GM-F6000 Thermal Cycler

GM-F6000 Thermal Cycler
GM-F6000 Thermal Cycler
User Manual
Document Version 1.5
GMbiolab Co., Ltd.
www.genemarkbio.com
Contents
About This Manual
A
B
4
Instrument Overview
1. Instrument Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5
2. Menu Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
6
Getting Started
1.Install Thermal Cycler. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.1 Site Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7
1.2 Unpack Thermal Cycler. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7
1.3 Power On and Power Off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7
1.4 Open and Close Lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
8
1.5 Block Module Exchange . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
9
2. Home Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
C
D
2
7
10
Manage Folder and Program
1. Manage Folder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
11
1.1 Program Library Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
11
1.2 Add Folder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
12
1.3 Rename Folder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
12
1.4 Delete Folder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
12
2. Manage Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
13
2.1 View Program Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
13
2.2 Rename Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
13
2.3 Copy Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
14
2.4 Delete Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
14
2.5 View Last Run Programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
14
Programming
1. Create New Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
15
1.1 Edit Program Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
15
1.2 Set Temperature Control Mode and Lid Temperature . . . . . . . . . . . . . . . . . .
16
1.3 Add Step . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
17
Constant Temperature Step . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
17
GM-F6000TM Thermal Cycler User Manual
E
F
1.4 Set Cycle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
19
1.5 Edit Step . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
20
1.6 Delete Step . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
20
1.7 Save Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
20
2. Edit Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
21
3. Program Wizard Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
21
Run Program
1. Load Samples into the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
23
2. Select Program to Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
23
3. View Run State and Control Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
24
3.1 View Run State . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
24
Run Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
24
View Run Timer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
25
View Bar Chart of Sample Block Temperature . . . . . . . . . . . . . . . . . . . . . . .
26
View Temperature Record Curve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
26
View Run Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
26
3.2 Control Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
26
Pause Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
26
Skip Run Step . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
26
Stop Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
26
Run Program Again . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
26
System Setting
1. System Setting Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
27
2. About . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
28
3. Incubation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
28
4. Gradient Calculator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
28
5. View Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
29
6. Tm Calculator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
29
7. Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
30
8. Configure Shortcut . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
30
9. Admin Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
31
9.1 Access Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
31
9.2 Thermal Cycler Name/IP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
32
9.3 Set Date/Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
33
9.4 Run-time Accuracy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
33
9.5 User Message . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
33
GM-F6000TM Thermal Cycler User Manual
3
About This Manual
Please read the manual carefully before using the Thermal Cycler!
Purpose of This
Manual
Writing
Conventions
This manual provides instructions on how to set up and use the Thermal Cycler.
We reserve the right to modify this manual at any time without prior notice.
This manual uses the following conventions:
• Bold text indicates user operation.
For example:
Touch Ok to accept changes and return to last screen.
• Note: Provides instructions that draw your attention or may be helpful.
For example:
Note:
When the instrument is set aside for a long time, disconnect the power cable
from the socket.
• IMPORTANT! Provides information that must be read carefully. Failure to
follow the information will possibly result in damage to the instrument or the
user.
For example:
IMPORTANT!
Turn the lid knob counterclockwise to loose the lid before opening the
lid to prevent damage to the lid.
IMPORTANT!
To prevent electric shock, please turn off the unit and disconnect the
power supply prior to cleaning.
Suggested
Operation
Gesture
Best Operating Angle
For optimum screen performance and
visibility, the angle shown at the left is
suggested for operation.
4
GM-F6000TM Thermal Cycler User Manual
A Instrument Overview
A
[ INSTRUMENT OVERVIEW ]
1. Instrument Components
Lid Knob
Lid
Lid Handle
Touchscreen
USB - A Port
Air Vent
Figure 1 Front View of Thermal Cycler
Air Vent
Power Switch
USB - B Port
Power Inlet
Ethernet Port
Figure 2 Rear View of Thermal Cycler
Table 1
Functions of Thermal Cycler Components
Component
Function
Lid
Heats the top surface of reaction vessels to prevent reaction sample evaporation and condensation.
Lid Knob
Regulates the height and force of lid.
Lid Handle
Opens and closes the lid.
USB - A Port
Connects to a USB flash drive.
Touch Screen
Views and controls the instrument.
Air Vents
Allows air to circulate into and out of the instrument.
Power Switch
Powers on (1) and powers off (0) the instrument.
Power Inlet
Connects the instrument to a socket via supplied power cable.
Ethernet Port
Controls multiple thermal cyclers with a computer.
USB - B Port
Connects the instrument to a computer.
GM-F6000TM Thermal Cycler User Manual
5
A Instrument Overview
2. Menu Overview
Table 2 and Figure 3 describe buttons and virtual keyboard common to many of the screens.
IMPORTANT!
Nails or the stylus pen supplied by the manufacturer is suggested to touch the touchscreen.
Table 2
Buttons in the screens of Thermal Cycler
Button
Function
Returns to the Home screen.
