Living up to Life
Leitz Optilux
User Manual
Congratulations on purchasing the Leitz Optilux
Compound Microscope. This model’s exclusive
design features and full range of accessories
make it a truly versatile, high quality-instrument.
Though the reliability and robustness of Leica
microscopes is already practically proverbial,
they still require a certain degree of care and
attention. Therefore, we recommend that you
read this User Manual. It contains all the information you need regarding operation, safety
and maintenance. Simply observing a few
guidelines will ensure that even after years of
intensive use, your microscope will continue to
work as smoothly and reliably as on the very
first day.
We wish you the best of success in your work!
Leitz Optilux User Manual
Important Notes
Symbols Used in this User Manual
Important Safety Notes
Instructions for Use
Health Risks and Dangers of Use
Information for the Person Responsible for the Instrument
Accessories, Maintenance and Repair
Electrical Data and Ambient Conditions
Using the Leitz Optilux
Switching on the Microscope
Selecting an Objective
Preparing for Viewing
Adjusting the Eyecups
Oil Immersion Technique
Care of the Microscope
Maintenance instructions
General Maintenance
Assembling the Leitz Optilux
Installing the Monocular Tube
Removing and Inserting Objectives
Installing the Mirror (optional)
Installing the Polarizer Kit (optional)
Leitz Optilux User Manual
Important Notes
Leitz Optilux User Manual
Important Notes4
Symbols Used in this User Manual
Warning! Safety hazard!
This symbol indicates especially important information that is mandatory to
read and observe.
Failure to comply can cause the following:
OO Hazards to personnel.
Warning of hazardous electrical Voltage
This symbol indicates information that
must be read and observed.
Failure to comply can cause the following:
OO Hazards to personnel.
Important Information
This symbol indicates additional information or explanations that are intended
to provide clarity.
Instrument malfunctions and damage.
Instrument malfunctions and damage.
Leitz Optilux User Manual
Important Notes5
Important Safety Notes
Before installing, operating or using
the instrument, it is mandatory to read
this User Manual. In particular, please follow all
safety instructions.
The instruments and accessories
described in this User Manual have been
tested for safety and potential hazards.
Original Condition
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in this User Manual.
Leitz Optilux User Manual
Non-intended Use
Using the instrument in any way other
than that described in this User Manual
may result in personal injury or damage to
property. Doing so can impair the functions of
the protective measures provided.
"Safety Concept" Brochure
The "Safety Concept" brochure contains additional safety information regarding service
work, requirements and the handling of the
microscope, the electrical and other accessories
as well as general safety instructions.
The responsible Leica affiliate must be
consulted whenever the instrument is
altered, modified or used in conjunction with
non-Leica components that are outside of the
scope of this User Manual! Unauthorized alterations to the instrument or noncompliant use
shall void all rights to any warranty claims.
Important Notes6
Important Safety Notes (continued)
Legal Regulations
Adhere to general and local regulations relating to accident prevention and environmental
EC Declaration of Conformity
constructed based on the state of the art of
technology and are provided with an EC Declaration of Conformity.
Disconnecting from the Power Supply
Always position the Leitz Optilux so
that you can disconnect it from the
power supply at any time. The power cable
must remain accessible at all times, because
the power cable is intended as a power disconnect device.
install any other plug
(NEMA 5-15P) or unscrew any mechani-
cal components unless expressly instructed to
do so in the instructions.
Leitz Optilux User Manual
Important Notes7
Instructions for Use
This User Manual describes the Leitz Optilux
and includes important instructions related to
operating safety, maintenance and accessories.
Your Leitz Optilux includes an interactive
CD-ROM with all relevant User Manuals in
additional languages. Keep it in a safe place,
and readily accessible to the user. You can also
download User Manuals and updates from our
website at www.leica-microsystems.com.
Intended Use
The Leitz Optilux is an optical instrument for
improving the visibility of objects through
magnification and illumination. It is used for
Place of Use
The Leitz Optilux microscope may be used only
in closed rooms and must be placed on a solid
Avoid large temperature fluctuations, direct
sunlight and vibrations. These conditions
can distort measurements and micrographic
In warm and warm-damp climatic zones,
the individual components require
special care in order to prevent the build-up of
The Leitz Optilux can be used at +10 °C to
+40 °C. Protect the microscope from oil, chemicals and extreme humidity. If it is used outdoors,
the microscope must be protected from dust
and moisture. Never use electrical instruments
outdoors and place them at least 10 cm away
from the wall and from flammable substances.
