OptiGrid User Guide

OptiGrid User Guide
USER GUIDE v1.4
This document discusses the use and architecture of the OptiGrid®
Structured Illumination System.
Introduction
The OptiGrid Structured Illumination System is an optical sectioning
device used for high-contrast fluorescence and ultra-sharp 3D imaging.
The OptiGrid Structured Illumination System consists of a paddle and a
controller box. The paddle component of the system contains an optical
grid that is controlled by a piezo drive for grid positioning and a DC motor
drive for grid focus along the optical axis.
Note: Manual versions will not have automated focus capability.
The OptiGrid works together with common microscope components and is
controlled by a host imaging application via a USB 2.0 communication
protocol. The OptiGrid Structured Illumination System is pictured below.
Figure 1: OptiGrid Structured Illumination System
Table of Contents
• Unpacking
• Waste of Electronic and Electrical Equipment
3
3
• Safety & Symbol Definition
• Service Requires Qioptiq Trained Personnel
• Special Handling Instructions
• Safety Standard Compliance
4
4
4
4
• What You Need to Get Started
• Standard OptiGrid Components
• Microscopes
• Computer and CCD/Frame
Grabber Requirements
5
5
6
• Installation
• Application Software
• OptiGrid Controller
• OptiGrid Paddle
• Cable Connections
7
7
7
7
8
6
• Operation
• Functional Overview
• Power (ON/OFF)
• System Status and Error Messages
• USB HID Class Communications Protocol
9
9
9
10
11
• Specifications
• Electrical
• Physical
12
12
13
• Global Regulatory Compliance & Certifications
• Safety
• Electromagnetic
• Environmental/ European Regulatory Compliance
15
15
17
19
• Customer Support and Services
20
• One Year Limited Warranty
21
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Unpacking
Remove the OptiGrid Structured Illumination System components from
the package.
The system is shipped with a paddle that has windows to protect the grid
from dirt while allowing light to pass through. Remove the paddle from its
plastic bag by grasping the metallic paddle handle. Never grasp the paddle
by touching the protective windows. Keep the windows clean and do not
scratch them.
Inspect all system components for any sign of damage. Contact your
OptiGrid reseller if the unit appears damaged in any way.
Waste of Electrical and Electronic Equipment
(WEEE)
This symbol on the product or on its packaging indicates that this product
must not be disposed of with your other household waste. Instead, it is
your responsibility to dispose of your waste equipment by handing it over
to a designated collection point for the recycling of waste electrical and
electronic equipment. The separate collection and recycling of your waste
equipment at the time of disposal will help to conserve natural resources
and ensure that it is recycled in a manner that protects human health and
the environment. For more information about where you can drop off your
waste equipment for recycling, please contact your local city office, your
household waste disposal service or the vendor where you purchased the
product.
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Safety / Symbol Definition
Service Requires Qioptiq Trained Personnel
DANGER
HAZARDOUS ELECTRICAL VOLTAGE
This symbol shown on the left is depicted on the back-plate of the
OptiGrid Controller. It indicates the presence of electric shock hazards.
The OptiGrid system components should only be opened by trained
personnel.
Special Handling Instructions
CAUTION: The OptiGrid Controller must only be used to operate the
OptiGrid paddle. Do NOT use it to operate other equipment.
The GRID CONTROL OUTPUT located at the rear of the OptiGrid
Controller produces voltages ranging from 40-150 V peak, DC to 1 KHz
when the unit is turned on. TAKE PRECAUTION. Do NOT touch the
output connector when operating the unit.
Safety Standards Compliance
The OptiGrid system meets the government safety regulations for
electrical equipment for measurement, control, and laboratory use in North
America and the European Union. In addition this product is certified for
compliance to the CB Scheme, a product safety certification that is
recognized in more than 30 participating countries.




