TPersonal user manual

TPersonal user manual
Instruction Manual
Ver. 09/10
Model
TPersonal 20
TPersonal 48
TPersonal Combi
•
Order No.
050-550
050-551
050-552
Please read these instructions carefully before using this
apparatus!
•
Biometra GmbH
Rudolf-Wissell-Str. 30
D-37079 Göttingen
Tel.: ++49 (0)551-50 68 6-0
Fax: ++49 (0)551-50 68 6-66
email: [email protected]
Internet: http://www.biometra.com
Service Department
Rudolf-Wissell-Strasse 14-16
D-37079 Göttingen
Tel.: ++49 (0)551-50 68 6-10 or -12
Fax: ++49 (0)551-50 68 6-11
email: [email protected]
This document describes the state at the time of publishing. It needs not necessarily agree with
future versions.
Subject to change!
2
Instruction Manual TPersonal 10/2009
Contents
1
Introduction......................................................................................................................... 6
1.1
Field of Applications ..................................................................................................... 6
1.2
Special features ............................................................................................................ 6
1.2.1
TPersonal block modules ..................................................................................... 6
1.2.2
TPersonal heating and cooling rates .................................................................... 6
1.2.3
TPersonal operating software............................................................................... 6
1.3
Technical specifications TPersonal Thermocycler........................................................ 7
1.4
Legal Notes .................................................................................................................. 8
1.4.1
PCR License – Legal Disclaimer .......................................................................... 8
1.4.2
Copyright .............................................................................................................. 8
1.4.3
Liability.................................................................................................................. 8
1.4.4
Meaning of the Instructions................................................................................... 8
2
3
4
5
Safety and Warning Notices .............................................................................................. 9
2.1
Definition of Symbols .................................................................................................... 9
2.2
General Safety Instructions .......................................................................................... 9
Installation......................................................................................................................... 12
3.1
Content of delivery...................................................................................................... 12
3.2
Unpack and Check ..................................................................................................... 12
3.3
Installation Conditions ................................................................................................ 12
3.4
Operation Voltage....................................................................................................... 13
3.5
Initial self test.............................................................................................................. 14
Operating elements .......................................................................................................... 15
4.1
The TPersonal front view............................................................................................ 15
4.2
The TPersonal rear view ............................................................................................ 16
4.3
The TPersonal control panel ...................................................................................... 17
4.4
High Performance Smart Lid (HPSL).......................................................................... 18
Operating........................................................................................................................... 19
5.1
The TPersonal display................................................................................................ 19
5.2
Navigation within the TPersonal software................................................................... 19
5.3
Create program .......................................................................................................... 19
5.3.1
Select directory ................................................................................................... 19
5.3.2
Select program store .......................................................................................... 20
5.3.3
Enter program name........................................................................................... 21
5.3.4
Enter lid temperature .......................................................................................... 22
5.3.5
Select / deselect lid pre-heating.......................................................................... 22
5.3.6
Enter temperature and time settings................................................................... 23
5.3.7
Set loop............................................................................................................... 24
5.3.8
Cool below ambient temperature........................................................................ 25
5.3.9
Save program ..................................................................................................... 25
5.4
Edit programs ............................................................................................................. 26
Instruction Manual TPersonal 10/2009
3
5.4.1
Delete program steps ..........................................................................................26
5.4.2
Insert program steps ...........................................................................................27
5.4.3
Copy program......................................................................................................28
5.4.4
Delete program....................................................................................................30
5.5
Further programming options ......................................................................................31
5.5.1
Program time increments ....................................................................................31
5.5.2
Program touch down ...........................................................................................32
5.5.3
Adjust heating and cooling rates .........................................................................32
5.6
Run program ...............................................................................................................32
5.6.1
Select and start program .....................................................................................32
5.6.2
View program prior to start ..................................................................................33
5.6.3
Display during operation......................................................................................34
5.6.4
View remaining run time ......................................................................................34
5.6.5
Pause / stop program ..........................................................................................34
5.7
System settings ...........................................................................................................35
5.7.1
System info..........................................................................................................35
5.7.2
Switch beep on/off...............................................................................................36
5.7.3
Select language...................................................................................................36
5.7.4
Display contrast...................................................................................................36
5.7.5
Diagnostics..........................................................................................................37
6
Short manual .....................................................................................................................38
7
Trouble shooting...............................................................................................................42
7.1
Slow heating and cooling ............................................................................................42
7.2
Restart due to unrecognised power failure..................................................................42
7.3
Adaptation of protocols from other cyclers ..................................................................42
7.4
Lid can not be opened.................................................................................................42
7.5
Releasing wheel in case of blocked lid........................................................................42
8
Maintenance and repair ....................................................................................................44
8.1
Cleaning and Maintenance..........................................................................................44
8.2
Servicing and repair ....................................................................................................44
8.3
Firmware update .........................................................................................................44
8.4
Replacement of Spare Parts .......................................................................................44
9
Accessories .......................................................................................................................45
9.1
10
Plasticware ..................................................................................................................45
Service ...............................................................................................................................46
10.1
Instructions for return shipment...................................................................................46
10.2
Packing of the Thermocycler.......................................................................................47
11
Equipment Decontamination Certificate.........................................................................48
12
Note for the disposal of electric / electronic waste. ......................................................50
13
Declaration of Conformity ................................................................................................51
4
Instruction Manual TPersonal 10/2009
14
Warranty ............................................................................................................................ 52
15
Subject Index .................................................................................................................... 53
Instruction Manual TPersonal 10/2009
5
1 Introduction
1.1
Field of Applications
Today thermocycling is a standard technique in all molecular biology labs. The new Biometra
TPersonal Thermocycler is licensed for PCR applications (research use only) and intended to
be used for performing polymerase chain reactions. The instrument accounts for the increased
demand for flexibility and prevents bottlenecks whenever a limited number of samples have to
be amplified with different protocols. Due to a new memory structure the programming of the
TPersonal Thermocycler is now even easier and delivers highest ease of use to the customer.
1.2
1.2.1
Special features
TPersonal block modules
The TPersonal Thermocycler is available with three different block formats. For standard
applications a 48 well block for 0.2 ml single tubes, strips of 8 tubes or 48 well microplates is
offered. For large volumes a 20 well block for 0.5 ml tubes is available. The combi block offers
maximal flexibility for the use of different sized tubes. The combi block has a capacity for up to
48 x 0.2 ml tubes, strips or plates or 18 x 0.5 ml tubes. When using "small cap tubes" the
capacity increases to 35 x 0.5 ml tubes for the combi block.
1.2.2
TPersonal heating and cooling rates
The TPersonal was developed to meet highest demands. The result is a thermal cycler that
reaches the highest heating and cooling rates in the class of personal thermal cyclers and
excellent temperature uniformity. The high heating and cooling rates ensure specific reaction
conditions and the superior temperature uniformity guarantees that the reaction conditions are
identical in every position of the block.
1.2.3
TPersonal operating software
The TPersonal operating software has been redesigned to ensure easy user management and
clear menu guidance. At the same time the instrument was equipped with a new front panel for
faster navigation through the user menu. The clear user interface in conjunction with the large
screen makes TPersonal Thermocycler an intuitive to operate system.
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Instruction Manual TPersonal 10/2009
1.3
Technical specifications TPersonal Thermocycler
Order number
Model
050-550
TPersonal 20
050-551
TPersonal 48
Block capacity
20 x 0,5 mltubes
48 x 0,2 ml-tubes,
stripes,
48 well-microplates
Max. heating rate*
Max. cooling rate*
Block material
Temperature range
Lid temperature range
Control accuracy
Temperature uniformity
Display
Heated lid
Noise emission
Ports
Memory
Software
Autorestart function
Cool samples at 4°C
Line voltage
Frequency
Power consumption
Fuses
Dimensions (WxDxH)
Storage conditions
Operating conditions
Weight
*
**
050-552
TPersonal combi
18 x 0,5 ml-tubes,**
48 x 0,2 ml-tubes,
stripes,
48 well-microplates
3°C/sec.
3°C/sec.
