click each device in the Configured Device pane and check its configuration
settings.
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Configuring Your LC/MS Instrument
Configuring the LC System
Information on the configuration settings for each device is available in the Help. For
additional information about your Accela LC system, refer to the Accela UHPLC System
User Guide for LC Devices.
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Preparing Your LC/MS for Operation
This chapter describes how to prepare your LC/MS instrument for operation. Check the
system before restarting its operation by performing the procedures in this chapter.
Contents
• Checking the Hardware
• Checking the Status of the LC/MS Instrument
• Checking the Oil Level in the Oil Mist Filter and the Forepump
• Preparing the LC for Operation
Checking the Hardware
First, check the hardware of your mass detector and your LC system.
 To check the hardware of the mass detector and the LC system
1. Verify that the power, communication, and nitrogen supply lines are correctly connected.
For instructions on connecting the MSQ Plus Mass Detector, refer to the MSQ Plus Mass
Detector Getting Connected Guide.
2. Check that the LC system is properly connected.
Instructions for connecting a Thermo Scientific LC are included in the manuals stored on
the LC devices CD that is part of the media kit. For detailed instructions on connecting
an Accela LC system, refer to the Accela Getting Connected Guide.
3. Verify that the nitrogen gas supply for the mass detector is adequate.
The MSQ Plus Mass Detector uses a maximum of 720 L/h in ESI mode.
4. Verify that the nitrogen regulator is set to 5.2 bar (75 psi) for ESI mode or 3.1 bar
(45 psi) for APCI mode.
The nitrogen regulator, shown in Figure 5, is located below the source compartment of
the mass detector.
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Preparing Your LC/MS for Operation
Checking the Status of the LC/MS Instrument
Figure 5.
Nitrogen regulator
Pressure
gauge
Control
dial
5. Verify that the hard drive on your data system computer contains sufficient storage space.
Note Anti-virus software uses a large portion of your computer’s memory as it scans
the hard drive for viruses. Ensure that the anti-virus software is not set to perform a
scan while you are acquiring data.
The Xcalibur data system does not include anti-virus software.
Checking the Status of the LC/MS Instrument
If the MSQ Plus Mass Detector has not been in use recently, it might be in one of four
possible states:
• The system might be under vacuum with the nitrogen gas flowing.
• The system might be in Off mode, which means that the nitrogen flow is reduced to a
bleed through the API probe, the electron multiplier and conversion dynode are turned
off, the power to the ion optics is turned off, and the power to the probe heater is turned
off, but the system is still under vacuum.
• The system might be vented, which means it is no longer under vacuum.
• The system might not be connected.
During typical usage, the MSQ Plus Mass Detector is either left on, with the nitrogen
flowing, or off, with both the nitrogen flow and probe heater turned off. The forepump is
always left powered on, and the source is always left under vacuum. If the system has been
moved or has been left unused for several months, it might be vented or powered off. For
instructions on pumping down a vented system, see the MSQ Plus Mass Detector Hardware
Manual.
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Checking the Status of the LC/MS Instrument
Note Do not vent the system unless you plan to move the MSQ Plus Mass Detector,
perform maintenance on an internal component of the system, or leave the system unused
for a period of several months.
For information about the status of the MSQ Plus Mass Detector, use the Server icon, shown
in Figure 6, and the Information view of the Xcalibur data system, shown in Figure 7. The
server software handles the communication between the MSQ Plus Mass Detector and the
Xcalibur data system. The Xcalibur data system controls the modules of your LC/MS system.
Figure 6.
Server icon
Server icon
Figure 7.
Xcalibur Roadmap view
Roadmap
view
Information
view
The Server icon shown in Figure 6 mimics a tristate LED display (red, green, yellow). Its
states have the following meanings:
• Steady red when the power is on and the instrument is vented or an error has occurred
within the system
• Flashing yellow when the instrument is pumping down
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Checking the Oil Level in the Oil Mist Filter and the Forepump
• Steady yellow when the instrument is pumped down but not in Operate mode
• Steady green when the instrument is pumped down and in Operate mode
 To check the status of your LC/MS instrument
1. Launch the Xcalibur data system by doing one of the following:
• From the Windows desktop, double-click the Xcalibur icon,
.
–or–
• For the Xcalibur 2.2 SP1 data system or later, choose Start > Programs > Thermo
Xcalibur > Xcalibur.
The data system opens to the Thermo Xcalibur Roadmap view, shown in Figure 7 on
page 17. If your instrument configuration contains an MSQ Plus Mass Detector, the
Server icon appears in the system tray of the Windows taskbar next to the time display.
If the Server icon does not appear when you start the data system, check the configuration
of your instrument and the USB cable connection to the MSQ Plus Mass Detector. You
do not need to add specific configuration information for the MSQ Plus Mass Detector,
but you do have to add the mass detector to your instrument configuration. See
“Configuring Your LC/MS Instrument” on page 11 and the Help for information on
configuring your instrument.
2. If the Information view is not displayed, click the Info View icon,
display it.
, in the toolbar to
Figure 7 shows the Status page of the Information view on the Roadmap view for an
LC/MS instrument that includes an Accela autosampler, an Accela pump, and an MSQ
Plus Mass Detector.
3. Click the listing of each configured device to display its status in the lower portion of the
Status page.
Checking the Oil Level in the Oil Mist Filter and the Forepump
The forepump is external to the MSQ Plus Mass Detector and requires routine maintenance
to keep it running at its optimum performance level.
Note You can find more information on operating and maintaining the forepump in the
manual that accompanies it.
As oil is vaporized by the forepump during normal operation, the oil mist filter fills with
condensed oil. In addition, the oil in the forepump becomes contaminated with volatile
solvents, which can cause corrosion and reduce the lifetime of the forepump. Once the oil
level in the oil mist filter rises above the maximum oil level mark, the oil mist filter becomes
ineffective in trapping exhaust fumes. See Figure 8.
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Checking the Oil Level in the Oil Mist Filter and the Forepump
Figure 8.
Edwards™ forepump and attached oil mist filter showing the maximum oil level for efficient performance of the
filter
To
exhaust vent
MSQ Plus Mass Detector
MAINS ON/OFF
PUMP OUT
GAS IN
6 BARS MAX
MAINS IN
EDWARDS
Oil Mist Filter EMF 20
Gas ballast knob
Maximum
oil level mark
Oil return tubing
EDWARDS
220 V ac
30
Check the level and color of the oil in the forepump (also referred to as a backing pump, a
roughing pump, or a rotary-vane pump) at least once a month. Check the oil level in the oil
mist filter that is attached to the Edwards™ forepump on a daily basis.
To check the oil level in the forepump, look through the oil sight glass at one end of the
forepump. The oil level should be between the upper and lower marks positioned next to the
window. See Figure 9. The oil color should be a clear light yellow.
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Preparing Your LC/MS for Operation
Checking the Oil Level in the Oil Mist Filter and the Forepump
Figure 9.
End of Edwards forepump showing oil-level window
Oil mist filter
Edwards forepump
EDWARDS
E32M30
Oil level window
Perform maintenance as necessary:
• If the oil level is near or below the lower mark, add more oil, as described in the manual
that comes with the Edwards forepump.
• If the oil has turned red in color or if the forepump has been in operation for more than
3000 hours since the oil was replaced, replace the oil, as described in the manual that
comes with the Edwards forepump.
 To drain the oil from the oil mist filter and to purge the oil of volatile contaminants
1. If the LC pump is pumping solvent, turn the pump flow off.
2. Put the MSQ Plus Mass Detector in Off mode by doing one of the following:
• From the Status page of the Information view in the Xcalibur data system, right-click
the MSQ Plus listing to display a shortcut menu, and choose Turn Device Off from
the menu.
–or–
• Open the Per Method Parameters table in the Tune window. Take the system out of
Operate mode by clicking the Operate On/Off toggle button. Then turn off the
nitrogen gas by clicking the Nitrogen Gas On/Off toggle button.
3. Open the gas ballast valve on the Edwards forepump by turning it six rotations
counterclockwise.
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Preparing the LC for Operation
4. Operate the Edwards forepump for approximately 15 minutes with the gas ballast valve
open. This allows enough time to return the oil to the forepump and to remove volatile
solvents such as water from the oil.
5. After you have ballasted the forepump to remove volatile solvents, close the gas ballast
valve by turning the gas ballast knob clockwise until you feel resistance.
Note Operating the forepump with the gas-ballast valve open increases the rate of oil
loss from the pump. During normal operations, run the forepump with the gas ballast
valve closed.
Preparing the LC for Operation
Before you tune your MSQ Plus Mass Detector or begin a sequence run, check the status of
your LC system. Verify that the solvent bottles contain the appropriate solvents and that the
solvent lines are free of air. If the modules of your liquid chromatograph are controlled from
the Xcalibur data system, verify that they are communicating with the data system and are
ready for operation.
If you have an Accela LC system, ensure that the wash bottle contains solvent and that the
syringe of the Accela autosampler and the four solvent reservoir lines are free of air. If
necessary, use the Flush command to remove air from the syringe and the tubing that
connects the syringe to the wash bottle. If you are using an Accela pump, use a Purge
command to remove air from the solvent reservoir lines or to draw fresh solvent through the
lines. For more information on the Accela LC system, refer to the Accela UHPLC System User
Guide for LC Devices.
Note If you are using a viscous wash solvent, such as a methanol/water mix, reduce the
flush speed for the Accela autosampler from its default of 400 to 100 μL/s.
Thermo Scientific
MSQ Plus Mass Detector Getting Started Guide
21
4
Calibrating the MSQ Plus Mass Detector
The MSQ Plus Mass Detector software includes an automated wizard that provides three
levels of calibration:
• Full-system autotune: This procedure is performed by a Thermo Fisher Scientific service
engineer during the initial installation of the MSQ Plus Mass Detector. You do not need
to repeat this procedure unless you perform maintenance on the inner components of the
source block or reinstall or upgrade the Xcalibur software.
• Mass-scale calibration: This procedure is a subset of the full-system autotune procedure.
You should perform a mass-scale calibration if you notice a drift in the mass accuracy of
your analyses or every 3 to 6 months as part of your routine maintenance schedule for the
MSQ Plus Mass Detector.
• Detector gain calibration: This procedure resets the gain of the electron multiplier as it
ages with use.
Note To perform any of these calibration procedures, you must connect an LC pump that
is capable of delivering solvent at a flow rate of 200 μL/min to your MSQ Plus Mass
Detector. See the MSQ Plus Mass Detector Getting Connected Guide for instructions on
connecting an LC system to your MSQ Plus Mass Detector.
Before you perform a full-system autotune or a mass-scale calibration, prepare your MSQ Plus
Mass Detector as described in “Preparing Your LC/MS for Operation” on page 15.
Contents
• Preparing the MSQ Plus Mass Detector for Calibration
• Performing a Full-System Autotune
• Performing a Mass-Scale Calibration
• Performing a Detector Gain Calibration
Thermo Scientific
MSQ Plus Mass Detector Getting Started Guide
23
4 Calibrating the MSQ Plus Mass Detector
Preparing the MSQ Plus Mass Detector for Calibration
Preparing the MSQ Plus Mass Detector for Calibration
To perform a full-system autotune, mass-scale calibration, or detector gain calibration, you
must set up the mass detector for ESI mode and ensure that the reference inlet reservoir
contains a sufficient volume of factory-supplied calibrant.
To prepare the MSQ Plus Mass Detector for calibration, follow these procedures:
1. Verifying that the MSQ Plus Mass Detector is Set Up for ESI Mode
2. Setting Up the LC System
3. Checking the Solvent Levels in the Reference Inlet System
4. Setting the Gas Flows and Probe Heater Temperature
5. Baking Out the Probe (Optional)
6. Flushing Air from the Lines of the Reference Inlet System
7. Viewing the Mass Spectrum of the Calibrant Solution (Optional)
Verifying that the MSQ Plus Mass Detector is Set Up for ESI Mode
To perform a full-system autotune or a mass-scale calibration, the MSQ Plus Mass Detector
must be set up in ESI mode. Figure 10 shows an MSQ Plus Mass Detector set up for
operation in ESI mode.
Figure 10. MSQ Plus Mass Detector set up for operation in the ESI mode
 To verify that the mass detector is set up for ESI mode
1. Open the front door of the mass detector by depressing the latch on the left side of the
mass detector.
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MSQ Plus Mass Detector Getting Started Guide
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4 Calibrating the MSQ Plus Mass Detector
Preparing the MSQ Plus Mass Detector for Calibration
Note When the front door is open, do not attempt to insert a screwdriver or other
device into the safety interlock switch, because a severe shock can result.
2. Ensure that the ESI probe is connected and that the corona pin knob is in the vertical
position.
For instructions on switching your mass detector to ESI mode, see the MSQ Plus Mass
Detector Hardware Manual.
Setting Up the LC System
To perform a full-system autotune or a mass-scale calibration, connect an LC pump to your
MSQ Plus Mass Detector. The LC pump must be set to deliver 50:50 acetonitrile/water at a
flow rate of 200 μL/min. If your LC system is set up for chromatography, remove the LC
column and replace it with a backpressure regulator.
 To set up the LC pump
1. For Xcalibur 2.2 SP1 or later, choose Start > Programs > Thermo Xcalibur > Xcalibur.
On the Roadmap view in the Xcalibur data system, click the Instrument Setup icon.
The Instrument Setup window appears with icons for all of the currently configured
devices for your LC/MS instrument displayed down the left side of the window.
Instrument Setup pages and dialog boxes for the selected device are displayed on the right
side of the view. You can access additional dialog boxes for the device by clicking the
device listing in the menu bar.
2. Open the Direct Control dialog box for your LC pump.
3. Leave the Direct Control dialog box open, and adjust the size of the Instrument Setup
window to half the size of your view screen.
