User Manual of AgileCycler

User Manual of AgileCycler
Note:
The Infinigen Biotech Inc. reserves all rights to modify this manual at
any time without notice.
Please read this manual carefully before operating Thermal Cycler !
Document Version: Jan, 2013, Version 1.2
AgileCycler
IMPORTANT INFORMATION
1. CAUTION
Note:
The column contains important information, please read it carefully. Failure to follow
can result in damage or malfunction of the Thermal Cycler.
Warning!
This symbol identifies certain cautions to be taken during operation/procedure.
Failure to follow the requirements may result in personal injury.
2. SAFETY PRECAUTION
The following safety precautions should be taken during operation, maintenance, or repair of the
Thermal Cycler, to prevent any reduction on safety and affection on operation of the Thermal Cycler
The Infinigen Biotech Inc. shall not be in any way responsible for the consequences resulted from
buyer’s neglect to the following requirements.
Note:
The Thermal Cycler is only intended for indoor use and to operation under
2,000-meter elevation.
a)
Do not attempt to open the case of machine or touch any parts inside the machine with
any tools.
b)
Grounding
A.C. power needed to be grounded to prevent any electric shock. Never let the third ground pin
hanging loose. If the 3-pin plug is not applicable, it is recommended to consult electrician for
appropriate power connection.
c)
Keep Away from Electric Circuits
Opening Thermal Cycler is strictly prohibited. Any component changing or parameter adjusting
inside Thermal Cycler should only be done by certificated professional personnel. Do not change
any element while the power is still on.
d)
A.C. Power
Before turning the power on, always check whether power source matches the required voltage
~100-240V and power output specification, i.e. 600W.
e)
Power Cord
Only use the power cord issued by manufacture for Thermal Cycler. Don’t not attempt to repair any
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AgileCycler
damaged power line, a damaged power cord must be replaced. The power cord should be kept free
from heavy objects during operation. The power cord should also kept away from tripping up
people.
f)
Connection
Hold the 3-pin plug and insert thoroughly to power socket to ensure good contact. When
disconnecting, pull the plug not the line.
g)
Operating Environment
The Thermal Cycler should be placed in a low moisture, dust free, and good ventilation environment
where no caustic gas or powerful magnetic interference exist. In addition, Thermal Cycler should be
kept distant from wet environment, such as pools and water pipes.
Never cover or obstruct the air vents of the Thermal Cycler, which are designed for ventilation and
to prevent the device’s interior from overheating. When a single device is running, minimum 30cm
distance should be kept between air vents and the nearest object. When 2 or more devices are
running simultaneously, a minimum 60 cm distance is needed in between. Do not place the device
on a soft surface, which may block the ventilation on the bottom of device.
Hot environment may degrade or failure on of the Thermal Cycler. Therefore, the device should be
protected against any kind of heat sources, such as sunlight, ovens, or central heating equipment.
When Thermal Cycler is to be in idol for long period of time, it is recommended to disconnect the
power and cover up the device to prevent dust from entering.
Note:
Immediately disconnect the power and contact the distributor or consult certified
mechanic for assistance when any of the following occurs.

Liquid gets into device;

The device is sprinkled or drenched;

The device malfunctions producing abnormal sound or odor;

The device falls down to the floor or outer shell is damaged;

Significant changes in the device’s performance.
3. Protection Degree: IP 20
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AgileCycler
4. Warning Sign
Icon
Meaning
High temperature warning:
Areas labeled with this warning signs and those described in the manual. Do not
touch to avoid getting burned.
Electric shocks Warning:
Operate strictly in accordance to the to avoid electric shock accidents!
Attention:
Important message. Read the message carefully. Failure to do so may cause
machine malfunction or damage to the machine.
WARRANTY AND SERVICE INFORMATION
1. Warranty
The Thermal Cycler is warranted for one month starting from date of ship out. Under this warranty
The Infinigen Biotech Inc. is obligated to exchange the Thermal Cycler with any defects result in
material and manufacture.
The Thermal Cycler is also warranted for thirteen months starting from date of ship out. Under this
warranty The Infinigen Biotech Inc.’s obligation shall be limited to repair or exchange (at the
Infinigen Biotech Inc.’s option) of the Thermal Cycler proven to be defective as described herein.
