Q-Cycler II - Denville Scientific Inc.

Q-Cycler II - Denville Scientific Inc.
ExpressGene
P5200
Instruction Manual
Release Information
This document is protected by copyright. All Rights Reserved.
Quanta Biotech Ltd
Unit 4 Byfleet Technical Centre
Canada Road
Byfleet
Surrey
KT14 7JX
United Kingdom
Phone +44 (0) 1932 344550 • Fax +44 (0) 1932 353108
Email: [email protected]
Web: www.Quantabiotech.com
Manual Version:
1.0
Trademark Information
All product and brand names used in this document are trademarks or
registered trademarks of their respective holders.
Copyright © 2010 Quanta Biotech Ltd
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TABLE OF CONTENTS
General Information ...........................................................................................1
Policy Statement..............................................................................................1
Warranty Statement .........................................................................................1
1
Safety Warnings and Precautions .........................................................2
1.1 What you should know ..............................................................................2
1.1.1
Instrument Safety ......................................................................2
1.1.2
Symbols and Conventions ........................................................2
1.1.3
Potential Safety Hazard ............................................................3
2
Installation...............................................................................................6
2.1 Requirements ...........................................................................................6
2.1.1
Site ...........................................................................................6
2.1.2
Electrical ...................................................................................6
2.1.3
Thermal Block ...........................................................................7
2.2 Unpacking the Thermal Cycler .................................................................8
2.2.1
Package Contents.....................................................................8
2.2.2
Initial Inspection ........................................................................8
2.3 Functional Overview .................................................................................9
2.3.1
Networking the Thermal Cycler ...............................................10
2.4 AC Power Connection.............................................................................11
2.4.1
Operating Power Requirements ..............................................11
2.4.2
Fuses ......................................................................................11
2.4.3
Grounding Requirements ........................................................11
3
Software Operation ...............................................................................13
3.1 Required Computer Skills .......................................................................13
3.2 Start Screen............................................................................................13
3.3 Administrator Settings .............................................................................15
3.3.1
Cycler Management ................................................................15
3.3.2
User Management ..................................................................17
4
Programming ........................................................................................21
4.1 Creating PCR Programs .........................................................................21
4.1.1
DND Programming..................................................................21
4.1.2
Program Wizard ......................................................................27
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4.2 General Programming Guidelines .......................................................... 28
4.3 Editing Programs ................................................................................... 29
4.3.1
Editing Program Parameters .................................................. 29
4.3.2
Deleting a Program Step ........................................................ 30
4.3.3
Converting a Gradient Step .................................................... 30
4.4 Deleting Programs ................................................................................. 31
5
PCR Run / Operation............................................................................ 32
5.1 User Login ............................................................................................. 32
5.1.1
Guest Users ........................................................................... 32
5.1.2
Registered Users ................................................................... 32
5.2 Changing Users ..................................................................................... 32
5.3 Selecting and Running a PCR Program ................................................. 33
5.4 Viewing Script Temperature Profiles Prior to Run .................................. 33
5.5 Opening and Closing the Lid .................................................................. 33
5.6 Loading the Cycler ................................................................................. 34
5.7 Setting the Lid Pressure ......................................................................... 35
5.8 Program Execution ................................................................................ 35
5.8.1
Starting a Run ........................................................................ 35
5.8.2
Terminating a Run.................................................................. 35
5.9 Reports .................................................................................................. 37
5.9.1
Viewing Reports ..................................................................... 37
5.9.2
Deleting Reports .................................................................... 38
5.10 Switching Off the Instrument ................................................................. 39
6
USB Memory Stick Operation ............................................................. 40
6.1.1
7
Importing and Exporting PCR Data Files ................................ 40
Maintenance ......................................................................................... 41
7.1 Cleaning................................................................................................. 41
8
Trouble-Shooting ................................................................................. 42
8.1 If a Power Failure Occurs ....................................................................... 42
8.2 Troubleshooting Information................................................................... 43
9
IV
Block Exchange ................................................................................... 45
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Removing the Block ................................................................................45
Inserting the Block ..................................................................................45
Appendix...........................................................................................................46
Instrument Specifications ...............................................................................46
Thermal Blocks .......................................................................................46
High Pressure Heated Lid .......................................................................46
Ambient temperature ..............................................................................46
10°C to 30°C...........................................................................................46
User Interfaces .......................................................................................47
User & File Management ........................................................................47
Reports and Validation............................................................................47
Networking & Barcode Option .................................................................48
Thermal Engine Characteristics ..............................................................48
Power & Dimensions...............................................................................48
Compatible Barcodes..............................................................................49
Compatible Plate Formats.......................................................................50
Order Information ..........................................................................................51
Sales & Service .............................................................................................52
Index.
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V
General Information
Policy Statement
It is the policy of Quanta Biotech to improve products as new techniques and
components become available. Quanta Biotech reserves the right to change
specifications at any time.
Warranty Statement
Quanta Biotech guarantees that the thermal cycler you have received has
been thoroughly tested and meets its published specification.
This guarantee is valid for 24 months only if the product and functions have
been used according to the instruction manual. No liability is accepted for loss
or damage arising from the incorrect use of the thermal cycler.
Quanta
Biotech's liability is limited to the repair or replacement of the unit or refund of
the purchase price at Quanta Biotech’s option. Quanta Biotech is not liable for
any consequential damages.
Quanta Biotech's thermal cyclers are for research use only.
Read the Instruction Manual carefully before using the thermal cycler to
ensure that you obtain the best possible results from the machine.
Quanta Biotech thermal cyclers should only be used by suitably qualified and
trained people. If the thermal cycler is not used as specified in this Manual,
the protection provided by the equipment may be impaired.
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1 Safety Warnings and Precautions
1.1
What you should know
Before
Operating the
Instrument
Ensure that anyone involved
with the operation of the
instrument is instructed in both
general safety practices for
laboratories and specific safety
practices for the instrument.
Always place the instrument in
a location where, if necessary,
the main power supply can be
disconnected immediately.
1.1.1 Instrument Safety
Please read this section before operating the thermal cycler. Operators of this
instrument must be trained in both general laboratory safety practices and the
specific safety requirements of the thermal cycler. If the equipment is used in
a manner not specified by the manufacturer, the protection provided by the
equipment may be impaired.
All functions performed within the context of preparing, performing and
completing a run should be done with caution and care, and with general
respect both to the instrumentation and to associated chemicals, samples and
other devices.
1.1.2 Symbols and Conventions
The following chart is an illustrated glossary of the symbols that are used in
this manual.
