1 148 Series User`s Manual
148 Series
User’s Manual
1
148 Series
Table of Contents
Before Use............................................................................................. 3
Introduction.............................................................................................................3
Safety Precautions...................................................................................................4
Parts....................................................................................................... 5
M148........................................................................................................................5
Definition of Parts...................................................................................................6
Getting Started..................................................................................... 7
Assembly..................................................................................................................7
Operation.............................................................................................. 8
Setting Up................................................................................................................8
Focusing...................................................................................................................8
Adjusting The Diaphragm.......................................................................................8
Attaching a Camera / Changing Eyepieces.............................................................8
Setting the Stage’s Stop-Limit..................................................................................9
Adjusting Focusing Tension....................................................................................9
Maintenance/Precautions.....................................................................................10
Specifications...................................................................................... 11
148 Series Specifications........................................................................................11
Optional Accessories..............................................................................................12
Objectives...............................................................................................................13
Eyepieces................................................................................................................13
Technical Parameters........................................................................ 14
Electrical System....................................................................................................14
Technical Terms & Concepts.................................................................................15
Common Issues......................................................................................................16
Common Issues (Continued)................................................................................17
2
148 Series
Before Use
Introduction
Congratulations on the purchase of your new AmScope microscope!
This manual is designed for the M148 series microscopes.
Please be sure to take a few minutes to familiarize yourself with the features and functions of your
new AmScope microscope.
If you’d like more information on microscopes, parts, or accessories, please visit our website at:
www.iScopeCorp.com
We highly recommend you study this manual thoroughly before operating the microscope, and
that yo ukeep it on ahnd for future reference.
If you have additional questions or need assistance, please send us an email at:
[email protected]
3
148 Series
Before Use
Safety Precautions
1. As the microscope is a precision instrument, always handle it with care, avoiding impact
or abrupt movement during transporation. Do not shake the package.
2. Do not place the microscope in direct sunlight or in high heat. Keap it indoors in a dry
and clean place with temperateures between 32-100 degrees F (0-40 degrees C), and in
maximum relative humidity of 85%.
3. Avoid touching the lenses on the objectives and the eyepieces so that oil and dirt from
your fingerprints do not obstruct your view.
4. Before turning the power on, make sure that the power supply voltage is consident with
the voltage of your microscope.
4
148 Series
Parts
M148
Eyepiece
Ocular Tube
Rotating Monocular Head
Nosepiece Turret
Objectives
Limit Stop
Stage Clips
Stage
Disc Diaphragm (Disc Iris)
Focusing Knob
Illuminator Lens
5
148 Series
Parts
Definition of Parts
Base Illuminator Lens
Directs the light source towards the slide
Focusing Knob
Used to bring the slide into the sight and focus
Disc Iris Diaphragm
Controls the amount of light that hits the slide from the base illuminator
Limit Stop Knob
Limits the upward movement of the mechanical stage in order to avoid damaging the slide and objective
Nosepiece
Houses the objective lenses
Rotating Monocular Head
Houses the eyepiece and optics of the microscope
6
148 Series
Getting Started
Assembly
1. First, take the styrofoam container out of the cardboard carton and lay it on its side, paying attention to which
side is labeled up. Remove the tape and open the container carefully so as to avoid dropping and damaging the
optical items. Check carefully to ensure that all parts and accessories are intact.
2. Check the packing list to ensure that you’re received all items:
- One Monocular Microscope Body & Head
- Three Objectives (4x, 10x, 40x)
- 10x Widefield Eyepieces
- 16x Widefield Eyepieces (for -A models only)
- 20x Widefield Eyepieces (for -B models only)
- 25x Widefield Eyepieces (for -C models only)
- One Dust Cover
Note: LED models do not have a spare bulb, as the LED bulbs do not need replacing.
3. Remove the microscope body from the box and remove the plastic protective covering. The body of the microscope is composed of the base, the stage, the arm, and the nosepiece.
