SureCycler 8800 Setup and User`s Guide

SureCycler 8800 Setup and User`s Guide
SureCycler 8800
Setup and User’s Guide
Version C3, June 2015
For Research Use Only. Not for use in diagnostic
procedures.
Agilent Technologies
Notices
© Agilent Technologies, Inc. 2015
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Manual Part Number
G8800-90000
Edition
Version C3, June 2015
Printed in USA
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trademark of Microsoft Corporation in the
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Technical Support
For technical support, contact Agilent at
[email protected] Complete contact information for Technical Support is
listed on page 14.
Agilent SureCycler 8800
Manufactured by
Agilent Technologies LDA Malaysia Sdn. Bhd.
Bayan Lepas Free Industrial Zone
11900 Penang, Malaysia
For
Agilent Technologies Singapore (International) Pte. Ltd.
No. 1, Yishun Avenue 7,
Singapore 768923
www.agilent.com
2
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adhered to, could result in damage
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data. Do not proceed beyond a
CAUTION notice until the indicated
conditions are fully understood and
met.
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A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
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in personal injury or death. Do not
proceed beyond a WARNING
notice until the indicated conditions are fully understood and
met.
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Agilent SureCycler 8800 Setup and User’s Guide
South Korean Class A EMC Declaration
A 급 기기 ( 업무용 방송통신기자재 )
This equipment is Class A suitable for professional use and is for use in
electromagnetic environments outside of the home.
이 기기는 업무용 (A 급 ) 전자파적합기기로서 판 매자 또는 사용자는 이 점을 주
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Document Part Number: 5061-7383
*5061-7383*
© Agilent Technologies 2015
Agilent Technologies
In this Guide...
This document describes how to program and use the
Agilent SureCycler 8800.
1
Before You Begin
This chapter contains information for you to read and
understand before you start.
2
Installation
This chapter contains installation instructions for setting up
the cycler instrument.
3
Getting Started
This chapter contains instructions to load samples, get
started with the software, set up user accounts and access
instruments remotely via a network.
4
PCR Programs
This chapter contains instructions to work with PCR
programs, incubations and reports. Troubleshooting
suggestions are also provided.
5
Maintenance and Updates
This chapter contains instructions for maintaining the
instrument and uploading software updates.
4
Agilent SureCycler 8800 Setup and User’s Guide
Contents
1
Before You Begin
9
Materials Provided 10
Safety Precautions 10
Indicator LED Light 13
Overview 13
SureCycler 8800 Technical Support
2
Installation
14
15
Installation 16
Step 1. Select a location for the instrument 16
Step 2. Unpack the cycler 16
Step 3. Install the thermal block on the instrument base
Step 4. Connect the instrument to a power supply 17
Step 5. Connect optional devices 17
Step 6. Turn on the instrument 17
Step 7. Connect the cycler to a network (optional) 17
16
Thermal Block Exchange 18
To remove the thermal block 18
To install the thermal block 18
3
Getting Started
19
Sample Preparation and Loading 20
To prepare samples 20
To load samples 20
Agilent Plasticware and Consumables
Starting Up the Software
22
Touchscreen Operation of the Software
Agilent SureCycler 8800 Setup and User’s Guide
21
22
5
Contents
Home Screen
22
User Accounts 24
Access Levels 24
Logging In to User Accounts 24
Managing User Accounts 26
Network Settings and Remote Access 29
To view or edit the cycler’s IP address and name
To access the cycler from another cycler 30
To access the cycler from a computer 31
To access the cycler from an iPhone 31
29
Cycler Settings 32
Hot Top Setting 32
Cycler name 33
Time and date 33
4
PCR Programs
35
Creating New PCR Programs
36
To manually create programs 36
To create a PCR program using the PCR Wizard 39
Tips for Creating PCR Programs for the 384-well SureCycler 8800
Program Steps in the Commands List
Hot Start 43
Cycle Wizard 44
Start Cycle 45
Temperature Step 46
End Cycle 47
Gradient Step 47
Touchdown 49
Storage/Pause Step 50
43
Using a Preloaded PCR Program 51
To access the preloaded programs
To edit a preloaded program 52
6
42
52
Agilent SureCycler 8800 Setup and User’s Guide
Contents
Editing PCR Programs 53
To edit program step parameters
To copy a PCR step 54
To add or delete a PCR step 54
To delete a PCR program 54
Running a PCR Program 55
To run a PCR program 55
To stop or pause a program
53
55
Running an Incubation 56
To set up and run an incubation
56
Viewing Reports 57
To view reports 57
To delete a report 57
Troubleshooting 58
If PCR yield is low when running small reaction volumes with the Standard
ramp rate 58
If no actual temperature is displayed during the run 58
If the program does not start 58
If condensation is found in tubes during a run 59
If the sealed microplate loses sample volume 59
If USB devices including memory stick and keyboard not recognized 59
If cycler does not turn on 59
If screen is blank and unlit 60
If screen is lit but shows no information or has missing characters 60
60
5
Maintenance and Updates
Maintenance 62
Cleaning 62
Replacing a fuse
Software Updates
61
62
63
Agilent SureCycler 8800 Setup and User’s Guide
7
Contents
8
Agilent SureCycler 8800 Setup and User’s Guide
SureCycler 8800
Setup and User’s Guide
1
Before You Begin
Materials Provided 10
Safety Precautions 10
Indicator LED Light 13
Overview 13
SureCycler 8800 Technical Support 14
This chapter contains information for you to read and understand before
you start.
For installation instructions, see “Installation” on page 16.
Agilent Technologies
9
1
Before You Begin
Materials Provided
Materials Provided
Table 1
Materials Provided
Part
Quantity
SureCycler 8800 instrument base
1
Thermal block unit (96-well or 384-well)*
1
Power cord
1
USB external drive**
1
Functional test certificate
1
Quick Start installation poster
1
CD with Setup and User’s Guide PDF
1
* The thermal block units are sold separately as part numbers G8810A (96-well block)
and G8820A (384-well block).
**The USB external drive is provided for transferring files to and from the cycler as needed. We
recommend using this drive for all file transfers on the cycler.
Safety Precautions
Electrical
Standard electrical safety precautions should be applied, including the
following:
• Always put the instrument in a location where, if needed, the power
supply can be immediately disconnected.
• Proper voltage must be supplied before you turn on the instrument for
the first time.
• The device must be connected to a grounded socket.
• Do not touch any switches or outlets with wet hands.
• Turn off the instrument before you disconnect the power cord.
10
Agilent SureCycler 8800 Setup and User’s Guide
Before You Begin
Safety Precautions
1
• Unplug the instrument before you clean any major liquid spills and
before you service any of the electrical or internal components or swap
out the thermal block.