Closes current screen and returns to previous screen.
Moves between pages (i. e., Page Up and Page Down).
Scrolls left or right to view graph or curve.
Accepts present parameters setting and closes the screen.
Close the screen without changing any parameters.
Runs a selected program or incubation mode.
Figure 3 Virtual keyboard in the screens of Thermal Cycler
Texts are entered with the virtual keyboard:
To...
Touch...
Enter a number
A number button.
Enter a letter
A letter button.
Touch
/
to change between lowercase letters and
uppercase letters.
Join several words
Underscore character
Delete the last character in an
input field
Erase all characters
6
GM-F6000TM Thermal Cycler User Manual
B Getting Started
B [ GETTING STRATED ]
1. Installation
1.1 Site Requirements
The Thermal Cycler is intended for indoor use. Ensure that the installation site
meets conditions below.
Power supply
The Thermal Cycler has a universal, 85V-265V power supply and is supplied
with an appropriate plug.
Be sure that the instrument is connected to a reliable
power outlet that is rated to handle the load of the thermal cycler.
Environmental
Conditions
Table 1 Temperature and humidity conditions:
Condition
Acceptable range
Ambient temperature
10 to 30°C (50 to 86°F)
Relative humidity
20 to 80%, noncondensing
Place the Thermal Cycler on a level table or bench. Avoid placing the instrument
adjacent to heaters, cooling instruments, water sink or in direct sunlight. Keep
away from any equipment that vibrates, such as a refrigerator or centrifuge. The
recommended distance between the instrument and the back wall is 20 cm (7.9
in). Two thermal cyclers can be placed side by side.
1.2 Unpacking
1. Remove the packaging materials and keep in case of shipping damage to the
unit.
2. Inspect the instrument for shipping damage.
3. Included in the box should be:
• Thermal Cycler with block
• Appropriate power cord
• User Manual (this manual) including warranty certificate
• Performance Test Statement or pass card (if have)
IMPORTANT! If the Thermal Cycler is damaged or some items are
missing or damaged, contact our company or local distributor.
4. Move to the desired installation site.
1.3 Power On and Power Off
1. Connect the power cable to the power inlet in the rear of the instrument.
2. Connect the plug of the power cable to the socket. Make sure the power cable
has connected to both power inlet and socket firmly.
To power on: Turn the power switch to position ‘1’.
To power off: Turn the power switch to position ‘0’.
Note: When the Instrument is not used for an extended period of time,
disconnect the power cable from the wall socket.
GM-F6000TM Thermal Cycler User Manual
7
B Getting Started
1.4 Open and Close Lid
The lid of the Thermal Cycler can be adjusted to come into contact with and
apply pressure to the tops of the reaction vessels. Heating of the lid protects
samples from condensation.
IMPORTANT!
Temperature of the lid can be very high (105°C/221°F or higher). Never
touch the inner surface of the lid when the temperature is above 43°C.
To open the lid:
1. Turn the lid knob counterclockwise (see the ‘Loosen’ arrow symbol on the top
surface of the lid knob) to raise the pressure plate.
2. Grasp the lid handle so that the handle clamp is pulled toward to the lid
handle.
Lid Handle
Lid Knob
Handle Clamp
Chassis
3. Lift up the lid handle until the lid keeps opening vertically. You can then place
reaction vessels in the sample block or remove samples from the block.
To close the lid:
1. Place reaction vessels into the wells of the sample block.
2. Hold the lid handle and handle clamp, and then let the lid touch the chassis
until you hear ‘click …’ sound. The lid will be locked in place.
3. Turn the lid knob clockwise (see the ‘Tighten’ arrow symbol on the top
surface of the lid knob) to reduce the lid height until resistance us felt. Then
continue to turn the lid knob clockwise until you hear ‘click …’ sound. The
lid is now pressing on the reaction vessels.
1.5 Block Module Exchange
Block module exchange can be performed within seconds without needing any
tool.
IMPORTANT!
Please power off and disconnect the power cord from the socket before
replacement of the block.
8
GM-F6000TM Thermal Cycler User Manual
B Getting Started
To remove the block module
1. Open the lid
2. Put your hand on the left and right side of the block module and lift it up
vertically.
3. Store the block module in a clean, secure box.
To insert the block module
Put your hand on the left and right side of the block module, with the arrow on
block module pointing to another arrow on the chassis. Place the block into the
hollow cavity of the chassis and press tightly and evenly.
2. Home Screen
When the instrument powers on, the boot screen is displayed for several
seconds. The Home screen is displayed (see below).
a
Items in the Home screen:
b
a. Information Bar: lid temperature, block temperature (when the instrument is
idle or when running program which set temperature control mode as Block)
or sample temperature (when running program which set temperature control
mode as Sim-tube) and estimated remaining run-time.
b. Shows date, day and time.
GM-F6000TM Thermal Cycler User Manual
9
B Getting Started
Home Screen
Buttons
Button
Function
When login is enabled, touch this button to
logout the current user.