Checking the Solvent Levels in the Reference Inlet System
The reference inlet system includes a reference inlet reservoir bottle and a waste bottle. To
perform a full-system autotune or a mass-scale calibration, the reference inlet reservoir bottle
must contain a sufficient level of calibrant solution (>50 mL), and the waste bottle must not
be full.
The factory-supplied calibrant (compatible with the MSQ 2.0 software) contains 1.3 mM
sodium iodide (NaI), 0.05 M potassium iodide (KI), and 0.02 mM cesium iodide (CsI) in
50:50 isopropanol/water. A 250 mL bottle of calibrant is shipped with the MSQ Plus Mass
Detector. You can purchase refill bottles from Thermo Fisher Scientific or prepare your own
calibration solution. See “Calibrant Solution” on page 106 for a suggested procedure for
preparing the calibration solution.
Thermo Scientific
MSQ Plus Mass Detector Getting Started Guide
25
4 Calibrating the MSQ Plus Mass Detector
Preparing the MSQ Plus Mass Detector for Calibration
 To check the solvent levels in the reservoir bottles
1. Open the front door of the MSQ Plus Mass Detector by depressing the door latch on the
left side of the mass detector as you pull forward on the door.
2. Check the level of the calibrant solution in the reference inlet reservoir, which is the bottle
on the right. See Figure 11.
Figure 11. View of reference inlet reservoir bottle and waste bottle
Connected to
port 6 of valve
Connected to
port 5 of valve
Nitrogen
pressurization line
BOTTLE CAP
MUST BE
CLOSED
TIGHTLY
Clip
Clip
3. If the volume of calibrant solution is less than 50 mL, refill the bottle. After you refill the
bottle, ensure that the bottle cap is closed tightly.
4. Check the level of waste solution in the waste bottle, the bottle on the left (see Figure 11).
5. If the waste bottle is full of solvent, empty it.
Setting the Gas Flows and Probe Heater Temperature
Turn on the nitrogen gas and the probe heater.
 To turn on the nitrogen gas and the probe heater
1. Verify that the nitrogen regulator is set to 5.2 bar (75 psi). See Figure 5 on page 16.
2. Right-click the Server icon in the Windows taskbar, and choose Manual Tune, as shown
in Figure 12.
26
MSQ Plus Mass Detector Getting Started Guide
Thermo Scientific
4 Calibrating the MSQ Plus Mass Detector
Preparing the MSQ Plus Mass Detector for Calibration
Figure 12. Server shortcut menu
3. If it is not already open, open the Per Method Parameters table of the Tune window,
shown in Figure 13, by clicking the Show Per Method Parameters bar at the top right of
the Tune window.
Figure 13. View of Per Method Parameters table with Operate on and Nitrogen Gas on
4. Click the Nitrogen Gas On/Off toggle button to turn the nitrogen flow on.
The toggle button turns green, and the text changes to On. Within a few seconds, you
hear the flow of the nitrogen gas.
5. Click the Operate toggle button to place the system in the operate mode.
Note The gas flows are preset and do not require adjustment.
6. Click the Probe Temperature Setpoint box. Type 350 in the box, and then press
ENTER.
The probe temperature rises to 350 °C.
If the MSQ Plus Mass Detector has not been used recently or was last used in APCI mode, go
to the next topic, “Baking Out the Probe (Optional)” on page 28. If you do not need to bake
out the probe, go to “Flushing Air from the Lines of the Reference Inlet System” on page 28.
CAUTION Ensure that switching Operate to On also turns the Nitrogen Gas to On. High
probe temperature in the absence of nitrogen gas could damage the probe heater.
Thermo Scientific
MSQ Plus Mass Detector Getting Started Guide
27
4 Calibrating the MSQ Plus Mass Detector
Preparing the MSQ Plus Mass Detector for Calibration
Baking Out the Probe (Optional)
If the MSQ Plus Mass Detector has not been used recently or was last used in APCI mode,
bake out the probe before starting the LC flow. To do this, set the probe temperature to
550 °C and leave the nitrogen gas flowing for approximately 5 min. Then reset the probe
temperature to 350 °C.
Flushing Air from the Lines of the Reference Inlet System
Before you start the Instrument Tune and Calibration wizard, flush the air out of the tubing
that connects the reference inlet reservoir to the injection valve of the mass detector. Flushing
air out of the lines helps to ensure a successful calibration.
 To flush air out of the reference inlet system
1. Display the advanced parameters in the Per Method Parameters table:
a. From the menu bar of the Manual Tune window, choose View > Options, as shown
in Figure 14, to open the Tune Options dialog box.
Figure 14. View menu
b. Select the Show Advanced Parameters check box (see Figure 15). Then click OK to
exit the dialog box.
28
MSQ Plus Mass Detector Getting Started Guide
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4 Calibrating the MSQ Plus Mass Detector
Preparing the MSQ Plus Mass Detector for Calibration
Figure 15. Tune Options dialog box
2. In the Sequence Control area of the Per Method Parameters table, click the Inject from
Ref. Inlet toggle button. See Figure 16.
Figure 16. Per Method Parameters table with advanced parameters
Inject from Ref. Inlet
toggle button
The MSQ Plus Mass Detector draws calibrant from the reference inlet reservoir bottle
through the tubing and into 500 L sample loop for 35 seconds.
Thermo Scientific
MSQ Plus Mass Detector Getting Started Guide
29
4 Calibrating the MSQ Plus Mass Detector
Preparing the MSQ Plus Mass Detector for Calibration
The MSQ Plus Mass Detector is ready for calibration. To view a mass spectrum of the
calibrant solution before you calibrate your mass detector, go to the next topic,“Viewing the
Mass Spectrum of the Calibrant Solution (Optional).”
Viewing the Mass Spectrum of the Calibrant Solution (Optional)
You can optionally view the mass spectrum produced by infusing the calibrant solution.
 To view the mass spectrum of the calibrant
1. Open the Advanced Options version of the Per Method Parameters table. For instructions
on displaying the Advanced Options parameters, see “Flushing Air from the Lines of the
Reference Inlet System” on page 28.
2. If the nitrogen gas is off, turn it on by clicking the Nitrogen Gas On/Off toggle button.
3. If the MSQ Plus Mass Detector is not in Operate mode, start its operation by clicking the
Operate toggle button.
4. In the Per Method Parameters table, set the following parameters:
• If the probe temperature is not already set to 350 °C, click the Probe Temperature
Setpoint box, type 350, and then press ENTER.
• If the needle voltage is not already set to 3 kV, click the Needle (kV) Setpoint box,
type 3, and then press ENTER.
5. In the Full Scan Events table, shown in Figure 17, set the scan parameters so that you can
view the entire mass spectrum of the calibrant solution:
• Click the Mass box and type 1000.
• Click the Span box and type 1997.
• Click the Scan Time box and type 2.
These settings let you to view the entire mass spectrum of the calibrant solution. The
characteristic peaks in the mass spectrum of the calibrant solution for the MSQ Plus Mass
Detector are listed in Table 3.
Figure 17. Full Scan Events table
Table 3. Characteristic peaks in the mass spectrum of the calibrant solution (Sheet 1 of 2)
30
Peak #
Mass-to-charge ratio
Peak #
Mass-to-charge ratio
1
22.99
8
1072.25
2
38.96
9
1222.14
3
83.05
10
1372.04
MSQ Plus Mass Detector Getting Started Guide
Thermo Scientific
4
Calibrating the MSQ Plus Mass Detector
Performing a Full-System Autotune
Table 3. Characteristic peaks in the mass spectrum of the calibrant solution (Sheet 2 of 2)
Peak #
Mass-to-charge ratio
Peak #
Mass-to-charge ratio
4
132.91
11
1521.93
5
172.88
12
1671.83
6
472.67
13
1821.72
7
622.57
14
1971.61
6. Turn on the solvent flow from the LC pump. Ensure that the pump is set to deliver 50:50
acetonitrile/water at a flow rate of 200 L/min.
7. In the Sequence Control area of the Per Method Parameters table, click the Inject from
Ref. Inlet toggle button, shown in Figure 16 on page 29.
The MSQ Plus Mass Detector draws calibrant from the reference inlet reservoir bottle,
through the tubing, and into the 500 L sample loop for 35 seconds. At a flow rate of
200 L/min, it takes 2.5 minutes to empty the sample loop. During this time, you can
view a mass spectrum of the calibrant solution in the Peak Display area of the
Tune window, as shown in Figure 18.
Figure 18. Mass spectrum of calibrant solution with centroid peak format
Performing a Full-System Autotune
After you move your MSQ Plus Mass Detector to a new environment, perform maintenance
on the inner components of the source block, or reinstall or upgrade the Xcalibur software,
you must perform a full-system autotune.
Note Always perform a full-system autotune in ESI mode.
 To perform a full-system autotune
1. Ensure that you have prepared your mass detector for calibration, as described in
“Preparing the MSQ Plus Mass Detector for Calibration” on page 24.
Thermo Scientific
MSQ Plus Mass Detector Getting Started Guide
31
4 Calibrating the MSQ Plus Mass Detector
Performing a Full-System Autotune
2. Set the LC pump to deliver a solvent composition of 50:50 acetonitrile/water at a flow
rate of 0.2 mL/min.
3. Right-click the Server icon to display a shortcut menu, and then choose Instrument
Tune and Calibration, as shown in Figure 19.
Figure 19. Server shortcut menu
The Instrument Tuning and Calibration wizard shown in Figure 20 appears.
Figure 20. Instrument Tuning and Calibration wizard
4. Select the Full System Autotune option, and then click Next.
A series of messages inform you of the progress of the tuning and the calibration
procedure. If the tuning or calibration procedure fails, an error message appears.
5. To accept the results of the autotune, click Finish.
The software replaces the current tune and calibration files with the ones generated by the
autotune.
6. Click Print Report to print the calibration report. An autotune report for a full-system
autotune is shown in Figure 21.
32
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4
Calibrating the MSQ Plus Mass Detector
Performing a Full-System Autotune
Figure 21. Autotune report for a full-system autotune
AutoTune Report
Laboratory:
Instrument ID:
Serial Number:
m/z:
Width:
173.02
0.081
175
171
Filename:
Tune Date:
Report Date:
Product Evaluation
MSQ Plus
999999
m/z:
Width:
1070
1072.23
0.78
1074
m/z:
Width:
1970
1971.54
1.22
0.00
0.00
-6.32
-0.59
172.88
Slow Mass Shift 250.00 Da/S
6.24
0.00
172.88
22.99
-6.24 1821.72
172.88
1971.61
1971.61
Slow Calibration 250.00 Da/S
0.08
0.00
-0.08
1971.61
22.99
Fast Mass Shift 1000.00 Da/S
Fast LM Shift 1000.00 Da/S
0.53
0.59
0.00
0.00
-0.53
-0.59
22.99
Fast Mass Shift 1000.00 Da/S
0.50
472.67
0.00
172.88
-0.50
172.88
1971.61
1971.61
Fast Calibration 1000.00 Da/S
0.13
0.00
-0.13
22.99
Diagnostic Messages
Tune Parameters
Probe Temperature (oC)
Needle Voltage (kV)
Detector Voltage (V)
RF Lens Bias (V)
Ion Energy (V)
Ion Energy Ramp (mV/amu)
Low Mass Resolution
High Mass Resolution
Cone (V)
RF Frequency
Linearity Adjust
Low Mass Res Mid Point
High Mass Res Mid Point
Set Point
349
3.0
988
0.5
0.5
0.3
12.7
12.5
75
236755120
-0.05
55
14147
Actual
342
3.0
1023
0.5
n/a
n/a
n/a
n/a
74
n/a
n/a
n/a
n/a
Mass Calibration Results
Calibration Type
Missed Ref Peaks
Standard Deviation[max 0.2]
R.M.S. Error
Max Residual Error(m/z)[max 0.3]
Calibrated Range Min(m/z)
Calibrated Range Max(m/z)
Fast
Autotune
0
0.0509
0.0520
.0.1328
22.9898
1971.6149
Slow
Autotune
0
0.0436
0.0446
.00778
22.9898
1971.6149
1974
(Peak widths are measured at 10% height)
Slow
Mass
Da/S
Slow
LM Shift
Shift 250.00
250.00 Da/S
6.32
0.59
Calibration.msqreport
10/27/2004 8:31:28 AM
10/27/2004 8:45:58 AM
1971.61
Ref-Peak
22.99
38.96
83.05
132.91
172.88
472.67
622.57
772.46
922.36
1072.25
1222.14
1372.04
1521.93
1671.83
1821.72
1971.61
Search18.99
37.03
81.00
130.93
168.88
470.71
620.52
770.38
920.30
1070.25
1220.18
1370.08
1519.99
1669.88
1819.77
1969.64
From-To
26.99
41.03
85.00
134.93
176.88
474.74
624.54
774.44
924.36
1074.27
1224.18
1374.09
1523.99
1673.86
1823.73
1973.69
Real-Peak
23.05
38.92
83.07
132.90
172.92
472.63
622.49
772.41
922.36
1072.28
1222.18
1372.09
1521.99
1671.88
1821.75
1971.60
Mass-Shift
0.06
-0.05
0.03
0.00
0.04
-0.05
-0.08
-0.05
0.00
0.04
0.04
0.05
0.05
0.05
0.03
-0.02
Ref-Peak
22.99
38.96
83.05
132.91
172.88
472.67
622.57
772.46
922.36
1072.25
1222.14
1372.04
1521.93
1671.83
1821.72
1971.61
Search18.99
37.04
80.91
130.93
170.90
470.66
620.54
770.37
920.28
1070.24
1220.15
1370.01
1519.96
1669.86
1819.76
1969.61
From-To
26.99
41.04
84.91
134.93
174.90
474.66
624.54
774.37
924.28
1074.24
1224.15
1374.01
1523.96
1673.86
1823.76
1973.61
Real-Peak
23.06
38.83
83.07
132.92
172.87
472.65
622.47
772.39
922.35
1072.26
1222.12
1372.06
1521.97
1671.87
1821.71
1971.60
Mass-Shift
0.07
-0.13
0.02
0.02
-0.01
-0.03
-0.10
0.01
-0.07
-0.01
0.02
0.03
0.03
0.04
-0.01
-0.01
No Messages.