4. Screw the objectives into the microscope nosepiece from the lowest magnification to the highest, again avoiding touching the lenses.
5. Plug in the microscope and turn it on. If no light emerges from the light source, adjust the dimmer knob on
the side of the base.
7
148 Series
Operation
Setting Up
1. Place the specimen to be studied on a glass slide (or use a prepared slide). Place it on the stage, holding it snugly
in place with the metal slide holders (clips) of the mechanical stage.
2. Center the specimen over the stage opening, lining it up with the light and the objective lens.
Focusing
1. Turn the nosepiece to choose an objective. It is easiest to use the lowest magnification first (4x objective) to locate and focus on the specimen. As you move up in magnification you may need to refocus the image a little each
time.
2. Being focusing by first looking with one eye through the eyepiece without the diopter. Close your other eye. Use
the coarse focusing knob to adjust the height of the stage until the sample comes into clear focus.
Note: You may loosen the limit-stop knob (located on the read of the stage) in order to give yourself the full range
of motion for fine tuning the focus.
3. Once the image is clear in your field of view, you will want to use the fine focusing knob to tune it for best results.
Note: Please be careful when moving the mechanical stage if you need to recenter the sample, or if moving the
stage very close to the objectives. The limit stop is designed to prevent impact between objective and slide, so when
it is off you will be able to damage the microscope.
Adjusting The Diaphragm
1. By changing the aperture (hole size) of the iris diaphragm, you can adjust the background brightness. Adjust the
aperture of the iris diaphragm by rotating the disc underneath the stage to the desired aperture.
Attaching a Camera / Changing Eyepieces
1. To remove the 10x eyepiece included on the microscope, you will need to remove the small metal screw on the
ocular tube just under where the eyepiece connects to the tube. To do so, you will need a 1mm flathead precision
screwdriver (much like what you would use to adjust eye glasses).
2. Once unscrewed, remove the eyepiece and slide in the camera or alternate eyepieces.
8
148 Series
Operation
Setting the Stage’s Stop-Limit
1. To adjust the stop limit on the stage, unlock it by unscrewing the lower nut. The stop limit is located at the rear
of the stage.
2. Once tension is released on the nut by rotating it counter clockwise, you may adjust the upper thumb screw to
move the limit up or down as desired.
3. Lock the limit stop nut back into place after the desired stage height is achieved.
9
148 Series
Operation
Maintenance/Precautions
- All glass surfaces must be kept clean. Fine dust on the optical surface should be blown off using a can of compressed air or gently wiped off with a soft lens paper tissue/nonabraisive lint free cloth.
- Carefully wipe off oil or fingerprints on the lens surfaces using tissue moistened with a small amount of lens
cleaner (we recommend Sparkle brand optical cleaner).
- Do not use Sparkle to clean other elements of the microscope. Use a neutral detergent on any plastic or painted
surfaces.
- Do not assemble or disassemble the microscope’s electrical components yourself without advisement from one of
our technicians. Doing so will void your warranty unless by advisement of one of our technicians to do so.
- After use, cover the microscope with the provided dust cover.
- Keep your AmScope microscope in a dry, clean location in order to prevent rust or other damage.
- To change batteries with this unit, use the included allen wrench (hexagonal shaped L-tool) to undo the door hex
screw on the base. Use 3 AAA batteries with the unit.
- The unit can be used as a recharger for AAA batteries specified as rechargeable only. Please do not use standard
batteries in the unit with the charger plugged in to avoid damage.
