TProfessional user manual

TProfessional user manual
Instruction Manual
Ver. 09/07
•
Model
Order No.
TProfessional 60
TProfessional 96
TProfessional Gradient 60
TProfessional Gradient 96
TProfessional 384
070-900
070-901
070-800
070-801
070-902
Please read these instructions carefully before using this
apparatus!
•
Biometra GmbH
Rudolf-Wissell-Str. 30
D-37079 Göttingen
Tel.: ++49 (0)551-50 68 6-0
Fax: ++49 (0)551-50 68 6-66
email: [email protected]
Internet: http://www.biometra.com
Service Department
Rudolf-Wissell-Strasse 14-16
D-37079 Göttingen
Tel.: ++49 (0)551-50 68 6-10 or -12
Fax: ++49 (0)551-50 68 6-11
email: [email protected]
This document describes the state at the time of publishing. It needs not necessarily agree with
future versions.
Subject to change!
2
Instruction Manual TProfessional 07/2009
Contents
1
Introduction......................................................................................................................... 6
1.1
Field of Applications ..................................................................................................... 6
1.2
Special features ............................................................................................................ 6
1.2.1
TProfessional Thermoblock .................................................................................. 6
1.2.2
TProfessional Lid .................................................................................................. 6
1.2.3
TProfessional User interface ................................................................................ 6
1.2.4
TProfessional Housing.......................................................................................... 6
1.3
Technical specifications................................................................................................ 8
1.4
Legal Notes .................................................................................................................. 9
1.4.1
PCR License – Legal Disclaimer .......................................................................... 9
1.4.2
Copyright .............................................................................................................. 9
1.4.3
Liability.................................................................................................................. 9
1.4.4
Meaning of the Instructions................................................................................... 9
2
Safety and Warning Notices ............................................................................................ 10
2.1
2.2
3
Installation......................................................................................................................... 13
3.1
3.2
3.3
3.4
3.5
4
Content of delivery...................................................................................................... 13
Unpack and Check ..................................................................................................... 13
Installation Conditions ................................................................................................ 13
Operation Voltage....................................................................................................... 14
Initial self test (power on self test) .............................................................................. 15
Operating elements .......................................................................................................... 16
4.1
4.2
4.3
4.4
4.5
5
Definition of Symbols .................................................................................................. 10
General Safety Instructions ........................................................................................ 10
TProfessional Thermocycler front view....................................................................... 16
TProfessional Thermocycler rear view ....................................................................... 16
Exchange of block module ......................................................................................... 17
The TProfessional control panel ................................................................................. 17
High Performance Smart Lid (HPSL).......................................................................... 18
Operating........................................................................................................................... 19
5.1
The TProfessional User Interface ............................................................................... 19
5.1.1
TProfessional Menu Buttons............................................................................... 19
5.1.2
TProfessional Soft Keys ..................................................................................... 19
5.1.3
Log in Menu ........................................................................................................ 20
5.1.4
Block Menu ......................................................................................................... 20
5.1.5
System Menu...................................................................................................... 21
5.1.6
Program Menu .................................................................................................... 21
5.2
TProfessional software ............................................................................................... 22
5.2.1
Navigation within software menus ...................................................................... 22
5.2.2
Log in .................................................................................................................. 22
5.2.3
Log in existing User ............................................................................................ 22
5.2.4
Create new user account.................................................................................... 24
5.2.5
Supervisor Log in................................................................................................ 25
Instruction Manual TProfessional 07/2009
3
5.2.6
Delete User .........................................................................................................25
5.3
Create program ...........................................................................................................25
5.3.1
Create program from template ............................................................................26
5.3.2
Create program without template ........................................................................26
5.3.3
Set block type......................................................................................................27
5.3.4
Set lid pre-heating mode .....................................................................................28
5.3.5
Set lid temperature ..............................................................................................29
5.3.6
Enter step temperature and time .........................................................................29
5.3.7
Set loop ...............................................................................................................30
5.3.8
Cool below ambient temperature ........................................................................31
5.3.9
Program Pause ...................................................................................................31
5.3.10
Save program ......................................................................................................31
5.3.11
Set Gradient ........................................................................................................34
5.3.12
Program linear gradient .......................................................................................35
5.4
Edit programs ..............................................................................................................36
5.4.1
Insert program step .............................................................................................36
5.4.2
Delete program step ............................................................................................38
5.4.3
Copy program......................................................................................................39
5.4.4
Delete program....................................................................................................40
5.4.5
Editing programs in the graphical mode ..............................................................41
5.5
Change program options.............................................................................................42
5.5.1
Heating and cooling rate .....................................................................................43
5.5.2
Time increment Æ Δt(s).......................................................................................43
5.5.3
Temperature increment or decrement Æ ΔT(°C) ................................................44
5.6
Run program ...............................................................................................................44
5.6.1
Select program for start from User directories.....................................................44
5.6.2
Quick start programs from the block Menu..........................................................45
5.6.3
View program prior to start ..................................................................................45
5.6.4
Display during operation......................................................................................46
5.6.5
Pause Program during run ..................................................................................47
5.6.6
Continue program................................................................................................48
5.7
Stop program...............................................................................................................48
5.7.1
Stop Program from a pause ................................................................................48
5.8
Optimization of Fast-PCR programs ...........................................................................48
6
System settings.................................................................................................................51
6.1
User configuration .......................................................................................................51
6.1.1
Set language .......................................................................................................51
6.1.2
Change individual PIN .........................................................................................51
6.1.3
Delete Account ....................................................................................................52
6.2
System configuration...................................................................................................52
6.2.1
Configure beep....................................................................................................52
6.2.2
Configure Contrast ..............................................................................................53
6.2.3
Set time and date ................................................................................................54
6.2.4
Defragment memory............................................................................................54
6.2.5
Screen saver .......................................................................................................54
6.3
System info..................................................................................................................55
6.3.1
System info..........................................................................................................55
6.3.2
View Log files of the last five runs .......................................................................56
6.4
Service ........................................................................................................................56
4
Instruction Manual TProfessional 07/2009
6.4.1
View log files of Power on self test ..................................................................... 57
6.4.2
Execute extended self test.................................................................................. 57
6.4.3
View log files from extended self test ................................................................. 58
6.4.4
View Error log files.............................................................................................. 58
6.4.5
Create Info file for Biometra Service Department ............................................... 58
6.5
How to contact Biometra ............................................................................................ 59
6.6
Supervisor system configuration................................................................................. 59
6.6.1
Change user PIN ................................................................................................ 59
6.6.2
Delete user account............................................................................................ 59
6.6.3
Change supervisor PIN....................................................................................... 59
6.6.4
Set boot language............................................................................................... 60
6.6.5
Delete all user accounts ..................................................................................... 60
7
Short manual..................................................................................................................... 61
8
Trouble shooting .............................................................................................................. 66
8.1
8.2
8.3
8.4
8.5
8.6
9
Slow heating and cooling............................................................................................ 66
Autorestart .................................................................................................................. 66
Autorestart due to unrecognised power failure ........................................................... 66
Adaptation of protocols from other cyclers ................................................................. 66
Releasing wheel in case of blocked lid ....................................................................... 66
Recommendations for the use of 384 well Thermocyclers ......................................... 67
Maintenance and repair ................................................................................................... 68
9.1
9.2
9.3
9.4
10
Accessories ...................................................................................................................... 69
10.1
10.2
11
Cleaning and Maintenance ......................................................................................... 68
Servicing and repair.................................................................................................... 68
Firmware update......................................................................................................... 68
Replacement of Spare Parts ...................................................................................... 68
Block modules ............................................................................................................ 69
Plasticware ................................................................................................................. 69
Service............................................................................................................................... 70
11.1
11.2
Instructions for return shipment .................................................................................. 70
Packing of the Thermocycler ...................................................................................... 71
12
Equipment Decontamination Certificate ........................................................................ 72
13
Note for the disposal of electric / electronic waste....................................................... 74
14
Declaration of Conformity ............................................................................................... 75
15
Warranty ............................................................................................................................ 76
16
Subject Index .................................................................................................................... 77
Instruction Manual TProfessional 07/2009
5
1
Introduction
1.1 Field of Applications
The Biometra TProfessional is a Thermocycler that is licensed for PCR applications (research
use only) and intended to be used for performing polymerase chain reactions. The development
of the instruments was inspired by the wish to simplify molecular biology research. To reach this
goal the instrument housing, lid and block were totally redesigned, providing so far unrealised
speed and temperature uniformity. Innovative user software was developed, driven by
Biometras 15 years experience in interface design. The result is an easy to use Thermocycler
with excellent technical specifications.