Aluminium
3°C – 99°C
30°C – 99°C
+ 0,1°C
+ 0,5°C after 15 sec.
High brightness CFL backlight graphical LC Display,
viewing area 124 mm x 34 mm, resolution 256 x 64 dots
High Precision Smart Lid for optimum lid pressure and excellent
temperature uniformity, temperature range 30.0 - 99.0 °C
very low
serial: RS232 (bi-directional data transfer),
port for in tube-sensor
Total capacity: 1500 steps in 10 directories with maximum 100
programs each, max. 99 steps/program, max. 99 cycles
Program steps are easily entered in a spread sheet
Options: time increment, temperature increment, set ramping rate
yes
yes
100 Volt, 115 Volt or 230 Volt
50-60 Hz
max. 260 Watt
see label on the back of the instrument
21,7 x 30,7 x 14,9 cm
5°C –35°C
5°C – 35°C, max. 70% humidity, max. 2000 m NN
6,7 kg
measured inside the block
Capacity increases to 35 x 0.5 ml tubes by use of small cap tubes
Instruction Manual TPersonal 10/2009
7
1.4
Legal Notes
1.4.1
PCR License – Legal Disclaimer
Purchase of a Biometra Thermocycler conveys a limited non-transferable immunity from suit for
the purchaser’s own internal research and development and applied fields other than human in
vitro diagnostics under one or more of US Patents Nos. 5,038,852, 5,656,493, 5,333,675,
5,475,610, and 6,703,236, or corresponding claims in their non-US counterparts, owned by
Applera Corporation.
No right is conveyed expressly, by implication or by estoppel under any patent claim, reagents,
kits, or methods such as 5´ nuclease methods, or under any other apparatus or system claim,
including but not limited to US Patent No. 6,814,934 and its non-US counterparts, which
describe and claim thermal cyclers capable of real-time detection.
Further information on purchasing licenses may be obtained by contacting the Director of
Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Biometra biomedizinische Analytik GmbH
Rudolf Wissell Str. 30
37079 Goettingen, Germany
1.4.2
Copyright
All rights reserved. It is not allowed to copy and publish the manual or parts of it in any form as
copies, micro film or other methods without a written authorisation from Biometra.
Biometra is pointing out that applied company and brand names are usually protected trade
marks.
1.4.3
Liability
Biometra is not liable for damages and injuries caused by use not considering these operating
instructions in parts or completely.
1.4.4
Meaning of the Instructions
Biometra recommends that you first read these instructions carefully. This operation instruction
is part of the product and should be kept over the full life-time of the instrument. It should also
be forwarded to subsequent owners and users. Make sure that additions and updates are
inserted into the operation instructions.
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Instruction Manual TPersonal 10/2009
2 Safety and Warning Notices
2.1 Definition of Symbols
Symbol
Definition
Caution! Refer to instruction manual!
Danger! High voltage!
Fragile!
Danger! Hot surface!
2.2 General Safety Instructions
Please read this manual carefully before starting operation of the TPersonal Thermocycler. The
TPersonal Thermocycler is intended for sample incubation at varying temperatures.
•
General safety precautions for laboratory work must be observed when working with the
TPersonal Thermocycler.
•
The TPersonal does not produce a sound power level that could be hazardous for the user.
The thermoblock and the heated lid will reach high temperatures
during operation. Both thermoblock and heated lid can burn you.
Rapid heating of the thermoblock can cause liquids to boil
explosively. Always wear safety goggles during operation. Close the
lid before starting a program.
Do not heat samples without having the lid locked securely.
Be aware that samples are reaching high temperatures. Do not touch
or open hot tubes or microplates, because hot liquid may quickly spill
out.
Do not touch the heated lid.
Use only suited plastic ware in the TPersonal thermocycler. Tubes
and plates must show good fit when placed in the thermoblock. Only
use tubes that are suited for high temperatures (tight lids).
Instruction Manual TPersonal 10/2009
9
The TPersonal Thermocycler contains no user serviceable parts. Do
not open the housing instrument. Service and repair may only be
carried out by the Biometra Service department or otherwise
qualified technical personal.
Do not use the instrument when damages of the housing, block,
cable or other parts are visible.
Prior to connecting the unit to the power source please ensure that
the voltage selector at the bottom of the instrument is set to the
required voltage.
Make sure that the main supply voltage is in accordance with the
label above the power connection (see section 4.2)
Unplug the power cable before you open the TPersonal
Thermocycler. Danger of electric shock!
Make sure that the appliance connector and the plug of the supply
cord are accessible, so you can separate the instrument from the
mains.
Connect the TPersonal Thermocycler to a grounded socket.
When only few samples are put in the block place additional tubes in
the four corner positions. This is to evenly distribute the lid pressure
and prevents single tubes from excessive pressure. Use of few tubes
may result in damage of the tubes by excessive pressure.
Appropriate safety regulations must be observed when working with
infectious, pathogenic or radioactive material. Ask the responsible
local safety inspector for details.
The TPersonal Thermocycler must not be used with explosive,
flammable or volatile liquids.
Do not place fingers between lid and housing when opening or
closing the lid.
Before opening of the lid, release lid pressure
It is not necessary to apply oil into the opening of the block in order
to improve the heat transfer between the block and the sample
tubes.
If you still decide to use oil, do not use silicon oil. Mineral oil may be
used.
Ensure that both the rear and bottom ventilation slits not clogged by
dust or other material. Danger of overheating!
Let equilibrate the TPersonal Thermocycler to room temperature
before starting operation.
There must be sufficient distance between the ventilation slots on the
rear side of the Thermocycler and a wall or another instrument (min
10 cm). Danger of overheating!
10
Instruction Manual TPersonal 10/2009
This instrument is designed and certified to meet EN 61010-1 safety
standards. It should not be modified or altered in any way. Alteration
of this instrument will void the warranty, void the EN61010-1
certification, and create a potential safety hazard.
Place the TPersonal Thermocycler on a stable, non flammable
surface in a dry, safe environment. For details see working
conditions in table “Technical specifications” (see chapter 1.3).
Do not use alcohol (e.g. methanol, ethanol), organic solvents or
abrasives to clean the instrument.
For transports always use the original Biometra box.
Instruction Manual TPersonal 10/2009
11
3 Installation
3.1 Content of delivery
1)
2)
3)
4)
Thermocycler
Mains connector
Manual
Short Manual
Please keep the original packaging material for return shipment in case of servicing. The
TPersonal shipping box provides a specially developed system for contact-free transport of this
electronic device.
3.2 Unpack and Check
Unpack and carefully examine the instrument. Report any damage to Biometra. Do not attempt
to operate this device if physical damage is present.
Please keep the original packing material for return shipment in case of service issues
!! Attention !!
Please fill out and send back the warranty registration card. This is important for you to
claim full warranty.
3.3 Installation Conditions
•
Place the TPersonal Thermocycler on a stable surface in a dry, safe environment. For
details see working conditions in table “Technical specifications” (see chapter 1.3).
•
Let equilibrate the TPersonal Thermocycler to room temperature before starting operation (1
to 6h).
•
Make sure that the appliance connector and the plug of the supply cord are accessible, so
you can separate the instrument from the mains.
•
Make sure that the ventilation slots on the bottom and the rear are not obstructed. Make
sure that there is no object underneath the thermocycler that may block the ventilation slots
at the bottom (e.g. a piece of paper etc.)
•
There must be sufficient distance between the ventilation slots on the rear side of the
Thermocycler and a wall or another instrument (min 10 cm).
Ensure that both the rear and bottom ventilation slits of the
rear and bottom of the instrument are unobstructed.
Insufficient ventilation can cause overheating of the
instrument.
12
Instruction Manual TPersonal 10/2009
•
Make sure that the main supply voltage is in accordance with the label above the power
connection (see section 3.4)
•
Connect the TPersonal Thermocycler to a grounded socket.
Prior to connecting the unit to the power source please ensure that the
voltage selector at the back side of the instrument is set to the required
voltage.
Danger of electric shock! Unplug the power cable before you open the
TPersonal Thermocycler.