AutoTune Status: Pass
Reviewed By:
Thermo Scientific
Date:
MSQ Plus Mass Detector Getting Started Guide
33
4 Calibrating the MSQ Plus Mass Detector
Performing a Mass-Scale Calibration
Performing a Mass-Scale Calibration
Perform a mass-scale calibration every three to six months as part of a standard maintenance
program or if you notice a drift in the mass accuracy of the mass detector.
Note Always perform the mass-scale calibration procedure in ESI mode.
Before you perform a mass-scale calibration, prepare your system, as described in “Preparing
the MSQ Plus Mass Detector for Calibration” on page 24.
 To perform a mass-scale calibration
1. Set the LC pump to deliver a solvent composition of 50:50 acetonitrile/water at a flow
rate of 0.2 mL/min.
2. Right-click the Server icon to display the shortcut menu shown in Figure 19 on page 32,
and choose Instrument Tune and Calibration to open the Instrument Tuning and
Calibration wizard shown in Figure 20 on page 32 .
3. Select the Mass Scale Calibration option, and click Next.
A series of messages inform you of the progress of the calibration. If the calibration fails,
an error message appears.
4. To accept the results of the calibration, click Finish.
The current calibration file is replaced with the one generated by the mass-scale
calibration procedure.
5. Click Print Report to print the calibration report.
Performing a Detector Gain Calibration
Note Detector gain calibration is only available in the engineering version of the Tune
application.
As ions exit the mass analyzer, the gain of an electron multiplier is the number of electrons
created in each ion event. The gain depends on the age of the electron multiplier and the
velocity of the impinging electron which, in turn, depends on the voltage applied between the
input and output of the multiplier.
At a given point in the lifetime of an electron multiplier, a single ion event has a given gain. As
the electron multiplier ages, its gain decreases, because it does not release as many secondary
electrons from a given ion event. To maintain a gain of approximately 4.5  105, the voltage
applied across the input and output of the multiplier must be increased.
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4
Calibrating the MSQ Plus Mass Detector
Performing a Detector Gain Calibration
The change in gain over time is directly related to the number of ions that impinge the
detection system and the voltage at which the electron multiplier is operated. Under normal
operating conditions, this decrease in detector gain, which translates as a loss in sensitivity,
necessitates a detector gain calibration every three to four months. If the detector is subject to
heavy use, you might need to perform a detector gain calibration every two months.
You can automatically calibrate the gain of the electron multiplier by running the detector
gain calibration routine, as described in the following procedure.
 To calibrate the gain of the electron multiplier
1. Ensure that you have prepared your MSQ Plus Mass Detector for calibration, as described
in the “Preparing the MSQ Plus Mass Detector for Calibration” on page 24.
2. In the area of the Tune window, do the following:
• In the Peak Format list, select Profile.
• In the Mass box, type 300 or an equivalent value for the center mass corresponding
to a relatively low intensity peak <104 cps.
IMPORTANT The gain routine strives to achieve conditions where it is receiving
single ion counts. Therefore, it is important to scan a mass region where only
low-level chemical noise is present. Avoid placing the center mass at a high-intensity
peak. Doing so will make it harder for the software to control the ion flow rate and
cause the tuning process to fail.
3. Right-click the Status Indicator and choose Instrument Tune and Calibration to open
the Instrument Tuning and Calibration wizard. See Figure 19 on page 32.
4. Select the Detector Gain Calibration option, and then click Next.
The Detector Gain Calibration confirmation page appears.
5. Ensure that you set up the LC/MS system, as described in step 1 and step 2 of this
procedure, and click Next to start the automated calibration process.
During the automated calibration process, the software performs the steps listed in
Table 4.
Table 4. Steps in the automated calibration process (Sheet 1 of 2)
Automated Detector Gain Routine
Thermo Scientific
Step 1
Turns off the ion flow and captures the electronic noise.
Step 2
Determines the peak-to-peak difference between the minimum and
maximum values for the absolute analog to digital converter noise.
Step 3
Turns the ion flow on.
Step 4
Starting from 700 V, increases the voltage in 50 V intervals until the
peak-to-peak signal intensity is more than 10 times the noise signal.
MSQ Plus Mass Detector Getting Started Guide
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4 Calibrating the MSQ Plus Mass Detector
Performing a Detector Gain Calibration
Table 4. Steps in the automated calibration process (Sheet 2 of 2)
Automated Detector Gain Routine
Step 5
Adjusts the ion flow rate to approximately 1000 counts per second.
Measures the multiplier output response to each ion event to determine
gain at this multiplier voltage.
Step 6
Raises the multiplier voltage by 50 V and repeats step 5. This process is
repeated several times.
Step 7
Creates a calibration curve over the measured multiplier voltage range and
determines the multiplier voltage required to produce a gain of 450 000.
Once the tune process successfully finishes, the equation of the calibration curve appears,
and the Finish button becomes available as shown in Figure 22.
Figure 22. Instrument Tuning and Calibration wizard showing the calibration curve produced
by the tune process
The equation of the calibration curve produced by the tune event shown in Figure 22 is as
follows:
Voltage = –438 + 149 × ln(450 000)
Voltage = 1506
6. If the calibration has not finished within 12 minutes, click Cancel, and verify that the
value in the Mass box corresponds to a low-intensity region.
7. Click Finish.
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4
Calibrating the MSQ Plus Mass Detector
Performing a Detector Gain Calibration
The Confirm Set Electron Multiplier Voltage dialog box appears, as shown in Figure 23.
Figure 23. Confirm Set Electron Multiplier Voltage dialog box
WARNING To complete the detector gain calibration, do one of the following:
• To apply the new voltage setting to the electron multiplier, click OK. The new
voltage setting is stored in the registry.
–or–
• To keep the current detector voltage, click Cancel.
After you complete the detector gain calibration, the Tune page appears. As Figure 24
shows, the software automatically stores tune reports in the following directory for
Xcalibur 2.2 SP1or later:
Drive:\Thermo\Instruments\MSQ\Cali\report
Thermo Scientific
MSQ Plus Mass Detector Getting Started Guide
37
4 Calibrating the MSQ Plus Mass Detector
Performing a Detector Gain Calibration
Figure 24. Contents of Thermo\Instruments\MSQ\Cali\Report for 2.3
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MSQ Plus Mass Detector Getting Started Guide
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5
Manual Tuning in ESI Mode
Before you create an instrument method, you must optimize the acquisition parameters of the
MSQ Plus Mass Detector for your compound of interest. The process of optimizing the
acquisition parameters of the MSQ Plus Mass Detector for a particular compound or family
of compounds is referred to as manual tuning and is performed from the Tune window.
This chapter describes how to optimize the tune parameters for a compound of interest.
Perform the procedures that are provided in this chapter in the following order. If your LC
devices are controlled by the Xcalibur data system, begin with “Setting Up the LC for Direct
Control” on page 40. Otherwise, begin with “Selecting Your Tune Options” on page 41.
Contents
• Setting Up the LC for Direct Control
• Selecting Your Tune Options
• Acquiring and Viewing a Raw Data File
In the ESI mode, you optimize the parameters listed in Table 5.
Table 5. Tune controls in the ESI mode (Sheet 1 of 2)
Tune window control
Description
Typical value
Needle (kV)
Voltage that is placed on
the stainless steel insert
capillary.
The optimal value depends on the structure of your
analyte and the flow rate of the mobile phase.
Thermo Scientific
Typical values range from 3 to 5 kV.
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Manual Tuning in ESI Mode
Setting Up the LC for Direct Control
Table 5. Tune controls in the ESI mode (Sheet 2 of 2)
Tune window control
Description
Typical value
Probe Temperature
Temperature setting of the
probe heater.
The optimal value depends on the flow rate of the mobile
phase. In general, you must increase the probe
temperature as you increase the flow rate of the mobile
phase. See Table 1 on page 7.
Cone (V)
Voltage that is placed on
the source block and the
entrance cone.
The optimal value depends on the structure of your
analyte.
Increasing the cone voltage can decrease adduct
formation and give rise to fragmentation, which provides
structural information about your analyte.
Typical cone voltage values range from 75 to 150 V.
Note To enter an optimal needle voltage for your application into an instrument method,
you must empirically determine the optimal needle voltage from the Tune window, save
the value in a tune file, and then import the tune file into an instrument method.
If you are a novice user, you might want to experiment with the manual tuning features of the
MSQ Plus Mass Detector by starting with one of the tune files included with the data system.
For ESI mode, start with the esipos.tune file and inject the sensitivity test compound that is
shipped with your mass detector. For most analytes, the RF lens bias and ion energy
parameters are optimized at 1.0 V and 0.8 V, respectively. The suggested sensitivity test
solution for the positive-ion polarity mode is a 5 pg/μL solution of erythromycin. The
preparation of these test solutions is described in “Sample Formulations” on page 101.
If you are an advanced user, see the Help for information on the advanced options in the Tune
window.
Setting Up the LC for Direct Control
During the manual tuning process, you make several injections. If your LC system is
controlled from the Xcalibur data system, open the Instrument Setup window before you
open the Tune window so that you can access the Direct Control dialog boxes for the LC
pump and the autosampler.
 To open the Instrument Setup window and adjust its size as follows
1. For Xcalibur 2.2 SP1 or later, choose Start > Programs > Thermo Xcalibur > Xcalibur.
On the Roadmap view of the Xcalibur data system, click the Instrument Setup icon.
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Manual Tuning in ESI Mode
Selecting Your Tune Options
The Xcalibur data system displays the Instrument Setup window with icons for all of the
currently configured devices for your LC/MS instrument arranged down the left side of
the view. Instrument Setup pages and dialog boxes for the selected device are displayed on
the right side of the view. You can access additional dialog boxes for the device by clicking
the device listing in the menu bar.
2. Leave the Instrument Setup window open, but adjust its size to half the size of your view
screen or minimize it.
When you acquire data in the Tune window, use the Direct Control dialog box for the LC
pump to start the mobile phase flow. You use the Direct Control dialog box for the
autosampler to inject your analyte.
Selecting Your Tune Options
The display in the Tune window contains a mass spectrum corresponding to each row in the
Scan Events table at the bottom of the window. As you optimize the MS parameters for a
particular compound, the mass spectra displayed in the Tune window will change. To record
the changes in the mass spectra, collect the data in a raw data (.raw) file. If you are a novice
user, you might want to experiment with the tune parameters for your compound by starting
with one of the tune files that is shipped with the data system.
The following procedure describes how to create a file name for the raw (.raw) data file that is
created by the data system after you start data acquisition from the Tune window. It also
explains how to select a stored tune (.tune) file from the Tune window that you use as a
template.
 To select your tune options
1. Right-click the Server icon to display a shortcut menu, and choose Manual Tune to open
the Tune window.
2. Choose View > Options to open the Tune Options dialog box, shown in Figure 25.
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Manual Tuning in ESI Mode
Selecting Your Tune Options
Figure 25. Tune Options dialog box
Raw data file
button
Tune file button
3. Enter an acquisition file name for the raw data file that the data system creates after you
start data acquisition in the Tune window:
a. Click the button to the right of the Acquisition Filename box to open the Select Tune
Raw File Name dialog box, shown in Figure 26.
Figure 26. Select Tune Raw File Name dialog box
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Manual Tuning in ESI Mode
Selecting Your Tune Options
b. Browse to the directory where you want to store your raw data. Then type an
appropriate file name in the Filename box.
c. Click Open to create the file name for the raw file, and close the Select Tune Raw File
Name dialog box.
Note The Xcalibur data system does not check your directory for the reserved file
name. If you reuse a file name, the data system appends the date and time at
which you start data acquisition to the file name.
4. Open and download the parameters from a stored tune file:
a. Click the button to the right of the Tune Filename box to open the Select Tune
Parameter File Name dialog box, shown in Figure 27.
Figure 27. Select Tune Parameter File Name dialog box
b. Select an appropriate tune file or create a new tune file.
c. If you are a novice user and you want to experiment with the tune parameters, open
the esipos.tune file, as shown in Figure 28.
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Manual Tuning in ESI Mode
Acquiring and Viewing a Raw Data File
Figure 28. Displaying the tune parameters contained in the esipos.tune file in the Tune window
ESI mode
Positive
ion polarity
mode (+ve)
d. Click Open to download the tune parameters, and close the Select Tune Parameter
File Name dialog box.
Acquiring and Viewing a Raw Data File
Follow the steps in this section to acquire and view a raw data file.
 To acquire and view a raw data file
1. Start the mass detector:
a. If the Per Method Parameters table is not open, open it by clicking the Show Per
Method Parameters bar.
b. Turn on the nitrogen flow to the API probe by clicking the Nitrogen Gas toggle
button.
c. Start the operation of the MSQ Plus Mass Detector by clicking the Operate toggle
button.
2. Start the LC flow. Use the same mobile phase conditions that you plan to use when you
create your acquisition method.
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Acquiring and Viewing a Raw Data File
3. Start the acquisition of data by clicking the Start Acquiring icon, shown in Figure 29, in
the Tune window toolbar.
Figure 29. Start Acquiring and Stop Acquiring icons in the Tune window toolbar
Stop Acquiring button
Start Acquiring button
4. From the Roadmap view, double-click the Qual Browser icon,
Browser window, shown in Figure 30.
, to open the Qual
Figure 30. Qual Browser window
5. Open your raw data file:
a. From the Qual Browser menu bar, choose File > Open to open the Open Raw File
dialog box.
b. Select the raw data file that you are acquiring.
c. Click Open to open the file in the Qual Browser window, and close the Open Raw
File dialog box, shown in Figure 31.