• Do not operate the instrument from a power outlet that has no ground
connection.
• Do not service the electrical components unless you are qualified to
do so.
Fluids and Reagents
• Fill reaction vessels outside the cycler so that no fluids penetrate the
instrument.
• Never cycle or incubate explosive, flammable and reactive substances in
the instrument.
• You must observe the relevant safety regulations when handling
pathogenic material, radioactive substances or other substances
hazardous to health.
• Do not submerge the instrument in any liquid.
Danger of Burns
• Do not touch the thermal block, inner side of heated lid and reaction
vessels. These areas quickly attain temperatures of greater than 50°C.
Keep the heated lid closed until temperatures of 30°C or lower are
reached.
• Do not use any materials (plates, sealings, foils, mats) which are not
sufficiently temperature-stable (up to 120°C).
Operating Environment
• The ventilation slots of the device must remain free to vent at all times.
Leave at least 10 cm of space around the instrument.
• Keep the ambient temperature between 20°C and 30°C with humidity
levels between 20% and 80% non-condensing.
• Do not operate the instrument in a hazardous or potentially explosive
environment.
Agilent SureCycler 8800 Setup and User’s Guide
11
1
Before You Begin
Safety Precautions
Equipment Ratings
• Pollution degree 2
• Installation category II
• Altitude 2000 m
• Humidity 20 to 80%, non-condensing
• Electrical supply 100-240 V, 50/60 Hz, 11 A
• Temperature 20°C to 30°C
• For Indoor Use Only
Electrostatic Discharge
The SureCycler 8800 is static sensitive. Electrostatic discharges greater
than 8000 volts may interfere with the normal operation of the USB ports
on the instrument. Handling precautions are required when working in
high static environments. Wear a grounded wrist strap and take other
antistatic precautions prior to making contact with the device in high
static environments. ESD STM5.1-1998 Class 3B.
Safety Symbols
The electrical/safety symbols described below may be displayed on the
instrument.
Power on
Power off
Caution
Caution, hot surfaces
12
Agilent SureCycler 8800 Setup and User’s Guide
Before You Begin
Indicator LED Light
1
Indicator LED Light
On the front of the instrument (top right corner) is a status indicator
LED. Table 2 summarizes the status codes for this LED light.
Table 2
Appearance of the status indicator LED
Appearance
Instrument Status
Off
The cycler is idle.
Blinking green
The cycler is running a program or incubation.
Solid green
The cycler is paused.
Blinking red
The cycler has detected an error. Check the display for an
error message containing further details.
Overview
The SureCycler 8800 is a thermal cycler designed to perform polymerase
chain reaction (PCR) and related methods for amplification of DNA
templates. The cycler can run even the most complex thermal cycling
techniques, including time and temperature increments, touchdown PCR
and temperature gradients. The software interface, operated through the
color touchscreen, features an intelligent Program Wizard that can create
PCR protocols automatically from primer and template information.
Agilent SureCycler 8800 Setup and User’s Guide
13
1
Before You Begin
SureCycler 8800 Technical Support
SureCycler 8800 Technical Support
Email
USA and Canada: [email protected]
Europe: [email protected]
Japan: [email protected]
All other countries: [email protected]
World Wide Web
www.genomics.agilent.com
Telephone
USA and Canada
(800) 227-9770
Europe
Austria
01 25125 6800
Benelux
02 404 92 22
Denmark
45 70 13 00 30
Finland
010 802 220
France
0810 446 446
Germany
0800 603 1000
Italy
800 012575
Netherlands
020 547 2600
Spain
901 11 68 90
Sweden
08 506 4 8960
Switzerland
0848 8035 60
UK/Ireland
0845 712 5292
Japan
0120-477-111
All other countries
Go to www.agilent.com/genomics/contactus.
14
Agilent SureCycler 8800 Setup and User’s Guide
SureCycler 8800
Setup and User’s Guide
2
Installation
Installation 16
Step 1. Select a location for the instrument 16
Step 2. Unpack the cycler 16
Step 3. Install the thermal block on the instrument base 16
Step 4. Connect the instrument to a power supply 17
Step 5. Connect optional devices 17
Step 6. Turn on the instrument 17
Step 7. Connect the cycler to a network (optional) 17
Thermal Block Exchange 18
To remove the thermal block 18
To install the thermal block 18
This chapter contains installation instructions for setting up the cycler
instrument.
Agilent Technologies
15
2
Installation
Installation
Installation
Step 1. Select a location for the instrument
• Locate a solid, flat clean surface for the instrument. Make sure:
• The instrument can stand completely stable.
• The rear air slots will not be covered.
• The unit always has at least 10 cm (approximately 4 inches) to the
next wall or neighboring instrument.
• The temperature (normal ambient) is between 20°C and 30°C with
humidity levels between 20% and 80% non-condensing.
• The atmosphere is not explosive.
Step 2. Unpack the cycler
1 Open the shipping containers and unpack the contents.
Detailed unpacking instructions are provided on the installation
poster (packed inside the same box as the instrument base).
Step 3. Install the thermal block on the instrument base
1 Using both hands, grasp the thermal block unit on the left and right
sides and place it on top of the instrument base.
2 Firmly press down on both sides of the thermal block unit until it locks
into place. Ensure both the left and right sides of the thermal block
unit are locked in place by pressing down on both sides.
The thermal block unit will only lock into place if it is placed on the
instrument base in the proper orientation.
For instructions on swapping out the thermal block, see “Thermal
Block Exchange” on page 18.
16
Agilent SureCycler 8800 Setup and User’s Guide
Installation
Step 4. Connect the instrument to a power supply
2
Step 4. Connect the instrument to a power supply
The instrument must be connected to a grounded AC outlet.
1 Plug the instrument’s power cord into the power connector at the rear
of the instrument.
2 Connect the other side of the cable to the outlet.
Step 5. Connect optional devices
If desired, you can connect an optional USB device (such as a keyboard
mouse or memory stick) to the instrument via the USB ports on the front
of the instrument.
1 Plug the USB cable of the device into either USB port on the
instrument.
Step 6. Turn on the instrument
1 Press the power button located near the bottom left corner on the front
of the instrument.
You can turn off the instrument any time. You do not need to close
the software before you turn off the instrument.
Step 7. Connect the cycler to a network (optional)
1 Plug one end of an ethernet cable into the ethernet port on the back of
the instrument.