European Union
- CE Marking: Standards IEC 61010-1 and EN 61010-1
- TUV Rheinland GS Mark
US: Occupational Safety and Health Administration (OSHA)
- cTUVus Marking: Standard UL61010
Canada: Standards Council of Canada (SCC)
- cTUVus Marking: Standard C22.2 No. 1010
International: Certification Body Scheme (CB Scheme)
- CB Certificate and Report with ALL national deviations
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What You Need to Get Started
To set up the OptiGrid Structured Illumination System, you will need the
following hardware and documentation listed and pictured below:
Standard OptiGrid Components
 OptiGrid Structured Illumination System Controller Box
 OptiGrid Structured Illumination System Paddle
Note: Different microscopes will require different paddles
 Grid Focus Cable (Motor Cable)
 Grid Control Cable (HI Voltage Output Cable)
 USB 2.0 Communication Cable
 Power Cord for the Controller Box
UK, Euro, or North American
 OptiGrid Structured Illumination System User Guide (not
shown)
Figure 2: OptiGrid Structured Illumination System Standard Components
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Microscopes
The OptiGrid Structured Illumination System integrates with a variety of
popular brand microscopes. Contact your OptiGrid reseller to determine if
a specific microscope can integrate with your OptiGrid System.
Olympus


IX 50, 51, 71, 81
o (Requires IX2-RFAL L-Shape Illuminator)
BX 41, 51, 61 Series
o (Requires BX-RFA/A Illuminator)
Nikon



E400, E600/FN & E80i/90i Series
o (Requires D-fl/E, 6 Position Universal EpiFluorescent Attachment or DIH-M (Digital Imaging
Head-M)
Epi-Fluorescent Attachment or D-DH Digital Imaging
Head M
TE2000/E/U/S Inverted
Zeiss


Axiovert 200/M
Axioplan 2 Imaging/MOT
Leica

DM 2000/2500/3000 (Requires Modified Illuminator)
Computer and CCD/Frame Grabber Requirements
Please contact your imaging software vendor for minimum computer
system recommendations and CCD/Frame Grabber support.
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Installation
This section explains and illustrates how to set up your OptiGrid
Structured Illumination System with your microscope and computer.
Complete the following steps to set up your OptiGrid Structured
Illumination System and configure it for use.
Application Software
Refer to your imaging software user’s manual for information
about how to control the OptiGrid Structured Illumination
System.
OptiGrid Controller
The controller physical dimensions are: 9.5”[24.1cm] x 8.0”[20.3cm] x
3.5”[8.9cm]. Rubber feet are attached to the bottom of the unit to prevent
skidding and allow it to be stacked on other desktop equipment that
requires circulation of air. The OptiGrid Controller requires at minimum
.078”[2mm] of clear air space above the unit.
CAUTION: The OptiGrid Controller must only be used to operate the
OptiGrid paddle. Do NOT use it to operate other equipment.
Optigrid Paddle
The paddle slides into the illuminator, usually replacing the field
diaphragm insert. The paddle has two click stops. One stop marks the
correct positioning in the illuminator’s beam-line. The other click stop
marks complete removal from the beam-line. It is not required to place the
paddle on the desktop when the OptiGrid is not being used.
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Cable Connections
1. Connect the USB 2.0 Cable between the USB 2.0 port on the
Controller (1) and a USB 2.0 port on the COMPUTER (1).
Note: The controller will also work on a non USB 2.0 port.
2. Connect the Grid Control Cable between the GRID CONTROL
OUTPUT port on the Controller (2) and the 4 pin receptacle on the
Paddle (2).
3. Connect the Grid Focus Cable between the GRID FOCUS port on the
Controller (3) and the 12 pin receptacle on the Paddle (3).
4. Connect the power cord to the AC IN power inlet module on the
Controller (4), and plug into local power (4).
Note:
For EMC compliance, operate this system with the cables provided
Figure 3: OptiGrid Structured Illumination System Cable Connections
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Operation
To following sections explain the functions of the OptiGrid Structured
Illumination System.
Functional Overview
The OptiGrid Paddle contains a one-dimensional optical grid mounted on
a piezo driven actuator and a focus motor attached to a Z-stage. The
OptiGrid Controller controls both the phase and focus position of the
optical grid via commands from the computer’s USB 2.0 interface.