The buyer is responsible for freight to the maintenance shop designated by the Infinigen Biotech Inc.
as well as the freight cost for all warranty claims. The Infinigen Biotech Inc. will cover the
transportation expenses of the freight from maintenance shop back to the buyer (only applicable to
domestic freight).
After the warranty is due, the Infinigen Biotech Inc. reserves the right to charge cost for
maintenance accordingly.
2. Warranty Terms
The above warranty is not applicable to defective devices due to improper usage, abnormal
operating conditions, improper application, and unauthorized maintenance or alteration.
The Infinigen Biotech Inc. provides no extension warranties other than those described herein. Any
descriptions in sales promotion shall not create an extension warranty.
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AgileCycler
Note:
Check items included in the package in accordance to the checklist at receive.
Contact the distributor when finding any missing or damaged items in the package.
Buyers are requested to fill out the check form and send a photocopy (or via fax)
back to the distributor after finishing item checking. The returned form will be filed
into Infinigen Biotech Inc.’s archives and maintenance record.
Please store the package and packing materials in a safe place for future uses. The
above warranty does not extend to goods damaged due to improper package.
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AgileCycler
CONTENTS
CHAPTER 1 INSTALLATIONS ............................................................................ - 1 1.
The AgileCycler Color Touch Thermal Cycler package includes: ................. - 1 2.
Normal Operating Conditions ..................................................................... - 1 -
3.
Transportation and Storage ........................................................................ - 1 -
4.
Parts and Functions ..................................................................................... - 1 -
5.
Module changing ......................................................................................... - 4 -
6.
Features ....................................................................................................... - 6 -
7.
Specifications............................................................................................... - 7 -
CHAPTER 2 OPERATION GUIDE ...................................................................... - 9 1.
Power-on Procedure ................................................................................... - 9 1.1.
Boot ............................................................................................................. - 9 -
1.2.
Warm-up ................................................................................................... - 10 -
2.
Menu structure ......................................................................................... - 11 -
3.
File ............................................................................................................. - 12 -
3.1.
Create and edit file .................................................................................... - 12 -
3.2.
Print file ..................................................................................................... - 17 -
3.3.
Delete file .................................................................................................. - 17 -
3.4.
Copy file ..................................................................................................... - 17 -
3.5.
Rename file................................................................................................ - 18 -
3.6.
Run file....................................................................................................... - 18 -
4.
Setting ....................................................................................................... - 19 -
4.1.
Run Setting ................................................................................................ - 20 -
4.2.
System Setting ........................................................................................... - 21 -
5.
Tools .......................................................................................................... - 22 -
5.1.
Gradient calculator .................................................................................... - 22 -
5.2.
Logs............................................................................................................ - 23 -
5.3.
Local information ...................................................................................... - 23 -
6.
Incubate..................................................................................................... - 24 -
AgileCycler
CHAPTER 3 MAINTENANCE & TROUBELSHOOTING.................................... - 25 1.
Maintenance ............................................................................................. - 25 1.1.
Cleaning ..................................................................................................... - 25 -
1.2.
Replace fuse .............................................................................................. - 25 -
2.
Troubleshooting ........................................................................................ - 25 -
2.1.
Dissatisfactory Results............................................................................... - 25 -
2.2.
Self-test hardware and self-diagnosis software ........................................ - 26 -
3.
Notices ....................................................................................................... - 26 -
3.1.
Power ........................................................................................................ - 26 -
3.2.
LCD touch screen ....................................................................................... - 26 -
3.3.
Notice on cleaning ..................................................................................... - 27 -
4.
Error message & solution .......................................................................... - 27 -
5.
Cause of the abnormal phenomenon & solution ...................................... - 28 -
AgileCycler
CHAPTER 1 INSTALLATIONS
1. The AgileCycler Color Touch Thermal Cycler package
includes:




AgileCycler Color Touch Thermal Cycler
Power Cord
2 pieces of 8A 250V fuses
Instruction manual
2. Normal Operating Conditions
Ambient temperature: 10°C~30°C
Relative humidity:
≤70%
Power source:
85-264V, 50-60Hz, 600W
Note:
Please check if the above operating conditions are met before turning the power
on. Pay special attention to power line’s grounding.
