Analytik Jena BioShake iQ, XP Shaker and thermal mixer User manual
Below you will find brief information for Shaker and thermal mixer BioShake iQ, Shaker and thermal mixer BioShake XP. This document describes the functionality of the two models of the BioShake Series, the BioShake iQ and the BioShake XP. These devices are designed for shaking and temperature control of liquids in tissue culture plates, microplates, tubes and glass vials. It is possible to control mixing speeds from 200 rpm up to 3,000 rpm. Temperature can be controlled from room temperature up to 99 °C. The unit allows the user to define two automatic programs, each with three different steps, allowing for flexible and precise control of shaking and temperature parameters.
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BioShake Series
Shaker and thermal mixer
Analytik Jena
Lifescience
Konrad-Zuse-Str. 1
07745 Jena
Phone: e-mail:
Hotline:
Fax:
+ 49 (3641) 77-94 00
+ 49 (3641) 77-76 77 76 [email protected]
General information about Analytik Jena AG on the internet: http://www.bio.analytik-jena.de
Edition – June 2013
Technical documentation made by:
Analytik Jena
This documentation describes the state of this product at the time of publishing. It need not necessarily agree with future versions of the product.
Subject to change!
© Copyright 2013 Analytik Jena
Content
3
3.1
3.2
4
5
6
6.5
6.6
6.7
6.8
6.1
6.2
6.3
6.4
6.9
6.10
6.11
7
8
8.1
8.2
9
10
10.1
10.2
10.3
10.4
Content
2
2.1
2.2
2.3
1
1.1
1.2
Introduction ............................................................................. 3
Field of application ........................................................................................... 3
Information on the use of this manual .............................................................. 4
Safety instructions .................................................................. 5
Warning and safety symbols on the BioShake ................................................. 5
Safety and caution information ......................................................................... 5
General information .......................................................................................... 7
Delivery parts .......................................................................... 8
BioShake iQ ..................................................................................................... 8
BioShake XP .................................................................................................... 8
Installation ............................................................................... 9
Changing of adapter plates .................................................. 10
Operation ............................................................................... 11
Control panel of BioShake XP ........................................................................ 11
Control panel of BioShake iQ ......................................................................... 12
Standard functions ......................................................................................... 12
Set up time ..................................................................................................... 13
Set up mixing speed ....................................................................................... 13
Set up temperature......................................................................................... 15
Short mix ........................................................................................................ 15
Start / Stop ..................................................................................................... 15
Programming .................................................................................................. 15
Set up the time counting and temperature control mode ................................ 16
Set up the buzzer and programming control mode ........................................ 17
Tips for shaker operation ..................................................... 18
Specification ......................................................................... 19
Adapter for microplates, tubes and glass vials ............................................... 19
Technical specifications ................................................................................. 19
Ordering information ............................................................ 21
Maintenance and repair ........................................................ 24
Cleaning and maintenance ............................................................................. 24
Servicing ........................................................................................................ 24
Replacement of spare parts ........................................................................... 24
Other accessories .......................................................................................... 24
User manual BioShake series Issue 06/2013 1
Content
11
11.1
12
13
14
15
16
Service ................................................................................... 25
Instructions for return shipment ...................................................................... 25
Equipment Decontamination Certificate ............................. 26
Note for disposal of electric/electronic waste .................... 27
EC - Declaration of Conformity ............................................ 28
Warranty ................................................................................ 29
Table of figures ..................................................................... 31
2 Issue 06/2013 User manual BioShake series
Introduction
1 Introduction
1.1 Field of application
The BioShake Series is special designed for precise control of speed and temperature in an apparatus with a footprint not bigger than 2 microplates side-by-side.
The apparatus offer an ultra-efficient, 3-dimensional shaking axis to mix samples in tubes, glass vials or microplates (up to 1536 wells) without the need of spinning down the samples after mixing. Even high density plates or low samples volumes are not a problem for the Shaker.
BioShake mixers have an orbit diameter of 2.0 mm and a high maximum shaking frequency of 3,000 rpm, allowing any model to easily mix standard and low volume samples in tubes, glass vials and 96-well, 384-well or 1536-well microplates.
The apparatus are working very quiet due to a special patent pending dampening system which relies upon ingenious counter-weight symmetry. They shake tubes to mix their content but do not put vibrations on the entire work bench or robotic deck.
Reduced mass sample blocks provide for quick heating and accurate temperature control across the whole block.
Fig. 1-1 Correlation between mixing frequencies and mixing effects in dependence on the filling level
User manual BioShake series Issue 06/2013 3
Information
1.2 Information on the use of this manual
The following warning and information symbols are used in this manual:
Danger! Dangerous touch voltage!
Caution!
Warning messages alert you to a specific procedure or practice which, if not followed correctly, could cause personal injury.
Caution! Hot surface!
Contact with the surface can cause burns.
Attention!
Caution messages refer you to procedures which, if not observed, could result in damage to the equipment.
Disconnect mains plug!
Note
This information must be observed to ensure correct function of the device.
The following systematic approach is introduced in the manual:
•
The chapters and figures are numbered consecutively.
•
Each image has its own caption.
•
A cross reference is indicated with an arrow (e.g. → Information on the use of this manual pg. 4).
•
Working steps are numbered.
4 Issue 06/2013 User manual BioShake series
Safety instructions
2 Safety instructions
Read through this chapter carefully prior to commissioning the BioShake Series for your own safety and for trouble-free operation.
Follow all the safety instructions explained in the manual, as well as all messages and information displayed on the screen by the internal microcomputer.
2.1 Warning and safety symbols on the BioShake
The following warning and safety symbols are affixed on the BioShake:
Caution! Hot surface!
Contact with the surface can cause burns!
This symbol is affixed on the basis unit next to the heating surface of the block (only
BioShake iQ).
Disconnect mains plug!
This symbol is affixed on the rear of the device next to the mains input.
2.2 Safety and caution information
1. Do not operate the device in rooms where work is being carried out with explosive substances.
2.
Do not use this device to process any explosive, radioactive or highly reactive substances.
3.
Do not use this device to process any substances, which could create an explosive atmosphere.
4.
Danger! Electric shock from damage to device/power cable. Danger! Lethal voltages inside the device.
5.
Warning! General hazard point.
6.
Warning! Injury from rapidly rotating holder. Injury from rapidly rotating imbalance compensation. Injury from flying tubes and plates.
7.
Warning! Injury from sample material being flung out. Injury from incorrect vortexing.
8.
Warning! Damage due to incorrect power supply.
User manual BioShake series Issue 06/2013 5
Safety instructions
9. Caution! Damage to the display from mechanical pressure. Severe vibration.
10.
Caution! Damage to electronic components caused by spilled liquids. Damage to electronic components from condensation.
11.
Caution! Poor safety due to missing operating manual. Caution when using aggressive chemicals.
12.
Please start even with minimal mixing frequencies to avoid overloading.
13. Only mix in sealed tubes and plates. Sample material can be flung out of open, inadequately sealed or unstable tubes and plates.
14. When working with hazardous, toxic and pathogenic samples, always comply with the nationally specified safety environment.
15.
Please use the original power supply delivered by Analytik Jena | Biometra.
16.
Please use the original accessories recommended by Analytik Jena | Biometra.
Pay particular attention to personal safety gear (gloves, clothing, glasses etc.), the extraction hood and the safety class of the laboratory.
Read this section carefully and follow the instructions before beginning installation and any procedure.
The unit must be placed on a horizontal solid work space.
The device must be saving from shocks and falling.
The unit must be placed in sufficient distance to heat registers or radiators to ensure ambient temperature conditions in accordance with the technical specifications (section 8.2).
Use the normal care and precaution one would use with any electrical appliance.
Be sure to use a power cord with the same rating and of the same type (three-wire with neutral conductor) as the one supplied with the instrument.
Ensure that voltage and frequency of your power source match the input voltage and frequency inscribed on the electrical rating label of the power supply (AC/DC adaptor) and that the output voltage matches the rating of the equipment. The AC/DC adaptor is designed to work with a single-phase power system having a grounded neutral conductor.
If you are not sure what type of power is supplied to your building contact your facilities manager.
As BioShake is an electrical device do not spill large quantities of liquid onto the device. Do not use it in an explosive atmosphere.
As parts of the device may generate electric, magnetic or electromagnetic fields, keep parts away that may be affected (e.g. data storage units).
6 Issue 06/2013 User manual BioShake series
Safety instructions
BioShake is capable of heating the plate surface up to 100 °C.
This temperature is high enough to cause serious burns if touched.
Use extreme caution at all times.
Never leave your unit accessible to others when it is hot.
Never touch the plate surface unless you are sure it is cold.
Use oven gloves if necessary.
Do not unscrew any part of BioShake except for the interchangeable power supply, the power cord and different blocks.
Do not make any mechanical or electrical modifications to the equipment.