1.2 Special features
1.2.1
TProfessional Thermoblock
The TProfessional Thermocycler offers five different block modules. To achieve ultimate
performance the 60 well and 96 well modules are made of silver. Due to its excellent heat
conductivity silver equilibrates extremely quickly, thus providing maximum speed and
temperature uniformity. To protect the valuable silver blocks against corrosion, the block
surfaces are gold plated.
The 60 well silver block module can be used with 0.5 ml tubes. The 96 well silver block module
is designed for daily routine work and can be used with 0.2 ml tubes, strips or microplates. For
rapid optimisation of new protocols, a 60 well and a 96 well Gradient version are available. The
384 well block module is designed for use with 384 well plates. All block modules can be easily
exchanged in less than 10 seconds.
1.2.2
TProfessional Lid
The TProfessional lid has been optimised to achieve two essential requirements: prevention of
condensation and reliable contact between samples and thermoblock. The lid is powered by
Biometras well know smart lid technology which automatically limits lid pressure to save tubes
from damage. In addition, the new design of the High Performance Lid ensures an even
temperature distribution between samples thus significantly improving temperature uniformity.
With one press on the front button, the lid gently swings open and arrests in its end position.
1.2.3
TProfessional User interface
The TProfessional user interface incorporates Biometras proven spreadsheet-programming
philosophy. Avoiding endless serial program windows, all steps of a program are entered in a
single screen. This makes the creation of new or editing existing programs fast and easy. One
touch leads from the spreadsheet to an alternative graphical programming mode. The
TProfessional manages up to 30 individual users (all password protected) and provides for a
large program store.
1.2.4
TProfessional Housing
The elegant TProfessional housing is designed for heavy use. Due to an optimised air stream
design even higher efficiency and better temperature uniformity is achieved. The instrument
works quietly and consumes little power, which in turn leads to low heat emission. The compact
footprint saves valuable bench space. Keyboard and display have been set to an angle that
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Instruction Manual TProfessional 07/2009
ensures reflection-free viewing and ergonomic programming. To facilitate servicing the
TProfessional housing can be completely removed from the chassis.
Instruction Manual TProfessional 07/2009
7
1.3 Technical specifications
070-900
070-800
070-901
070-801
TProfessional 60
TProfessional Gradient 60
TProfessional 96
TProfessional Gradient 96
Block
60 tubes 0.5 ml/
96 tubes 0.2 ml,
96 well microplates
Silver
60 tubes 0.5 ml/
96 tubes 0.2 ml,
96 well microplates
Silver
Block coating
Gold
Gold
Special Alloy
Block exchange
Quick-X-change
Quick-X-change
Quick-X-change
Block exchange time
less than 10 sec
less than 10 sec
less than 10 sec
Max. Heating Rate*
6.0 °C/sec
6.0 °C/sec
2.2 °C/sec
Max. Cooling Rate*
4.5 °C/sec
4.5 °C/sec
1.5 °C/sec
Avg. Heating Rate*
5.0 °C/sec
5.0 °C/sec
1.7 °C/sec
Avg. Cooling Rate*
3.5 °C/sec
3.5 °C/sec
1.0 °C/sec
Gradient
-
40 °C
-
Order Number
Name
Capacity
Temperature Uniformity
070-902
TProfessional 384
384 well microplates
Aluminium
95 °C: +/- 0,50 °C within 15 sec
+/- 0.6°C
70 °C: +/- 0,25 °C within 15 sec
+/- 0.4°C
55 °C: +/- 0,15 °C within 15 sec
+/- 0.3°C
Temperature Range
3 °C to 99 °C
Control Accuracy
+/- 0.1 °C
Programming mode
Quick start of the last five programs, view program prior to start, toggle between
spreadsheet and graphical mode, extended self test, generate service files,
adjustable ramp rates, view gradient temperature graph, PC control via RS 232
Spreadsheet or graphical
Program memory
total capacity of 350 programs in 30 user directories (password protected)
Display
¼ VGA screen, 320 x 240 pixel
Auto restart after power
failure
Cool samples to 4°C
Yes
Smart Lid Technology
Yes
Lid Temperature Range
30 to 99 °C
Power consumption
480 Watt
Power Supply
100, 115, 230 Volt, 50-60 Hz
Noise emission
Very low
Interfaces
RS232 serial port
Working conditions
15 °C to 35 °C, 70 % air humidity, max 2000 m NN
Software
8
Yes
Instruction Manual TProfessional 07/2009
* Measured inside the block
1.4 Legal Notes
1.4.1
PCR License – Legal Disclaimer
Purchase of a Biometra Thermocycler conveys a limited non-transferable immunity from suit for
the purchaser’s own internal research and development and applied fields other than human in
vitro diagnostics under one or more of US Patents Nos. 5,038,852, 5,656,493, 5,333,675,
5,475,610, and 6,703,236, or corresponding claims in their non-US counterparts, owned by
Applera Corporation.
No right is conveyed expressly, by implication or by estoppel under any patent claim, reagents,
kits, or methods such as 5´ nuclease methods, or under any other apparatus or system claim,
including but not limited to US Patent No. 6,814,934 and its non-US counterparts, which
describe and claim thermal cyclers capable of real-time detection.
Further information on purchasing licenses may be obtained by contacting the Director of
Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
• Biometra biomedizinische Analytik GmbH
Rudolf Wissell Str. 30
37079 Goettingen, Germany
1.4.2
•
Copyright
All rights reserved. It is not allowed to copy and publish the manual or parts of it in any form as
copies, micro film or other methods without a written authorisation from Biometra.
Biometra is pointing out that applied company and brand names are usually protected trade
marks.
1.4.3
Liability
Biometra is not liable for damages and injuries caused by use not considering these operating
instructions in parts or completely.
1.4.4
Meaning of the Instructions
Biometra recommends that you first read these instructions carefully. This operation instruction
is part of the product and should be kept over the full life-time of the instrument. It should also
be forwarded to subsequent owners and users. Make sure that additions and updates are
inserted into the operation instructions.
Instruction Manual TProfessional 07/2009
9
2
Safety and Warning Notices
2.1 Definition of Symbols
Symbol
•
Definition
Caution! Refer to instruction manual!
Danger! High voltage!
Fragile!
Danger! Hot surface!
2.2 General Safety Instructions
Please read this manual carefully before starting operation of the TProfessional Thermocycler.
The TProfessional Thermocycler is intended for sample incubation at varying temperatures.
•
General safety precautions for laboratory work must be observed when working with the
TProfessional Thermocycler.
•
The TProfessional does not produce a sound power level that could be hazardous for the
user.
The thermoblock and the heated lid will reach high temperatures
during operation. Both thermoblock and heated lid can burn you.
Rapid heating of the thermoblock can cause liquids to boil
explosively. Always wear safety goggles during operation. Close the
lid before starting a program.
Do not heat samples without having the lid locked securely.
Be aware that samples are reaching high temperatures. Do not touch
or open hot tubes or microplates, because hot liquid may quickly spill
out.
Do not touch the heated lid.
Use only suited plastic ware in the TProfessional thermocycler.
Tubes and plates must show good fit when placed in the
thermoblock. Only use tubes that are suited for high temperatures
(tight lids).
10
Instruction Manual TProfessional 07/2009
The TProfessional Thermocycler contains no user serviceable parts.
Do not open the housing instrument. Service and repair may only be
carried out by the Biometra Service department or otherwise
qualified technical personal.
Do not use the instrument when damages of the housing, block,
cable or other parts are visible.
Prior to connecting the unit to the power source please ensure that
the voltage selector at the bottom of the instrument is set to the
required voltage.
Make sure that the main supply voltage is in accordance with the
label above the power connection (see section 4.2)
Unplug the power cable before you open the TProfessional
Thermocycler. Danger of electric shock!
Make sure that the appliance connector and the plug of the supply
cord are accessible, so you can separate the instrument from the
mains.
Connect the TProfessional Thermocycler to a grounded socket.
When only few samples are put in the block place additional tubes in
the four corner positions. This is to evenly distribute the lid pressure
and prevents single tubes from excessive pressure. Use of few tubes
may result in damage of the tubes by excessive pressure.
Appropriate safety regulations must be observed when working with
infectious, pathogenic or radioactive material. Ask the responsible
local safety inspector for details.
The TProfessional Thermocycler must not be used with explosive,
flammable or volatile liquids.
Do not place fingers between lid and housing when opening or
closing the lid.
Before opening of the lid, release lid pressure (see section 4.5)
It is not necessary to apply oil into the opening of the block in order
to improve the heat transfer between the block and the sample
tubes.
If you still decide to use oil, do not use silicon oil. Mineral oil may be
used.
Ensure that both the rear and bottom ventilation slits not clogged by
dust or other material. Danger of overheating!
