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G-STORM
GS4M
Instruction Manual
G-Storm - 2011
Release Information
This document is protected by copyright. All Rights Reserved.
G-Storm.
Somerton Biotechnology Centre
Ricksey Lane
Catcombe
Somerton
Somerset. TA11 7JH
England
Phone: +44 (0) 7919 121510
Email: [email protected]
Web: www.gstormdirect.com
Manual Version:
1.0
Trademark Information
All product and brand names used in this document are trademarks or
registered trademarks of their respective holders.
Gradient options are not available in The USA, Japan and Germany
Copyright © 2011 G-Storm
II
G-Storm Thermal Cyclers
Contents
General Information ............................................................................................. 1
Policy Statement ............................................................................................... 1
Warranty Statement .......................................................................................... 1
1
Safety Warnings and Precautions .......................................................... 2
1.1 Before Operating the Instrument ................................................................ 2
2
1.1.1
Instrument Safety ....................................................................... 3
1.1.2
Symbols and Conventions ......................................................... 3
1.1.3
1.1.3.1
Potential Safety Hazards ........................................................... 4
ELECTRICAL ............................................................................. 4
1.1.3.2
EXPLOSIVE SUBSTANCES ..................................................... 4
1.1.3.3
PINCHING HAZARD .................................................................. 5
1.1.3.4
FLUIDS ...................................................................................... 5
1.1.3.5
DANGER OF BURNS ................................................................ 5
1.1.3.6
OPERATING ENVIRONMENT .................................................. 5
Installation ................................................................................................ 7
2.1 Requirements ............................................................................................. 7
2.1.1
Site ............................................................................................. 7
2.1.2
Electrical .................................................................................... 7
2.1.3
Thermal Blocks .......................................................................... 8
2.2 Unpacking the Thermal Cycler ................................................................... 9
2.2.1
Package Contents ...................................................................... 9
2.2.2
Initial Inspection ......................................................................... 9
2.2.3
Thermal Blocks ........................................................................ 10
2.3 Functional Overview ................................................................................. 11
2.4 Cycler Installation ..................................................................................... 13
2.4.1
Starting up the G-STORM ........................................................ 13
2.4.2
Logging in as Administrator ..................................................... 13
2.4.3
Cycler Validation Tests ............................................................ 14
2.4.4
Connecting Optional Devices ................................................... 14
2.5 AC Power Connection .............................................................................. 15
2.5.1
Operating Power Requirements ............................................... 15
G-Storm Thermal Cyclers
III
3
2.5.2
Fuses ....................................................................................... 15
2.5.3
Grounding Requirements ........................................................ 15
Software Operation................................................................................ 16
3.1 G-STORM and GSPC Software .............................................................. 16
3.2 Required Computer Skills ........................................................................ 16
3.3 Main Screen ............................................................................................ 17
3.3.1
Title Bar ................................................................................... 17
3.3.2
Status Bar ................................................................................ 18
3.3.3
Command Buttons ................................................................... 18
3.3.4
Additional functions ................................................................. 19
3.4 System Settings ....................................................................................... 20
4
3.4.1
Cycler Management ................................................................ 20
3.4.2
3.4.2.1
User Management ................................................................... 20
User Levels ............................................................................. 20
3.4.2.2
New Users ............................................................................... 21
3.4.2.3
Adding or Modifying User Accounts ........................................ 21
3.4.2.4
Deleting User Accounts ........................................................... 22
3.4.3
Cycler Settings ........................................................................ 22
Programming ......................................................................................... 23
4.1 Creating Programs................................................................................... 23
4.1.1
4.1.1.1
DND Programming .................................................................. 23
Program Steps ........................................................................ 25
4.1.2
Program Wizard ...................................................................... 30
4.2 General Programming Guidelines ........................................................... 31
4.3 Saving Programs ..................................................................................... 32
4.4 Editing Programs ..................................................................................... 32
4.4.1
Editing Program Parameters ................................................... 32
4.4.2
Deleting a Program Step ......................................................... 33
4.4.3
Converting a Gradient Step ..................................................... 33
4.5 Deleting Programs ................................................................................... 34
4.6 Incubate temperature .............................................................................. 34
5
IV
Run / Operation ...................................................................................... 35
G-Storm Thermal Cyclers
5.1 User Login ................................................................................................ 35
5.1.1
Guest Users ............................................................................. 35
5.1.2
Registered Users ..................................................................... 35
5.2 Changing Users ....................................................................................... 35
5.3 Making an Instrument Reservation .......................................................... 36
5.3.1
Cancelling a Reservation ......................................................... 36
5.4 Viewing Script Temperature Profiles Prior to Run .................................... 36
5.5 Loading the Cycler ................................................................................... 37
5.5.1
Loading Plates ......................................................................... 37
5.5.2
Loading Tubes ......................................................................... 37
5.6 Program Execution ................................................................................... 38
5.6.1
Starting a Run on a Single Cycler ............................................ 38
5.6.2
Starting a Run on Multiple Cyclers ........................................... 38
5.6.3
Viewing Current Program Step Information ............................. 40
5.6.4
Terminating a Run ................................................................... 40
5.7 Reports ..................................................................................................... 41
5.7.1
Viewing Reports ....................................................................... 41
5.8 Language Selection ................................................................................. 42
5.9 Switching Off the Instrument .................................................................... 42
6
USB Memory Stick Operation ............................................................... 43
6.1 Importing and Exporting Files .................................................................. 43
6.1.1
Exporting Program Files to a memory stick ............................. 43
6.1.2
Importing Program Files from a memory stick to the GS4 ....... 43
6.1.3
Uploading Primer Sequences from a memory stick ................. 44
6.2 Archiving Reports ..................................................................................... 44
7
Maintenance ........................................................................................... 46
7.1 Cleaning ................................................................................................... 46
8
Trouble-Shooting ................................................................................... 47
8.1 If a Power Failure Occurs ......................................................................... 47
8.2 Troubleshooting Information .................................................................... 48
9
Block Exchange ..................................................................................... 50
Removing the Block ................................................................................. 50
Gene Technologies Thermal Cycler
V
Inserting the Block ................................................................................... 50
Appendix ............................................................................................................ 51
Instrument Specifications ............................................................................... 51
Thermal Blocks ............................................................................................... 51
Heated Lid ............................................................................................... 51
User Interfaces ........................................................................................ 52
User & File Management ......................................................................... 52
Reports and Validation ............................................................................ 52
Barcode Option ........................................................................................ 53
Thermal Engine Characteristics............................................................... 53
Power & Dimensions ............................................................................... 53
Compatible Barcodes .............................................................................. 54
Compatible Plate Formats ....................................................................... 55
Order Information ........................................................................................... 57
Sales & Service .................................................Error! Bookmark not defined.
Index ... ..........................................................................................................59
VI
G-Storm Thermal Cyclers
General Information
Policy Statement
It is the policy of G-Storm to improve products as new techniques and
components become available.
G-Storm reserves the right to change
specifications at any time.
Warranty Statement
G-Storm guarantees that the thermal cycler you have received has been
thoroughly tested and meets its published specification.
This guarantee is valid for 24 months only if the product and functions have
been used according to the instruction manual. No liability is accepted for loss
or damage arising from the incorrect use of the thermal cycler. G-Storm's
liability is limited to the repair or replacement of the unit or refund of the
purchase price at G-Storm’s option. G-Storm is not liable for any
consequential damages.
G-Storm’s thermal cyclers are for research use only.
Read the Instruction Manual carefully before using the thermal cycler to
ensure that you obtain the best possible results from the machine.
G-Storm thermal cyclers should only be used by suitably qualified and trained
people. If the thermal cycler is not used as specified in this manual, the
protection provided by the equipment may be impaired.
G-Storm Thermal Cyclers
General Information
1
1 Safety Warnings and Precautions
1.1 Before Operating the Instrument
Ensure that anyone involved with the operation of the
instrument is instructed in both general safety practices for
laboratories and specific safety practices for the instrument.
Always place the instrument in a location where, if necessary,
the main power supply can be disconnected immediately.
2
Safety Warnings and Precautions
G-Storm Thermal Cyclers
1.1.1 Instrument Safety
Please read this section before operating the thermal cycler. Operators of this
instrument must be trained in both general laboratory safety practices and the
specific safety requirements of the thermal cycler. If the equipment is used in
a manner not specified by the manufacturer, the protection provided by the
equipment may be impaired.
All functions performed within the context of preparing, performing and
completing a run should be done with caution and care, and with general
respect both to the instrumentation and to associated chemicals, samples and
other devices.
1.1.2 Symbols and Conventions
The following chart is an illustrated glossary of the symbols that are used in
this manual.
CAUTION This symbol indicates a potential risk and alerts you to
proceed with caution
CAUTION This symbol indicates the presence of high voltage and
warns the user to proceed with caution
CAUTION This symbol indicates risks associated with hot surfaces
G-Storm Thermal Cyclers
Safety Warnings and Precautions
3
1.1.3 Potential Safety Hazards
1.1.3.1 ELECTRICAL
Standard electrical safety precautions should be applied.