3. Transportation and Storage
Ambient temperature: -20°C~+55°C
Relative humidity:
≤80%
4. Parts and Functions
Status LED
Reaction
module bay
LCD display
Air vents
USB A port
Figure 1. Frontal view of Thermal Cycler.
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● Reaction module bay — holds reaction module
● Air vents — allows air circulation
● Status LED — indicates status of reaction module
● LCD display — displays operating status
● USB A port — 2 USB ports, connection for computer mouse and USB devices
Connector
Locking screw
Fuses
Power switch
USB B port
Power input
Test port
Ethernet port
Figure 2. Rear view of thermal cycler.
● Connector — connection between host machine and reaction module
● Locking screw — locks reaction module
● Test port — for service testing only
● Ethernet port — connection to computer
● USB B port — for system debug
Note:
1 The fuse specifications are: 250V 8A, φ5×20mm. Use only the fuse that meets the
above specifications. For certificated fuses, please contact distributor.
2 For exchanging block, please consult your distributors.
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Lid knob
Lid pull
Connector
Cooling fins
Figure 3. The lid and cooling fins of 96-well reaction module
● Lid knob — to seal Lid
● Lid pull — to open and close lid
● Connector — connection between host machine and reaction module
Inner Lid
Reaction block
Figure 4. Opening view of a 96-well reaction module
● Reaction block — holds reaction vessels, including tubes and microplates
● Inner lid — applies heat and force to the reaction vessel lids (caps or tape) to
produce consistent and successful reactions. Heating the inner lid prevents
condensation, while applying force seals the reaction to prevent evaporation.
Warning!
After a run, the heated inner lid can remain hot. Use caution when opening and
close the lid.
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5. Module changing
Note:
1 Make sure the power is off before module changing
2 Maintain module temperature as close as the ambient temperature.
Step 1 Open PCR lid and remove the 2 screws on the back of PCR by turning
counterclockwise
Screws
Step 2 Extract module by pulling the handle outwards.
Handle
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Step 3 Before being completely extracted, hold the module to prevent it from falling off.
Step 4 Insert new module as shown after removing its cover.
Step 5 Push new module inwards completely to insure a fixed connection between the
module and machine.
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Step 6 Tighten the 2 screws and close PCR lid after inserting new module.
6. Features
1. Each thermal block has 4 independent temperature control sensors and 8 peltier
heating units to ensure accurate and uniform temperature across block surface, and
provide users for replicate previous condition set-up.
2. 8” TFT color touch-screen with graphical display provides easy use for setting up and
monitoring.
3. The actual and target temperatures can be viewed and displayed graphically in real
time.
4. 30 thermal cyclers can be simultaneously linked to one PC via LAN, which eliminates
troubles of setting up and programming each thermal cycler.
5. Max. temperature gradient 30℃.
6. Applicable with the following 0.2ml tube models: non-skirted, semi and fully skirted
plated.
7. Compatible with devices such as Mouse and Keyboard and capable to transfer data
and perform software updates via USB Drive.
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7. Specifications
Catalogue Number
Sample Capacity
AC-96
AC-60
AC-48X2
AC-48N30
AC-384
96×0.2ml
60 x 0.5ml
2 x 48
x 0.2ml
48 x 0.2 ml +
30 x 0.5 ml
384well
Temperature Range
0~100℃
4~100℃
Maximum ramp rate
5℃/s
3.0℃/s
Average ramp rate
3℃/s
2.0℃/s
Uniformity
≤ ±0.2℃
≤ ±0.3℃
Accuracy
≤ ±0.1℃
≤ ±0.2℃
0.1℃
0.1℃
Ramping Rate Adjustable
0.1~5℃
0.1~3.0℃
Gradient Uniformity
≤±0.2℃
≤±0.3℃
Gradient Accuracy
≤±0.2℃
≤±0.3℃
30~100℃
30~100℃
1~30℃
1~24℃
Display Resolution
Gradient Temp. Range
Gradient Span
Temperature Control
Block\Tube
Hot Lid Temperature
30~110℃
Yes
Over-temperature cut-out
Number of Programs
10000 +(USB FLASH)+intuitive “drag and drop” program creation.
