Operator`s Manual
Operator’s Manual
The Bullet Blender®
Models: Bullet Blender®; Bullet Blender® Storm; Bullet
Blender® Blue; Bullet Blender® 5 Storm and Bullet Blender® 5 E
Congratulations!
Congratulations on your purchase
®
of a Bullet Blender by Next Advance,
Inc., for lysing, disrupting, and
homogenizing your samples.
Please read this operator’s manual
which explains proper operation of the
instrument. This manual is posted on
our website, www.nextadvance.com.
Click the SUPPORT button on the lefthand menu bar and then on the
appropriate link to the manual.
We’re confident that your Bullet
Blender will become an essential tool in
your laboratory and we wish you
success with your work.
Contents
Parts of the Bullet Blender...................2
Setup.....................................................3
Operation..............................................3
Protocols and Sample Settings.............4
Cleaning...............................................5
Troubleshooting...................................5
Support.................................................5
Specifications.......................................5
Warranty..............................................6
Warnings and Cautions .......................6
Disclaimer............................................7
Contact Info..........................................8
Operator’s Manual for the Bullet Blender®
Parts of the Bullet Blender®
SYMBOLS USED ON THE BBX AND BBY MODEL BULLET BLENDERS
Start Operation
Caution: Follow the Instructions in the Operator’s Manual
This product complies with European Low Voltage and EMC Directives
Please dispose the test tubes and the BBX or BBY Bullet Blender in
accordance with local regulations
2
Operator’s Manual for the Bullet Blender®
SETUP
Place the Bullet Blender on a stable,
level lab bench. Plug the cord from the
AC/DC power adapter into the 24V
socket on the back of the Bullet Blender
and then insert the plug into a wall
outlet. You should see lights on the
time setting flash for several seconds,
indicating that the unit is receiving
power. It is now set up.
OPERATION
To begin using your BBX- or BBY
series Bullet Blender, lift open the
cover and insert the appropriate tubes.
For BBX24 models (BBX24 and
BBX24B), place up to twenty four
closed 1.5 mL to 2.0 mL polypropylene
®
high quality snap-cap (e.g. Eppendorf
Safe-Lock™) microcentrifuge tubes
containing samples into the sample
plate, and close the cover. When using
2.0 mL tubes we recommend using at
least two tubes per homogenization
cycle.
In the Bullet Blender 5 Storm
(BBY5M), load up to twelve 5 mL
®
Axygen brand screw-cap tubes. In the
Blender 5 E (BBY5E), load up to
®
twelve 5 mL Eppendorf tubes.
For the Bullet Blender Storm
(BBY24M), use up to 24 Next Advance
®
RINO screw-cap tubes or 1.5 mL
®
Eppendorf Safe-Lock snap-cap tubes.
In order to use these tubes, the Bullet
Blender must be fitted with the
appropriate gasket corresponding to the
tube type. Each gasket is clearly labeled
“RINO” or “Eppendorf”. The gasket is
removed by unscrewing the three
screws with a Phillips head screw driver
and lifting the gasket off of the lid.
Attach the proper gasket by aligning the
holes in the gasket with the holes in the
lid and inserting the screws. Make sure
that the gasket is screwed on securely,
or else homogenization efficiency may
be affected. Do not over-tighten the
®
screws. Only RINO screw-cap tubes
are recommended for use in the Bullet
Blender outfitted with the RINO™
gasket; other screw-cap tubes may
break or result in sub-optimal
homogenization.
The
snap-caps
on
the
microcentrifuge tubes must be closed
securely before using in the Bullet
Blender. The threads on all screw-cap
tubes must be dry and the caps must be
screwed on very tightly before using in
the Bullet Blender.
Do not operate with the cover
open. There will be excess noise, your
samples will not be properly processed
and the tubes may be knocked entirely
out of the instrument potentially
causing injury. If you turn the
“minutes” knob to “0”, the instrument
will stop.
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Operator’s Manual for the Bullet Blender®
EXAMPLE:
The figure above shows the Bullet
Blender set to run for 4 minutes at a
speed of 8, after running for 2 minutes.
To operate, press the “START” button.
After the first minute, the LED behind
the number 1 on the “minutes” dial will
light up. After the second minute, the
second LED behind the number 2 will
light up, and so on. At the settings
shown above, after 4 minutes, the
Bullet Blender will stop. In the Blue
and Storm modles, the words “Air
Cooling™” will light up in blue during
operation and for about one half minute
afterward as the fan continues to
operate.