Never do anything else with BioShake as intended in this manual.
Noncompliance of the safety instructions may lead to device damage, loss of warranty and may cause serious personal injury as well as death.
2.3 General information
Warning! Injury from sample material being flung out.
Sample material can be flung out of open, inadequately sealed or unstable wells, plates and tubes.
•
Only mix in sealed tubes and plates
•
When working with hazardous, toxic and pathogenic samples, always comply with nationally specified safety environment. Pay particular attention to personal safety gear (gloves, clothing, glasses etc.), the extraction hood and the safety class of the laboratory.
When setting mixing frequency, adhere to the rules outlined in
Fig. 6-4 to 6-7.
User manual BioShake series Issue 06/2013 7
Delivery parts
3 Delivery parts
3.1 BioShake iQ
(3)
1 BioShake iQ
2 Power supply
3 Power cord
Operating manual (no picture)
(1)
(1)
(2)
(1)
Fig. 3-1 BioShake iQ (1) with power supply (2) and power cord (3)
3.2 BioShake XP
(3)
1 BioShake iQ
2 Power supply
3 Power cord
Operating manual (no picture)
(1)
(2)
Fig. 3-2 BioShake XP (1) with power supply (2) and power cord (3)
8 Issue 06/2013 User manual BioShake series
Installation
4 Installation
Unpack and carefully check the instrument. Report any damage or missing items to your local distributor.
If no damage is found place the device up on a stable horizontal surface.
Fig. 4-1 Connections at the rear side of device (BioShake iQ and BioShake XP)
Before starting to work with the BioShake:
Remove the cover plate before installing an adapter plate:
Loose the 3 torx screws with the torx screwer at the adapter packaging and remove the cover plate.)
If the adapter will not be removed before installing an adapter, the adapter will not be recognized by the device!
Place an adapter on the shaker (chapter 5: Changing of adapter plates).
Plug the external power supply (2) into the 24 V socket at the rear side of the BioShake iQ or BioShake XP.
Plug the power cable (3) into the power supply (2) and into the wall socket.
Turn On the instrument.
It is advisable to carry out a test run at maximum speed to ensure that the device does not move while mixing.
User manual BioShake series Issue 06/2013 9
Changing of adapter plates
5 Changing of adapter plates
To change the adapter plates, please follow the subsequent steps:
Attention!
If the security cover plate or an adapter plate already be mounted, please remove it first!
1. Turn off the instrument!
2. Remove all sample carriers B-3 (tubes, vials, microplates etc.).
3. Loosen the 3 torx screws A-1, A-2 and A-3 by using the supplied screwdriver
(Torx size 8, rotate left).
4. Take off the cover clate or adapter plate B-2 straight up and put it on a clean, soft surface.
Mounting of the new adapter plate:
1. Please take care to a clean, dirt-free and particle-free operation.
2. Insert the new adapter plate straight into the impression of the plate holder B-1.
3. Check the fixed position.
4. Fix all torx screws A-1, A-2 und A-3 using clockwise rotation. Please take care to uniform controlled tightening of screws to ensure a good fit.
5. Please take care to good and tight fit of sample carriers B-3 (tubes, vials, microplates etc.).
6. Turn on the instrument! BioShake will recognize the different block types. All pending information and indications are shown in the display.
B-3
B-2 A-2
A-3
A-1
10
B-1
Fig. 5-1 Mounting of adapter plates
Attention!
Heavier blocks may limit the shaking speed (see Fig. 6-6 Recommended maximum mixing speeds)
Issue 06/2013 User manual BioShake series
Operation
6 Operation
The BioShake is designed for shaking (BioShake iQ and BioShake XP) and temperature control (BioShake iQ) of liquids in tissue culture plates, microplates, tubes and glass vials. Microplates or tubes have to be placed on the shaker platform of the BioShake.
The liquid to be shaken is introduced into the wells or tubes.
The automatic start up function of the unit ensures a gradual start of the shaking process without sample splashing.
The drive is absolutely wear-free and maintenance-free.
6.1 Control panel of BioShake XP
The following picture shows the control panel of the BioShake XP.
Fig. 6-1 Control panel of BioShake XP
User manual BioShake series Issue 06/2013 11
Operation
6.2 Control panel of BioShake iQ
Fig. 6-2 Control panel of BioShake iQ
6.3 Standard functions
12
Fig. 6-3 Standard functions (only for BioShake iQ and BioShake XP)
Issue 06/2013 User manual BioShake series
Operation
6.4 Set up time
Use time cursors to set the required operation time.
If the timer is not selected, pressing the Start / Stop will cause a continuous operation of the shaker.
6.5 Set up mixing speed
It is possible to control mixing speeds from 200 rpm up to 3,000 rpm.
To set the mixing speeds use the mix buttons.
Required mixing speeds [rpm] for different tubes depending on
filling volume [%] for diluted fluids
Filling volume 0.2 ml tubes 0.5 ml tubes
25 % 1400 - 1800 1200 - 1600
1.5 ml tubes
1000 - 1300
2.0 ml tubes
1000 - 1300
50 %
75 %
1200 - 1500
1000 - 1300
1100 - 1300
1000 - 1200
1000 - 1200
900 - 1100
900 - 1200
900 - 1100
Fig. 6-4 Required mixing speeds depending on filling volume of tubes
Filling volume
10 %
Required mixing speeds [rpm] for microplates depending on filling
volume/well [%] for diluted fluids
96-well
(standard)
1800 - 2200
384-well
(standard)
2200 - 2600
384-well
(small-vol.)
2800 - 3000
1536-well
(standard)
2800 - 3000
25 %
50 %
75 %
1600 - 2000
1400 - 1800
1200 - 1600
2000 - 2400
1800 - 2200
1600 - 2000
2400 - 3000
2200 - 2600
2000 - 2400
2600 - 3000
2400 - 2600
2200 - 2600
Fig. 6-5 Required mixing speeds depending on filling volume of microplates
User manual BioShake series Issue 06/2013 13
Operation
14
Type
Microplate
Deep well plate
Deep well plate
Deep well plate
Maximal mixing speeds [rpm] for microplates depending on the
weight [g]
Weight
0 -80 g
0 -120 g
0 -150 g
150 -300 g
1,400
x
2,000
x
2,500
x
3,000
x
Fig. 6-6 Recommended maximum mixing speeds
Adapter categories:
All adapters will be automatically detected while mounting on the instrument. The allocation of the adapter to a particular category determines the properties.
Adapter category
Max. rpm
Cat 01
Cat 02
Cat 03
Cat 04
Cat 05
Cat 06
Cat 07
Cat 10
3,000
1,800
2,200
3,000
1,000
----
2,500
OEM
Available adapters
Item no. Description
-
848-1808-1061
848-1808-1062
A) Without adapter (heating function not available)
B) All non classified adapters (heating function not available)
Adapter for 24x 2.0 ml or 15x 0.5 ml tubes
Adapter for 24x 1.5 ml or 15x 0.5 ml tubes
848-1808-1063
848-1808-1065
848-1808-1066
848-1808-1067
848-1808-1071
848-1808-1072
848-1808-1121
848-1808-1131
848-1808-1041
848-1808-1064
Adapter for 40x 0.5 ml or 28x 0.2 ml tubes
Adapter for 35x 2.0 ml or 24x 0.5 ml tubes
Adapter for 35x 1.5 ml or 24x 0.5 tubes
Adapter for 35x Lysis tubes 0.5 – 2.0 ml
Adapter for 30x 2.0 ml glass vials
Adapter for 20x 4.0 ml glass vials
Deep well plate adapter – 96 well / 1,000 µl (Eppendorf)
Deep well plate adapter - 96 well / 500 µl (Eppendorf)
Microplate adapter - 96 well standard PCR plate, universal
Adapter for 96x 0.2 ml tubes
Microplate adapter - flat bottom 848-1808-1021
848-1808-1031
848-1808-1093
Microplate adapter (universal) - 96 well round bottom
Adapter-Falcon tubes – 4 x 50 ml or 2 x 15 ml
848-1808-1094 Adapter-Falcon-tubes – 12 x 15 ml
Not available at the time
848-1808-1022 Microplate adapter - flat bottom, high base
848-1808-1051
Customer specified
Microplate adapter - 384 well standard PCR plates
Fig. 6-7 Mixing speed classification of adapters
Issue 06/2013 User manual BioShake series
Operation
All pending information and indications are shown in the display.
Display Line 1
Adapter Cat.: 01
Display Line 2
Max. Speed: 3000
6.6 Set up temperature
It is possible to control temperature from room temperature up to 99 °C.
To set the temperature use the Temp Buttons.
Attention!
Microplates melt at extremely high temperatures.
Only heat microplates made of polystyrene to a maximum of 70 °C
6.7 Short mix
By pressing Short Mix, the shaker will start up with the set parameters. Releasing the Short Mix button will stop the shaker.
