Assisted Reproductive Technologies

Assisted Reproductive Technologies
Assisted
Reproductive
Technologies
w w w . c r y o b i o s y s t e m . c o m
Assisted Reproductive Technologies
2
Founded in 1987, Cryo Bio System is a division of
IMV Technologies, the world leader in animal reproduction
biotechnologies, based in L’Aigle, Normandy - France.
Cryo Bio System is exclusively dedicated to human life sciences and biodiversity.
Combined with IMV Technologies Group's expertise in cryopreservation of biological samples,
Cryo Bio System provides innovative solutions to the scientific community through a range of High
Security storage products for biorepositories, biobanks and biological resources centres in various
fields and applications:
G
G
G
G
G
G
G
G
G
Cancer research, epidemiological studies
Blood transfusion haemovigilance
Regenerative medicine: cell and genetic therapy, stem cells
Genomics, proteomics
Medical and pharmaceutical research
Medico-legal storage
Military medicine
Biodiversity: animal and vegetal germplasm conservation
Assisted Reproductive Technologies (ART).
Our international presence through subsidiaries (Italy, Netherlands, United States, China and India), and
a network of distributors in over 60 countries offers customers both high quality products and expert
technical support.
This catalog demonstrates our commitment to the Assisted Reproductive Technologies (ART) field with a complete product line that
meets, in particular, all requirements of cryopreservation.
The solutions we have designed for humans make it possible
to deep-freeze sperm, embryos, ovarian cortex, as well as to
vitrify oocytes and embryos at various stages. We also have
developed the unique concept of long-term high-security
cryopreservation, an essential guarantee of storage safety and
quality for all samples.
C O N T E N T S
ICI / IUI / IVF M
Recisperme
4
Isolating transport container
4
®
Makler counting chamber
5
Leja counting chamber
5
ICSI, holding and biopsy pipettes
6
Aspiglaire
7
Craft™ pump
7
Intracervical insemination catheter for CBS™ High Security straws
8
Intra-uterine insemination catheter with plunger
9
Vitrification M
HSV High Security Vitrification kit
10
Opening device for HSV kit
11
Cryopreservation M
CBS™ High Security cotton-plugged sperm straw
12
CBS™ High Security hydrophobic plugged embryo straw
14
CBS™ High Security unplugged tissue 2 mL straw
15
PETG cotton-plugged sperm straw
16
PETG cotton-plugged embryo straw
16
Accessories M
LabPal printer
17
Micro-aspirator
17
CBS™ visotubes and goblets
18
Visotubes and goblets
19
Canisters
20
Equipment M
21
SYMS II / SYMS
22
PACE
24
MAPI
25
Controlled rate freezers from Planer
26
Coming soon! M
27
M
SIDE
Assisted Reproductive Technologies
ICI / IUI / IVF
Recisperme
007426 Pack of 20 Recisperme
SPERM COLLECTION VESSEL
DESCRIPTION / USE
Spermogram, sperm culture, spermocytogram
(insemination, in vitro fertilization, diagnosis).
Sterilized by irradiation.
Individually packaged in box of 20 units.
TECHNICAL SPECIFICATIONS
The innovative design of the Cryo Bio System
Recisperme allows sperm to be collected directly
into a round bottom tube, eliminating the need
for sample transfer and enhancing sample quality
by avoiding tricky handling.
Furthermore, thanks to a graduated tube inside
the device the sperm volume can be accurately
read (contents 10 mL).
4
Preassembled sterile unit comprised of:
G a funnel-shaped upper part;
G a graduated 10 mL tube with a round base
and blue stopper;
G a support; and
G an adhesive cover.
INSTRUCTIONS FOR USE
G
After ejaculation directly into the vessel, the
graduated tube is held in place to facilitate
flow;
G
The adhesive cover can be put on to guarantee
that the sample remains sterile;
G
Then the funnel is removed to avoid potential
contamination, and the tube is closed with the
stopper and sent to the laboratory for analysis.
SHELF-LIFE
Three years from date of manufacture.
Isolating transport container
015937 Bag of 5 isolating transport containers
DESCRIPTION / USE
For transportation of prepared sperm prior to intrauterine insemination.
High density Polyethylene container with Polyurethane foam insert precut to hold one tube.
All commercially available 5 to 15 mL round or
conical bottom culture tubes fit the size of the
precut holder.
TECHNICAL SPECIFICATIONS
Length: 120 mm
Diameter: 65 mm.
Two hours prior to use, the container can be
placed at 37°C. This ensures that tubes filled with
prepared sperm are at an optimal temperature
for transportation from the laboratory to the clinic
where the insemination will be performed, guaranteeing sperm mobility by preventing thermal
shock.
Guarantees sperm mobility by avoiding thermal
shock.
INSTRUCTIONS FOR USE
G
Open the container by pulling up the lid;
G
Place the tube into the opening situated in the
middle of the foam insert;
G
Close the container by putting the lid back on.
The container is now ready to be transported to
the clinic for insemination.
If necessary, the container can be labeled for identification.
Maximum duration of transportation: 15 minutes.
®
Makler counting chamber
DESCRIPTION / USE
INSTRUCTIONS FOR USE
Rapid sperm analysis.
Easy-to-use: sperm count performed on undiluted specimen.
Counting chamber and cover glass for counting
and analysis of spermatozoa.
TECHNICAL SPECIFICATIONS
Reusable: easily cleaned with a non-bleach disinfectant solution. The depth of 10 microns eliminates
blurring and allows sperm to move freely. The
applied sample is observed in one focal plane.
A 1 mm² grid, subdivided into 100 squares, each
one of 0.1 x 0.1 mm, is on the cover glass, eliminating the need for insertion and removal of a
grid into the microscope eye.
016261 Makler ® counting chamber
(complete with cover galss and grid)
016262 Extra cover glass with grid
The number of spermatozoa counted in any strip
of 10 squares of the grid indicates their concentration in millions/mL.
No additional factors are necessary for calculation.