End of program alarm
Yes(can be disabled)
Max. No. of Step
30
Max. No. of Cycle of each
step
99
Time
Increment/Decrement
1 Sec ~ 9 Min 59 Sec
Temp.
Increment/Decrement
0.1~9.9℃
Auto-start on power failure
Yes
Connection to PC control
software
Yes
Gradient calculator
Yes
Password protection
Yes
User level
Yes
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Hold at 4℃
Yes
Printing
Yes
Wireless blue tooth
Yes
LAN to computer
Yes
LCD
8 inch,800×600 pixels, TFT
Real time temperature
sensor feedback
Yes
Communication
USB2.0 , LAN
Noise emission
Very low
Dimensions
38cm×24cm×26cm (L×W×H)
Weight
Power Supply
8.5kg
85~264VAC , 47~63Hz , 600 W
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AgileCycler
CHAPTER 2 OPERATION GUIDE
Warning!
Please turn off the power immediately and contact your distributor when any
abnormal sound or display appears after power-on, or when failure alarm and
display are found during device’s self-testing process
Note:
When sample number is less than available conical holes, sample tubes should be
evenly placed over the block to ensure the stable coverage and even temperature
fluctuation distribution in blocks.
Note:
In order to guarantee that the heated lid is seamlessly sealed with the sample tubes
and firm contacts between tubes and conical holes, please apply light pressure onto
the lid’s flanks when closing.
1. Power-on Procedure
1.1. Boot
Connect power cord to the back of instrument then plug into power source and switch to
"-". When instrument is on, a buzzer should be heard and Boot screen (Figure 5) will
appear on LCD screen. The operating system then goes to Self-Test screen (Figure 6).
During this phase, the LCD screen will display instrument model and company LOGO.
Home screen (Figure 7) will be shown when starting up phase is completed.
Figure 5. boot screen
Figure 6. self-test screen
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AgileCycler
Figure 7. home screen
1.2. Warm-up
In standby modes the instrument will pre-heat the Block and Hot Lid. Please refer to
(Run Setting) for setting instructions of Hot Lid work mode. The default Hot Lid
pre-heating procedure is carried out once the power is on, and the default temperature is
105 ° C.
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AgileCycler
2. Menu structure
Home
File
New
Edit
Run
Print
Options
Copy
Paste
Delete
Rename
Back
Setting
Run setting
Hot lid Temp
Hot lid work mode
Lid auto closed Temp
Temp control
System setting
Time/Date
Language
Sound
Tools
Gradient calculator
Logs
Local information
Incubate
Help
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AgileCycler
3. File
File is composed by Temperature Step and Cycle Step. Each File can contain up to 30
Steps. Temperature Step contains Temperature, Time, Gradient, Ramp Rate,
Temperature Increment and Time Extend. The maximum cycle in Cycle Step is 99.
3.1. Create and edit file
In the Home screen (Figure 7), touch “File” button to enter File Library screen (Figure 8).
Figure 8. File Library
1). Change Target Temperature and Hold Time
 From a new file
Touch “New File” button in the File Library screen (Fig. 8) to create new file (Fig. 9).
Figure 9. Create a new file
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AgileCycler
In the New File screen, touch “+Temp” button to add a Temperature Step, touch
“+Cycle” button to add Cycle Step (GOTO step), use the pop-up numeric keypad to
enter a new value for the target temperature or hold time.
 From an existing file
Select a file in the File Library screen (Fig. 8), touch “Edit” button to modify selected
files.
Figure 10. Edit an old file
In the Edit File screen (Fig. 10), select a desired step by touching anywhere in the
step. Change a time or temperature by touching the desired field and entering a value
using the pop-up numeric keypad. To enter an infinite hold, fill time field with 00:00.
2). Edit Step
Select a desired step, touch Options button to enter Step Options screen (Figure 11).
Parameters can be edited by selecting among Gradient, Ramp Rate, Temperature
Increment or Time Extend. Table 1 lists the parameters for temperature and gradient
steps with the limits of those parameters.