Ensure that the proper voltage is supplied before turning the
instrument on for the first time.

The device must be connected to a grounded socket.

Do not touch any switches or outlets with wet hands.

Switch the instrument off before disconnecting the
AC power cord.

Unplug the instrument prior to cleaning up any major liquid
spills and prior to servicing any of the electrical or internal
components.
Only qualified personnel should perform electrical servicing.
1.1.3.2 EXPLOSIVE SUBSTANCES
Explosive, flammable and reactive substances should never be
cycled or incubated in any thermal cycler.
Do not operate the thermal cycler in a hazardous or potentially
explosive environment.
You must observe the relevant safety regulations when handling
pathogenic material, radioactive substances or other substances
hazardous to health.
4
Safety Warnings and Precautions
G-Storm Thermal Cyclers
1.1.3.3 PINCHING HAZARD
The closing lids present a potential pinching hazard. Do not place
hands or fingers over the cycler when the lids are being closed.
1.1.3.4 FLUIDS
Reaction vessels should be filled outside the cycler so that no fluids
penetrate the instrument.
Do not submerge the instrument in any liquid.
1.1.3.5 DANGER OF BURNS
The thermal blocks, inner side of heated lids and reaction vessels
quickly attain temperatures of greater than 50°C. Do not touch –
Risk of Burns! Keep the heated lid closed until temperatures of
30°C or lower are reached.
Do not use any materials (plates, sealings, foils, mats), which are not
sufficiently temperature-stable (up to 120 °C).
1.1.3.6 OPERATING ENVIRONMENT
Please ensure that the ventilation slots of the device remain free to
vent at all times. A space of at least 10cm should be left around the
thermal cycler
The ambient temperature should be between 10°C and 30°C, the
humidity between 0% and 95%.
G-Storm Thermal Cyclers
Safety Warnings and Precautions
5
WARNING
G-Storm trained service engineers must carry out all
repair work
Only original G-Storm replacement parts must be used.
Disconnect the instrument from the mains supply before
opening.
6
Safety Warnings and Precautions
G-Storm Thermal Cyclers
2 Installation
2.1
Requirements
The following steps must be followed for optimal and safe operation of the
thermal cycler:
2.1.1 Site

The thermal cycler should be placed on a rigid, flat, clean surface.
Make sure that the instrument is completely stable.

Adequate ventilation is important. Make sure there is
sufficient space so that the rear and side air slots are not
covered and to allow cooling air to circulate freely around the
instrument.

There should be no paper under the device as this may block
the ventilation path. The unit should always have at least
10cm distance to the next wall or neighbouring instrument.

G-Storm thermal cycler instruments were developed for operation
in laboratories in which there is normal ambient temperature and
no explosive atmosphere. The ambient temperature should be
between 10°C and 30°C the humidity between 0% and 95%.
2.1.2 Electrical

The thermal cycler must be connected to a grounded socket.
G-Storm Thermal Cyclers
Installation
7
2.1.3 Thermal Blocks

Reaction vessels should be filled outside the thermal blocks so that no
fluids penetrate the instrument.

To prevent damage to the blocks and the heated lids make sure you
use only recommended sample tubes.
Unsuitable tubes can be
damaged causing the escape of sample material. This may present a
serious health hazard, especially when working with infectious
materials.

Make sure plates are positioned correctly in the blocks to prevent
damage to the blocks and the lids.

Ensure that the heated lid is closed when heating tubes in a block,
otherwise tubes may burst due to high temperatures, causing the
escape of sample material.

The closing lid presents a potential pinching hazard. Do not place
hands or fingers over the cycler when the lids close otherwise hands
or fingers may be caught between the lid and housing.
8
Installation
G-Storm Thermal Cyclers
2.2
Unpacking the Thermal Cycler
2.2.1 Package Contents
The following parts are included in delivery:
G-STORM GS4M
Thermal Cycler
X
Power Cable 110 or
X
220V
Stylus (x3)
X
G-Storm Thermal
X
Cycler User Manual
on CD or a USB Stick
2.2.2 Initial Inspection
All parts of the instrument were thoroughly inspected and tested before the
unit was shipped, therefore the instrument should be in good operating order.
Carefully inspect the instrument and its accessories for any physical damage
sustained in transit.
If the instrument is received in a damaged condition, please file a claim with
the transport carrier immediately and contact G-Storm for advice.
G-Storm Thermal Cyclers
Installation
9
2.2.3 Thermal Blocks
The thermal blocks are shipped in separate packaging from the unit. They
should be removed from their packaging and located into position in the
server or satellite unit. Ensure that you push down the front and the rear
of the block so that the block is fully engaged in its correct position.
Figure 2-1: GS4M thermal cycler - thermal block installation
10
Installation
G-Storm Thermal Cyclers
2.3
Functional Overview
G-Storm thermal cyclers are automated instruments designed for carrying out
methods for amplification of DNA templates.
Power switch
USB Interfaces for
memory stick or
keyboard on left side
and rear
lids
Touch screen display
Figure 2-2: GS4M thermal cycler (profile view)
The G-STORM range is operated via its own internal PC and a universal
software application offering independent control of the thermal block.
G-Storm Thermal Cyclers
Installation
11
Figure 2-3: GS4M thermal cycler Main Screen/Home Page
12
Installation
G-Storm Thermal Cyclers
2.4 Cycler Installation
2.4.1 Starting up the G-STORM
Once you have set up the G-STORM as explained in section 2.1 and 2.2 you
can connect it to the mains.

Plug the AC power cord into the main power connector at the rear of
the instrument and connect the other end of the cable into a grounded
AC outlet.

Press the power switch on the side of the instrument.