Observation of color fringes at the four contact
points, provides a self-controlled test for accuracy.
The cover glass can never be raised by the
applied sample.
Repeated use is possible with complete accuracy
and without further calibration.
5
LEJA counting chamber
019319 Box of 25 disposable Leja slides – 2 chambers
019320 Box of 25 disposable Leja slides – 4 chambers
DESCRIPTION / USE
High quality disposable counting chambers for
semen analysis. Due to their special characteristics, Leja slides will both save time and increase
the level of accuracy and precision of semen
analysis.
All slides are covered with a special coating to
prevent air bubble formation and to prevent
semen from sticking to the chamber surface. The
resin and ink used are both non-toxic.
The Leja slides have an excellent low limit of
quantification and can handle high sperm concentrations. This has a direct clinical impact: you will
be better prepared to determine the thresholds
for the various modalities of Assisted Reproduction
Technologies (waiting for natural conception,
intrauterine insemination, in-vitro fertilization or
intracytoplasmic sperm injection) and to decide
which treatment is optimal for a couple.
The high level of accuracy and precision of the Leja
chambers will allow your results to be compared
with other laboratories’ scientific data concerning
the relationship between semen parameters
(concentration, motility, viscosity) and fertility.
TECHNICAL SPECIFICATIONS
Slides with 2 or 4 chambers
Depth: 20 microns
Assisted Reproductive Technologies
ICI / IUI / IVF
ICSI, holding and biopsy pipettes
022166
022167
ICSI pipette – angle 20°
ICSI pipette – angle 30°
10
10
ICSI pipettes
DESCRIPTION / USE
ICSI injection pipettes are used to perform intracytoplasmic sperm injection (ICSI).
TECHNICAL SPECIFICATIONS
The unique shape and taper of our ICSI pipettes
provide for smooth movement of its content. A
few sperm can be loaded for injecting a few
oocytes at one time. The short and sharp fine
spike at the tip allows for easy, and non- traumatizing penetration of the oocyte.
The 50V series is our classic design with an
internal diameter of 5 µm, a spiked tip bevelled
at 30°. They are supplied with an angle of 20° or
30° with a tip to elbow length of 550 µm.
Individually packaged in box of 10 units.
6
SHELF-LIFE
Three years from date of manufacture.
022168
022169
022170
022171
Holding pipette – outer diameter 100 µm – angle 20°
Holding pipette – outer diameter 100 µm – angle 30°
Holding pipette – outer diameter 120 µm – angle 20°
Holding pipette – outer diameter 120 µm – angle 30°
10
10
10
10
Holding
pipettes
DESCRIPTION / USE
Holding pipettes are used to hold an oocyte or an
embryo in position during ICSI and other micromanipulation procedures.
TECHNICAL SPECIFICATIONS
The classic series has an internal diameter of
20 µm and an outer diameter of 100 µm or
120µm.
All our holding pipettes are supplied with a tip
angle of 20° or 30° with a tip to elbow length of
650µm.
Individually packaged in box of 10 units.
SHELF-LIFE
Three years from date of manufacture.
022172
Pipette for blastomere biopsy – angle 30°
10
Blastomere
biopsy pipettes
DESCRIPTION / USE
Blastomere biopsy pipettes are designed to
remove blastomeres from embryos for preimplantation genetic diagnosis (PGD).
TECHNICAL SPECIFICATIONS
They are supplied with a tip angle of 30° and a
tip to elbow length of 550µm.
Individually packaged in box of 10 units.
SHELF-LIFE
Three years from date of manufacture.
Aspiglaire
DESCRIPTION / USE
Hühner test (post-coital sample collection).
Mucus viscosity evaluations.
Endo- or exocervical mucus sampling.
Sterilized by irradiation.
Individually packaged in quantities of 1 or 5 units
in box of 20 or 40 units.
ENDOCERVICAL ASPIRATOR FOR HÜHNER TEST
007447
Box of 20 Aspiglaire (individually packaged)
007448
Box of 40 Aspiglaire (8 cells containing 5 units)
CE marked
7
TECHNICAL SPECIFICATIONS
The Aspiglaire catheter has two components:
G a clear, flexible sheath with a round tip; and
G an inner blue plunger.
No syringe needed.
Length: 280 mm
External diameter: 2.92 mm
INSTRUCTIONS FOR USE
G Slowly insert the catheter into the cervical
canal until reaching the endocervical sampling
site;
G Aspirate the cervical mucus by pulling the
plunger which automatically stays in the
desired position to allow the mucus to be
drawn into the tube;
G Gently remove the catheter from the cervix;
G Apply the sample on a slide for examination.
SHELF-LIFE
Three years from date of manufacture.
Rocket Craft™ Pump
005553
Rocket Craft™ pump - Vacuum pump for oocyte recovery
015621
Pedal for Craft™ pump
006553
Craft™ pump tubing
018204
Craft™ pump overflow reservoir
018268
Specific cap for Craft™ pump reservoir
019270
Luer-Lock tubing adaptor for Craft™ pump tubing
DESCRIPTION / USE
TECHNICAL SPECIFICATIONS
Oocyte aspiration.
Vacuum is activated by a foot operated switch
controlled by the surgeon performing oocyte
collection.
The Craft™ pump is specifically designed to
provide a smooth low volume vacuum at a predetermined negative pressure.
12-month warranty.
Dimensions: 20 x 14 x 12 cm
Weight: 3.2 kg
Vacuum: 0 - 400 mm Hg
CE marked
Assisted Reproductive Technologies
ICI / IUI / IVF
8
Intracervical insemination catheter
FOR CBS™ HIGH SECURITY SPERM STRAWS
CE marked
015821
Box of 20 sterile ICI catheters for CBS™ High Security sperm straws
DESCRIPTION / USE
INSTRUCTIONS FOR USE
Artificial intracervical insemination with thawed
sperm.
G
Thaw the straw and release the safety plug by
pinching it between finger and thumb;
For use with CB™ 0.3 and 0.5 mL straws.