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Acquiring and Viewing a Raw Data File
Figure 31. Open Raw File dialog box
6. Inject your sample. If your autosampler is controlled by the Xcalibur data system, open its
Direct Control dialog box from the Instrument Setup window. Enter an appropriate
injection volume and vial location. Click Apply.
7. From the Qual Browser window, observe the chromatogram. To view the data as it is
acquired, periodically press F5.
8. From the Tune window, change the parameters for the needle voltage, probe temperature,
and cone voltage.
You cannot alter the cone voltage while data acquisition is in progress.
Note When you create an instrument method, you import the tune file that contains
the optimized needle voltage for your application. You must manually enter the
optimal probe temperature and cone voltage into the instrument method.
9. Repeat step 6 through step 8 until you finish optimizing the parameters for your
compound.
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Acquiring and Viewing a Raw Data File
10. After you finish optimizing the parameters for your compound, stop the data acquisition
by clicking the Stop Acquiring icon shown in Figure 29 on page 45 in the Tune window
toolbar.
11. Save your tune file:
a. Choose File > Save As from the Tune window menu bar to open the Save Tune
Parameters dialog box, shown in Figure 32.
Figure 32. Save Tune Parameters dialog box
b. Type an appropriate name in the File name box.
c. Click Save to save the tune file with the optimized MS parameters for your
compound of interest.
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Manual Tuning in the APCI Mode
Before you create an instrument method, you must optimize the acquisition parameters of the
MSQ Plus Mass Detector for your compound of interest. The process of optimizing the
acquisition parameters of the mass detector for a particular compound or family of
compounds is referred to as manual tuning and is performed from the Tune window.
This chapter describes how to optimize the tune parameters for a compound of interest.
Perform the procedures that are provided in this chapter in the following order. If your LC
devices are controlled by the Xcalibur data system, begin with “Opening the Instrument
Setup Window” on page 51. Otherwise, begin with “Selecting Your Tune Options” on
page 51.
Contents
• Opening the Instrument Setup Window
• Selecting Your Tune Options
• Acquiring and Viewing a Raw Data File
In the APCI mode, you optimize the parameters listed in Table 6.
Table 6. Tune controls in the APCI mode (Sheet 1 of 2)
Tune window control
Description
Typical value
Corona (mA)
This is the current on the
corona pin.
The optimal value depends on the structure of your
analyte.
Although allowable values range from 2 to 50 uA, use the
lowest value that gives adequate sensitivity. Corona
readbacks might vary, depending on the eluent
composition and conductivity. Typical values range from
3 to 5 kV.
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Manual Tuning in the APCI Mode
Table 6. Tune controls in the APCI mode (Sheet 2 of 2)
Tune window control
Description
Typical value
Probe Temperature
This is the temperature
setting of the probe heater.
The optimal value depends on the flow rate of the mobile
phase. In general, you need to increase the probe
temperature as you increase the flow rate of the mobile
phase. See Table 1 on page 7.
Cone (V)
This is the voltage that is
placed on the source block
and the entrance cone.
The optimal value depends on the structure of your
analyte.
Increasing the cone voltage can decrease adduct
formation, common to ESI, and give rise to
fragmentation, which provides structural information
about your analyte. Increasing the cone voltage to reduce
adduct formation is useful in the presence of interfering
compounds.
Typical cone voltage values range from 75 to 150 V.
Note To enter the optimal corona current for your sample into an instrument method,
you must determine its value from the Tune window, save the value in a tune file, and
then import the Tune file into an instrument method.
The MSQ Plus Mass Detector is shipped with the ESI probe installed. Install the APCI probe
and the APCI corona pin before you begin the procedures that are provided in this chapter.
Figure 33 shows the APCI setup for the MSQ Plus Mass Detector. For instructions on
switching the probes, refer to the MSQ Plus Mass Detector Hardware Manual.
Figure 33. MSQ Plus Mass Detector setup for the APCI mode
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Manual Tuning in the APCI Mode
Opening the Instrument Setup Window
The corona pin position might be dropped by as much as 1 to 2 centimeters if it improves the
signal.
If you are a novice user, you might want to experiment with the manual tuning features of the
MSQ Plus Mass Detector by starting with one of the tune files included with the data system.
For the APCI mode, start with the apcipos.tune file and inject the sensitivity test compound
that is shipped with your mass detector. For most analytes, the RF lens bias and ion energy
parameters are optimized at 1.0 V and 0.8 V, respectively. The suggested sensitivity test
solution for the positive ion polarity mode is a 5 pg/μL solution of erythromycin. The
preparation of this test solution is described in “Sample Formulations” on page 101. If you are
an advanced user, see the Help for information on the advanced options in the Tune window.
Opening the Instrument Setup Window
If your LC system is controlled from the Xcalibur data system, open the Instrument Setup
window before you open the Tune window.
 To open the Instrument Setup window
1. For Xcalibur 2.2 SP1 or later, choose Start > Programs > Thermo Xcalibur > Xcalibur.
On the Roadmap view of the Xcalibur data system, click the Instrument Setup icon.
Xcalibur displays the Instrument Setup window with icons for all of the currently
configured devices for your LC/MS instrument arranged down the left side of the view.
Instrument Setup pages and dialog boxes for the selected device are displayed on the right
side of the view. You can access additional dialog boxes for the device by clicking the
device listing in the menu bar.
2. Leave the Instrument Setup window open, but adjust its size to half the size of your view
screen or minimize it.
When you acquire data in the Tune window, use the Direct Control dialog box for the LC
pump to start the mobile phase flow and the Direct Control dialog box for the
autosampler to inject your analyte.
Selecting Your Tune Options
The display section of the Tune window contains a mass spectrum for each row in the
Scan Events table at the bottom of the window. As you optimize the MS parameters for
particular compound, the intensities of the mass spectra displayed in the Tune window will
change. To record the changes in the mass spectra, collect the data in a raw data (.raw) file. If
you are a novice user, you might want to experiment with the tune parameters for your
compound by starting with one of the tune files that is shipped with the data system.
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Manual Tuning in the APCI Mode
Selecting Your Tune Options
The following procedure describes how to create a file name for the raw (.raw) data file that is
created by the data system after you start data acquisition from the Tune window. It also
explains how to select a stored tune (.tune) file from the Tune window that you use as a
template.
 To select your tune options
1. Right-click the Server icon to display its shortcut menu, and choose Manual Tune to
open the Tune window.
2. Choose View > Options to open the Tune Options dialog box, shown in Figure 34.
Figure 34. Tune Options dialog box
3. Enter an acquisition file name for the raw data file that will be created by the data system
after you start data acquisition in the Tune window:
a. Click the button to the right of the Acquisition File Name box to open the
Select Tune Raw File Name dialog box, shown in Figure 35.
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Manual Tuning in the APCI Mode
Selecting Your Tune Options
Figure 35. Select Tune Raw File Name dialog box
b. Browse to the directory where you want to store your raw data. Then type an
appropriate file name into the File Name box.
c. Click Open to create the file, and close the Select Tune Raw File Name dialog box.
4. Open and download the parameters from a stored tune file:
a. Click the button to the right of the Tune File Name box to open the Select Tune
Parameter File Name dialog box, shown in Figure 36.
Figure 36. Select Tune Parameter File Name dialog box
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Manual Tuning in the APCI Mode
Acquiring and Viewing a Raw Data File
b. Select an appropriate tune file or create a new tune file.
c. If you are a novice user and you want to experiment with the tune parameters, open
the apcipos.tune file, as shown in Figure 37. For most analytes, the RF lens bias and
ion energy parameters are optimized at 1.0 V and 0.8 V, respectively.
Figure 37. Displaying the tune parameters from the apcipos.tune file in the Tune window
APCI mode
Positive
ion polarity
mode (+ve)
d. Click Open to download the tune parameters and close the dialog box.
Acquiring and Viewing a Raw Data File
You can acquire a raw data file as you adjust the tune parameters for your compound of
interest.
 To acquire a raw data file as you adjust the tune parameters
1. Start the mass detector:
a. If the Per Method Parameters table is not open (see Figure 13 on page 27), open it by
clicking the Show Per Method Parameters bar.
b. Turn on the nitrogen flow to the API probe by clicking the Nitrogen Gas toggle
button.
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Manual Tuning in the APCI Mode
Acquiring and Viewing a Raw Data File
c. Start the operation of the MSQ Plus Mass Detector by clicking the Operate toggle
button.
2. Start the LC flow. Use the same mobile phase conditions that you plan to use when you
create your acquisition method.
3. Start the acquisition of data by clicking the Start Acquiring icon, shown in Figure 38, in
the Tune window toolbar.
Figure 38. Start Acquiring and Stop Acquiring icons in the Tune window toolbar
Stop Acquiring button
Start Acquiring button
4. Double-click the Qual Browser icon,
, to Open the Qual Browser window, shown in
Figure 30 on page 45, from the Roadmap view.
5. From the Qual Browser menu bar, choose File > Open to open the Open Raw File dialog
box. Select the raw data file that you are acquiring. Then, click Open to open the file in
the Qual Browser window.
6. Inject your sample. If your autosampler is controlled by the Xcalibur data system, open its
Direct Control dialog box and select an appropriate injection volume and vial location.
7. Observe the chromatogram in Qual Browser. To view the data as it is acquired,
periodically press F5.
8. Change the parameters for the corona current, probe temperature, and cone voltage in the
Tune window.
You cannot alter cone voltage while data acquisition is in progress.
Note When you create an instrument method, you import the tune file that contains
the optimized needle voltage for your application. You must manually enter the
optimal probe temperature and cone voltage into the instrument method.
9. Repeat step 6 to step 8 until you finish optimizing the parameters for your compound.
10. After you finish optimizing the parameters for your compound, stop the data acquisition
by clicking the Stop Acquiring icon, shown in Figure 38, in the Tune window toolbar.
11. Save your tune file:
a. Choose File > Save As from the Tune window menu bar to open the Save Tune
Parameters dialog box, shown in Figure 39.
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Manual Tuning in the APCI Mode
Acquiring and Viewing a Raw Data File
Figure 39. Save Tune Parameters dialog box
b. Type an appropriate name in the File name box.
c. Click Save to save the tune file with the optimized MS parameters for your
compound of interest.
Note Default tune files are read-only.
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7
Creating Instrument Methods
You create an instrument method from the Instrument Setup window in the Xcalibur data
system to automatically control the MSQ Plus Mass Detector and your LC devices during a
sequence run.
This chapter describes the features of the method editing windows for the MSQ Plus Mass
Detector and how to create an LC/MS instrument method that controls both your MSQ Plus
Mass Detector and your LC devices.
Contents
• Welcome Page
• Method Editor
• Creating an LC/MS Instrument Method
Welcome Page
From the Welcome page for the MSQ Plus Mass Detector, shown in Figure 40, you can
choose from a list of method templates, open existing method files, and access to the Tune
window.
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Creating Instrument Methods
Method Editor
Figure 40. MSQ Plus Mass Detector Welcome page in the Instrument Setup window
Method Editor
The MSQ Plus Mass Detector Method Editor contains the following views, tables, and
menus, which are described in this topic:
• Method Options View
• Chromatogram View
• Per Method Parameters Table
• Full Scan Events Table
• SIM Scan Events Table
• Menus
You cannot have the Method Options view and the Chromatogram view open
simultaneously, nor can you have the Full Scan Events and the SIM Scan Events tables open
simultaneously. The Method Options view and the Full Scan Events table are shown in
Figure 41. The Chromatogram view and the SIM Scan Events table are shown in Figure 42
on page 60.
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Creating Instrument Methods
Method Editor
Figure 41. Method Editor with menus, method options, Per Method Parameters table, and Full Scan Events table visible
Method options
Per Method
Parameters table
Menus
Hide Method Options
bar
Full Scan Events
table
Show SIM Scans bar
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Creating Instrument Methods
Method Editor
Figure 42. Method Editor with menus, Chromatogram view, Per Method Parameters table, and SIM Events table visible
Per Method
Parameters table
Chromatogram view
Show Method
Options bar
Menus
Show Full Scans bar
SIM Scan Events table
To switch between the Chromatogram view and the Method Options view, click the Hide
Method Options or the Show Method Options bar. To switch between the SIM Scan Events
table and the Full Scan Events table, click the Show SIM Scans or the Show Full Scans bar.
Method Options View
You can use the Method Options view shown in Figure 43 to do the following:
• Open an existing raw file so that you can preview a chromatogram.
• Use the settings in the current tune file or a specific tune file.
CAUTION The MSQ Plus Mass Detector stops acquiring data after completing all the
mass detector's scan events. However, if the Continue to End of LC check box shown in
Figure 41 on page 59 is enabled, the mass detector continues acquiring indefinitely or
until you manually stop it.
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Creating Instrument Methods
Method Editor
Chromatogram View
The Chromatogram view allows you to open a previously acquired raw file (a file with the
.raw file extension) and display its chromatogram. You can right-click the chromatogram to
display a spectrum view, which shows the most abundant ion at the selected retention time, as
shown in Figure 43. You can also use the information in the raw file to edit the retention
times for the scan events in your method.
Figure 43. Chromatogram view showing a mass spectrum displayed by right-clicking the chromatogram
Spectrum view
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Creating Instrument Methods
Method Editor
Per Method Parameters Table
You can use the Per Method Parameters table, shown in Figure 44, to select the ionization
mode (ESI or APCI) and set the probe temperature. Typical probe temperature settings range
from 350 to 650 °C, depending on the ionization mode and the flow rate of the mobile phase.
See Table 1 on page 7 and Table 2 on page 7.
If the Per Methods Parameters table is not displayed, click the Show Per Method Parameters
Table bar to display it.