Opens Run screen.
LastRun Programs
Go to list of the last 10 run programs.
Go to list of folders, which contain all saved
Program Library
Program Wizard
programs.
Creates new program quickly.
Sets instrument parameters and shows other
System Setting
tools.
Configures
Empty Shortcut
Program Shortcut
10
shortcut
for
functions
or
programs.
Shows a selected program shortcut.
GM-F6000TM Thermal Cycler User Manual
C Manage Folder and Program
C [ MANAGE FOLDER & PROGRAM ]
1. Manage Folder
1.1 Program Library Screen
In the Home screen, touch Program Library.
The Program Library screen opens.
The table lists all available folders. Folder names include the number of
programs in parentheses. Touch the header of the first or second column of the
table to sort all folders. Touch
between pages.
(Page Up) and
(Page Down) to move
The Preset folder contains eleven preinstalled programs:
• 3Step_2000, 3Step_1000, 3Step_500: for amplifying less than 2kb, 1kb,
500bp nucleic acid templates using three-step PCR.
• 2Step_PCR: two-step PCR.
• Gradient: for optimizing annealing temperature of PCR reaction. This
program is only available with Gradient Thermal Cycler.
• Touchdown1 and Touchdown2: Touchdown PCR.
• Long_PCR: for amplifying of extra-long nucleic acid chain.
• RT_PCR: for synthesizing DNA from RNA by reverse transcriptase.
• Ligation_16°C: for connecting segmental DNA with vector DNA at 16°C.
• Enzyme_37°C: for ordinary enzyme reaction at 37°C.
Note: Programs in the Preset folder cannot be renamed or deleted. To
modify a program in the Preset folder, edit the program and then save to
another folder (see chapter D). Or copy the program to another folder (see
“2.3 Copy Program”), and then modify it.
The Public folder is a system folder and is used as the default folder for saving
programs.
Note: When login is enabled, all users have access to the Public folder.
GM-F6000TM Thermal Cycler User Manual
11
C Manage Folder and Program
When plugging a flash drive into the USB port, a USB icon
appears.
Touch this icon to open the USB folder. The USB folder contains programs that
are stored in the USB flash drive. Programs can be copied to and from the USB
flash drive.
Program Library
Screen Buttons
Button
Function
Shows programs of selected folder.
View
Creates a new folder.
New
Renames a selected folder.
Rename
Deletes a selected folder.
Delete
1.2 Add Folder
To add a folder:
1. In the Program Library screen, touch New.
2. In the New Folder screen, enter a folder name.
3. Touch Ok to accept changes and close the screen.
Note: The folder name is case insensitive.
1.3 Rename Folder
To rename a folder:
1. In the Program Library screen, select a folder and then touch Rename.
2. In the Rename Folder screen, Clear the old folder name, enter a new name,
and then touch Ok to accept changes.
Note:
The Preset folder, Public folder and USB folder cannot be renamed.
1.4 Delete Folder
To delete a folder:
1. In the Program Library screen, select a folder and then touch Delete. A
pop-up window appears.
2. Touch Yes in the displayed pop-up window to delete the folder.
Note:
The Preset folder, Public folder and USB folder cannot be deleted.
IMPORTANT!
When you delete a folder, all programs saved in the folder will be deleted
at the same time.
12
GM-F6000TM Thermal Cycler User Manual
C Manage Folder and Program
2. Manage Program
2.1 View Program Screen
1. In the Home screen, touch Program Library.
2. In the Program Library screen, select a folder and then double click it or
touch View to open the View Program screen. Touch the header of the first or
second column of the table to sort all programs.
View Program
Screen Buttons
Button
Function
Views and/or edits program
View/Edit
steps (see chapter D for
details).
Creates a new program (see chapter D for
New
Rename
Copy
Delete
details).
Renames a selected program.
Copies program between folders.
Deletes a selected program.
Runs a selected program (see chapter E for
Run
details).
2.2 Rename Program
To rename a program:
1. In the View Program screen, select a program and then touch Rename.
2. In the Rename Program screen, Clear the old program name, enter a new
name, and then touch Ok to accept changes.
Note: The program name is not case insensitive.
Programs in the Preset folder cannot be renamed.
GM-F6000TM Thermal Cycler User Manual
13
C Manage Folder and Program
2.3 Copy Program
To copy a program:
1. In the View Program screen, select a program and then touch Copy to open
the Copy Program screen.
2. In the Copy Program screen, select a Target Folder in the drop-down list box,
and then touch Copy to copy the program.
If you want to:
a. Rename the program before copy - Touch Clear to clear the old Program
Name, and then enter a new name.
b. Create a new target folder to copy the program to - Touch New Folder to
open the New Folder screen, enter a folder name, and then touch Ok to return
the Copy Program screen.
c. Touch Export to copy the program to a USB flash drive.
