"user manual"

"user manual"
THERMAL CYCLER
TC-PRO
Instruction Manual
Note:
The Boeckel + Co (GmbH + Co) KG reserves the right to
modify this manual at any time without notice.
Patent materials are included in this manual. All rights reserved. Any part of
this manual shall be forbidden to photocopy, reproduce, or translate into other
languages, without written permission from the Boeckel + Co (GmbH + Co)
KG
Please read this manual carefully before the Thermal Cycler is first operated!
Document Number: BYQ60580100000RSM
Document Version: Mar, 2008, Version 1.0
BOECO TC-PRO Thermal Cycler
Important Note
1
CONVENTIONS
Note:
Because there is important information in this item, please read it carefully.
Failure to follow the advice here will possibly result in damage or malfunctioning
of the Thermal Cycler.
Warning! This symbol means that you should be cautious to take an operation/procedure.
Failure to follow the requirements in this item will possibly result in personal
injury.
During the operation, maintenance, or repair of the BOECO TC-PRO Thermal Cycler, the
following safety measures should be taken. Otherwise, the guard provided by the Thermal
Cycler is likely to be damaged, while the rated safety level to be reduced, and the rated
operation conditions to be affected.
The Boeckel + Co (GmbH + Co) KG shall not be in any way responsible for the
consequences resulted from operators not following the requirements
Note:
The Thermal Cycler, complying with the National Standard GB4793.1, is a
general instrument of class Ⅱ, the protection degree is IP20. It is intended for
indoor use.
a) Grounding Considerations
A.C. power’s grounding should be grounded reliably for fear of an electric shock. The 3-pin
plug with the Thermal Cycler’s power line is a safety device that should be matched with a
grounded socket. Never let the third ground pin floating. If the 3-pin plug cannot be inserted,
it is recommended to ask an electrician to install an appropriate power socket.
b) Keep Away from Electric Circuits
The operator is not allowed to open the Thermal Cycler. To changing components or adjust
certain parameters inside the device must only be accomplished by the certificated
professional maintenance personnel. Do not change elements while the power is still on.
c) A.C. Power Considerations
Before turn on the power, always check if the mains voltage is within the required A.C.(±10%
difference is allowed.) and whether the current rating of the power socket meet the required
specification.
d) A.C. Power Line Considerations
As an accessory of the Thermal Cycler, the A.C. power line used should be a default one. If
1
BOECO TC-PRO Thermal Cycler
it is damaged, the A.C. power line may not be repaired, but could be replaced by a new one
with same model and specification. The power line should be free of heavy objects during
the Thermal Cycler’s operation. Keep the power line away from the place where people
gather regularly.
e) Connect the A.C. Power Line
While connect or disconnect the power line, you should hold the 3-pin plug with your hand.
Insert the plug thoroughly to ensure good contact between the plug and socket. Pull the plug,
but not the line, when you need to disconnect to the mains.
f) Requested Environments
The Thermal Cycler should be placed in a low-humidity, dust-free, and good-ventilation room
without caustic gas or powerful magnetic interference. In addition, water sources, such as
pools and water pipes, should be kept a distance from the Thermal Cycler.
Never cover or obstruct the openings of the Thermal Cycler, which are designed for
ventilation and prevent the device’s interior from being too hot. When a single device is
running, the shortest distance between its openings and the nearest object is 50cm; when
two or more devices are running at the same time, the shortest distance is 100 cm. Do not
place the device on a soft surface, for that will result in adverse ventilation near the device’s
bottom openings.
Too high temperature will lead to degraded performance or failure of the Thermal Cycler.
Therefore, the device should be protected against any kind of heat sources like sunlight,
ovens, or central heating equipment.
If the Thermal Cycler is set aside for a long time, it is recommended to disconnect the power
line to mains and cover the device with a piece of soft cloth or plastic to prevent dust from
entering.
Note:
Once one of the following events occurs, you are suggested to disconnect the
power line with mains, and contact the distributor or ask a certificated
maintenance personnel for help.
 Liquid into the device;
 The device sprinkled or drenched;
 The device malfunctioning, giving off abnormal sound or odor;
 The device falling onto the floor or its shell damaged;
 Significant changes in the device’s performance.
2
BOECO TC-PRO Thermal Cycler
3
LABEL
a) Tablet
Name: Thermal Cycler
Model: TC-PRO
Power: ~100-240V
50/60Hz
600W
Name: BLOCK 96G
Model: B-96G
SN: *******-***
Date:****.**
Fuse: 250V 8A Φ5×20
Sort: class Ⅱ
IP20
SN: *******-***
Date: ****.**
Boeckel + Co(GmbH+ Co)KG
Hamburg,Germany
b) Warning Sign
Warning!
4
There are two warning signs read ‘HOT SURFACE!’ During the program
execution and in the short period after the program execution, the metal part
near those signs (on the block and hot lid) is not allowed to be touched by
any part of the body for fear of the body burn!
MAINTENANCE
The conical holes over the block should be cleaned regularly with soaked cotton swab in
order to ensure sufficient contact and thus good heat conduction between each conical hole
and the tube inside it.
3
BOECO TC-PRO Thermal Cycler
Warning!
5