6.8 Start / Stop
By pressing Start / Stop, the shaker will start up with the set parameters.
If you press Start / Stop again, the shaker will stop.
6.9 Programming
With the BioShake iQ and BioShake XP it is possible to have 2 automatic programs.
Both programs can have 3 different steps with different time, speed and temperature settings for each step.
User manual BioShake series Issue 06/2013 15
Operation
Program the BioShake iQ or BioShake XP with the following steps:
16
Programming BioShake iQ or BioShake XP Fig. 6-8
6.10 Set up the time counting and temperature control mode
2.
The time counting function enables temperature control and/or mixing for a limited period of time. There are two ways to measure the time of the temperature control processes.
Time control:
Temp control:
Time counting begins immediately when “Start/Stop” is pressed.
The Temp control mode is the standard mode. Time counting only starts when the nominal temperature value has been reached.
To terminate the process earlier, press “Start/Stop” again.
Switch the TCMo mode with the following steps:
1.
+
To start press Temp(+) and P1 and hold for at least
3 seconds.
Display Line 1
Display Line 2
TCMo BMo -
Temp auto
By pressing Time(+) or Time(-) the shaker will switch between
Temp mode or Time mode.
3.
+
To finish press P1 and Temp(+) and hold for at least 3 seconds.
Issue 06/2013 User manual BioShake series
Operation
6.11 Set up the buzzer and programming control mode
The programming control mode enables buzzer and temperature control at the end of automatic programs. There are two ways to set up the buzzer and temperature control process.
auto mode:
User mode:
At the end of automatic programs:
At the end of automatic programs:
•
•
3x short buzzer signals
Mixing is stopped
•
Temperature control is stopped and switch off
•
Short buzzer signal every 10 seconds
•
Mixing is stopped
•
Temperature control is enabled and keep target temperature constant
Switch the BMo mode with the following steps:
1.
+
To start press Temp(+) and P1 and hold for at least
3 seconds.
2.
Display Line 1
TCMo BMo -
Display Line 2
Temp auto
By pressing Mix(+) or Mix(-) the shaker will switch between
auto mode or user mode.
3.
+
To finish press P1 and Temp(+) and hold for at least 3 seconds.
User manual BioShake series Issue 06/2013 17
Tips for shaker operation
7 Tips for shaker operation
A wide variety of well plates are commercially available.
To ensure that the plates are positioned securely in the plate holder, they must correspond with the ANIS/SBS Standard for
Microplates.
If the filled plate has a weight of more than 80 g, then the maximum rated shaking frequency of 3,000 rpm may not be attainable with a safety stand. Select a lower shaking frequency in this case. However, there is no risk of damage to the BioShake – even if the weight is too high then the shaking action stops due to excessive weight or excessive shaking frequencies. As soon as you reduce the frequency, the unit will stand firm.
Maximum shaker platform load: 500 g (filled well plate).
The shaker is driven by a maintenance-free brushless motor which enables silent operation and constant shaking speed independent of the load.
IMPORTANT NOTES:
• Please start even with minimal mixing frequencies to avoid overloading.
• Only mix in sealed tubes and plates. Sample material can be flung out of open, inadequately sealed or unstable tubes and plates.
• When working with hazardous, toxic and pathogenic samples, always comply with the nationally specified safety environment.
• Pay particular attention to personal safety gear (gloves, clothing, glasses etc., the extraction hood and the safety class of the laboratory.
18 Issue 06/2013 User manual BioShake series
Specification
8 Specification
8.1 Adapter for microplates, tubes and glass vials
Analytik Jena | Biometra offers high precision adapter plates to allow a perfect fit for all kinds of tubes, vials, microplates and other different disposables.
The adapter plates are optimized for an excellent heat transfer to the disposables and enhance the uniformity over all wells and the heat up or cool down time.
The exchange of adapter plates can be performed very easily within one minute.
If you need an adapter plate for specially shaped micro plates, tubes or vials, so please send us additional specific information about your used sample container: manufacturer, description, article number.
You will receive your special formed adapter plate!
8.2 Technical specifications
BioShake XP, BioShake iQ
Adapter plates
Microplates
Tubes
Glass vials
Temperature
Regulation range
BioShake XP BioShake iQ
96-, 384- and 1536-well microplates (deep-well, v-well, square-well and u-well shape)
0.2, 0.5, 1.5 and 2.0 ml tubes, Lysis Tubes 0.5 - 2.0 ml,
15 ml and 50 ml Falcon tubes
2.0 and 4.0 ml glass vials
--- RT to 99 °C
Temperature setting
Regulation accuracy
Temperature accuracy
Heat-up time
---
---
---
---
---
0.1°C increment adjustable from 0 °C to 99 °C
± 0.1°C
± 0.5 °C at 45 °C
± 0.7 °C at 75 °C
± 1.0 °C at 95 °C
10 min from RT to 95 °C
(approx. 7 °C/min)
--- Active Cool-down time
Mixing
Mixing frequency
Mixing orbit
Speed setting resolution
Mixing regulation accuracy
Timer
Timer setting
Working / Alarm
200 to 3,000 rpm (microplates)
200 to 1,800 rpm (tubes, glass vials)
Constant 2 mm
50 rpm increments
± 25 rpm
1 min - 99 h / with automatic switch to stand-by
Continuous working / audible alarm
User manual BioShake series Issue 06/2013 19
Specification
BioShake XP BioShake iQ
Programming
Operation control
Programs
Program capacity
Display
Display
Target values/actual values
Electrical
Operating voltages
Power supply
Properties
Housing material
Operating range
Dimensions (WxDxH) [mm]
Weight [kg]
Micro controller
2 programs / definable buttons P1 and P2
3 steps / internal memory
2 x 16 digits LCD-display with backlight (black)
Time, mixing frequency Time, mixing frequency, temperature
24 V DC input / 100 Watt
External power supply unit / 100-240 V AC, 50-60 Hz
Aluminum anodized, silver
+5 °C to 45 °C (80 % max. relative humidity)
142 x 170 x 80
2.0 2.7
Technical specifications are subject to change!
20 Issue 06/2013 User manual BioShake series
Ordering information
9 Ordering information
Product Order – No.
BioShake XP (100 – 240V, EU plug); without adapter plate
BioShake XP (100 – 240 V, US plug); without adapter plate
BioShake XP (100 – 240 V, Japan plug); without adapter plate
BioShake iQ (100 – 240V, EU plug); without adapter plate
BioShake iQ (100 – 240 V, US plug); without adapter plate
BioShake iQ (100 – 240 V, Japan plug); without adapter plate
ADAPTER* for MICROPLATES
Microplate adapter – Flat bottom
Microplate adapter –flat bottom, high base, universal
Microplate adapter – 96 well round bottom, universal
Microplate adapter – 96 well standard PCR plate, universal
Microplate adapter – 384 well standard PCR plate, universal
ADAPTER* for DEEP WELL PLATES
Adapter – Eppendorf deep well plate 96/1000 µl
Adapter – Eppendorf Deep well Plate 96/500 µl
ADAPTER* for TUBES
Adapter – 24 x 2.0 ml or 15 x 0.5 ml tubes
Adapter – 24 x 1.5 ml or 15 x 0.5 ml tubes
Adapter – 40 x 0.5 ml or 28 x 0.2 ml tubes
Adapter – 96 x 0.2 ml tubes
Adapter – 35 x 2.0 ml or 24 x 0.5 ml tubes
Adapter – 35 x 1.5 ml or 24 x 0.5 ml tubes
Adapter – 35 x Lysis tubes 0.5 – 2.0 ml
ADAPTER* for FALCON TUBES
Adapter-Falcon tubes – 4 x 50 ml or 2 x 15 ml
Adapter-Falcon tubes – 12 x 15 ml
ADAPTER* for GLASS VIALS
Adapter – 30 x 2.0 ml glass vials
Adapter – 20 x 4.0 ml glass vials
CUSTOMIZED ADAPTERS*
Customized adapters for specifically shaped microplates, tubes or vials (on request)
* All Adapters are delivered incl. 3 fixation screws and screwdriver
848-1808-0505
848-1808-0555
848-1808-0565
848-1808-0506
848-1808-0556
848-1808-0566
848-1808-1021
848-1808-1022
848-1808-1031
848-1808-1041
848-1808-1051
848-1808-1121
848-1808-1131
848-1808-1061
848-1808-1062
848-1808-1063
848-1808-1064
848-1808-1065
848-1808-1066
848-1808-1067
848-1808-1093
848-1808-1094
848-1808-1071
848-1808-1072
848-1808-1000
User manual BioShake series Issue 06/2013 21
Ordering information
Product
Spare Parts
Power supply for BioShake XP
(100 – 240 V AC, 50 – 60 Hz, 24 V DC, 60 W)
Power supply for BioShake iQ
(100 – 240 V AC, 50 – 60 Hz, 24 V DC, 108 W)
Power cord (Europe)
Power cord (USA)
Power cord (Japan)
Fixation screws for Security Cover Plate or Adapter (3 pcs.)