Figure 44. Per Method Parameters table
Full Scan Events Table
Use the Full Scan Events table, shown in Figure 45, to define all the full-scan events that you
want to acquire in your method. The parameters available to you in the Full Scan Events table
are listed in Table 7 on page 69.
Figure 45. Full Scan Events table showing one full-scan event
To perform source fragmentation, you can create two or more identical scan events, and then
enter increasing cone voltages into the Cone (V) boxes. To perform polarity switching, you
can create two identical scan events: one with the +ve polarity, and the other with the -ve
polarity.
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Creating Instrument Methods
Method Editor
SIM Scan Events Table
Use the SIM Scan Events table shown in Figure 46 to monitor a limited number of
mass-to-charge ratios, characteristic of a target compound or set of compounds. The mass
analyzer switches between the selected mass-to-charge ratios, each value being monitored for a
period of time (called the dwell time) before moving on to the next event.
SIM (selected ion monitoring) scan events offer the following:
• Improved sensitivity because more time is spent monitoring the ions of interest rather
than scanning across the full mass range
• A wide and linear dynamic range without modification of the tuning parameters, which is
important in isotope dilution techniques that use co-eluting labeled standards and in the
analysis of trace components.
• Better definition of the chromatographic peaks because more data points can be recorded
across each peak
• Reduced raw file sizes compared to a full scan because SIM scans record only a limited
amount of data relating to the particular mass-to-charge ratio of interest
Figure 46 lists the parameters available in the SIM Scan Events table.
Figure 46. SIM Scan Events table
Menus
The Method Editor view has two menus, the Preview menu and the Scan menu, as shown in
Figure 47.
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Creating Instrument Methods
Method Editor
Figure 47. Method Editor menus
Preview Menu
The Preview menu contains the following commands: All Scans, Full Scans, SIM Scans, and
Parameters. With these commands, you can do the following:
• View scan events, defined in the Full Scan Events and SIM Scan Events tables, in the
Chromatogram view.
• View and print a report of the method parameters.
 To use the commands in the Preview menu
• Choose All Scans to display scan events, defined in both the Full Scan Events table and
the SIM Scan Events table, in the Chromatogram view.
A colored bar appears for each scan event; a full scan event is orange and a SIM scan event
is green. If you hover the cursor over a bar, a tooltip showing the event name, start time,
and end time appears.
• Choose Full Scans to display scan events, defined in the Full Scan Events table, in the
Chromatogram view. Each scan event is displayed as an orange bar. If you hover the
cursor over a bar, a tooltip showing the event name, start time, and end time appears.
• Choose SIM Scans to display scan events, defined in the SIM Scan Events table, in the
Chromatogram view. Each scan event is displayed as a green bar. If you hover the cursor
over a bar, a tooltip showing the event name, start time and end time appears.
• Choose Parameters to display a report of the method parameters. Click Print to print the
report, or click Back to return to the Method Editor.
Scan Menu
The Scan menu contains the Add Full, Add SIM, Create Group, and Ungroup commands.
With these commands, you can do the following:
• Add and delete full and SIM scan events.
• Group and ungroup retention time ranges.
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Creating an LC/MS Instrument Method
 To use the commands in the Scan menu
• To add a full-scan event to the Full Scan Events table, choose Add Full. If you select a
full-scan event, the new scan uses the values of the selected event. If do not select a scan,
the values of the last row in the table are used.
Note Selected table rows are highlighted in green.
• To add a SIM scan event to the SIM Scan Events table, choose Add SIM. If you select a
SIM scan event, the new scan uses the values of the selected event. If you do not select a
scan, the values of the last row in the table are used.
• To create a group for a particular time range, click the first SIM event that you want to
add to the group. Pressing CTRL, click the events that you want to add. After you select
the events that you want to group, choose Create Group. The group uses the time range
and the polarity values listed in the first event. You can edit the time range and polarity
parameters for the group.
Tip Before an individual SIM event or group of SIM events is performed, the mass
detector performs a prefilter pulse to determine the baseline. To reduce the amount of
time spent on performing prefilter pulses, group events that use the same time range,
polarity, or both.
• To ungroup the SIM events, choose Ungroup. If the individual events initially contained
different settings for the time range, polarity, or both, they now contain the same settings
as the group.
• To delete a scan event or a group, click the event or the group to select it, and then choose
Delete.
Creating an LC/MS Instrument Method
To create an instrument method, follow the procedures in this section.
Opening the Instrument Setup Window
Opening the Instrument Setup window is the first step in creating an instrument method.
 To open the Instrument Setup window
1. For Xcalibur 2.2 SP1 or later choose Start > Programs > Thermo Xcalibur > Xcalibur.
In the Roadmap view of the Xcalibur data system, click the Instrument Setup icon.
Specifying the Method Parameters for the MSQ Plus Mass Detector
To enter the instrument control parameters for the MSQ Plus Mass Detector, you can select a
template or an existing method, or use the Method Editor to edit the method.
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Creating Instrument Methods
Creating an LC/MS Instrument Method
Selecting a Template or an Existing Method
You can select a template or an existing method from the Welcome page.
 To open the Welcome page and choose from among its options
1. Click the MSQ Plus button in the View bar of the Instrument Setup window to display
the Welcome page, shown in Figure 48.
The View bar is a vertical bar on the left of the Instrument Setup window. It contains
buttons for each of the instruments that you selected by using the Instrument
Configuration program.
Figure 48. Welcome page for the MSQ Plus Mass Detector
2. Select one of the options available to you:
• To create a new method, click a template for the type of acquisition that you want to
perform. The Method Editor opens with the default parameters for the template.
• To edit an existing method, click the following link to display the Open dialog box:
Open an existing file.
In the Open dialog box, select a method, and click Open. Click the MSQ Plus
button in the view bar of the Instrument Setup window to display the method in the
Method Editor.
• To open the Tune window, click the following link:
Access to tune allowing you to optimize or modify the system settings.
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Creating an LC/MS Instrument Method
Using the Method Editor to Edit the Method
To edit the method for the MSQ Plus Mass Detector, follow these procedures:
• Selecting the Method Options
• Selecting the Per Method Parameters
• Selecting the Scan Events
• Editing the Scan Events
Selecting the Method Options
Select the method options in the Method Editor view.
 To select the method options in the Method Editor view
1. From the Method Editor, click the Show Method Options bar to open the Method
Options view shown, in Figure 49.
Figure 49. Method Editor with the Method Options view
2. If you cannot see the browse buttons in the Method Options view, click the Hide Per
Method Parameters bar to hide the Per Method Parameters table.
3. (Optional) Open a raw file and display its chromatogram:
a. Click the browse button next to the Rawfile box.
b. From the Open dialog box, select a raw (.raw) file.
c. Click Open to close the Open dialog box and open the raw file.
d. Click the Hide Method Options bar to display the Chromatogram view.
You can use the chromatogram to edit the retention times of your scan events, as
described in “Editing the Scan Events” on page 69.
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Creating an LC/MS Instrument Method
e. After you finish working the chromatogram, click the Show Method Options bar to
reopen the Method Option view.
4. Select a tune (.tune) file by doing one of the following:
• Select the Use Current Tune File check box to use the Tune file that is in the current
memory of the Xcalibur data system.
• Clear the Use Current Tune File check box. Click the browse button next to the Use
Selected Tune file box to display the Open dialog box. Select the Tune file that you
created for your analyte, and then click Open.
Note For ESI methods, the instrument method uses the needle voltage value
from the Tune file. For APCI methods, the instrument method uses the corona
current value from the Tune file.
5. To stop the acquisition after completing all the mass detector's scan events, clear the
Continue to End of LC check box, shown in Figure 41 on page 59.
Selecting the Per Method Parameters
The “per method” parameters for the MSQ Plus Mass Detector are the ionization mode and
the probe temperature.
 To select the ionization mode and enter the probe temperature
1. If the Per Method Parameters table is not displayed, open it by clicking the Show Per
Method Parameters bar.
2. If you want to change the ionization mode, click the Ionization Mode Setpoint, and
select an appropriate ionization mode for your application.
3. To change the probe temperature, click the Probe Temperature Setpoint box. Type an
appropriate probe temperature in the box, and then press ENTER.
Note The probe temperature setting is not imported with the tune file. Use the
temperature that you determined empirically when you optimized the tune
parameters for your analytes.
Selecting the Scan Events
Table 7 lists the allowable ranges for the parameters in the Full Scan Events table. Table 8 lists
the allowable ranges for the parameters in the SIM Scan Events table.
Your method can contain both SIM scan events and full-scan events.
• Use SIM (selected ion monitoring) scan events for trace analysis and quantitation.
• Use full-scan events for rapid screening and qualitative analysis.
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Table 7. Full Scan Events table parameters
Parameter
Range
Entry
Name
Alphanumeric characters
Click the Name box and type a name consisting of alphanumeric
characters.
Mass Range
1.00–2000.00
Click the Mass Range box and type the beginning mass, a hyphen,
and the ending mass.
Time Range
0.00–9999.00
Click the Time Range box and type the beginning time point, a
hyphen, and the ending time point.
Peak Format
Centroid or Profile
Click the Peak Format list and select Centroid or Profile from the list.
Scan Time
0.01 to 8.00
Click the Scan Time box and type a length of time for the scan.
Range: 0.25–1.0
Polarity
+ve or -ve
Click the Polarity list and select +ve or -ve from the list.
Cone (V)
0.00 to 200.00
(typical 70.00 to 150.00)
Click the Cone (V) box and type the value of the optimal cone
voltage for your application.
Table 8. SIM Scan Events table parameters
Parameter
Range
Entering
Name
Alphanumeric characters
Click the Name box and type a name consisting of alphanumeric
characters.
Mass
1.00–2000.00
Click the Mass Range box and type the m/z ratio of the ion that you
want to monitor.
Span
0.00 to 1998
Click the Span box and type an appropriate span (m/z). For SIM scan
events, typical values range between 0.05 and 0.5.
Time Range
0.00–9999.00
Click the Time Range box and type the beginning time point, a
hyphen, and the ending time point.
Dwell Time
0.01 to 8.00
Click the Scan Time box and type a length of time for the scan.
Range: 0.05–0.25
Polarity
+ ve or –ve
Click the Polarity list and select +ve or –ve from the list.
Cone (V)
0.00 to 200.00
(typical 70.00 to 150.00)
Click the Cone (V) box and type the value of the optimal cone
voltage for your application.
Editing the Scan Events
You can manually edit scan events, as described in Table 7 and Table 8, or you can edit scan
events by using the information stored in a previously acquired raw data file.
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Creating an LC/MS Instrument Method
 To change the start or end time of an event with the Chromatogram view
1. Click a scan event in either the Full Scan Events or the SIM Scan Events table to select it.
2. Place the cursor at either end of the colored bar:
• If your cursor is at the left end of the bar, it will change to a right arrow
.
• If your cursor is at the right end of the bar, it will change to a left arrow
.
3. Drag the cursor to the retention time that you want.
A pop-up window appears, showing the event name, start time, end time, and duration to
assist in the positioning of the cursor. The new values are automatically updated in the
Time Range column of the events table, as shown in Figure 50.
Figure 50. Pop-up window that appears when you adjust the start or end time of a scan event
4. To move the scan window of an event, place the cursor on the colored bar until this icon,
, appears.
5. Drag the bar to the retention time window that you want.
A pop-up window appears, showing the event name, start time, end time, and duration to
assist in the positioning of the bar. The new values are automatically updated in the Time
Range column of the events table.
Specifying the Method Parameters for the LC System
In the Instrument Setup view, icons for all of the currently configured instrument
components appear on the left side of the view. Instrument Setup pages and dialog boxes for
the selected instrument component appear on the right side of the view.
 To create an instrument method that controls your LC system
1. Select the device that you want to set up by clicking its icon in the View bar.
The Xcalibur data system displays the Instrument Setup view for the instrument that you
selected. This view can consist of one or more pages or dialog boxes.
2. Select the Instrument Setup options that are appropriate for your application.
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Creating an LC/MS Instrument Method
Saving the Method
You can save the instrument method in a file with a .meth file name extension.
 To save the instrument method as a METH file
1. Choose File > Save As to open the Save As dialog box, shown in Figure 51.
Figure 51. Save As dialog box
2. Browse through the directory tree to find the Drive:\Xcalibur\methods directory.
3. Type a file name in the File Name box.
4. Click Save to open the File Summary Information dialog box, shown in Figure 52.
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Figure 52. File Summary Information dialog box
5. Type a description of the Method file in the Description box.
6. Click OK to open the File Save – Audit Trail dialog box, shown in Figure 53.
Figure 53. File Save – Audit Trail dialog box
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Creating an LC/MS Instrument Method
7. Type a comment concerning the changes you made to the instrument method into the
Comment box.
8. Click Continue to close the File Save – Audit Trail dialog box and save the
Instrument Method.
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Creating and Running Sequences
This chapter describes how to acquire and view real-time data for a single sample.
Contents
• Creating a Single Sample Sequence
• Starting Data Acquisition
• Viewing the Data as It Is Acquired
Creating a Single Sample Sequence
To set up a sequence to inject a single sample, open the Sequence Setup window, create the
sequence, and save the sequence.
Opening Sequence Setup Window
From the Sequence Setup view, you can open the New Sequence Template dialog box.
 To open the New Sequence Template dialog box
1. Click the Sequence Setup icon,
Sequence Setup view.
, on the Xcalibur Roadmap view to open the
A view similar to that shown in Figure 54 appears.
Figure 54. Sequence Setup view
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Creating a Single Sample Sequence
2. From the Sequence Setup view, choose File > New.
A New Sequence Template dialog box similar to the one shown in Figure 55 appears. The
tray type and vial selections depend on the configuration of the autosampler.
Figure 55. New Sequence Template dialog box for an LC/MS with an Accela autosampler
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Creating a Single Sample Sequence
Creating the Sequence
Follow these steps to create a new sequence.