•
The display contrast can be adjusted to local light conditions (5.7.4)
3.4 Operation Voltage
The TPersonal Thermocycler can operate at 100, 115 or 230 Volt.
Important: Prior to connecting the TPersonal to the mains, make sure that the setting of the
Voltage selector at the bottom of the instrument is in accordance with your mains Voltage and
that it is equipped with the correct fuses.
1. To change the operation Voltage settings switch off the instrument and disconnect the mains
plug.
2. Use a coin or another round shape item to turn the adjustment slot of the Voltage selector at
the bottom side of the instrument (see picture 1) to the new Voltage setting.
3. Pull out the fuse holder at the backside of the TPersonal (see picture 2). Load the fuse holder
with two fuses corresponding to the designated operation Voltage. At an operation Voltage of
230V use two slow-blow fuses of 2 Ampere (2x 2ATT), at an operation Voltage of 100 or 115
Volt use two slow-blow fuses of 4 Ampere (2x 4ATT).
4. Reinsert the fuse holder.
5. Switch on the instrument.
Instruction Manual TPersonal 10/2009
13
3.5
Initial self test
After switching on the TPersonal the serial number of the instrument and the software version is
displayed.
T Personal
Serial number
Block type
Vers.
(c) biometra 2008
The TPersonal then checks all programs in all subdirectories (so called RAM check).
T Personal
Serial number
Block type
Checking directory 7 program 4
14
Instruction Manual TPersonal 10/2009
4 Operating elements
4.1
The TPersonal front view
Lid wheel for
adjustment of lid
pressure
Lid pressure
lock
Keypad
Display
Power switch
Instruction Manual TPersonal 10/2009
Context sensitive
menu buttons
15
4.2
The TPersonal rear view
Easy to clean
operating surface
Power cord plug
Fuse holder for 2 fuses, for
specification of fuses see
type label on the instruments
backside
16
Instruction Manual TPersonal 10/2009
4.3
The TPersonal control panel
Easy to clean
operating surface
Power switch
Display
Context sensitive
menu buttons
Instruction Manual TPersonal 10/2009
Cursor keys
Keypad
17
4.4 High Performance Smart Lid (HPSL)
To achieve optimum pressure on the tubes the TPersonal is equipped with a height adjustable
heated lid.
Close the lid:
After the samples have been placed in the block close the lid. Turn the wheel clockwise until
you hear a clicking noise. In this mode the pressure will not increase further, even when you
keep on turning the wheel.
Note: The pressure of the lid has been optimized for a fully loaded block. If only very few tubes
are loaded to the block you should place dummy tubes in the four corner positions to avoid
damage of tubes by excessive pressure.
Open the lid:
First: Release pressure by turning the wheel counter clockwise. As soon as there is no more
resistance the pressure has been released.
Then: Open the lid.
Important: The lid should not be opened under pressure because this leads to damage of the
locking mechanism.
18
Instruction Manual TPersonal 10/2009
5 Operating
5.1
The TPersonal display
The TPersonal display provides information about the instrument and about the status of the
active program.
Prog
Temp
23.9°C
Deckel
Info
24.0°C
System
Start/Stop
Edit
The function of the four soft keys beneath the display is context sensitive. The respective
function is shown in the display (see also chapter 4.3). The function of each key can differ from
screen to screen.
5.2
Navigation within the TPersonal software
The TPersonal provides spread sheet programming. This means that all program parameters
are entered into a simple central spreadsheet.
Four navigation keys provide easy navigation within the software. The back and the forward key
have additional functions as described below:
The right cursor key moves the cursor to the next field.
This cursor can also be used to complete data entry. By pressing the right
cursor settings will be saved and the cursor moves to the next field.
In the file directory, this key moves to cursor forward to the next (lower) level.
The left cursor key moves the cursor back to the previous field.
In most screens this cursor is equivalent to the ”back” softkey.
In the file directory, this key moves the cursor back to the higher level.
5.3
Create program
Prior to writing a new program, a program store has to be chosen. The TPersonal provides 10
directories of which each can store up to 99 different programs. For easy identification of the
selected storage place, the directories can be named (see section 5.3.3). The total memory
capacity is 1.500 program steps.
5.3.1
Select directory
Start with the main screen.
Instruction Manual TPersonal 10/2009
19
Prog
Temp
23.9°C
Lid
Info
24.0°C
System
Start/Stop
Edit
Press [Edit] to enter the edit mode.
- 0:
- 1:
- 2:
- 3:
- 4:
Page ÇÈ
Edit program
Name
Back
Enter
You are now in the directory structure of the edit mode.
Use cursor keys to select a subdirectory.
- 0:
- 1:
- 2:
- 3:
- 4:
Page ÇÈ
Edit program
Name
Back
Enter
Press [Enter] to enter highlighted subdirectory. The program storages are displayed.
- 3: -----------Edit program
Page Ç
5.3.2
Page È
0: empty
1: empty
2: empty
3: empty
4: empty
Copy/Del.
Edit
Select program store
Use cursor keys to select a program store.
20
1
2
3
4
5
6
Tip: Use numeric keypad to directly enter program number.
7
8
9
For numbers smaller than 10 enter “0” plus number.
.
0
-
Instruction Manual TPersonal 10/2009
- 3: -----------Edit program
Page Ç
Page È
0: empty
1: empty
2: empty
3: empty
4: empty
Copy/Del.
Edit
Using the softkeys [Page Ç] and [Page È] you can scroll through the list in 5 program steps.
Press [Edit] or Æ to write new program in Program store 03.
5.3.3
Enter program name
Each program is specified by a program number and a subdirectory number. To make retrieval
of a program easier, you can enter a name for each program existing of letters, numbers and
symbols.
- 3: -------------- 3:
Å Del.
Blank
∧
ABCDEFGHIJKLM
NOPQRSTUVWXYZ
-()#C/,〈〉&+.%!
Name OK
Enter
Use cursor keys to select a letter.
Accept letter with [Enter].
- 3: -------------- 3:
Å Del.
Blank
B
∧
ABCDEFGHIJKLM
NOPQRSTUVWXYZ
-()#C/,〈〉&+.%!
Name OK
Enter
Use cursor keys to select next letter.
Accept letter with [Enter]. Repeat until name is completed.
Note: Press [Å Del.] to delete a false letter. You may also use certain special characters to
name the folder. To enter a blank press the [Blank] button.
Note: Do not use special characters if you would like to control the TPersonal Thermocycler by
the Thermocycler Manager Software.
Instruction Manual TPersonal 10/2009
21
- 3: -------------- 3:
Blank
Å Del.
BIOMETRA
∧
ABCDEFGHIJKLM
NOPQRSTUVWXYZ
-()#C/,〈〉&+.%!
Name OK
Enter
Once the name is completed, confirm name with [name OK].
- 3: -------------- 3: BIOMETRA
Lid temp ----C
Preheating On
Info
5.3.4
Page È
Save Pgm
Enter
Enter lid temperature
You can enter a temperature for the heated lid in a range of 30 °C to 99 °C.
Note: Thanks to a new lid design, significantly lower lid temperatures than in the past can be
used for thermocycling. The lower lid temperature leads to a higher temperature uniformity
within the lid and thus to a more even temperature distribution in the tubes. For instruments
featuring the new lid design, a maximum temperature of 99.0°C should can used.
- 3: -------------- 3: BIOMETRA
Lid temp 99.0C
Preheating
On
Info
5.3.5
Page È
Save Pgm
Enter
Select / deselect lid pre-heating
You can choose whether the lid is pre-heated before the program starts. This is to avoid
evaporation during the initial heating phase.
Note: During the preheating of the lid, the block is held constant at 25°C.
- 3: -------------- 3: BIOMETRA
Lid temp 99.0C
Preheating
On
Info
Page È
Save Pgm
1. Enter
Note: The default setting, which is recommended for most applications, is preheating: On.
After all pre-settings have been made, press [Enter] to open the programming spreadsheet.
22
Instruction Manual TPersonal 10/2009
5.3.6
Enter temperature and time settings
The programming spreadsheet:
Temp[C]
1:
2:
3:
4:
Info
Time
Insert/Del
←
#
Options -->
Save Pgm
Enter
Note: In this spreadsheet you can enter all parameters for your cycling protocol. In addition, you
can set special parameters like touch down, ramping rates or time increments. For details on
these special parameters, see section 5.4.1.