2 Plug the other end of the cable into a network port.
Agilent SureCycler 8800 Setup and User’s Guide
17
2
Installation
Thermal Block Exchange
Thermal Block Exchange
Two interchangeable thermal block units are available with the SureCycler
8800: the 96-well block and the 384-well block.
To remove the thermal block
1 Power off the instrument and disconnect the power supply.
2 Lift the lid off the thermal block by pressing firmly on the gray button
on the top of the instrument.
3 Using both hands, grasp the thermal block unit on the left and right
sides while using your thumbs to press down on the buttons in the
upper left and right corners.
4 With the buttons firmly pressed, lift the thermal block unit up and off
the instrument base.
Use care when lifting the thermal block unit to avoid letting the lid
fall closed on your hands. Keeping the unit tilted slightly away from
you may help prevent the lid from falling closed.
To install the thermal block
1 Using both hands, grasp the thermal block unit on the left and right
sides and place it on top of the instrument base.
2 Firmly press down on both sides of the thermal block unit until it locks
into place. Ensure both the left and right sides of the thermal block
unit are locked in place by pressing down on both sides.
The thermal block unit will only lock into place if it is placed on the
instrument base in the proper orientation.
3 Reconnect the power supply when finished.
18
Agilent SureCycler 8800 Setup and User’s Guide
SureCycler 8800
Setup and User’s Guide
3
Getting Started
Sample Preparation and Loading 20
To prepare samples 20
To load samples 20
Agilent Plasticware and Consumables 21
Starting Up the Software 22
Touchscreen Operation of the Software 22
Home Screen 22
User Accounts 24
Access Levels 24
Logging In to User Accounts 24
Managing User Accounts 26
Network Settings and Remote Access 29
To view or edit the cycler’s IP address and name 29
To access the cycler from another cycler 30
To access the cycler from a computer 31
Cycler Settings 32
Hot Top Setting 32
Cycler name 33
Time and date 33
This chapter contains instructions to load samples, get started with the
software, set up user accounts and access instruments remotely via a
network.
Agilent Technologies
19
3
Getting Started
Sample Preparation and Loading
Sample Preparation and Loading
To prevent damage to the block and the heated lid, use only
temperature-stable sample tubes and plates. Unsuitable tubes and plates
can become damaged during cycling. See “Agilent Plasticware and
Consumables” on page 21 for a list of recommended plasticware.
WARN I NG
Danger of Burns: The thermal block, sample tubes and plates may reach
temperatures as high as 100°C. Keep hands away until temperature is 30°C or less.
To prepare samples
Keep the following guidelines in mind when preparing samples in reaction
plates and tubes:
• Place caps on tubes before loading samples into the thermal block.
• For best results, just before loading the samples into the thermal block,
spin them briefly in a centrifuge.
• If using sealing film to cover a reaction plate, use a compression mat
during cycling to ensure the film stays firmly adhered to the plate. See
“Agilent Plasticware and Consumables” on page 21 for ordering
information on a compression mat.
To load samples
The instrument can be loaded with individual PCR tubes or strip tubes or,
depending upon the thermal block installed, one 96-well PCR plate or one
384-well PCR plate.
1 Lift the lid of the thermal block by pressing firmly on the button on the
top of the instrument to unlatch the lid.
2 Put your plate or tubes on the block and check that they are correctly
positioned.
On the 96-well block, seat the tubes in the round holes on the thermal
block. On the 384-well block, seat the tubes in the larger round holes.
3 Close the lid until it latches into place.
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Agilent SureCycler 8800 Setup and User’s Guide
Getting Started
Agilent Plasticware and Consumables
3
Agilent Plasticware and Consumables
The following PCR plates, reaction tubes and sealing films are compatible
with the SureCycler 8800:
Agilent Part Number
Description
401333
96-well plate, 25-pack
410188
384-well plates, 50-pack
410186
Adhesive sealing films, 100-pack
410187
Compression mats, 10-pack
410082
Strip tubes, 200 l, 80 strips of 12
410086
Strip tube caps, 80 strips of 12
Agilent SureCycler 8800 Setup and User’s Guide
21
3
Getting Started
Starting Up the Software
Starting Up the Software
Touchscreen Operation of the Software
The color touchscreen allows you to operate the software by touching the
buttons on the screen. If a mouse has been connected to the instrument,
you may select buttons by clicking. When software functions require data
input from a keyboard, the software automatically displays a virtual
keyboard operated through the touchscreen. Data may also be entered
using a USB-connected keyboard.
Home Screen
Shortly after turning on the instrument, the touchscreen displays the
Home screen. This screen is the starting point for all the
software-controlled instrument operations as well as file and account
management. Each navigation button on the Home screen menu takes you
to a different functional area in the software.
The table below describes the function associated with each navigation
button on the Home screen:
Table 3
22
Home Screen Button Descriptions
Button
Description
New Program
Use this button to create a new PCR program.
Programs
Opens the file directory to browse stored PCR programs. Use this button to
edit or run programs.
PCR Wizard
Use this button to create a new PCR program using a guided Wizard.
Network Control
Use this button to connect to multiple instruments via a network.
Incubate
Starts a single temperature incubation for a predefined period or for an
indefinite hold.
Reports
Opens the report directory allowing you to browse for stored report files.
Agilent SureCycler 8800 Setup and User’s Guide
Getting Started
Home Screen
Table 3
3
Home Screen Button Descriptions (continued)
Button
Description
Login
Opens the Login screen.
Settings
Use this button to adjust cycler settings, upgrade software and manage
user accounts.
Agilent SureCycler 8800 Setup and User’s Guide
23
3
Getting Started
User Accounts
User Accounts
Access Levels
When the instrument is powered on, the Guest account is automatically
logged in to the software.
Three different access levels are available for user accounts: Administrator,
Registered user, and Guest user. Each level has specific user rights. Only
administrators are authorized to set up new users and to change and/or
assign user rights and passwords.
Table 4
Access levels
Access Level
Functions allowed
Guest
• Access rights to the Guest folder
• Creating, copying, editing and executing programs stored in the Guest
folder
User
• Access rights to the Guest and personal folders
• Creating, copying, editing and executing programs stored in the Guest
and personal folders
Administrator
•
•
•
•
Access rights to all Guest and user folders
Setup and maintenance of user accounts
Setup and maintenance of cyclers
Archiving/maintenance of all reports
Logging In to User Accounts
To log in as Guest
• No password is required to login as Guest. By default, the Guest
account is automatically logged into the system each time the
instrument is turned on.
24
Agilent SureCycler 8800 Setup and User’s Guide
Getting Started
Logging In to User Accounts
3
When logged in as Guest, you have restricted user rights and work from
the unprotected Guest folder, which can be accessed by all users. Before
you can operate the instrument as a registered user, an administrator has
to set up your new user account. Once you are a registered user, you have
a personal, encrypted file folder where you can manage your own PCR
program files.