When the OptiGrid Paddle is inserted into the light path of the microscope
illuminator, amplified voltage from the OptiGrid Controller is applied to
the piezo crystal to change its length. The piezo crystal provides highly
accurate repositioning of the grid pattern.
The grid pattern is systematically projected onto the specimen and is
moved perpendicularly to the grid lines across the sample. One structuredlight image consists of three split-second captures of the grid. The first
image is taken at any position of the grid; the grid is then moved linearly
by 1/3 of the grid period length to capture the second image, and another
1/3 to capture the third image. This typically occurs in less than one
second, generating one structured-light image or optical section.
Optically, the grid returns a strong signal wherever focus is sharp and a
weak signal where focus is soft. The patented OptiGrid algorithm
eliminates the weaker signals from above or below the primary image
plane as defined by the grid. The resulting image is free of any stray light
or soft focus data, and can be viewed live on your computer monitor in
near real time. The lateral resolving power of your selected microscope
objective is unaffected by the addition of OptiGrid. We recommend that
you choose an objective lens with a high degree of spherical aberration
correction and UV-transmission capability (Plan APO-Chromat is BEST).
Because OptiGrid utilizes the illumination source of the host wide-field
microscope, all fluorescence capabilities of that microscope are
maintained. When using fluorescence with the OptiGrid System, the
excitation and emission filters and dichloric mirrors should be optimized
to the specific fluorophore being used.
Power (ON/OFF)
The power ON/OFF control is located on the front panel of the OptiGrid
Controller.
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Factory System Status and Error Messages
System status messages are displayed on the OptiGrid (OG) Liquid
Crystal Display (LCD) located on the front panel of the OptiGrid
Controller. Tables 1 and 2 list the standard Status and Error Messages that
may be displayed on the OptiGrid LCD during the course of operation.
Your imaging software provider may choose to display additional status or
error messages to the OptiGrid LCD.
OptiGrid System Status Messages
Message
Description
Initializing
Displayed when OG is first turned on.
Wait until the message clears before operating the OG unit.
Default message when no other OG messages are displayed.
Default message when the host has set the OG for external DAC
control of the grid phase position.
Indicates that the grid focus position is at the HOME position
(minimum focal setting).
Indicates that the grid focus position is at the LIMIT position
(maximum focal setting).
Indicates that the host has set the OG for use with a “manual” (nonmotorized) paddle.
Indicates that the host has set the OG for use with a “motorized”
paddle (default).
Indicates that a connection between the host PC and the OG has
not been established.
Indicates the version of firmware that is installed on the OG.
OK
OK, External DAC
OK, Focus Home
OK, Focus Limit
OG Manual*
OG Motorized*
USB inactive
Version_XX*
*Note: Messages are only for OG firmware versions greater than Version 8.
OptiGrid System Error Messages
Message
FAULT, Focus Cable
FAULT, Focus Stall
FAULT, Phase Stall
FAULT, Piezo Cable
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Description
Indicates that the Grid Focus Cable is not connected.
Corrective Action: Power down and connect the Focus Cable.
Restart the system.
Indicates that the grid focusing mechanism is stalled.
Corrective Action: Call technical support.
Indicates that the grid phase mechanism is stalled.
Corrective Action: Call technical support.
Indicates that the Grid Control Cable is not connected.
Corrective Action: Power down and connect the Piezo Cable.
Restart the system.
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USB HID Class Communications Protocol
Developers
System developers and integrators should contact Qioptiq
Imaging Solutions for the latest Communications Protocol
for OptiGrid.
Qioptiq Imaging Solutions
78 Schuyler Baldwin Drive
Fairport, NY 14450
(585) 223-2370
[email protected]
End Users
Please contact your imaging software vendor for User Interface
instructions.
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Specifications
This section describes the physical characteristics of the OptiGrid
Structured Illumination System, along with its recommended operating
conditions.
Electrical
Power Requirement
Voltage…………………..…………………….............100-240~, 50-60Hz
Current…………………………………………………..0.3 A Twin Fused
Receptacle………………………………….1 A, 250V, Slow Blow, Fuses,
T/1A/L/250V
Focus Motor
Voltage…………………………………………………………..0-12 VDC
Current……………………………………………………………..500 mA
Phase Piezo
Voltage…………………………………………………………0-130 VDC
Current……………………………………………………………...200 µA
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Physical
OptiGrid Controller Dimensions
Length…………………..……………………..............................24.7 cm.