Note that the Bullet Blender Storm
models have additional time settings for
15 and 30 second pulses. In addition,
the Bullet Blender Storm has an
additional, more powerful speed setting
of 12.
PROTOCOLS AND SAMPLE
SETTINGS
The following ratio should be used
as a guideline for determining the
amount of beads and buffer to use given
a certain sample size - 1 volume/mass
of tissue: 1 volume of beads: 2 volumes
of buffer. For more specific information
regarding the use of various beads as
well as specific protocol information,
please
refer
to
our
website
www.nextadvance.com.
As the tissue amount becomes
smaller, the above recommended ratio
may differ due to the limitations of
handling
small
volumes.
With
microcentrifuge tubes, we recommend
using a minimum of 25 μL of buffer
regardless of your sample size. For the
5 mL tubes, we recommend a minimum
volume of 100 μL.
With microcentrifuge tubes, the
recommended maximum sample mass
is 300 mg of organ tissue or 300 μL of
plant tissue or pelleted cell culture per
tube in the Bullet Blender. The tube
should not be filled more than twothirds of the way after the addition of
all contents (sample, beads, and buffer).
This is because the mechanics of
homogenization require empty space in
the tube. For 5 mL tubes, the
recommended maximum sample mass
is 1 g of organ tissue or 1 mL of plant
tissue or pelleted cell culture per tube.
Do not operate with more than a total of
3 mL combined buffer, sample and
beads per tube.
Cutting the tissue into smaller
pieces will generally yield better
results. Tissue with a high aspect ratio
(long, thin strips) will homogenize
better than tissue that is round or cubic.
Do not operate the Bullet Blender
using the same tubes for longer than 15
minutes.
Protocols for many types of samples are
posted on our website:
http://protocols.nextadvance.com.
4
Operator’s Manual for the Bullet Blender®
NOTES:
The Bullet Blender Storm 5 is
®
designed to work with 5 mL Axygen
brand tubes only. At high speed
settings, there may be some flaking of
the tubes. This is a normal side effect of
homogenization. The higher speed
enables homogenization of tougher
tissue.
CLEANING
If you wish to clean your Bullet
Blender, clean the outside of the unit
only with mild soap, water and a soft
cloth. Under normal conditions, the
Bullet Blender should never need to be
disassembled for cleaning. In the case
of a large spill, unplug the instrument,
remove the sample tube plate with a
1/8” hex wrench, wipe out the spill
using standard laboratory safety
precautions, and replace the sample
tube plate. Do not touch or tamper with
the electronics.
Contact tech support for a detailed
protocol: [email protected]
TROUBLESHOOTING
In addition to the tips given below,
thorough list of troubleshooting tips is
at http://bbtroubleshooting.nextadvance
.com
If the Bullet Blender doesn’t start, the
plug of the power supply cord may not
be in a live wall outlet or the power
supply connector may not be fully
inserted in the socket on the back of the
Bullet Blender.
If the unit stops working, turn the
system off for 15 minutes to allow the
electronics to reset. If the Bullet
Blender does not turn on after this
period, contact customer service.
If the caps on snap-cap tubes pop
open or the caps on screw-cap tubes
loosen, make sure that the interface
regions or screw threads between the
lids and the caps is dry when you close
the caps or screw them on, so that there
is enough friction for the caps to remain
tight. Using recommended types of
tubes will minimize cap failure.
SUPPORT
FAQs, protocols, and other helpful
information are available on our
website, http://www.nextadvance.com.
Click on the Bullet Blender, then on the
appropriate link. If you cannot find an
answer there, please contact customer
service
by
email
at
support
@nextadvance.com or by telephone at
1.518.674.3510 or (800) 738-1681.
SPECIFICATIONS
Size: 28 cm (11 in.) deep x 23 cm (9
in.) wide x 21.5 cm (8.5 in.) high.
Weight: 4 to 5 kg (8.5 to 11 lbs.),
depending upon model.
Power Requirement: BBX24 and
BBX24B models: 24 VDC, 0.75 Amp
BBY24M BBY5E and BBY5M
models: 24 VDC, 2.5 Amp
Capacity: 24 of 1.5 - 2.0 mL polypropylene high quality (e.g.
®
Eppendorf ) snap-cap tubes, 24 of 1.5
®
mL RINO screwcap tubes, 12 of 5 mL
®
Eppendorf tubes or 12 of 5 mL
®
Axygen 5 mL transport tubes,
depending upon model.