G
Decontaminate the straw with a bactericidal
agent;
G
Cut of the seal at the plug side of the straw;
G
Place the straw in the clear sheath, sample
side first;
G
Make sure the opening of the straw is correctly
positioned in the sheath;
G
Introduce the catheter into the cervical canal
and insert the plunger inside the guide;
G
Gently push away the plug of the straw and
inject the sperm very slowly.
Sterilized by irradiation.
Individually packaged in box of 20 units.
TECHNICAL SPECIFICATIONS
The intracervical insemination catheter consists
of 3 parts:
G a flexible PVC outer sheath with safety sleeve.
Length: 24.8 cm
Internal diameter: 3.7 mm
External diameter: 4.1 mm
G
G
a white PETG guide to position the straw in
the outer sheath.
Length: 13 cm
Internal diameter: 2.5 mm
External diameter: 3.1 mm
and a white PETG plunger to expel the contents
of the straw.
Length: 28 cm
External diameter: 1.5 mm
SHELF-LIFE
Three years from date of manufacture.
Intra-uterine insemination catheter
with plunger
CE marked
007446
Box of 20 sterile IUI catheters with plunger
DESCRIPTION / USE
Intra-uterine insemination with processed sperm.
Sterilized by irradiation.
Individually packaged in box of 20 units.
TECHNICAL SPECIFICATIONS
The intra-uterine insemination catheter consists
of 2 parts:
G a clear flexible PVC sheath equipped with
G a thin and flexible cannula with a soft tip for
easy entry into the uterine cavity.
No syringe needed.
A plastic plug guarantees sterility when the
processed sperm must be transported to the clinic
prior to insemination.
Length: 28 cm
External diameter: 3 mm
External diameter of the tip: 1 mm
INSTRUCTIONS FOR USE
G
Pull on the plunger to draw the required
volume of sperm into the catheter;
G
Insert a speculum;
G
Introduce the catheter into the cervical canal,
then through the internal cervical orifice;
G
Slowly inject the sperm into the uterine cavity;
G
Keep the patient lying down for a few minutes.
SHELF-LIFE
Three years from date of manufacture.
9
Assisted Reproductive Technologies
Vitrification
HSV High Security Vitrification kit
DESCRIPTION / USE
Vitrification of oocytes and embryos.
The HSV High Security Vitrification kit is comprised
of 3 parts:
G a High Security ionomeric resin straw;
G a capillary tube with a pre-formed gutter attached to a colored handling rod; and
G a blue plastic insertion device.
10
Sterilized by irradiation.
Packaged in a peel off blister pack (4 kits).
IDENTIFICATION
The HSV kit can be labeled using LN2 resistant
labels.
FILLING AND SEALING
Pack of 4 sterile HSV High Security Vitrification kits
019916
red
019917
white
019918
yellow
019919
purple
019920
blue
019921
green
022406
Opening device for HSV kit
G
Prepare the identification label (liquid nitrogen
resistant) for the HSV straw and apply it
approximately 20 mm (0.8 inch) from the flared
end of the straw;
G
Connect the end of the blue plastic insertion
device to the colored end of the handling rod;
G
Prepare the sample for vitrification according
to laboratory protocol;
G
Using a micro-pipette, carefully deposit the
sample into the gutter one millimeter from the
end. The drop holding the sample must be
under 0.5 µL. Maximum of 2 oocytes or
embryos. In this case, use a droplet by sample;
G
Immediately place the pre-formed gutter with
handling rod in the flared end of the straw.
Push until the rectangular portion of the blue
handler comes into contact with the flared end
of the straw;
G
Slightly pinch the straw between your thumb
and finger and remove the insertion device;
G
While still holding the straw in place, seal the
open end using a SYMS sealer;
G
Hold the straw using tweezers in the area of
the handling rod;
G
Quickly plunge the entire straw into liquid
nitrogen vertically;
G
Gently stir the straw in liquid nitrogen for a few
seconds so as to avoid formation of an isolating
air bubble layer around the straw.
CE marked
FDA 510(k) premarket notification
clearance # K092398
for 4-8 cell and blastocyst stage embryos
STORAGE IN LIQUID NITROGEN
Cryo Bio System carries a line of accessories for
storage and management of HSV kits in liquid
nitrogen.
11
THAWING
Thawing of the specimen should be done in a
controlled environment, such as a laminar flow
hood.
G
Prepare the thawing / dilution media;
G
Identify the HSV straw;
G
Transfer the straw from the storage container
to a transport dewar filled with liquid nitrogen;
G
Lift the straw enough to expose the colored
handling rod. Make sure the end with the
sample remains immersed in the liquid
nitrogen;
G
While holding the straw, use the opening device
for HSV kit to section the straw;
G
Immediately (within 2 seconds), plunge the
gutter into the first dilution media.
SHELF-LIFE
Three years from date of manufacture.
Opening device for HSV kit
Assisted Reproductive Technologies
Cryopreservation
12
CBS™ High Security cotton-plugged sperm straws
DESCRIPTION / USE
IDENTIFICATION
Storage and preservation of sperm.
The CBS™ 0.3 mL and 0.5 mL straws carry
tamper-proof barcode and/or alphanumeric identification on an outer jacket that can be removed
after thawing, guaranteeing sample traceability.
The special design and the mode of filling and
sealing of CBS™ High Security straws for cryopreservation of human biological samples makes
them fully hermetic, especially in liquid nitrogen.
Manufactured from biocompatible materials, they
are used in medically assisted procreation techniques and particularly for sperm preservation.
The CBS™ straw is a clear, flexible tube made
of ionomeric resin.
Length: 133 mm
Internal diameter: 2.25 mm
Sterilized by irradiation.
Model CBS™ 0.3 mL
This straw is composed of two distinct parts separated by a sliding white cotton safety plug. The
first part with a working volume of 0.3 mL holds
the sperm sample, while the second permits introduction of a colored identification tube and serves
as a handle in liquid nitrogen.