2.4 Delete Program
To delete a program:
1. In the View Program screen, select a program and then touch Delete. A
pop-up window appears.
2. Touch Yes in the displayed pop-up window to delete the program.
Note: Programs in the Preset folder cannot be deleted.
2.5 View Last Run Program
In the Home screen, touch Last Run Programs.
The Last Run Programs screen opens.
The Last Run Programs screen has the same appearance as the View Program
screen, but shows the recently run ten programs for quick viewing and repeat
runs. Touch Clear to clear the program list.
Note: The Rename and Copy buttons are not available in the Last Run
Programs screen.
14
GM-F6000TM Thermal Cycler User Manual
D Programming
D [ PROGRAMMING ]
1. Create New Program
1.1 Edit Program Screen
1. In the Program Library screen, select a folder and then double click it or
touch View to open the View Program screen.
2. In the View Program screen, touch New to open the Edit Program screen.
Note: To open the Edit Program screen more quickly, you can put the New
Program shortcut in the Home screen (see “Configure Shortcut” for details).
The Edit Program screen is used to create, view and edit program. After adding
several steps, the screen looks like below:
a
b
c
d
Items in the Edit Program screen:
a: Step number.
b: Temperature for a constant temperature step.
c: Step holding time (hour: min: sec).
d: The start step number and amounts of additional repeats for cycles.
GM-F6000TM Thermal Cycler User Manual
15
D Programming
Edit Program
Screen Buttons
Setting steps of a program are carried out via the buttons:
Button
Function
Sets temperature control mode and lid
Head
Temp
Gradient
Goto
Edit
Delete
temperature.
Adds a constant temperature step.
Adds a gradient temperature step.
Returns a former step and repeats.
Edits a step.
Deletes a step.
Saves
Save
the
created
program.
Saves another copy of the opened
Save as
Run
program.
Runs the program.
1.2 Set Temperature Control Mode and Lid Temperature
1. In the Edit Program screen, touch Head.
The Head screen opens.
2. Select temperature control mode and lid temperature control mode and then
touch Ok to accept changes.
Note:
The Sim-tube mode controls the process of heating the sample block to
include the sample volume in the equation (ie a larger sample volume
requires a longer time to reach temperature). Block mode does not consider
sample volume and simply heats the block to the set temperature.
16
GM-F6000TM Thermal Cycler User Manual
D Programming
Sim-tube is the preferred control method, especially when reaction steps are 30
seconds less. It is important to input sample volumes for the most accurate dwell
times. For lid temperature control, it is recommend to select a temperature five to
ten degrees Celsius above the highest temperature of the program steps (e.g., the
pre-denaturation step) to protect sample from evaporation and condensation.
Note:
The lid heating will automatically turn off when block temperature is below
30°C.
The lid will heat to the specified temperature before the actual
program starts running. Setting a lid temperature below ambient will cause the
program to stall.
1.3 Add Step
A PCR program comprises several steps. Each new step will be inserted after the
selected step while the initial step will be inserted without selecting a step.
The maximum amount of steps for a program is 30.
Constant
Temperature
Step
1. In the Edit Program screen, touch anywhere in the column for a step to select
it. When the step is selected, the background becomes blue.
2. Touch Temp and the Temp Step screen opens.
3. Edit Temperature and Time.
Note:
The allowable temperature range is from 0.0°C to 99.9°C, with 0.1°C interval.
The allowable time range is from 00:00:00 to 09:06:06 hour: min: sec.
• If you touch Pause, the program will automatically pause as soon as the desired
step temperature is reached and the instrument will beep once. Touching
Resume will continue the program running.
Pause can be used to add
reagents (ie hot start) or when adding or removing tubes during a step.
•
Touching Forever will cause the program to hold indefinitely after reaching
this step until it is stopped manually. Steps cannot be added after a forever
step.
GM-F6000TM Thermal Cycler User Manual
17
D Programming
4. You can also set options for a step.
• Increment Temp– adds temperature increments to step with each cycle.
To set the Increment Temp function:
Touch
to shift between “+” and “–” (to increase or decrease temperature
for each cycle), and then enter the number of degrees of temperature in the
textbox. The allowable temperature range is 0.1°C ~ 10.0°C.
Note:
Parameters cannot be set that would cause a step to fall outside of the
operating range of the instrument (ie below 0°C or above 99.9°C).
• Increment Time – Adds time increments to the holding time within the step
for each cycle.
To set the Increment Time function:
Touch
to shift between “+” and “–” (to increase or decrease time for each
cycle), and then enter the seconds in the textbox. The allowable time range is 1
sec ~ 120 sec.
Note:
Parameters cannot be set that would allow the dwell time to fall below zero.
• Slow Ramp – Adjust the heating or cooling ramp rate. The default ramping rate
is the fastest rate.
To set the Slow Ramp function:
Enter the value of ramp rate in the textbox. Input range is 0.1 ~ 6.0°C /sec.