Fixation springs for BioShake XP/iQ (8 pcs.)
Security Cover Plate (incl. 3 fixation screws and tool)
Order – No.
848-1808-9010
848-1808-9011
848-1808-9101
848-1808-9110
848-1808-9111
848-1808-9006
848-1808-9007
848-1808-9008
Please use the original accessories recommended by Analytik Jena | Biometra.
Using spare parts or disposables which we have not recommended can reduce the precision, accuracy and life of the BioShake. We do not honour any warranty or accept any responsibility for damage resulting from such action.
22 Issue 06/2013 User manual BioShake series
Ordering information
Fig. 9-1 BioShake adapters
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Maintenance and repair
10 Maintenance and repair
10.1 Cleaning and maintenance
The device is maintenance-free. Before cleaning the BioShake, disconnect the power cord and make sure that the temperature at the contact surface is below +40 °C.
If contaminated the device may be cleaned using a mild soap solution and water or an alcohol-based disinfectant.
Do not immerse the unit in liquid.
10.2 Servicing
Regular servicing is not necessary. Should it become necessary to repair the equipment, please contact our Service Department.
10.3 Replacement of spare parts
Only original spare parts mentioned in these operating instructions are allowed.
10.4 Other accessories
There are no accessory parts for this instruments.
24 Issue 06/2013 User manual BioShake series
Service
11 Service
Should you have any problems with this unit, please contact our service department or your local Analytik Jena | Biometra distributor.
Analytik Jena AG
Service Department Life Science
Konrad-Zuse-Straße 1
D-07745 Jena
Tel.: +49 (0) 36 41 / 77-94 11
Fax: +49 (0) 36 61 / 77-76 77 76 e-mail: [email protected]
Internet: www.bio.analytik-jena.com
Attention!
If you would like to send the unit back to us, please read the following instructions.
11.1 Instructions for return shipment
•
Return only defective devices. For technical problems which are not definitively recognisable as device faults please contact the technical service department at
Analytik Jena Life Science (Tel.: +49 (0) 36 41 / 77-94 11, Fax: +49 (0) 36 61 / 77-
76 77 76, e- mail: [email protected])
•
Please contact our service department for providing a return material
authorization number (RMA). This number has to be applied clearly visible to the outer box. Returns without the RMA will be not be accepted!
Important!
Carefully clean all parts of the instrument from residues, and of biologically dangerous, chemical or radioactive contaminants. If an instrument is contaminated, Analytik Jena |
Biometra will be forced to refuse to accept the device. The sender of the repair order will be held liable for possible damages and losses resulting from insufficient decontamination of the device.
• Please prepare written confirmation (use the “Equipment Decontamination
Declaration” following on the next page) that the device is free of biologically dangerous, chemical or radioactive contaminants. This confirmation must be attached to the outside of the packaging.
• Use the original packing or a similarly robust packing when returning the device. If not available, contact Analytik Jena | Biometra or your local distributor.
•
Label the outside of the box with “CAUTION! SENSITIVE INSTRUMENT!” and the
RAN number sticker. Attach the Decontamination Declaration!
• Please enclose a note which contains the following: a) Sender’s name and address, b) Name of a contact person for further inquiries with telephone number. c) Precise description of the fault, which also reveals during which procedures the fault occurred, if possible.
User manual BioShake series Issue 06/2013 25
Equipment Decontamination Certificate
12 Equipment Decontamination Certificate
To enable us to comply with german law (i.e. §71 StrlSchV, §17 GefStoffV and §19
ChemG) and to avoid exposure to hazardous materials during handling or repair, please complete this form, prior to the equipment leaving your laboratory.
COMPANY / INSTITUTE
ADRESS
PHONE NO.
EQUIPMENT MODEL
FAX NO.
SERIAL NO.
If on loan / evaluation Start date:
Hazardous material used with this equipment:
Finish date:
Method of cleaning / decontamination:
SIGNED
(HEAD OF DIV./ DEP./ INSTITUTE / COMPANY)
DATE
The equipment has been cleaned and decontaminated:
Name Position
PLEASE RETURN THIS FORM TO ANALYTIK JENA | BIOMETRA OR YOUR LOCAL
ANLYTIK JENA | BIOMETRA DISTRIBUTOR TOGETHER WITH THE EQUIPMENT.
PLEASE ATTACH THIS CERTIFICATE OUTSIDE THE PACKAGING. INSTRUMENTS
WITHOUT THIS CERTIFICATE ATTACHED WILL BE RETURNED TO SENDER.
General Information for Decontamination:
Please contact your responsible health & safety officer for details.
Use of radioactive substances:
Please contact your responsible person for details.
Use of genetically change organism or parts of those:
Please contact your responsible person for details.
26 Issue 06/2013 User manual BioShake series
Note for disposal of electric/electronic waste
13 Note for disposal of electric/electronic waste
This symbol (the crossed-out wheelie bin) means, that this product should be brought to the return systems and/or separate systems available to end-users according to yours country regulations, when this product has reached the end of its lifetime!
For details, please contact your local distributor!
This symbol applies only to the countries within the EEA*.
*EEA = European Economics Area, comprising all EU-members plus Norway, Iceland and Liechtenstein.
User manual BioShake series Issue 06/2013 27
EC - Declaration of Conformity
EU - Konformitätserklärung
14 EC - Declaration of Conformity
EU - Konformitätserklärung
May 2011 im Sinne der EG-Richtlinie über elektrische Betriebsmittel zur Verwendung innerhalb bestimmter Spannungsgrenzen 2006/95/EG
following the EC directive about electrical equipment for use within certain limits of voltage 2006/95/EC
und / and im Sinne der EG-Richtlinie für die elektromagnetische Verträglichkeit 2004/108/EG.
following the EC directive about the electromagnetic compatibility 2004/108/EC.
Hiermit erklären wir, dass folgende Shaker
Herewith we declare that the following Shakers
Typ / type: BioShake XP, BioShake iQ,
Best.-Nr. / Order No. 848-1808-0505, 848-1808-0555, 848-1808-0565,
848-1808-0506, 848-1808-0556, 848-1808-0566 den grundlegenden Anforderungen der
corresponds to the basic requirements of
EG-Niederspannungsrichtlinie 2006/95/EG und der
EC low voltage directive 2006/95/EC and the
EG-Richtlinie über die elektromagnetische Verträglichkeit 2004/108/EG entsprechen.
EC directive about the electromagnetic compatibility 2004/108/EC.
Quality Manager
28 Issue 06/2013 User manual BioShake series
Warranty
15 Warranty
This laboratory instrument is produced with the highest practical standards of materials, workmanship, and design. The design and manufacture of parts have been conceived with one purpose - to produce units which will give satisfactory service.
Analytik Jena | Biometra guarantees this unit to be free from defects in materials or workmanship under normal use or service for 24 month from date of shipment.
If, during this time, this unit proves defective in materials or workmanship, Analytik Jena |
Biometra will repair or replace it free of charge if returned to us prepaid.
This guarantee does not cover damage in transit, damage caused by carelessness, misuse or neglect, or unsatisfactory performance as a result of conditions beyond our control; or consequential losses as a result of failure of our product.