You can navigate in the spreadsheet without limitations with the four
cursor keys
Note: Each setting is confirmed with [Enter]. The cursor moves automatically to the next field.
Alternatively, you can confirm a value by moving forward with the cursor keys.
Note: At any time you can activate the help function with [Info]
Note: In an existing protocol program steps can be deleted and inserted. For further information
on deleting and inserting program steps (see sections 5.4.1 and 5.4.2).
Now enter temperature for the first step and press [Enter]. In the next row you can enter the
time for this temperature:
Temp[C]
1: 95.0
2:
3:
4:
Info
Time
Insert/Del
←
#
Options -->
Save Pgm
Enter
Note: There is a convention on how time settings are entered in all BIOMETRA cyclers:
hours z minutes z seconds
If you enter a number without “dot” this value will be interpreted as seconds (“300” => 5
minutes). To program minutes enter a “z” after the number of minutes. To enter hours enter z
z after the number. You can also enter any combination of hours, minutes and seconds.
Example: for 1 hour, 30 minutes, 20 seconds enter 1z z 30 z 20.
The time values will be displayed in the following format: 0h 00 m 00s
Note: To program a pause press “0” and [Enter]. For the corresponding step “Pause” will be
displayed. To manually pause a program see section 5.3.8.
Instruction Manual TPersonal 10/2009
23
Enter “5” “dot” for 5 minutes initial denaturation. Press [Enter].
Temp[C]
1:
95.0
2:
3:
4:
Info
←
Time
#
Options -->
0h 5m 0s
Insert/Del
Save Pgm
Enter
Repeat for all temperature steps in the program.
5.3.7
Set loop
In general, loops are defined by two parameters:
1) The target for the back loop (row „←“)and
2) The number of back loops (row „#“).
Temp[C]
1:
95.0
2:
95.0
3:
55.0
4:
72.0
Info
Time
0h 5m 0s
0h 1m 0s
0h 1m 0s
0h 1m 0s
Insert/Del
←
#
Options -->
Save Pgm
Enter
In the row labelled with Å enter the target step for a back loop and press [Enter].
Temp[C]
1:
95.0
2:
95.0
3:
55.0
4:
72.0
Info
#
Options -->
←
0h 5m 0s
0h 1m 0s
0h 1m 0s
0h 1m 0s 2
Insert/Del
Save Pgm
Enter
Time
In the row labelled with # you can enter the number of back loops.
Note: total cycler number = (n back loops) + 1, e.g. enter 29 for a total number of 30 cycles.
Temp[C]
1:
95.0
2:
95.0
3:
55.0
4:
72.0
Info
Time
0h 5m 0s
0h 1m 0s
0h 1m 0s
0h 1m 0s
Insert/Del
←
#
2
24
Save Pgm
Options -->
Enter
This protocol now contains 25 cycles between step 2 (95.0°C for 1 minute) and step 3 (55.0 °C
for 1 minute).
24
Instruction Manual TPersonal 10/2009
5.3.8
Cool below ambient temperature
Temp[C]
3:
55.0
4:
72.0
5:
72.0
6:
16.0
Info
Time
0h 1m 0s
0h 1m 0s
0h 5m 0s
Pause
Insert/Del
←
#
2
24
Save Pgm
Options -->
Enter
To hold the temperature for an indefinite time enter “0”. After pressing [Enter] “pause” is
displayed.
Note: If a program ends with a pause it has to be manually stopped by the user (see section
5.6.5).
Note: Biometra recommends to cool samples at a temperature of 16°C rather than 4°C. This
prolongs the life span of the instrument.
5.3.9
Save program
Save program with [Save Pgm]. The program data are written to the permanent memory.
Temp[C]
3:
55.0
4:
72.0
5:
72.0
6:
16.0
Info
Time
0h 1m 0s
0h 1m 0s
0h 5m 0s
Pause
Insert/Del
←
#
2
24
Save Pgm
Options -->
Enter
Confirm changes with [Yes]. The run time is calculated.
- 3: -------------- 3: BIOMETRA
Save changes?
Cancel
- 3: -------------- Yes
No
3: BIOMETRA
Number of steps: 6
Run time:
1h13m46
>>> Saving program.<<<
Note: The run time is calculated according to the block type. Please be aware that different
block types provide different ramping rates (see section 1.3, Technical specifications)
Note: Please note that time increments (see section 5.5.1) and altered heating and cooling
rates (see section 5.5.3) have an influence on the overall run time.
Instruction Manual TPersonal 10/2009
25
Prog
Temp
23.9°C
Lid
Info
24.0°C
System
Start/Stop
Edit
Note: To view the remaining runtime during a run press [Info] in the main menu.
5.4
Edit programs
Select program as described in section 5.3.1 and section 5.3.2.
Note: During operation of the cycler the active program can be viewed but not modified. If you
want to change settings of the active program for further experiments you have to save a copy
of this program to another memory. For further information about copying programs see section
5.4.3.
5.4.1
Delete program steps
Open the programming spreadsheet:
Temp[C]
1:
95.0
2:
95.0
3:
55.0
4:
72.0
Info
←
Time
0h 5m 0s
0h 1m 0s
0h 1m 0s
0h 1m 0s
Insert/Del
#
2
24
Save Pgm
Options -->
Enter
To delete a program step press [Insert/Del]
Insert / Delete step:
1:
95.0
0h 5m
2:
95.0
0h 1m
3:
55.0
0h 1m
4:
72.0
0h 1m
Cancel
Å Del.
0s
0s
0s
0s
2
24
Delete
Insert
Enter the number of the step that you want to delete and press [Delete].
Example: To delete step 3 enter “3” and press [Delete]
Insert / Delete step:
1:
95.0
0h 5m
2:
95.0
0h 1m
3:
55.0
0h 1m
4:
72.0
0h 1m
Cancel
Å Del.
26
3
0s
0s
0s
0s
2
24
Delete
Insert
Instruction Manual TPersonal 10/2009
Insert / Delete step: 3
1:
95.0
0h 5m 0s
2:
95.0
0h 1m 0s
3:
55.0
0h 1m 0s
4:
72.0
0h 1m 0s 2
>>> Deleting step 3... <<<
24
The corresponding step is deleted.
Temp[C]
1:
95.0
2:
95.0
3:
72.0
4:
72.0
Info
Time
0h 5m 0s
0h 1m 0s
0h 1m 0s
0h 5m 0s
Insert/Del
←
#
2
24
Options -->
Save Pgm
Enter
Note: The insertion or deletion of steps may have an influence on loops. If for a deleted step a
loop was defined, the loop will be deleted too. Please insure that the modified program will work
correctly.
5.4.2
Insert program steps
Open the programming spreadsheet:
Temp[C]
1:
95.0
2:
95.0
3:
55.0
4:
72.0
Info
←
Time
0h 5m 0s
0h 1m 0s
0h 1m 0s
0h 1m 0s
Insert/Del
#
Options -->
2
24
Save Pgm
Enter
To insert a program step press [Insert/Del]
Insert / Delete
1:
95.0
2:
95.0
3:
55.0
4:
72.0
← Del.
step:
0h 5m
0h 1m
0h 1m
0h 1m
0s
0s
0s
0s
2
Cancel
24
Delete
Insert
Enter the number of the step that you want to insert / delete.
Example: To insert new step at step 3, enter “3” and press [Insert]
Insert / Delete step:
1:
95.0
0h 5m
2:
95.0
0h 1m
3:
55.0
0h 1m
4:
72.0
0h 1m
Cancel
Å Del.
Instruction Manual TPersonal 10/2009
3
0s
0s
0s
0s
2
24
Delete
Insert
27
Press [Insert]
Insert / Delete step: 3
1:
95.0
0h 5m 0s
2:
95.0
0h 1m 0s
3:
55.0
0h 1m 0s
4:
72.0
0h 1m 0s 2
24
>>> Inserting new step at step 3... <<<
A new step is inserted (Temp 10°C, Time 0h 0m 1s).