Note:
The actual maximum ramp rate of heating and cooling varies with block
module type. Refer to the instrument specifications for the maximum value.
If a value larger than the block’s maximum rate is entered, the block’s
maximum rate will be used.
If you do not want to slow the running, leave
this option empty.
5. Touch Ok to accept changes and close the screen.
Gradient
Temperature
Step
18
1. In the Edit Program screen, select a step after which you want to add the
gradient temperature step.
2. Touch Gradient and the Gradient Step screen opens.
GM-F6000TM Thermal Cycler User Manual
D Programming
3. Touch Left and Right to enter temperature of the left (1st) and right (12th)
column of sample block.
The allowable temperature range: 30.0°C≤ Left< Right≤ 99.9°C
Difference between the Left and Right: ≤30°C.
4. Touch Calculate to display calculated temperature gradient from the first
column to the twelfth column.
5. Touch Time to edit the holding time. You can also set Pause or Increment
Time as you need. The allowable increment time range is 1 sec ~ 120 sec.
1.4 Set Cycle
1. In the Edit Program screen, select a step that you want to set as the last step of
a cycle.
2. Touch Goto and the Cycle Step screen opens.
3. From the Step drop-down list box, select the start step number of the cycle.
The steps between the last step and the start step will run repeatedly for the
specified cycle numbers.
4. Touch Cycles to add additional cycle numbers.
Note:
Allowable entered cycle numbers range: 1-100.
The actual cycle numbers will be ‘N+1’ where N stands for the entered cycle
numbers. To perform a PCR reaction with 30 cycles, then you should enter a
number of 29.
5. Touch Ok to accept changes and close the screen.
GM-F6000TM Thermal Cycler User Manual
19
D Programming
1.5 Edit Step
1. In the Edit Program screen, select a desired step.
2. Touch Edit to edit the step parameters.
3. Touch Ok to accept changes and close the screen.
1.6 Delete Step
1. In the Edit Program screen, touch a step to select it.
2. Touch Delete. A pop-up window appears.
3. Touch Yes in the displayed pop-up window to delete the step.
1.7 Save Program
1. In the Edit Program screen, touch Save or Save As to display the Save As
Program screen.
2. The current date and time are used as a default name. A new name can be
entered. The name is case insensitive and can be up to 30 characters.
Note:
Default program name:
last two digits of year
10 04 18 _ 13 49
month
day
hour
minute
3. From the Folder drop-down list box, select a folder to save the program to.
• To create a new folder to save the program, touch New Folder, and enter a
folder name of up to 30 characters.
4. Touch Run to both save and run the program.
Or touch Save to save but not run the program.
Or touch Export to save the program to a USB flash drive.
2. Edit Program
To edit a program:
1. In the Home screen, touch Program Library.
2. In the Program Library screen, select a folder and then double click it or touch
View.
20
GM-F6000TM Thermal Cycler User Manual
D Programming
3. In the View Program screen, select a program to be edited and then double
click it or touch View/Edit.
4. In the Edit Program screen, you can edit the program and then save and/or run
it following the instructions described above.
3. Program Wizard Function
The Program Wizard function provides a quick method to create a standard
three-step or two-step PCR program and perform running.
1. In the Home screen, touch Program Wizard.
The Program Wizard screen opens. There are two screens, touch Next and
Last to shift between them.
2. In the first screen, edit Temperature Control mode and Lid Temperature
Control mode. You can leave these parameters as default because the default
parameters is suitable for most PCR applications.
3. In the second screen, touch and edit parameters of each step. Keep parameters
of a step empty to exclude the specified step. You can touch Graph to view
the graphical representation of the program.
GM-F6000TM Thermal Cycler User Manual
21
D Programming
Note:
• Allowable temperature range: 0.0°C to 99.9°C.
• Allowable time range: 00:00 to 99:59 (min: sec).
• Allowable number of cycles: 0-101.
• The actual cycle numbers will be equal to the entered cycle numbers. So if
you would perform a PCR reaction with 30 cycles, then you should enter a
number of 30.
4. Touch Save to save program or touch Run and then select whether or not to
save the program before running according the displayed message in a pop-up
window.
22
GM-F6000TM Thermal Cycler User Manual
E Run Program
E [ RUN PROGRAM ]
1. Load Samples
1. Place PCR tubes or microplate in the sample wells of the sample block.
IMPORTANT!
Do not touch the surface of the sample block or lid surface because they
may be very hot (105°C/221°F or higher)!
Note:
•The cap of tubes or film on microplate must be sealed tightly to prevent
sample evaporation. Tubes or microplate should have heat resistance up to
about 120°C.
For best results, use thin walled tubes designed for PCR.
•The sample wells of the sample block should be kept clean.
•The height of tubes should be the same so that the lid can keep balance and
apply even pressure on each tube.
2. Close the lid and turn the lid knob clockwise (see the ‘Tighten’ arrow symbol
on the top surface of the lid knob) until you hear ‘click ...’ sound.
