AIAS 3000 Xcalibur Setup Rev D

AIAS 3000 Xcalibur Setup Rev D

TRACE GC

AI/AS 3000 Autosampler

User’s Manual

Published by Technical Publications, Thermo Finnigan Italia S.p.A

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20090 Rodano-Milan

Italy

Printing History: Revision D printed March 2002.

Xcalibur

and TRACE GC

are trademarks and/or product names of Finnigan Corporation. Microsoft

®

is a registered trademark of

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Contents

Chapter 1

AI/AS 3000 Set Up View ...............................................................................................................5

1.1 Configuration .................................................................................................................................6

Configuration Screen .............................................................................................6

1.2 AI/AS 3000 Method Editor ............................................................................................................7

AI/AS 3000 Page ...................................................................................................7

Sampling ................................................................................................................7

Injection .................................................................................................................8

Washes ...................................................................................................................8

AI/AS3000 Command Menu .................................................................................9

1.3 Setting AI/AS 3000 Parameters ...................................................................................................10

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Chapter 1

AI/AS 3000 Set Up View

This chapter contains informations to configure and to set the AI/AS 3000

Autosampler

This chapter contains these topics:

Configuration

AI/AS 3000 Method Editor

Setting AI/AS 3000 Parameters

6

7

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AI/AS 3000 Set Up View

Configuration__________________________________________________________________________________

1.1 Configuration

Configuration Screen

Items listed under this heading contain definitions to each control you see listed in this Instrument Configuration window.

Figure 1-1. AI/AS 3000 Configuration Screen

Connection

Serial Port

This parameter specifies the communication port to be used for the AI/AS

3000 sampler, it can be through the GC or alternatively can be any COM Port from 1 to 8.

Number of vials

8 Vials

Check this box if you are using the AI 3000 autosampler

105 Vials

Check this box if you are using the AS 3000 autosampler

Syringe Type

Volume

This parameter specifies the volume of the syringe in use.

Click OK after you are satisfied with your selection. Click Cancel if you would like to exit without saving your changes. Click Help for this page to display.

1-6

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AI/AS 3000 Set Up View

____________________________________________________________________ __ AI/AS 3000 Method Editor

1.2 AI/AS 3000 Method Editor

The Method Editor screen has four tabs which contain all of the parameters used to set up injection events. Click the links below to find out more about the parameters in the each tab.

AI/AS 3000 Page

Sampling

Sampling Group Box

This group box contains the parameters to control syringe rinse, bubble elimination, sample pull-up speed from the vial to the syringe and needle penetration depth into the sample vial.

Sample volume (ml)

This is the volume in microliters of sample injected..

With the 10 µL syringe set a value from 0 to 5 µL in step of 0.1 µL.

With the 5

µL syringe set a value from 0 to 2.5 µL in step of 0.1 µL.

Plunger strokers

The number of times the syringe plunger will be pulled up and down to eliminate any bubbles from the sample.

Set a value from 0 to 15.

Viscous sample

It calculates automatically the speed the sample is pulled-up

(withdrawn) from the vial to the syringe.

Set No for no viscous or low viscous sample.

Set Yes for high viscous sample.

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1-7

AI/AS 3000 Set Up View

AI/AS 3000 Method Editor________________________________________________________________________

Sampling depth in vial:

Determines the penetration depth of the syringe needle into the vial.

When Bottom is set (default value) the syringe needle penetrates up to the bottom of the vial.

When Center is set, the syringe needle penetrates up to the half of the vial.

Injection

Injection Group Box

This group box contains the parameters to control the penetration depth of the needle into the injector and the time the syringe needle stays in the injector before or after the sample is injected.

Injection depth:

It determines the penetration depth of the syringe needle into the vial.

When Standard is set (default value) the syringe needle penetrates into the injector up the maximum depth limit.

When Minimum is set, the syringe needle penetrates into the injector up to go beyond the septum only. .

Pre-inj. dwell time (s)

Enter the time in seconds the syringe needle is held in the inlet before the injection of sample. When you specify a delay of more than a few seconds, the needle preheats, which makes a Hot Needle Injection.

Set the wait time from 0 to 63 seconds.

Post-inj. dwell time (s)

Enter the time in seconds the syringe needle is held in the after the injection of sample.

A delay of more than a few seconds will heat the needle with the possible effect of driving those heavier analytes that have condensed in the needle out. A solvent flush technique may work better.

Set the wait time from 0 to 63 seconds.

Washes

Washes Group Box

These sections control the sample and pre- and post-rinses used to clean the syringe.

Controls displayed in this group box are for Pre Injection, Sample, and

Post Injection.

Pre Injection Group Box

The parameters used to pre-rinse the syringe before drawing up the sample.

1-8

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AI/AS 3000 Set Up View

____________________________________________________________________ __ AI/AS 3000 Method Editor

Solvent

Select which of the four solvent vials or the combination of two solvent vials will be used as the pre-rinse solvent choosing between the following options.

A, B, C, D, A+B, C+D.

Different solvent vials combinations than A+B or C+D are not possible.

Cycles

The number of times the solvent rinses the syringe. Select a number from the pull-down list box.

Sample Box

Use this control to pre-rinse the syringe with sample before actually pulling up the sample for injection.

Rinses:

The number of times the sample rinses the syringe. Select a number from 0 to

15.

Post Injection Box

The parameters used to rinse the syringe after the sample has been injected.

Solvent

Select which of the four solvent vials or the combination of two solvent vials will be used as the post-rinse solvent choosing between the following options. A, B, C, D, A+B, C+D.

Different solvent vials combinations than A+B or C+D are not possible.

Cycles

The number of times the solvent rinses the syringe. Select a number from the pull-down list box.

AI/AS3000 Command Menu

This menu allows to access the following function

Get Method from

Sampler

Send Method to

Sampler

This function allows transferring method data from autosampler to

GC.

This function transfers the analytical method from the memory of the

GC to the autosampler and enables the method.

Start Clean Cycle

This function permits to start a clean cycle.

Inject Single Sample

This function permits to start an single sample injection.

Force Autoinjector to

Stand-by

This function interrupts any current operation on the sampler putting it in stand-by condition.

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AI/AS 3000 Set Up View

Setting AI/AS 3000 Parameters ___________________________________________________________________

1.3 Setting AI/AS 3000 Parameters

1. In the Sampling group box, specify values in Sample fields.

a. In the Sample volume box, specify the volume of sample injected.

b. In the Plunger strokes box, specify the number of bubble elimination strokes for the syringe plunger. c. In Viscous box, specify if the sample is viscous.

d. In Sampling depth in vial box, specify the penetration depth of the syringe needle into the vial.

2. In the Injection group box, specify values in the Injection fields.

3. In the Sample box, specify the number of times the sample rinses the syringe.

4. In the Pre-Injection group box, select Solvent A, B, C, D, A+B or C+D.

a. Specify the amount of solvent used to rinse the syringe in the

Solvent field. b. Specify the number of times to rinse the syringe in the cycles field.

5. In the Post-Injection group box, select Solvent A, B, C, D, A+B or C+D. a. Specify the amount of solvent used to rinse the syringe in the

Solvent field.

b. Specify the number of times to rinse the syringe in the cycles field.

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