When cleaning, the instrument should be off.
Corrosive scour is not allowed to clean the surface of the instrument.
WARRANTY AND SERVICE INFORMATION
Please refer to the Warranty Card for the detailed information.
Note:
Once it is opened, the package should be checked according to the packing
list. If the buyer finds any items to be missing or damaged, do not hesitate to
contact the distributor.
After the acceptance check is passed, the buyer should fill out the check form
and send its photocopy (or fax it) to the distributor. The Boeckel + Co (GmbH +
Co) KG establishes the archives and maintenance records with the returned
form.
Please store the package and packing materials in a safe place in case of
future device maintenance. The above warranty does not extend to goods
damaged as the result of cheesy package.
4
BOECO TC-PRO Thermal Cycler
Contents
Contents
Chapter One Introduction........................................................................................................... 1
1. Brief Introduction of PCR Technique ..................................................................................... 1
2. PCR Applications ................................................................................................................... 1
3. Features of BOECO TC-PRO ................................................................................................ 2
4. Operations of BOECO TC-PRO ............................................................................................ 3
Chapter Two Specifications ....................................................................................................... 5
1. Normal Working Conditions ................................................................................................... 5
2. Conditions for Transportation and Storage............................................................................ 5
3. Basic Parameters................................................................................................................... 5
4. Performances ......................................................................................................................... 6
5. Software Functions ................................................................................................................ 6
Chapter Three Preparations ...................................................................................................... 8
1. Structure Description ............................................................................................................. 8
2. Keyboard Diagram ................................................................................................................. 9
3. Keyboard Instructions ............................................................................................................ 9
4. Installation of Block Unit....................................................................................................... 10
4.1 Installation steps ......................................................................................................... 10
4.2 Dismantlement Steps ................................................................................................. 11
5. Inspections before Power-on ............................................................................................... 12
6. Power-on .............................................................................................................................. 12
Chapter Four Operation Guide ................................................................................................ 13
1. Block..................................................................................................................................... 13
2. How to run a single-block PCR program ............................................................................. 14
2.1. How to edit PCR programs........................................................................................ 16
2.2 How to set system parameters ................................................................................... 20
3. How to run a double- block PCR program........................................................................... 22
3.1 How to edit a PCR program........................................................................................ 23
3.2 How to set system parameters ................................................................................... 26
4. How to run a gradient block PCR program.......................................................................... 27
BOECO TC-PRO Thermal Cycler
Contents
4.1 How to edit a PCR program........................................................................................ 28
4.2 How to set system parameters ................................................................................... 31
4.3 Characteristics of Temperature Distributing of Gradient Block.................................. 31
5 How to Input and Output the PCR Program ......................................................................... 32
5.1 Connect Usb Flash Memory ....................................................................................... 32
5.2 Input file from usb flash memory ................................................................................ 33
5.3 Export file to usb flash memory .................................................................................. 34
Chapter Five Connection of Computer .................................................................................... 36