2. Select Program to Run
From the Home screen:
1. To select a last run program:
Touch LastRun Programs, select a program and then touch Run.
To select a program saved in a folder:
Touch Program Library, select the desired folder and then touch View to
open the View Program screen. Select a program from the list and touch Run.
After you touch Run, the Set Run Options screen opens.
GM-F6000TM Thermal Cycler User Manual
23
E Run Program
2. In the Set Run Options screen, edit Temperature Control and Lid Temperature
Control.
3. Touch StartRun to begin the run. The Run screen opens.
3. View Run State and Control Run
3.1 View Run State
You can view the status of the program running using the Run screen below.
Run Screen
b
a
c
d
The items displayed on the Run screen:
a: In the top bar are the colored status light (the colored status lights stays green
during running, changes to orange after touching Pause button, changes to red
when error arises and changes to white after stopping a run) and the run
status:
‘Lid’: lid temperature;
‘Block’ or ‘Sample’: block temperature (when the instrument is idle or
when temperature control mode is Block) or sample temperature (when
temperature control mode is Sim-tube);
‘Cycle’: current cycle numbers / total cycle numbers;
‘11:41:53’: Current Time.
b: Program/ Folder name (and Creator of program when login is enabled).
Note:
If you create a new program to run but did not save it, the program will be
given a default name ‘Admin’ and will be saved in a temporary folder ‘Temp’.
After run, the temporary program will not be recorded in the list of the Last
Run Programs screen.
c: Options:
Pause: preset pause;
X °C/Cyc: Increment or decrement temperature;
X S/Cyc: Increment or decrement time;
X °C/S: Ramp rate.
d: The recent run message.
24
GM-F6000TM Thermal Cycler User Manual
E Run Program
Buttons on the Run screen:
Button
Function
Pause/
Resume
Pauses and Resumes run.
Skips run step.
Skip
Stops run.
Stop
Views bar
Gradient
chart of sample block
temperature.
Views real-time temperature record
curve.
Curve
View Timer
Run Again
Views run time.
Runs program again.
Opens Edit Program screen to edit the
program.
Edit
Views run report.
Report
View Run Timer
1. In the Run screen, touch View Timer to view run time:
• Time Elapsed shows the time (hour: min) since the run has started.
• Time Remaining shows the estimated remaining time (hour: min).
2. Touch
to return to the Run screen.
Note:
The actual remaining time may slightly differ from the shown
remaining time.
View Bar Chart
of Sample Block
Temperature
1. In the Run screen, touch Gradient to view the temperature of each column of
sample block in real-time.
GM-F6000TM Thermal Cycler User Manual
25
E Run Program
Step Time: the countdown holding time of the current step after the step
temperature reached the setting value.
2. Touch
to return to the Run screen.
View
Temperature
Record Curve
1. In the Run screen, touch Curve to view the temperature record curve.
View Run
Report
1. In the Run screen, touch Report to open the Run Report screen.
2. Touch
to return to the Run screen.
The report contains records of run start time, run stop time, and manual
operations during running.
Touch Export to export the report in BMP format to a USB flash drive.
2. Touch
3.2 Control Run
Pause Run
to return to the Run screen.
In the Run screen, touch Pause and the system will beep for two times.
Note:
The temperature will continue to increase or decrease until it reaches the
setting temperature of a step.
When paused, the Pause button changes to Resume button, and you must
touch Resume to continue the program.
Skip Run Step
In the Run screen, touch Skip to skip the current running step and run the next
step.
Stop Run
In the Run screen, touch Stop and then touch Yes in the displayed pop-up
window.
Run Program
Again
26
In the Run screen, if the running program stopped, touch Run Again to run it
again.
GM-F6000TM Thermal Cycler User Manual
F System Setting
In the Home screen, touch System Setting.
1. System
Setting Screen
System Setting
Screen Buttons
The System Setting screen opens.
Button
Function
About
Shows product information
Performs a constant temperature incubation
Incubation
experiment.
Calculates gradient temperature of each
Gradient Calculator
View Report
Tm Calculator
Check
Configure Shortcut
Admin Options
column of the sample block.
Views stored run reports.
Calculates Tm value of a primer pair.
This function is reserved for service.
Configures functions or programs shortcuts.
Sets instrument parameters.
When a user other than “admin” login, the
Login Password
Admin Options icon will change to Login
Password’s icon. The user can edit their login
password using this function.
GM-F6000TM Thermal Cycler User Manual
27
F System Setting
2. About
To view product information about the Thermal Cycler:
1. In the Home screen, touch System Setting.
2. In the System Setting screen, touch About and the About screen appears.
3. When you finish, touch Ok to return to the System Setting screen.
3. Incubation
The Incubation function is used to perform a constant temperature incubation
experiment such as DNA ligation or RNA digestion.