CAUTION This symbol indicates a potential risk and alerts you to
proceed with caution
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CAUTION This symbol indicates the presence of high voltage and
warns the user to proceed with caution
CAUTION This symbol indicates risks associated with hot surfaces
1.1.3 Potential Safety Hazard
1.1.3.1 ELECTRICAL
Standard electrical safety precautions should be applied.
1
Ensure that the proper voltage is supplied before turning the
instrument on for the first time.
2
The device must be connected to a grounded socket.
3
Do not touch any switches or outlets with wet hands.
4
Switch the instrument off before disconnecting the
AC power cord.
5
Unplug the instrument prior to cleaning up any major liquid spills
and prior to servicing any of the electrical or internal components.
Only qualified personnel should perform electrical servicing.
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1.1.3.2 EXPLOSIVE SUBSTANCES
Explosive, flammable and reactive substances should never be
cycled or incubated in any The thermal cycler.
Do not operate the The thermal cycler in a hazardous or potentially
explosive environment.
You must observe the relevant safety regulations when handling
pathogenic material, radioactive substances or other substances
hazardous to health.
1.1.3.3 PINCHING HAZARD
The lid presents a potential pinching hazard. Do not place hands or fingers
into the cycler when the lid is being opened or closed.
1.1.3.4 FLUIDS
Reaction vessels should be filled outside the cycler so that no fluids
penetrate the instrument.
Do not submerge the instrument in any liquid.
1.1.3.5 DANGER OF BURNS
Thermal block, inner side of heated lid and reaction vessels quickly
attain temperatures of greater than 50°C. Do not touch – Risk of
Burns! Keep the heated lid closed until temperatures of 30°C or
lower are reached.
Do not use any materials (plates, sealings, foils, mats) which are not
sufficiently temperature-stable (up to 120 °C).
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1.1.3.6 OPERATING ENVIRONMENT
Please ensure that the ventilation slots of the device remain free to
vent at all times. A space of at least 10cm should be left around the
thermal cycler
The ambient temperature should be between 10°C and 30°C, the
humidity between 0% and 95%.
WARNING:
All repair work must be carried out by Quanta Biotech
service engineers!
Only original Quanta Biotech replacement parts must be
used.
Disconnect the instrument from the mains supply before
opening.
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2 Installation
2.1
Requirements
The following steps must be followed for optimal and safe operation of the
thermal cycler:
2.1.1 Site
 The thermal cycler should be placed on a rigid, flat, clean surface.
Make sure that the instrument stands completely stable.
 Adequate ventilation is important. Make sure there is sufficient
space so that the rear and side air slots are not covered and to
allow cooling air to circulate freely around the instrument.
 There should be no paper under the device as this may block the
ventilation path. The unit should always have at least 10cm
distance to the next wall or neighbouring instrument.
 Quanta Biotech thermal cycler instruments were developed for
operation in laboratories in which there is normal ambient
temperature
and
no
explosive
atmosphere.
The
ambient
temperature should be between 10°C and 30°C the humidity
between 0% and 95%.
2.1.2 Electrical
 The thermal cycler must be connected to a grounded socket.
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2.1.3 Thermal Block
 Reaction vessels should be filled outside the thermal block so that no
fluids penetrate the instrument.
 To prevent damage to the block and the heated lid make sure you use
only recommended sample tubes. Unsuitable tubes can be damaged
causing the escape of sample material. This may present a serious
health hazard, especially when working with infectious materials.
 Make sure the PCR plates are positioned correctly in the block to
prevent damage to the block and the lid.
 Ensure that the heated lid is closed when heating tubes in the block.
Otherwise tubes may burst due to high temperatures thus causing the
escape of sample material.
 The lid presents a potential pinching hazard. Do not place hands or
fingers into the cycler when the lid closes otherwise hands or fingers
may be caught between the lid and housing.
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2.2
Unpacking the Thermal Cycler
2.2.1 Package Contents
The following parts are included in delivery:
Q-Cycler II
Server
Thermal Cycler
X
Power Cable 110
X
or 220V
Quanta Biotech
X
Thermal Cycler
User Manual on
CD or memory
stick
2.2.2 Initial Inspection
All parts of the instrument were thoroughly inspected and tested before the
unit was shipped, therefore the instrument should be in good operating order.
Carefully inspect the instrument and its accessories for any physical damage
sustained in transit.
If the instrument is received in a damaged condition, please file a claim with
the transport carrier immediately and contact Quanta Biotech for advice.
If the inspection has passed the Q-Cycler II thermal cycler is ready for use.
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2.3
Functional Overview
Quanta Biotech thermal cyclers are automated instruments designed for
carrying out the Polymerase Chain Reaction (PCR) and related methods for
amplification of DNA templates.
Figure 2-1: the Q-Cycler II (front view)
The Q-Cycler II has a manually operated lid with the ability to adjust pressure
for tubes and microplates.
The Q-Cycler II server is operated via a universal software application offering
independent control of the server’s own thermal block and any combination of
up to three, Q-Cycler II, S-96 or S-24 satellite thermal cyclers.
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2.3.1 Networking the Thermal Cycler
A network of up to 4 thermal cyclers consisting of one Q-Cycler II server and
up to three S-96 and S-24 satellite thermal cyclers can be established or a PC
network of up to fifteen S-96
and S-24 satellite thermal cyclers can be
established .
USB ports are provided at the side/back of the instruments to connect satellite
units to a Q-Cycler II server or a PC via standard USB instrument cables and
hub. Optional input devices such as barcode reader, keyboard, mouse or
printer can be connected in the same way (see USB connection diagram in
Figure 2-4 below).
2.3.1.1 Connecting S-96 and S-24 Satellite Thermal
Cyclers to the Q-Cycler II Server or PC.
1
Plug the thermal cycler’s AC power cord into the mains power connector
at the rear of the instrument and connect the other side of the cable with a
grounded AC outlet.
2
Plug the USB machine cable into the USB port at the side/rear of the QCycler II, S-96 or S-24 satellite thermal cycler.
3
Connect the other end of the USB cable directly to a Q-Cycler II server/PC
OR to a USB hub, if installing several devices.
4
Plug the cable connector of the USB hub into the USB port of the Q-Cycler
II server or PC
5
Boot up the Q-Cyler Server or PC
6
Press the power switch on the instrument(s).
7
Launch the application either by powering up the Q-Cycler II server or
launching the PCQB application.
8
The application will search for Q-Cycler II, S-96 or S-24 satellite thermal
cyclers that have been connected and display them in the cycle monitor
section of the application. The process can take up to two minutes
depending on the number of USB ports in use.
