RUO Data Sheet for International

RUO Data Sheet for International
Cytomegalovirus (CMV)
Concentrated and Prediluted Cocktail Antibody
Control Number: 902-118-040215
Catalog Number:
CM 118 A, B, C
PM 118 AA
OAR 118 T60
Description:
0.1, 0.5, 1.0 ml, concentrated
6.0 ml, prediluted
60 tests, prediluted
Dilution:
1:50-1:100
Ready-to-use
Ready-to-use
Diluent:
Van Gogh Yellow
N/A
N/A
Intended Use:
For Research Use Only. Not for use in diagnostic procedures.
Summary and Explanation:
This antibody is a mixture of two monoclonal antibodies that reacts with immediate
early and early protein antigens in tissues infected with cytomegalovirus. This
antibody does not react with herpes virus or human papilloma virus. In the later stage
of infection, a cytoplasmic reaction may be observed.
Principle of Procedure:
Antigen detection in tissues and cells is a multi-step immunohistochemical process.
The initial step binds the primary antibody to its specific epitope. A secondary antibody
may be applied to bind the primary antibody, followed by an enzyme labeled polymer;
or an enzyme labeled polymer may be applied directly to bind the primary antibody.
The detection of the bound primary antibody is evidenced by an enzyme-mediated
colorimetric reaction.
Source: Mouse monoclonal
Species Reactivity: Human, others not tested
Clone: DT10 + BC90
Isotype: IgG2a + IgG1
Total Protein Concentration: ~10 mg/ml. Call for lot specific Ig concentration.
Epitope/Antigen: Cytomegalovirus proteins
Cellular Localization: Nuclear staining pattern with some cytoplasmic staining
Positive Control: CMV infected tissues
Known Applications:
Immunohistochemistry (formalin-fixed paraffin-embedded tissues)
Supplied As: Buffer with protein carrier and preservative
Storage and Stability:
Store at 2ºC to 8ºC. Do not use after expiration date printed on vial. If reagents are
stored under conditions other than those specified in the package insert, they must be
verified by the user. Diluted reagents should be used promptly; any remaining reagent
should be stored at 2ºC to 8ºC.
Staining Protocol Recommendations (manual use):
Peroxide Block: Block for 5 minutes with Biocare's Peroxidazed 1.
Pretreatment Solution (recommended): N/A
Pretreatment Protocol: N/A
Digestion Method:
Digest with Pepsin enzyme for 5 minutes at 37°C or for 15 minutes at room
temperature.
Protein Block (Optional): Incubate for 5-10 minutes at RT with Biocare's Background
Punisher.
Primary Antibody: Incubate for 30 minutes at RT.
Probe: Incubate for 10 minutes at RT with a secondary probe.
Polymer: Incubate for 10 minutes at RT with a tertiary polymer.
Chromogen: Incubate for 5 minutes at RT with Biocare's DAB - OR - Incubate for 5-7
minutes at RT with Biocare's Warp Red.
Counterstain: Counterstain with hematoxylin. Rinse with deionized water. Apply
Tacha's Bluing Solution for 1 minute. Rinse with deionized water.
Protocol Recommendations (ONCORE Automated Slide Staining System):
OAR118 is intended for use with the ONCORE Automated Slide Staining System.
Refer to the ONCORE Automated Slide Staining System User Manual for specific
instructions on its use. Protocol parameters in the ONCORE Automated Slide Stainer
Protocol Editor should be programmed as follows:
Protocol Name: CMV
Protocol Template (Description): Ms HRP Template 1
Dewaxing (DS Option): DS Enzyme
Antigen Retrieval (AR Option): AR2, low pH; 80°C
Reagent Name, Time, Temp.: CMV, 30 min., 25°C
Technical Note:
This antibody has been optimized for use with Biocare's MACH 4 Universal HRPPolymer Detection and ONCORE HRP Detection. Other Biocare polymer detection
kits may be used; however, users must validate incubation times and protocols for their
specific application. Use TBS for washing steps.
Limitations:
This product is provided for Research Use Only (RUO) and is not for use in diagnostic
procedures. Suitability for specific applications may vary and it is the responsibility of
the end user to determine the appropriate application for its use.
Precautions:
1. This antibody contains less than 0.1% sodium azide. Concentrations less than 0.1%
are not reportable hazardous materials according to U.S. 29 CFR 1910.1200, OSHA
Hazard communication and EC Directive 91/155/EC. Sodium azide (NaN3) used as a
preservative is toxic if ingested. Sodium azide may react with lead and copper
plumbing to form highly explosive metal azides. Upon disposal, flush with large
volumes of water to prevent azide build-up in plumbing. (Center for Disease Control,
1976, National Institute of Occupational Safety and Health, 1976) (2)
2. Specimens, before and after fixation, and all materials exposed to them should be
handled as if capable of transmitting infection and disposed of with proper precautions.
Never pipette reagents by mouth and avoid contacting the skin and mucous membranes
with reagents and specimens. If reagents or specimens come in contact with sensitive
areas, wash with copious amounts of water. (3)
3. Microbial contamination of reagents may result in an increase in nonspecific
staining.
4. Incubation times or temperatures other than those specified may give erroneous
results. The user must validate any such change.
5. Do not use reagent after the expiration date printed on the vial.
6. The SDS is available upon request and is located at http://biocare.net/.
Technical Support:
Contact Biocare's Technical Support at 1-800-542-2002 for questions regarding this
product.
References:
1. Vago L, et al. Coinfection of the central nervous system by cytomegalovirus and
herpes simplex virus type 1 or 2 in AIDS patients: autopsy study on 82 cases by
immunohistochemistry and polymerase chain reaction. Acta Neuropathol. 1996 Oct;92
(4):404-8.
2. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22,
Atlanta, GA. April 30, 1976 "Decontamination of Laboratory Sink Drains to Remove
Azide Salts."
3. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory
Workers from Occupationally Acquired Infections; Approved Guideline-Fourth Edition
CLSI document M29-A4 Wayne, PA 2014.
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