1. In the Home screen, touch System Setting.
2. In the System Setting screen, touch Incubation.
3. In the Incubation screen, enter the required incubation temperature. If you
select Lid On, the lid will heat to reach 10°C above the incubation
temperature by default.
4. Touch StartRun to run the Incubation program and the Run screen opens.
5. In the Run screen, you can view block temperature (by touching Gradient),
view temperature record curve (by touching Curve) and run time (by
touching View Timer). You can stop running by touching Stop.
Note: The Pause, Skip, RunAgain, Edit, Report buttons are not available in
the Run screen under Incubation mode.
4. Gradient
Calculator
1. In the Home screen, touch System Setting.
2. In the System Setting screen, touch Gradient Calculator.
The Gradient Calculator screen opens.
3. In the Gradient Calculator screen, touch Left and Right to enter the
low-temperature and high-temperature of the left (1st) and right (12th)
column of the sample block.
The temperature range: 30.0°C≤ Left< Right≤ 99.9°C.
The difference between the Left and Right temperature: ≤30°C.
4. Touch Calculate to display calculated temperature gradient from the first
column to the twelfth column.
5. To leave, touch Exit to return to the System Setting screen.
28
GM-F6000TM Thermal Cycler User Manual
F System Setting
5. View Report
1. In the Home screen, touch System Setting.
2. In the System Setting screen, touch View Report to open the screen:
3. Select a report and double click it or touch Show to open the Run Report
screen.
4. After you finished, touch
to return to the View Report screen.
5. In the View Report screen, you can touch Clear to clear all reports.
6. After you finish, touch
6. Tm
Calculator
to return to the System Setting screen.
The Tm Calculator is used to calculate the Tm (melting temperature) and Ta
(annealing temperature) for a primer pair.
1. In the Home screen, touch System Setting.
2. In the System Setting screen, touch Tm Calculator to open the screen.
3. Enter the salt concentration and the primer concentration.
4. Enter each primer sequence and then touch Calculate to view the Tm value of
each primer, the average Tm value and the Ta value.
Note:
In order to develop a successful PCR reaction, it may be necessary to select a
different Ta value from the value on the screen.
5. After you finish, touch
GM-F6000TM Thermal Cycler User Manual
to return to the System Setting screen.
29
F System Setting
7. Check
This function is reserved for service.
8. Configure
Shortcut
Shortcuts appear on the Home screen and can access various functions or
programs. When login is enabled, each user can configure different shortcuts.
To configure shortcuts:
1. In the Home screen, touch:
• Any button labeled with ‘Shortcut’ or
• System Setting, then Configure Shortcut.
The Configure Shortcut screen opens.
2. In the Configure Shortcut screen, touch a check box next to a function on the
right of the screen to select a function as a shortcut.
3. If you want to select a program as a shortcut, touch Select Program.
4. In the Select Programs for Shortcut screen, select a folder from the Folder
drop-down list box and programs of the selected folder will appear. Touch the
header of the first or second column of the table to sort all programs. Touch a
program to select it and then touch Ok to return to the Configure Shortcut
screen. The program will appear in the left side list box.
5. When you finish, touch Ok to return to the Home screen.
The selected function or program will appear in the Home screen.
30
GM-F6000TM Thermal Cycler User Manual
F System Setting
Note:
You can select no more than six functions and/or programs as Shortcuts.
To deselect a shortcut on the Home screen, you need to touch System
Setting again, and then touch Configure Shortcut. In the Configure
Shortcut screen, unselect the check box for deleting a function shortcut,
or select the program from the program list and then touch Delete
Program for deleting a program shortcut.
9. Admin
Options
The Admin Options is used to control access mode, set thermal cycler name/IP
address, set date/time, set run-time accuracy, and manage user accounts. When
login is enabled, only admin can see the Admin Options button
.
To use Admin Options functions:
In the Home screen, touch System Setting, then Admin Options.
The Admin Options screen opens.
9.1 Access
Control
The Access Control is used to protect personal programs and folders and limit
ordinary users to modify instrument parameters.
To enable the Access Control:
When login is enabled, log in as “admin”.
1. In the Home screen, touch System Setting.
2. In the System Setting screen, touch Admin Options.
3. In the Admin Options screen, touch Access Control.
The Access Control screen opens:
GM-F6000TM Thermal Cycler User Manual
31
F System Setting
4. To enable the Access Control function, touch Require Login.
a. To allow anyone to create a new account in the Login screen, touch Allow
Create New Account by Anyone.
b. To allow an anonymous user to login, touch Enable Guest Account.
c. To allow login without password, touch Allow Login without Password.
5. Touch Ok, and restart the instrument by powering off the power switch.
After enabling the Access Control, you must login as a user on the Login
screen (see below) before you can use the instrument.
6. After you finished experiment, touch
9.2 Thermal
Cycler Name/IP
in the Home screen to log out.
To enable network control function, you need to set the IP address and a unique
Thermal Cycler Name for each instrument.