The cooling fan powers up and the thermal cycler will go through a
short initialisation routine. Then, the Home Page appears providing
access to the thermal cycler’s navigation elements.
2.4.2 Logging in as Administrator
Only the Administrator is authorised to change cycler settings and run the
cycler validation tests:
1. Click on the Login button on the Home Page.
2. Select Administrator from the Username drop-down list and enter
the administrator password. The default administrator password is
admin.
3. Click Login.
4. You are now logged in as Administrator (visible in the top status
bar).
IMPORTANT:
It is recommended that the factory-set login
password be changed to prevent unauthorised access to the
instrument (see section 3.4.2.3)
G-Storm Thermal Cyclers
Installation
13
2.4.3 Cycler Validation Tests
Ensure a plate is in the block for this test:
1. Click Settings button
2. Click Browse cycler management
3. Click Cycler Tests
4. Highlight all cyclers in the top panel and click Engine Test.
When completed, click OK
Cycler Test procedures include specific instrument tests to ensure correct
installation and operation of the thermal cycler.
After the tests have been completed successfully, the instrument is installed,
operational and ready for use.
2.4.4 Connecting Optional Devices
Optional input devices such as keyboard, mouse and barcode reader can be
connected to the thermal cycler systems via a USB connection.

Plug the USB stick into the USB port of the input device.

Connect the other end of the USB to:
o
One of the USB ports of the GS4M (port at rear of instrument is
recommended), OR
o
a USB hub, if you wish to connect several input devices to a
GS4

When connecting several devices plug the cable connector of the USB
hub into the rear USB port of the GS4M.

For further configuration of the device consult the manufacturer’s
instruction manual.
14
Installation
G-Storm Thermal Cyclers
2.5
AC Power Connection
2.5.1 Operating Power Requirements
All G-Storm thermal cycler models have self-regulating power.
See
Instrument Specifications, Power & Dimensions for power specifications.
2.5.2 Fuses
WARNING:
Disconnect the AC power cord before
removing or installing a fuse to avoid the possibility of
serious injury from electrical shock!
Fuse compartments are located on the rear of the instrument, above the main
power connection. Check the voltage rating label to verify that the instrument
is compatible with the AC voltage available at the installation site.
2.5.3 Grounding Requirements
DANGER: please read carefully!
To protect operating personnel, the National Electrical Manufacturers;
Association (NEMA) recommends that the instrument be correctly grounded.
The instrument is equipped with a 3 conductor AC power cord that, when
connected to an appropriate AC power outlet, grounds the instrument. To
preserve this protection feature, do not operate the instrument from an AC
power outlet that has no ground connection.
G-Storm Thermal Cyclers
Installation
15
3 Software Operation
3.1
G-STORM and GSPC Software
G-STORM thermal cyclers are operated via their own internal PC running
under Windows and a universal software engine offering simple and intuitive
instrument control.
The unique Program WizardTM function enables stepwise protocol definition,
with no tedious calculations required. Drag-and-drop icon-based commands
make all steps quick, clear and intuitive throughout. Optimised conditions from
gradient protocols are converted to standard protocols with only a single point
and click.
The programming software can be installed additionally on any laptop or
desktop PC running WIN 2000 or XP. The memory stick supplied allows
quick and easy data and program exchange between PC and the thermal
cycler’s internal PC.
3.2
Required Computer Skills
Users should have basic computing knowledge including using the mouse,
selecting items, and drag and drop.
16
Software Operation
G-Storm Thermal Cyclers
3.3
Main Screen
Upon power up of the system, the screen first displays a start-up routine,
followed by the Main Screen/Home Page.
The Home Page is the main location for entering and displaying information.
Title Bar
Command
Buttons
Cycler Monitor
Status Bar
Figure 3-1: GS4M Main Screen with navigation elements
3.3.1 Title Bar
The Title Bar displays general information:
Displays contact details for service and application support
Cycler:
Displays the currently selected cycler
Program:
Displays the currently loaded program of the selected cycler
User:
Displays the current user
Displays the User Login screen
G-Storm Thermal Cyclers
Software Operation
17
3.3.2 Status Bar
The Status bar displays the following information:
Displays information on the current time
and temperature and defined target
temperature of a program step.
Opens the Reservation screen where
you can reserve the thermal cycler for
the next run. Upon successful
reservation, the name of the subsequent
user is displayed in the status bar.
3.3.3 Command Buttons
The Home Page Window comprises command buttons which provide quick
access to the functions that are available on the GS4M.
Run Program opens file list, enabling users to select program to
run
New Program opens the Compose/Edit a Program screen
View / Edit Program opens the file list to browse programs
Incubate opens the Incubate Temperature screen
Program Wizard opens the Program Wizard screen where you can
generate a program by entering biological information
18
Run/Operation
G-Storm Thermal Cyclers
Reports opens the report browser where you can search for stored
reports
Settings opens the System Settings screen where you can view &
edit cycler settings, manage users and run cycler tests
3.3.4 Additional functions
This logo serves as a Home button. Clicking on it
Home Page
from any screen returns user to the Home Page.
Displays status information of all cyclers, and
allows
user
to
control
multiple
cyclers
simultaneously.
The cycler buttons should also be used to select a
cycler prior to running a program. Double-clicking
these buttons displays the Status screen for each
cycler. If a program is running the Run Profile is
displayed.
Provides the operational status of each cycler
(flashes green when the instrument is running)
G-Storm Thermal Cyclers
Software Operation
19
3.4
System Settings
Specific system settings can be defined by clicking on the Settings button on
the Home Page screen (when logged in as an Administrator). Use the
Settings menu to add new thermal cycler devices or edit existing units, set
time and date, run cycler tests, or manage user accounts.
3.4.1 Cycler Management
Browsing this option allows an Administrator to edit cycler settings and
perform cycler validation tests.
3.4.2 User Management
Browsing this option allows an Administrator to manage cycler users.
3.4.2.1 User Levels
Three different user levels are available:

Administrator

Registered User

Guest User
Each level has specific user rights. Only administrators are authorized to set
up new users and to change and/or assign user rights and passwords.
By default, the thermal cycler starts with Guest user rights.
User Rights
Functions allowed
Guest User
 Access rights to the Guest folder
 Creating, copying, editing and executing programs
stored in the Guest folder
Registered User
20
Run/Operation
 Access rights to the Guest and personal user
folders
G-Storm Thermal Cyclers
 Creating, copying, editing and executing programs
stored in the Guest and personal folders
 Access to GLP reports denied
Administrator
 Access rights to all Guest and user folders
 Setup and maintenance of users
 Setup and maintenance of cyclers
 Archiving/maintenance of all reports
3.4.2.2 New Users
Only the administrator is authorised to set up new users and to change or
assign user rights and passwords.
As a first-time user you will be logged in as "Guest” user visible in the top
status bar. Guest users usually have only restricted user rights and work from
the unprotected Guest folder, which can be accessed by all users.
Before you can operate the thermal cycler as a regular user, your
administrator has to set you up as a new user. Once you are a registered
user, you have a personal, encrypted file folder where you can administer
your own program files.
3.4.2.3 Adding or Modifying User Accounts
NOTE: Only the administrator can set up new users or modify existing user
accounts.
To add a new user, click New User to open the Add a new User screen.
Assign a user name and password.
Select the required user level (Guest, User, Administrator) from the drop-down
list.
Now you can define the user’s script reading and writing privileges by
checking the appropriate boxes:
Edit/Rename Script
The user has the right to edit or rename a
program file
Delete Script
The user has the right to delete program files
Create Script
The user has the right to create program files
G-Storm Thermal Cyclers
Software Operation
21
Once you have entered all data, click on Create User to save the settings.
After successful setup, a confirmation message will be displayed and the new
user name will be added in the User Management list.
To modify an existing user, highlight the user name and click Edit User to
open the Edit a User screen.
Here you can modify user data such as name, user level, password, user
privileges and script directory protection.
Click OK to save the changes.
A confirmation message will be displayed after successful update of the user
data.
3.4.2.4 Deleting User Accounts
NOTE: Only the administrator can delete User accounts.
The User Management screen lists the available user names and
corresponding user levels.
Select the user name you wish to remove.
Click on Remove User to remove the user name from the user list. It removes
all user permissions that the user has and does not allow that user to access
any the thermal cycler device. It also deletes all scripts and GLP reports
associated with that user.
NOTE: The Remove User command also deletes all scripts and GLP reports
associated with that user. The Administrator user account cannot be deleted.
3.4.3 Cycler Settings
Browsing this option allows an Administrator to edit date and time settings and
select the language for the instrument. The Power Resume On/Off option
determines whether a program should continue or not after a power failure.
22
Run/Operation
G-Storm Thermal Cyclers
4 Programming
4.1
Creating Programs
G-STORM and GSPC software offers two innovative ways of composing
programs: Drop-and-Drag Programming (DND Programming) and the
Program Wizard.
4.1.1 DND Programming
Drag-and-Drop programming (DND programming) is an easy and intuitive
method for conveniently creating programs step-by-step.
The basic idea of DND programming is that you simply choose the required
command from a command list, drag it with the mouse into the program
window on the left and drop it into the desired program position. This opens a
window where you can define further command-specific settings such as time,
temperature, lid pressure, etc.
In this way you can create your program
command by command according to the planned programming sequence.
The command order can be changed easily any time just by dragging the
selected command to the desired position within the program window.
Once the program is started the cycler will process the commands from top to
bottom according to the defined programming sequence.
To generate a new program using DND, click
on the Home Page. This
will open the Compose / Edit a Program screen with its drag-and-drop
functions:
G-Storm Thermal Cyclers
Programming
23
Figure 4-1: Compose / Edit a program screen
The Compose / Edit a Program screen consists of the Command List, which
contains all available program steps, and the Program Window where you can
create or edit programs.
1. Select a command from the Command List, then drag and drop it into
the Program Window. This will open a new window for the selected
command.
2. Enter your settings in the relevant fields of the selected command.
3. Confirm your settings by clicking OK. This will return you to the
Compose / Edit a program window.
To add another command to your program, repeat steps 1–3 for each required
command.
Once you have finished creating your program, save it by clicking on Save As
and you will be prompted for a file name.
Name your file within Windows naming conventions.
Click on Profile, if you wish to view a graphical representation of the defined
temperature profile.
24
Run/Operation
G-Storm Thermal Cyclers
4.1.1.1 Program Steps
The following program steps are available in the Command List for creating
customised programs in the Program Window:
Barcode Input
Use this command to define barcode settings. When composing the program,
it is recommended to place the barcode command at the beginning. During
program run you will be asked to read the plate's barcode. You may do this
with a handheld USB barcode scanner or by manually entering the barcode. If
you use the barcode scanner, the barcode cannot be edited after reading.
The program will continue after you have placed the plate into the cycler and
pressed OK. The barcode will be recorded in the GLP and LabBook report
and displayed in the Info section of the Cycler screen.
Scan Using Barcode Entry: Barcode reading using the barcode scanner
Scan Using Keyboard Entry: Manual entry of the barcode via keyboard
Path for worklist: Specifies the path for the worklist.
Lid Temperature:
This defines the temperature of the heated lid. The
temperature may range from 112°C to 120°C. The
minimum increment is 1°C.
Lid is ON:
Select this option to activate lid heating. The heated
lid automatically switches off at temperatures below
25°C.
NOTE:
Heated Lid commands cannot be used within a cycle
G-Storm Thermal Cyclers
Programming
25
Automatic Hot Start
Automatic Hot Start: This step should be incorporated into a program when
using a hot start polymerase. Select the denaturation temperature and time.
When running the program, the next command will be executed immediately
after the denaturation time is elapsed.
Please also refer to the polymerase manufacturer’s recommendations
Manual Hot Start
Manual Hot Start: this step should be incorporated into a
program when using a standard polymerase. Select the
denaturation temperature and time. When running the
program, the instrument will notify you after the
denaturation time has elapsed. You will then be asked to
open the lid and add the enzyme. The instrument timer is
now on hold and will continue only after you have pressed
Temperature Step
Use this command to select the required temperature (in °C) and to define the
step duration.
Processing Temperature: Set the required temperature
NOTE: Temperature steps which are within a cycle must be 25°C or above
26
Run/Operation
G-Storm Thermal Cyclers
Hold for: Set the required time in hours, minutes and seconds. If the ‘hold at
this temperature’ is option is selected, the cycler will remain at the
temperature until the program is resumed. This allows the user to determine
when to continue to the next step.
Temperature Ramp: Rate of change of the sample temperature. Rates can be
from 0.1°C to 3°C per second. (Unit is defaulted to run at maximum ramp
rate).
Temperature Increment: Temperature increment and decrement per cycle.
Steps can be from 0.1 °C to 10 °C.
NOTE: For decrement use negative figures (e.g. -2)
Time Increment: Time increment per cycle in seconds.
Step Type: Indicates the selected step type for information purposes. The
supplied step type information will then be displayed in the cycler monitor.
Touchdown
Touchdown is an advanced technique used to reduce non-specific binding.
With the G-Storm thermal cycler, Touchdown is provided as a one-step
command for easy implementation in the programming sequence.
Denaturation Temperature: Defines denaturation temperature.
Maximum Annealing Temperature: Sets the maximum annealing temperature.
Minimum Annealing Temperature: Sets the minimum annealing temperature.
Hint: The temperature difference is commonly between 5°C and 10°C starting
with a temperature that is 2°C above the higher Primer melting temperature.
Example: if Tm Primer 1 is 60°C and Tm Primer 2 is 54°C, the resulting
Touchdown will be from 62°C to 52°C.
G-Storm Thermal Cyclers
Programming
27
Elongation Temperature: Sets the elongation temperature.
Cycles: Number of required cycles for the Touchdown section.
Duration: Duration of the temperature step in seconds.
Gradient Step
Use this command to define settings for the gradient function. The gradient
will span the blocks in the direction shown by the arrows below.
Enter the required minimum and maximum temperatures for the gradient and
duration.
Minimum Temperature Gradient: Enter the minimum temperature of the
gradient step. Temperature must not be below 30°C.
Maximum Temperature Gradient: Enter the maximum temperature of the
gradient step. Temperature must not exceed 80°C.
NOTE: The maximum / minimum gradient span is 30°C / 1°C.
Hold for: Duration of the Gradient step.
Convert Gradient Step to a standard temperature step: The Gradient step can
be converted easily to a temperature step after the annealing temperature
optimisation experiment. To do so, click the checkbox and select the
temperature column with the best result from your optimisation experiment.
28
Run/Operation
G-Storm Thermal Cyclers
The converted gradient step will then automatically use the temperature of this
best-performing column.
NOTE: Resulting temperature steps may be reconverted to
gradient steps any time by disabling the Conversion option
Gradient steps are not available in Japan, USA and Germany
Start Cycle
Starts a cycle.
Cycle Name: Enter the name of the cycle (optional).
Number of Cycles: Enter the required number of cycles.
End Cycle
Insert this command to terminate a cycle.
NOTE: All programs with a Start Cycle, must have an End
Cycle.
Store
Cools the thermal cycler down to a temperature between 4-12°C for a
specified period of time.
Store Temperature: Storage temperature in °C. The default temperature is
10°C
Hold for: Duration of the Store step.
Infinite: Holds the specified temperature for an unlimited period of time.
G-Storm Thermal Cyclers
Programming
29
Script Information
Use this command to record your comments about the program.
4.1.2 Program Wizard
The Program WizardTM is a unique feature that makes it easy for first-time
users to write their own protocol. The Program Wizard utilises biological data
to generate a program. Enter primer and template information e.g. Primer
sequence or melting temperature, template length and origin of DNA.
The Program Wizard is also an ideal tool for first shot reactions as it uses
proven calculations, e.g. for the Annealing Temperature.
To generate a new program click on Program Wizard on the Home Page.
1. Primer Information: Enter either primer sequences or melting
temperatures. Sequences can be uploaded from a .txt file on a
memory stick. Click Browse in the Sequence Primer One/Sequence
Primer Two section and browse for the required files in the Upload
Sequence from USB Memory Stick screen window. Select the
desired file and click Open to upload the file to the thermal cycler.
2. Product Length: Enter the length of your product either manually or
using the slide bar
3. DNA Source Information: Select from Bacterial, Eukaryotic or
Plasmid
4. Specials: Select any special requirements that your program may
require, e.g. type of enzyme, high AT, GC content.
5. After you have supplied all required data click on Generate Program.
The Compose / Edit a program screen window opens where you can
view and edit the program steps.
To edit a program step, double-click the respective command.
To view a graphical representation of the defined temperature profile, click
Profile.
30
Run/Operation
G-Storm Thermal Cyclers
4.2
General Programming Guidelines
To ensure a correct run, the following guidelines should be followed when
composing a new program:

A program must contain at least one Temperature Step.

Cycles cannot be nested. A started cycle must have an End Cycle
command before starting the next Start Cycle command.

Heated Lid and Barcode Input commands cannot be programmed
inside a cycle.

Only a single Script Information command is valid within a
program.
The following commands or command combinations are considered "stages":

Automatic Hot Start

Manual Hot Start

A string of up to ten single Temperature steps

Single Gradient steps

A complete cycle including Temperature or Gradient steps
within a start cycle / end cycle pair.

A Touchdown command

A Store command
When using these stages in a script, the following rules apply:

The maximum number of stages within a script must not exceed
10.

The maximum number of Temperature and Gradient commands
inside a cycle must not exceed 10.

The maximum number of cycles within a stage is 99.
G-Storm Thermal Cyclers
Programming
31
4.3
Saving Programs
To save a program script, click on Save As in the Compose / Edit a Program
window. Enter a program name and click Save to save the file to your user
directory. Users can create folders within their user directory to organise their
programs. After entering the program name, click Create Folder, enter a
folder name and click OK. Select ‘+’ beside the Scripts folder to show all
available folders, and click on the newly created folder. Click Save to save the
program script to this folder. A program can also be saved to an existing
folder by selecting it as described above.
4.4
Editing Programs
4.4.1 Editing Program Parameters
You can edit the parameters of existing programs and store the program with
the same name or rename the edited program.
To edit an existing program:

Click
on the Home Page
Depending on your user level (guest or registered user), the specific user
directory or guest directory is displayed listing all existing program files.
Select the program you wish to edit.

Click Open.
Opening the file automatically loads the program and the program
steps are displayed in the Program Window of the Compose / Edit
a Program screen.

Double-click the command you wish to edit. This will open the
window of the respective command.

32
Edit the parameters as needed.
Run/Operation
G-Storm Thermal Cyclers

Click OK to save your changes and to return to the Compose /
Edit a Program screen.

After you have modified all data, click Save in the Compose / Edit
a Program screen, if you wish to store your changes in the existing
program.

Click Save As, if you wish to rename the program. You will then be
prompted for a file name.
4.4.2 Deleting a Program Step
To remove a program step from the program just drag it from the Program
Window and drop it into the recycle bin
icon located below the Program
Window.
Save the program as described above.
4.4.3 Converting a Gradient Step
A gradient step can be converted to a standard temperature step with a few
mouse clicks.

After performing the gradient experiment, have a look at the gel image
and select the best-performing column.

Double-click the Gradient Step command.

Select the Convert Gradient Step to a standard temperature step
checkbox.

Select the appropriate column from the drop-down list in the
Conversion section.
The thermal cycler software will now convert the gradient step into a
temperature step and automatically select the right temperature in the
chosen column.
G-Storm Thermal Cyclers
Programming
33
NOTE: If it is required to repeat the gradient experiment, the converted
temperature step can be set back to a gradient command just by unchecking
the Conversion option
4.5
Deleting Programs
To delete an existing program:

Click
on the Home Page.
Depending on your user level (guest or registered user), the specific user
directory or guest directory is displayed listing all existing program files.
Select the program you wish to remove.
Click Delete. This will remove the program from the script folder.
4.6
Incubate temperature
To run the cycler for incubations or ligations utilise the Incubate temperature
function.

Click

Select the temperature that you require

Select the time you require
on the Home Page.
OR
34

Select infinity (Cycler will run until you Stop the program)

Select the heated lid, if required and the pressure setting.
Run/Operation
G-Storm Thermal Cyclers
5 Run / Operation
5.1
User Login
5.1.1 Guest Users
When powering up the instrument the default user level is Guest. If you wish
to continue to operate the cycler with Guest user rights, no special login
procedure is required. In this case you can access the unrestricted Guest
folder and operate the cycler as described in the relevant chapters.
5.1.2 Registered Users
Once the administrator has set you up as a registered user (see 3.4.2.3), you
can operate the thermal cycler using your personal data and file folders.
To log into the system as a registered user proceed as follows:

Click on Login – the Login screen opens.

Select your user name from the Username drop-down list and enter
your password.

Click on the Login button. This will log you into the system giving you
reading and writing rights as specified for your user level.

After successful login your user name is displayed in the top status
bar.
NOTE: In case a user forgets his or her password, the logged-on administrator
can assign him/her a new one.
5.2
Changing Users
In order to log into the system when another user is already logged in,
proceed as described in section Registered Users above.
G-Storm Thermal Cyclers
Run / Operation
35
5.3
Making an Instrument Reservation
The thermal cycler is equipped with a visual reservation function. The
reservation is not blocking or restricting the instrument to any user. It only
indicates that the instrument is needed for a certain user after the current run.

Click on the RESERVED FOR field in the right corner of the bottom
status bar.