To log in as Administrator
1 From the Home screen, click the Login button by either touching the
screen or clicking with a mouse.
The Login screen appears.
2 Select Administrator from the Username drop-down list and enter the
password. The default Administrator password is ADMIN. Use the Shift
key on the virtual keyboard to toggle between lowercase and uppercase
letters.
3 Press Login.
You are now logged in to the Administrator account.
CA U T I O N
Change the factory-set login password to prevent unauthorized access to the
instrument. See “To edit user accounts” on page 28 for instructions on changing
passwords.
To log in as a Registered User
1 From the Home screen, press Login.
The Login screen appears.
2 Select the user name from the Username drop-down list and type the
password.
3 Press Login.
This logs you into the system and gives you reading and writing rights
as specified for your user level.
An administrator can reset user passwords if needed. See “To edit user
accounts” on page 28.
Agilent SureCycler 8800 Setup and User’s Guide
25
3
Getting Started
Managing User Accounts
To change users
No logout process is required to change which user is logged in.
1 Press Login from the Home screen.
2 Select the new user from the Username drop-down menu.
3 If logging in with a registered user name or as Administrator, type the
password for that account.
If logging in as Guest, leave the Password text box blank.
4 Click or touch the Login button.
A confirmation message appears to verify the name of the user logged
in to the system.
Managing User Accounts
An administrator may use the Settings menu (accessible from Home
screen) to set up new users, edit user account information and delete user
accounts.
To add new user accounts
1 From the Home screen, press Settings.
2 Press User Management. (You must be logged in to an Administrator
account to access the User Management features.)
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Agilent SureCycler 8800 Setup and User’s Guide
Getting Started
Managing User Accounts
3
The User Management screen opens listing the available user names and
corresponding access levels.
3 Press Add to open the Add a New User screen.
4 In the User Name field, enter a user name.
5 In the Password and Confirm Password fields, enter a password for
the account.
6 Select the access level (User or Administrator) from the Access Level
drop-down list.
Selecting User will create a registered user account. Selecting
Administrator will give the user administrator rights.
7 Press OK to save the user account.
After successful setup, a confirmation message appears and the new user
name is added to the User Management list.
Agilent SureCycler 8800 Setup and User’s Guide
27
3
Getting Started
Managing User Accounts
To edit user accounts
1 From the Home screen, press Settings.
2 Press User Management. (You must be logged in to the Administrator
account to access the User Management features.)
The User Management screen opens listing the available user names and
corresponding user levels.
3 Select the user account to edit and press Edit to open the Edit User
screen.
4 From the Edit User screen, you can change the password and the
access level for the user account. (Note that the access level for the
Administrator and Guest accounts cannot be edited.)
5 Press OK to save the changes.
A confirmation message appears after successful update of the user
data.
To delete user accounts
The Administrator and Guest accounts cannot be deleted.
1 From the Home screen, press Settings.
2 Press User Management. (You must be logged in to the Administrator
account to access the User Management features.)
The User Management screen opens listing the available user names and
corresponding user levels.
3 Select the user name to remove.
4 Press Remove to remove the user name from the user list.
All user permissions for that account are deleted. All programs and
GLP reports associated with that user are deleted.
28
Agilent SureCycler 8800 Setup and User’s Guide
Getting Started
Network Settings and Remote Access
3
Network Settings and Remote Access
To view or edit the cycler’s IP address and name
1 From the Home screen, press Settings. You will be directed to the
Settings screen.
2 On the Settings screen, press LAN Settings. If the instrument is
connected to a network, the LAN Settings screen displays the IP
address, the subnet mask, the default gateway and the DHCP server.
You have the option of obtaining an IP address automatically or
assigning one manually.
• If you select the option Obtain an IP Address automatically, the
fields for network information are unavailable.
• If you select the option Use Manual Configuration, these fields are
editable and you can enter the desired information. If you do edit
the network information, press OK to save your changes.
Once logged in, you have control of the cycler from the PC using the
web-based SureCycler 8800 software. Note that some of the screens of
the web-based software differ slightly from the version of the software
installed on the instrument.
Agilent SureCycler 8800 Setup and User’s Guide
29
3
Getting Started
To access the cycler from another cycler
To access the cycler from another cycler
1 From the Home screen, press Network Control.
2 Press any of the six “Touch to Connect” squares on the screen.
A new screen will open.
3 Enter the IP address or cycler name of the instrument you want to
connect to into the IP Address/Cycler field.
4 Press Connect. You will be returned to the Network Control screen and
the network information and status of the connected instrument will be
displayed in the square.
5 Press the square for the connected instrument. You will be prompted to
login to the instrument.
6 Enter a valid User Name and Password to take over control of the
instrument remotely and press Log In. You are now operating the
software for the remotely connected instrument.
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Agilent SureCycler 8800 Setup and User’s Guide
Getting Started
To access the cycler from a computer
3
To access the cycler from a computer
1 From the computer, open an internet browser application (Microsoft®
Internet Explorer 6, 7 or 8, or Safari 5.0.2 is required).
2 In the URL field, enter the IP address for the cycler.
The SureCycler 8800 software will open on the computer.
3 Login to the connected cycler with an appropriate User Name and
Password.
To access the cycler from an iPhone
1 From the iPhone, open Safari (version 5.0.2 with iOS 4.3).
2 In the URL field, enter the IP address for the cycler.
The SureCycler 8800 software will open on the iPhone screen.
3 Login to the connected cycler with an appropriate User Name and
Password.
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3
Getting Started
Cycler Settings
Cycler Settings
Hot Top Setting
To enable or disable the use of the cycler’s heated lid (hot top):
1 From the Home screen, press Settings.
2 Press Hop Top Setting.
3 On the Hot Top Setting screen, make a selection for use of the hot top:
• Select Yes to use the hot top when the instrument is running.
• Select No to turn the hot top off.
4 Confirm your selection by pressing OK.
When the hot top is disabled, the lid does not heat up during PCR runs
and incubations. The dashboard (which is displayed on several of the
software screens) displays an icon to denote when the hot top has been
disabled. Below is an image of the dashboard that includes this icon.
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Agilent SureCycler 8800 Setup and User’s Guide
Getting Started
Cycler name
3
Cycler name
The name of the cycler can be changed by an administrator using the
following steps:
1 From the Home screen, press Settings.
2 Press System Setting.
3 Press the Change button located near the top of the screen. A new
screen will open prompting you to enter a new cycler name.