Model CBS™ 0.5 mL
Same technical specifications as the 0.3 mL
straw, except that the working volume is 0.5 mL,
and the outer part of the cotton safety plug is
colored.
FILLING AND SEALING
Straws are filled individually by means of a yellow
sterile nozzle and a micro-aspirator.
The impermeability of the seal is only guaranteed
if the CBS™ straws are sealed with the SYMS or
SYMS II sealing systems.
For large series (sperm banks), the CBS™ straws
are filled and sealed on an automatic machine
(PACE) or on an automatic filling, sealing and printing machine (MAPI).
STORAGE IN LIQUID NITROGEN
Cryo Bio System carries a line of accessories for
storage and management of CBS™ straws in
liquid nitrogen.
RECOMMENDATIONS
FOR THAWING CBS™ STRAWS
After verifying the location of the desired straw,
its identification is confirmed by the color of the
internal identification tube (CBS™ 0.3 mL), its
plug (CBS™ 0.5 mL) and/or its outer jacket.
The straw is gently removed from the storage
visotube, taking care to keep the part containing
the sperm in liquid nitrogen. Once it is checked,
the straw is reheated by placing it in warm water
(maximum 37°C) for a few seconds. After drying,
it is ready to use.
SHELF-LIFE
Three years from date of manufacture.
CE marked
FDA 510(k) premarket notification clearance # K002595
010287 CBS™ High Security sperm straw 0.3 mL with white cotton plug*
010288
20
100
CBS™ High Security sperm straw 0.3 mL with white jacket and colored fixed rod
017797 red
017798
white
017799
yellow
017800 blue
017801
green
017802
purple
014651 CBS™ High Security sperm straw 0.5 mL with white cotton plug*
75
20
014650
100
CBS™ High Security sperm straw 0.5 mL with colored cotton plug*
100
red
016611
yellow
016612
green
016613 blue
016584
016614
orange
016615
grey
13
008656 Bag of 10 sterile yellow filling nozzles
Bag of 10 weighted identification rods 30 mm
019021 yellow
019022
green
019023
blue
019024 orange
019025
white
019026
red
Identification jacket
50
010272 red
010273
white
010274
orange
010275 green
010276
blue
010277
yellow
014939 clear
014175 Set of 2 indelible black markers
4
016730 Sterile connection tubing for syringe (individually packaged)
1
016731 Sterile connection tubing for syringe (packaged by 5)
010284 Sterile emptying device
5
20
Assisted Reproductive Technologies
Cryopreservation
14
CBS™ High Security hydrophobic plugged
embryo straw
CE marked
FDA 510(k) premarket notification clearance # K002595
010286
20
CBS™ High Security embryo straw with hydrophobic plug and clear filling nozzle preconnected
DESCRIPTION / USE
IDENTIFICATION
Storage and preservation of embryos.
The CBS™ High Security embryo straw carries
a tamper-proof colored identification rod to
guarantee sample traceability.
The special design and the mode of filling and
sealing of CBS™ High Security straws for cryopreservation of human biological samples makes
them fully hermetic, in ultra low temperatures.
Manufactured from biocompatible materials, they
are used in medically assisted procreation techniques and particularly for embryo preservation.
The CBS™ embryo straw is a clear, flexible tube
made of ionomeric resin.
Length: 133 mm
Internal diameter: 2.25 mm
FILLING AND SEALING
The CBS™ High Security embryo straw is filled
by aspiration using a micro-aspirator according
to a specific protocol.
The impermeability of the seal is only guaranteed
if the CBS™ straws are sealed with the SYMS or
SYMS II sealing systems.
STORAGE IN LIQUID NITROGEN
Its disposable, clear filling tip allows introduction
of the embryo without contaminating the opening.
Two distinct parts separated by a hydrophobic
plug guarantee embryo sterility during introduction
and recovery after thawing.
The first part holds the embryo and the adjacent
dilution solvent (working volume 0.3 mL), while
the second part allows insertion of a colored identification rod which also facilitates easy identification
in liquid nitrogen.
Sterilized by irradiation.
Cryo Bio System carries a line of accessories for
storage and management of CBS™ straws in
liquid nitrogen.
RECOMMENDATIONS
FOR THAWING CBS™ STRAWS
After verifying the location of the desired straw,
its identification is confirmed by the color of the
identification rod.
SHELF-LIFE
Three years from date of manufacture.
Individually packaged in blister of 20 units.
Weighted identification rod 40 mm
10
010266 yellow
010267
green
010268
blue
010269 orange
010270
white
010271
red
CBS™ High Security unplugged tissue 2 mL straws
CE marked
018960
20
CBS™ High Security tissue 2 mL straw
15
DESCRIPTION / USE
FILLING AND SEALING
STORAGE IN LIQUID NITROGEN
Cryopreservation of tissue fragments.
Filling and sealing of the straw should be done
in a controlled environment, such as a laminar
flow hood.
Cryo Bio System carries a line of accessories for
storage and management of CBS™ straws in
liquid nitrogen.
Sterilized by irradiation.
Individually packaged in blister of 20 units.
G
IDENTIFICATION
The CBS™ High Security tissue straws can be
identified by using LN2 resistant labels.
Prepare the identification label and apply it on
the outside of the CBS™ High Security tissue
2 mL straw;
G
Holding the straw near the jaws, seal one
extremity of the straw with the sealer SYMS
equipped with the special guide for CBS™
High Security tissue 2 mL straws or SYMS II;
G
Using a tong, carefully deposit the tissue fragment(s) into the straw, according to laboratory
protocol (fragments or wrapped pieces);
G
Holding the straw near the jaws, seal the
other extremity of the straw with the SYMS or
SYMS II sealing systems.
SHELF-LIFE
Three years from date of manufacture.
Assisted Reproductive Technologies
Cryopreservation
16
PETG cotton-plugged sperm straws
CE marked
010261 Sterile PETG 0.25 mL sperm straw
014550 Sterile PETG 0.5 mL sperm straw
DESCRIPTION / USE
IDENTIFICATION
Freezing and storage of human sperm.