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Once found you can configure the thermal cyclers via the Settings menu in
the navigation bar. For details on software configuration of additional cyclers
refer to section Adding a Cycler on page 19.
2.3.1.2 Connecting Optional Devices
Optional input devices such as keyboard, mouse, printer and barcode reader
can be connected to the thermal cycler systems via a USB connection.
1
Plug the USB cable into the USB port of the input device.
2
Connect the other end of the USB cable to:
•
one of the USB ports of the thermal cycler/PC server, OR
•
a USB hub, if you wish to connect several input devices to a QCycler II/PC
3
When connecting several devices plug the cable connector of the USB
hub into the USB port of the thermal cycler server/PC.
4
For further configuration of the device consult the manufacturer’s
instruction manual.
2.4
AC Power Connection
2.4.1 Operating Power Requirements
All Quanta biotech thermal cycler models have self-regulating power. See
Instrument Specifications, Power & Dimensions on page 56 for power
specifications.
2.4.2 Fuses
WARNING:
Disconnect the AC power cord before
removing or installing a fuse to avoid the possibility of
serious injury from electrical shock!
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Fuse compartments are located on the rear of the instrument, above the main
power connection. Check the voltage rating label to verify that the instrument
is compatible with the AC voltage available at the installation site.
2.4.3 Grounding Requirements
DANGER: please read carefully!
To protect operating personnel, the National Electrical Manufacturers;
Association (NEMA) recommends that the instrument be correctly grounded.
The instrument is equipped with a 3 conductor AC power cord that, when
connected to an appropriate AC power outlet, grounds the instrument. To
preserve this protection feature, do not operate the instrument from an AC
power outlet that has no ground connection.
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3 Software Operation
3.1
Required Computer Skills
Users should have basic computing knowledge including using the mouse,
selecting items, and drag and drop.
3.2
Start Screen
Shortly after power up of the system, the screen first displays a start-up
routine. Then, the Start Screen appears.
Figure 3-1: Start screen with navigation buttons
Each button describes a task that can be performed upon clicking on it:
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New
Creates a New program.
Load/Edit
Opens the Explorer to browse stored PCR programs. Use this
Files
button to edit or load programs.
Wizard
Opens the Program Wizard dialog window where you can define
specific PCR information and a program will be automatically
generated
Start
Starts the loaded program. You can view the name of the
currently loaded program under the Q logo in the centre of the
screen
Incubate
Starts a single temperature incubation for predefined periods or
for an indefinite hold.
Login
Enables individual users to login and access there own files and
reports
Reports
Opens the report browser where you can search for stored GLP
and Lab reports.
Settings
Opens the User Settings dialog window where you can configure
user settings such as defining the default start screen and
manage cycler or user data, including power fail and OQ tests.
Q-Cycler II thermal cycler:
The logo in the centre of the screen serves as a retun to cycler run button.
Clicking on it connects you to the Q-Cycler II run screen for the cycler
selected.
The left and right arrows either side of the logo toggle between the server and
the different satellites connected.
If the server or satellite name is selected below the Q logo a summary run
screen showing all connected blocks appears.
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Selecting back takes you to the home screen.
3.3
Administrator Settings
Specific user settings can be defined in the Settings menu . Use the Settings
menu to add new thermal cycler devices or edit existing units, set time and
date, or manage user data such as setting up new users, and editing or
deleting existing accounts.
3.3.1 Cycler Management
Q-Cycler II software is designed for use with up to 4 thermal cyclers. Before
you can set up additional cyclers in the software, make sure that the new
thermal unit is connected to a Q-Cycler II
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1
To add a cycler simply connect the new cycler to the Q-Cycler II, power
up both units and launch the application. The new cycler will be
automatically recognized and installed ready to run. The name and details
of the newly connected unit can be changed by an administrator via the
settings tab.
3.3.1.1 Editing Cycler Data
1
The name and details of the newly connected unit can be changed by an
administrator via the settings button
2
Click on Cycler Management
3
Click on the thermal cycler you wish to edit and make the changes
4
Confirm your changes by clicking OK.
3.3.1.2 Setting Time and Date
1
To set the instrument time and date select Settings. Then select Cycler
Settings.
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2
Enter the system time and date by pressing the up and down arrow keys.
3
Confirm your changes by clicking OK.
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3.3.2 User
Management
3.3.2.1 User Levels
With the thermal cycler three different user levels can be distinguished:
Administrator
Registered User
Guest User
Each level has specific user rights. Only administrators are authorized to set
up new users and to change and/or assign user rights and passwords.
By default, the thermal cycler starts with Guest user rights.
User Rights
Functions allowed
Guest User
• Access rights to the Guest folder
• Creating, copying, editing and executing programs
stored in the Guest folder
Registered User
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• Access rights to the Guest and personal user
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folders
• Creating, copying, editing and executing programs
stored in the Guest and personal folders
Administrator
• Access rights to all Guest and user folders
• Setup and maintenance of users
• Setup and maintenance of cyclers
• Archiving/maintenance of all reports
3.3.2.2 First Start-up
Only the administrator is authorized to set up new users and to change or
assign user rights and passwords.
As a first-time user you will be logged in as "Guest” user visible in the top
status bar. Guest users usually have only restricted user rights and work from
the unprotected Guest folder, which can be accessed by all users.
Before you can operate the thermal cycler as a regular user, your
administrator has to set you up as a new user. Once you are a registered
user, you have a personal, encrypted file folder where you can administer
your own PCR program files.
3.3.2.3 Logging in as Administrator
After starting the thermal cycler, the Start screen appears displaying the
thermal cycler’s navigation elements.
1
Click on the Login button in the top status bar.
2
Select Administrator from the Username drop-down list and enter the
administrator password. The default administrator password is admin
1
Click Login to confirm your settings.
2
You are now logged in as Administrator (visible in the top status bar).
IMPORTANT:
It is recommended that the factory-set login
password be changed to prevent unauthorized access to the
instrument
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3.3.2.4 Adding or Modifying User Accounts
NOTE: Only the administrator can set up new users or modify existing user
accounts.
Adding New User Accounts
1
To add a new user, click Settings. The User Settings dialog opens.
3
In the User Management section click OK.
The User Management dialog opens listing the available user names and
corresponding user levels.
4
Click New User to open the Add a new User dialog.
5
Assign a user name and password..
6
Select the required user level (Guest, User, Administrator) from the dropdown list.