1. Software installation ............................................................................................................. 36
2. Online setup ......................................................................................................................... 36
3. Software installation ............................................................................................................. 36
4. Configure your equipment.................................................................................................... 38
4.1 Network Connection ................................................................................................... 38
4.2 Network Enable Adminastrator................................................................................... 39
4.3 Configure .................................................................................................................... 40
5. Main interface....................................................................................................................... 44
6. Toolbar ................................................................................................................................. 45
7. Basic manipulation ............................................................................................................... 45
8. User manual ......................................................................................................................... 48
9. File menu ............................................................................................................................. 50
10. Tool menu .......................................................................................................................... 55
Chapter Six Firmware Upgrade ............................................................................................... 58
1.Configure your equipment..................................................................................................... 58
2. How to Upgrade the Software.............................................................................................. 58
Chapter Seven
Failure Analysis and Troubleshooting.......................................................... 66
1. Failure Analysis and Troubleshooting.................................................................................. 66
2. Spare Parts list..................................................................................................................... 68
Appendix 1
Wiring Diagram................................................................................................... 69
Appendix 2
Parameter Value & Meaning ............................................................................ 70
BOECO TC-PRO Thermal Cycler
Chapter One Introduction
Chapter One Introduction
This chapter briefly introduces PCR technique and its applications, PCR thermal cyclers,
and the characteristics of BOECO TC-PRO Thermal Cycler.
1. Brief Introduction of PCR Technique
PCR (Polymerase Chain Reaction), or cell-free molecule cloning, is a technique for
amplifying nucleic acids in vitro, emulating natural DNA replication process. The PCR
technique, using two DNA strands to be amplified as template, and a pair of synthetic
oligonucleotides as primers, rapidly reproduces the specific DNA pieces under the catalysis
of a thermostable DNA polymerase. Because of its simplicity, rapidity, specificity, and
sensitivity, PCR has been widely applied to all fields of life sciences since its invention by
Mullis in 1983 and the discovery of the thermostable DNA polymerase by Erlich in 1989.
Great achievements have been obtained in PCR’s applications in such areas as cytology,
virology, oncology, genetics, forensics, and immunology. PCR technology is a milestone in
the history of modern molecular biology.
2. PCR Applications
Research Areas
Gene cloning, DNA sequencing, mutation analysis, gene recombination
and mutagenesis, identification and adjustment of DNA sequence of
protein structure, detection of gene extension, construction of synthetic
genes, construction of cloning and expression carrier, detection of
polymorphism of a gene’s inscribed enzyme;
Clinical
Diagnoses
Bacteria (spirochaeta, rickettsia, diphtheria bacillus, colon bacillus,
dysentery bacillus, and clostridium); Virus (HTLV, HIV, HBV, HCV,
HPVS, EV, CMV, EBV, HSV, measles virus, rotiform virus, B19 virus,
and Lhasa virus); Parasite (malaria); Human hereditary diseases
(Lesh-Nyhan syndrome, hemophilia, BMD, and DMD);
Immunology
HLA locus typing, qualitative analysis of T-cell receptor or antibody
diversification, immune body gene mapping, quantitative analysis of
lymph genes;
Human Genome
Project
Identification of DNA markers using discrete repetitive;Sequence,
construction of a genetic linkage map (detection of DNA, polymorphism,
or semen mapping); Construction of a physical map, sequencing, and
map expression;
— 1 —
BOECO TC-PRO Thermal Cycler
Chapter One Introduction
Forensics
Specimen analysis in the venue, and HLA-DQα locus typing;
Oncology
Pancreas cancer, rectum cancer, lung cancer, thyroid gland cancer,
melanin cancer, and leukemia;
Social and
Colony Biology
Genetic species research, evolution research, animal protection
research, ecology, environmental sciences, and experimental genetics;
Paleontology
Specimen analysis in archeology and museum;
Biology
Diagnosis of animal hereditary diseases and detection of plant
pathogeny.
3. Features of BOECO TC-PRO
BOECO TC-PRO Thermal Cycler is a PCR detection system with multiple functions. It has
features and characteristics as followings:

Provide five kinds of interchangeable block units to meet various PCR experiments’
requirements;

Adopting American ITI advanced thermoelectric cooling technology and thermoelectric
cooling components manufactured under Japanese quality controlling mode, BOECO
TC-PRO Thermal Cycler possess the stable and reliable performance;

With speedy temperature ramping rate at the maximum of 4 /S, BOECO TC-PRO can
adjust ramping rate according to different experimental requirements;

With multiple temperature-control modes,
the
instrument has much stronger
compatibility;

The central processor of BOECO TC-PRO Thermal Cycler can automatically distinguish
block units, as well as run other relevant programs;

With colourful extra large screen LCD and human-oriented operating interface, its
operation becomes simple and clear

It has the function of auto power off protection, and continues to complete the
unfinished program after the power supply is resumed;

Height and pressure of hot lid is adjustable so as to fit different reacting tubes on the
basis of practical operating situations, in order to prevent reagents from the evaporation
and pollution;

RJ45 standard interface can be connected with normal computers, thus to achieve easy
software upgrading.
— 2 —
BOECO TC-PRO Thermal Cycler
Chapter One Introduction
4. Operations of BOECO TC-PRO
Adopting many advanced techniques, blocks of BOECO TC-PRO Thermal Cycler can meet
different PCR experiments’’ requirements. It holds a leading position in the field of similar
products.
BOECO TC-PRO Thermal Cycler Adaptation Carrier:
BLOCK 96G:
Standard
96×0.2ml (Gradient block)
Centrifuge
Tube 0.2ml
+
BLOCK 3048: 30×0.5ml+48×0.2ml
Standard Centrifuge Tube 0.2ml
+ Standard Centrifuge Tube 0.5ml
BLOCK 384G: 384-well plate (Gradient block)
384-well plate
+
BLOCK 48D: 48×0.2ml+48×0.2ml
— 3 —
BOECO TC-PRO Thermal Cycler
Chapter One Introduction
Standard Centrifuge Tube 0.2ml×2
BLOCK 4I: 4pcs. In Situ plate
In Situ plate
— 4 —
BOECO TC-PRO Thermal Cycler
Chapter Two Specifications
Chapter Two Specifications
This chapter describes the Thermal Cycler’s operation, transportation and storage conditions,
basic parameters, performance and functions.
1. Normal Working Conditions
Ambient Temp.: 10℃ ~ 30℃
Relative Humidity: ≤70%
Power Supply : ~100-240V 600W 50/60Hz
Note:
Before power-on, please check whether the above working conditions are
satisfied. Pay special attention to the power line’s reliable grounding.
2. Conditions for Transportation and Storage
Ambient Temp.: -20℃ ~ +55℃
Relative Humidity: ≤ 80%
3. Basic Parameters
Model
Model of
Main Body
Model of
Block
Sample
Capacity
Temp.
Range
Heating
Rate(max)
Cooling
Rate(max)
TC-PRO-96G
TC- PRO -3048
TC- PRO
-384G
TC- PRO -48D
TCPRO -4I
TC-E
B-96G
B-3048
B-384G
B-48D
B-4I
96×0.2ml
96micro plate
12x8 strip
tube
8x12strip
tube
48×0.2ml+30×0.5
ml
8 Strip tube
384-well Plate
48×0.2ml+48×0.2
ml
4pcs. of
In Situ
plate
4℃ ∼ 99℃
≥4.0℃
≥2.8℃
≥2.8℃
≥4.0℃
≥1.8℃
≥4.0℃
≥2.8℃
≥2.8℃
≥4.0℃
≥1.8℃
— 5 —
BOECO TC-PRO Thermal Cycler
Chapter Two Specifications
≤±0.4℃(95℃)
Temp.
Uniformity
≤±0.3℃(72℃)
≤±0.3℃(55℃)
Temp.
Accuracy
Max.
Gradient
Min.
Gradient
Set temp.
range of
hotlid
≤±0.3℃
30℃
30℃
╱
1℃
╱
1
30
∼ 110
Dimension
470mm×340mm×260mm(L×W×H)
Weight(kg)
10
Fuse
250V
PC Interface
Note:
╱
8A
Ф5×20
RJ45
When temperature set value of hot-lid is lower than that of block, the
temperature of hot-lid showed in the display would be higher than the set
value.
4. Performances
Maximum number of cycles:99
Maximum number of sections: 5
Maximum procedures within a cycle:16
Number of programs:99
Noise:59dB (Background Noise:below 40dB testing on 1m dead ahead)
5. Software Functions
File editing and saving
File accessing, modifying and deleting
Adjusting the heating/cooling rate automatically
Automatic temperature &time increments/decrements during cycling program execution
Displaying the data at each phase of program execution instantly
— 6 —
BOECO TC-PRO Thermal Cycler
Chapter Two Specifications
Pause of program running
Stop of program running
Auto-restart in case of power failure
Soft upgrading via RJ45 interface
Sound alarm
Estimating total program execution time and remaining program running time
Date and time (year, month, day, hour, minute, second) display and calibration
Failure protection and alarming
Note:
The above software functions are for reference only. The Boeckel + Co
(GmbH + Co) KG reserves the right to modify the software functions without
notice.
— 7 —
BOECO TC-PRO Thermal Cycler
Chapter Three Preparations
Chapter Three Preparations
This chapter introduces the BOECO TC-PRO Thermal Cycler’s mechanical structure, the
keyboard and each button’s functions, and some preparations before power-on. Before any
operation, please read this chapter carefully and make sure you are familiar with the steps
introduced.
1. Structure Description
①
④
②
⑤
⑥
③
⑦
⑩
⑧
⑾
⑨
①
④
⑦
⑩
Block
Keyboard
Sticker of Main body
Sticker of Block
②
⑤
⑧
⑪
LCD Display
Ventilating Hole
RJ45 interface
Power Socket
— 8 —
③
⑥
⑨
⑫
Logo
USB interface
Switch
Fuse Socket
BOECO TC-PRO Thermal Cycler
Notes:
Chapter Three Preparations
The fuse specifications are as follows: 250V 8A, Φ5×20mm. The fuse should be
replaced by one that meets those specifications. If you need certificated fuses,
please contact the distributor or manufacturer.
2. Keyboard Diagram
3. Keyboard Instructions
“Digit & Letter”, select
the letter by pressing in
succession.
— 9 —
BOECO TC-PRO Thermal Cycler