Let equilibrate the TProfessional Thermocycler to room temperature
before starting operation.
There must be sufficient distance between the ventilation slots on the
rear side of the Thermocycler and a wall or another instrument (min
10 cm). Danger of overheating!
Instruction Manual TProfessional 07/2009
11
This instrument is designed and certified to meet EN 61010-1 safety
standards. It should not be modified or altered in any way. Alteration
of this instrument will void the warranty, void the EN61010-1
certification, and create a potential safety hazard.
Place the TProfessional Thermocycler on a stable, non flammable
surface in a dry, safe environment. For details see working
conditions in table “Technical specifications” (see chapter 1.3).
Do not use alcohol (e.g. methanol, ethanol), organic solvents or
abrasives to clean the instrument.
For transports always use the original Biometra box.
12
Instruction Manual TProfessional 07/2009
3
Installation
3.1 Content of delivery
1)
2)
3)
4)
Thermocycler with exchangeable block module
Mains connector
Manual
Short Manual
Please keep the original packaging material for return shipment in case of servicing. The
TProfessional shipping box provides a specially developed system for contact-free transport of
this electronic device.
3.2 Unpack and Check
Unpack and carefully examine the instrument. Report any damage to Biometra. Do not attempt
to operate this device if physical damage is present.
Please keep the original packing material for return shipment in case of service issues
!! Attention !!
Please fill out and send back the warranty registration card. This is important for you to
claim full warranty.
3.3 Installation Conditions
•
Place the TProfessional Thermocycler on a stable surface in a dry, safe environment. For
details see working conditions in table “Technical specifications” (see chapter 1.3).
•
Let equilibrate the TProfessional Thermocycler to room temperature before starting
operation (1 to 6h).
•
Make sure that the appliance connector and the plug of the supply cord are accessible, so
you can separate the instrument from the mains.
•
Make sure that the ventilation slots on the bottom and the rear are not obstructed (see
section 4.2). Make sure that there is no object underneath the thermocycler that may block
the ventilation slots at the bottom (e.g. a piece of paper etc.)
•
There must be sufficient distance between the ventilation slots on the rear side of the
Thermocycler and a wall or another instrument (min 10 cm).
Instruction Manual TProfessional 07/2009
13
Ensure that both the rear and bottom ventilation slits of the
rear and bottom of the instrument are unobstructed.
Insufficient ventilation can cause overheating of the
instrument.
•
Make sure that the main supply voltage is in accordance with the label above the power
connection (see section 4.2)
•
Connect the TProfessional Thermocycler to a grounded socket.
Prior to connecting the unit to the power source please ensure that the
voltage selector at the back side of the instrument is set to the required
voltage.
Danger of electric shock! Unplug the power cable before you open the
TProfessional Thermocycler.
•
The display contrast can be adjusted to local light conditions (6.2.2)
3.4 Operation Voltage
Important: Prior to connecting the TProfessional to the mains, make sure that the setting of the
Voltage selector is in accordance with your mains Voltage.
The TProfessional Thermocycler can operate at 100, 115 or 230 Volt. The operation Voltage is
shown on the Voltage selector which is located at the instrument bottom.
To change operation Voltage of the TProfessional, switch off the instrument and disconnect the
mains plug.
Use a coin or another round shape item to turn the adjustment slot of the Voltage selector to the
new Voltage.
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Instruction Manual TProfessional 07/2009
Instrument bottom
Instrument backside
Voltage
indicator
Mains plug
230
11
5
Fuses
110
Fuses
Adjustment
slot
1
Power switch
2
3.5 Initial self test (power on self test)
After switching on the TProfessional serial number of the instrument and the software version is
displayed.
A log file of the power on self test is stored in the Thermocycler memory (see section 6.4.1).
Instruction Manual TProfessional 07/2009
15
4
Operating elements
4.1 TProfessional Thermocycler front view
Lid pressure
adjustment wheel
Lid open
button
Display
Start / Stop
Button
Keypad
4.2 TProfessional Thermocycler rear view
R232 serial Port
Block release
lever
Mains plug
16
Power on / off
Instruction Manual TProfessional 07/2009
4.3 Exchange of block module
Switch off the instrument.
Lift release lever on the backside (1) then pull block slowly to the back (2).
2
1
To install a new block module insert module carefully from the back in the base unit (2) until the
plugs at the front of the module are in contact with the socket inside the instrument. Then
carefully push down the release lever (1).
Switch on instrument. During boot up the block module (block format and serial number) is
automatically recognized.
4.4 The TProfessional control panel
Menue Buttons
Keypad
LED
Block
status
Start
Stop
Back
Instruction Manual TProfessional 07/2009
Soft Keys
Navigation
17
4.5 High Performance Smart Lid (HPSL)
To achieve optimum pressure on the tubes the TProfessional is equipped with a height
adjustable heated lid.
Close the lid:
After the samples have been placed in the block close the lid. Turn the wheel clockwise until
you hear a clicking noise. In this mode the pressure will not increase further, even when you
keep on turning the wheel.
Note: The pressure of the lid has been optimized for a fully loaded block. If only very few tubes
are loaded to the block you should place two dummy tubes each in the four corner positions to
avoid damage of tubes by excessive pressure.
Open the lid:
First: Release pressure by turning the wheel counter clockwise. As soon as there is no more
resistance the pressure has been released.
Then: Open the lid with by pushing the front button.
Important: The lid should not be opened under pressure because this leads to damage of the
locking mechanism.
18
Instruction Manual TProfessional 07/2009
5
Operating
5.1 The TProfessional User Interface
The TProfessional User interface provides four Menu Buttons above the screen and four Soft
Keys below the Screen,
Menu buttons
Softkeys
5.1.1
TProfessional Menu Buttons
The four MENU BUTTONS allow quick access to the TProfessional Main Menus. These are:
•
•
•
•
Login /out Menu
System
Menu
Block
Menu
Program
Menu
The active menu is indicated by a graphical link to the respective menu button.
The example shows Program Menu is active.
The four Menu Buttons are permanent and always have the same function. This is in contrast
to the Soft Keys. The Soft Key functions change depending on the current software menu.
5.1.2
TProfessional Soft Keys
Below the Display there are four SOFT KEYS.
Instruction Manual TProfessional 07/2009
19
In contrast to the Menu Buttons, the Soft Keys have changing functionalities, which are shown
in the above display line.
5.1.3
Log in Menu
To enter the Log In menu, press the Menu Button [Log in/out] above the display.
When the instrument is switched on, the Log in screen is displayed. To use the instrument a
user has to be selected. Alternatively, a new user can be created.
5.1.4
Block Menu
To enter the Block Menu, press the Menu Button [Block] above the display.
20
Instruction Manual TProfessional 07/2009
The block menu shows the current status of the block. The block can be active with a program
or free.
If the block is free (not running), the five most recently used programs (by the active User) are
listed. These programs can be started directly from the block menu (select with cursor and push
[start] Button).
5.1.5
System Menu
To enter the System Menu, press the System Menu Button above the display.
The System Menu allows global settings and provides information about the instrument. The
power on self test log files can be retrieved and service files generated, a detailed description of
this menu is shown in section 6, page 51.
5.1.6
Program Menu
To enter the Program Menu, press the Program Button above the Display.
Instruction Manual TProfessional 07/2009
21
In the Program Menu, existing programs can be edited or new programs can be created. Each
User has his own program directory, where individual programs are saved. Programs from other
user directories can be used, but not modified.
5.2 TProfessional software
5.2.1
Navigation within software menus
Four navigation keys provide easy navigation within the software screens. The [left] and the
[right] key have additional functionalities as described below:
The right cursor key moves the cursor to the next field.
This cursor can also be used to confirm data entry. By pressing the right cursor
settings will be saved and the cursor moves to the next field.
In the file directory, this key moves to cursor forward to the next (lower) level.
The left cursor key moves the cursor back to the previous field.
In most screens this cursor is equivalent to the ”back” key.
In the file directory, this key moves the cursor back to the higher level.
5.2.2
Log in
The TProfessional manages up to 30 individual users. For each user a directory is created,
where programs are stored. Programs from other directories (i.e. users) can be read but not
modified. This allows to use all programs that are in the cycler memory, but to write only in the
own user directory.
Each User is identified by User Name plus Initials (2-3 letters).
Each User account can be protected by a PIN consisting of 1-7 digits. If no PIN protection is
wanted, leave the field “Enter PIN” empty and confirm with [Enter].
Note: If no PIN protection is used, programs in the User directory may be changed or deleted
by unauthorized persons.
5.2.3
Log in existing User
Press Menu Button [Log in/out] above the Display. A list of Users is shown
22
Instruction Manual TProfessional 07/2009
Select User with cursor keys, or enter first initial. The cursor jumps to the first account starting
with this letter.