Leitz Optilux User Manual
Important Notes8
Instructions for Use (continued)
Accessories from third-party Suppliers
You can combine individual system articles with
articles from external suppliers. Please read the
User Manual and the safety instructions from
the supplier.
Integration in third-party products
When installing Leica products into third-party
products, the manufacturer of the complete
system or its dealer is responsible for following all applicable safety instructions, laws and
Leitz Optilux User Manual
If at all possible, use the original packaging for
shipping or transporting individual modules.
In order to prevent damage from vibrations,
all moving parts that (according to the User
Manual) can be assembled and disassembled
by the customer should be packed separately.
Disposal must comply with locally applicable
laws and regulations.
Important Notes9
Health Risks and Dangers of Use
Health Risks
Workplaces with microscopes facilitate and improve the viewing task, but
they also impose high demands on the eyes
and hand muscles of the user. Depending on
the duration of uninterrupted work at such a
workplace, impaired vision and problems with
the musculoskeletal system may occur. For this
reason, appropriate measures for reduction of
the workload must be taken:
Optimum workplace layout
Frequent changes of activity
Thorough training of the personnel, giving
consideration to ergonomic and organizational aspects
The ergonomic optical design and construction
of the Leica microscopes are intended to reduce
the exertion of the user to a minimum.
Danger of Infection
Direct contact with eyepieces can be a
potential transmission path for bacterial
and viral infections of the eye.
The risk can be minimized by the use of
personal eyecups.
Dangers during Use
The Leitz Optilux may be connected to a
grounded socket only.
The Leitz Optilux may be used when it is in
proper working condition only.
LED Class 1 /LED 1 Laser Product
The illumination meets laser class 1 requirements in accordance with IEC 60825-1 "Safety
of Laser devices".
Caution: Hazardous radiation may occur if
controls, adjustment jigs, or procedures other
than those outlined in this User Manual are
Class 1
LED Product
Leitz Optilux User Manual
LED Class 1
Important Notes10
Information for the Person Responsible for the Instrument
Information for the Person Responsible for
the Instrument
OO Ensure that the Leitz Optilux is used only by
persons qualified to do so.
Ensure that this User Manual is always available at the place where the microscope is
in use.
Carry out regular inspections to make
certain that the authorized users are adhering to safety requirements.
When instructing new users, do so thoroughly and explain the meanings of the
warning signs and messages.
Allocate responsibilities for commissioning, operation and maintenance. Monitor
compliance with this.
Do not use the Leitz Optilux unless it is in
perfect condition.
Leitz Optilux User Manual
Inform your Leica representative or Leica
Microsystems (Schweiz) AG, Industry
Division, 9435 Heerbrugg, Switzerland,
immediately of any product defect that
could potentially cause injury or harm.
If you use accessories from other manufacturers with the microscope, make sure
that these manufacturers confirm that
the combination is safe to use. Follow the
instructions in the User Manual for those
Modifications to or service on the microscope may be carried out only by technicians who are explicitly authorized by Leica
to do so.
Only original Leica replacement parts may
be used in servicing the product.
After service work or technical modifications, the device must be readjusted in
accordance with our technical specifications.
If the unit is modified or serviced by unauthorized persons, is improperly maintained
(as long as maintenance was not carried
out by Leica Microsystems), or is handled
improperly, Leica Microsystems will not
accept any liability.
The building’s electrical wiring must
comply with the relevant national standard; in other words, we recommend using
a current-operated ELCB system (RCB).
Important Notes11
Accessories, Maintenance and Repair
Only the following accessories may be used
with the Leitz Optilux:
The Leica Microsystems accessories
described in this User Manual.
Other accessories, provided that these have
been expressly approved for use by Leica
Microsystems with regard to safety technology.
The Leitz Optilux microscope is basically
maintenance-free. To ensure that it always
operates safely and reliably, we recommend
that you take the precaution of contacting
the responsible service organization.