4 Confirm your changes by pressing OK.
Time and date
The time and date can be edited by an administrator using the following
steps:
1 From the Home screen, press Settings.
2 Press System Setting.
3 To change the date, select the correct date using the calendar under
System Date.
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3
Getting Started
Time and date
4 To change the time, press the up/down arrows to adjust the hour,
minutes or seconds listed under System Time.
5 Confirm your changes by pressing Apply.
34
Agilent SureCycler 8800 Setup and User’s Guide
SureCycler 8800
Setup and User’s Guide
4
PCR Programs
Creating New PCR Programs 36
To manually create programs 36
To create a PCR program using the PCR Wizard 39
Tips for Creating PCR Programs for the 384-well SureCycler 8800 42
Program Steps in the Commands List 43
Using a Preloaded PCR Program 51
Editing PCR Programs 53
Running a PCR Program 55
Viewing Reports 57
Troubleshooting 58
This chapter contains instructions to work with PCR programs,
incubations and reports. Troubleshooting suggestions are also provided.
Agilent Technologies
35
4
PCR Programs
Creating New PCR Programs
Creating New PCR Programs
New PCR programs can be created in one of two ways: by manually
creating a customized program step by step using a list of available
commands, or by using the Program Wizard to generate the program
automatically. The two methods are described in the following sections.
To manually create programs
Programs can be created manually from the programming screen. The
steps in the Command List are used to build the PCR program. The
Program Window lists the commands that have been added to the
program in the order of execution. Figure 1 shows an example of a
programming screen in which a Hot Start command has been added to
the program.
Figure 1
36
The programming screen of the SureCycler 8800 software.
Agilent SureCycler 8800 Setup and User’s Guide
PCR Programs
To manually create programs
4
Use the following steps to manually generate a new PCR program.
1 From the Home screen, press New Program. The programing screen
opens.
2 From the available program steps listed in the Command List on the
left side of the screen, determine the order of steps that will make up
the new PCR program.
See “Program Steps in the Commands List” on page 43.
3 Add the first step of the program from the Command List to the
Program Window using one of the following two approaches:
• Press the command then press the forward arrow button (shown
below).
• Double-press the command.
Both approaches open a new screen in which you can enter the
parameters for the selected step.
4 Enter the necessary settings for the newly added step as prompted.
5 Confirm your settings by pressing OK. You are returned to the
programming screen.
6 Repeat step 3 through step 5 for each program step to be added to the
program. You can change the order of commands in the Command
Window at any time using the up/down arrow buttons (shown below).
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PCR Programs
To manually create programs
7 To enter information about the PCR program (optional):
a Press the Program Information button (shown below). A new screen
will open.
b Press the keyboard icon (shown below) to access the touchscreen’s
virtual keyboard.
c Enter a description of the program. When finished, press OK to
return to the programming screen.
8 To save the file before running the PCR program.
a press the Save button (shown below). The Save Program screen will
open.
b Press the keyboard icon (shown below, left) to access the
touchscreen’s virtual keyboard and use the keyboard to type a name
into the Program Name field. Once the name has been entered, close
the virtual keyboard by pressing the icon again (shown below, right).
c Select the folder to which the program will be saved using the
directory at the bottom of the screen. You can press Create Folder if
a new folder needs to be created.
d Once the correct folder name is in the Save to User Folder field,
press Save to save the program.
e A dialog window will open indicating that the file was successfully
saved. Press OK in this window to return to the programming
screen.
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PCR Programs
To create a PCR program using the PCR Wizard
4
9 To start the program:
a On the programming screen, press Start.
b Enter the reaction volume (in microliters) when prompted and press
OK.
NOTE
For instructions on making edits to a PCR program, see “Editing PCR Programs” on page 53.
To create a PCR program using the PCR Wizard
As an alternative to manually creating a PCR program, you may use the
cycler’s PCR Wizard. The wizard walks you through a series of five steps
to automatically generate a new PCR program.
Starting the PCR Wizard
• Press the PCR Wizard button on the Home screen.
The 5 Steps of the PCR Wizard
1 Polymerase Selection
• Select a DNA polymerase from the list and press Next. The
polymerase options are:
•Paq5000 (hot start or standard version)
•PfuUltra II
•Herculase II
•PfuTurbo (hot start or standard version)
•Taq
The wizard algorithm determines the parameters of the program steps
based on guidelines specific to the chosen enzyme. (See the
documentation for the enzyme for cycling recommendations.) These
guidelines, however, are often broadly applicable and once the program
is generated and displayed in the programming screen, you are able to
edit the parameters of a step, or add/remove steps. See “Editing PCR
Programs” on page 53 for further instruction.
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PCR Programs
To create a PCR program using the PCR Wizard
2 Primer Information
• Enter primer information by one of two methods below. Press Next
when finished.
•Type in your primer sequences or browse to your sequence from a
connected USB external drive. We recommend that you use the USB
drive that came with your cycler.
•In the absence of sequence information, enter the melting
temperatures (Tm) of your primers.
The primer information is used to determine the annealing temperature
for the PCR program. The annealing temperature is set to 5°C below
the average Tm of the two primers. If you entered the base sequences
of the primers (rather than the Tm’s) the software calculates the Tm
for each primer based on the formula:
Tm = 69.3 + (0.41 × GC%) – (650/N)
where GC% is the percentage of GC content in the primer sequence
and N is the number of nucleotides in the primer sequence
3 Product Length
• Enter the expected product length and select the units as bp or kb.
Press Next when finished.
The Wizard uses the product length to set the elongation time of the
PCR program.
4 DNA Source Information
• Specify the source of template DNA from the list and then press
Next. The source options are:
•cDNA
•Genomic DNA
•Vector DNA
The DNA source information is used to determine the duration of the
hot start or initial denaturation step and the elongation step.
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Agilent SureCycler 8800 Setup and User’s Guide
PCR Programs
To create a PCR program using the PCR Wizard
4
5 Adjustments
• Select adjustments to the program.
•“GC” content larger than 70%? If the DNA template contains
greater than 70% GC base pairs, add this adjustment. The wizard
will adjust the PCR program to compensate for the higher melting
temperature of the template.
•Do you want a gradient annealing step? If you want the annealing
step of the PCR program to be a temperature gradient, add this
adjustment. The wizard will determine an appropriate gradient
range based on the melting temperatures of the primers.
Once the wizard has guided you through these five steps, it creates a PCR
program based on the provided information and displays it in the
programming screen with the steps of the program displayed in order in
the Program Window.
At this point, you may add, edit or delete steps in the same way as you
would for a manually created program (see “Editing PCR Programs” on
page 53).