Press Soft Key [log in] and enter your PIN.
Instruction Manual TProfessional 07/2009
23
Confirm PIN with button [PIN code ok]. The current User is shown:
To proceed with editing or running a program, select one of the Menu Buttons above the display
5.2.4
Create new user account
Press Menu Button [Log in/out] above the Display.
To create a new User account, press Soft Key [New User].
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Enter your name and 2-3 initials. Enter a personal identification number (PIN) consisting of 1-7
digits. For security reasons, you are requested to repeat the entry of your PIN.
Note: PIN entry is optional. If no password protection is wanted, just confirm with [User data
OK]. However, if no PIN is entered, you account containing all of your programs can be
modified by unauthorized personal.
Note: Your PIN can be changed any time in the System menu (see section 6.1.2).
5.2.5
Supervisor Log in
Only the Supervisor is authorized to change global instrument settings. This includes update of
firmware and the deletion of user accounts.
To log in as Supervisor, press the Log in/out Menu Button above the Display. Press Soft Key
[Supervisor Log in] and enter Supervisor PIN.
The default Supervisor PIN is 000 000 0 (do not enter blanks).
5.2.6
Delete User
Once logged in, you can delete your own account in the System Menu (see section 13.1.3.).
In addition, any user can be deleted by the Supervisor. To delete a user, log in as Supervisor
(see section 6.3.) and select menu option “delete user”. Choose the user account you would like
to delete and confirm with [OK].
Note: By deleting a User Account all programs in the referring user directory will be deleted. It is
therefore recommended to safe the programs in a different directory prior to deleting a User
account.
5.3 Create program
At Thermocyclers of the TProfessional series programs can be created in two ways. You may
use pre-installed program examples (see chapter 5.3.1) or create new programs without a
template (see chapter 5.3.2).
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5.3.1
Create program from template
Log in to your personal user directory and press menu button [Program] above the display to
enter the TProfessional program menu. In the directory “Biometra Shared” you will find several
pre-installed protocols for different PCR applications.
Press softkey [Open directory] to open the directory „Biometra Shared“. Choose a suitable
program using the cursor keys or enter the corresponding program number. Protocols may be
started directly from the Biometra Shared directory or may be copied to the respective user
directory and edited (see chapter 5.3)
Note: Please always, before starting a template protocol, check the single steps and adapt
details (times, temperatures, loops, temperature- and time-increments) according to the
required PCR conditions respectively. Biometra will not be liable if the PCR does not work if
necessary adjustments have not been programmed.
Note: The directory is protected by a PIN-Code. The default PIN for the directory “Biometra
Shared” is 0000.
5.3.2
Create program without template
Press menu button [Program] above the display (see chapter 5.3.1) to enter the TProfessional
program menu.
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Press softkey [Edit Program] and in the next screen press [New Program].
The programming table opens (see chapter 5.3.3).
5.3.3
Set block type
The TProfessional Thermocycler automatically recognizes the installed block module. This
module is the default block type for creating a new program.
However, it is also possible to write a program for an alternative block type (i.e. w/o gradient).
The block type can thus be set manually by pressing the Soft Key [block Type].
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To create a program for a block type other than the one currently installed, please select one of
the listed block types.
5.3.4
Set lid pre-heating mode
You can choose whether the lid is pre-heated before the program starts. This is to avoid
evaporation during the initial heating phase.
Note: During the preheating of the lid, the block is held constant at 25°C.
Note: After reaching the set lid temperature, there is a 40 second equilibration phase before the
block starts.
Note: The default setting, which is recommended for most applications, is preheating: On.
However, if the program should start while the lid is heating, this can be set by pressing the Soft
Key [preheat on/off].
After Lid settings have been made, confirm with [Enter] to enter the programming spreadsheet.
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5.3.5
Set lid temperature
Note: Thanks to the design of the new High Performance Smart Lid, lower lid temperatures can
be used for thermocycling than in the past. Thanks to the lower lid temperature a higher
temperature uniformity between samples can be achieved.
The recommended lid temperature is 99.0°C.
Enter temperature and confirm with [Enter] or step to the next field with cursor key.
5.3.6
Enter step temperature and time
Use cursor keys to navigate in the programming table.
Note: Each setting is confirmed with [Enter]. The cursor moves automatically to the next field.
Alternatively, you can confirm a value by moving forward with the cursor keys.
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Enter temperature for the first step and press [Enter]. In the next row you can enter the time for
this temperature:
Note: Minutes and seconds can be separated by “dot”, but this is optional. You can also enter
the digits one after the other.
Example: To set 2 minutes, 30 seconds enter “2”, “3”, “0”. Subsequently 2:30 [m:s] will be
displayed in the programming table
Note: For Biometra Thermocyclers a dot can be used as a shortcut to program minutes.
Example: To set 2 minutes enter “2”, “.”. Subsequently 2:00 [m:s] will be displayed in the
programming table
5.3.7
Set loop
Note: In general, loops are entered in the LAST STEP of the loop. A loop is defined by
selecting the FIRST STEP in the loop plus the number of back loops.
Enter the number of the first step within the loop in the row “go to” and confirm with [Enter].
Enter the number of back loops in the row “loops”.
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The loop is shown as a bracket at the left side of the spreadsheet.
Note: Total cycler number = (number of back loops) + 1, e.g. enter 24 for a total cycle number
of 25.
5.3.8
Cool below ambient temperature
After the program has been finished, samples can be kept at below ambient temperature.
Note: To save lifetime of Peltier elements, a temperature of 15°C for the final step rather than
4°C is recommended.
5.3.9
Program Pause
To hold a temperature for an indefinite time enter “0”. After confirming with [Enter] the word
“pause” is shown.
5.3.10 Save program
Press button [Save / Save as].
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The first available program number is automatically pre-selected. Use cursor keys (or enter two
digits) to move to a different program store.
To enter a program name press button [edit name].
Enter program name and confirm name with button [ok].
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Confirm program store number with Soft Key [Save].
Program number, name and date are shown in the file directory.
To run this program press Menu Button [block] above the display.
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Press the (illuminated) [Start] Button on the left side of the keypad.
5.3.11 Set Gradient
The easiest way to define a gradient is to enter the two temperatures of the block (left and right
side) separated by “-“.
Confirm with [Enter] or move cursor to the next field.
Alternatively, move cursor to the field for temperature setting and press Soft Key [Gradient].
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In the Gradient Screen you can enter a new, or edit an existing Gradient. Enter new settings for
Row01 (left side) and Row12 (right side) and confirm with Enter.
After both settings are confirmed with [Enter] the temperatures for the single rows are displayed
in the bar chart.
With the cursor keys UP and DOWN the gradient can be fine adjusted. Press
the cursor keys to increase or decrase the temperature in increments of 0.1 °C.
To go back to the spreadsheet, press Soft Key [Table].
5.3.12 Program linear gradient
Press softkey [Edit linear gradient].
Enter the desired annealing temperature and define a temperature increment (Incr.) between
the single rows of the heating block (E.g. the entered annealing temperature corresponds to the
temperature in row 6 of a 96 well block). The temperatures for all other rows are calculated
automatically. The temperature in row 01 (left side of the block) is the lowest and the
temperature in row 12 (right side of the block) is the highest.
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5.4 Edit programs
Programs can be edited in the Table or Graphical mode [Graph]. To change between these two
modes click on the Soft Key [Table] or [Graph] respectively. The graphical mode is intended for
easy modification of existing programs, which can then be saved under a new program number
and name. To edit programs in the graphical mode see section 5.4.5, page 41.
Note: During operation of the Thermocycler the active program can be viewed but not modified.
If you want to change settings of the active program for further experiments you have to save a
copy of this program to another memory. For further information about copying programs see
section 5.4.3, page 39.
5.4.1
Insert program step
Move cursor to the position, where a new step should be inserted
To insert a program step press Soft Key [Insert/Del]. The step at which a new step will be
inserted is highlighted.
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Press Soft Key [insert step].
Confirm with Soft Key [Yes]. Enter temperature and time settings for new step.
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Note: The new step has been entered within an existing loop, this loop now consists of four
steps.
5.4.2
Delete program step
Move cursor to the step that should be deleted and press Soft Key [insert/delete step].
To delete the highlighted step press Soft Key [Insert/Del]
Confirm deletion of step number 4 with Soft Key [YES]
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The total step number is reduced by one, the loop now contains 3 steps only.
5.4.3
Copy program
Press Menu Button [Program] and enter User directory.
Move cursor to the program you want to copy.
Press Soft Key [Copy Program]. The cursor automatically jumps to the next available (free)
program store.