After entering Gradient values the gradient distribution table (Figure 11) will be shown at
bottom of the screen. Touch “OK” to save changes and return Edit File screen (Figure
12).
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AgileCycler
Figure 11. Set the Gradient
Figure 12. Gradient on Step 3
Table 1. List of parameters for temperature and gradient steps
Parameter
Ranges
Description
Temperature
Temperature in ℃: The target
Instructs the thermal cycler to
temperature between 0.0 and 100.0℃
ramp to the target temperature.
in tenths of a drgree
Hold time: The hold tine between 1
sec and 59min 59 sec in the format of
min : sec. To enter an infinite hold,
touch 0 button, the symbol ∞ (infinite)
will come out.
Gradient range
Lower: The lower temperature in the
Instructs the thermal cycler to
gradient. Enter a number between 30.0
ramp to the target temperature
and 99.9℃ in tenths of a degree.
gradient across the block and
hold that temperature gradient
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AgileCycler
Upper: The upper temperature in the
for the specified amount of time.
gradient. The max. temperature is
100.0℃. Enter a temperature within
30.0℃ of the lower temperature.
Hold time: The hold tine between 1
sec and 59min 59 sec in the format of
min : sec. To enter an infinite hold,
touch 0 button, the symbol ∞ (infinite)
will come out.
Increment
A temperature from -9.9℃ to 9.9℃
Applies only to a temperature
per cycle in tenths of a degree.
step. Instructs the thermal
cycler to increment (change)
the target temperature of a step
with each cycle, where a
positive number increases the
temperature and a negative
number decreases the
temperature.
Ramp rate
A number from 0.1 to 5℃ per sec
Applies only to a temperature
step. Instructs the thermal
cycler to ramp tp the target
temperature at the specified
ramp rate in that step.
Time
A time from 1 sec to 59 min 59 sec
Instructs the thermal cycler to
hold the temperature for the
specified amount of time.
Extend
A time from -9 min 59 sec to 9min 59
Applies to both temperature
sec per cycle.
and gradient steps. Instructs the
thermal cycler to extend the
hold time with each cycle. A
positive number increases the
hold time and a negative
number decreases the hold
time.
3). Insert a Step
Insert a step if a new temperature, Cycle (GOTO), or gradient step is needed. Follow
these instructions to insert a step to the right of a preexisting step.
I. Touch a step to the left where the new step will be inserted.
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II. Touch the “+Temp” button to insert a step or the “+Cycle” button to insert a Cycle
(GOTO)
III. Touch the time or temperature field to edit the parameter in the new step, or touch the
step or times field to edit the parameter in the new Cycle (GOTO).
4). Delete a Step
To permanently remove a step from file.
I. Select the step to be deleted.
II. Touch “Delete” button to delete the selected step.
5). Remove or add a temperature gradient
Select a step in “Edit file” screen and touch the “Options” button to enter Step Options
screen. Touch “Cancel” button to remove a temperature gradient, or fill the Step Options
and touch “OK” button to add a temperature gradient.
6). Save file
When editing is completed, touch “Done” to enter Save File screen (Figure 13) and touch
“Browse" button to set file saving path. Then touch “File Name” button to name file and
finally touch “Save” button to save file and return File Library screen (Figure 14)
Figure 13. Name file and select the path
.
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AgileCycler
Figure 14. New file in the library.
3.2. Print file
Touch “Print” button to print selected file. The Instrument will connect to Bluetooth printer
automatically when the printer is on.
3.3. Delete file
Touch “Options” button in the File Library screen (Figure 8) to select the file to delete
and the query dialog window will pop up when touching “Delete” button. When confirm a
deleting, touch “Yes” button (Figure 15). To prevent any files being mistakenly deleted,
only one delete per time is allowed.
Figure 15. Delete a file
3.4. Copy file
Touch “Options” button in the File Library screen (Figure 8) to select files and touch “copy”
button.
Then enter the file path and touch “Paste” button to finish copy.
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AgileCycler
3.5. Rename file
Touch “Options” button in the File Library screen (Figure 8) and select file. Then touch
“Rename” button and enter new file name in the dialog window to rename files.