Chapter Three Preparations
“Shift”,shift from one block to another
“Function”,change “+, -” when editing process temperature
and modifying time
“Right/Left”,moving the cursor one position to the left or right
when pressing during file parameter setting.
“Up/Down”,moving the cursor one position to the up or down
when pressing during file parameter setting.
“Enter” ,press it to accept the present parameters setting
displayed on the screen when setting documents.
“Menu”, press any button to accept the current items displayed
above the button..
4. Installation of Block Unit
The main unit must link with the block unit so as to work normally. Please install or dismantle the
block unit according to the following steps:
4.1 Installation steps
a)
Put the block onto the slot of main body, uplift the locking handle, and push the block slowly
until the locking handle is in the right place.
b)
Press the locking handle downwards, thus the block will move forwards automatically. The
sound “kaka” means that the block is installed in the right place.
c)
Power-on and the instrument can run normally.
— 10 —
BOECO TC-PRO Thermal Cycler
①
②
Chapter Three Preparations
③
④
⑤
⑥
⑦
Main Body
Main Unit Connecting Plug
Locating set
Unlocking Device
Rotator
Locking Handle
Note:
1.
2.
3.
4.
Block
Make sure to switch off the power supply before installing block.
When install block, please pay close attention to push the block slowly;
Otherwise , the main unit connecting plug may be damaged.
When uninstall block, please draw the block from the slot gently. It’s forbidden
to lean the block for fear of snap of connection wire.
After installation, the block unit will be recognized by the main body
automatically, and will be ready for work without any other operation.
4.2 Dismantlement Steps
a)
Power off firstly, make sure to dismantle or replace the block.
b)
Uplift the locking handle, it will sound “kaka”, and the block will move backwards
simultaneously.
c) Take out the block through the locking handle. The dismantlement is finished.
— 11 —
BOECO TC-PRO Thermal Cycler
Chapter Three Preparations
5. Inspections before Power-on
Before switch the power on, please ensure that:
1) Supply voltage falls within the specified limits (refer to Chapter Two);
2) The plug has been inserted into the power socket reliably;
3) The grounding of the power line is reliable.
6. Power-on
Turn on the power (i.e. turn the power switch to position “–“), the BOECO TC-PRO Thermal
Cycler will beep twice, and the fan start to running, which means the instrument is power-on.
At this time, he LCD will display “Self testing ••••••”, which means the instrument is under
self-testing. It will last for about 1-2 minutes. Please wait patiently. After the self-testing, the main
menu will be displayed, and the instrument is ready for operations such as editing, accessing,
modifying, or deleting a PCR amplification file.
— 12 —
BOECO TC-PRO Thermal Cycler
Chapter Four Operation Guide
Chapter Four Operation Guide
There are five kinds of block units suitable to BOECO TC-PRO Thermal Cycler. In this chapter,
we will explain in details on operations of every kind of block unit, for example, how to edit,
access, modify, delete, or run a PCR amplification file, how to set parameters under different
block unit.
Warning!
Please turn off the power immediately and contact the distributor if abnormal
sound or display appears after power-on, or if failure alarm and display are found
in the process of the device’s self-testing.
Note:
If the number of samples is less than the number of conical holes of the block,
the sample tubes should be evenly distributed over the block, in order to ensure
that the heated lid will stably press on the top of the sample tubes, so that both
the block’s load and the temperature distribution in each tube are even.
Note:
Before closing the cover, please revolve the knob counter-clockwise according to
the knob diagram till there sound "dang, dang....." Please note that the knob can
be tightened by revolving clockwise, but can be loosed by revolving
counter-clockwise.
After closing the cover but before power on, please revolve the knob clockwise
till it sound "dang,dang....." Revolve the knob one more turn.
At the beginning of revolving the knob clockwise or counter-clockwise, you can
hear "dang,dang...." sound, press the unlocking device by your finger;
meanwhile, revolve the knob counter-clockwise for two turns, thus to loose the
unlocking device to revolve the knob.
1. Block
BOECO TC-PRO has five models with different purpose. And each model has its own running
program.
B96G
Gradient block program
B3048
Mixed block program
— 13 —
B384G
Gradient block program
BOECO TC-PRO Thermal Cycler
Chapter Four Operation Guide
B48D
B4I
Dual-block program
Single-block program
2. How to run a single-block PCR program
Switch the power on, BOECO TC-PRO Thermal Cycler will beep twice, and it means the power
supply is connected well. The screen will display “Self testing ••••••”,and the instrument will
carry on self testing. The self-testing needs about 1~ 2 minutes and please wait patiently. If the
self-testing is successful, the screen will display the main interface.
Main Interface
On the left top of the main interface it
displays the state of Hot lid.
Hotlid=off
2007-11-1 10:05:00
On the central area it displays Boeckel +
Co (GmbH + Co) KG information, software
BOECO
version number, block model, default file,
default user, control mode and sample
volume.