To view or edit the parameters of any step, double-press the step in the
Program Window. A new screen will open showing the settings for that
command, such as temperature and duration. See the section “Program
Steps in the Commands List” on page 43 for more information on the
parameters for each command.
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PCR Programs
Tips for Creating PCR Programs for the 384-well SureCycler 8800
Tips for Creating PCR Programs for the 384-well SureCycler 8800
When performing PCR on the 384-well block, reducing the duration of the
denaturation step during cycling may improve product yield for certain
enzymes. Denaturation times as short as 3–5 seconds may be optimal in
some protocols.
If you use the PCR Wizard to design a PCR program, the program will
need to be edited after program creation to shorten the denaturation
temperature step. See “To edit program step parameters” on page 53 for
instructions on program editing.
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PCR Programs
Program Steps in the Commands List
4
Program Steps in the Commands List
The following program steps are available in the Command List for
creating customized PCR programs on the programming screen. The
description of each step includes its specific parameters that must be
defined when creating the program.
Hot Start
The Hot Start program step is an extended denaturation step that may be
added to the beginning of PCR programs that use polymerases with hot
start technology. The extended denaturation step activates the polymerase
by either removing a chemical modification agent or by denaturing a
polymerase-bound antibody. The Hot Start command can also be used as
the initial denaturation step in a PCR program when using polymerases
that do not have hot start capability.
When adding a Hot Start program step, you must specify the temperature
and time. During the program run, the next command will be executed
immediately after the hot start step.
Hot Start
Temperature:
Hold for
Enter the temperature for the Hot Start step.
Enter the duration of the step. Please consult the polymerase
manufacturer’s literature for recommendations on hot start conditions.
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PCR Programs
Cycle Wizard
Cycle Wizard
The Cycle Wizard command can be used to create the steps of the
program that are to be cycled multiple times. Using the Cycle Wizard to
create cycled steps replaces the need to manually add Start Cycle and End
Cycle commands. These steps will automatically be added to the program
by the wizard.
Denaturation
Step
Enter the denaturation temperature in °C (left field) and denaturation
time in hours : minutes : seconds (right field).
Annealing Step
Enter the annealing temperature in °C (left field) and annealing time in
hours : minutes : seconds (right field).
Elongation Step
If your PCR protocol uses 3-step cycling with separate annealing and
elongation steps (rather than a 2-step cycling protocol with a combined
annealing/elongation step), mark the check box labeled Use 3rd step.
Enter the elongation temperature in °C (left field) and elongation time in
hours : minutes : seconds (right field).
Cycles
44
Enter the number of cycles.
Agilent SureCycler 8800 Setup and User’s Guide
PCR Programs
Start Cycle
4
Start Cycle
This program step starts a PCR cycle. Put this step before a series of
steps that are to be repeated for several cycles.
Number of Cycles
Enter the number of times the series of steps should be cycled.
Cycle Name
(Optional)
If desired, enter a user-selected name for the cycle.
NOTE
To have a valid program, the steps of a cycle need to be contained between a Start Cycle
and an End Cycle command.
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PCR Programs
Temperature Step
Temperature Step
This program step holds the thermal block at a specified temperature for
a specified time. The user-defined parameters for this step also allow you
to set temperature or time increments (or decrements) with each cycle
and set the rate at which the thermal block reaches the set temperature.
Processing
Temperature
Hold for
46
Enter the temperature for the step (in °C).
Enter the duration of the step. Units are hours : minutes : seconds
Temperature
Ramp
Select the ramp rate for the instrument. The Fast ramp rate is the default
ramp rate for the instrument and is optimal in most PCR protocols. The
Standard ramp rate is provided as an option and could improve
amplification in some cases. Note that the Fast ramp rate improves
performance for some enzymes, including Agilent’s Herculase II DNA
Polymerase and PfuUltra II DNA Polymerase.
Step Type
Select the type of step from the drop-down. The options are denaturation,
annealing and elongation.
Agilent SureCycler 8800 Setup and User’s Guide
PCR Programs
End Cycle
NOTE
The fields in the Increments per Cycle section are used to set temperature and time
increments for the temperature step during cycling. The use of increments is an optional
feature. Leave these fields blank if you do not want to add increments.
Temperature
Increment
Enter the desired increase or decrease in the processing temperature with
each cycle. Press the +/– button to toggle between positive and negative
numbers. Negative numbers (e.g. –2°C) indicate an incremental decrease in
temperature with each cycle.
Time Increment:
4
Enter the desired increase in the Hold For time with each cycle. The units
for this field are seconds/cycle.
End Cycle
Insert this program step to terminate a PCR cycle. Put this step after a
series of steps that are to be repeated for several cycles.
NOTE
To have a valid program, the steps of a cycle need to be contained between a Start Cycle
and an End Cycle command.
Gradient Step
A Gradient Step is similar to a Temperature Step, but in a Gradient Step
the thermal block holds a temperature gradient (rather than a single
temperature across the entire block) for a specified length of time.
The Gradient Step is useful for optimizing the annealing temperature for
your particular primer/template system.
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PCR Programs
Gradient Step
During the run, the instrument generates a temperature gradient based on
the values entered into the Left Side Temperature Gradient and Right
Side Temperature Gradient fields. The difference between the minimum
and maximum temperatures must not exceed 30°C.
Left Side
Temperature
Gradient
Enter the desired temperature for the left side of the thermal block.
Right Side
Temperature
Gradient
Enter the desired temperature for the right side of the thermal block.
Hold for
Convert to
Temperature Step
(optional)
48
Enter the duration of the Gradient Step (units are minutes : seconds).
Once the optimal annealing temperature has been determined, the
Gradient Step can be easily converted to a standard Temperature Step by
marking this check box and specifying in the drop-down which
temperature column yielded the best amplification results. The Gradient
Step will then automatically be converted to a standard Temperature Step
in which the temperature of the thermal block is uniform.
Agilent SureCycler 8800 Setup and User’s Guide
PCR Programs
Touchdown
4
Touchdown
Touchdown PCR is an advanced PCR technique used to reduce nonspecific
primer/template binding. In the early cycles of touchdown PCR, the
annealing temperature is set relatively high to promote specific
amplification. Then, in later cycles, the annealing temperature is lowered
to permit more robust amplification.
The Touchdown program step is provided as a one-step command for easy
programming of touchdown PCR. You enter the maximum (starting)
annealing temperature and the minimum (ending) annealing temperature.
Then, during the run, the software executes the cycling protocol so that
the annealing temperature is lowered by an equal amount with each cycle.