Enter temperature and time for new step.
Temp[C]
1:
95.0
2:
95.0
3:
10.0
4:
55.0
Info
←
Time
0h 5m 0s
0h 1m 0s
0h 0m 1s
0h 1m 0s
Insert/Del
#
Options -->
Save Pgm
Enter
Note: Additional steps will be inserted before the selected step. The following steps are shifted
by one position. The new step has been entered within an existing loop, this loop now consists
of three steps.
Note: The insertion and deletion of steps may have an influence on existing loops. Be sure that
all settings are updated to maintain a correct cycle.
5.4.3
Copy program
Select the program you want to copy as described in section 5.3.1 and section 5.3.2.
- 3: -----------Edit program
Page Ç
Page È
0: TEST1
1: TEST2
2: BIOMET1
3: BIOMET2
4: BIOMET3
Copy/Del.
Edit
Press [Copy/Del.]
- 3: -------------
Copy Pgm
Delete Pgm
3: BIOMET2
Back
To copy this program press [Copy Pgm]
28
Instruction Manual TPersonal 10/2009
- - - - - Info
Target
0:
1:
2:
3:
4:
Page ÇÈ
Back
Enter
Select the subdirectory with the cursor keys, or enter number on the keypad:
- - - - - Info
Target
0:
1:
2:
3:
4:
Page ÇÈ
Back
Enter
Press [Enter]
- 2: -----------Target
Page Ç
Page È
0: empty
1: empty
2: empty
3: empty
4: empty
Back
Edit
Select program number for the program copy and confirm with [Enter]:
Copy
- 3: ---------To
- 2: ----------
3: BIOMET2
3: empty
Back
Copy
Press [Copy]
Copy
- 3: ---------
To
- 2: ---------
>>> Copying program... <<<
- 2: -----------Edit program
Page Ç
Instruction Manual TPersonal 10/2009
Page È
3: BIOMET2
3: empty
0: empty
1: empty
2: empty
3: BIOMET2
4: empty
Copy/Del.
Edit
29
Note: If the program is copied to an already used storage space the existing program will be
overwritten!
5.4.4
Delete program
Select the program you want to delete as described in section 5.3.1 and section 5.3.2.
- 3: -----------Edit program
Page Ç
Page È
0: TEST1
1: TEST2
2: BIOMET1
3: BIOMET2
4: BIOMET3
Copy/Del.
Edit
Press [Copy/Del.]
- 3: -------------
Copy Pgm
Delete Pgm
3: BIOMET2
Back
To delete this program press [Delete pgm]
- 3: -------------
3: BIOMET2
Delete program?
Cancel
Delete
Note: Once a program has been deleted, it cannot be restored!
Press [Delete]
- 3: -------------
3: BIOMET2
>>> Deleting program... <<<
In the directory structure the program is displayed as empty.
- 3: -----------Edit program
Page Ç
30
Page È
0: TEST1
1: TEST2
2: BIOMET1
3: empty
4: BIOMET3
Copy/Del.
Edit
Instruction Manual TPersonal 10/2009
You can enter a new program.
To get back to the main screen, 2 x press cursor key [Å]
5.5
Further programming options
Open the programming spreadsheet:
Temp[C]
1:
95.0
2:
95.0
3:
55.0
4:
72.0
Info
Time
0h 5m 0s
0h 1m 0s
0h 1m 0s
0h 1m 0s
Insert/Del
←
#
Options -->
2
24
Save Pgm
Enter
Note: To program special parameters you can scroll the display to the right.
Move the cursor to the right side row of the display (Options Æ).
Main funct.
1: Å
2: Å
3:
Å
4:
Å
Info
dT[C]
Insert/Del
dt[s]
Save Pgm
°C/s
3.00
3.00
3.00
3.00
Enter
Note: To go back to programming sheet, move the cursor to the very left side of the display
(row “Main funct.”).
Alternatively you can move the cursor to the very right.
Temp[C]
1:
95.0
2:
95.0
3:
55.0
4:
72.0
Info
Time
0h 5m 0s
0h 1m 0s
0h 1m 0s
0h 1m 0s
Insert/Del
←
#
Options -->
+
2
24
Save Pgm
Enter
Note: In the main screen you will find the information that a special parameter has been
modified. This is indicated by a + in the row labelled with opt Æ.
5.5.1
Program time increments
To compensate for loss in enzyme activity each step within a loop can be extended from cycle
to cycle. Enter the desired time increment [seconds] in the row labelled with dt[s]. This value will
be added to the time value from cycle to cycle.
Main funct.
1: Å
2: Å
Instruction Manual TPersonal 10/2009
dT[C]
dt[s]
Ê[°/s]
3.00
3.00
31
3:
4:
Info
Å
Å
10
Insert/Del
3.00
3.00
Save Pgm
Enter
Note: A time increment will have an impact on the total runtime depending on the numbers of
cycles and the size of the increment. A program with many cycles and large time increments will
take a significantly longer time than a standard protocol.
Note: A time increment has to be programmed within a loop. Otherwise the entered increment
will be without an effect.
5.5.2
Program touch down
For some applications it is useful to start with a higher temperature and to decrease this
temperature from cycle to cycle. This subsequent lowering of a temperature is called touch
down.
To decrease the temperature from cycle to cycle enter a negative temperature increment in the
row labelled with dT[s].
Main funct.
1: Å
2: Å
3:
Å
4:
Å
Info
dT[C]
- 0.10
Insert/Del
Ê[°/s]
3.00
3.00
3.00
3.00
Save Pgm
Enter
dt[s]
Note: Be sure that the temperature decrease is set in a step that lies within a loop. Otherwise
there will be no iterative temperature decrease.
5.5.3
Adjust heating and cooling rates
Since the TPersonal is a fast thermocycler it may be necessary to reduce the heating and
cooling rate for some applications. For example this is helpful if protocols from other
Thermocyclers shall be used.
The heating or cooling rate respectively can be set in the row labelled with Ê[°/s].
Main funct.
1: Å
2: Å
3:
Å
4:
Å
Info
dT[C]
Insert/Del
Ê[°/s]
3.00
1.00
3.00
3.00
Save Pgm
Enter
dt[s]
Note: The default setting [3.00 °/s] indicates maximum ramping.
5.6
5.6.1
Run program
Select and start program
Open the main screen:
32
Instruction Manual TPersonal 10/2009
Prog
Temp
23.9°C
Lid
Info
24.0°C
System
Start/Stop
Edit
To run a program press [Start/Stop]
- 0:
- 1:
- 2:
- 3:
- 4:
Page ÇÈ
Start block
Direct
Back
Enter
Select directory with cursor keys [Ç] [È] or enter directory number and press [Enter].
- 2: -----------Edit program
Page Ç
Page È
0: BIOMET1
1: BIOMET1
2: BIOMET1
3: BERND2
4: BIOMET1
View
Start
Select program with cursor keys or enter program number.
Note: To be sure that the correct program is selected, it can be displayed by pressing [View]
(see section 5.6.2).
Press [Start] to run program.
5.6.2
View program prior to start
- 2: -----------Edit program
Page Ç
Page È
0: BIOMET1
1: BIOMET1
2: BIOMET1
3: BERND2
4: BIOMET1
View
Start
To check a program prior to start, it can be displayed by pressing [View]
- 2: ------------
3: BERND2
Lid temp
Preheating
Page È
Back
99.0C
Off
Start
Scroll through the program with [Page Ç] and [Page È].
Instruction Manual TPersonal 10/2009
33
Temp[C]
1:
95.0
2:
95.0
3:
55.0
4:
72.0
Time
0h
0h
0h
0h
5m
1m
1m
1m
0s
0s
0s
0s
←
#
2
24
Back
Page ÇÈ
Options -->
Start
To run program press [Start].
5.6.3
Display during operation
During preheating of the lid the following screen is displayed:
Prog
Step
Temp
Time
Lid
Info
3 Biometra
Dir
2
Cycle
25.0 Æ 62.0°C
1
1 of 6
Preheating/55.0°C
System
Start/Stop
Edit
Note: While lid preheating, the block is held constantly at 25.0°C. The program starts as soon
as the lid has reached the set temperature. During the preheating phase the display of the lid
temperature alternates between the current lid temperature and “preheating”.