10
148 Series
Specifications
148 Series Specifications
Parts
Specifications
WF Eyepiece
WF10X/18mm
M148
WF10X/18mm w/ Pointer
M148A
x
M148B
M148C
x
x
x
WF10X/18mm w/ Reticle
WF16X/18mm
x
WF20X/18mm
x
WF25X18mm
Plan Eyepiece
x
P5X
P10X
P16X
DIN Achromatic Objectives
4X/0.10
x
x
x
x
10X/0.25
x
x
x
x
40X(spring)/0.65
x
x
x
x
Monocular, 360 Degree Swiveling
x
x
x
x
Diaphragm
Iris Diaphragm Disc
x
x
x
x
Illumination
Halogen Light w/ Dimmer
x
x
x
x
60X(spring)/0.85
100X(spring, oil)/1.25
Plan Objectives
4X
10X
40X(spring)
100X(spring, oil)
45 Degree Viewing Head
Binocular Sliding, 360 Degree Swiveling
Trinocular Sliding, 360 Degree Swiveling
LED Light
Lamp
6V/20W
6V/30W
LED
Filter
x
Blue/Yellow/Green
11
x
x
x
148 Series
Specifications
Optional Accessories
Parts
Description
Eyepiece
5X
EP5X18
Obtaining 20x, 50x, 200x, and 500x magnification
powers
20x
EP20X18
Obtaining 80x, 200x, 800x, and 2000x magnification powers
25x
EP25X18
For obtaining 250x and 2500x magnification powers
10x w/ Pointer
EP10X18P
For easier identifying of objects
10x w/ Reticle
EP10X18R
For measuring objects
2X
A2X
For obtaining 20x and 32x magnification powers
5X
A5X
For obtaining 50X and 80X magnication powers
20X
A20x
For obtaining 200x and 320x magnification powers
60X
A60X
For obtaining 600x and 960x magnification powers
Plan 4X
PA4X
For obtaining higher clarify in images
Plan 10X
PA10X
For obtaining higher clarify in images
Plan 40X
PA40X
For obtaining higher clarify in images
Plan 100X
PA100X
For obtaining higher clarify in images
MU035 (350k)
MU130 (1.3mp)
MU300 (3mp)
MU500 (5mp)
MU800 (8mp)
MU900 (9mp)
MU1000 (10mp)
To capture images, video, or view live display on a
computer (PC/Mac OS X)
MR400
To calibrate the camera software for on screen
measurements
CCD TV/Video
CCD-NP
To view live display on a television (RCA)
Aluminum Case
AC-B100
For carrying microscope around safely
Objective
Camera
CMOS Digital
Calibration Micrometer
Case
Model #
12
Purpose
148 Series
Specifications
Objectives
Type
DIN Achromatic
Objective (195mm)
Plan Objective
(195mm)
Magnification
Numerical Aperture
(N.A.)
Medium
Parfocal Distance (mm)
4X
A2X
Air
45
Red
10X
A5X
Air
45
Yellow
40X
A20x
Air
45
Light Blue
60X
A60X
Air
45
Deep Blue
100X
A100X
Cedar Oil 45
Magnification Marks
(Color Ring)
White
Plan 4X
PA4X
Air
45
Red
Plan 10X
PA10X
Air
45
Yellow
Plan 40X
PA40X
Air
45
Light Blue
Plan 100X
PA100X
Cedar Oil 45
White
Eyepieces
Type
Widefield Eyepiece
Medium
Plan Eyepiece
Magnification
10X
15X
20X
5X
10X
16X
Field of View
Φ18
Φ13
Φ11
Φ18
Φ18
Φ15
13
148 Series
Technical Parameters
Electrical System
There are two options for eletrical systems for this series of microscope. The light source is an LED system.
1. 220V~240V power supply: 220V~240V ±10%, 50Hz
This electrical system is CE and GS certified
2. 100V~120V power supply: 100V~120V ±10%, 60Hz
This electrical system is UL certified.
All units come standard as 110V units unless an upgrade to a 220V system is requested. Upgrade fee is dependent
on which unit is purchased.
This unit is also capable of using batteries and charging rechargable batteries. It uses 3 AAA batteries. When
plugged in with batteries in the unit, the batteries will be charged.
Note: Please do not use the recharging feature with standard batteries, as damage to your unit can occur.
14
148 Series
Technical Parameters
Technical Terms & Concepts
Total Magnification
Total magnification of a microscope is calculated by the magnification of the objective multiplied by the
magnification of the eyepieces.