When login is enabled, log in as “admin”.
1. In the Home screen, touch System Setting.
2. In the System Setting screen, touch Admin Options.
3. In the Admin Options screen, touch Thermal Cycler Name/IP.
4. In the Thermal Cycler Name/IP screen, set a unique Thermal Cycler Name
and IP address. The name can be up to 30 characters.
5. When you finish, touch Ok to return to the Admin Options screen.
32
GM-F6000TM Thermal Cycler User Manual
F System Setting
9.3 Set
Date/Time
The date and time appear on the Home screen and the Run screen.
To set Date/Time (When login is enabled, log in as “admin”):
1. In the Home screen, touch System Setting.
2. In the System Setting screen, touch Admin Options.
3. In the Admin Options screen, touch Date/Time.
4. In the Date/Time screen, enter today’s date and current time.
5. Touch Ok to accept changes and return to the Admin Options screen.
9.4 Run-time
Accuracy
The Run-time Accuracy function is used to adjust coefficient of estimated
9.5 User
Message
The User Message function is used to manage user accounts.
remaining run time when the original estimated time is not accurate.
To manage user accounts (When login is enabled, log in as “admin”):
1. In the Home screen, touch System Setting.
2. In the System Setting screen, touch Admin Options.
3. In the Admin Options screen, touch User Message.
4. The User Message screen lists all accounts currently available.
Touch the table column header to sort all users.
• “admin”: control all Admin Options functions. The default password for
admin is admin.
• “Guest”: can create, rename, copy, modify, save, delete and run programs
that stored in Public Folder, Guest Folder and USB Folder.
• All other users: can create, rename and delete non system folders; create,
rename, copy, modify, save, delete and run programs; set his or her shortcuts;
modify his or her login password (in the System Setting screen after login).
In the User Message screen:
a. To create a new user account, touch Create to open the Create User screen.
Enter user name and password (case sensitive) of no more than 30 characters.
b. To edit user password, select a user and then double click it or touch Edit to
open the Edit Password screen. Enter new password and touch Ok.
c. To delete a user account, select a user and then touch Delete. Touch Yes in the
displayed pop-up window to delete the user.
GM-F6000TM Thermal Cycler User Manual
33
Appendix I Troubleshooting
[APPENDIX I] TROUBLESHOOTING
Problem
Cause
Possible Remedies
No display and no fan moving
No line voltage
Wait for power restore
Power cord unreliable
Check power cord and plug
sound
when power on
Unstable bench
Move to a stable bench
Abnormal vibrating noise
Uneven foot
Loose case screw
Move to a stable foot
Fasten the screw
Normal display but can’t heat
Heat pump fails or no current is
or cool
supplied
No display but fan is moving
LCD fails or LCD related circuit
when power on
fails
When power on, a message:
Lost power or power off the
“Warning! A power failure has
cycler when the last program is
occurred”
still running,(eg. Holding at 4°C)
Water accumulated in sample
wells
The cycler is running under 4°C
for a long time while the ambient
humidity is high.
USB flash drive is not
recognized
Press Run on display but no
action
Message: “Can’t find xx ( a
program on USB flash drive)”
and the screen is black when
power on
for at least 30 minutes.
Recommend
to
raise
the
storage
again.
is
or bad heat resistance.
evaporation
Running the block temperature to 93°C
drive is inserted
Tubes are of poor quality, too soft
of
last program to start a new one
Remove the USB flash drive and insert it
The USB flash drive
incompatible with the cycler
because
Touch OK to cancel the message. Stop the
Power lost after the USB flash
experiment
losing
Shut off and call service
temperature to 10°C.
Tubes are deformed after
Sample
Shut off and call service
Try another USB flash drive.
Use good quality PCR tubes.
Heat lid didn’t work or the
Turn on the heat lid and set the lid
temperature of lid is set too low.
temperature to be higher than 105°C.
Tubes, strips or microplate are of
poor quality
Microplate is not sealed tighten.
Sample volume is too small
Changes tubes, strips or microplate of
better quality
Ensure tight seal on the microplate.
Sample volume should be more than 10ul
Another program is running
A running program on USB flash
drive is restored but the USB
flash drive didn’t insert on cycler
Stop the last running program before
running a new one.
Touch OK to cancel this message and then
touch OK again to cancel the “Warning! A
power failure has occurred” message.
Touch Stop to exit
Adjust
Clock should be reset
Clock is abnormal
date
and
SettingAdmin
time
Options
via
System
Date/Time
screen
The battery in the cycler should
be changed
Warning message: “Instrument
Improper operation
failure! Error code: 01 to 05”
Instrument failure
GM-F6000TM Thermal Cycler User Manual
Shut off and call service
Shut off and contact the maunfacturer
34
GMbiolab Co., Ltd.
www.genemarkbio.com
E-mail: [email protected]
Was this manual useful for you? yes no
Thank you for your participation!

* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project

Download PDF

advertisement