Select Reserve next run for Current User, if you are already logged
in.

o
If you are not logged in, select Reserve next run for User to
reserve the subsequent run.
o
Select your user name from the Username drop-down list and
enter your password.
Click on Reserve to confirm the reservation.
5.3.1 Cancelling a Reservation
To cancel an existing reservation, check the Clear Reservation button.
To quit the reservation window without saving any changes, click Cancel.
5.4
Viewing Script Temperature Profiles Prior to Run
Before starting a run, you can view the temperature profile of a new or stored
program.
Click on Profile in the Compose / Edit a program screen window.
An information screen is displayed detailing all the parameters of the selected
program.
36
Run/Operation
G-Storm Thermal Cyclers
5.5
Loading the Cycler
Press the Open/Close button on the front of the instrument or click
on
the start screen to access the block.
WARNING:
Danger of Burns! The thermal block, test tubes and
plates may reach temperatures as high as 100°C! Before
loading or unloading the cycler, keep hands away until
temperature reaches 30°C or less
Pinching Hazard! Do not place hands or fingers in to the
cycler when the lid is being closed
5.5.1 Loading Plates
Depending upon the thermal block installed, the thermal cycler can be loaded
with one 96-well plate or one 384-well plate. Ensure the plates are used in
conjunction with a suitable seal.
Put your plate on the block and ensure that it is correctly positioned in the
provided space.
5.5.2 Loading Tubes
G-STORM thermal cyclers can be loaded with up to 96 tubes (0.2 ml) when
using the 96-well block.
G-Storm Thermal Cyclers
Run / Operation
37
5.6
Program Execution
5.6.1 Starting a Run on a Single Cycler
To start a run:

Select a cycler by clicking on the appropriate Cycler button and
click
on the Home Page.
Your personal file folder opens or, if you are logged in as a guest, the guest
folder opens displaying all available programs.
Select the program you wish to run.
Click Run selected on the right-hand side of the window, enter your sample
volume (2-150ul), and click OK to start the selected program.
5.6.2 Starting a Run on Multiple Cyclers
Select one of the cyclers you wish to use by clicking on the appropriate Cycler
button. Click
on the Home Page, and open the program which you
wish to run. Your program is now loaded on to this cycler. Click on the Cycler
Monitor button to bring up the Cycler Monitor screen (Figure 5-1). The profile
panes of all four cycles are displayed.
38
Run/Operation
G-Storm Thermal Cyclers
Figure 5-1: GS4M Cycler Monitor Screen
To copy your program onto other cyclers, click on the profile pane of the
cycler which has your program loaded, and click on the
button.
Select the additional cyclers on which you wish to run the program by clicking
on the appropriate profile panes, to highlight them and, click on the
button.
Your program is now loaded on to the cyclers.
appropriate cyclers are selected/highlighted, and click on the
Ensure that all
button.
Enter your sample volume (2-150ul), and click OK to start the selected
program.
Several cyclers can be controlled simultaneously from the Cycler Monitor
screen. Click on the profile panes to select/highlight the cyclers. The buttons
below the profiles:
can be used to control all of the selected cyclers.
G-Storm Thermal Cyclers
Run / Operation
39
5.6.3 Viewing Current Program Step Information
After a run has started, a graphical representation of the temperature
parameters is displayed on the Profile Screen. In addition, detailed program
and instrument information can be viewed using the Program or Info buttons
located in the right-upper corner of the information screen.
Click Program to display the program steps of the running protocol. The
executed step is highlighted.
Click Info to view detailed instrument information such as lid status, remaining
time and step time or GLP file name.
During a run, it is possible to return to the Home Page, and navigate the
command buttons, by clicking on the Home button. To return to the Profile
Screen, click on the relevant thermal cycler button (below Cycler Monitor) on
the Home Page.
You can view current program step information of all connected thermal
cyclers any time during the run by clicking on the Cycler Monitor button.
5.6.4 Terminating a Run
To stop a program manually before it completes, click the Stop button on the
Profile Screen, or on the Cycler Monitor screen.
Alternatively, use the Stop Program button on the Home Page (changes from
Run Program during a run).
The manual cancel of the program will be recorded in the GLP and lab book
reports
40
Run/Operation
G-Storm Thermal Cyclers
5.7
Reports
5.7.1 Viewing Reports
Depending on the assigned user level, you are allowed to view and archive
reports stored in the following file folders:
User rights
File folder
Action
Guest users
Guest folder
Viewing Lab Book and GLP reports
Registered users
Guest folder &
Personal file
Viewing Lab Book and GLP reports
folder
Administrator
All guest and
Viewing and archiving Lab Book and
user folders
GLP reports
To view reports proceed as follows:
Click Reports in the Home Page. This will open the Browse Generated
Reports window listing the generated reports.
Group the displayed reports by selecting one of the sorting criteria below:
Group by Date:
Sorts records in ascending order by date.
Group by Cycler:
Sorts records by cycler name.
Group by Program: Sorts records by program name.
For viewing GLP and/or LabBook reports select one of the following
options:
Show GLP:
Displays the generated GLP records (only for users
with administrator rights)
Show LabBooks:
41
Run/Operation
Displays the generated LabBooks
G-Storm Thermal Cyclers
5.8
Language Selection
G-Storm thermal cyclers can be operated in English (Default) and French.
To change the default language:

Login as an administrator

Click Settings on the Home Page

Click Browse on Cycler Settings

Select the language you require

Click OK
The unit will then remain in the selected language.
5.9
Switching Off the Instrument
The unit can be switched off at any time during operation using the power
button at the back of the instrument. There is no need to exit the thermal
cycler software prior to switching off the instrument.
42
Run/Operation
G-Storm Thermal Cyclers
6 USB Memory Stick Operation
6.1
Importing and Exporting Files
You can import Primer sequences and programs created on a different
thermal cycler or on a PC to the G-STORM thermal cycler and GSPC
software, or export programs and reports that have been created using a USB
memory stick.
6.1.1 Exporting Program Files to a memory stick

Make sure the USB memory stick is inserted in the USB slot on the
side of the instrument.

Click View & Edit Files to display your personal file folder. Files can
only be transferred individually.

Highlight the desired program by clicking on it once.

Click Archive Programs.

Click View USB to check that the file has been transferred.
NOTE: the lock button on the memory stick may prevent data access. Please
ensure the lock button is in the unlocked position.
6.1.2 Importing Program Files from a memory stick to the
GS4M

Make sure the USB memory stick is inserted in the USB slot on the
side of the instrument.

Click View & Edit Files to display your personal file folder.

Click View USB to access the USB files.

Highlight the desired program by clicking on it once.

Click Restore Programs.
G-Storm Thermal Cyclers
USB Memory Stick Operation
43
6.1.3 Uploading Primer Sequences from a memory stick
The Program Wizard TM function offers the option to upload primer sequences
stored in an external memory stick.

Click the Program Wizard button.

Click Browse in the Sequence Primer One/Sequence Primer Two
section and browse for the required files in the Upload Sequence
from USB Memory Stick screen window.

Select the desired file and click Open to upload the file to the thermal
cycler.
NOTE: Primer Sequence files must have *.txt file format.
6.2 Archiving Reports
GLP and LabBook reports cannot be deleted from the cycler.
An
Administrator is authorised to archive reports to a USB Stick, thus removing
them from the instrument.

Login as the Administrator

Insert the USB memory stick in the USB slot on the side of the
instrument.