5.6.4
View remaining run time
Display during operation:
Prog
Step
Temp
Time
Lid
Info
3 Biometra
1
Dir
2
Cycle
1 of 6
25.0°C Æ 62.0°C
0h4m0s Æ 0h5m0s
99.0°C
System
Start/Stop
Edit
To view remaining run time press [Info].
Prog
3 Biometra
Dir
2
Additional information about active pgm
Status:
Step
1
Time
Cycle
1 of 56
Remaining time: 43h34m
Back
The current remaining run time id displayed in line 4.
5.6.5
Pause / stop program
Display during operation:
34
Instruction Manual TPersonal 10/2009
Prog
Step
Temp
Time
Lid
Info
3 Biometra
Dir
2
Cycle
1 of 6
25.0°C Æ 62.0°C
0h4m0s Æ 0h5m0s
99.0°C
System
Start/Stop
Edit
1
To stop or pause a program press [Start/Stop]
Stop/Pause
Directory :
Program No :
2
3 Biometra
Info
Pause
Back
Stop
Press [Pause] to pause block
Press [Stop] to stop block
Note: The program pauses in the current state. The word “Pause” is displayed and alternates
with the elapsed time. You can continue the run with [Start/Stop].
5.7
System settings
Open the main screen:
Prog
Temp
23.9°C
Lid
Info
98.0°C
System
Start/Stop
Edit
Back
Enter
To change instrument settings press [System]
1
2
3
4
5
System-Info
Signal
Language/Sprache
Display contrast
Diagnostics
Page ÇÈ
Scroll though the list with the cursor keys [Ç] [È]. Enter selected function with [Enter].
5.7.1
System info
Select System info, Press [Enter]
Instruction Manual TPersonal 10/2009
35
TPersonal
Serial No 123456
Blocktype
Vers.
© Biometra 2008
Back
This page shows the serial number of the instrument and software version.
5.7.2
Switch beep on/off
There is an option for an audible signal whenever a program enters a pause and when the
program is finished. If this option is active, the beep can be switched off by pressing any key of
the keyboard.
Select signal, press [Enter]
With signal?
Info
5.7.3
Yes
NO
Cancel
Enter
Select language
The display of the TPersonal can be switched from English to German.
Select Language/Sprache, Press [Enter]
Language/Sprache:
Info
German/Deutsch
English/Englisch
Cancel
Enter
To confirm your selection press [Enter]
5.7.4
Display contrast
The display contrast can be set in 4 levels to achieve optimum visibility under individual light
conditions.
Select display contrast, press [Enter]
36
Instruction Manual TPersonal 10/2009
Contrast (0-4 or arrows):
Info
2
Cancel
Enter
User cursor keys to in/decrease contrast value or directly enter setting.
The default setting is “2” which meets average conditions.
5.7.5
Diagnostics
The diagnostic screen contains internal information used by the Biometra service department
for servicing.
Instruction Manual TPersonal 10/2009
37
6 Short manual
Safety warnings
Do not open the instrument unless you are authorised to do so. Check the label at the
backside if the instrument has correct voltage configuration (100V, 115 V or 230 V).
The thermoblock and the heated lid will reach high temperatures during operation. Both
thermoblock and heated lid can burn you.
Rapid heating of the thermoblock can cause liquids to boil explosively. Always wear
safety goggles during operation. Close the lid before starting a program.
It is not necessary to apply oil into the opening of the block in order to improve the heat
transfer between the block and the sample tubes. If you still decide to use oil, do not
use silicon oil. Mineral oil may be used.
Ensure that both the rear and bottom ventilation slits at the rear and bottom of the
instrument are unobstructed. Insufficient ventilation can cause overheating of the
instrument. Tip: At regularly intervals clean the bottom's ventilation slits from dust to
ensure maximum venting capability.
The TPersonal control panel
Display
Power switch Context sensitive Cursor keys
menu buttons
Keypad
TPersonal main screen
Prog
Temp
Lid
23.9°C
24.0°C
Info
System
Softkey 1
Softkey 2
Start/Stop
Edit
Softkey 3
Softkey 4
 The function of the four soft keys beneath the display is context sensitive. The respective function is shown in the
display. The function of each key can differ from screen to screen.
Create, modify and save programs
 Press [Edit] to enter the edit mode.
 Select a user directory with the cursor keys Ö×Ø. Æ Press [Enter]
 Select a program with cursor keys or by number. Æ Press [Edit]
38
Instruction Manual TPersonal 10/2009
Enter program name
- 3: --------------
Blank
3:
∧
ABCDEFGHIJKLM
NOPQRSTUVWXYZ
-()#C/,〈〉&+.%!
Name OK
Enter
Å Del.
 Select letter with cursor keys and accept with [Enter]
 Accept name with [Name OK]
Enter lid temperature
- 3: --------------
3: BIOMETRA
Lid temp 99.0C
Preheating On
Info
Page ↓
Save Pgm
Enter
 Set lid temperature for the Biometra High Performance Lid a few degrees higher than the highest block
Â
Â
temperature programmed. For most applications like PCR, a lid temperature of 99.0°C is recommended. Confirm
with [Enter].
Accept preheating mode “on” Æ block starts after the lid has reached the programmed temperature.
Note: Prior to block start, there is a 40 sec temperature equilibration
Press [Enter]
The programming spreadsheet
Temp[C]
1:
95.0
2:
95.0
3:
55.0
4:
72.0
Info
Time
0h 5m 0s
0h 1m 0s
0h 1m 0s
0h 1m 0s
Insert/Del
←
#
Options -->
+
2
24
Save Pgm
Enter
 Use cursor keys ÕÖ×Ø to navigate in the spreadsheet. Press repeatedly cursor key Ö to open a spreadsheet
extension for programming time and temperature increments.
 Enter temperature and time for each step and confirm with [Enter].
Â
Â
Hours, minutes and seconds are separated by dot (•). To enter for example 1 minute 30 seconds type “1” • “30”.
To enter 1 hour type “1” followed by two • •.
Set loop: Enter the target step for a back loop in column “Å”. The number of back loops (total cycle number –1)
is entered in column “#”. Confirm with [Enter].
To program a pause enter zero. “Pause” will be displayed after moving forward to the next step:
Temp[C]
3:
55.0
4:
72.0
5:
72.0
6:
16.0
Info
Time
0h 1m 0s
0h 1m 0s
0h 5m 0s
Pause
Insert/Del
←
#
2
24
Save Pgm
Options -->
Enter
 To enter temperature or time increments or to alter the ramp rate open spreadsheet extension (press repeatedly
Ö):
Instruction Manual TPersonal 10/2009
39
Main funct.
1:
Å
2:
Å
3:
Å
4:
Å
Info
dT[C]
- 0.10
Insert/Del
Ê[°/s]
3.00
3.00
10
1.00
3.00
Save Pgm
Enter
dt[s]
 To set a temperature or time increment enter the increment value in columns “dT[C]” (temperature) or “dt[s]”
Â
Â
(time). Values have to be entered with a minus sign if temperature or time shall be reduced step by step. Confirm
with [Enter].
Note: Temperature or time increments have to be set within a loop otherwise they will be without effect.
To alter the ramp rate enter new ramping temperature in column Ê[°/s].
Save program with [Save Pgm].
Start program
 Press [Start/Stop] the main screen
 Select directory and program with cursor keys or enter program number.
 Optional: To control program prior to start press [View] and use [Page ÈÇ] or the cursor keys × and Ø to
navigate blockwise or stepwise within the spreadsheet.
 Start selected program with [Start].
Note: After the High Performance Lid has reached the programmed temperature, there is a 40 second
equilibration phase before the program starts (preheating mode “on”).
Display during operation
Prog
Step
1
Temp
Time
Lid
Info
3 Biometra
Dir
2
Cycle
25.0 Æ 62.0°C
1 of 6
Preheating/55.0°C
System
Start/Stop
Edit
Note: When the preheating function is activated, the block is held constantly at 25.0°C when the block is heated up.