Boeckel + Co (GmbH + Co) KG
Version: 2007-1.0
Block:
**×**
Default User: BOECO
Default File: PCR1
Control Mode: Block
Press “File” to enter into file list
interface.

Press “System” to enter into system
parameter setting interface.

If the current file doesn’t have code,
press “Run” to enter into running
interface
and
confirmation;If
the
tube
current
mode
Control
File
Run
Mode has code, press “Run”, thus the screen shows the following interface.
— 14 —
System
BOECO TC-PRO Thermal Cycler
Chapter Four Operation Guide
Main Interface
In this interface, press “Number” key to
input code.

Press “Back” to go back to the main
Hot lid=off
2007-11-1
10:05:00
BOECO
interface.

Press ”Accept” key to confirm the code.

If the module is mixed block, press
Boeckel + Co (GmbH + Co) KG
Version: 2007-1.0
Block:
**×**
Default User: BOECO
Default File: PCR1
Control Mode: Tube
Input password
"Accept" to determine the test-tube type;
pop-up tube type selection dialog.

If the module is not mixed block, the
current Control Mode is Block mode
and press "Accept" running into the file
operation
interface;
If
the
current
control mode is Tube mode, press
Back
"Accept" key, then pop-up tube vol. Settings dialog box.
Accept
Tube style selection
In this interface press +/- can choice the
Hotlid=off
actual tube type.

Press "Back" key to go back to the
BOECO
main interface.

Press "next". If the current control
Boeckel + Co (GmbH + Co) KG
Version: 2007-1.0
Block:
**×**
Default User: BOECO
Default File: PCR1
Control Mode: Tube
mode is Block Mode, click "next" to
enter document operation interface; if
the current mode is Tube Mode, press
“next” key pops up the
2007-11-1 10:05:00
following
interface.
Select Tube Type
0.2ml
050μl
Usblink
— 15 —
Back
next
BOECO TC-PRO Thermal Cycler
Chapter Four Operation Guide
In this interface, press “Character” key to input
number of sample volume (Unit: Micro liter)
Hotlid=off
BOECO
according to the practicality. Under Tube Mode,
samples with different concentrations have
different overshooting temperature and duration
Boeckel + Co (GmbH + Co) KG
Version: 2007-1.0
Block:
**×**
Default User: BOECO
Default File: PCR1
Control Mode: Tube
time.