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39
.
The program copy can be stored at any storage number. To change program number move the
cursor to the wanted location or directly enter a storage number (two digits) over the keypad.
Edit name and confirm with Soft Key [Save Copy]
5.4.4
Delete program
Select a program from your user directory
Press Soft Key [Delete program].
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Instruction Manual TProfessional 07/2009
Confirm Deletion with Soft Key [Yes].
This program store is now free.
5.4.5
Editing programs in the graphical mode
Programs are created in the spread sheet mode where new steps are easily defined by
Temperature and Time. In addition, programs can be edited in the graphical mode which
provides a schematic temperature plot of the different steps.
To enter this mode, press Soft Key [Graph].
Spread Sheet Mode [Table]
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Graphical Mode [Graph]
41
To change temperature and time settings, move cursor to the respective field and enter new
values with the key pad. Once confirmed with enter, the temperature graph is updated.
Note: The steps are automatically spread over the whole range to give maximum resolution
(thus steps height does not represent the real temperature).
Note: To set loops, go to the Spread Sheet Mode (press [Table])
Note: To insert or delete program steps, go to the Spread Sheet Mode (press [Table])
Note: To edit or enter gradients, move cursor to the respective step and press Soft Key
[Gradient]. Enter gradient temperatures as described in section 5.3.11, page 34).
5.5 Change program options
For each step additional options can be defined.
To change program options, move the cursor to the right side of the spreadsheet, labelled with
“Options”. A new table is displayed.
To go back to programming sheet, move the cursor to the left side of the display
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If options have been changed from the default values, there is an indication in the options field.
5.5.1
Heating and cooling rate
Since the TProfessional is a very fast thermocycler it may be necessary to reduce the heating
and cooling ramp to adopt protocols from slower thermocyclers.
The average heating or cooling rates can be set in the column labelled with Ê[°C/s].
Note: This entry specifies the speed at which the referring step is achieved. I.e. to heat from
step 1 to step 2, the heating rate in step 2 has to be lowered.
Note: To reduce the over all speed of the Thermocycler, the heating rate has to be modified in
all steps. The speed settings will be only valid for this individual program.
5.5.2
Time increment Æ Δt(s)
To compensate for loss in enzyme activity, each step within a loop can be extended from cycle
to cycle. Enter the desired time increment [seconds] in the row labelled with dt[s]. This value will
be added to the time value from cycle to cycle.
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Note: A time increment will increase the total runtime depending on the numbers of cycles and
the size of the increment. A program with many cycles and large time increments will take a
significantly longer time than a standard protocol.
5.5.3
Temperature increment or decrement Æ ΔT(°C)
For some applications it is useful to start with a higher temperature and to decrease this
temperature from cycle to cycle. This subsequent lowering of a temperature is called touch
down.
To decrease a temperature from cycle to cycle enter a negative temperature increment in the
row labelled with ΔT[°C].
Note: Be sure that the temperature decrease is set in a step that lies within a loop. Otherwise
there will be no iterative temperature decrease.
5.6 Run program
To start program press [Start] Button (see section 4.4, page 17).
This button is only active, when a program for start has been selected. This is indicated by the
green LED in the Start button.
5.6.1
Select program for start from User directories
Any program in the cycler memory can be used to start the thermocycler. In addition to the
programs in the own User account, also programs from other User directories can be started.
To select a program for start press [Program] Menu Button above the Display.
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Select directory and program with cursor keys and press [Start] Button.
5.6.2
Quick start programs from the block Menu
The TProfessional provides the last 5 programs that haven been run for quick start.
Push the [block] Menu Button above the Display.
Select one of the last five programs with the cursor keys.
Press the (illuminated) Start button on the left side of the keypad (see section 4.4, page 17).
5.6.3
View program prior to start
To check the program prior to start press the [View Program] Soft Key.
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45
The current program is shown.
Press [View Graph] Soft Key for alternative display.
View Table
View Graph
Note: In the view program mode, a program cannot be edited. To edit program press [Program]
Menu Button above the Display.
5.6.4
Display during operation
After the instrument has been started, the below display is shown
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To show detailed information about the running program, press one of the Soft Keys [Show
Graph] and [Show Table].
There are three different display modes available during the run.
Show Time
5.6.5
Show Graph
Show Table
Pause Program during run
To pause program press Soft Key [Pause Block].
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The message “PAUSE” is shown in the display and alternates with the elapsed time.
5.6.6
Continue program
To continue paused program press Soft Key [Continue]
5.7 Stop program
To stop the active program press [stop] Button.
To abort the active program confirm with [Yes].
5.7.1
Stop Program from a pause
If the last step in a program is a pause, the program has to be stopped by pressing the [Stop]
Button before switching off the instrument.
Note: If the instrument is switched off during a pause step, this will be recognized as a power
failure (a referring error message is written to the log file).
5.8 Optimization of Fast-PCR programs
The usage of Fast-PCR Kits allows a significant shortening of PCR protocols in comparison to
normal programs when conventional polymerases are used. Though suppliers of Fast-PCR kits
suggest some protocols for their polymerases for the optimization of Fast-PCR protocols some
physical factors have to be considered. E.g. the temperature transfer from the thermocycler
block into the reaction mix is hindered by low temperature conductivity of the plastic of the
reaction tubes. Moreover the temperature spread within the block, especially at short dwells,
depends on its geometry and is different between various block types. To reach the desired
temperature in the reaction tube, when dwells are shortened ( 15 sec), the target temperature
of program steps has to be adapted, otherwise drop-outs due to insufficient denaturation or
insufficient primer annealing may occur. Biometra recommends the following protocol templates
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for the optimization of dwells for the TProfessional 96 and TProfessional Standard 96
Thermocycler:
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49
Dwell 15sec, Annealing temperature 60 °C
95 °C
5:00
96 °C
00:15
59 °C
00:15
35x
68 °C
00:30
72 °C
01:00
16 °C
Pause
Dwell 5sec, Annealing temperature 60 °C
95 °C
5:00
97,5 °C
00:05
57,5 °C
00:05
35x
68 °C
00:30
72 °C
01:00
16 °C
Pause
Dwell 10sec, Annealing temperature 60 °C
95 °C
5:00
96,5 °C
00:10
58,5 °C
00:10
35x
68 °C
00:30
72 °C
01:00
16 °C
Pause
Dwell 1sec, Annealing temperature 60 °C
95 °C
5:00
99 °C
00:01
56 °C
00:01
35x
68 °C
00:30
72 °C
01:00
16 °C
Pause
The protocol templates are pre-installed in the directory “Biometra Shared” (see chapter 5.3.1).
Note: The given annealing temperatures correspond to a calculated primer annealing
temperature of 60°C. If necessary, the annealing temperature has to be optimised for each
single primer pair. Biometra recommends to optimize the annealing temperature by a gradient
at 0.5 °C steps on a gradient enabled PCR Thermocycler.
Note: For the protocol templates an elongation time of 30 sec each is given. For optimisation of
the elongation time please take the processivity (bases/sec) of the Fast-PCR polymerase given
in the supplier handbook into consideration and adjust the elongation time corresponding to the
length of the fragment to be amplified. At a processivity of 35 bp/sec the elongation time given
in the protocol templates would be sufficient for the amplification of a 1000 bp long DNA
fragment.
Note: The proposed protocols are only applicable for the Thermocylers TProfessional 96 and
TProfessional Standard 96. To establish a Fast-PCR at the Thermocycler TProfessional Basic
96 please follow the instructions in the homologue chapter of the corresponding manual.
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6
System settings
To change global settings press the [System] Menu Button above the screen.
Main screen
6.1 User configuration
This option allows changes of your individual user account.
6.1.1
Set language
The language settings are part of each User account. Available languages are English and
German (second software release)
6.1.2
Change individual PIN
The option allows changing the PIN of the current User.
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6.1.3
Delete Account
Any user can delete his or her personal account.
Important: Deleting a User account will automatically delete the user file directory together with
all programs stored in this directory.
Prior to deleting an account, ensure that the User directory is empty, or save a copy of the
programs for further use to a different User directory (see section 5.4.3).
6.2 System configuration
This option allows setting of the signal tone (beep), Display contrast and time settings.
6.2.1
Configure beep
To switch beep on/off toggle between ON and OFF and confirm with Soft Key [Set Beep].
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6.2.2
Configure Contrast
The Display contrast can be set individually for each User.
Change settings with Cursor keys and confirm with Soft Key [Save Contrast].
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6.2.3
Set time and date
Enter settings for Date and Time and confirm with Soft Key [Set Time and Date].