During the preheating phase the display of the lid temperature alternates between the current lid temperature and
“preheating”.
 Press [Info] for remaining run time. Press [Exit] to leave the info screen.
 To stop/pause active program press [Start/Stop]
Stop/Pause
Directory :
Program No :
Info
2
3 Biometra
Pause
Back
Stop
 Press [Pause] to pause block
 Press [Stop] to stop block.
Note: The program pauses in the current state. The word “Pause” is displayed and alternates with the elapsed
time. You can continue the run with [Start/Stop].
System settings
 Press [System] in the TPersonal main screen.
40
Instruction Manual TPersonal 10/2009
1
2
3
4
5
System-Info
Signal
Language/Sprache
Display contrast
Diagnostics
Page ÇÈ
Back
Enter
 Select topic by using [Page ÈÇ] or the cursor keys × and Ø and press [Enter].
1. System-Info: Shows information on the serial number of the instrument and the software version.
2. Signal: Switch beep on/off
3. Language/Sprache: The display of the TPersonal can be switched from English to German.
4. Display contrast: Adapt contrast to the local light conditions
Diagnostics: The diagnostic screen contains internal information used by the Biometra service department for
servicing.
Instruction Manual TPersonal 10/2009
41
7 Trouble shooting
7.1
Slow heating and cooling
The TPersonal is equipped with a strong ventilator for the cooling of the heat sink. The inlet of
this fan is located at the bottom side of the instrument. Be sure that the inlet is not clogged by
dust or other material (e.g. a sheet of paper placed under the cycler can be attached to the inlet
as the fan is in operation). Dust can be removed easily from the inlet with a conventional
vacuum cleaner.
7.2
Restart due to unrecognised power failure
High voltage fluctuation can lead to an automatic restart of the thermocycler. In this case the
cycler restarts at the step where there power failure has occurred. To avoid voltage fluctuation,
do not connect the cycler to a socket shared by a strong power consumer like a refrigerator or a
centrifuge.
7.3
Adaptation of protocols from other cyclers
Since the TPersonal is a fast instrument it may be necessary to reduce the heating and cooling
ramps to run protocols from other cyclers. For the setting of the heating and cooling ramps see
section 5.5.3. Alternatively, the time settings may be extended.
7.4
Lid can not be opened
The lid of the TPersonal is equipped with a snap mechanism that arrests when the lid is pressed
down. When it is pressed down again the snap mechanism unlocks and the lid can be opened.
As soon as the heated lid has been lowered by using the lid wheel the lid can not be opened
and first the pressure has to be taken from heated lid. Turn the lid wheel counter clockwise to
relieve the pressure (see section 4.4) and open the lid.
7.5
Releasing wheel in case of blocked lid
Note: When the lid is in the extreme up or down position, it may happen that the wheel is
uncoupled. In this situation the clutch mechanism is active in both directions (clicking noise in
either direction).
To unlock wheel, press down metal pin with a ball pen and turn wheel carefully. This pin
overcomes the automatic clutch mechanism. Thus, care must be taken not to apply excessive
pressure.
42
Instruction Manual TPersonal 10/2009
Lid arrested in upper position:
carefully turn wheel with pressed pin clockwise, until you feel
normal resistance (no more clicking noise, clutch is released).
Release pin and turn lid down, until the clutch mechanism is
activated (clicking noise, optimum pressure applied).
Lid arrested in down position:
carefully turn wheel with pressed pin counter clockwise, until you
feel normal resistance (no more clicking noise, clutch is released).
Release pin and turn wheel counter clockwise until pressure is
completely released. Open lid.
Important: When the clutch mechanism is active (= optimum pressure is applied), Do not use
pin to further increase pressure. This may lead to damage of tubes and instrument!
Instruction Manual TPersonal 10/2009
43
8
Maintenance and repair
8.1 Cleaning and Maintenance
The TPersonal was built to operate for a long time without the need for periodical maintenance.
Nevertheless, occasionally cleaning of the air inlet may be necessary to maintain the efficiency
of the Thermocycler. Insufficient airflow may lead to reduced heating and cooling rates (see
chapter 7.1). The inlet for the airflow is located at the bottom side of the instrument. Be sure that
the inlet is not clogged by dust or other material (e.g. a sheet of paper placed under the cycler
can be attached to the inlet as the fan is in operation). Dust can be removed easily from the inlet
with a conventional vacuum cleaner. Additionally, the Thermocycler housing may be cleaned
from time to time with a smooth cotton cloth. Do not use strong detergents or organic solvents
for cleaning. Never treat silver block with abrasive agents.
Important: Appropriate safety regulations must be observed when working with infectious or
pathogenic material.
8.2 Servicing and repair
The TPersonal Thermocycler contains no user serviceable parts. Do not open the housing
instrument. Service and repair may only be carried out by the Biometra Service department or
otherwise qualified technical personal.
The Service department offers Thermocycler maintenances and temperature verifications.
Biometra recommends an annual maintenance and a biannual temperature check for all
Thermocyclers. Please call the following phone number for detailed information: +49 55150881-10/12.
8.3 Firmware update
For instruction for firmware upgrade, please contact the Biometra Service Department or your
local distributor/sales representative.
8.4 Replacement of Spare Parts
Only original spare parts mentioned in these operating instructions are allowed.
44
Instruction Manual TPersonal 10/2009
9
9.1
Accessories
Plasticware
Biometra offers a broad range of plasticware for the use in PCR. The following table provides an
overview for the product portfolio:
Cat. no.
050-310
050-320
050-254
050-255
050-225
Description
Single tubes
0.2 ml tubes with caps
0.5 ml tubes with caps
Tube strips
Strips 8 tubes and flat caps
Strips 8 tubes and domed
caps
48 well plates
48 well microplate
050-253
96 well plates
96 well skirted
96 well non-skirted (low
profile)
96 well non-skirted
050-231
050-240
384 well plates
HSQ 384 well skirted
384 well microplate
050-232
050-213
050-237
050-256
050-257
050-236
050-194
050-694
050-251
050-252
Instruction Manual TPersonal 10/2009
Miscellaneous
Silcone mat
Adhesive film
Heat Sealing Film
Heat Sealing Film
Aluminum
96 well aluminium plate
384 well aluminium plate
Nop mat 96 well
Nop mat 384 well
Quantity
1000 pcs.
1000 pcs.
125 pcs.
125 pcs.
50 pcs.
25 pcs.
25 pcs.
25 pcs.
50 pcs.
50 pcs.
50 pcs.
100 pcs.
100 pcs.
10 pcs.
1 pcs.
1 pcs.
20 pcs.
20 pcs.
45
10 Service
Should you have any problems with this unit, please contact our service department or your
local Biometra dealer:
Biometra GmbH
Service Department
Rudolf-Wissell-Straße 14 - 16
D-37079 Göttingen
Phone:++49 (0)5 51 50 68 6 - 10 or 12
Fax: ++49 (0)5 51 50 68 6 -11
e-mail: [email protected]
If you would like to send the unit back to us, please read the following return
instructions in chapter 10.1.
10.1 Instructions for return shipment
In case of an instrument failure that cannot be fixed by the procedures described in section 7
please proceed as follows:
•
•
•
•
Return only defective devices. For technical problems which are not definitively recognisable
as device faults please contact the Technical Service Department at Biometra (Tel.: +49 55150881-10/12, Fax: +49 551-50881-11, e-mail: [email protected]).
Please contact our service department for providing a return authorization number (RAN).
This number has to be applied clearly visible to the outer box. Returns without the RAN will
be not be accepted!
Important: Carefully clean all parts of the instrument of biologically dangerous, chemical or
radioactive contaminants. If an instrument is contaminated, Biometra will be forced to refuse
to accept the device. The sender of the repair order will be held liable for possible losses
resulting from insufficient decontamination of the device.
Please prepare written confirmation that the device is free from biologically dangerous and
radioactive contaminants. The declaration of decontamination (see section 11) must be
attached to the outside of the packaging.
•
Use the original packing material. If not available, contact Biometra or your local distributor.