Denaturation
Temperature and
Hold Time
Enter the denaturation temperature (in the Denaturation Temperature
field) and duration of denaturation (in the adjacent Hold For field) to be
used during touchdown cycling.
Max Annealing
Temperature
Enter the maximum annealing temperature. The first cycle of the PCR
program will use this annealing temperature.
Min Annealing
Temperature
Enter the minimum annealing temperature. The last cycle of the PCR
program will use this annealing temperature.
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PCR Programs
Storage/Pause Step
NOTE
Hold For
Elongation
Temperature and
Hold Time
Cycles
The difference between the maximum and minimum annealing temperatures is commonly
between 5°C and 10°C starting with a maximum temperature that is 2°C above the higher
primer melting temperature (Tm). For example, if the Tm of Primer 1 is 60°C and the Tm of
Primer 2 is 54°C, set the max temperature to 62°C and the min temperature to around 52°C.
Enter the duration of the annealing step.
Enter the elongation temperature (in the Elongation Temperature field)
and the duration of elongation (in the adjacent Hold For field) to be used
during touchdown cycling.
Enter the number of cycles for the Touchdown step.
Storage/Pause Step
The Storage/Pause program step cools the thermal block down to a
temperature between 4° and 12°C for a specified period of time or
indefinitely.
Hold Temperature
Hold for
Infinite
50
Enter the storage temperature in °C.
Select this radio button if you prefer to maintain the temperature for a set
duration of time. Enter the duration in the accompanying field.
Select this radio button to hold the specified store temperature for an
unlimited period of time.
Agilent SureCycler 8800 Setup and User’s Guide
PCR Programs
Using a Preloaded PCR Program
4
Using a Preloaded PCR Program
Several PCR programs come preloaded into the software for use on the
96-well SureCycler 8800. These programs were designed for performing
PCR with a variety of Agilent PCR enzymes and kits to amplify human
genomic DNA or vector DNA.
Each preloaded program is optimized to amplify targets of a particular
length with an annealing temperature that is suitable for some primers.
You may need to adjust the annealing temperature, the elongation time or
another cycling parameter used in the preloaded program to better suit
your particular target of interest. Any of the preloaded programs can be
saved under a new file name and then edited to optimize the program for
your primer/template system.
The available preloaded programs are listed in Table 5.
Table 5
Description of the preloaded PCR programs for the 96-well SureCycler 8800
PCR Program File Name
PCR Enzyme or Kit
Herculase II Fusion 1kb target.pcr
Annealing
Elongation
Herculase II Fusion DNA Polymerase 95°C for
20 seconds
55°C for
20 seconds
72°C for
30 seconds
Paq5000 HS MM 1kb target.pcr
Paq5000 Hotstart Master Mix
95°C for
20 seconds
55°C for
20 seconds
72°C for
30 seconds
PfuTurbo HS MM 1kb target.pcr
PfuTurbo Hotstart Master Mix
95°C for
30 seconds
55°C for
30 seconds
72°C for
1 minute
PfuUltra II HS MM 1kb target.pcr
PfuUltra II Hotstart Master Mix
95°C for
20 seconds
55°C for
20 seconds
72°C for
15 seconds
QuikChange II 4 kb target.pcr
QuikChange II Site-Directed
Mutagenesis Kit
95°C for
30 seconds
55°C for
1 minute
68°C for
4 minutes
QuikChange Lightning 4kb target.pcr
QuikChange Lightning Site-Directed
Mutagenesis Kit
95°C for
20 seconds
60°C for
10 seconds
68°C for
2 minutes
QuikChange Lighting Multi 4 kb
target.pcr
QuikChange Lightning Multi
Site-Directed Mutagenesis Kit
95°C for
20 seconds
55°C for
30 seconds
65°C for
2 minutes
QuikChange Multi 4kb target.pcr
QuikChange Multi Site-Directed
Mutagenesis Kit
95°C for
1 minute
55°C for
1 minute
65°C for
8 minutes
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Denaturation
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PCR Programs
To access the preloaded programs
To access the preloaded programs
1 Press the Programs button on the Home screen.
2 In the browser on the left side of the screen, navigate to the Guest\
Agilent folder.
The preloaded PCR programs files are saved in this folder.
3 Press the desired program to select it. Press Run to run the selected
program or Open to view the program parameters in the programming
screen.
To edit a preloaded program
1 Press the Programs button on the Home screen.
2 In the browser on the left side of the screen, navigate to the Guest\
Agilent folder.
3 Select the desired program and press Open.
The program will open in the programming screen.
4 In the programming screen, press Save to save the file under a new
name and/or directory location.
5 Edit the program as desired using the instructions provided in “Editing
PCR Programs” on page 53.
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PCR Programs
Editing PCR Programs
4
Editing PCR Programs
Programs that are created manually or by the Program Wizard can be
edited in the same way with the functions available in the programming
screen. Preloaded programs can also be edited once they are saved with a
new name (see “To edit a preloaded program” on page 52).
After you edit a PCR program, the program can be saved with the same
name or saved under a new file name. The USB drive on the front of the
instrument allow you to back up your saved PCR programs to a USB
external drive or transfer them to another SureCycler 8800 instrument. We
recommend that you use the USB drive that came with your cycler.
To edit program step parameters
1 Press the Programs button on the Home screen.
Depending on your user level (Guest or registered user), the specific
user directory or Guest directory is displayed listing all existing PCR
program files.
2 Select the program and press Open.
The program steps are displayed in the Program Window panel of the
programming screen.
3 To edit a particular step, double-press the program step.
The screen for that program step opens.
4 Edit the parameters as needed and press OK. You will be returned to
the programming screen.
5 Repeat step 3 and step 4 for each program step that requires editing.
6 In the programming screen, press Save. You can save your changes
under the same program name or assign a new file name.
7 You can press Start at this time to start the PCR program.
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PCR Programs
To copy a PCR step
To copy a PCR step
1 Go to the programming screen for the program.
2 In the Program Window, press the step to be copied to select it.
3 Press the copy button (shown below).
To add or delete a PCR step
• Go to the programming screen for the program, then:
• To add a new step, press a command on the Command List to select
it and then press the forward arrow button. You will be prompted to
enter the parameters for the new step. See “Program Steps in the
Commands List” on page 43 for details on parameters for a
particular command.
• To delete a step from the program, press the command in the
Program Window to select it and then press the delete button (shown
below).
• To save your changes to the program, press Save. You can save the
program under the existing name or give the file a new name.
To delete a PCR program
1 From the Home screen, press Programs.
Depending on your user level (Guest or registered user), the specific
user directory or Guest directory is displayed listing all existing PCR
program files.