7
Now you can define the user’s script reading and writing privileges by
checking the appropriate boxes:
8
Edit/Rename Script
The user has the right to edit or rename a
program file
Delete Script
The user has the right to delete program files
Create Script
The user has the right to create program files
Once you have entered all data, click on Create User to save the settings.
After successful setup, a confirmation message will be displayed and the
new user name will be added in the User Management list.
Modifying User Accounts
1
To modify an existing user account click Settings. The User Settings
dialog opens.
9
In the User Management section click OK.
The User Management dialog opens listing the available user names and
corresponding user levels.
10 Click Edit User to open the Edit a User dialog.
2
Here you can modify user data such as name, user level, password, user
privileges and script directory protection.
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3
Click OK to save the changes.
A confirmation message will be displayed after successful update of the
user data.
Deleting User Accounts
NOTE: User accounts can only be deleted by the administrator.
1
To delete an existing user account click Settings. The User Settings
dialog opens.
2
In the User Management section click OK.
The User Management dialog opens listing the available user names and
corresponding user levels.
3
Select the user name you wish to remove.
4
Click on Remove User to remove the user name from the user list. It
removes all user permissions that the user has and does not allow that
user to access any the thermal cycler device. It also deletes all scripts and
GLP reports associated with that user.
NOTE: The Remove User command also deletes all scripts and GLP reports
associated with that user. The Administrator user account cannot be deleted.
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4 Programming
4.1
Creating PCR Programs
4.1.1 DND Programming
Drop-and-Drag programming (DND programming) is an easy and intuitive
method for conveniently creating PCR programs step-by-step.
The basic idea of DND programming is that you simply choose the required
command from a command list, drag it with the mouse into the program
window on the left and drop it into the desired program position. This opens a
dialog window where you can define further commend-specific settings such
as time, temperature, lid pressure, etc. In this way you can create your PCR
program command by command according to the planned programming
sequence.
The command order can be changed easily any time just by dragging the
selected command on the desired position within the program window.
Once the program is started the cycler will process the commands from top to
bottom according to the defined programming sequence.
1
To generate a new PCR program using DND, just click
on the
Start screen. This will open the Compose a New PCR Program dialog
with its drag-and-drop functions:
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Figure 4-1: Compose a New PCR Program dialog
The dialog window consists of the Command List, which contains all available
program steps, and the Program Window where you can create or edit PCR
programs.
11 Select a command from the Command List, then drag and drop it into the
Program Window. This will open the dialog window of the selected
command.
12 Enter your settings in the dialog fields of the selected command.
13 Confirm your settings by clicking OK. This will return you to the Compose
a New PCR Program window.
14 To add another command to your program repeat steps 2–4 for each
required command.
15 Once you have finished creating your program, save it by clicking on Save
As and you will be prompted for a file name.
16 Name your file whatever you like within Windows naming conventions.
17 Click on Profile, if you wish to view a graphical representation of the
defined temperature profile.
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4.1.1.1 Program Steps
The following program steps are available in the Command List for creating
customized PCR programs in the DND environment:
Barcode Input
Use this command to define barcode settings. When composing the program,
it is recommended to place the barcode command at the beginning. During
program run you will be asked to read the plate's barcode. You may do this
with a handheld USB barcode scanner or by manually entering the barcode. If
you use the barcode scanner, the barcode cannot be edited after reading.
The program will continue after you have placed the plate into the cycler and
pressed OK. The barcode will be recorded in the GLP and LabBook report
and displayed in the Info section of the Cycler screen.
Scan Using Barcode Entry: Barcode reading using the barcode scanner
Scan Using Keyboard Entry: Manual entry of the barcode via keyboard
Path for worklist: Specifies the path for the worklist.
Automatic Hot Start
Automatic Hot Start takes advantage of novel Polymerase technology, which
enables the performance of an extended hot start without degrading the
enzyme at high temperature. This is realized e.g. using a bead or antibody
protection. The protection is released after the extended denaturation is
elapsed and the enzyme starts working.
When using the Automatic Hot Start, you just have to select the denaturation
temperature and time. Running the program, the next command will be
executed immediately after the denaturation time is elapsed.
Denaturation Temperature: Specified denaturation temperature
Hold for: Specified time of the program step
Use with automatic Hot Start Polymerase: Examples for denaturation times
Confirmation Change Polymerase 900 sec denaturation
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Antibody protected Polymerases 360 sec denaturation
Please also refer to the polymerase manufacturer’s recommendations
Manual Hot Start
When selecting the Manual Hot Start command, you just have to supply the
denaturation temperature and time. The instrument will notify you after the
denaturation time is elapsed. You will then be asked to open the cycler and
add the enzyme. The instrument timer is now on hold and will continue only
after you have pressed OK in the displayed Hot Start information window.
Denaturation Temperature: Defined denaturation temperature
Hold for: Specified time of the program step
Lid
Use this command to set the required lid temperature.
Lid Temperature:
To determine the temperature of the heated lid. The
temperature may range from 100°C to 112°C. The
minimum increment is 1°C.
Lid is ON:
Select this option to activate lid heating.
Temperature Step
Use this command to increment the processing temperature (in °C) by a
preset value and to define the step duration.
Processing Temperature: Specified processing temperature in °C.
Hold for: Specified time of the temperature step.
Temperature Ramp: Rate of change of the sample temperature. Rates can be
from 0.1°C to 3°C per second. When setting the ramp to zero, the instrument
ramps with maximum capacity.
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Temperature Increment: Temperature increment and decrement per cycle.
Steps can be from 0.1 °C to 10 °C.
NOTE: For decrement use negative figures (e.g. -2)!
Time Increment: Time increment per cycle in seconds.
Step Type: Indicates the selected PCR step type for information purposes.
The supplied step type information will then be displayed in the online cycler
monitor.
Touchdown
Touchdown is an advanced PCR technique used to reduce unspecific binding.
With the thermal cycler, Touchdown PCR is provided as a one-step command
for easy implementation in the programming sequence.
Denaturation Temperature: Defined denaturation temperature.
Maximum Annealing Temperature: Sets the maximum annealing temperature.
Minimum Annealing Temperature: Sets the minimum annealing temperature.
Hint: The temperature difference is commonly between 5°C and 10°C starting
with a temperature that is 2°C above the higher Primer melting temperature.
Example: if Tm Primer 1 is 60°C and Tm Primer 2 is 54°C, the resulting
Touchdown will be from 62°C to 52°C.
Elongation Temperature: Sets the elongation temperature.
Cycles: Number of required cycles for the Touchdown section.
Duration: Duration of the temperature step in seconds.