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Operator’s Manual for the Bullet Blender®
Relative Humidity: 5 – 90% non
condensing
Operating Temperature: 4 - 40°C
Altitude: <2000m
Storage Temperature: -40 to 50°C
requirements (-CE models
Meets
only)
WARRANTY
Next Advance warrants its Products
against defects in materials and
workmanship for time periods which
vary according to the Product. Within
these time periods, Next Advance will
replace or repair, without charge to the
original purchaser, any part which is
defective.
Bullet Blender warranty = Two years
The warranty is void if the Product
is defective due to product accident,
product modification, exposure to
radiation other than for sterilization,
connection to an improper electrical
supply, lack of proper maintenance,
contamination, improper installation or
misuse. If the product is used in a
manner
not specified by the
manufacturer, the protection provided
by the equipment may be impaired. The
warranty shall also not apply to defects
arising from fire, flood, lightning or
other conditions unrelated to correct
operation of the Product.
Next Advance’s liability is limited,
at the company’s election, to (1) refund
of the original purchaser’s purchase
price for the Product (2) repair of the
Product, or (3) replacement of the
Product or defective parts. Evidence of
purchase by the original purchaser is
required. Next Advance may also
request documentation of proper
maintenance, if applicable.
Next Advance makes no other
warranty, expressed or implied, with
respect to its Products. NEXT
ADVANCE
MAKES
NO
WARRANTY RESPECTING THE
MERCHANTABILITY
OF
THE
PRODUCTS
OR
THEIR
SUITABILITY OR FITNESS FOR
ANY PARTICULAR PURPOSE OR
USE. Next Advance shall not be liable
for indirect, special, incidental or
consequential damages of any nature.
Any recovery for any claim shall be
limited to the original purchase price
for the product.
OPERATOR’S RESPONSIBILITY
Provide proof of purchase and provide
normal care and maintenance.
WARNINGS AND CAUTIONS
Read the user’s manual before
operating.
Do not open lid when Bullet Blender is
in use.
Do not insert fingers or objects other
than recommended tubes into sample
tube holes.
Use caution when closing Bullet
Blender lid- do not close on fingers.
When working with hazardous or
pathogenic samples, operate the Bullet
Blender in a biosafety cabinet or other
standard laboratory safety enclosure.
Use recommended tubes only.
6
Operator’s Manual for the Bullet Blender®
No user serviceable parts are inside of
the instrument.
For indoor use only.
Pollution Degree 2 per EN 61010-1.
Overvoltage Category II per EN 610101.
Enclosure Protection: Not Protected
Against the Ingress of Moisture.
Sound Pressure Level: up to 90dBA for
BBX24 models; up to 100dBA for
BBY24M, BBY5E and BBX5MB
models. Use hearing protective
devices that reduce exposure to below
85 dBA during prolonged exposure.
Do not immerse in liquid.
Before touching the Bullet Blender,
touch a bare metal surface to discharge
static electricity.
DISCLAIMER
Next Advance, Inc. makes no
representations
or
warranties,
expressed, statutory or implied,
regarding the fitness or merchantability
of this product for any particular
purpose.In no event shall Next Advance
be liable for incidental or consequential
damages. Next Advance, Inc. is not
liable for any damages, including but
not limited to, lost profits, lost savings,
or other incidental or consequential
damages arising from ownership or use
of this product, or for any delay in the
performance of its obligations under the
warranty due to causes beyond its
control.
Next Advance, Inc. also reserves the
right to make any improvements or
modifications to the product described
in this manual at any time, without
notice of these changes. Next
Advance, Inc. products are not
designed, intended, or authorized for
use in applications or as system
components intended to support or
sustain human life, as a clinical medical
device for humans, or for any
application in which the failure of the
product could create a situation where
personal injury or death may occur.
All brand and product names used in
this manual are the trademarks of their
respective owners.
NEXT ADVANCE INC. DOES NOT
GUARANTEE THE INTEGRITY
OF THE TUBES USED IN THE
BULLET BLENDER. TUBES THAT
ARE NOT RECOMMENDED BY
THIS MANUAL MAY CRACK OR
OPEN IF USED IN THE BULLET
7
Operator’s Manual for the Bullet Blender®
BLENDER. NEXT ADVANCE INC.
OPTIMIZES
THE
BULLET
BLENDER TO SPECIFIC TUBE
TYPES AND BRANDS AND
CANNOT
GUARANTEE
THE
SAFE USE OF ALL TUBES BEING
SOLD ON THE MARKET.
CONTACT INFO
Next Advance, Inc.
Averill Park, NY, USA
Telephone 518-674-3510
www.nextadvance.com
Email: [email protected]
[email protected]
[email protected]
8
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