3.6. Run file
Select the file needed in the File Library screen (Figure 8) and touch “Run” button to
enter the Run File Setup screen (Figure 16).
Figure 16. Run file setup
Then select the Block and enter volume (not necessary to be in block temperature
control mode). Touch Start, enter the File Running screen (Figure 17).
Figure 17. File running.
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AgileCycler
1)
Parameters
 Start Time — system time when file starts to run.
 Remain Time — remaining time for the experiment.
 Lid Temp — current hot lid temperature.
 Step — current step.
 Step Time — current step time.
 Block Temp — current block temperature.
2)
Pause
Touch “Pause” button to pause running file and touch ”Resume” button to restore
running.
3)
Stop
Touch “Stop” button to stop a running file.
4)
Skip
Touch” Skip” button to jump to next step.
5)
Go to other screen
Touch “Home” button to return Home screen.
Touch “View” button to enter the Navigation screen (Figure19).
Figure 18. The navigation screen.
4. Setting
Touch “Setting” button in the Home screen to enter the Setting screen (Figure 19).
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AgileCycler
Figure 19. Setting menu
4.1. Run Setting
Select the “Run Setting” button in the Setting screen (Figure 19) to enter the Run
Setting screen (Figure 20).
Figure 20. Run parameter setting
1)
Hot lid temperature setting
Hot lid temperature setting range is 30-110℃ and system default temperature is
105℃.
2)
Hot lid work mode setting
In total are 4 working modes and default mode is that the hot lid will be on when power is
on.
3)
Lid heater auto shutoff temperature
The range of lid heater auto shutoff Temp is 0-100℃ and system default is 25℃.
When the block is running an infinite hold at a temperature which is lower than the
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AgileCycler
specified auto shutoff temperature, the lid heater will turn off and the lid temperature
will stay at block temperature.
Note:
4)
Lid Temperature
The adjustable heated lid of AgileCycler allows user to control the lid
temperature and force. When AgileCycler is running, the heated lid maintains
the temperature specified by the file being run. Without a heated lid, water
can be lost from the reagents to condensation, concentrating the reactants in
the tube or plate. However, if the temperature of heated lid is too high, the
sample temperature might rise above the target temperature. Therefore, pay
attention to the setting of lid temperature could make your experiment result
more reproducible.
Temp control mode
The temp control mode contains “block control mode” and “tube control mode”. The “block
control mode” is suitable for performing normal PCR and the “tube control mode” is for
experiments that require higher environment conditions. The system default is “block
control mode”.
4.2. System Setting
Select “System Setting” button in the Setting screen (Figure 19) to enter the System
Setting screen (Figure 21).
Figure 21. System parameter setting.
1)
Time and date
System time and date setting
2)
Language
System supports English only.
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AgileCycler
3)
Sounds
 Keyboard sound — sound when touch button.
 Alarm sound — sound when system error occurs.
 File end sound — sound when File running is complete.
 Temp sound — sound when the target temperature is reached.
5. Tools
Touch “Tool” button in the Home screen to enter Tools screen (Figure23).
Figure 22. Tools menu
5.1. Gradient calculator
The temperature value for each column is shown after entering the block target
temperature and gradient range (Figure 23).
Figure 23. Gradient calculator
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AgileCycler
5.2. Logs
System logs name, running starting time, and times of test for each file. Maximum 1000
logs can be recorded and the system will remove older logs once the capacity is excess
(Figure 24).
Figure 25. Local information.
5.3. Local information
Show the IP address and block type (Figure 25).
Figure 24. Run logs.
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AgileCycler
6. Incubate
Touch “Incubate” button in the Home screen to enter Incubate Setup screen (Figure 26).
Figure 26. Incubate setup
Incubation function maintains steady block temperature for unlimited period. First, select
the desired blocks in the Incubate Setup screen (Figure 26) and enter block temperature,
then touch “Start” button to enter Incubate Running screen (Figure 27).
Figure 27. Incubate running
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AgileCycler
CHAPTER 3 MAINTENANCE & TROUBELSHOOTING
1. Maintenance
1.1. Cleaning
 Clean the holes on reaction block with neutral soap solution (Do not use any solvent
contains alkali, alcohol or organic solution)
 Spaces near the left, right air vents and under the machine should be always kept free.