PETG sperm straws are clear flexible straws made
of a copolymer.
The straws can be printed by ink jet printer or
identified by special LN2 labels or indelible marker
for storage in liquid nitrogen.
G
0.25 mL straw
Length: 133 mm
Internal diameter: 1.6 mm.
FILLING AND SEALING
0.5 mL straw
Length: 133 mm
Internal diameter: 2.5 mm.
For storage of sperm in preparation of intrauterine insemination or in vitro fertilization, the
PETG straws are filled with a micro-aspirator (or
with a syringe and an adapter) and closed with
short ID plugs of 13 mm in length.
G
They are composed of two distinct compartments
separated by a sliding safety plug.
G The larger compartment is designed to receive
sperm and has an effective volume of 0.21 mL
or 0.47 mL.
G The smaller compartment is designed as for
aspiration at the time of filling.
Sterilized by irradiation.
Packaged in bags of 100 units.
STORAGE IN LIQUID NITROGEN
Cryo Bio System carries a line of accessories for
storage and management of PETG straws in liquid
nitrogen.
Shelf-life
Three years from date of manufacture.
007342 Sterile obturation and identification rods (13 mm) yellow
20
PETG cotton-plugged embryo straws
CE marked
DESCRIPTION / USE
IDENTIFICATION
Freezing and storage of human embryos.
PETG embryo straws are flexible or rigid clear
tubes made of copolymer.
The PETG embryo straws are closed at one end
by two woven plugs enclosing a dose of powder
that polymerizes when in contact with liquid.
Their useful volume is:
G 0.15 mL for the 91 mm long straws and
G 0.25 mL for the 133 mm long straws.
The straws are identified by different colored
plugs.
The internal diameter is 1.6 mm for both models.
Sterilized by irradiation.
Packaged in blisters of 4 x 5 units.
FILLING AND SEALING
For embryo cryopreservation, the straws are filled
with the use of a micro-aspirator.
After the loading procedure, the open extremity
of the straw is closed by an obturation and an
identification colored plug which is 42 mm in
length for 91 mm straws, and 13 mm for 133 mm
straws.
Shelf-life
Three years from date of manufacture.
006433 Blister of 20 rigid embryo straws 91 mm
006578 Blister of 20 rigid embryo straws 133 mm
014102 Blister of 20 flexible embryo straws 91 mm
014103 Blister of 20 flexible embryo straws 133 mm
Blister of 20 obturation and identification rods (42 mm)
007437 yellow
007438
purple
007440
red
007442 orange
014484
pink
014485
blue
014486 grey
014487
green
014488
royal blue
014489 white
007342 Sterile obturation and identification rods (13 mm) yellow
20
Accessories
LabPal
DESCRIPTION / USE
TECHNICAL SPECIFICATIONS
Identification of CBS™ High Security straws and
HSV High Security Vitrification kits.
Print method: Thermal transfer (durable, nonsmearing)
Brady's laboratory labels are designed to withstand extreme laboratory environments.
Multiple line printing
12-month warranty.
Warning
Using our LN2 resistant labels, it is strongly
recommended to overlap the label on the straw.
019808 LabPal printer
Weight: 0.55 kg with batteries and tape cartridge
included
L x W x H: 228.6 x 114.3 x 63.5 mm
Power source: 6 AA-size batteries or AC with
optional adapter
Operating temperature: 4° - 49°C
FCC Class A approved (printer)
019810
019811
019812
019813
019859
AC adaptor 220V for LabPal printer
Hard case for LabPal printer
Polyester ribbon for LabPal printer LN2 resistant
Nylon tissue ribbon for LabPal printer LN2 resistant
Clear polyester ribbon for LabPal printer for identification rods
for CBS™ High Security straws
Micro-aspirator
014498 Micro-aspirator (delivered with one extra unit of tubing)
014500 Extra nozzle tubing for micro-aspirator
DESCRIPTION / USE
G
The micro-aspirator is used to manually fill all
straws manu-factured by Cryo Bio System without
any risk of contamination.
G
INSTRUCTIONS FOR USE
G
G
Hold the aspirator in a horizontal position.
Carefully push the straw into the adaptor and
aspirate the liquid. The tube length should be
about 10 cm.
Slowly turn the thumb wheel towards the
adaptor until reaching the correct filling height.
Remove the straw just underneath the adaptor.
No maintenance required.
17
Assisted Reproductive Technologies
Accessories
CBS™ visotubes and goblets
DESCRIPTION / USE
Freezing and storage of biological materials in
straws.
CBS™ High Security straws are placed in goblets
for storage in freezers. Goblets come in 8 different colors and their sub-compartments (called
visotubes) in 12 different colors.
In the Daisy configuration, goblets hold 12 visotubes of different colors and can hold a maximum
of 168 straws of 0.3 ml or 0.5 mL.
18
Diameter: 65 mm (67 mm with cover)
Goblet height: 117 mm
Total height of Daisy goblet (with cover): 135 mm
Visotubes height: 120 mm
135 mm
40 mm
70 mm
120 mm
133 mm
Height of visotube diameter 17.5 mm: 133 mm
Round
visotube
ø 17.5 mm
C
O
L
O
R
S
Triangular
visotube
Goblet with
pre-attached
cover
(height 4O mm)
(height 70 mm)
(bag of 1)
(bag of 5)
Cover for gobelet ø65 mm
Daisy goblet*
Clear
006924
-
021224
021366
006321
015144
015152
Black
-
018153
-
-
-
-
-
Brown
-
018147
-
-
-
-
-
Red
-
018146
021228
-
-
015146
015154
Green
-
018145
021226
-
-
015147
015155
Blue
-
018148
021225
-
-
015151
015159
Grey
-
018144
-
-
-
018141
018142
Purple
-
018152
021229
-
-
015150
015158
Yellow
-
018143
021227
-
-
015148
015156
Pink
-
018149
-
-
-
-
-
Orange
-
018150
-
-
-
-
-
Pistachio
-
018151
-
-
-
015149
015157
100
100
10
50
10
1
5
55
45
820
-
-
540
540
20
20
365
-
-
240
240
14
14
225
-
-
168
168
PACKAGING
C
0.25 mL classical straws
A
P
A
0.5 mL classical straws
C
T
0.3 mL & 0.5 mL CBS™ straws
Y
Visotubes and goblets
DESCRIPTION / USE
Freezing and storage of biological materials in
straws.