2007-11-1 10:05:00
Press “Back” to go back to the main
interface.
 Press “Run” to enter into file running
interface.
Select Sample Volume:
050μl
050μl
Back
next
2.1. How to edit PCR programs
File List Interface
Press “File” on the main interface to enter file
list interface. There are 3 files listed in the form
as showed in the picture. When the flashing bar
is flashing in the User Column, select users
through pop-up key; at this time, the File Name
Hotlid=off
No.
User
1
BOECO1*
2
BOECO2
3
BOECO3
2007-11-1 10:05:00
File Name
Save Time
PCR1
2007-11-1
PCR2*
2007-11-1
PCR3
2007-11-1
Column will display the relative files of the
selected user dynamically. Shift to the File
Name Column, where the flashing bar will flash,
then select relative user’s file through pop-up
key.

Press “Edit” to edit the file indicated by the
cursor.
Edit
 Press “New File” to edit a new file.

New File
Delete
Back
Run
Press “Delete” and the system will alarm “Confirm delete file?”, then according to the alert to
select (Delete) or (BACK).

If the current file doesn't have code, press “Run” to enter into File Running Interface and tube
type confirmation; If the current file has code, press “Run”, thus there appears a dialog box.
Input Password:
-

If the module is mixed block, determine the test-tube type first; pop-up the following dialog
— 16 —
BOECO TC-PRO Thermal Cycler
Chapter Four Operation Guide
box.
Select Tube Type
0.2ml
050μl

If the current Control Mode is block mode, press "Run" running into the file operation
interface; If the current control mode is Tube mode, press "Run" key,then pop-up the
following dialog box.
Select Sample Volume:
050μl
The same as main interface, user could input the actual password, sample type and number of
sample volume (Unit: Micro liter) according to the practicality
Note:
The sign“*”at the top of User and File indicates the current running file is default
one, which can be modified in the parameter setting interface.
File Edit Interface
In this interface, you can edit sections and
segments of the file. A File is comprised
by sections and segments. And the
sections contain many segments, which
you can set cyclers to be run (refer to the
figure).
Move
cursor
by
pressing
“Up/Down” and “Right /Left”, and the
position that the cursor reaches will be
displayed in white on a black background.
Press
“Digit&Letter”
to
modify
parameter settings. Press “Function” to
Press “+Seg.” to enter
100
75
50
25
0
Temp
Time
Seg.
1c
2c
3c
4
into segment
2007-11-1 10:05:00
1c 95.0 2c 55.0
01:00
02:00
Temp(C)
95.0
55.0
72.0
04.0
Cycle1:×10
change +/-.

Hotlid=off
+Seg.
3c 72.0
01:45
Time
01:00
02:00
01:45
00:00
4 04.0
00:00
Ramp(C/s)
#.#
#.#
#.#
#.#
+Temp
+0.0
+0.0
+0.0
+0.0
+Time
+0:00
+0:00
+0:00
+0:00
From 01 to 03
+Cycle
Delete
Back
Save
edit interface. In every segment, the user can set setting temperature (Temp.), duration
(Time), Ramping rate (Ramp), temperature increment of every cycler(+Temp) and time
increment of every cycler (+Time). Press “Delete” to delete the present segment

Press “+Cycle” to enter into segment edit interface, where the user can set cycle numbers
and initial cycle segment. The figure shows a cycle from the first segment to the third one,
and there are totally 10 times cycles.

Press “+Cycle” repeatedly to add sections (max.: 5 sections ). Press “Delete” to delete the
current segment. Move cursor by pressing “Up/Down” to enter into the former segment or
— 17 —
BOECO TC-PRO Thermal Cycler
Chapter Four Operation Guide
the latter.

Press “Save” to enter into file save interface.