Analytik Jena AG
Life Science
Konrad-Zuse-Straße 1
D-07745 Jena
Tel: +49 (0) 36 41 / 77-94 00
Fax: +49 (0) 36 61 / 77-76 77 76 e-mail: [email protected]
Internet: www.bio.analytik-jena.com
Biometra Service Department
Rudolf-Wissell-Str. 14-16
D-37079 Göttingen
Tel.: +49 (0)5 51 50 68 6-10 or -12
Fax.: +49 (0)5 51 50 68 6-11 e-mail: [email protected]
Internet: www.bio.analytik-jena.com
User manual BioShake series Issue 06/2013 29
Table of figures
16 Table of figures
Fig. 1-1 Correlation between mixing frequencies and mixing effects in dependence on the filling level ............................................................................................. 3
Fig. 3-1 BioShake iQ (1) with power supply (2) and power cord (3) ............................. 8
Fig. 3-2 BioShake XP (1) with power supply (2) and power cord (3) ............................ 8
Fig. 4-1 Connections at the rear side of device (BioShake iQ and BioShake XP) ........ 9
Fig. 5-1 Mounting of adapter plates ............................................................................ 10
Fig. 6-1 Control panel of BioShake XP ....................................................................... 11
Fig. 6-2 Control panel of BioShake iQ ........................................................................ 12
Fig. 6-3 Standard functions (only for BioShake iQ and BioShake XP) ....................... 12
Fig. 6-4 Required mixing speeds depending on filling volume of tubes ...................... 13
Fig. 6-5 Required mixing speeds depending on filling volume of microplates ............ 13
Fig. 6-6 Recommended maximum mixing speeds ...................................................... 14
Fig. 6-7 Mixing speed classification of adapters ......................................................... 14
Fig. 6-8 Programming BioShake iQ or BioShake XP .................................................. 16
Fig. 9-1 BioShake adapters ........................................................................................ 23
User manual BioShake series Issue 06/2013 31
Content
3
3.1
3.2
4
5
6
6.5
6.6
6.7
6.8
6.1
6.2
6.3
6.4
6.9
6.10
6.11
7
8
8.1
8.2
9
10
10.1
10.2
10.3
10.4
Content
2
2.1
2.2
2.3
1
1.1
1.2
Introduction ............................................................................. 3
Field of application ........................................................................................... 3
Information on the use of this manual .............................................................. 4
Safety instructions .................................................................. 5
Warning and safety symbols on the BioShake ................................................. 5
Safety and caution information ......................................................................... 5
General information .......................................................................................... 7
Delivery parts .......................................................................... 8
BioShake iQ ..................................................................................................... 8
BioShake XP .................................................................................................... 8
Installation ............................................................................... 9
Changing of adapter plates .................................................. 10
Operation ............................................................................... 11
Control panel of BioShake XP ........................................................................ 11
Control panel of BioShake iQ ......................................................................... 12
Standard functions ......................................................................................... 12
Set up time ..................................................................................................... 13
Set up mixing speed ....................................................................................... 13
Set up temperature......................................................................................... 15
Short mix ........................................................................................................ 15
Start / Stop ..................................................................................................... 15
Programming .................................................................................................. 15
Set up the time counting and temperature control mode ................................ 16
Set up the buzzer and programming control mode ........................................ 17
Tips for shaker operation ..................................................... 18
Specification ......................................................................... 19
Adapter for microplates, tubes and glass vials ............................................... 19
Technical specifications ................................................................................. 19
Ordering information ............................................................ 21
Maintenance and repair ........................................................ 24
Cleaning and maintenance ............................................................................. 24
Servicing ........................................................................................................ 24
Replacement of spare parts ........................................................................... 24
Other accessories .......................................................................................... 24
User manual BioShake series Issue 06/2013 1
Content
11
11.1
12
13
14
15
16
Service ................................................................................... 25
Instructions for return shipment ...................................................................... 25
Equipment Decontamination Certificate ............................. 26
Note for disposal of electric/electronic waste .................... 27
EC - Declaration of Conformity ............................................ 28
Warranty ................................................................................ 29
Table of figures ..................................................................... 31
2 Issue 06/2013 User manual BioShake series
Introduction
1 Introduction
1.1 Field of application
The BioShake Series is special designed for precise control of speed and temperature in an apparatus with a footprint not bigger than 2 microplates side-by-side.
The apparatus offer an ultra-efficient, 3-dimensional shaking axis to mix samples in tubes, glass vials or microplates (up to 1536 wells) without the need of spinning down the samples after mixing. Even high density plates or low samples volumes are not a problem for the Shaker.
BioShake mixers have an orbit diameter of 2.0 mm and a high maximum shaking frequency of 3,000 rpm, allowing any model to easily mix standard and low volume samples in tubes, glass vials and 96-well, 384-well or 1536-well microplates.
The apparatus are working very quiet due to a special patent pending dampening system which relies upon ingenious counter-weight symmetry. They shake tubes to mix their content but do not put vibrations on the entire work bench or robotic deck.
Reduced mass sample blocks provide for quick heating and accurate temperature control across the whole block.
Fig. 1-1 Correlation between mixing frequencies and mixing effects in dependence on the filling level
User manual BioShake series Issue 06/2013 3
Information
1.2 Information on the use of this manual
The following warning and information symbols are used in this manual:
Danger! Dangerous touch voltage!
Caution!
Warning messages alert you to a specific procedure or practice which, if not followed correctly, could cause personal injury.
Caution! Hot surface!
Contact with the surface can cause burns.
Attention!
Caution messages refer you to procedures which, if not observed, could result in damage to the equipment.
Disconnect mains plug!
Note
This information must be observed to ensure correct function of the device.
The following systematic approach is introduced in the manual:
•
The chapters and figures are numbered consecutively.
•
Each image has its own caption.
•
A cross reference is indicated with an arrow (e.g. → Information on the use of this manual pg. 4).
•
Working steps are numbered.
4 Issue 06/2013 User manual BioShake series
Safety instructions
2 Safety instructions
Read through this chapter carefully prior to commissioning the BioShake Series for your own safety and for trouble-free operation.
Follow all the safety instructions explained in the manual, as well as all messages and information displayed on the screen by the internal microcomputer.
2.1 Warning and safety symbols on the BioShake
The following warning and safety symbols are affixed on the BioShake:
Caution! Hot surface!
Contact with the surface can cause burns!
This symbol is affixed on the basis unit next to the heating surface of the block (only
BioShake iQ).
Disconnect mains plug!
This symbol is affixed on the rear of the device next to the mains input.
2.2 Safety and caution information
1. Do not operate the device in rooms where work is being carried out with explosive substances.
2.
Do not use this device to process any explosive, radioactive or highly reactive substances.
3.
Do not use this device to process any substances, which could create an explosive atmosphere.
4.
Danger! Electric shock from damage to device/power cable. Danger! Lethal voltages inside the device.
5.
Warning! General hazard point.
6.
Warning! Injury from rapidly rotating holder. Injury from rapidly rotating imbalance compensation. Injury from flying tubes and plates.
7.
Warning! Injury from sample material being flung out. Injury from incorrect vortexing.
8.
Warning! Damage due to incorrect power supply.
User manual BioShake series Issue 06/2013 5
Safety instructions
9. Caution! Damage to the display from mechanical pressure. Severe vibration.
10.
Caution! Damage to electronic components caused by spilled liquids. Damage to electronic components from condensation.
11.
Caution! Poor safety due to missing operating manual. Caution when using aggressive chemicals.
12.
Please start even with minimal mixing frequencies to avoid overloading.
13. Only mix in sealed tubes and plates. Sample material can be flung out of open, inadequately sealed or unstable tubes and plates.
14. When working with hazardous, toxic and pathogenic samples, always comply with the nationally specified safety environment.
15.
Please use the original power supply delivered by Analytik Jena | Biometra.
16.
Please use the original accessories recommended by Analytik Jena | Biometra.
Pay particular attention to personal safety gear (gloves, clothing, glasses etc.), the extraction hood and the safety class of the laboratory.
Read this section carefully and follow the instructions before beginning installation and any procedure.
The unit must be placed on a horizontal solid work space.
The device must be saving from shocks and falling.
The unit must be placed in sufficient distance to heat registers or radiators to ensure ambient temperature conditions in accordance with the technical specifications (section 8.2).
Use the normal care and precaution one would use with any electrical appliance.
Be sure to use a power cord with the same rating and of the same type (three-wire with neutral conductor) as the one supplied with the instrument.
Ensure that voltage and frequency of your power source match the input voltage and frequency inscribed on the electrical rating label of the power supply (AC/DC adaptor) and that the output voltage matches the rating of the equipment. The AC/DC adaptor is designed to work with a single-phase power system having a grounded neutral conductor.
If you are not sure what type of power is supplied to your building contact your facilities manager.
As BioShake is an electrical device do not spill large quantities of liquid onto the device. Do not use it in an explosive atmosphere.
As parts of the device may generate electric, magnetic or electromagnetic fields, keep parts away that may be affected (e.g. data storage units).
6 Issue 06/2013 User manual BioShake series
Safety instructions
BioShake is capable of heating the plate surface up to 100 °C.
This temperature is high enough to cause serious burns if touched.
Use extreme caution at all times.
Never leave your unit accessible to others when it is hot.
Never touch the plate surface unless you are sure it is cold.
Use oven gloves if necessary.
Do not unscrew any part of BioShake except for the interchangeable power supply, the power cord and different blocks.
Do not make any mechanical or electrical modifications to the equipment.
Never do anything else with BioShake as intended in this manual.
Noncompliance of the safety instructions may lead to device damage, loss of warranty and may cause serious personal injury as well as death.
2.3 General information
Warning! Injury from sample material being flung out.
Sample material can be flung out of open, inadequately sealed or unstable wells, plates and tubes.
•
Only mix in sealed tubes and plates
•
When working with hazardous, toxic and pathogenic samples, always comply with nationally specified safety environment. Pay particular attention to personal safety gear (gloves, clothing, glasses etc.), the extraction hood and the safety class of the laboratory.
When setting mixing frequency, adhere to the rules outlined in
Fig. 6-4 to 6-7.