Arrangement using color coding of straws prior
to storage in liquid nitrogen.
G
Standard goblet Ø 35 mm
Diameter: 35 mm
Height: 122 mm
G
Standard goblet Ø 65 mm
Diameter: 65 mm
Height: 117 mm
G
19
Polygonal, hexagonal and round visotubes
Height: 120 mm
All visotubes are also available in bulk units of
300 (singular color/diameter); please contact us
for further information.
C
O
L
O
R
S
Polygonal
visotube
Hexagonal
visotube
Round
visotube
ø 7.1 mm
Round
visotube
ø 9.2 mm
Round
visotube
ø 10 mm
Round
visotube
ø 12 mm
Round
visotube
ø 13 mm
Goblet
ø 35 mm
Goblet
ø 65 mm
White
020470
020486
020492
020499
020505
020511
020517
020523
006418
Blue
020472
020487
020493
020500
020506
020512
020518
-
006755
Green
020482
020488
020494
020501
020507
020513
020519
-
006754
006756
Yellow
020483
020489
020495
020502
020508
020514
020520
-
Orange
020484
020490
020496
020503
020509
020515
020521
-
-
Red
020485
020491
020497
020504
020510
020516
020522
-
006397
Purple
-
022141
-
-
-
-
-
-
006757
Pistachio
-
-
-
-
-
-
-
-
006753
10
10
10
10
10
10
10
10
10
0.25 mL
classical straws
30
90
7
10
14
22
27
200
820
0.5 mL
classical straws
15
36
2
5
5
8
10
94
365
0.3 mL & 0.5 mL
CBS™ straws
8
22
1
3
4
7
7
75
225
in a goblet
ø 35 mm
4
1
15
10
8
5
5
-
-
in a goblet
ø 65 mm
15
7
60
37
31
21
19
-
-
PACKAGING
C
A
P
A
C
I
T
Y
Assisted Reproductive Technologies
Accessories
Canisters
Canister with handle and white cane for classical goblets ø 65 mm without cover
007147 2-shelf
007148
3-shelf
007150 5-shelf
007151
6-shelf
007149
4-shelf
Canister with handle and white cane for Daisy goblets
008644 2-shelf
008645
3-shelf
007198 5-shelf
007199
6-shelf
1
008646
4-shelf
Plastic cane (color flag)
20
10
20
007328 white
015693
yellow
015694
salmon
015695 pink
015696
dark green
015697
turquoise
015698 orange
015699
purple
015706
grey
014175 Set of 2 indelible black markers
4
005524 Short tongs (250 mm), stainless steel
1
005523 Long tongs (700 mm), stainless steel
1
DESCRIPTION / USE
Cryo Bio System canisters are the sleeves that
hold the goblets. They are compatible with all
types of liquid nitrogen and vapor freezers, and
also low-temperature mechanical freezers.
Five lengths of canisters are available, with a
capacity of 2 to 6 goblets stored on top of each
other. A colored flag can be placed on the lifter
to facilitate retrieval.
Canisters are placed in the nitrogen freezer before
filling the container with liquid nitrogen. Goblets
are introduced and retrieved one by one with the
metal lifter; all manipulation of goblets is in the
cold environment of the neck of the nitrogen
freezer to ensure the stable low temperature of
the samples.
Diameter: 70 mm.
Equipment
SIDE
AUTOMATIC PRINTING MACHINE FOR CBSTM HIGH SECURITY STRAWS
CE marked
DESCRIPTION / USE
SIDE machine cycle steps:
An automatic system for ink-jet printing of biological samples in CBS™ High Security straws.
G
Hopper feeding of straws;
G
Ink-jet printing of tamper proof bar code and
corresponding text of up to 10 digits and
control key (code 128);
G
Manual transfer to their storage address.
SIDE is compatible with CBS™ 0.3 or 0.5 mL
open or sealed straws and can automatically print
bar codes (code 128) and/or alphanumerical
identification on the jacket of the straws.
SIDE is a flexible identification system
enabling the laboratory to:
The ink-jet printing system requires active evacuation of its solvent vapors.
print the requested number of straws for a
sample;
Setup, maintenance and shutdown: 15 minutes
per day.
G
G
print the same code on the straws as the
sample to ensure the link and
G
print straws after sealing to ensure sterility of
the content.
SIDE pilot software
The SIDE software features bar code reading or
keyboard entry of codes to be printed and export
of production files in different formats.
TECHNICAL SPECIFICATIONS
G
SIDE
L x W x H: 500 x 450 x 190 mm
Weight: 20 kg
SIDE is provided with a Linx or Domino printer.
Power supply: 110 or 220 V / 60 or 50 Hz
SIDE guarantees a one-to-one identification relationship between the straw and the sample code.
SIDE automatic printing machine
for CBSTM High Security straws
The SIDE machine consists of 3 parts:
019651 220 V / with Linx printer
G
a straw handler;
G
a PC with pilot software;
G
and an ink-jet printing system.
019652 110 V / with Linx printer
018802 220 V / with Domino printer
018803 110 V / with Domino printer
21
Assisted Reproductive Technologies
Equipment
SYMS II
Unique sealer for CBS™ High Security straws and tubes
The SYMS II sealer is a bench top unit that can be used under
laminar flow hood and is using the « thermal » procedure to
weld CBS™ High Security 0.3 mL, 0.5 mL and 2 mL straws,
CBS™ High Security tubes as well as the HSV High Security
vitrification straw.