-Ex: (10x Eyepieces) x (4x Objective) = 40x Total Magnification
Field of View
Linear field of view of the eyepiece divided by the magnification of the objective
Numerical Aperture (N.A)
Calculated by n Sin α (max), the Numerical Aperture (N.A) is an important parameter that marks the fetaures of the objective and condenser’s image quality and resolution. The “n” is a refractive index of the medium
(air or immersion cedar oil) between the omjective lens and the specimen. The “α” is 1/2 of the angle between the
aperture on the objective and path of light. The larger the N.A, the higher the resolution of the objective (and better
quality of the image).
Object to Primary Image Distance
The distance between the object plane and the primary image plane. The conjugate distance is fixed.
Mechanical Tube Length
The distane between the objective shoulder and the ocular shoulder
15
148 Series
Troubleshooting
Common Issues
Symptom
Cause
Remedy
OPTICAL ISSUES
One side of the field
of view is darker
The nosepiece if misaligned
Turn the nosepiece until it clicks into place
Stains or dust has accumulated on the objective,
eyepieces, or base lens
Clean all lenses with lens cleaner or a lint free nonabraisive cloth
Obstructions are
observed in the field
of view
Stains, dust, or dirt has accumulated on the specimen
Clean the slide or use a new specimen if sample is
destroyed
Stains, dust, or dirt have accumulated on the lens
Clean the lens
Unclear Image
There is no cover slip on the slide
Add a cover slip. The objectives are designed for use
with a 0.17mm cover slip, so it is a requirement to
use one for proper images.
The cover slip is not standard sized
Replace the cover slip with the appropriate 0.17mm
thickness slip
The aperture is not open to an appropriate diameter
Adjust the aperture to have the light just larger than
the size of the condenser
Stain or dust has accumulated on the lens in the
inlet of the head
Clean the lens with lens cleaner or a nonabraisive
lint free cloth, as well as spray with compressed air
The specimen slide is not fixed
Secure the slide to the stage with clips
The nosepiece is not in the right position
Turn the nosepiece until it clicks into place
One side of the field
of view is dark or the
image moves while
focusing
The field of view is not The iris disc diaphragm is not big enough
bright enough
Stains, dust, or dirt has accumulated on the condenser, objective, eyepieces, or base lens
16
Rotate the iris disc diaphragm to allow more light
to travel
Thoroughly clean tall lenses with lens cleaner or a
lint free nonabraisive cloth
148 Series
Troubleshooting
Common Issues (Continued)
Symptom
Cause
Remedy
MECHANICAL ISSUES
The objective touches the
cover slip
The cover slip is not standard sized
Replace the cover slip with the appropriate 0.17mm thickness slip
The limit-stop is set too high or not engaged
Be careful to avoid contact between objective and the slide when
the limit stop is not engaged. To reengage, focus the sample, then
lock the limit stop into place to set max height at a safe but usable distance.
Unable to move the slide
smoothly
The slide is not secured correctly
Adjust the slide to use the stage clips and secure the sample
The mechanical stage is not properly secured
Tighten the mechanical stage screws to better secure the stage
Focus knob does not turn
The tension knob is too tight
Loosen it by adjusting the tension ring inside the coarse focus
knob counterclockwise (close to the arm of the microscope on
the left of the microscope)
Stage declines by itself
The tension knob is too lose
Tighten it by adjusting the tension ring inside the coarse focus
knob clockwise (close to the arm of the microscope on the left
of the microscope)
The focusing knob won’t
raise the stage
Limit-stop is engaged
Disengage the limit stop on the rear of the stage of the microscope
ELECTRICAL ISSUES
The bulb/light source
flickers
The bulb is close to burning out
Please contact us in regards to this issue. LED lights do not burn
out, so there may be another electrical issue
The microscope does not
light up
The microscope is unplugged
Insert the plug into the wall socket to achieve electrical illumination
17
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