6.2.4
Defragment memory
By erasing and creating PCR-protocols after a while new files may be stored fragmented in the
memory of the TProfessional Thermocycler. This leads to increased memory access times and
the software reacts slower to user inputs. By defragmentation the information becomes sorted
and is stored in order thus leading to decreased memory access times. Subsequently the
software reacts faster to user inputs.
Confirm your selection with [OK] and wait until the defragmentation process is finished.
6.2.5
54
Screen saver
Instruction Manual TProfessional 07/2009
Set the screen saver ON or OFF and if necessary change the time after that the screen saver is
started (Standard time is 30 min.) Press [Save] to save the changings.
6.3 System info
This Menu provides information on the instrument and on the Log files of the last five runs.
6.3.1
System info
Use this option to Display the instruments serial number, block type and software version.
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55
6.3.2
View Log files of the last five runs
During each run a log file is stored in the Thermocycler memory. The log files of the last five
runs can be viewed in this window.
6.4 Service
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6.4.1
View log files of Power on self test
During boot up (Power on) the TProfessional hard ware is checked (Self test). Here you can see
the results of the last five self tests.
6.4.2
Execute extended self test
In addition to the regular Power on –Self test, a more comprehensive test can be triggered by
the user. In the extended block self test the unit can check the status and condition of different
components and functions. Therefore, this test is an important and useful information to the
customer whether the Thermocycler is still working within its parameters.
The extended block self test is listed in the [System] Menu under [Service]. With [Execute
Selftest] this test is initiated. This test takes several minutes. During the test no programs can
be run on the thermocycler.
In certain intervals the unit will automatically recommend to check the components of the unit,
i.e. to execute a new extended block self test (message 729).
Pressing [Quit] confirms that this recommendation has been noted. In case the unit is switched
off but the extended block self test has not been initiated, this recommendation window will
appear again if the unit is switched on.
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It is recommended to execute an extended self test regularly.
6.4.3
View log files from extended self test
The results of the extended self test logfiles are stored by the instrument. To view a summary of
the self test select the suitable logfile from the menu.
In the next window the actual status of all important components is summarized at a glance.
6.4.4
View Error log files
A history of all software and hardware errors is shown in this menu.
Note: Sever errors that require action by the User are displayed in the User Software Menu.
6.4.5
Create Info file for Biometra Service Department
This option creates a Service file containing technical details for the Biometra Service
Department.
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6.5 How to contact Biometra
6.6 Supervisor system configuration
The supervisor has access to an own system menu with some specific functions.
6.6.1
Change user PIN
The supervisor can change the PIN for each user account. Select the appropiate user directory
from the list and enter a new PIN.
6.6.2
Delete user account
Select the user directory to delete from the list and confirm with [OK]. The directory and the
programs therein are deleted.
6.6.3
Change supervisor PIN
Enter your actual PIN (the default Supervisor PIN is 000 000 0 (do not enter blanks)), enter your
new PIN and repeat your input. Confirm with [PIN OK].
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Note: Please make a note of the Supervisor Pin und keep it on a safe spot. A forgotten
Supervisor Pin can only be reset by Biometras Service Department and requires to send back
the instrument.
6.6.4
Set boot language
The language displayed during the boot process can be set by the supervisor.
6.6.5
Delete all user accounts
The supervisor can delete all user accounts and including all progams. In the next screen
confirm with [OK] to delete all accounts.
Note: Deleted user accounts and programs can not be reconstituted
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7
Short manual
Safety Warnings
Do not open the instrument unless you are authorised to do so. Check the
label at the backside if the instrument has correct voltage configuration (110V,
115 V or 230 V).
The thermoblock and the heated lid will reach high temperatures during
operation. Both thermoblock and heated lid can burn you.
Rapid heating of the thermoblock can cause liquids to boil explosively. Always
wear safety goggles during operation. Close the lid before starting a program.
It is not necessary to apply oil into the opening of the block in order to improve
the heat transfer between the block and the sample tubes. If you still decide to
use oil, do not use silicon oil. Mineral oil may be used.
Ensure that both the rear and bottom ventilation slits at the rear and bottom of
the instrument are unobstructed. Insufficient ventilation can cause overheating
of the instrument.
The TProfessional Control Panel
Menu Buttons
Numpad
LEDs on
Block
status
Start
Stop
Back
Soft Keys
Navigation
Log in
After the TProfessional has passed the initial self-diagnosis the login menu is displayed:
 Press [Log in] and log in as existing user
 Press [Supervisor Log in] and log in as supervisor
 Press [New User] and create new user account
The language displayed in the user interface can be selected (English or German). The selected
language will be stored for each individual user.
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 Press [Select Language] to select preferred language
Create, modify and save programs
 To create a new or edit an existing program, press the [Program] menu button.
 Move cursor to your directory and press [Open directory].
 Press [New Program] or choose existing program and press [Edit Program] to enter the program
screen.
Use the cursor keys to navigate in the program screen. In each case the activated field is displayed
inverted. Corresponding to the activated field the assignment of the Soft Keys in the TProfessional control
panel will change accordingly.
Note: In the directory “Biometra Shared” you will find pre-installed protocol templates for different PCR
applications. In chapter 5.3.1and 5.8 you will find instructions how to use these protocols.
The TProfessional program screen
Status display
„Block type“
Status display
„Preheat Lid“
Defined
cycles
Options tab
Programming table
 To alter block type activate the corresponding status display and press [Block Type].
Select appropriate block type from the menu in the next window. (The TProfessional Thermocyclers
automatically recognize the installed block module. However, it is also possible to write a program for
an alternative block type.)
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 To switch lid preheating on or off activate the corresponding status display and press [Preheat
ON/OFF].
Set lid temperature in status display under [-- °C] within the range of 30°C to 99°C.
 Enter temperature [°C] and time [m:s] in the programming table for each single step. To hold a
temperature for an indefinite time enter “0” under [m:s] and press <enter>. Subsequently, the word
“pause” is shown in the display.
Note: Minutes and seconds can be separated by “dot”. You can also enter the digits one after the
other. Example: to set 2 minutes, 30 seconds enter “2”, “3”, “0” or “2”, “.”, “3”, “0”.
 To insert or delete a program step move cursor to the corresponding position in the programming table
and press [Insert/Delete Step].
 To insert or delete a program step press [Insert Step] or [Delete Step]. The position at which a
step will be inserted or deleted is highlighted.
 In the next screen confirm insertion or deletion with [YES]
 To define a loop A) enter the step the program shall go back to [goto] and B) enter how often the loop
is repeated [loops]. The defined loop is graphically displayed by a bracket at the left side of the
spreadsheet, the total cycle number is mentioned.
 To program special features like temperature increment, time increment or heating and cooling rate
repeatedly press the cursor key <right> to open the options tab. A new table is displayed:
Tip:
Storing samples at 16°C rather than 4°C at the
end of a run will increase the instruments life
time.
 To reduce or increase the annealing temperature from step to step within a loop set a negative or
positive temperature increment under ∆T(°C).
 To increase or reduce the incubation time from step to step in a loop set a positive or negative time
increment under ∆t(s).
 By default the ramping rate (heating and cooling) is pre-set to 5°C/sec. To adjust the ramping rate,
enter any value between 0.1°C and 5.0°C under Ê[°C/s].
 (For gradient enabled units only) To define a gradient A) enter the two temperatures of the block (left
and right side) separated by a minus under [°C] (e.g. 40-60) or B) press [Gradient] and then [Edit
linear Gradient]. This activates the screen for programming linear gradients:
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 Enter an annealing temperature. The set annealing temperature defines the temperature in row 6 of
the thermoblock. Enter a temperature increment [Incr.]. The corresponding temperature profile is
shown for each single row.
 Press [Save/Save as] to save a program.
Subsequently the program storage screen opens:
Tip:
A folder with common protocols could be
established. From the common folder each user
may copy any program into the own account and
modify it easily.
 By default the program is stored at the first free storage place available. To save a program at a
specific storage place use cursor keys <up> and <down> or enter two digits.
 To edit the name of the program press [Edit name]. In the next screen occurring enter a name by
using the keypad and confirm with [OK].
Start, pause and stop programs
 The TProfessional Thermocycler for each user stores the last 5 programs that have been run or
modified. For quick start of one of these programs press the [Block] menu button. In the next screen
select one of the last five programs by using the cursor keys <up> and <down>. Press the [Start]
button on the left side of the keypad to start the program.
 To start a program from a user directory log in with your user account and press the [Program] menu
button. In the next screen select the appropriate user directory and program by navigating with the
cursor keys. Press the [Start] button on the left side of the keypad to start the selected program.
After starting a program the following screen is shown:
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Instruction Manual TProfessional 07/2009
 To pause an active pogram press [Pause Block]. The message “PAUSE” is shown in the display and
alternates with the elapsed time.