Click the Reports button on the Home Page

Select the directory from which reports are to be archived. Click
Archive Reports (this button will only be visible when a USB stick is
detected)

A message will appear once the archiving is complete. Click OK.
To view and print archived reports, use the PC/demonstration version of GStorm software which can be downloaded from the Software page at
www.gstorm.co.uk/home.
Insert the USB stick containing archived reports
into the PC USB port.
Click on the Reports button and select Import
Reports.
44
All of the reports on the USB stick will be uploaded into the
Run/Operation
G-Storm Thermal Cyclers
software. To view and print a report, double-click on the desired report and
select Print.
G-Storm Thermal Cyclers
USB Memory Stick Operation
45
7 Maintenance
7.1
Cleaning
G-Storm thermal cyclers are designed to require a minimum amount of
maintenance by the user. They can be cleaned using water or a mild
laboratory-cleaning agent.
The instrument should not come into contact with organic solvents or
aggressive solutions. Ensure that no liquid enters the thermal cycler. For
safety reasons, the device must be switched off and disconnected from the
power supply prior to cleaning.
The electrical safety fuses are located above the main power switch and the
main power plug at the rear of the device. Before the fuses are replaced, the
device must be switched off and disconnected from the mains supply. Only
fuses with the correct voltage values may be used.
Only qualified service personnel should perform servicing of the thermal
cycler.
No warranty obligation is assumed by G-Storm in the event of damage caused
by unauthorised servicing.
46
Run/Operation
G-Storm Thermal Cyclers
8 Trouble-Shooting
8.1
If a Power Failure Occurs
All G-Storm thermal cyclers are equipped with an auto power fail restart
function that allows for power failures and safe continuation of a run after
resumption of power if set to do so in the cycler settings.
The autopower fail restart function restarts the script from the beginning of the
temperature step in which it failed.
The following table lists the three power failure scenarios and the actions the
instrument takes if the power is interrupted during operation.
Observation
Explanation / Remedy / Actions
The computer fails but the
thermal cycler unit still has
power
In this case the thermal engine will continue to
execute the script without any interruption.
The thermal cycler fails but
the computer still has power
The execution of the script will be affected and will
halt whilst the power is off. Once the power resumes,
restart the unit and the script will resume from the
beginning of the step it was executing when the power
failed.
Both the computer and the
thermal cycler unit lose
power
The execution of the script will be affected and will
halt whilst the power is off. Once the power resumes
the script will resume from the beginning of the step it
was executing when the power failed.
Corrective action:
Restart the application and the connection will be
restored.
47
Trouble-Shooting
Gene Technologies Thermal Cyclers
8.2
Troubleshooting Information
Refer to the following table for a description of potential problems and
recommended actions that you should take.
Observation
Explanation / Remedy / Actions
No actual temperature
displayed at the bottom of
the application
Block not correctly inserted in the machine.
Poor experimental yield
1. Run Thermal Engine OQ test (1) to check that
thermal engine operation is within specification.
Check that the block is inserted correctly and reboot
the machine.
2. If transferring protocol from a different make of
thermal cycler, use TAS (2) system to determine new
protocol parameters.
Program does not start
Block not correctly inserted in the machine.
Check that the block is inserted correctly and reboot
the machine.
48
Experiments work with tubes
but not with plates or vice
versa
Make sure correct lid pressure is applied.
Tubes are crushed
Applied pressure too high.
Condensation in tubes during
a run
Condensation will appear naturally at the end of a run
and has no deleterious effects. Simply spin or flick
the liquid back to the bottom of the tube and continue
with the post reaction steps. However, condensation
during a run will result in sub-optimal cycling. If this is
experienced try increasing the lid temperature.
Sealed microplate losing
sample volume
Poor quality microplate/sealing system or inadequate
pressure.
USB devices including
memory stock and barcode
scanner not recognized
Reboot machine.
Machine does not power up
and power light is not
illuminated
Check power is on at supply.
Blank Display lit but showing
no information
Reboot machine. If the problem persists there is an
internal connection problem: call Technical Support.
Abnormal block
heating/cooling performance
Run Thermal Engine OQ test (1) to check performance.
Run/Operation
Check fuses at rear of instrument above power inlet.
G-Storm Thermal Cyclers
(1) Please refer to the thermal cycler IQ/OQ Protocol document for
instructions on performing instrument qualification tests.
(2) The Temperature Acquisition System (TAS) can be purchased separately.
G-Storm Thermal Cyclers
Trouble-Shooting
49
9 Block Exchange
Block exchange can be performed within seconds. No tools are required for
this purpose.
CAUTION:
Turn power off and interrupt power line before changing
the block
Removing the Block
Remove the block by simply holding it with two hands on the left and right side
and lift it up.
Figure 9-1: Exchanging the block
Inserting the Block
Hold the block with two hands on the left and right side, with the block
connector at the back of the block, and place it into position in the thermal
cycler.
G-Storm Thermal Cyclers
Block Exchange
50
Appendix
Instrument Specifications
Thermal Blocks
Block Materials
Modular anodised aluminium blocks with 4
sensors
Traceability
NIST traceable temperature calibration
Blocks Available
-
96 well gradient block: For 96 well plates
or 96x 0.2 ml tubes
-
96 well Fast gradient block: For 96 well
plates, 96 x 0.2 ml tubes
-
384 well gradient block: For 384 well
plates
-
384 well Fast gradient block: For 384
well plates
-
In situ block for 4 slides
Heated Lid
Lid Temperature Range
112°C - 120°C, user selectable in 1°C
increments
Lid Pressure Range
Manual lid pressure setting. Compatible with
all vessels & seals
Ambient temperature
10°C to 30°C
Relative Humidity
0% to 95%
G-Storm Thermal Cyclers
Appendix
51
User Interfaces
Screen Type
System Input
8.4-inch TFT colour touch-screen,
stylus supplied.
Touchscreen, external keyboard
(Optional) & mouse (USB) (Optional),
barcode scanner (USB) (Optional)
Temperature Display
Real-time graphical display of actual &
set block temperatures
Communication Interfaces
1x USB front (personal memory stick,
keyboard, barcode reader, mouse,
USB hub)
1x USB rear
User & File Management
User Level Management
3 levels: Administrator, User with
administrator
selectable
restricted
rights, Guest
User File Protection
Yes, for programs and reports
File Organization
Windows® Explorer, user-defined
folders & subfolders
Program Storage
Minimum 99 (internal memory or USB
stick). Circa 500. Unlimited PC
program storage
Reports and Validation
Report Function
Encrypted GLP report, LabBook report
Validation
Internal auto validation prior to each
program start
Thermal engine validation function
52
Appendix
G-Storm Thermal Cyclers
Barcode Option
Networking
Barcode Option
PC controlled cycler network up to
15 units via USB hubs
Barcode documentation via
handheld barcode scanner
Thermal Engine Characteristics
Temperature Control
4°C - 99°C with simulated volume
dependent control algorithm
Sample Volume Range
5-150µl
Sample Accuracy
+/- 0.4°C (20-99°C), +/- 1°C (4-20°C)
Sample Homogeneity
+/- 0.4°C
Heating
Up to 3°C per second
Cooling
Up to 3°C per second
Sample Overshoot
< 1°C
Gradient Temperature Range
30°C - 80°C
Maximum/minimum Gradient Span
30°C / 1°C
Power & Dimensions
Electrical Power Supply
100V to 240V (frequency 48 to
62Hz) 1700watts Max.
Dimensions (LxWxH)
600mm x 600mm x 264mm
Weight
32kg approx
Operating conditions
Regulatory
10°C - 30°C, 0 - 95% relative
humidity
CE, UL, CSA compliant
Warranty
2 years on all systems
G-Storm Thermal Cyclers
Appendix
53
Compatible Barcodes
The following barcodes are compatible with G-STORM’s documentation
system:
54