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Gradient Step
Use this command to define settings for the gradient function. Just enter the
required minimum and maximum temperature gradient and time.
Minimum Temperature Gradient: To set the minimum temperature of the
gradient step. Min. temperature must not be below 30°C.
Maximum Temperature Gradient: To set the maximum temperature of the
gradient step. Max. temperature must not exceed 80°C.
NOTE: The maximum / minimum gradient span is 20°C / 4°C.
Hold for: Duration of the Gradient step.
Convert Gradient Step to a standard temperature step: The Gradient step can
be converted easily to a temperature step after the annealing temperature
optimization experiment. To do so, just check the checkbox and select the
temperature column with the best result of your optimization experiment. The
converted gradient step will then automatically use the temperature of this
best-performing column.
Gradient steps are not available for the S-24 thermal cycler
NOTE: Resulting temperature steps may be reconverted to
gradient steps any time by disabling the Conversion option
Start Cycle
Starts a PCR cycle.
Cycle Name: Enter the name of the cycle.
Number of Loops: Enter the required number of loops.
End Cycle
Insert this command to terminate a PCR cycle.
Store
26
ExpressGene
Cools the thermal cycler down to a temperature between 4°-12°C for a
specified period of time.
Store Temperature: Storage temperature in °C.
Hold for: Duration of the Store step.
Infinite: Holds the specified temperature for an unlimited period of time.
NOTE: When executing the Store command, the lid pressure is
set to zero Newton
i
Script Information
Use this command to record your comments about the PCR program.
4.1.2 Program Wizard
The Program WizardTM is a unique feature that makes it easy for first-time
PCR users to write their own protocol. The Program Wizard takes care of the
calculations required for generating a PCR program. All you need is to supply
the biological data of the Primer and template DNA such as Primer sequence
or melting temperature, template length and origin of DNA.
The Program Wizard is also an ideal tool for first shot PCRs as it uses proven
calculations, e.g. for the Annealing Temperature.
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27
1
To generate a new PCR program using biological data, click on Program
Wizard in the start screen.
2
The Primer Information dialog opens from where you will be guided step
by step through the programming procedure.
3
After you have supplied all required data click on Generate PCR Program.
The Compose a New PCR Program dialog window opens where you can
view and edit the previously defined program steps.
4
To edit a program step, double-click the respective command.
5
To view a graphical representation of the defined temperature profile, click
Profile.
4.2
General Programming Guidelines
To ensure a correct PCR run, the following guidelines should be followed
when composing a new program:
28
•
A program must contain at least one Temperature Step.
•
Cycles cannot be nested. A started cycle must have an End Cycle
ExpressGene
command before starting the next Start Cycle command.
•
Barcode Input command cannot be programmed inside a cycle.
•
Only a single Script Information command is valid within a
program.
The following commands or command combinations are considered "stages":
Automatic Hot Start
Manual Hot Start
A string of up to ten single Temperature steps
Single Gradient steps
A complete cycle including Temperature or Gradient steps
within a start cycle / end cycle pair.
A Touchdown command
A Store command
When using these stages in a script, the following rules apply:
•
The maximum number of stages within a script must not
exceed 10.
•
The maximum number of Temperature and Gradient commands
inside a cycle must not exceed 10.
•
4.3
The maximum number of cycles/loops within a stage is 99.
Editing Programs
4.3.1 Editing Program Parameters
You can edit the parameters of existing programs and store the program with
the same name or rename the edited program.
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29
To edit an existing PCR program click on the load/edit button on the main
screen:
1
Depending on your user level (guest or registered user), the specific user
directory or guest directory is displayed listing all existing PCR program
files.
2
Select the program you wish to edit.
3
Click Open.
Opening the file automatically loads the PCR program and the program
steps are displayed in the Program Window of the Compose a new PCR
Program dialog.
4
Double-click the command you wish to edit. This will open the dialog
window of the respective command.
5
Edit the parameters as needed.
6
Click Save to save your changes and to return to the Compose a new
PCR Program window.
7
Repeat steps 4–6 for each command you wish to edit.
8
After you have modified all data, click Save in the Compose a new PCR
Program dialog, if you wish to store your changes in the existing PCR
program. OR
9
Click Save As, if you wish to rename the program. You will then be
prompted for a file name.
4.3.2 Deleting a Program Step
1
To remove a program step from the program just drag it from the Program
Window and drop it into the recycle bin
icon located below the
Program Window.
2
Save the program as described above.
4.3.3 Converting a Gradient Step
A gradient step can be converted to a standard temperature step with a few
mouse clicks.
30
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1
After performing the gradient experiment, have a look at the gel image and
select the best-performing column.
2
Double-click the Gradient Step command to open its dialog window.
3
Select the Convert Gradient Step to a standard temperature step
checkbox.
4
Select the appropriate column from the drop-down list in the Conversion
section.
the thermal cycler software will now convert the gradient step into a
temperature step and automatically select the right temperature in the
chosen column.
NOTE: If it is required to repeat the gradient experiment, the converted
temperature step can be set back to a gradient command just by unchecking
the Conversion option
4.4
Deleting Programs
1
To delete an existing PCR program Click Load Edit in the start screen.
2
Depending on your user level (guest or registered user), the specific user
directory or guest directory is displayed listing all existing PCR program
files.
3
Select the program you wish to remove.
4
Click Delete. This will remove the program from the script folder.
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31
5 PCR Run / Operation
5.1
User Login
5.1.1 Guest Users
When powering up the instrument the default user level is Guest. If you wish
to continue to operate the cycler with Guest user rights, no special login
procedure is required. In this case you can access the unrestricted Guest
folder and operate the cycler as described in the relevant chapters.
5.1.2 Registered Users
Once the administrator has set you up as a registered user (see 3.3.2.4), you
can operate the thermal cycler using your personal data and file folders.
To log into the system as a registered user proceed as follows:
1
Click on the Login button in the main screen– the Login dialog opens.
2
Select your user name from the Username drop-down list and enter your
password.
3
Click on the Login button. This will log you into the system giving you
reading and writing rights as specified for your user level.
4
After successful login your user name is displayed in the top status bar.
NOTE: In case a user forgets his or her password, the logged-on administrator
can assign him/her a new one.
5.2
Changing Users
In order to log into the system when another user is already logged in,
proceed as described in section Registered Users above.
32
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5.3
•
1
Selecting and Running a PCR Program
To select an existing PCR program choose Start from the main screen
Your personal file folder opens or, if you are logged in as a guest, the
guest folder opens displaying the available PCR programs.
2
Select the program you wish to run.