 To continue a paused program press [Continue].
To stop an active program press the [Stop] button on the left side of the keypad and confirm with [OK] in
the TProfessional program screen. Programs with a pause in the last step have to be stopped manually
as well.
Instruction Manual TProfessional 07/2009
65
8
Trouble shooting
8.1 Slow heating and cooling
The TProfessional is equipped with a strong ventilator for the cooling of the heat sink (see also
chapter 9.1). The inlet of this fan is located at the bottom side of the instrument. Be sure that the
inlet is not clogged by dust or other material (e.g. a sheet of paper placed under the cycler can
be attached to the inlet as the fan is in operation). Dust can be removed easily from the inlet
with a conventional vacuum cleaner.
8.2 Autorestart
Running programs will be continued from the same step where the power failure has happened
as soon as the power is supplied again. A message is displayed showing (a) that a power
failure occurred during the run and (b) when and at which step the program was started again:
Confirm with [OK] to close the message screen.
8.3 Autorestart due to unrecognised power failure
High voltage fluctuation can lead to an automatic restart of the thermocycler. In this case the
cycler restarts at the step where there power failure has occurred. A message is displayed
showing (a) that a power failure occurred during the run and (b) when and at which step the
program was started again (see 8.2). To avoid voltage fluctuation, do not connect the cycler to a
socket shared by a strong power consumer like a refrigerator or a centrifuge.
8.4 Adaptation of protocols from other cyclers
Since the TProfessional is a fast instrument it may be necessary to reduce the heating and
cooling ramps to run protocols from other cyclers. For the setting of the heating and cooling
ramps see section 5.5.1. Alternatively, the time settings may be extended.
8.5 Releasing wheel in case of blocked lid
When the lid is in the very up or down position, it may happen that the wheel is uncoupled. In
this situation the clutch mechanism is active in both directions (clicking noise in either direction).
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Instruction Manual TProfessional 07/2009
To unlock wheel, press down metal pin with a ball pen and turn wheel carefully. This pin
overrides the automatic clutch mechanism. Thus, care must be taken not to apply excessive
pressure.
Release lid in upper position:
1) press pin
2) carefully turn wheel while holding
the pin down CLOCKWISE, until you
feel normal resistance (no more
clicking noise, clutch is released).
Release pin and turn lid down, until
the clutch mechanism is activated
(clicking noise, optimum pressure
applied).
1
2
Release lid in down position:
1) press pin
2) carefully turn wheel while holding
the pin down COUNTER
CLOCKWISE, until you feel normal
resistance (no more clicking noise,
clutch is released). Release pin and
turn wheel counter clockwise until
pressure is completely released.
Open lid.
Important: When the clutch mechanism is active (= optimum pressure is applied), do not use pin
to further increase lid pressure. This would lead to damage of tubes and instrument!
8.6 Recommendations for the use of 384 well Thermocyclers
a) Use the recommended 384 well plates and sealing materials
To prevent sample evaporation by insufficient sealing please use 384 well plates and sealings
tested and recommended by Biometra. A list of plates and sealing is available at
www.biometra.com -> thermocyclers -> plasticware
b) Reduce the temperature of the heated lid
Due to the fact that the heated lid is very close to the reaction volume with a 384 well plate
compared to 0.2 ml vessels the lid temperature can have a significant impact on the effective
incubation temperature. For reaction volumes less than 10 µl the lid temperature is
recommended to be set at 95 °C. When using a reaction volume of 10 µl or more, set lid
temperature at 85 °C. Even at these lowered lid temperatures possible condensation will not
occur as problem due to a mixture of physical factors like dew point, salt concentration,
temperature difference, air pressure within the tube and reaction volume. It is strongly
recommend to reduce the lid temperature according to the reaction volume. Otherwise, at
conventional lid temperature settings, sample volumes or amplification yields might be reduced.
Instruction Manual TProfessional 07/2009
67
9
Maintenance and repair
9.1 Cleaning and Maintenance
The TProfessional was built to operate for a long time without the need for periodical
maintenance. Nevertheless, occasionally cleaning of the air inlet may be necessary to maintain
the efficiency of the Thermocycler. Insufficient airflow may lead to reduced heating and cooling
rates (see chapter 8.1). The inlet for the airflow is located at the bottom side of the instrument.
Be sure that the inlet is not clogged by dust or other material (e.g. a sheet of paper placed
under the cycler can be attached to the inlet as the fan is in operation). Dust can be removed
easily from the inlet with a conventional vacuum cleaner. Additionally, the Thermocycler housing
may be cleaned from time to time with a smooth cotton cloth. Do not use strong detergents or
organic solvents for cleaning. Never treat silver block with abrasive agents.
Important: Appropriate safety regulations must be observed when working with infectious or
pathogenic material.
9.2 Servicing and repair
The TProfessional Thermocycler contains no user serviceable parts. Do not open the housing
instrument. Service and repair may only be carried out by the Biometra Service department or
otherwise qualified technical personal.
The Service department offers Thermocycler maintenances and temperature verifications.
Biometra recommends an annual maintenance and a biannual temperature check for all
Thermocyclers. Please call the following phone number for detailed information: +49 55150881-10/12.
9.3 Firmware update
For instruction for firmware upgrade, please contact the Biometra Service Department or your
local distributor/sales representative.
9.4 Replacement of Spare Parts
Only original spare parts mentioned in these operating instructions are allowed.
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Instruction Manual TProfessional 07/2009
10 Accessories
10.1 Block modules
Five different exchangeable block modules are available for the TProfessional Thermocycler:
Model
Order number
TProfessional Module Gradient 60
TProfessional Module Gradient 96
TProfessional Module 60
TProfessional Module 96
TProfessional Module 384
070-810
070-811
070-910
070-911
070-912
10.2 Plasticware
Biometra offers a broad range of plasticware for the use in PCR. The following table provides an
overview for the product portfolio:
Cat. no.
050-310
050-320
Description
Single tubes
0.2 ml tubes with caps
0.5 ml tubes with caps
1000 pcs.
1000 pcs.
050-254
050-255
Tube strips
Strips 8 tubes and flat caps
Strips 8 tubes and domed caps
125 pcs.
125 pcs.
050-225
48 well plates
48 well microplate
50 pcs.
050-232
050-213
050-253
96 well plates
96 well skirted
96 well non-skirted (low profile)
96 well non-skirted
25 pcs.
25 pcs.
25 pcs.
050-231
050-240
384 well plates
HSQ 384 well skirted
384 well microplate
50 pcs.
50 pcs.
050-237
050-256
050-257
050-236
050-194
050-694
050-251
050-252
Miscellaneous
Silcone mat
Adhesive film
Heat Sealing Film
Heat Sealing Film Aluminium
96 well aluminium plate
384 well aluminium plate
Nop mat 96 well
Nop mat 384 well
50 pcs.
100 pcs.
100 pcs.
10 pcs.
1 pcs.
1 pcs.
20 pcs.
20 pcs.
Instruction Manual TProfessional 07/2009
Quantity
69
11 Service
Should you have any problems with this unit, please contact our service department or your
local Biometra dealer:
Biometra GmbH
Service Department
Rudolf-Wissell-Straße 14 - 16
D-37079 Göttingen
Phone:++49 (0)5 51 50 68 6 - 10 or 12
Fax: ++49 (0)5 51 50 68 6 -11
e-mail: [email protected]
• If you would like to send the unit back to us, please read the following return
instructions in chapter 11.1.
•
11.1 Instructions for return shipment
In case of an instrument failure that cannot be fixed by the procedures described in section 8
please proceed as follows:
•
Return only defective devices. For technical problems which are not definitively recognisable
as device faults please contact the Technical Service Department at Biometra (Tel.: +49 55150881-10/12, Fax: +49 551-50881-11, e-mail: [email protected]).
•
Please contact our service department for providing a return authorization number (RAN).
This number has to be applied clearly visible to the outer box. Returns without the RAN will
be not be accepted!
•
Important: Carefully clean all parts of the instrument of biologically dangerous, chemical or
radioactive contaminants. If an instrument is contaminated, Biometra will be forced to refuse
to accept the device. The sender of the repair order will be held liable for possible losses
resulting from insufficient decontamination of the device.
•
Please prepare written confirmation that the device is free from biologically dangerous and
radioactive contaminants. The declaration of decontamination (see section 12) must be
attached to the outside of the packaging.
•
Use the original packing material. If not available, contact Biometra or your local distributor.
•
Label the outside of the box with “CAUTION! SENSITIVE ELECTRONIC INSTRUMENT!”