We recommend concluding a service
contract with Leica Microsystems.
Repairs and Service Work
Only original Leica Microsystems spare
parts may be used.
Before opening the instruments, switch off
the power and unplug the power cable.
Avoid contact with powered electrical
circuits, which can lead to injury.
Service Address
In case of problems, please contact us as
Contact address
Leica Microsystems (Schweiz) AG
Industry Division
Max Schmidheiny Strasse 201
CH-9435 Heerbrugg (Switzerland)
Leitz Optilux User Manual
Important Notes12
Electrical Data and Ambient Conditions
Fuse Replacement
Unplug the instrument before changing any fuses. The Leitz Optilux has two fuses, which are located behind the power cord receptacle.
In order to maintain this condition and to ensure safe operation,
the user must follow the instructions and warnings contained in
this instruction manual.
Temperature for use
+10 °C … +40 °C
Storage temperature
-20 °C … +52 °C
Electrical Data
Input: 100–240 V, 50/60 Hz, 5 W (3 W LED)
+50 °F to +104 °F
Manipulation shock
25 mm on 50 mm hard wood
General safety notes
This instrument of safety class 1 has been built and tested in accordance
with the following safety requirements for electrical equipment for measurement, control, and laboratory use:
Transport shock (unpacked)
100 g / 6 ms
Transport shock (packed)
800 mm free fall
Transport vibrations (unpacked)
5–200 Hz / 1.5 g
Air pressure during use and storage:
500–1060 mBar
Humidity during use and storage:
20–90 %
Only use fuses of the following type: 5×20 mm, 1.0 A/250 V, high
breaking capacity (# 13RFAG30003)
EN 61010-1: 2002-08
EN 61010-2: 2008-06
IEC 61010-1: 2010-06
EN 61326-1: 2006-10
EN 61326-2-6: 2006-10
Leitz Optilux User Manual
EN 55011: 2007+A2: 2010-05
EN 60825-1: 2008-05
IEC 60825-1: 2007-03
LED Class 1
Installation Category II (Overvoltage Category)
Pollution degree 2
Important Notes13
Assembling the Leitz Optilux
Leitz Optilux User Manual
Assembling the Leitz Optilux14
Carefully remove the microscope and any
components from the packaging.
Check the components against the planned
Optional items such as contrast accessories,
camera adapters, cameras, and carrying
cases are not shipped as part of the standard equipment. These items are delivered
in separate packages.
Please do not discard any of the packing
materials. They should be used for safely
storing and transporting the instrument
should the need arise.
Leitz Optilux User Manual
Assembling the Leitz Optilux15
Identifying your Tube
All tubes are connected via a dovetail
and can be installed in any orientation.
Monocular "EZTube"
Leitz Optilux User Manual
Assembling the Leitz Optilux16
Installing the Monocular Tube
Tools used
OO Allen key
1. Loosen the setscrews (on top of the stand)
using the Allen key provided.
3. Turn the tube in the direction you desire.
4. Tighten the set screws on the left and right,
without applying too much force.
2. Insert the dovetail of the monocular tube
into the mount.
The eyepieces are already integrated in
the Leica "EZTube" and preset; for this
reason, the eyepieces do not require adjustment or assembly.
Leitz Optilux User Manual
Assembling the Leitz Optilux17
Removing and Inserting Objectives
The objectives are already installed
at delivery. The following description
shows how to (un)install the objectives if they
have to be removed for cleaning or replaced by
other objectives.
Your Leitz Optilux is delivered with objectives with magnification levels 4×, 10×
and 40×, possibly also with a 100× objective.
Otherwise, this can be retrofitted at any time.
Always turn the objective nosepiece by
its knurled ring. Never turn the objective
nosepiece by using an objective as a handle.
Removing Objectives
1. Unscrew the objectives to remove them.
The proper Sequence
When inserting the objectives, you can
begin with any available space on the
microscope. Continuation of the sequence,
however, has to be selected such that the
magnification always increases when turning
Continued on next page.
Leitz Optilux User Manual
Assembling the Leitz Optilux18
Removing and Inserting Objectives (continued)
Inserting Objectives
1. Screw the 4× objective into any available
space on the objective nosepiece.