•
Label the outside of the box with “CAUTION! SENSITIVE ELECTRONIC INSTRUMENT!”
•
Please enclose a note which contains the following:
46
a)
Sender’s name and address,
b)
Name of a contact person for further inquiries with telephone number,
c)
Description of the fault, which also reveals during which procedures the fault occurred, if
possible
Instruction Manual TPersonal 10/2009
10.2 Packing of the Thermocycler
Biometra uses an extra designed packaging system where the instrument is mounted in
between two tearproof foils. The Thermocycler is put onto the lower inlet and is fixed in between
the foils by pressing the upper inlet down.
Note: The Thermocycler is only protected from transport damage if the packing instructions are
followed and the instrument is mounted in between the foils. Biometra will not be responsible for
transport damage by improper packing.
Gerät zwischen den
Folien schwebend
einspannen!
Mount instrument
floating in between
the foils!
Instruction Manual TPersonal 10/2009
47
11 Equipment Decontamination Certificate
To enable us to comply with german law (i.e. §71 StrlSchV, §17 GefStoffV and §19 ChemG)
and to avoid exposure to hazardous materials during handling or repair, please complete this
form, prior to the equipment leaving your laboratory.
COMPANY / INSTITUTE
__________________________________________________
ADDRESS
______________________________________________________________
PHONE NO
_________________________
E-MAIL
______________________________________________________________
EQUIPMENT
FAX NO_________________________
Model
______________
Serial No
______________
______________
______________
______________
______________
______________
______________
If on loan / evaluation Start Date: __________________
Finish Date __________________
Hazardous materials used with this equipment:
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
Method of cleaning / decontamination:
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
The equipment has been cleaned and decontaminated:
NAME __________________________________ POSITION _______________________
(HEAD OF DIV./ DEP./ INSTITUTE / COMPANY)
SIGNED ________________________________ DATE ____________________________
PLEASE RETURN THIS FORM TO BIOMETRA GMBH OR YOUR LOCAL BIOMETRA
DISTRIBUTOR TOGETHER WITH THE EQUIPMENT.
PLEASE ATTACH THIS CERTIFICATE OUTSIDE THE PACKAGING. INSTRUMENTS
WITHOUT THIS CERTIFICATE ATTACHED WILL BE RETURNED TO SENDER.
48
Instruction Manual TPersonal 10/2009
General Information for Decontamination:
Please contact your responsible health & safety officer for details.
Use of radioactive substances:
Please contact your responsible person for details.
Use of genetically change organism or parts of those:
Please contact your responsible person for details.
Instruction Manual TPersonal 10/2009
49
12 Note for the disposal of electric / electronic waste.
Note
for disposal of electric / electronic waste
Hinweis
für die Entsorgung von Elektroaltgeräten
Renseignement
du traitement des déchets des appareils
électrique / électronique
This symbol (the crossed-out wheelie bin) means, that this product should be brought to the return and / or
separate systems available to end-users according to yours country regulations, when this product has
reached the end of its lifetime.
For details, please contact your local distributor!
This symbol applies only to the countries within the EEA*.
EEA = European Economics Area, comprising all EU-members plus Norway, Iceland and Liechtenstein.
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Dieses Symbol (die durchgestrichene Abfalltonne) bedeutet, dass dieses Produkt von der Firma Biometra für
eine kostenlose Entsorgung zurückgenommen wird. Dies gilt nur für Geräte, die innerhalb Deutschlands
gekauft worden sind.
Kontaktieren Sie für die Entsorgung bitte die Biometra Service-Abteilung!
Außerhalb Deutschlands wenden Sie sich bitte an den lokalen Händler.
Dieses Symbol gilt nur in Staaten des EWR*.
*EWR = Europäischer Wirtschaftsraum, umfasst die EU-Mitgliedsstaaten sowie Norwegen, Island und
Liechtenstein.
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Cet symbol (conteneur à déchets barré d´une croix) signifie que le produit, en fin de vie, doit être retourné à
un des systèmes de collecte mis à la disposition des utilisateurs finaux en conséquence des régulations par
la loi de votre pays.
Pour des information additionel nous Vous demandons de contacter votre distributeur!
Cet symbole s´ápplique uniquement aux pays de l´EEE*.
EEE = Espace économique européen, qui regroupe les États membres de l´UE et la Norvège, Islande et le
Liechtenstein.
50
Instruction Manual TPersonal 10/2009
13 Declaration of Conformity
Göttingen, den 20. 08. 2004
im Sinne der EG-Richtlinie über elektrische Betriebsmittel zur Verwendung innerhalb
bestimmter Spannungsgrenzen 73/23/EWG
following the EC directive about electrical equipment for use within certain limits of voltage
73/23/EEC
und / and
im Sinne der EG-Richtlinie für die elektromagnetische Verträglichkeit 89/336/EWG.
following the EC directive about the electromagnetic compatibility 89/336/EEC .
Hiermit erklären wir, daß folgende Thermocycler:
Herewith we declare that the following Thermocyclers:
Typen / types:
Best.-Nr. / Order No.:
TPersonal 20, TPersonal 48,
TPersonal combi
050-720, 050-723, 050-724
den grundlegenden Anforderungen der
corresponds to the basic requirements of
EG-Niederspannungsrichtlinie 73/23/EWG und der
EC low voltage directive 73/23/EEC and the
EG-Richtlinie über die elektromagnetische Verträglichkeit 89/336/EWG entsprechen.
EC directive about the electromagnetic compatibility 89/336/EEC .
Folgende harmonisierte Normen wurden angewandt:
The following harmonized standards have been used:
EN 55011:1998 + A1:1999 + A2:2000
EN 61000-3-2:2000
EN 61000-6-1:2001
EN 61010-1: 2001
EN 55022:1998 + A1:2000 + A2:2003
EN 61000-3-3:1995 + A1:2001
EN 61010-2-010: 1994 + A1: 1996
Dr. Jürgen Otte
Quality Manager
Instruction Manual TPersonal 10/2009
51
14 Warranty
This Biometra instrument has been carefully build, inspected and quality controlled before
dispatch. Hereby Biometra warrants that this instrument conforms to the specifications given in
this manual. This warranty covers defects in materials or workmanship as described under the
following conditions:
This warranty is valid for 24 month from date of shipment to the customer from Biometra. This
warranty will not be extended to a third party without a written agreement of Biometra.
This warranty covers only the instrument and all original accessories delivered with the
instrument. This warranty is valid only if the instrument is operated as described in the manual.
Biometra will repair or replace each part which is returned and found to be defective. This
warranty does not apply to wear from normal use, failure to follow operating instructions,
negligence or to parts altered or abused.
52
Instruction Manual TPersonal 10/2009
15 Subject Index
Beep
on/off .................................................... 36
Contrast.................................................... 36
Control panel ............................................ 17
Declaration of Conformity......................... 51
Decontamination Certificate ..................... 48
Directory
select .................................................... 19
Display...................................................... 19
Disposal.................................................... 50
Firmware update ...................................... 44
Heated lid ................................................. 18
blockade ............................................... 42
pre-heating ........................................... 22
Language ................................................. 36
Maintenance............................................. 44
Operation Voltage .................................... 13
Packing..................................................... 47
Plasticware ............................................... 45
Program
copy...................................................... 28
create ................................................... 23
delete.................................................... 30
delete step............................................ 26
Instruction Manual TPersonal 10/2009
edit ....................................................... 26
heating and cooling rates ..................... 32
insert step............................................. 27
lid temperature ..................................... 22
loop ...................................................... 24
name .................................................... 21
pause ................................................... 34
save...................................................... 25
start ................................................ 32, 33
stop ...................................................... 34
time increment...................................... 31
touch down........................................... 32
Remaining run time .................................. 34
Repair ...................................................... 44
Safety precautions ..................................... 9
Self test .................................................... 14
Servicing .................................................. 44
Short manual............................................ 38
Subject Index ........................................... 53
System info .............................................. 35
System settings........................................ 35
Technical specifications ............................. 7
Trouble shooting ...................................... 42
Warranty .................................................. 52
53
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