User manual BioShake series Issue 06/2013 7
Delivery parts
3 Delivery parts
3.1 BioShake iQ
(3)
1 BioShake iQ
2 Power supply
3 Power cord
Operating manual (no picture)
(1)
(1)
(2)
(1)
Fig. 3-1 BioShake iQ (1) with power supply (2) and power cord (3)
3.2 BioShake XP
(3)
1 BioShake iQ
2 Power supply
3 Power cord
Operating manual (no picture)
(1)
(2)
Fig. 3-2 BioShake XP (1) with power supply (2) and power cord (3)
8 Issue 06/2013 User manual BioShake series
Installation
4 Installation
Unpack and carefully check the instrument. Report any damage or missing items to your local distributor.
If no damage is found place the device up on a stable horizontal surface.
Fig. 4-1 Connections at the rear side of device (BioShake iQ and BioShake XP)
Before starting to work with the BioShake:
Remove the cover plate before installing an adapter plate:
Loose the 3 torx screws with the torx screwer at the adapter packaging and remove the cover plate.)
If the adapter will not be removed before installing an adapter, the adapter will not be recognized by the device!
Place an adapter on the shaker (chapter 5: Changing of adapter plates).
Plug the external power supply (2) into the 24 V socket at the rear side of the BioShake iQ or BioShake XP.
Plug the power cable (3) into the power supply (2) and into the wall socket.
Turn On the instrument.
It is advisable to carry out a test run at maximum speed to ensure that the device does not move while mixing.
User manual BioShake series Issue 06/2013 9
Changing of adapter plates
5 Changing of adapter plates
To change the adapter plates, please follow the subsequent steps:
Attention!
If the security cover plate or an adapter plate already be mounted, please remove it first!
1. Turn off the instrument!
2. Remove all sample carriers B-3 (tubes, vials, microplates etc.).
3. Loosen the 3 torx screws A-1, A-2 and A-3 by using the supplied screwdriver
(Torx size 8, rotate left).
4. Take off the cover clate or adapter plate B-2 straight up and put it on a clean, soft surface.
Mounting of the new adapter plate:
1. Please take care to a clean, dirt-free and particle-free operation.
2. Insert the new adapter plate straight into the impression of the plate holder B-1.
3. Check the fixed position.
4. Fix all torx screws A-1, A-2 und A-3 using clockwise rotation. Please take care to uniform controlled tightening of screws to ensure a good fit.
5. Please take care to good and tight fit of sample carriers B-3 (tubes, vials, microplates etc.).
6. Turn on the instrument! BioShake will recognize the different block types. All pending information and indications are shown in the display.
B-3
B-2 A-2
A-3
A-1
10
B-1
Fig. 5-1 Mounting of adapter plates
Attention!
Heavier blocks may limit the shaking speed (see Fig. 6-6 Recommended maximum mixing speeds)
Issue 06/2013 User manual BioShake series
Operation
6 Operation
The BioShake is designed for shaking (BioShake iQ and BioShake XP) and temperature control (BioShake iQ) of liquids in tissue culture plates, microplates, tubes and glass vials. Microplates or tubes have to be placed on the shaker platform of the BioShake.
The liquid to be shaken is introduced into the wells or tubes.
The automatic start up function of the unit ensures a gradual start of the shaking process without sample splashing.
The drive is absolutely wear-free and maintenance-free.
6.1 Control panel of BioShake XP
The following picture shows the control panel of the BioShake XP.
Fig. 6-1 Control panel of BioShake XP
User manual BioShake series Issue 06/2013 11
Operation
6.2 Control panel of BioShake iQ
Fig. 6-2 Control panel of BioShake iQ
6.3 Standard functions
12
Fig. 6-3 Standard functions (only for BioShake iQ and BioShake XP)
Issue 06/2013 User manual BioShake series
Operation
6.4 Set up time
Use time cursors to set the required operation time.
If the timer is not selected, pressing the Start / Stop will cause a continuous operation of the shaker.
6.5 Set up mixing speed
It is possible to control mixing speeds from 200 rpm up to 3,000 rpm.
To set the mixing speeds use the mix buttons.
Required mixing speeds [rpm] for different tubes depending on
filling volume [%] for diluted fluids
Filling volume 0.2 ml tubes 0.5 ml tubes
25 % 1400 - 1800 1200 - 1600
1.5 ml tubes
1000 - 1300
2.0 ml tubes
1000 - 1300
50 %
75 %
1200 - 1500
1000 - 1300
1100 - 1300
1000 - 1200
1000 - 1200
900 - 1100
900 - 1200
900 - 1100
Fig. 6-4 Required mixing speeds depending on filling volume of tubes
Filling volume
10 %
Required mixing speeds [rpm] for microplates depending on filling
volume/well [%] for diluted fluids
96-well
(standard)
1800 - 2200
384-well
(standard)
2200 - 2600
384-well
(small-vol.)
2800 - 3000
1536-well
(standard)
2800 - 3000
25 %
50 %
75 %
1600 - 2000
1400 - 1800
1200 - 1600
2000 - 2400
1800 - 2200
1600 - 2000
2400 - 3000
2200 - 2600
2000 - 2400
2600 - 3000
2400 - 2600
2200 - 2600
Fig. 6-5 Required mixing speeds depending on filling volume of microplates
User manual BioShake series Issue 06/2013 13
Operation
14
Type
Microplate
Deep well plate
Deep well plate
Deep well plate
Maximal mixing speeds [rpm] for microplates depending on the
weight [g]
Weight
0 -80 g
0 -120 g
0 -150 g
150 -300 g
1,400
x
2,000
x
2,500
x
3,000
x
Fig. 6-6 Recommended maximum mixing speeds
Adapter categories:
All adapters will be automatically detected while mounting on the instrument. The allocation of the adapter to a particular category determines the properties.
Adapter category
Max. rpm
Cat 01
Cat 02
Cat 03
Cat 04
Cat 05
Cat 06
Cat 07
Cat 10
3,000
1,800
2,200
3,000
1,000
----
2,500
OEM
Available adapters
Item no. Description
-
848-1808-1061
848-1808-1062
A) Without adapter (heating function not available)
B) All non classified adapters (heating function not available)
Adapter for 24x 2.0 ml or 15x 0.5 ml tubes
Adapter for 24x 1.5 ml or 15x 0.5 ml tubes
848-1808-1063
848-1808-1065
848-1808-1066
848-1808-1067
848-1808-1071
848-1808-1072
848-1808-1121
848-1808-1131
848-1808-1041
848-1808-1064
Adapter for 40x 0.5 ml or 28x 0.2 ml tubes
Adapter for 35x 2.0 ml or 24x 0.5 ml tubes
Adapter for 35x 1.5 ml or 24x 0.5 tubes
Adapter for 35x Lysis tubes 0.5 – 2.0 ml
Adapter for 30x 2.0 ml glass vials
Adapter for 20x 4.0 ml glass vials
Deep well plate adapter – 96 well / 1,000 µl (Eppendorf)
Deep well plate adapter - 96 well / 500 µl (Eppendorf)
Microplate adapter - 96 well standard PCR plate, universal
Adapter for 96x 0.2 ml tubes
Microplate adapter - flat bottom 848-1808-1021
848-1808-1031
848-1808-1093
Microplate adapter (universal) - 96 well round bottom
Adapter-Falcon tubes – 4 x 50 ml or 2 x 15 ml
848-1808-1094 Adapter-Falcon-tubes – 12 x 15 ml
Not available at the time
848-1808-1022 Microplate adapter - flat bottom, high base
848-1808-1051
Customer specified
Microplate adapter - 384 well standard PCR plates
Fig. 6-7 Mixing speed classification of adapters
Issue 06/2013 User manual BioShake series
Operation
All pending information and indications are shown in the display.
Display Line 1
Adapter Cat.: 01
Display Line 2
Max. Speed: 3000
6.6 Set up temperature
It is possible to control temperature from room temperature up to 99 °C.
To set the temperature use the Temp Buttons.
Attention!
Microplates melt at extremely high temperatures.
Only heat microplates made of polystyrene to a maximum of 70 °C
6.7 Short mix
By pressing Short Mix, the shaker will start up with the set parameters. Releasing the Short Mix button will stop the shaker.
6.8 Start / Stop
By pressing Start / Stop, the shaker will start up with the set parameters.
If you press Start / Stop again, the shaker will stop.
6.9 Programming
With the BioShake iQ and BioShake XP it is possible to have 2 automatic programs.
Both programs can have 3 different steps with different time, speed and temperature settings for each step.
User manual BioShake series Issue 06/2013 15
Operation
Program the BioShake iQ or BioShake XP with the following steps:
16
Programming BioShake iQ or BioShake XP Fig. 6-8
6.10 Set up the time counting and temperature control mode
2.
The time counting function enables temperature control and/or mixing for a limited period of time. There are two ways to measure the time of the temperature control processes.