…………………………………………………………….(9.5 in.)
Width…………………………………………………………….20.3 cm.
…………………………………………………………….(8.0 in.)
Height…………………………………...........................................8.9 cm.
…………………………………………………………….(3.5 in.)
OptiGrid Paddle Approximate Dimensions
Note: Dimensions will vary with paddle model.
OptiGrid Controller I/O Connectors
GRID FOCUS….………………………………..12-pin Fischer metal DIN
GRID CONTROL…..……………………………...4-pin Lemo metal DIN
USB …………………………………............USB 2.0 Type B Receptacle
AC IN…………………….Standard AC Inlet with Twin-Fused Receptacle
DAC INPUT..………………………………………………………...BNC
OptiGrid Paddle I/O Connectors
GRID FOCUS………………………………..…12-pin Fischer metal DIN
GRID CONTROL.………………………………...4-pin Lemo metal DIN
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Operating Environment
Ambient Temperature………………………………………….0 to 40 ºC
Relative Humidity…………………………..10 to 80 %, non-condensing
Indoor Use Only
Pollution Degree 2
Storage Environment
Ambient Temperature……………………………………...…-20 to 70 ºC
Relative Humidity…………………………….. 5 to 95 %, non-condensing
High Speed USB 2.0
Transceiver……………………...Microchip, PIC18F4550 Microcontroller
Full Speed Transmission………………………………………......12 Mb/s
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Global Regulatory Compliance
and Certifications
Safety Compliance
Europe: CE Marking
This product meets the essential requirements of applicable European Directive
and standards, as amended for CE marking as follows:
Low-Voltage Directive
(safety)…………………………………………………………2006/95/EEC
Safety requirements for electrical equipment for measurement, control, and
laboratory use…………………….Standards IEC 61010-1 and EN 61010-1
Note: Refer to the Declaration of Conformity (DoC) for this product for any updates on regulatory
compliance information. To obtain a DoC for this product, contact Qioptiq Imaging Solutions.
Europe: TUV Rheinland GS Mark
The OptiGrid system has been evaluated, tested and approved for safety in
compliance with the TUV Rheinland GS Mark. The OptiGrid production line is
inspected annually to ensure that continuous production control is maintained.
The TUV Rheinland GS Mark is proof of product safety testing through an
independent third party test laboratory.
United States: cTUVus Mark
The OptiGrid system has been legally certified by a Nationally Recognized Test
Laboratory (NRTL) to comply with the consensus-based standards of safety. This
certification provides the assurance required by the Occupational Safety and
Health Administration (OSHA) that the product is safe for use in the U.S.
workplace.
Underwriters Laboratory Standard………………………………UL61010
Canada: cTUVus Mark
The OptiGrid system has been legally certified by TUV Rheinland, a test
laboratory accredited by the Standards Council of Canada (SCC) to comply with
Canadian national safety standards. This certification provides the assurance
required by the government that the product is safe for use in the Canada.
Standards Council of Canada Standard……………………C22.2 No. 1010
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International: Certification Body Scheme (CB Scheme)
The OptiGrid system has been certified by TUV Rheinland, a recognized National
Certification Body (NCB), to comply with the requirements of the CB Scheme for
ALL national deviations. This certification provides proof that the OptiGrid
product complies with international standards and is accepted by more than 30
international countries.
CB Scheme………………Certification and Report for ALL national deviations
This product is designed to meet the requirements of the following
standards of safety for electrical equipment for measurement, control, and
laboratory use:
 IEC 61010-1, EN 61010-1
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Electromagnetic Compatibility (EMC) Compliance
The OptiGrid system meets the requirements of the following standards of
electromagnetic compatibility for electrical equipment for measurement,
control, and laboratory use, Class B. Testing was performed by a third
party accredited by the American Association for Laboratory
Accreditation (A2LA).