 To create a new sequence
1. Set the parameters in the General area:
a. Type a name for the raw data file into the Base File Name box.
b. Browse to the data file directory where you want to store your raw data files.
The data system adds the .raw file extension to the data files that contain the
chromatographic and spectral data.
c. Browse to the instrument method that you want to use to acquire your raw data files.
Instrument methods have a .meth file extension. For information on creating an
instrument method, see the Help. For more detailed information on controlling the
Accela LC system, refer to your manual.
d. If you have not yet created a processing method that contains the information needed
to quantify your unknowns, leave the Processing Method box blank.
You can create a processing method and reprocess your stored data files at a later date.
Processing methods have a .pmd file extension.
2. Set the parameters in the Samples area:
a. Type 1 in the Number Of Samples box.
b. Type 1 in the Number Of Injections box.
c. Type the vial position in the Initial Vial Location box.
d. Type an identifying name for the sample in the Base Sample ID text box.
e. Labeling a sample with a base sample ID is optional. If you do not enter a base
sample ID, the data system automatically uses the vial position as the base sample ID.
If you enter a base sample ID, the data system automatically appends the vial position
to your entry.
3. Click OK to open your sequence spreadsheet, as shown in Figure 56.
Figure 56. Sequence Setup view, showing a newly created one-line sequence
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Creating a Single Sample Sequence
The injection volume displayed in the Inj Vol column matches the injection volume
contained in your instrument method. You can override this injection volume value.
4. If you want to change the injection volume, double-click the spreadsheet cell containing
the injection volume value that you want to change, highlight the current value, and type
a new value in the cell. You can use the Column Arrangement icon,
, to make the
column cell visible.
For details on the parameters in the New Sequence Template dialog box, refer to the
Xcalibur Help or the following documents:
• For the Xcalibur 2.2 SP1 or later data system, see the Xcalibur Help or the Thermo
Xcalibur Data Acquisition and Processing User Guide.
5. To alter the current column arrangement, click the Column Arrangement toolbar
button to display the Column Arrangement dialog box, shown in Figure 57.
• To add a column to the sequence, select the column from the Available Columns list
and click Add.
• To remove a column from the sequence, select the column from the Displayed
Columns list and click Remove.
• To alter the position of the columns in the sequence, select the column from the
Displayed Columns list, and click either Move Up or Move Down as appropriate.
Figure 57. Column Arrangement dialog box
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Creating a Single Sample Sequence
Saving the Sequence
Save the sequence by following this procedure.
 To save the sequence
1. Choose File > Save As.
The File Summary Information dialog box appears, as shown in Figure 58.
Figure 58. File Summary Information dialog box
2. Type an appropriate description in the Description box, and click OK.
The Save As dialog box appears, as shown in Figure 59.
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Starting Data Acquisition
Figure 59. Save As dialog box
3. Browse to the appropriate folder where you want to save the sequence.
4. Type a file name in the File Name box.
5. Click Save.
Starting Data Acquisition
To inject a sample and start data acquisition, open a sequence file, select the row that you
want to run, select the run options, and start the run.
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Creating and Running Sequences
Starting Data Acquisition
Opening a Sequence File
Start data acquisition by opening a sequence file containing the sample that you want to run.
 To open a sequence file
1. From the Sequence Setup view in the Xcalibur Roadmap view, choose File > Open to
display the Open dialog box, shown in Figure 60.
Figure 60. Open dialog box showing the selection of a sequence file
2. Browse to the appropriate folder.
3. Select the sequence that contains the sample you want to run.
4. Sequence files are identified by their .sld file extension.
5. Click Open.
The Xcalibur data system opens the selected sequence.
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Starting Data Acquisition
Selecting the Rows That You Want to Run
Next, select the rows that you want to run.
 To select the row or rows that you want to run
1. Highlight the sequence row or rows that you want to run, even if the sequence contains
just one row. See Figure 61.
Figure 61. Sequence Setup view showing sequence with first row selected
2. Confirm that you have vials in the positions specified in the sequence rows that you plan
to run.
Selecting the Run Options and Starting the Run
Follow these steps to select the run options and start the run.
 To select the run options and start the run
1. From the toolbar, click the Run Sample icon,
shown in Figure 62.
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Starting Data Acquisition
Figure 62. Run Sequence dialog box
The User box contains your login name, and the Run Rows box contains the number of
the row that you selected in the sequence spreadsheet.
2. In the Acquisition Options area, confirm the following:
• The mass detector and LC components are configured for operation as Xcalibur
devices in the Instrument list.
• The autosampler is configured as the start instrument.
After the injection valve of the autosampler switches to the inject position, the
autosampler sends a signal to the detector to begin data collection.
3. To change the components of your LC system or change the device that starts the run, do
the following:
a. Click Change Instruments to open the Change Instruments In Use dialog box,
shown in Figure 63.
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Starting Data Acquisition
Figure 63. Change Instruments In Use dialog box
A list of components that have been configured for operation as Xcalibur devices
appears. The autosampler is typically selected as the start instrument.
b. Add or remove components as appropriate.
c. To select a start instrument in the Instrument list, click the Start Instrument list in
the row of the instrument of choice. The blank space changes to display “Yes.”
4. To start the run automatically, select the Start When Ready check box.
The Xcalibur data system starts the run after you click OK in the Run Sequence
dialog box. If your system contains an Accela pump, the run begins after the pump sends
a pump ready signal to the Accela autosampler. The Accela pump is not ready until it
monitors a stable backpressure, as defined in the instrument method.
Note To manually start an acquisition, clear the Start When Ready check box and
choose Actions > Start Analysis from the Sequence Setup menu. To subsequently
control the acquisition, choose Actions > Pause Analysis or Actions > Stop
Analysis.
5. In the After Sequence Set System area, make the following selections:
• Select the On option if you want Xcalibur to leave the devices in the On state after
the sequence ends.
• Select the Off option if you want Xcalibur to turn off the pump flow and place the
mass detector in the Off mode after the sequence ends. In the Off mode, the nitrogen
gas, probe heater, and high voltages are turned off. If your instrument contains a UV
detector, Xcalibur turns off the detector’s lamps.
• Select the Standby option if you want to the LC pump flow to stop after the
sequence ends.
Note The MSQ Plus Mass Detector application has no standby mode. If you are
actively developing an instrument method, leave the system on. Otherwise, turn
the system off at the end of a sequence to save nitrogen gas. The probe heater will
reach equilibrium temperature in 1 to 2 minutes when you turn the system back
on.
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Viewing the Data as It Is Acquired
6. Leave the parameters in the other areas at their defaults.
Use the Instrument Method and Programs areas in the Run Sequence dialog box to
specify particular acquisition or processing requirements. For details on these parameters,
see the Help or Thermo Xcalibur Acquisition and Processing User Guide.
7. Click OK to start the run.
Viewing the Data as It Is Acquired
You can view the data as it is acquired by using the real-time plot facilities.
 To view the data as it is acquired using the real-time plot facilities
1. Click the Real Time Plot View icon,
, on the Roadmap view toolbar.
The screen is automatically locked so you can view the progress of your run in real time.
2. If you want to freeze the run data, click the Unlocked icon,
.
When the screen is unlocked, you cannot monitor the real-time progress of your run, but
you can review your data. For example, you can display the spectrum for a particular peak
that has already eluted. Data collection continues off screen as you review your data.
3. To relock the display, click the Locked icon,
.
Reviewing Real-Time Data
Follow this procedure to review the data as it is being collected.
 To review the data as it is being collected
1. Unlock the display by clicking the Locked icon,
.
After you unlock the display, data collection continues off screen.
2. Pin the Mass Spectrum cell by clicking the pin icon,
the cell.
, in the upper right corner of
The pin in the upper right corner of the Mass Spectrum cell turns green. Cursor actions
in other cells, such as the chromatogram cell, now affect the view displayed in the
Spectrum cell.
3. Click the peak of interest in the Chromatogram cell.
In the Mass Spectrum cell, a mass spectrum appears for the time point that you
clicked on.
4. To resume monitoring real-time data acquisition, click the Locked icon,
lock the display.
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Viewing the Data as It Is Acquired
5. Pin the Chromatogram cell by clicking the pin in the upper right corner of the cell.
The pin in the upper right corner of the Chromatogram cell turns green. Cursor actions
in other cells, such as the Spectrum cell, now affect the view displayed in the
Chromatogram cell.
6. Click the m/z value of interest in the Mass Spectrum cell.
In the Chromatogram cell that contained the total ion chromatogram (TIC), a
chromatogram appears for the m/z value that you clicked.
7. To resume monitoring real-time data acquisition, click the Locked icon,
.
Adding Cells to the Display
You can display multiple cells in the real-time display view.
 To display multiple chromatogram cells
1. Click the Chromatogram cell to make it the active cell with a gray border.
2. Choose View > Ranges to open the Chromatogram Ranges dialog box, as shown in
Figure 64.
Figure 64. Chromatogram Ranges dialog box
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3. For each cell that you want to add, do the following:
a. Select a Type check box.
b. From the Detector list, select a detector.
c. From the Plot Type list, select a plot type.
4. Click OK to close the Chromatogram Ranges dialog box.
5. Choose View > Lock Display to resume monitoring real-time data acquisition.
Figure 65 shows the four traces resulting from the settings in the Chromatogram Ranges
dialog box:
1. The total ion chromatogram (TIC)
2. The extracted ion chromatogram for the mass range: 180 to 220 m/z
3. Base peak chromatogram for the masses: 182 and 213 m/z
4. UV chromatogram at 260 nanometers
Figure 65. Four traces generated by settings in the Chromatogram Ranges dialog box
1
2
3
4
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Using Qual Browser
This chapter provides an introduction to using Qual Browser, a facility that you can use to
review the MS data contained in your data files.
Contents
• Cell States
• Cursor Actions
• Opening a Raw Data File in Qual Browser
• Adding Cells to the Grid
• Viewing Reports
• Viewing the Mass Spectrum for a Specific Time Point
Cell States
When you open a raw data file in the Qual Browser window, the information within the data
file is displayed as a grid of cells. There are three hierarchical states, which are described in this
topic, for a cell within the grid:
• Inactive Cells
• Active but Unpinned Cells
• Active and Pinned Cells
The grid always contains either one active but unpinned cell or one pinned cell. If the grid
contains more than one cell, only one cell can be active, and the rest of the cells are inactive.
An unpinned cell has a gray pin icon,
A pinned cell has a green pin icon,
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Cell States
Inactive Cells
Inactive cells are not highlighted with a gray border, and the pin icon in their upper right
corners are gray. The cell in the lower portion of Figure 66 is inactive, as indicated by the
absence of a gray border. Menu commands, toolbar buttons, and cursor actions do not affect
inactive cells. If you want to zoom the contents of a cell or access its shortcut menu, you must
make it an active cell.
Active but Unpinned Cells
An active but unpinned cell is highlighted with a gray border, and the pin icon in its upper
right corner is gray. The cell in the upper portion of Figure 66 is active but unpinned. Menu
commands, toolbar buttons, and cursor actions affect the active cell. If no cell in the grid is
pinned, clicking an inactive cell in the grid makes it the active cell.
Active and Pinned Cells
Clicking a pin in the upper right corner of a cell makes it the pinned cell within the grid. A
pinned cell is an active cell that cannot be made inactive by clicking in another cell. Instead,
actions performed in the inactive cells affect the pinned cell, as described in “Cursor Actions”
on page 92. The lower cell in Figure 67 is a pinned cell.
If you want to automatically change the range of a cell by clicking in the grid, you must pin
the cell. For example, if you want to display the spectrum for the 1 minute time point without
opening the Spectrum Ranges dialog box, pin the Spectrum cell. Then, click the 1 minute
time point in the inactive chromatogram cell.
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Cell States
Figure 66. Qual Browser window displaying an active TIC chromatogram cell and an inactive mass spectrum cell
Gray border
Active cell
Unpinned
Inactive cell
Figure 67. Qual Browser window displaying an inactive cell and a pinned cell
Inactive cell
Pinned cell
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Cursor Actions
Cursor Actions
Within the cells of the grid, you can use the cursor in three ways:
• Click in the cell to select a point.
• Drag a line parallel to any axis to select a range.
• Drag a line in any diagonal direction to select an area.
The effect of these actions depends on the state of the cell. Within an active cell, cursor
actions rescale the plot in the ways shown in Table 9. If one of the cells is pinned, the cursor
action in any of the inactive cells is always applied to the pinned cell, as shown in Table 10.
Table 9. Effect of cursor action in an active cell
Cursor action
Effect
Drag parallel to X axis
Rescale graph showing selected x range only, same y range.
Drag parallel to Y axis
Rescale graph showing selected y range only, same x range.
Dragged area
Rescale graph showing both the selected x and y ranges.
Table 10. Effect of cursor action in an inactive cell on the pinned cell
Pinned cell
Cursor action
Effect
Spectrum
Click in a chromatogram cell
The spectrum cell displays the
spectrum at that retention time.
Chromatogram
Click in a spectrum cell
The chromatogram cell displays
chromatogram for the wavelength
selected in the spectrum cell.
Opening a Raw Data File in Qual Browser
The data files containing the raw chromatographic and spectral data have the .raw file
extension.
 To open a raw data file in Qual Browser
1. Click the Qual Browser icon on the Xcalibur Roadmap view, or choose Go To > Qual
Browser to display the empty Qual Browser window, shown in Figure 68.
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Opening a Raw Data File in Qual Browser
Figure 68. The empty Qual Browser window in the Xcalibur data system
2. Choose File > Open to display the Open Raw File dialog box, shown in Figure 69.
Figure 69. Open Raw File dialog box
3. Select the raw data file that contains the data for the sample that you want to work on.
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Adding Cells to the Grid
4. Select the window layout from the list at the bottom of the dialog box:
• Select Current Layout if the current layout for the Qual Browser window is different
from the default layout and you want to apply it to your data file.