Time control:
Temp control:
Time counting begins immediately when “Start/Stop” is pressed.
The Temp control mode is the standard mode. Time counting only starts when the nominal temperature value has been reached.
To terminate the process earlier, press “Start/Stop” again.
Switch the TCMo mode with the following steps:
1.
+
To start press Temp(+) and P1 and hold for at least
3 seconds.
Display Line 1
Display Line 2
TCMo BMo -
Temp auto
By pressing Time(+) or Time(-) the shaker will switch between
Temp mode or Time mode.
3.
+
To finish press P1 and Temp(+) and hold for at least 3 seconds.
Issue 06/2013 User manual BioShake series
Operation
6.11 Set up the buzzer and programming control mode
The programming control mode enables buzzer and temperature control at the end of automatic programs. There are two ways to set up the buzzer and temperature control process.
auto mode:
User mode:
At the end of automatic programs:
At the end of automatic programs:
•
•
3x short buzzer signals
Mixing is stopped
•
Temperature control is stopped and switch off
•
Short buzzer signal every 10 seconds
•
Mixing is stopped
•
Temperature control is enabled and keep target temperature constant
Switch the BMo mode with the following steps:
1.
+
To start press Temp(+) and P1 and hold for at least
3 seconds.
2.
Display Line 1
TCMo BMo -
Display Line 2
Temp auto
By pressing Mix(+) or Mix(-) the shaker will switch between
auto mode or user mode.
3.
+
To finish press P1 and Temp(+) and hold for at least 3 seconds.
User manual BioShake series Issue 06/2013 17
Tips for shaker operation
7 Tips for shaker operation
A wide variety of well plates are commercially available.
To ensure that the plates are positioned securely in the plate holder, they must correspond with the ANIS/SBS Standard for
Microplates.
If the filled plate has a weight of more than 80 g, then the maximum rated shaking frequency of 3,000 rpm may not be attainable with a safety stand. Select a lower shaking frequency in this case. However, there is no risk of damage to the BioShake – even if the weight is too high then the shaking action stops due to excessive weight or excessive shaking frequencies. As soon as you reduce the frequency, the unit will stand firm.
Maximum shaker platform load: 500 g (filled well plate).
The shaker is driven by a maintenance-free brushless motor which enables silent operation and constant shaking speed independent of the load.
IMPORTANT NOTES:
• Please start even with minimal mixing frequencies to avoid overloading.
• Only mix in sealed tubes and plates. Sample material can be flung out of open, inadequately sealed or unstable tubes and plates.
• When working with hazardous, toxic and pathogenic samples, always comply with the nationally specified safety environment.
• Pay particular attention to personal safety gear (gloves, clothing, glasses etc., the extraction hood and the safety class of the laboratory.
18 Issue 06/2013 User manual BioShake series
Specification
8 Specification
8.1 Adapter for microplates, tubes and glass vials
Analytik Jena | Biometra offers high precision adapter plates to allow a perfect fit for all kinds of tubes, vials, microplates and other different disposables.
The adapter plates are optimized for an excellent heat transfer to the disposables and enhance the uniformity over all wells and the heat up or cool down time.
The exchange of adapter plates can be performed very easily within one minute.
If you need an adapter plate for specially shaped micro plates, tubes or vials, so please send us additional specific information about your used sample container: manufacturer, description, article number.
You will receive your special formed adapter plate!
8.2 Technical specifications
BioShake XP, BioShake iQ
Adapter plates
Microplates
Tubes
Glass vials
Temperature
Regulation range
BioShake XP BioShake iQ
96-, 384- and 1536-well microplates (deep-well, v-well, square-well and u-well shape)
0.2, 0.5, 1.5 and 2.0 ml tubes, Lysis Tubes 0.5 - 2.0 ml,
15 ml and 50 ml Falcon tubes
2.0 and 4.0 ml glass vials
--- RT to 99 °C
Temperature setting
Regulation accuracy
Temperature accuracy
Heat-up time
---
---
---
---
---
0.1°C increment adjustable from 0 °C to 99 °C
± 0.1°C
± 0.5 °C at 45 °C
± 0.7 °C at 75 °C
± 1.0 °C at 95 °C
10 min from RT to 95 °C
(approx. 7 °C/min)
--- Active Cool-down time
Mixing
Mixing frequency
Mixing orbit
Speed setting resolution
Mixing regulation accuracy
Timer
Timer setting
Working / Alarm
200 to 3,000 rpm (microplates)
200 to 1,800 rpm (tubes, glass vials)
Constant 2 mm
50 rpm increments
± 25 rpm
1 min - 99 h / with automatic switch to stand-by
Continuous working / audible alarm
User manual BioShake series Issue 06/2013 19
Specification
BioShake XP BioShake iQ
Programming
Operation control
Programs
Program capacity
Display
Display
Target values/actual values
Electrical
Operating voltages
Power supply
Properties
Housing material
Operating range
Dimensions (WxDxH) [mm]
Weight [kg]
Micro controller
2 programs / definable buttons P1 and P2
3 steps / internal memory
2 x 16 digits LCD-display with backlight (black)
Time, mixing frequency Time, mixing frequency, temperature
24 V DC input / 100 Watt
External power supply unit / 100-240 V AC, 50-60 Hz
Aluminum anodized, silver
+5 °C to 45 °C (80 % max. relative humidity)
142 x 170 x 80
2.0 2.7
Technical specifications are subject to change!
20 Issue 06/2013 User manual BioShake series
Ordering information
9 Ordering information
Product Order – No.
BioShake XP (100 – 240V, EU plug); without adapter plate
BioShake XP (100 – 240 V, US plug); without adapter plate
BioShake XP (100 – 240 V, Japan plug); without adapter plate
BioShake iQ (100 – 240V, EU plug); without adapter plate
BioShake iQ (100 – 240 V, US plug); without adapter plate
BioShake iQ (100 – 240 V, Japan plug); without adapter plate
ADAPTER* for MICROPLATES
Microplate adapter – Flat bottom
Microplate adapter –flat bottom, high base, universal
Microplate adapter – 96 well round bottom, universal
Microplate adapter – 96 well standard PCR plate, universal
Microplate adapter – 384 well standard PCR plate, universal
ADAPTER* for DEEP WELL PLATES
Adapter – Eppendorf deep well plate 96/1000 µl
Adapter – Eppendorf Deep well Plate 96/500 µl
ADAPTER* for TUBES
Adapter – 24 x 2.0 ml or 15 x 0.5 ml tubes
Adapter – 24 x 1.5 ml or 15 x 0.5 ml tubes
Adapter – 40 x 0.5 ml or 28 x 0.2 ml tubes
Adapter – 96 x 0.2 ml tubes
Adapter – 35 x 2.0 ml or 24 x 0.5 ml tubes
Adapter – 35 x 1.5 ml or 24 x 0.5 ml tubes
Adapter – 35 x Lysis tubes 0.5 – 2.0 ml
ADAPTER* for FALCON TUBES
Adapter-Falcon tubes – 4 x 50 ml or 2 x 15 ml
Adapter-Falcon tubes – 12 x 15 ml
ADAPTER* for GLASS VIALS
Adapter – 30 x 2.0 ml glass vials
Adapter – 20 x 4.0 ml glass vials
CUSTOMIZED ADAPTERS*
Customized adapters for specifically shaped microplates, tubes or vials (on request)
* All Adapters are delivered incl. 3 fixation screws and screwdriver
848-1808-0505
848-1808-0555
848-1808-0565
848-1808-0506
848-1808-0556
848-1808-0566
848-1808-1021
848-1808-1022
848-1808-1031
848-1808-1041
848-1808-1051
848-1808-1121
848-1808-1131
848-1808-1061
848-1808-1062
848-1808-1063
848-1808-1064
848-1808-1065
848-1808-1066
848-1808-1067
848-1808-1093
848-1808-1094
848-1808-1071
848-1808-1072
848-1808-1000
User manual BioShake series Issue 06/2013 21
Ordering information
Product
Spare Parts
Power supply for BioShake XP
(100 – 240 V AC, 50 – 60 Hz, 24 V DC, 60 W)
Power supply for BioShake iQ
(100 – 240 V AC, 50 – 60 Hz, 24 V DC, 108 W)
Power cord (Europe)
Power cord (USA)
Power cord (Japan)
Fixation screws for Security Cover Plate or Adapter (3 pcs.)
Fixation springs for BioShake XP/iQ (8 pcs.)
Security Cover Plate (incl. 3 fixation screws and tool)
Order – No.
848-1808-9010
848-1808-9011
848-1808-9101
848-1808-9110
848-1808-9111
848-1808-9006
848-1808-9007
848-1808-9008
Please use the original accessories recommended by Analytik Jena | Biometra.
Using spare parts or disposables which we have not recommended can reduce the precision, accuracy and life of the BioShake. We do not honour any warranty or accept any responsibility for damage resulting from such action.