3
Click Run selected on the right-hand side of the window to start the
selected program.
NOTE: The Start PCR command button in the navigation bar on the left-hand
side of the screen will start the currently loaded program, e.g. the previously
used program, but not the selected program!
4
If you wish to view the PCR program steps, click Open.
Opening the file automatically loads the PCR program. The name of the
loaded program is displayed in the top status bar.
5
After a program is loaded you may view its script parameters prior to run
by clicking on Profile.
5.4
Viewing Script Temperature Profiles Prior to Run
Before starting a run, you can view the temperature profile of a new or stored
program.
1
If not yet displayed, open the desired program as described in section 5.3,
Selecting and Running a PCR Program.
2
Click on Profile in the Compose a New PCR Program dialog window.
An information screen is displayed detailing all the parameters of the
selected program.
5.5
Opening and Closing the Lid
For Q-Cycler II thermal cyclers the lid can be opened and closed manually at
any stage.
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33
5.6
Loading the Cycler
For Q-Cycler II thermal cyclers open the lid manually.
WARNING:
Danger of Burns! The thermal block, test tubes and
plates may reach temperatures as high as 100°C! Before
loading or unloading the cycler, keep hands away until
temperature reaches 30°C or less
Pinching Hazard! The lid presents a potential pinching
hazard
D o not place hands or fingers in to the cycler when the lid
closes
Loading Plates
Depending upon the thermal block installed, the thermal cycler can be loaded
with one 96-well PCR plate or one 384-well PCR plate.
Put your plate on the block and ensure that it is correctly positioned in the
provided space.
Loading Tubes
Q-Cycler II thermal cyclers can be loaded with up to 96 PCR tubes (0.2 ml) or
48 PCR tubes (0.5 ml) when using the 96-well combi block.
Distribute tubes equally over the block.
NOTE: When using a Gradient step inside your program, use the central rows in
the block (rows D + E).
34
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5.7
Setting the Lid Pressure
Simply close the lid and turn the knob. This sets the pressure to low pressure
for tubes. If high pressure for plates is required simply turn the knob slightly
further
5.8
Program Execution
5.8.1 Starting a Run
To start a run click start from the main screen
Your personal file folder opens or, if you are logged in as a guest, the guest
folder opens displaying all available PCR programs.
1
Select the program you wish to run.
2
Click Run selected on the right-hand side of the window to start the
selected program.
NOTE: The Start PCR command button in the navigation bar on the left-hand
side of the screen will start the currently loaded program, e.g. the previously
used program, but not the selected program!
After a run is started, a graphical representation of the temperature
parameters is displayed. In addition, detailed program and instrument
information can be viewed using the Program or Info buttons located in the
right-upper corner of the information screen.
Click Program to display the program steps of the running protocol. The
executed step is highlighted.
Click Info to view detailed instrument information such as temperature sensor
values, lid status, remaining time and step time or GLP file name.
5.8.2 Terminating a Run
After a run is started, the Start PCR button in the start screen change to Stop
PCR.
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35
1
To stop a program manually before it completes, ensure the appropriate
thermal cycler is selected and press Stop PCR.
To open the cycler turn the knob located on the top of the instrument
The manual abortion of the program will be recorded in the GLP and lab book
reports.
36
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5.9
Reports
5.9.1 Viewing Reports
Depending on the assigned user level, you are allowed to view and print
LabBook reports stored in the following file folders:
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37
User rights
File folder
Action
Guest users
Guest folder
Viewing and printing Lab Book reports
The access to GLP reports is denied.
Registered users
Guest folder &
Viewing and printing Lab Book reports
Personal file
The access to GLP reports is denied
folder
Administrator
All guest and
user folders
Viewing Lab Book reports
Access to GLP reports permitted
To view reports proceed as follows:
1
Click Reports in the navigation bar. This will open the Browse Generated
Reports window listing the generated reports.
2
Group the displayed reports by selecting one of the sorting criteria below:
Group by Date:
Sorts records in ascending order by date.
Group by Cycler:
Sorts records by cycler name.
Group by Program:
Sorts records by program name.
3
For viewing GLP and/or LabBook reports select one of the following
options:
Show GLP:
Displays the generated GLP records (only for users
with administrator rights)
Show LabBooks:
Displays the generated LabBooks
5.9.2 Deleting Reports
To delete reports proceed as follows:
1
Open the Reports dialog window as described above.
2
Select the report you wish to remove.
3
Click Delete to remove the report from the reports list.
NOTE: Only users with administrator rights are authorized to delete reports.
38
ExpressGene
5.10 Switching Off the Instrument
The unit can be switched off at any time during operation using the power
button at the front of the instrument. There is no need to exit the thermal
cycler software prior to switching off the instrument.
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39
6 USB Memory Stick Operation
6.1.1 Importing and Exporting PCR Data Files
You can import Primer sequences and PCR programs created on a different
thermal cycler or on a PC, or export PCR programs and reports that have
been created using a USB memory stick.
Exporting Program Files to a USB Archive
1
Make sure the USB memory stick is inserted in the USB slot on the front
of the instrument.
2
Click View & Edit Files to display your personal file folder. Select the
desired PCR program as described in section 5.4 Selecting a PCR
program.
3
Click Save As to operate the Save Program dialog window.
4
Enter the name of the program.
5
Activate the Save to USB checkbox. The file is then saved to the USB
archive.
NOTE: the lock button on the memory stick may prevent data access. Please
ensure the lock button is in the unlocked position.
Importing Files from a USB Archive to the Thermal Cycler
40
1
Make sure the USB memory stick is inserted in the USB slot on the front of
the instrument.
2
Click View & Edit Files to display your personal file folder. Click View
USB to access the USB archive.
3
Select the desired file from the archive.
4
Click Save As to select a file name and path.
5
Confirm with OK to save the file.
ExpressGene
7 Maintenance
7.1
Cleaning
Quanta Biotech thermal cyclers are designed to require a minimum amount of
maintenance by the user. They can be cleaned using water or a mild
laboratory-cleaning agent.
The instrument should not come into contact with organic solvents or
aggressive solutions. Ensure that no liquid enters the thermal cycler. For
safety reasons, the device must be switched off and disconnected from the
power supply prior to cleaning.
The electrical safety fuses are located above the main power switch and the
main power plug at the rear of the device. Before the fuses are replaced, the
device must be switched off and disconnected from the mains supply. Only
fuses with the correct voltage values may be used.
Only qualified service personnel should perform servicing of the thermal
cycler.
No warranty obligation is assumed by Quanta Biotech in the event of damage
caused by unauthorized servicing.