• Otherwise, leave the window layout selection at its default of Default Layout.
5. Click Open to open the raw data file into the Qual Browser window.
Adding Cells to the Grid
You can add cells to the grid in Qual Browser.
 To add cells to the grid in Qual Browser
1. Open a raw data file in Qual Browser.
2. From the Qual Browser menu bar, choose Grid > Insert Cells.
3. Choose Left, Right, Above, or Below.
4. Right-click the cell that you just inserted into the grid to access a shortcut menu, as
shown in Figure 70.
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Viewing Reports
Figure 70. View of Qual Browser window with the shortcut menus for selecting the options for a cell
5. From the shortcut menu, choose the view that you want for this cell.
Viewing Reports
After you open a data file, you might want to check the instrument parameters that were used
to acquire it.
 To view a report that lists the instrument control parameters used to acquire the
data file
1. Open a raw data file in the Qual Browser facility, as described in “Opening a Raw Data
File in Qual Browser” on page 92.
2. Choose View > Report > Instrument Method to display the Instrument Method report.
The instrument method is displayed in the pinned cell. The instrument method is
divided by device, with the parameters for each device displayed on a separate page.
Figure 71 shows an Instrument Method report.
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Viewing Reports
Figure 71. Report for an instrument method
3. Click the Show Previous and Show Next buttons,
of your acquisition method.
, to move through the pages
4. Choose View > Report > Status Log to display the Status Log report.
The status log displays the settings that were imported from the tune file, as shown in
Figure 72.
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Viewing Reports
Figure 72. Status log showing needle voltage
5. Choose View > Report > Tune Method to display the Tune Method report.
The Tune Method report displays the settings that were determined during the
full-system autotune procedure, as shown in Figure 73.
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Viewing the Mass Spectrum for a Specific Time Point
Figure 73. Report for autotune settings
The non-zero and non-unity values for the three coefficients indicate that a successful
autotune has been stored on this system.
A clean installation or a reinstallation of the software might reset the RF Frequency, HM Res
Mid Point, and Detector Voltage parameters to default values. For optimized settings, refer to
a previously successful autotune report.
Viewing the Mass Spectrum for a Specific Time Point
You can view a mass spectrum for a specific time point in the chromatogram.
 To view a spectrum for a specific time point in the chromatogram
1. Open a raw data file into the Qual Browser facility.
2. If the grid does not contain both a Chromatogram cell and a Spectrum cell, add the
appropriate cell to the grid, as described in “Adding Cells to the Grid” on page 94.
3. Pin the Spectrum cell.
4. In the Chromatogram cell, click a time point that you are interested in.
The mass spectrum for the selected time point appears in the Spectrum cell, as shown in
Figure 74.
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9 Using Qual Browser
Viewing the Mass Spectrum for a Specific Time Point
Figure 74. Qual Browser window displaying the mass spectrum for the 3.76 minute time point
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A
Sample Formulations
This appendix provides instructions for preparing the sensitivity testing solutions and the
calibrant solution. A Thermo Fisher Scientific field service engineer uses the 5 pg/μL solution
of erythromycin to perform the sensitivity tests for the positive ion ESI and APCI modes and
the 2 pg/μL solution of p-nitrophenol to perform the sensitivity tests for the negative ion ESI
and APCI modes. The calibrant solution for systems running on the MSQ 2.0 software
version contains the reference compounds [(CsI, KI, and NaI in 50:50 isopropanol/water
(v/v)] that are used to perform a full-system autotune and a mass-scale calibration.
Contents
• Sensitivity Test Solutions
• Preparing the MSQ Plus Mass Detector Calibration Solution
• Calibrant Solution
CAUTION Always take safety precautions when you handle chemicals and unknown
samples. Ensure that you read and understand the hazards of the chemicals used in the
following preparations. Dispose of all laboratory reagents by the appropriate method for a
specific reagent or solvent.
Material safety data sheets (MSDS) summarize information on the hazard and toxicity of
specific chemical compounds. They also describe the proper handling of compounds, first aid
for accidental exposure, and procedures for cleaning up spills or leaks. Producers and suppliers
of chemical compounds are required by law to provide their customers with the most current
health and safety information in the form of an MSDS. Read the material safety data sheets
for each chemical you use.
CAUTION Avoid exposure to potentially harmful materials. Always wear protective gloves
and safety glasses when you use solvents or corrosives. Also, contain waste streams and use
proper ventilation. Refer to your supplier’s material safety data sheets (MSDS) for
procedures that describe how to handle a particular solvent, corrosive substance, or both.
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Sample Formulations
Sensitivity Test Solutions
Sensitivity Test Solutions
The MSQ Sensitivity Test Kit (part number FM104284) contains two 25 mL glass vials. One
vial contains 10 mg of erythromycin, and the other vial contains 10 mg of p-nitrophenol. The
stock solutions, which can be stored under refrigeration for up to three months, are prepared
by adding 10 mL of solvent to each of these vials. The working solutions that are used to
perform the sensitivity test are prepared by diluting aliquots of the stock solutions.
Preparing the Stock Solutions for the Sensitivity Tests
The field service engineer uses two compounds to perform sensitivity testing. Erythromycin is
used to test the sensitivity of the instrument in the positive ion mode. P-nitrophenol is used to
test the sensitivity of the instrument in the negative ion mode.
Stock Solution: Erythromycin
Follow this procedure to perform sensitivity testing with erythromycin.
 To prepare a 1 mg/mL (1g/L) stock solution of erythromycin in 100% HPLC-grade
acetonitrile
1. Open the 25 mL glass vial that contains 10 mg of erythromycin.
2. Using a 10 mL volumetric pipette or an adjustable, mechanical pipette, add 10 mL of
HPLC-grade acetonitrile to the vial.
3. Screw the cap back onto the vial and mix.
4. Label the vial Erythromycin Stock Solution.
5. Store the erythromycin stock solution (1 mg/mL) in a refrigerator until it is needed.
When refrigerated, this stock solution is stable for several months.
Stock Solution: P-Nitrophenol
Follow this procedure to perform sensitivity testing with p-nitrophenol.
 To prepare a 1 mg/mL (1mg/mL) stock solution of p-nitrophenol in 100% HPLC-grade
methanol
1. Open the 25 mL glass vial that contains 10 mg of p-nitrophenol.
2. Using a 10 mL volumetric pipette or an adjustable, mechanical pipette, add 10 mL of
HPLC-grade methanol to the vial.
3. Screw the cap back onto the vial and mix.
4. Label the vial P-Nitrophenol Stock Solution.
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Sample Formulations
Sensitivity Test Solutions
5. Store the p-nitrophenol stock solution (1 mg/mL) in a refrigerator until it is needed.
When refrigerated, this stock solution is stable for several months.
Preparing the Working Solutions for the Sensitivity Tests
A working solution of 5 pg/μL erythromycin in 50:50 acetonitrile/water and a working
solution of 2 pg/μL p-nitrophenol in 50:50 methanol/water are required to perform the
sensitivity tests. Prepare these working solutions daily as needed from the refrigerated stock
solutions. The following procedures require the materials listed in Table 11 or their
equivalents.
Table 11. Required materials
Item
Quantity
200 L adjustable, mechanical pipette (calibrated) or equivalent
1
1 mL adjustable, mechanical pipette (calibrated) or equivalent
1
10 mL adjustable, mechanical pipette (calibrated) or equivalent
1
25 mL glass vials with caps or equivalent
6
50:50 acetonitrile/water (v/v), premixed
30 mL
50:50 methanol/water (v/v), premixed
30 mL
Note The following procedures result in a 200000-fold dilution of the erythromycin
stock solution and a 500000-fold dilution of the p-nitrophenol stock solution. Alternative
dilution procedures that result in a 5 pg/L solution of erythromycin and a 2 pg/L
solution of p-nitrophenol with a reasonable level of accuracy are acceptable.
Preparing the Erythromycin Sensitivity Test Solution
Use this procedure to prepare the erythromycin sensitivity test solution.
 To prepare 10 mL of the erythromycin sensitivity test solution
1. Allow the 1 mg/mL (1 μg/μL) stock solution of erythromycin to equilibrate to room
temperature. Then dilute it 100-fold:
a. Using a 200 μL adjustable pipette, transfer 100 μL of the stock solution of
erythromycin into a clean, dry 25 mL glass vial.
b. Using a 10 mL adjustable pipette, add 9.9 mL of 50:50 acetonitrile/water (v/v) to the
vial, and mix the solution.
c. Label the resulting solution 10 ng/μL Erythromycin.
2. Dilute the 10 ng/μL solution of erythromycin 100-fold:
a. Using a 200 mL adjustable pipette, transfer 100 mL of the 10 ng/mL solution of
erythromycin into a clean, dry 25 mL glass vial.
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Sample Formulations
Sensitivity Test Solutions
b. Using a 10 mL adjustable pipette, add 9.9 mL of 50:50 acetonitrile/water (v/v) to the
vial, and mix the solution.
c. Label the resulting solution 100 pg/μL Erythromycin.
3. Dilute the 100 pg/μL solution of erythromycin 20-fold:
a. Using a 1 mL adjustable pipette, transfer 0.50 mL of the 100 pg/mL erythromycin
solution into a clean, dry 25 mL glass vial.
b. Using a 10 mL adjustable pipette, add 9.5 mL of 50:50 acetonitrile/water (v/v) to the
vial, and mix the solution.
c. Label the resulting solution 5 pg/μL Erythromycin.
Preparing the P-Nitrophenol Sensitivity Test Solution
Use this procedure to prepare the p-nitrophenol sensitivity test solution.
 To prepare 10 mL of the p-nitrophenol sensitivity test solution
1. Allow the 1 mg/mL (1 μg/μL) stock solution of p-nitrophenol to equilibrate to room
temperature. Then, dilute it 100-fold:
a. Using a 200 μL adjustable pipette, transfer 100 μL of the stock solution of
p-nitrophenol into a clean, dry 25 mL glass vial.
b. Using a 10 mL adjustable pipette, add 9.9 mL of 50:50 methanol/water (v/v) to the
vial, and mix the solution.
c. Label the resulting solution 10 ng/μL P-nitrophenol.
2. Dilute the 10 ng/μL solution of p-nitrophenol 100-fold:
a. Using a 200 μL adjustable pipette, transfer 100 μL of the 10 ng/μL solution of
erythromycin into a clean, dry 25 mL glass vial.
b. Using a 10 mL adjustable pipette, add 9.9 mL of 50:50 methanol/water (v/v) to the
vial, and mix the solution.
c. Label the resulting solution 100 pg/μL P-nitrophenol.
3. Dilute the 100 pg/μL solution of p-nitrophenol 50-fold:
a. Using a 200 mL adjustable pipette, transfer 200 μL of the 100 pg/μL p-nitrophenol
solution into a clean, dry 25 mL glass vial.
b. Using a 10 mL adjustable pipette, add 9.8 mL of 50:50 methanol/water (v/v) to the
vial, and mix the solution.
c. Label the resulting solution 2 pg/μL P-nitrophenol.
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A Sample Formulations
Preparing the MSQ Plus Mass Detector Calibration Solution
Preparing the MSQ Plus Mass Detector Calibration Solution
This section describes how to prepare the MSQ Plus Mass Detector calibration solution for autotune
and mass calibration. You can also find these instructions in the MSQ Plus Mass Detector Calmix Kit
Preparation Guide.
Kit Contents
The MSQ Plus Mass Detector Calmix Kit (Part Number 60111-62021) includes a
concentrated stock calibration solution of three inorganic salts, with the following
concentrations:
• Sodium iodide (NaI): 130 mM
• Potassium iodide (KI): 5 mM
• Cesium iodide (CsI): 2 mM
The inorganic salts are dissolved in 2.5 mL (± 1%) of solvent and packaged in a sealed
ampoule. The solvent is a 92:8 mixture of HPLC-grade water (H2O) and reagent-grade
isopropanol (IPA). The neck of the ampoule is scored for easy and safe opening.
Three ampoules and three labels are included in the Calmix Kit. The labels denote the
expiration date (36 months from date of manufacture), the lot number, and the Thermo
Fisher Scientific and supplier-provided part numbers. The Calmix Kit contains a gravimetric
certificate of analysis corresponding to the lot number. It also includes the Material Safety
Data Sheet (MSDS) for the calibration solution.
Preparing the Calibration Solution
Follow these steps to prepare the calibration solution.
 To prepare the working concentration of calibration solution
1. In a graduated cylinder, prepare 250 mL (± 20 mL) of diluent:
• 125 mL (± 10 mL) of HPLC-grade H2O
• 125 mL (± 10 mL) of reagent-grade IPA
Note The efficacy of the calibration solution depends on high-quality diluent
and clean glassware, because the solvent adducts must be properly formed in the
electrospray source and the reference masses must be detected against the
background of electronic and chemical noise.
2. Transfer 100 mL (± 10 mL) of the diluent from the graduated cylinder to the 250 mL
Nalgene bottle that is attached to the front panel (inlet side) of the MSQ Plus Mass
Detector.
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Sample Formulations
Calibrant Solution
3. Transfer the entire contents of a single ampoule to the Nalgene bottle. You can use a
transfer pipet to facilitate the transfer.
4. Transfer the remaining volume of the diluent from the graduated cylinder to the Nalgene
bottle.
5. Attach one of the labels from the Calmix Kit to the Nalgene bottle.
6. Firmly seal the Nalgene bottle of the MSQ Plus Mass Detector inlet reservoir and mix the
solution by swirling the bottle.
Note The Nalgene bottle is pressurized from 1.0 to 1.5 bar during the autotune and
mass calibration process, requiring a leak-tight seal.
7. Once the autotune and mass calibration process is complete, bypass the injection valve by
attaching the transfer line from the HPLC system to the grounded union on the front
panel of the mass detector. This last step does not apply to MSQ systems that are
grounded through the injection valve—that is, released before January 2005 (serial
number below 201 xx).