It is important to clean the dust around air vents regularly.
 Clean reaction block regularly and remove any residue inside cavities to prevent any
affects to the temperature control. (soft cloth advised).
 Soft cloth can also be used to scrub the dirty spots of the machine surface.
Warning!
Turn off the power during cleaning.
Corrosive solvent is not allowed for cleaning the surface.
1.2. Replace fuse
The machine has two fuses.
Once damaged, please replace damaged fuses with following
steps.
1)
Turn off the machine and unplug power source.
2)
Unscrew the fuse box with flat screwdriver and replace the damaged fuses with new 8A
250V fuses. After replacement, screw back in the fuses box.
Attention: please contact your local distributors when encountering any problem
replacing fuses.
2. Troubleshooting
2.1. Dissatisfactory Results
Biological, programmatic and hardware problems may contribute to any unsatisfied results
of experiment.
In order to distinguish the hardware problems from other possible
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AgileCycler
problems, the machine is equipped with built-in self-test hardware and self-diagnosis
software.
Below is the detailed description.
According to the experiences, most
problems are related to biological and programmatic factors.
1)
Incorrect or insufficient reactants.
2)
The temperature is too high or too low.
90-95℃ for 40 seconds.
It is suggested to set the temp. within range of
The duration can be adjusted in accordance with reaction
volume.
3)
“Annealing” temperature is too high or too low.
It is better to between 55-70 ° C and 20
to 30 chains.
4)
The reactant concentration is too high or too low.
5)
Lack of special treatment during preparations.
6)
Time and temperature programming are not appropriate.
7)
Sample temperature is slightly too low and the block temperature is slightly too high.
8)
Check whether the PCR tubes are well placed. Smearing little mineral oil on the surface of
holes will increase the thermal conductivity.
2.2. Self-test hardware and self-diagnosis software
The machine will run the self-test program when booting.
3. Notices
3.1. Power
1)
No special requirements for the power source. Any AC power source within range of
85V~264V is applicable.
However, to prevent from causing any damage to the machine,
it is better to apply low voltage fluctuation power sources, otherwise, power source
regulator should be applied.
2)
It is strictly prohibited to terminate a running experiment by cutting off the power. Doing so
will be very harmful to the machine.
3.2. LCD touch screen
Do not UV sterilize the LCD screen to prevent causing any damage.
scratch to the LCD screen while using.
- 26 -
Avoid any bump or
AgileCycler
3.3. Notice on cleaning
Please avoid any liquid getting inside the machine while cleaning reaction block. Due to the
possible usage of radioactive substance during experiment, please carefully handle cleaning.
Please do not operate and use the machine in moisture or hot environment.
Attention : Please do read the Notices carefully to prevent causing any damage to
the machine.
4. Error message & solution
No.
error message
Cause and solution
1
File name empty
Null character can not be used for file name
2
Same file name, Please re-name
Do not support multiple files with same file
names
3
Module sensor 1 short circuit
Hardware problem, contact certified mechanic
for repair
4
Module sensor 1 open circuit
Hardware problem, contact certified mechanic
for repair
5
Module sensor 2 short circuit
Hardware problem, contact certified mechanic
for repair
6
Module sensor 2 open circuit
Hardware problem, contact certified mechanic
for repair
7
Module sensor 3 short circuit
Hardware problem, contact certified mechanic
for repair
8
Module sensor 3 open circuit
Hardware problem, contact certified mechanic
for repair
9
Module sensor 4 short circuit
Hardware problem, contact certified mechanic
for repair
10
Module sensor 4 open circuit
Hardware problem, contact certified mechanic
for repair
11
Radiator sensor short circuit
Hardware problem, contact certified mechanic
for repair
12
Radiator sensor open circuit
Hardware problem, contact certified mechanic
for repair
13
lid sensor short circuit
Hardware problem, contact certified mechanic
for repair
14
lid sensor open circuit
Hardware problem, contact certified mechanic
for repair
15
Power output short circuit
Hardware problem, contact certified mechanic
- 27 -
AgileCycler
for repair
16
Block temperature too high
Air flow vent is blocked or circuit problem or
need repair
17
Block temperature too low
Environmental temperature is too low or circuit
problem or need repair
18
Heat Sink temperature too high
Air flow vent is blocked or fan problem or need
repair
19
Heat Sink temperature too low
Environmental temperature is too low or circuit
problem or need repair
20
Hot Lid temperature too high
circuit problem or need repair
5. Cause of the abnormal phenomenon & solution
No.