•
Please enclose a note which contains the following:
70
a)
Sender’s name and address,
b)
Name of a contact person for further inquiries with telephone number,
c)
Description of the fault, which also reveals during which procedures the fault occurred, if
possible
Instruction Manual TProfessional 07/2009
11.2 Packing of the Thermocycler
Biometra uses an extra designed packaging system where the instrument is mounted in
between two tearproof foils. The Thermocycler is put onto the lower inlet and is fixed in between
the foils by pressing the upper inlet down.
Note: The Thermocycler is only protected from transport damage if the packing instructions are
followed and the instrument is mounted in between the foils. Biometra will not be responsible for
transport damage by improper packing.
Gerät zwischen den
Folien schwebend
einspannen!
Mount instrument
floating in between
the foils!
Instruction Manual TProfessional 07/2009
71
12 Equipment Decontamination Certificate
To enable us to comply with german law (i.e. §71 StrlSchV, §17 GefStoffV and §19 ChemG)
and to avoid exposure to hazardous materials during handling or repair, please complete this
form, prior to the equipment leaving your laboratory.
COMPANY / INSTITUTE
__________________________________________________
ADDRESS
______________________________________________________________
PHONE NO
_________________________
E-MAIL
______________________________________________________________
EQUIPMENT
FAX NO_________________________
Model
______________
Serial No
______________
______________
______________
______________
______________
______________
______________
If on loan / evaluation Start Date: __________________
Finish Date __________________
Hazardous materials used with this equipment:
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
Method of cleaning / decontamination:
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
The equipment has been cleaned and decontaminated:
NAME __________________________________ POSITION _______________________
(HEAD OF DIV./ DEP./ INSTITUTE / COMPANY)
SIGNED ________________________________ DATE ____________________________
PLEASE RETURN THIS FORM TO BIOMETRA GMBH OR YOUR LOCAL BIOMETRA
DISTRIBUTOR TOGETHER WITH THE EQUIPMENT.
PLEASE ATTACH THIS CERTIFICATE OUTSIDE THE PACKAGING. INSTRUMENTS
WITHOUT THIS CERTIFICATE ATTACHED WILL BE RETURNED TO SENDER.
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Instruction Manual TProfessional 07/2009
General Information for Decontamination:
Please contact your responsible health & safety officer for details.
Use of radioactive substances:
Please contact your responsible person for details.
Use of genetically change organism or parts of those:
Please contact your responsible person for details.
Instruction Manual TProfessional 07/2009
73
13 Note for the disposal of electric / electronic waste.
Note
for disposal of electric / electronic waste
Hinweis
für die Entsorgung von Elektroaltgeräten
Renseignement
du traitement des déchets des appareils
électrique / électronique
This symbol (the crossed-out wheelie bin) means, that this product should be brought to the return and / or
separate systems available to end-users according to yours country regulations, when this product has
reached the end of its lifetime.
For details, please contact your local distributor!
This symbol applies only to the countries within the EEA*.
EEA = European Economics Area, comprising all EU-members plus Norway, Iceland and Liechtenstein.
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Dieses Symbol (die durchgestrichene Abfalltonne) bedeutet, dass dieses Produkt von der Firma Biometra für
eine kostenlose Entsorgung zurückgenommen wird. Dies gilt nur für Geräte, die innerhalb Deutschlands
gekauft worden sind.
Kontaktieren Sie für die Entsorgung bitte die Biometra Service-Abteilung!
Außerhalb Deutschlands wenden Sie sich bitte an den lokalen Händler.
Dieses Symbol gilt nur in Staaten des EWR*.
*EWR = Europäischer Wirtschaftsraum, umfasst die EU-Mitgliedsstaaten sowie Norwegen, Island und
Liechtenstein.
------------------------------------------------------------------------------------------------------------------------------------------------------------------------Cet symbol (conteneur à déchets barré d´une croix) signifie que le produit, en fin de vie, doit être retourné à
un des systèmes de collecte mis à la disposition des utilisateurs finaux en conséquence des régulations par
la loi de votre pays.
Pour des information additionel nous Vous demandons de contacter votre distributeur!
Cet symbole s´ápplique uniquement aux pays de l´EEE*.
EEE = Espace économique européen, qui regroupe les États membres de l´UE et la Norvège, Islande et le
Liechtenstein.
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Instruction Manual TProfessional 07/2009
14 Declaration of Conformity
EU – Konformitätserklärung
EC - Declaration of Conformity
Göttingen, February 2006
im Sinne der EG-Richtlinie über elektrische Betriebsmittel zur Verwendung innerhalb
bestimmter Spannungsgrenzen 73/23/EWG
following the EC directive about electrical equipment for use within certain limits of voltage
73/23/EEC
und / and
im Sinne der EG-Richtlinie für die elektromagnetische Verträglichkeit 89/336/EWG.
following the EC directive about the electromagnetic compatibility 89/336/EEC .
Hiermit erklären wir, dass folgende Thermocycler:
Herewith we declare that the following Thermocyclers:
Typen / types:
Best.-Nr. / Order No.:
TProfessional 60, TProfessional 96, TProfessional Gradient 60,
TProfessional Gradient 96, TProfessional 384
070-800, 070-801, 070-900, 070-901, 070-902
den grundlegenden Anforderungen der
corresponds to the basic requirements of
EG-Niederspannungsrichtlinie 73/23/EWG und der
EC low voltage directive 73/23/EEC and the
EG-Richtlinie über die elektromagnetische Verträglichkeit 89/336/EWG entsprechen.
EC directive about the electromagnetic compatibility 89/336/EEC .
Folgende harmonisierte Normen wurden angewandt:
The following harmonized standards have been used:
EN61326 : 2004
EN 61010-1 : 2001
EN 61010-2-010 : 2003
Instruction Manual TProfessional 07/2009
Formular 006, Ver. 03
Dr. Jürgen Otte
Quality Manager
75
15 Warranty
This Biometra instrument has been carefully build, inspected and quality controlled before
dispatch. Hereby Biometra warrants that this instrument conforms to the specifications given in
this manual. This warranty covers defects in materials or workmanship as described under the
following conditions:
This warranty is valid for 24 month from date of shipment to the customer from Biometra. This
warranty will not be extended to a third party without a written agreement of Biometra.
This warranty covers only the instrument and all original accessories delivered with the
instrument. This warranty is valid only if the instrument is operated as described in the manual.
Biometra will repair or replace each part which is returned and found to be defective. This
warranty does not apply to wear from normal use, failure to follow operating instructions,
negligence or to parts altered or abused.
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Instruction Manual TProfessional 07/2009
16 Subject Index
Autorestart................................................ 64
Beep ......................................................... 50
Block Menu............................................... 19
Block module
exchange.............................................. 16
Block type................................................. 26
Contact ..................................................... 57
Contrast.................................................... 51
Control panel ............................................ 16
Declaration of Conformity......................... 73
Decontamination Certificate ..................... 70
Defragmentation....................................... 52
Disposal.................................................... 72
Fast-PCR.................................................. 47
Firmware update ...................................... 66
Gradient.................................................... 33
linear Gradient...................................... 34
Heated lid ..................................... 17, 27, 28
Heated Lid ................................................ 64
Language ........................................... 49, 58
Log file................................................ 55, 56
Log in........................................................ 21
Log in Menu.............................................. 19
Maintenance............................................. 66
Operation Voltage .................................... 14
Packing..................................................... 69
PIN-Code...................................... 22, 49, 57
Plasticware ............................................... 67
Program
continue................................................ 47
cooling rate........................................... 42
copy...................................................... 38
create ................................................... 24
delete.................................................... 39
delete step............................................ 37
edit........................................................ 35
Gradient................................................ 33
graphical mode..................................... 40
heating rate .......................................... 42
Instruction Manual TProfessional 07/2009
insert step............................................. 35
linear Gradient...................................... 34
loop ...................................................... 29
pause ............................................. 30, 46
quick start............................................. 44
save...................................................... 30
spread sheet mode .............................. 40
start ...................................................... 43
stop ...................................................... 47
temperature increment ......................... 43
time increment...................................... 42
Program Menu ......................................... 20
Repair ...................................................... 66
Safety precautions ................................... 10
Screen saver............................................ 52
Self test
extended .............................................. 55
Power on self test................................. 55
Service ..................................................... 54
Service info file......................................... 56
Servicing .................................................. 66
Short manual............................................ 59
Start ......................................................... 43
Stop.......................................................... 47
Supervisor.......................................... 24, 57
System configuration ............................... 50
System info .............................................. 53
System menu ........................................... 20
System settings........................................ 49
Technical specifications ............................. 8
Time and Date ......................................... 52
User account
create ................................................... 23
delete ....................................... 24, 57, 58
User Account
Delete................................................... 50
User interface........................................... 18
Warranty .................................................. 74
77
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