Calibrant Solution
The automated full-system autotune and mass-scale calibration procedures use a calibrant
solution that contains a mixture of 1.3 mM sodium iodide (NaI), 0.05 mM potassium iodide
(KI), and 0.02 mM cesium iodide (CsI) in a solution of 50:50 isopropanol/water. You can
purchase this calibrant solution as a new kit (part number 60111-62021) containing Calmix
concentrate and instructions. This material ships with each new MSQ Plus Mass Detector as
part of the MSQ chemical kit (part number 60111-62023). Or, you can prepare the solution
yourself, using the procedures in this section.
Preparing the calibrant solution requires the materials and equipment listed in Table 12 or
their equivalents.
To prepare 250 mL of calibrant solution, follow these procedures:
1. Preparing a Concentrated Stock Solution of the Calibration Mixture
2. Preparing the Working Calibration Solution
Table 12. Materials and equipment required to prepare calibrant (Sheet 1 of 2)
106
Materials and equipment
Quantity
Cesium iodide (CsI), > 99.9% pure
Approx. 300 mg
Potassium iodide (KI), A.C.S. reagent grade
Approx. 500 mg
Sodium iodide (NaI), A.C.S. reagent grade
Approx. 10 g
HPLC-grade water
Approx. 200 mL
Isopropanol
Approx. 200 mL
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Sample Formulations
Calibrant Solution
Table 12. Materials and equipment required to prepare calibrant (Sheet 2 of 2)
Materials and equipment
Quantity
125 mL Erlenmeyer flask (or equivalent)
1
250 mL plastic graduated cylinder
1
1 mL adjustable, mechanical pipette (calibrated) or equivalent
1
Analytical balance
1
Preparing a Concentrated Stock Solution of the Calibration Mixture
The stock solution contains 10 mM cesium iodide (MW 259.8 g/mole), 25 mM potassium
iodide (MW 166.0 g/mole), and 650 mM sodium iodide (MW 149.9 g/mole) in 50:50
isopropanol/water.
 To prepare a stock solution of the calibration mixture
1. Add 50 mL of HPLC-grade water to the 125 mL Erlenmeyer flask.
2. Weigh out 260 mg ± 10 mg of cesium iodide. Then, quantitatively transfer the cesium
iodide to the flask. Record the actual weight, manufacturer, and lot number of the cesium
iodide (CsI) used.
3. Weigh out 415 mg ± 10 mg of potassium iodide. Then, quantitatively transfer the
potassium iodide to the flask. Record the actual weight, manufacturer, and lot number of
the potassium iodide (KI) used.
4. Weigh out 9.74 g ± 0.10 g of sodium iodide. Then, quantitatively transfer the sodium
iodide to the flask. Record the actual weight, manufacturer, and lot number of the
sodium iodide (NaI) used.
5. Add 50 mL of isopropanol to the flask and swirl to mix the solution.
6. Allow the solution to cool, and transfer the solution to a 100 mL glass bottle.
7. Label the bottle 10 mM CsI, 25 mM KI, and 650 mM NaI in 50:50 IPA/H2O. In
addition, label the bottles, as specified in step 2, step 3, and step 4.
Preparing the Working Calibration Solution
The working calibration solution used to calibrate the MSQ Plus Mass Detector can be
prepared by diluting the stock solution 500-fold with a pre-mixed solution of 50:50
isopropanol/water (v/v).
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Sample Formulations
Calibrant Solution
 To prepare the working calibration solution
1. Prepare approximately 250 mL of the 50:50 isopropanol/water (v/v) diluent.
2. Unscrew the cap of the reference reservoir bottle, and remove the bottle from the MSQ
Plus Mass Detector.
3. Dispose of any leftover calibrant solution from the reference reservoir bottle, and rinse the
reference reservoir bottle with 50:50 isopropanol/water to remove any remaining residue.
4. Add approximately 100 mL of the premixed 50:50 isopropanol/water (v/v) to a 250 mL
plastic graduated cylinder.
5. Using a 1 mL adjustable, mechanical pipette (calibrated) or equivalent, add 0.5 mL of the
stock solution [10 mM CsI, 25 mM KI, and 650 mM NaI in 50:50 IPA/H2O (v/v)] to
the graduated cylinder.
6. Fill the graduated cylinder to the 250 mL mark with premixed
50:50 isopropanol/water (v/v).
The working calibration solution contains 1.3 mM NaI, 0.05 mM KI, and 0.02 mM CsI
in 50:50 isopropanol/water.
7. Transfer the calibrant solution to the clean reference reservoir bottle. Then reinstall the
reservoir bottle into your MSQ Plus Mass Detector. Ensure that the bottle cap is tightly
closed. See Figure 75.
Figure 75. View of refilled reference inlet reservoir
Nitrogen
pressurization line
BOTTLE CAP
MUST BE
CLOSED
TIGHTLY
108
Clip
Clip
Waste
bottle
Reference inlet reservoir
containing calibrant
MSQ Plus Mass Detector Getting Started Guide
Thermo Scientific
I
Index
A
C
Accela LC system 2, 5, 21
acetic acid 6
active but unpinned cells 90
adduct ions 4
ammonium acetate 6
ammonium formate 6
ammonium hydroxide 6
analytical LC 6
anti-virus software 16
APCI probes 2, 50
APCI. See atmospheric pressure chemical ionization
apcineg.tune file 9
apcipos.tune file 9, 54
atmospheric pressure chemical ionization 2
effect of acidity and basicity 5
flow rates 5
non-polar compounds 3, 5
parameters to optimize 8
polarity modes 5
probe temperature 6
tuning
acquiring data in raw file 54
opening Instrument Setup view 51
optimizing acquisition parameters 49
selecting tune options 51
autosamplers
function 1, 8
introducing samples 5
specifying injection volume and vial location 46
autotuning 1
calibrant solution
contents of 25
preparing 101, 105–107
viewing mass spectrum of 30
capillary LC 6
Change Instruments In Use dialog box 83
chromatogram cells 86
Chromatogram Ranges dialog box 86–87
Chromatogram view 58, 60–61, 70
Column Arrangement dialog box 78
Column Arrangement icon 78
compliance
FCC iii
regulatory iii
compliance, WEEE v
cone voltage 40, 46, 50, 55
Confirm Set Electron Multiplier Voltage dialog box 37
contacting us xiv
corona current 6, 8–9, 50, 55, 68
corona pin 49
B
borate 6
Thermo Scientific
D
deep tune 7–8
detector gain calibration 23, 34–35
Direct Control dialog box 25, 40, 51, 55
documentation survey xv
E
Edwards forepumps 19
electromagnetic compatibility iii
electron multiplier gain 35
electrospray
effect of buffers on sensitivity 4
effect of solvent pH 5
factors affecting 4
molecular weights of compounds 4
MSQ Plus Mass Detector Getting Started Guide
109
Index: F
parameters to optimize 8
performed by MSQ Plus Mass Detector 2
polar compounds 3–4
polarity modes 4
probe temperature 6
recommendations for generating stable 4
setting up for calibration 24
spectra produced 4
tuning
acquiring data in raw file 44
optimizing acquisition parameters 39
erythromycin 102
erythromycin stock solution 102
ESI probes 2, 25, 50
ESI. See electrospray
esineg.tune file 9
esipos.tune file 9, 40, 43
F
FCC compliance iii
File Save - Audit Trail dialog box 72
File Summary Information dialog box 71, 79
flow rates 5
fluorescence detectors 1
forepumps
checking oil level in 18
draining oil from 20
Edwards 19
function 2
Full Scan Events table
opening 58
parameters on 68
purpose 62
switching to SIM Scan Events table 60
viewing mass spectrum of calibrant solution 30
full-system autotune
performing 31
purpose 7
report 33
when to perform 8, 23
G
gain calibration 35
H
hard drives 16
Hide Method Options bar 60
high-mass resolution 9
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MSQ Plus Mass Detector Getting Started Guide
I
inactive cells 90
Info View icon 18
Information view
displaying 18
purpose 17
Instrument Method report 95
instrument methods 65
Instrument Setup view 40, 51, 65
Instrument Tuning and Calibration wizard 32, 34–35
ion energy 9, 40, 51, 54
ion source 2, 6
ionization mode 68
L
LC pumps
acquiring data in Tune window 41, 51
draining oil from oil mist filter 20
increasing flow rate and temperature 7
introducing samples to MSQ Plus Mass Detector 5
minimum backpressure required 8
optimizing mass detector parameters 8
performing detector gain calibration 23
performing full-system autotune 23, 25, 32
performing mass-scale calibration 23, 25, 34
setting up 25
stopping after end of sequence 84
viewing calibrant mass spectrum 31
LC system
checking hardware 15
configuring 13
connecting 15
creating instrument method controlling 70
opening Instrument Setup window 51
preparing for operation 21
setting up for calibration 25
setting up for manual tuning 40
light-scattering detectors 1
liquid chromatography mass spectrometry (LC/MS) 1
Locked icon 85
low-mass resolution 9
M
mass analyzer 2
mass-scale calibration
performing 34
purpose 8
reference inlet system used in 1
when to perform 8, 23, 34
material safety data sheets (MSDS) 101
Thermo Scientific
Index: N
.meth file 71
Method Editor
Chromatogram view 61
components of 58
Full Scan Events table 62
Method Options view 60
Per Method Parameters table 62
Preview menu 64
Scan menu 64
selecting method options 67
SIM Scan Events table 63
Welcome page 66
Method Options view 58, 60, 67
microbore LC 6
MSQ Plus button 66
MSQ Plus Configuration dialog box 11–12
MSQ Plus Mass Detector
communication with Xcalibur data system 17
components of 2
configuring 11
default tune parameters 8
features of 1–2
interface to Accela LC system 2, 5
levels of calibration in 23
Method Editor. See Method Editor 58
preparing for calibration 24
purpose of 1
serial number of 13
setting up for ESI mode 24
states of 16
types of ionization performed 2–3
Welcome page 57
MSQ Plus Mass Detector Calmix Kit 105
MSQ Plus Method Editor dialog box 10
MSQ Sensitivity Test Kit 102
N
needle voltage 6, 8–9, 39–40, 46, 68, 97
New Sequence Template dialog box 75–76
nitrogen gas 6, 15–16, 26
nitrogen regulators 15
non-polar compounds 3
non-volatile buffers 6
O
oil mist filters
contamination of 18
draining oil from 20
Open dialog box 81
Open Raw File dialog box 45, 55, 93
Thermo Scientific
P
Per Method Parameters table
displaying 27, 44, 62
purpose 62
phosphate 6
pin icon 85, 89
p-nitrophenol 102
p-nitrophenol sensitivity test solution 104
p-nitrophenol stock solution 102
polar compounds 3–4
polarity switching 62
Preview menu 64
probe heaters 26–27
probe temperature
APCI 6, 50, 55
ESI 6, 40, 46
resetting 28
setting 62, 68
Q
Qual Browser
active but unpinned cells 90
adding cells to the grid 94
inactive cells 90
opening raw files 92
purpose 89
viewing mass spectrum for a time point 98
viewing reports 95
Qual Browser icon 45, 92
Qual Browser window 45, 55
R
raw files
acquiring data in 44, 54
creating file name for 41, 51
opening in Qual Browser 89
opening in Qual browser 92
opening previously acquired 61
storing 77
Real Time Plot View icon 85
reference inlet reservoirs 26, 108
reference inlet system 5
checking solvent levels 25
flushing air out of 28
use in autotuning 1
use in mass-scale calibration 1
refractive index detectors 1
regulatory compliance iii
resolution set-up 7
RF lens bias 8–9, 40, 51, 54
MSQ Plus Mass Detector Getting Started Guide
111
Index: S
Roadmap view 18
Run Sample icon 82
Run Sequence dialog box 82
S
safety precautions xiii
safety standards iii
Save As dialog box 71, 79
Save Tune Parameters dialog box 47, 56
scan events 69
Scan menu 64
Select Tune Parameter File Name dialog box 43, 53
Select Tune Raw File Name dialog box 42, 53
sensitivity test solutions
erythromycin 103
p-nitrophenol 104
preparing 101
sequence file 81
Sequence Setup icon 75
Sequence Setup view 75, 77
sequences 75
serial number of the mass detector 13
Server icon
appearance on starting Xcalibur data system 18
states of 17
status of MSQ Plus Mass Detector 17
Server shortcut menu 27
server software 17
Show Full Scans bar 60
Show Method Options bar 60, 67
Show Per Method Parameters Table bar 62
Show SIM Scans bar 60
SIM scan events 63
SIM Scan Events table
opening 58
parameters on 68
purpose 63
switching to Full Scan Events table 60
.sld files 81
source fragmentation 62
spectrum view 61
Start Acquiring icon 45, 55
Status Log report 96
Stop Acquiring icon 45, 47, 55
survey link xv
112
MSQ Plus Mass Detector Getting Started Guide
T
Thermo Foundation Instrument Configuration
dialog box 11
triethylamine (TEA) 6
trifluoroacetic acid (TFA) 6
tune files
saving optimized settings in 8
selecting in Method Editor 68
Tune Method report 97
Tune Options dialog box 29, 41, 52
tune report locations 37
tuning calibrant 26
tuning in APCI mode. See atmospheric pressure chemical
ionization
tuning in ESI mode. See electrospray
turbomolecular pumps 2
U
Unlocked icon 85
USB cables 18
UV detectors 1
V
vacuum system 2
View bar 66
volatile buffers 6
W
waste bottle 26
WEEE compliance v
Welcome page 57, 66
X
Xcalibur data system
anti-virus software 16
communication with MSQ Plus Mass Detector 17
icon 18
Information view 17
Instrument Setup view 40, 51, 65
parameters imported from tune file 8
Roadmap view 18
Sequence Setup view 75, 77
server software 17
starting 18
Thermo Scientific
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