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41
8 Trouble-Shooting
8.1
If a Power Failure Occurs
All Quanta Biotech thermal cyclers are equipped with an autopower fail
restart function that allows for power failures and safe continuation of a PCR
run after resumption of power if set to do so in the cycler settings.
The autopower fail restart function restarts the script from the beginning of the
temperature step in which it failed.
The following table lists the three power failure scenarios and the actions the
instrument takes if the power is interrupted during operation.
Observation
Explanation / Remedy / Actions
The computer fails but the
thermal cycler unit still has
power
In this case the thermal engine will continue to
execute the script without any interruption.
The thermal cycler fails but
the computer still has power
The execution of the script will be affected and will
halt whilst the power is off. Once the power resumes,
restart the unit and the script will resume from the
beginning of the step it was executing when the power
failed.
Both the computer and the
thermal cycler unit lose
power
The execution of the script will be affected and will
halt whilst the power is off. Once the power resumes
the script will resume from the beginning of the step it
was executing when the power failed.
Corrective action:
Restart the application and the connection will be
restored.
42
Trouble-Shooting
Auto-Q
8.2
Troubleshooting Information
Refer to the following table for a description of potential problems and
recommended actions that you should take.
Observation
Explanation / Remedy / Actions
No actual temperature
displayed
Block not correctly inserted in the machine.
Corrective action: Check that the block is inserted
correctly and reboot the machine.
(1)
One thermal engine
thermistor does not agree
with the others
Run Thermal Engineer OQ test to assess whether
performance is within specified limits.
Poor experimental yield
1. Run Thermal Engine OQ test test to check that
thermal engine operation is within specification.
(1)
2. If transferring protocol from a different make of
(2)
thermal cycler, use TAS system to determine new
protocol parameters.
Program does not start
Block not correctly inserted in the machine.
Corrective action:
Check that the block is inserted correctly and reboot
the machine.
Tubes are crushed
Applied pressure too high.
Corrective action:
Reduce pressure applied using lid knob
Condensation in tubes during
a run
Condensation will appear naturally at the end of a run
and has no deleterious effects. Simply spin or flick
the liquid back to the bottom of the tube and continue
with the post PCR steps. However, condensation
during a run will result in sub-optimal cycling. If this is
experienced try increasing the lid temperature.
Sealed microplate losing
sample volume
Poor quality microplate/sealing system or inadequate
pressure.
Corrective action:
Increase the pressure by turning the knob in the lid
USB devices including
memory stock and barcode
scanner not recognised
Reboot machine.
Satellites are not recognized
by the application
Check all satellites are powered and connected.
reboot Server.
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43
Observation
Explanation / Remedy / Actions
Machine does not power
up
Check power is on at supply.
Check fuses at rear of instrument above power inlet.
Blank Display lit but
showing no information
Reboot machine. If the problem persists there is an
internal connection problem: call Technical Support.
Abnormal block
heating/cooling
performance
Run Thermal Engine OQ test
(1)
to check performance.
(1) Please refer to the thermal cycler IQ/OQ Protocol document for
instructions on performing instrument qualification tests.
(2) The Temperature Acquisition System (TAS) can be purchased separately
from Quanta Biotech Ltd.
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9 Block Exchange
Block exchange can be performed within seconds. No tools are required for
this purpose.
CAUTION:
Turn power off and interrupt power line before changing
the block
Removing the Block
Remove the block by simply holding it with two hands on the left and right side
and lift it up.
Figure 9-1: Exchanging the block
Inserting the Block
Hold the block with two hands on the left and right side, with the block
connector at the back of the block, and place it into position in the thermal
cycler.
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45
Appendix
Instrument Specifications
Thermal Blocks
Block Materials
Modular anodized aluminium or gold coated
silver blocks with 4 sensors
Traceability
NIST traceable temperature calibration
Blocks Available
-
96 well gradient block: For 96 well PCR
plates or 96x 0.2 ml tubes
-
96 well gradient combi block: For 96 well
PCR plates, 96 x 0.2 ml tubes
-
4 slide/microarray
-
384 well high throughput block: for 384
well plates
High Pressure Heated Lid
Lid Temperature Range
To 112°C, user selectable in 1°C
increments
Lid Pressure Range
Lid pressure manual - Compatible
with all seals
Ambient temperature
Relative Humidity
46
10°C to 30°C
0% to 95%
ExpressGene
User Interfaces
Screen Type
6.4 inch TFT colour touch-screen
System Input
Touchscreen, external keyboard &
mouse (USB), barcode scanner (USB)
Temperature Display
Real-time graphical display of actual &
set block temperatures
Real-time digital display of all sensor
values
Communication Interfaces
1x USB front (personal memory stick)
User & File Management
User Level Management
3 levels: Administrator, User with
administrator
selectable restricted
rights, Guest
User File Protection
Yes, for programs and reports
File Organization
Windows® Explorer, user-defined
folders & subfolders
Program Storage
Minimum 1000 (internal memory or
USB stick). Unlimited PC program
storage
Reports and Validation
Report Function
Encrypted GLP report, LabBook report
Validation
Internal auto validation prior to each
program start
Thermal engine validation function
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47
Networking & Barcode Option
Networking
Barcode Option
Cycler network up to 4 units
Server - Satellite system network up
to 4 units
Barcode documentation via
handheld barcode scanner
Thermal Engine Characteristics
Temperature Control
4°C - 99.9°C with simulated volume
dependent control algorithm
Sample Volume Range
5-100µl
Sample Accuracy
+/- 0.4°C (20-99°C), +/- 1°C (4-20°C)
Sample Homogeneity
+/- 0.4°C
Heating
Up to 5°C per second
Cooling
Up to 3°C per second
Sample Overshoot
< 1°C
Gradient Temperature Range
30°C - 80°C (96 well only)
Maximum/minimum Gradient Span
30°C / 4°C
Power & Dimensions
Electronic Power Supply
Dimensions
Weight
Operating conditions
Regulatory
Warranty
100V to 240V (frequency 48 to
62Hz)
(WxDxH) 34cm x 42cm x 26cm
12 kg approx
10°C - 30°C, 0 - 95% relative
humidity
CE, UL, CSA compliant
2 years on all systems
48
ExpressGene
Compatible Barcodes
The following barcodes are compatible with THE Q-Cycler II's documentation
system:
ExpressGene
•
2 of 5 interleaved
•
Codabar
•
Code 128
•
Code 39
•
Code 39 full ASCII
•
Code 93 full ASCII
•
EAN-13, EAN -13 with 2 supplement, EAN -13 with 5 supplement
•
EAN-8, EAN -8 with 2 supplement, EAN -8 with 5 supplement
•
EAN-128
•
UPA, UPA -13 with 2 supplement, UPA -13 with 5 supplement
•
UPE, UPE -13 with 2 supplement, UPE -13 with 5 supplement
•
MSI
•
C.I.P
49
Compatible Plate Formats
The following plate formats are compatible with the thermal cycler:
Manufacturer
Art. No. & Product Name
Type
AB 800
96 well, skirted
AB 900
96 well, semi-skirted
AB 1111
384 well
TF-0384
384 well
THERMOSPRINT® 96 PP, transparent,
311296; only w/o workstation
96 well, non-skirted
ABgene
Bilatec
THERMOSPRINT® 384 PP, transparent, 384 well
310384
NUNC
Art. No.: 240600, 96 well, non-skirted.