KEY FEATURES
G
Available in 3 languages
(French, English or Spanish)
G
Glove compatible touch screen interface
G
Easy-to-use touch screen advises user of the
machines actions: heating up, ready, welding
process, etc.
G
Pre-set specific combination of seal temperature, time and jaw position for each CBS™
device
G
Two sealing modes: fully automatic straw
detection system or touch screen operator
control
G
Error management: alarm message on touch
screen and audible signal
22
CE marked
022319 SYMS II sealing system for CBS™ High Security straws and tubes
022847 Spare Teflon strip
G
Electronic touch screen monitors and advises
on the maintenance needs of the machine
G
Easy to clean and maintain
INSTRUCTIONS FOR USE
G
Place the CBS™ High Security straw or the
CBS™ High Security tube holder;
G
Place the straw or tube on the holder and
gently push forward;
G
Automatic mode: As soon as the sensor detects
the straw or tube in the sealing position, the
sealing process starts automatically;
G
Manual mode: the straw or tube is sealed when
the operator presses the ‘WELD’ button on
touch screen.
TECHNICAL SPECIFICATIONS
Setup, maintenance and downtime time are
approximately 5 minutes per day.
Potentially contaminated parts of the system can
be cleaned with ethanol or non-corrosive decontamination fluids.
L x W x H: 363 x 276 x 152mm
Weight: 6 kg
23
Power supply: 110 or 220 V / 60 or 50 Hz
SYMS
Sealing system for CBS™ High Security straws
DESCRIPTION / USE
Sealing system: Cryo Bio System has developed
a thermal pulse sealing device for impermeable
sealing of CBS™ High Security straws in ionomeric resin.
The sealing device is electronically operated by
a push-button or foot pedal as desired.
G
Insert an inner identification rod, or outer
colored identification jacket;
G
Seal the other end of the straw;
G
Freeze according to a method appropriate for
the type of product.
TECHNICAL SPECIFICATIONS
L x W x H: 230 x 220 x 170 mm
INSTRUCTIONS FOR USE
G
After the filling procedure with microaspirator,
gently remove the transparent or yellow filling
nozzle;
G
Once this free part of the CBS™ straw is filled
and sealed, there is no longer any risk of contamination;
Weight: 6 kg
Power supply: 110 or 220 V / 60 or 50 Hz
016399 SYMS sealer for CBS™ High Security straws
009848 Spare kit for SYMS sealer (Teflon strips, fuses...)
CE marked
Assisted Reproductive Technologies
Equipment
PACE
SEMI-AUTOMATIC FILLING AND SEALING SYSTEM FOR CBSTM HIGH SECURITY STRAWS
CE marked
DESCRIPTION / USE
PACE semi-automatic filling and sealing system for CBSTM High Security straws
018451 PACE 220 V / 50 Hz
018450 PACE 110 V / 60 Hz
A semi-automatic system for packaging of
biological samples in CBS™ High Security
straws.
PACE automatically fills and thermally seals both
ends of CBS™ 0.3 or 0.5 mL straws from an
open primary sample tube.
CBS™ PACE system is the ideal packaging
system for sperm banks or medium to large-size
collection protocols.
PACE uses CBS™ straws that are pre-identified
with bar codes and/or alphanumerical codes or
just before printing with SIDE.
Different blood or sperm products from the same
individual may be processed in a pre-defined
order with the same filling nozzle.
24
The PACE system consists of 2 parts:
G
the straw distribution and filling unit and
G
its control box.
PACE machine cycle steps:
G
Hopper feeding sample straws individually;
G
Manual positioning of primary sample tube and
filling tubing;
G
Straw filling by aspiration with built-in pumps;
G
Controlled thermal autogenic sealing at both
ends of straw;
G
Manual transfer of straws to their storageaddress (visotube and goblet).
The throughput of straws is fully dependent on
the protocol (e.g. number of straws filled per
primary tube and the number of different biological products).
Setup, maintenance and shutdown: 15 minutes
per day.
All potentially contaminated parts can be cleaned
with ethanol or non-corrosive decontamination
fluids.
The PACE system can be placed on the laboratory
bench or under laminar flow with sterile straws
and filling tubing and only needs power supply.
TECHNICAL SPECIFICATIONS
PACE
L x W x H: 380 x 300 x 370 mm
Weight: 25 kg
G
Control box
L x W x H: 410 x 365 x 155 mm
Weight: 14 kg
G
007454 Sterile CBS™ injection tubing (by 5 x 10)
50
018620 Sterile long blue CBS™ injection tubing (by 5 x 5)
25
Power supply: 110 or 220 V / 60 or 50 Hz
007451 Sterile CBS™ aspiration tubing (by 5 x 10)
50
A PACE can be configured for CBS™ 0.3 or
0.5 mL straws.
MAPI
AUTOMATIC FILLING, SEALING AND PRINTING SYSTEM FOR CBSTM HIGH SECURITY STRAWS
CE marked
DESCRIPTION / USE
An automatic system for packaging and identification of biological samples.
007218 MAPI automatic filling, sealing and printing system for CBSTM High Security straws
From an open primary sample tube, MAPI
automatically fills CBS™ 0.3 or 0.5 mL straws,
thermally seals both ends, prints bar codes and/or
alphanumerical identification on the jacket and
in-line validates the process by immediate bar
code reading.
The MAPI system consists of the filling robot and
its control box, a special ink-jet type printer and
a PC with the MAPI pilot software. The automate
is placed on a special laboratory bench at ergonomical height.
25
MAPI machine cycle steps:
G
Hopper feeding of straws;
G
Bar code reading or key board entry of primary
tube code;
G
Manual positioning of primary sample tube and
filling nozzle;
G
Controlled straw filling by aspiration with builtin pumps;
G
Controlled thermal autogenic sealing at both
ends of straw;
G
Ink-jet printing of tamper-proof bar code and
corresponding text of up to 10 digits and
control key (code 128);
G
Reading of straw bar code for validation;
007454 Sterile CBS™ injection tubing (by 5 x 10)
50
G
Selection of validated straws;
018620 Sterile long blue CBS™ injection tubing (by 5 x 5)
25
G
Manual transfer of straws to their storage
address (visotube and goblet).