2 Press the program to be deleted to select it.
3 Press Delete to remove the program from the folder.
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PCR Programs
Running a PCR Program
4
Running a PCR Program
To run a PCR program
From the Home screen:
1 Press Programs.
2 Select the program to run by pressing it.
3 Press Run Selected.
4 In the dialog box, enter the volume of the reactions in microliters and
press OK.
From the programming screen:
1 Press Start.
2 In the dialog box, enter the volume of the reactions in microliters and
press OK.
To stop or pause a program
From the Status screen:
• Press the Stop button (shown below) to abort the run.
• Press the Pause button (shown below) to pause the run. The Pause
button is only available when a program is actively running. When a
program is paused, this button becomes a Play button. Press Play to
restart the program.
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PCR Programs
Running an Incubation
Running an Incubation
The Incubate function is useful for setting up the instrument to run a
single-temperature incubation for either a specified period of time or
indefinitely.
To set up and run an incubation
1 From the Home screen, press Incubation.
2 In the Incubation Temperature field, enter the temperature of the
incubation.
3 Specify the duration of the incubation:
• If you want the instrument to hold the incubation temperature for a
specific length of time, select the Hold For option and enter the
desired duration time in hours : minutes : seconds.
• If you want the instrument to hold the incubation temperature
indefinitely, select the Infinite option.
4 Press OK to begin the incubation.
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PCR Programs
Viewing Reports
4
Viewing Reports
Every time a PCR program is started, a GLP report is created and saved
to user directory (for example, if the Guest account is logged in when the
PCR program is started, the report file is saved to the Guest directory).
Reports have the file extension glp.
To view reports
1 On the Home screen, press Reports.
This opens the Browse Reports screen listing the report files.
2 You can scroll through the list using the arrow buttons at the bottom of
the screen, or sort the reports by name or date. Press the Report Name
column heading to sort by name, or the Date column heading to sort by
date. You may also resize the columns by dragging on the divider on
the touchscreen.
3 Select the report to view and press Open. The report will open in a
new screen. When finished viewing, press the Back button to return to
the Browse Reports screen.
To delete a report
• Select the report on the Browse Reports screen and then press Delete.
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Troubleshooting
Troubleshooting
This section describes potential problems and recommended actions.
If PCR yield is low when running small reaction volumes with the
Standard ramp rate
✔ If you are using sealing films/mats, shorten the duration of the
denaturation step during cycling. Denaturation times as short as 3–5
seconds may be appropriate. Alternatively, switch to using capped
reaction tubes.
✔ Increase the reaction volume to 50 l.
✔ Try switching to the Fast ramp rate.
If no actual temperature is displayed during the run
The thermal block unit may not be correctly seated on the instrument.
✔ Check that the unit is installed correctly and reboot the cycler.
If the program does not start
The thermal block unit may not be correctly seated on the instrument.
✔ Check that the unit is installed correctly and reboot the cycler.
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If condensation is found in tubes during a run
4
If condensation is found in tubes during a run
Condensation will appear naturally at the end of a run and has no
deleterious effects.
✔ Spin the liquid back to the bottom of the tube and continue with the
post PCR steps.
The hot top is disabled.
✔ Enable the hot top using the instructions in “Hot Top Setting” on
page 32.
If the sealed microplate loses sample volume
✔ Check that the microplate/sealing system is of good quality.
✔ Ensure a compression mat is used during the PCR run to keep the
sealing film adhered to the plate.
If USB devices including memory stick and keyboard not
recognized
✔ Reboot the cycler.
✔ Use the USB external drive that came with your cycler.
If cycler does not turn on
✔ Check that the power is on at supply and the power cord is connected.
✔ Check fuses at rear of instrument above power inlet.
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PCR Programs
If screen is blank and unlit
If screen is blank and unlit
✔ Check that the power is on at supply and the power cord is connected.
✔ Check fuses at rear of instrument above power inlet.
If screen is lit but shows no information or has missing characters
✔ Reboot the cycler.
✔ If the problem persists, call Technical Support. See “SureCycler 8800
Technical Support” on page 14.
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SureCycler 8800
Setup and User’s Guide
5
Maintenance and Updates
Maintenance 62
Cleaning 62
Replacing a fuse 62
Software Updates 63
This chapter contains instructions for maintaining the instrument and
uploading software updates.
Agilent Technologies
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5
Maintenance and Updates
Maintenance
Maintenance
Cleaning
The SureCycler 8800 is designed to require a minimum amount of
maintenance by the user.
• Use water or isopropyl alcohol to clean the instrument.
• Do not let organic solvents or aggressive solutions come in contact with
the instrument.
• Do not let liquid enter the instrument.
• Turn off and disconnect the instrument from the power supply before
you clean.
Replacing a fuse
WARN I NG
Disconnect the power cord before you remove or install a fuse to avoid the
possibility of serious injury from electrical shock.
Fuse compartments are located on the rear of the instrument, above the
main power connection. Check the voltage rating label to verify that the
instrument is compatible with the AC voltage available at the installation
site. Check that fuses are rated as T 10A, 250V.
62
Agilent SureCycler 8800 Setup and User’s Guide
Maintenance and Updates
Software Updates
5
Software Updates
When a new version of the SureCycler 8800 software is released, you can
download the new version from the Agilent website and then upload it to
your SureCycler 8800 using the following instructions.
1 You will be notified by Agilent when a new version of the software is
available. This notification will include a web address where the
software files can be accessed. Save these files to a USB memory stick.
2 Login to one of the cycler’s user accounts that has Administrator access
(see “To log in as Administrator” on page 25 for instructions).
3 From the Home screen, press Settings and then press Software
Update.
4 Insert the USB memory stick into either of the USB ports on the front
of the cycler.
5 Press Browse to open a browser that allows you to navigate to the
folder containing the software files. Select the appropriate folder and
press OK to return to the Software Update screen.
6 Press Update. If the instrument does not detect any software files in
the selected folder, the Update button will be unavailable.
7 The cycler will begin downloading the files. When all files are
downloaded, the cycler will automatically reboot.
8 Once the reboot begins, remove the USB memory stick from the cycler.
9 When the reboot is complete, you may return to the Software Update
screen to confirm the new version of the software is running.
Agilent SureCycler 8800 Setup and User’s Guide
63
5
64
Maintenance and Updates
Software Updates
Agilent SureCycler 8800 Setup and User’s Guide
www.agilent.com
In This Book
This document describes how
to program and use the
Agilent SureCycler 8800.
Agilent Technologies, Inc. 2015
Version C3, June 2015
*G8800-90000*
G8800-90000
Agilent Technologies
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