Accreditation Certificate
Number……………………………………………………….1248-01
Note: For EMC compliance, operate this system with the cables
provided.
Europe: CE Marking
The OptiGrid system meets the essential requirements of the applicable
European Directive and standard, as amended for CE marking as follows:
Electromagnetic Compatibility
Directive…………………………………………………..89/336/EEC
EMC requirements for electrical equipment for measurement, control, and
laboratory use……………………………Standards EN 61326: 1997
+A1: 1998
+A2: 2001
+A3: 2003
Note: Refer to the Declaration of Conformity (DoC) for this product for any updates on
regulatory compliance information. To obtain a DoC for this product, contact Qioptiq
Imaging Solutions.
United States: Federal Communications Commission (FCC)
This product has been tested and found to comply with the limits for a
Class B digital device, pursuant to Part 15 of the FCC Rules. These limits
are designed to provide reasonable protection against harmful interference
in a residential or commercial installation. This product generates, uses
and can radiate radio frequency energy and, if not installed and used in
accordance with the instructions, may cause harmful interference to radio
communications. However, there is no guarantee that interference will not
occur in a particular installation.
If this product does cause harmful interference to radio or television
reception, which can be determined by turning the product off and on, the
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user is encouraged to try to correct the interference by one or more of the
following measures:
-- Reorient or relocate the receiving antenna.
-- Increase the separation between the product and receiver.
-- Connect the product into an outlet on a circuit different from that to
which the receiver is connected.
-- Consult the dealer or an experienced radio/TV technician for help.
FCC Regulation………………………CFR 47, Part 15, Subpart B
Canada: Standards Council of Canada (SCC)
This Class B digital apparatus complies with Canadian ICES-003.
Cet appareli numerique de la classe B est conforme a la norme NMB-003
du Canada.
If this product does cause harmful interference to radio or television
reception, which can be determined by turning the product off and on, the
user is encouraged to try to correct the interference by one or more of the
following measures:
-- Reorient or relocate the receiving antenna.
-- Increase the separation between the product and receiver.
-- Connect the product into an outlet on a circuit different from that to
which the receiver is connected.
-- Consult the dealer or an experienced radio/TV technician for help.
Canada
This Class B digital apparatus complies with Canadian ICES-003.
Cet appareil numérique de la classe B est conforme à la norme NMB-003
du Canada.
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Environmental Compliance
European Union
RoHS: Restriction of the Use of Certain Hazardous
Substances
The European Union RoHS directive (2002/95/EC) restricts the use of
lead, mercury, cadmium, hexavalent chromium, polybrominated biphenyls
(PBB) or poly brominated diphenyl ethers (PBDE) in new electrical and
electronic equipment put on the market in the European Union. The
RoHS directive applies to eight out of ten categories of electrical and
electronic equipment but excludes Category 9, Monitoring and Control
Instruments, which the OptiGrid® Enhanced Structured Illuminations
system falls. Qioptiq Imaging Solutions is actively working to release
RoHS-compliant products, even though the product is exempt from the
RoHS directive.
WEEE: European Union Waste Electrical and Electronic
Equipment Directives
The WEEE directives encourage the re-use, recycling, and recovery of
waste from electrical and electronic equipment. WEEE is intended to
improve the environmental performance of all operators involved in the
life cycle of electrical and electronic equipment, especially those dealing
with WEEE. The WEEE directive applies to ten categories of electrical
and electronic equipment, including Category 9, Monitoring and Control
Instruments, which the Optigrid Enhanced Structured Illuminations system
falls. Qioptiq Imaging Solutions is actively working to comply to the
WEEE directives as European legislation is passed.
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Customer Support and Services
Qioptiq Imaging Solution’s Web site provides access to technical
resources for the OptiGrid Structured Illumination System.
www.qioptiqimaging.com
Qioptiq’s Declaration of Conformity (DoC) is our self-declaration of
compliance with the European Union Directives. This self-declaration can
be obtained by contacting Qioptiq Imaging Solutions.
Qioptiq Imaging Solutions
78 Schuyler Baldwin Drive
Fairport, NY 14450
(585) 223-2370
[email protected]
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