2. Turn the objective nosepiece clockwise one
3. Screw in the next-largest objective.
4. Repeat steps 2 and 3 until all objectives
have been installed.
Leitz Optilux User Manual
Assembling the Leitz Optilux19
Installing the Mirror (optional)
The mirror is available as an optional
accessory. By means of this, the ambient
light can be used for illuminating the sample if
no power source is available for the built-in LED
Installing the Mirror
1. Position the microscope at a well-lit location, for example before a window.
Removing the Mirror
1. Simply pull the mirror out of the holder.
2. Press the mirror onto the mount of the illuminator.
The mirror is equipped with a flat and
a concave surface. Try them out to see
which side works better.
3. Look through the eyepieces and align the
mirror so that it works best.
Leitz Optilux User Manual
Assembling the Leitz Optilux20
Installing the Polarizer Kit (optional)
The polarizer kit is not included in the
delivery package, but available as an
optional accessory.
1. If you have already installed the tube,
remove it (see page 17).
2. Place the analyzer with the flat side facing
down into the beam path. The two screws
have to rest in the two existing holes.
4. Place the polarizer onto the aperture of the
illuminator. The handle must come to rest
in the aperture with the white arrow pointing upwards.
3. Install the tube.
5. Turn the polarizer to align it. The arrows
indicate the current orientation.
Leitz Optilux User Manual
Assembling the Leitz Optilux21
Using the Leitz Optilux
Leitz Optilux User Manual
Using the Leitz Optilux22
Switching on the Microscope
safety reasons, the power cable
is grounded via the microscope base.
Never use an adapter between the power cable
and the power supply; that would render the
grounding ineffective.
Startup Procedure
1. Attach the power cable to the rear side of
the microscope.
3. Switch on the microscope using the toggle
switch on the rear side of the stand.
2. Set the illumination to the lowest setting
using the controller on the bottom left of
the stand.
Work Surface
Always use the microscope on a hard,
stable surface.
Leitz Optilux User Manual
Using the Leitz Optilux23
Selecting an Objective
Using the Condenser
The condenser is furnished with an iris
diaphragm, which has to be adjusted to
match the effective numerical aperture of the
individual objectives.
Leitz Optilux User Manual
Selecting an Objective
1. Rotate the desired objective over the
2. Rotate the condenser diaphragm below the
specimen stage so that the displayed value
matches the selected objective.
Using the Leitz Optilux24
Preparing for Viewing
1. Open the specimen holder using the
tension lever.
Leitz Optilux User Manual
2. Insert the specimen slide and slowly release
the tension lever.
3. Using the X/Y control, position the specimen slide such that a part of the specimen
is under the objective used.
Using the Leitz Optilux25
1. Use the knurled ring to rotate the objective
nosepiece in such a way that the objective with the lowest magnification level is
turned into the working position.
Leitz Optilux User Manual
2. Move the specimen stage upwards as far as
it will go using the coarse adjustment knob.
3. Look into the eyepieces and adjust the illumination to a comfortable intensity.
4. Bring the specimen into focus using the
fine adjustment knob.
Using the Leitz Optilux26
Adjusting the Eyecups
If you wear eyeglasses for microscope
viewing, keep the rubber eyecups folded
down. If you do not wear eyeglasses, you may
find it useful to unfold the rubber eyecups in
order to help block out ambient room light.
Leitz Optilux User Manual
Potential Sources of Infection
Direct contact with eyepieces is a potential transmission method for bacterial
and viral infections of the eye. The risk can be
minimized by the use of individual eyecups.
Eyecups can be ordered separately. Please
contact your Leica partner.
Using the Leitz Optilux27
Oil Immersion Technique
Oil Immersion Technique
1. Search for the area on the specimen slide
that you want to study.
3. Add a drop of immersion oil from Leica
to the area of the specimen slide you are
2. Move the specimen stage down to the
lowest position using the coarse adjustment knob.
5. Slowly move the specimen stage upwards
using the coarse adjustment knob until
the oil drop on the slide comes into direct
contact with the lens of the oil immersion
4. Rotate the 100× objective to the working
6. Hold the knurled ring on the objective
nosepiece and swing the objective back
and forth to eliminate air bubbles. Then
bring the oil objective into the final position, so that the oil drop is between the
front lens of the objective and the specimen slide.