2 of 5 interleaved

Codabar

Code 128

Code 39

Code 39 full ASCII

Code 93 full ASCII

EAN-13, EAN -13 with 2 supplement, EAN -13 with 5 supplement

EAN-8, EAN -8 with 2 supplement, EAN -8 with 5 supplement

EAN-128

UPA, UPA -13 with 2 supplement, UPA -13 with 5 supplement

UPE, UPE -13 with 2 supplement, UPE -13 with 5 supplement

MSI

C.I.P
Appendix
G-Storm Thermal Cyclers
Compatible Plate Formats
Gene Technologies recommend using G-Storm consumables with G-Storm
Thermal Cyclers.
Cat. No.
Product description
G-Storm RIGID Plates
GSR0960/C
GSR0961
GS RIGID 96, clear frame, clear wells
GS RIGID 96, Black Frame, white wells
GSR0384/C
GSR0385
GS RIGID 384, clear frame, clear wells
GS RIGID 384, black frame, white wells
G-Seals
GS-0500
Adhesive Clear Seal
GS-0521
Peelable Foil Heat Seal
GS-0536
Piercable Foil Heat Seal
GS-0541
Clear Heat Seal
GS-0550
Adhesive Foil Seal
The following plate formats are compatible with G-Storm thermal cyclers:
55
Appendix
G-Storm Thermal Cyclers
Manufacturer
Art. No. & Product Name
Type
B70511 96 Well thin wall plate
96 well, non skirted
B70661 96 Well thin wall plate
96 well, semi-skirted
B70521 384 Well thin wall plate
384 well skirted
AB 600
96 well, non-skirted
AB 800
96 well, skirted
AB 900
96 well, semi-skirted
AB 1111
384 well
TF-0384
384 well
THERMOSPRINT® 96 PP, transparent,
311296; only w/o workstation
96 well, non-skirted
BioPlastics
Abgene
Bilatec
THERMOSPRINT® 384 PP, transparent, 384 well
310384
NUNC
Art. No.: 240600, 96 well, non-skirted.
96
Art. No.: 230013, 96 well, semi-skirted
96
Art. No.: 230012, 96 well, skirted
96
Art. No.: 230584, 384 well
384
Eppendorf
Art. No.: 0030 128.648, twin tec plate, 96
skirted
Art. No.: 0030 128.575, twin tec plate, 96
semi-skirted
Art. No.: 0030 128.508, twin tec plate,
384
Corning
Thermowell®96
Well
Polypropylene
Microplate, Natural (#6551)
Thermowell™ 96 Well Polycarbonate
Microplate, Model M (#6511)
Corning® 384 Well
Microplate (#6500)
Corning®
384
Well
Microplate (#6502)
56
Appendix
Polyproplyene
Polyethylene
G-Storm Thermal Cyclers
Order Information
Part #
Description
Thermal Cyclers
GS00004M
G-STORM GS4 with Manual Lids
Thermal Blocks
GSB00096
96 well block
GSFAST96
96 well Fast block
GSB00384
384 well block
GSFAST384
384 well Fast block
GS00SITU
In situ block
G-Storm Thermal Cyclers
Appendix
57
Sales & Service
G-Storm.
Somerton Biotechnology Centre
Ricksey Lane
Catcombe
Somerton
Somerset
TA11 7JH
Phone +44 (0) 7919 121510
E-Mail
[email protected]
Visit our website: www.gstormdirect.com
58
Appendix
G-Storm Thermal Cyclers
Index
AC Power Connection, 16
Grounding Requirements., 16
Archiving Reports, 47
G-Storm Logo button, 20
Automatic Hot Start command, 27
Home Page, 13
Barcode Input command, 26
Importing and Exporting Files, 46
Block Exchange, 52
Importing Program Files, 46
Changing Users, 37
Incubate temperature, 36
Cleaning, 48
Incubate button, 19
Command buttons, 19
incubation, 36
Additional functions, 20
Initial Inspection, 10
Cycler Monitor, 20
Installation, 8
G-Storm Logo, 20
Block Requirements, 9
Incubate, 19
Electrical Requirements, 8
Lightning flash, 20
Site Requirements, 8
New Program, 19
Thermal Block, 11
Program Wizard, 19
Instrument Reservation, 19, 38
Reports, 20
Language Selection, 45
Run Program, 19
Lid, 25
Settings, 20
Ligation reactions, 36
View/Edit Program, 19
Lightning flash, 20
Creating Programs, 24
Loading the Cycler, 39
Cycler Installation, 14
Logging in as Administrator, 14
Cycler Management, 21
Login button, 18
Cycler Monitor, 20
Main Screen, 13, 18
Cycler Settings, 23
Maintenance, 48
Cycler tests, 15
Manual Hot Start command, 28
Cycler validation Tests, 15
New Program button, 19
Default Password, 14
New Users, 22
Deleting a Program Step, 35
Operating Power Requirements, 16
Deleting Programs, 35
Operation, 37
DND Programming, 24
Cancelling a Reservation, 38
Editing Programs, 34
Changing Users, 37
End Cycle command, 31
Instrument Reservation, 38
Exporting Program Files, 46
Running a Program, 40
Functional Overview, 12
Sample Loading, 39
Fuses, 16
Terminating a Run, 43
Gradient Step command, 30
User Login, 37
Gradient Step Conversion, 35
Viewing Program Profile, 42
G-Storm Thermal Cyclers
Appendix
59
Viewing Script Temperature Profiles Prior
to Run, 38
60
Symbols and Conventions, 4
Safety Warnings, 3
Optional Devices, 15
Sales & Service Centres, 60
Order Information, 59
Saving Programs, 33
Package Contents, 10
Script Information command, 31
Password, 14
Service and Support button, 18
Program Execution, 40
Setting the Lid Pressure, 40
Program Steps, 26
Settings button, 20
Automatic Hot Start, 27
Software Operation, 17
Barcode Input, 26
G-STORM and GSPC Software, 17
End Cycle, 31
Required Computer Skills, 17
Gradient Step, 30
Specifications, 53
Lid Pressure, 26
Compatible Barcodes, 56
Manual Hotstart, 28
Compatible Plate Formats, 57
Script Information, 31
High Pressure Heated lid, 53
Start Cycle, 31
Neworking & Barcode Options, 55
Store, 31
Power & Dimensions, 55
Temperature Step, 28
Reports and Validation, 54
Touchdown, 29
Thermal Blocks, 53
Program Wizard, 32
Thermal Engine Characteristics, 55
Program Wizard button, 19
User & File Management, 54
Programming, 24
User Interfaces, 54
Converting a Gradient Step, 35
Start Cycle command, 31
Deleting a Program Step, 35
Starting a Run on a Single Cycler, 40
Deleting Programs, 35
Starting a Run on Multiple Cyclers, 41
Editing Programs, 34
Starting up the G-STORM, 14
General Guidelines, 32
Status bar, 19
Program Wizard, 32
Instrument Reservation, 19
Saving Programs, 33
Time and Temperature display, 19
Reports, 44
Store command, 31
Archiving Reports, 47
Switching Off the Instrument, 45
Viewing Reports, 44
Symbols and Conventions, 4
Reports button, 20
System Settings, 21
Requirements, 8
Temperature Step command, 28
Run Program button, 19
Terminating a Run, 43
Running a Program
Thermal Block, 11
Starting a Run on a Single Cycler, 40
Time and Temperature display, 19
Starting a Run on Multiple Cyclers, 41
Title bar, 18
Safety
Login, 18
Hazards, 5
Service and Support, 18
Instrument, 4
Touchdown command, 29
Appendix
G-Storm Thermal Cyclers
Trouble Shooting
Adding or Modifying Accounts, 22
If a Power Failure Occurs, 49
Deleting Accounts, 23
Trouble-Shooting, 49
User Levels, 21
Unpacking the Thermal Cycler, 10
User Login, 37
Uploading Primer Sequences, 47
User Management, 21
USB Memory Stick Operation, 46
View/Edit Program button, 19
User Accounts
Viewing Program Profile, 42
G-Storm Thermal Cyclers
Appendix
61

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