22 Issue 06/2013 User manual BioShake series
Ordering information
Fig. 9-1 BioShake adapters
User manual BioShake series Issue 06/2013 23
Maintenance and repair
10 Maintenance and repair
10.1 Cleaning and maintenance
The device is maintenance-free. Before cleaning the BioShake, disconnect the power cord and make sure that the temperature at the contact surface is below +40 °C.
If contaminated the device may be cleaned using a mild soap solution and water or an alcohol-based disinfectant.
Do not immerse the unit in liquid.
10.2 Servicing
Regular servicing is not necessary. Should it become necessary to repair the equipment, please contact our Service Department.
10.3 Replacement of spare parts
Only original spare parts mentioned in these operating instructions are allowed.
10.4 Other accessories
There are no accessory parts for this instruments.
24 Issue 06/2013 User manual BioShake series
Service
11 Service
Should you have any problems with this unit, please contact our service department or your local Analytik Jena | Biometra distributor.
Analytik Jena AG
Service Department Life Science
Konrad-Zuse-Straße 1
D-07745 Jena
Tel.: +49 (0) 36 41 / 77-94 11
Fax: +49 (0) 36 61 / 77-76 77 76 e-mail: [email protected]
Internet: www.bio.analytik-jena.com
Attention!
If you would like to send the unit back to us, please read the following instructions.
11.1 Instructions for return shipment
•
Return only defective devices. For technical problems which are not definitively recognisable as device faults please contact the technical service department at
Analytik Jena Life Science (Tel.: +49 (0) 36 41 / 77-94 11, Fax: +49 (0) 36 61 / 77-
76 77 76, e- mail: [email protected])
•
Please contact our service department for providing a return material
authorization number (RMA). This number has to be applied clearly visible to the outer box. Returns without the RMA will be not be accepted!
Important!
Carefully clean all parts of the instrument from residues, and of biologically dangerous, chemical or radioactive contaminants. If an instrument is contaminated, Analytik Jena |
Biometra will be forced to refuse to accept the device. The sender of the repair order will be held liable for possible damages and losses resulting from insufficient decontamination of the device.
• Please prepare written confirmation (use the “Equipment Decontamination
Declaration” following on the next page) that the device is free of biologically dangerous, chemical or radioactive contaminants. This confirmation must be attached to the outside of the packaging.
• Use the original packing or a similarly robust packing when returning the device. If not available, contact Analytik Jena | Biometra or your local distributor.
•
Label the outside of the box with “CAUTION! SENSITIVE INSTRUMENT!” and the
RAN number sticker. Attach the Decontamination Declaration!
• Please enclose a note which contains the following: a) Sender’s name and address, b) Name of a contact person for further inquiries with telephone number. c) Precise description of the fault, which also reveals during which procedures the fault occurred, if possible.
User manual BioShake series Issue 06/2013 25
Equipment Decontamination Certificate
12 Equipment Decontamination Certificate
To enable us to comply with german law (i.e. §71 StrlSchV, §17 GefStoffV and §19
ChemG) and to avoid exposure to hazardous materials during handling or repair, please complete this form, prior to the equipment leaving your laboratory.
COMPANY / INSTITUTE
ADRESS
PHONE NO.
EQUIPMENT MODEL
FAX NO.
SERIAL NO.
If on loan / evaluation Start date:
Hazardous material used with this equipment:
Finish date:
Method of cleaning / decontamination:
SIGNED
(HEAD OF DIV./ DEP./ INSTITUTE / COMPANY)
DATE
The equipment has been cleaned and decontaminated:
Name Position
PLEASE RETURN THIS FORM TO ANALYTIK JENA | BIOMETRA OR YOUR LOCAL
ANLYTIK JENA | BIOMETRA DISTRIBUTOR TOGETHER WITH THE EQUIPMENT.
PLEASE ATTACH THIS CERTIFICATE OUTSIDE THE PACKAGING. INSTRUMENTS
WITHOUT THIS CERTIFICATE ATTACHED WILL BE RETURNED TO SENDER.
General Information for Decontamination:
Please contact your responsible health & safety officer for details.
Use of radioactive substances:
Please contact your responsible person for details.
Use of genetically change organism or parts of those:
Please contact your responsible person for details.
26 Issue 06/2013 User manual BioShake series
Note for disposal of electric/electronic waste
13 Note for disposal of electric/electronic waste
This symbol (the crossed-out wheelie bin) means, that this product should be brought to the return systems and/or separate systems available to end-users according to yours country regulations, when this product has reached the end of its lifetime!
For details, please contact your local distributor!
This symbol applies only to the countries within the EEA*.
*EEA = European Economics Area, comprising all EU-members plus Norway, Iceland and Liechtenstein.
User manual BioShake series Issue 06/2013 27
EC - Declaration of Conformity
EU - Konformitätserklärung
14 EC - Declaration of Conformity
EU - Konformitätserklärung
May 2011 im Sinne der EG-Richtlinie über elektrische Betriebsmittel zur Verwendung innerhalb bestimmter Spannungsgrenzen 2006/95/EG
following the EC directive about electrical equipment for use within certain limits of voltage 2006/95/EC
und / and im Sinne der EG-Richtlinie für die elektromagnetische Verträglichkeit 2004/108/EG.
following the EC directive about the electromagnetic compatibility 2004/108/EC.
Hiermit erklären wir, dass folgende Shaker
Herewith we declare that the following Shakers
Typ / type: BioShake XP, BioShake iQ,
Best.-Nr. / Order No. 848-1808-0505, 848-1808-0555, 848-1808-0565,
848-1808-0506, 848-1808-0556, 848-1808-0566 den grundlegenden Anforderungen der
corresponds to the basic requirements of
EG-Niederspannungsrichtlinie 2006/95/EG und der
EC low voltage directive 2006/95/EC and the
EG-Richtlinie über die elektromagnetische Verträglichkeit 2004/108/EG entsprechen.
EC directive about the electromagnetic compatibility 2004/108/EC.
Quality Manager
28 Issue 06/2013 User manual BioShake series
Warranty
15 Warranty
This laboratory instrument is produced with the highest practical standards of materials, workmanship, and design. The design and manufacture of parts have been conceived with one purpose - to produce units which will give satisfactory service.
Analytik Jena | Biometra guarantees this unit to be free from defects in materials or workmanship under normal use or service for 24 month from date of shipment.
If, during this time, this unit proves defective in materials or workmanship, Analytik Jena |
Biometra will repair or replace it free of charge if returned to us prepaid.
This guarantee does not cover damage in transit, damage caused by carelessness, misuse or neglect, or unsatisfactory performance as a result of conditions beyond our control; or consequential losses as a result of failure of our product.
Analytik Jena AG
Life Science
Konrad-Zuse-Straße 1
D-07745 Jena
Tel: +49 (0) 36 41 / 77-94 00
Fax: +49 (0) 36 61 / 77-76 77 76 e-mail: [email protected]
Internet: www.bio.analytik-jena.com
Biometra Service Department
Rudolf-Wissell-Str. 14-16
D-37079 Göttingen
Tel.: +49 (0)5 51 50 68 6-10 or -12
Fax.: +49 (0)5 51 50 68 6-11 e-mail: [email protected]
Internet: www.bio.analytik-jena.com
User manual BioShake series Issue 06/2013 29
Table of figures
16 Table of figures
Fig. 1-1 Correlation between mixing frequencies and mixing effects in dependence on the filling level ............................................................................................. 3
Fig. 3-1 BioShake iQ (1) with power supply (2) and power cord (3) ............................. 8
Fig. 3-2 BioShake XP (1) with power supply (2) and power cord (3) ............................ 8
Fig. 4-1 Connections at the rear side of device (BioShake iQ and BioShake XP) ........ 9
Fig. 5-1 Mounting of adapter plates ............................................................................ 10
Fig. 6-1 Control panel of BioShake XP ....................................................................... 11
Fig. 6-2 Control panel of BioShake iQ ........................................................................ 12
Fig. 6-3 Standard functions (only for BioShake iQ and BioShake XP) ....................... 12
Fig. 6-4 Required mixing speeds depending on filling volume of tubes ...................... 13
Fig. 6-5 Required mixing speeds depending on filling volume of microplates ............ 13
Fig. 6-6 Recommended maximum mixing speeds ...................................................... 14
Fig. 6-7 Mixing speed classification of adapters ......................................................... 14
Fig. 6-8 Programming BioShake iQ or BioShake XP .................................................. 16
Fig. 9-1 BioShake adapters ........................................................................................ 23
User manual BioShake series Issue 06/2013 31
Advertisement
Key features
- Precise control of speed and temperature
- 3-dimensional shaking axis
- Mixing of samples in tubes, glass vials or microplates
- Temperature control from room temperature up to 99 °C
- Two automatic programs with three steps
- Quiet operation