1
Description of problem
No display after booting
Cause and solution
Check whether the plug is inserted correctly and
check the power output.
Please turn off the
machine and disconnect the plug to check the
fuse.
2
3
4
After power on, the machine start
The power was shut down before the previous
running the program
program is completed
The fan runs fast some time, slows
Normal, The fan is for cooling the heating pump
down some time
not for reaching the targeted temperature
Slight pat or squeak sound while
Normal. The sounds are from the power switch
machine is running
mechanism when quick cooling or heating is
needed.
5
Slow increasing or decreasing temp.
Check the settings of heating and cooling rate
and check whether the fan is operating normally
6
Wrong display appeared one LCD
Caused by electrostatic pulse or power surge,
screen
please turn off and then turn on the machine.
It will not affect program running.
Please contact the local distributor in time if the above problems can’t be solved.
- 28 -
FUSE
N
PE
N
PE
L
L
L
N
PE
DC1+
DC1+
AC Input
24V OUT
AC/DC2
SW ITCH1
FUSE 2
FILTE R1
DC Output
SO CK ET1
FUSE
FUSE
AC Input
AC 100-240,50/60Hz
FUSE
FUSE 1
NO1
NO2
HOTLID
BO AR D3
DC Output
DC2+
DC2+
DC2+
DC2+
DC2DC2DC2DC2-
5V OUT
C1
C2
J1-1_LID_SENSOR1
J1-2_LID_SENSOR2
L
N
PE
J2-1_LID_POWER1
J2-2_LID_POWER2
J9_FFC64P_LINK
J10-1_LID_SENSOR1
J10-2_LID_SENSOR2
AC/DC1
L
PE
N
- 29 J9-1_+5V
J5-2_GND
J16-1_LID_POWER1
J16-2_LID_POWER2
J5-1_+24V
BLOCK
BO AR D4
DRIVE
BO AR D1
J5-2_GND
J6-1_+24V
J6-2_GND
J7-1_+24V
J7-2_GND
J8-1_+24V
J8-2_GND
J2-1_LID_B+
J2-2_LID_B-
J4-1_LID_B+
J4-2_LID_B-
LINK
J2-5_TE_B_B+
J2-6_TE_B_BJ2-7_TE_B_F+
J2-3
J2-8_TE_B_FJ2-4
BO AR D5
J4-5_TE_B_B+
J4-6_TE_B_BJ4-7_TE_B_F+
J4-3
J4-8_TE_B_FJ4-4
J3-1_TE_A_F+
J3-2_TE_A_FJ3-3_TE_B_F+
J3-4_TE_B_F-
-
+
8INCH 800*600
LCD1
FAN1
J5-1_TE_A_F+
J5-2_TE_A_FJ5-3_TE_B_F+
J5-4_TE_B_FJ2_FFC64P_BLOCK
J5-7_LID_A+
J5-8_LID_AJ5-5
J3_FFC26P_CPU
J5-6
J3-7_LID_A+
J3-8_LID_AJ3-5
J3-6
J4-1_FAN+
J1_FFC26P_CPU
J4-2_FAN-
J4_FFC4P_CPU
J3_TEST
J2_RJ45
J1_FFC16P_ARM
BACK PORT
BO AR D8
J2_USB_A_PORT
J1_FFC12P_USB
USB
BO AR D7
J4_FFC50P_LCD
J3_FFC16P_BACKPORT
J2_FFC12P_USB
J1_FFC12P_CPU
ARM
BO AR D6
J6_FFC26P_LINK
J5_FFC26P_DRIVE
J4_FFC12P_ARM
J3_FFC10P_JATG
J2_FFC20P_TESTLCD
J1_FFC4P_BACKPORT
CPU
BO AR D2
AgileCycler
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