Leitz Optilux User Manual
Using the Leitz Optilux28
Oil Immersion Technique (continued)
7. Look through the microscope and slowly
rotate the fine adjustment knob, so that the
specimen stage moves upwards until the
specimen is in focus.
8. If you are finished w orking w ith t he o il
immersion objective, clean the front side
of the objective, the specimen slide, and all
other surfaces that have come into contact
with the oil, following the instructions in
the "Care of the Microscope" section on
page 30.
Leitz Optilux User Manual
Using the Leitz Optilux
Care of the Microscope
Leitz Optilux User Manual
Care of the Microscope30
Maintenance instructions
General Notes
OO Protect the microscope from moisture,
fumes and acids and from alkaline, caustic
and corrosive materials. Do not store chemicals in the vicinity.
Protect the microscope from oil and grease.
Never grease or oil mechanical parts or sliding surfaces.
Follow the instructions of the disinfectant
We recommend concluding a service
contract with Leica Service.
Leitz Optilux User Manual
Cleaning coated Parts and plastic Parts
Dust and dirt particles should be removed
with a soft brush or lint-free cotton cloth.
Remove coarse debris with a moistened
disposable cloth.
Acetone, xylene or nitro-containing thinners must NOT be used.
Never use chemicals to clean colored
surfaces or accessories with rubberized
parts. This could damage the surfaces,
and specimens could be contaminated by
abraded particles.
Care of the Microscope31
General Maintenance
Always use both hands to carry the
Leitz Optilux User Manual
Keep all optical components clean. Cleanliness is important for maintaining good
optical performance.
Optical surfaces should be cleaned using a
lint-free cloth with a commercially available
glass cleaner.
When the microscope is not in use, it
should always be put away with protection
against dust.
If any optical surface becomes coated with
dust or dirt, clean the surface by blowing it
off with a syringe or brushing it off with a
camel hair brush before attempting to wipe
the surface clean.
Use solvent only very sparingly, so that it
does not flow out over the lens. No part
of the microscope is quite so vulnerable to
collecting dirt, dust, and oil as the front lens
of the objective. Whenever you encounter
lack of contrast, cloudiness or poor definition, carefully check the condition of the
front lens with a magnifier.
Care of the Microscope32
General Maintenance (continued)
The lower magnification objectives have
fairly large front lenses and can be cleaned
with a cloth or lens tissue wrapped around a
finger and moistened with window cleaner.
The Leitz Optilux uses an LED illuminator.
Therefore, no lamp changing is required for
the entire life of the microscope.
Cleaning 40× and 100× objectives requires
more care. To attain the typical flatness for
objectives with higher magnification, the
objective has a small concave front lens
with a very small radius and slight curvature. The surface of this front lens can be
readily cleaned with a toothpick covered
with a cotton tip, or with a small cotton
swab. Wipe the front lens lightly without
applying undue force or scrubbing action.
Make sure that the cotton tip contacts the
concave lens surface. Check the objective
with a magnifier after cleaning.
Leitz Optilux User Manual
Care of the Microscope33
Leitz Optilux User Manual
side view with monocular tube
Leitz Optilux User Manual
front view with monocular tube
side view with monocular tube 180°
rotated for storage
The productive cooperative effort "with the user, for the user" has always been the
basis for the innovative strength of Leica Microsystems. On this, we have developed
our five corporate values:
pioneering, high-end quality, team spirit, dedication to science, and continuous
improvement. We call making these values reality Living up to Life.
The Leica Microsystems Industry Division's focus is to support customers' pursuit of
the highest quality end result by providing the best and most innovative imaging
systems for their needs to see, measure, and analyze microstructures. Its solutions
are used in routine and research industrial applications, in materials science and
quality control, in forensic science investigations, and educational applications.
Leica Microsystems – an international company with an experienced worldwide
customer service network.
Internationally active
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13IDE14060EN ∙ Copyright © by Leica Microsystems (Schweiz) AG, CH-9435 Heerbrugg, 2014.
Subject to modifications. LEICA and the Leica Logo are registered trademarks of
Leica Microsystems IR GmbH.
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