007451 Sterile CBS™ aspiration tubing (by 5 x 10)
50
The throughput of straws is fully dependent on
the protocol (e.g., number of straws filled per
primary tube and number of different biological
products).
Setup, maintenance and shutdown: 15 minutes
per day.
All potentially contaminated parts can be cleaned
with ethanol or non-corrosive decontamination
fluids.
The ink-jet printing system requires active evacuation of its solvent vapors.
MAPI pilot software
The MAPI pilot software is installed on the PC
provided with the system to pilot the machine
cycle of the MAPI system: distribution, filling,
sealing, printing and re-reading of straws. The
code to be printed on the straw can be either a
bar code read from the primary tube, keyboard
entered or programmed fixed text.
TECHNICAL SPECIFICATIONS
G MAPI
L x W x H: 760 x 260 x 470 mm
Weight: 35 kg
G Support table
L x W x H: 1200 x 600 x 900 mm
Height with machine: 1260 mm
Weight with machine: 102 kg
G Control box
L x W x H: 430 x 380 x 190 mm
Weight: 14 kg
G Printer Domino control box
L x W x H: 650 x 450 x 375 mm
Weight: 20 kg
Suction unit for DOMINO connectable to an
extractor duct, not included (diameter 80 mm)
Power supply: 110 or 220 V / 60 or 50 Hz
Assisted Reproductive Technologies
Equipment
Controlled rate freezers
016225 Planer Freezer Kryo 360-1.7
26
FROM PLANER
DESCRIPTION / USE
DESCRIPTION / USE
Kryo 360-1.7 / Kyro 360-3.3
Kryo 560-16
Fully featured biological freezer specifically
designed for cryopreservation of sperm, oocytes,
ovarian tissue and human embryos, in straws or
ampoules.
Fully featured biological freezer specifically
designed for cryopreservation of bone marrow,
stem cells, skin, cord blood, sperm, oocytes,
ovarian tissue and human embryos, in straws,
ampoules or bags.
G
Chamber volume: 1.7 or 3.3 liters
G
CBS™ sperm straw capacity: 45 or 90
G
CBS™ embryo straw capacity: 45
G
Chamber volume: 16 liters
Controlled range: +40°C to -180°C
G
CBS™ straw capacity: 456
G
Cooling rates: -0.01 to -50°C / min
G
G
Controlled heating rates: 0.01 to 10°C / min
Capacity: 11 x 250 / 500 ml bags, vertically in
chamber
G
Horizontal or vertical freezing
G
Controlled range: +30°C to -180°C
G
Cooling rates: -0.01 to -50°C / min
G
Controlled heating rates: 0.01 to 10°C / min
System pump: LNP4-C
G
System controller: MRV
G
System Dewar: LAB 20 or 30
G
System pump: LNP4-C
G
PC software: Delta T
G
System Dewar: LAB 30
G
PC software: Delta T
G
G
016130 Planer Freezer Kryo 360-3.3
G
System controller: MRV in 15 positions, radially
located
12-month warranty.
12-month warranty.
INDICATIONS
G
018134 Planer Freezer Kryo 560-16
Controller displays demand, sample and
chamber temperatures, programme stage and
current temperature graphic
G
Menu driven controller, simple to program and
operate
G
Horizontal or vertical operation
G
Compact design
G
Standard operating features:
- Start above ambient
- Controlled heating
INDICATIONS
G
Controller displays demand, sample and
chamber temperatures, programme stage and
current temperature graphic
G
Menu driven controller, simple to program and
operate
G
Protocol stage trigger on sample or chamber
temperature or time
G
Unique forced laminar flow system ensures
efficient, even cooling
G
Standard operating features:
- Data Printing (integral printer)
- Start above ambient
- Comms port for PC connection
- Controlled heating
- Fast cooling rates
- Data printing (integral printer)
- Multiple safety features
- Comms port for PC connection
- Fast cooling rates
- Multiple safety features
Coming soon!
CBS™ Co-XStraw™
The new generation of fully colored CBS™ High Security 0.3 & 0.5 mL straws
27
CBS™ High Security tube
A revolutionary High Security 1.2 mL sterile
container for long term storage of sperm and
ovarian tissue in liquid nitrogen. Safe sealed
as CBS™ High Security straws.
Cryo Bio System Netherlands
Amperestraat 4
8912 AD Leeuwarden
Netherlands
Tel.: +31 58 2123279
Fax: +31 58 2129813
Cryo Bio System Italy
Via Stefano Merli
29122 Piacenza
Italy
Tel.: +39 0523 36 00 10
Fax: +39 0523 38 07 23
Cryo Bio System India
Plot No 750, Phase V, Udyog Vihar
Gurgaon 122016 (Haryana)
India
Tel.: +91 124 400 44 30/31/32
Fax: +91 124 400 21 72
Cryo Bio System U.S.A.
11725 95th Avenue North
Maple Grove, MN 55369
USA
Tel.: +1 763 488 1881
Fax : +1 763 488 1888
Toll free within the USA: 1 800 DIAL IMV
(1 800 342 5468)
Cryo Bio System China
Room 2402, Beijing Anzhen Plaza
N°2 Andingmenwai Street
Dongcheng District, Beijing
China
Tel.: +86 10 64481509
Fax: +86 10 64282447
www.cryobiosystem.com
e-mail: [email protected]
Crédit photo : P.-Y. Le Meur – 06/2011
Cryo Bio System France
Groupe IMV Technologies
ZI n°1 Est
61300 L’Aigle
France
Tel.: +33 (0)233 346 464
Fax: +33 (0)233 341 198
Was this manual useful for you? yes no
Thank you for your participation!

* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project

Download PDF

advertisement