Life Technologies Identifiler Direct PCR Amplification Kit User Guide

The Identifiler Direct PCR Amplification Kit is a short tandem repeat (STR) multiplex assay optimized to allow direct amplification of single-source samples including blood and buccal samples on treated paper substrates without the need for sample purification.

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Life Technologies Identifiler Direct PCR Amplification Kit User Guide | Manualzz

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Key features

  • Direct amplification of single-source samples
  • Blood and buccal samples on treated paper substrates
  • No need for sample purification
  • 15 autosomal STR loci
  • Sex-determining marker Amelogenin
  • Compatible with Applied Biosystems instruments
  • Multicomponent analysis for separating fluorescent dye colors

Frequently asked questions

The kit is optimized to amplify single-source blood and buccal samples on treated paper substrates without the need for sample purification.

The kit amplifies 15 autosomal STR loci and the sex-determining marker, Amelogenin.

The kit is designed for use with Applied Biosystems instruments such as the 3100/3100-Avant Genetic Analyzer, 3130/3130xl Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730 Genetic Analyzer, GeneAmp PCR System 9700, and Veriti 96-Well Thermal Cycler.

Multicomponent analysis is a process that separates the five different fluorescent dye colors into distinct spectral components, allowing for the simultaneous amplification and efficient separation of multiple loci with overlapping size ranges. The dyes in the Identifiler Direct Kit are 6-FAM™, VIC®, NED™, PET®, and LIZ®.
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