96
Art. No.: 230013, 96 well, semi-skirted
96
Art. No.: 230012, 96 well, skirted
96
Art. No.: 230584, 384 well
384
Eppendorf
Art. No.: 0030 128.648, twin tec PCR
plate, 96 skirted
Art. No.: 0030 128.575, twin tec PCR
plate, 96 semi-skirted
Art. No.: 0030 128.508, twin tec PCR
plate, 384
Corning
Thermowell®96 Well Polypropylene PCR
Microplate, Natural (#6551)
Thermowell™ 96 Well Polycarbonate
PCR Microplate, Model M (#6511)
Corning® 384 Well Polyproplyene PCR
Microplate (#6500)
Corning® 384 Well Polyethylene PCR
Microplate (#6502)
50
10BAppendix
ExpressGene
Order Information
Part #
Description
Thermal Cycler
7013001
Q-Cycler II Server Standard Thermal Cycler including block –
please specify standard block required
7013002
Q-Cycler II Server Gradient Thermal Cycler including block –
please specify gradient block required
7013003
Q-Cycler II Server Ultra Gradient Thermal Cycler including
block – please specify ultra block required
7013004
Q-Cycler II Satellite Standard Thermal Cycler including block –
please specify standard block required
7013005
Q-Cycler II Satellite Gradient Thermal Cycler including block –
please specify gradient block required
7013006
Q-Cycler II Satellite Ultra Gradient Thermal Cycler including
block – please specify ultra block required
Thermal Blocks
7004001
Standard Block for 96x0.2ml/96well plate
7004002
Standard Block 96x0.2/48x0.5ml
7004003
Block for 4 Slides/Microarrays
7004004
Gradient Block for 96x0.2ml/96well plate
7004005
Gradient Block 96x0.2/48x0.5ml
7004006
Gradient Block for 384 well plate
7004007
Ultra Gradient Block for 96x0.2ml/96well plate
7004008
Ultra Gradient Block for 384 well plate
Manual
QBTCMAN
ExpressGene
Quanta Biotech Thermal Cycler Manual
Operator manual for the thermal cycler and the thermal cycler
Software
51
Sales & Service
Quanta Biotech Ltd
Unit 4 Byfleet Technical Centre
Canada Road
Byfleet
Surrey
KT14 7JX
United Kingdom
Phone +44 (0) 1932 344550 • Fax +44 (0) 1932 353108
Email: [email protected]
Web: www.Quantabiotech.com
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52
Index
AC Power Connection, 11
Operating Power Requirements, 11
Adding New User Accounts, 19
Operation, 32
Adding or Modifying User Accounts, 19
Changing Users, 32
Administrator Login, 18
Opening and Closing the Lid (QB-24
Administrator Settings, 15
and QB96), 33
Automatic Hot Start command, 23
Running a Program, 35
Barcode Input command, 23
Sample Loading, 34
Block Exchange, 45
Selecting Programs, 33
Changing Users, 32
Setting the Lid Pressure, 35
Cleaning, 41
Terminating a Run, 35
Close Lid command, 24
User Login, 32
Connecting Auto-Q Satellite, QB-96 and
Viewing Script Temperature Profiles
QB-24 Thermal Cyclers to the Auto-Q
Server, 10
Prior to Run, 33
Order Information, 51
Connecting Optional Devices, 11
Package Contents, 8
Creating PCR Programs, 21
Program Execution, 35
Cycler Management, 15
Program Steps, 23
Deleting a Program Step, 30
Automatic Hot Start, 23
Deleting Programs, 31
Barcode Input, 23
DND Programming, 21
Close Lid, 24
Editing Cycler Data, 16
End Cycle, 26
Editing Programs, 29
Gradient Step, 26
Electrical, 6
Manual Hotstart, 24
End Cycle command, 26
Script Information, 27
First start-up, 18
Start Cycle, 26
Functional Overview, 9
Store, 26
Fuses, 11
Temperature Step, 24
Gradient Step command, 26
Touchdown, 25
Gradient Step Conversion, 30
Program Wizard, 27
Grounding Requirements., 12
Programming, 21
Initial Inspection, 8
Converting a Gradient Step, 30
Installation, 6
Deleting a Program Step, 30
Loading the Cycler, 34
Deleting Programs, 31
Maintenance, 41
Editing Programs, 29
Manual Hot Start command, 24
General Guidelines, 28
Modifying User Accounts, 19
Program Wizard, 27
Networking the Thermal Cycler, 10
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Reports, 37
53
Deleting Reports, 38
User & File Management, 47
Viewing Reports, 37
User Interfaces, 47
Requirements, 6
Start Cycle command, 26
Running a Program
Start Screen, 13
Starting a Run, 35
Safety
Store command, 26
Instrument ~, 2
Switching Off the Instrument, 39
Symbols and Conventions, 2
Symbols and Conventions, 2
Safety Warnings, 2
Temperature Step command, 24
Sales & Service Centers, 52
Terminating a Run, 35
Script Information command, 27
Thermal Block, 7
Setting Time and Date, 16
Touchdown command, 25
Site, 6
Trouble Shooting
Software Operation, 13
Required Computer Skills, 13
Specifications, 46
If a Power Failure Occurs, 42
Trouble-Shooting, 42
UnPacking the Thermal Cycler, 8
Compatible Barcodes, 49
USB Memory Stick Operation, 40
Compatible Plate Formats, 50
User Accounts
Neworking & Barcode Options, 48
54
Starting a Run, 35
Deleting Accounts, 20
Power & Dimensions, 48
User Levels, 17
Reports and Validation, 47
User Login, 32
Thermal Engine Characteristics, 48
User Management, 17
ExpressGene
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