Press “Back” to go back to File List Interface.
Special Warning:
1. For the new edited PCR program, please save first and then run it, otherwise the file file
can’t be run;
2. When setting “Time” as “--:--”, the hot lid will be closed automatically in the case that
the block controls the temperature.
Relation of sections and segments in a
file
Seg. 1
Section 1
Cycle1 ×** From ** to **
Seg. 2
Section 2
File
Cycle2 ×** From ** to **
·
·
·
·
·
·
Section 5
Cycle5 ×** From ** to **
Seg. 16
— 18 —
BOECO TC-PRO Thermal Cycler
Chapter Four Operation Guide
File Save Interface
In this interface press “Digit&Letter” to
Hotlid=off
input the file name. Press “enter” to
2007-11-1 10:05:00
abc
move the cursor forwards one position.
Select User and File Name through
BIOR1
User:
pop-up key. Click left button to delete
characters while clicking right button to
File Name:
move the cursor. Press “Caps” key to
Password:
PC****
Password Check:PC****
shift the capital or small letter, and there
will be a capital or small letter indication
on the left top of the screen. (As abc
showed in the above picture)

PCR1
Main
Press “Main” to go back to the main
Caps
Save
Run
interface.

Press “Save” to save the file. When the file is normally saved, the system will remind “File
have been saved. Please continue another operation”. Press “Enter” key to cancel the
reminding bar. If the file was used, the system will remind you to rename (Rename) or
continue to save (Save).

Password setting press number memory.

If the module is mixed block, determine the test-tube type first; pop-up the following dialog
box.
Select Tube Type
0.2ml

If the current Control Mode is block mode, press "Run" running into the file operation
interface; If the current control mode is Tube mode, press "Run" key,then pop-up the
following dialog box.
Select Sample Volume:
050μl
The same as main interface, user could input the actual password, sample type and number of
sample volume (Unit: Micro liter) according to the practicality.
Note:
1 No difference between big and small letter, in setting file name and user name.
2 Please remenber the setting password.
3 Setting the password as 0~9 number character.
— 19 —
BOECO TC-PRO Thermal Cycler
Chapter Four Operation Guide
File Running Interface
During normal running of a file, it will be
flashing at the position of “Now Running•••”.
When the file is running over, the system will
alarm “File run over”.

Press “Stop” and it will remind “Confirm
stop running?”.

Please
select
“Stop”
or
“Start”
according to the reminding information.

Press “Pause” and it will remind “Now
pause
running?”
According
to
the
reminding information the user can
Hotlid=off
100
75
50
25
0
Temp
Time
2007-11-1 10:05:00
1c 95.0
2c 55.0
1:00
02:00
3c 72.0
4 04.0
01:45
00:00
File Name:PCR1
User: BOECO
Now Running •••
1c
Temp=00.0
Time=00:00
Cycle1 01 of 10
Total Time: 00h 56m
Remainig Time:00h 56m
select “Start” to continue. If the user
doesn’t make a decision within 100
Stop
Pause
Start
Skip
View File .
seconds, the system will continue running automatically.

Press “View File” to view edited PCR programs.

Press “Skip” to stop current temperature segment and run the next.
2.2 How to set system parameters
System Parameter Setting Interface
In the main interface press “System” to
enter
into
system
parameter
setting
interface.
Press “Enter” to move the cursor to the
next item. The option is followed by the
symbol “ ”. Press “Up/Down” to roll the
option for selecting, and other information
can be input by pressing “Digit&Letter”.
Press
“Right/Left”
to
move
cursor’s
position.

Hotlid=off
Date:
Time:
Default User:
Default File:
Control Mode:
Hotlid:
Key Sound:
Run End Sound:
Press “Back” to go back to the main
2007-11-1 10:05:00
03-10-22
14:53:19
PCR1
BOECO
Block
off
Yes
Yes
Back
Save
menu.

Press “Save” to save parameters.
Special Reminding: When set the parameter, please press the “Save”, otherwise the
parameter remains the original one.
2.2.1 “Date”,“Time” state the default system time after power-on.
2.2.2 “Default File”, “Default User” state the default running program and the default user name.
— 20 —
BOECO TC-PRO Thermal Cycler
Chapter Four Operation Guide
2.2.3 “Control Mode” states the default control mode, including two optional modes as “Block” and
“Tube”.
“Block” mode subjects to simple temperature control during the course of cooling & heating
only. Compared to the block, cooling & heating rate of reagent is a little slower, therefore,
the actual change of reagent’s temperature is different from the setting procedure. The
actual time for reagent to reach & keep the setting temperature is far shorter than the
setting time. (Please refer to the following figure: relation between block temperature and
reagent temperature)
Block
Reagent
“Tube mode” has considered the phenomenon that the cooling & heating rate is a little
slower than that of block. There is an overshoot after reaching the setting temperature,
which can allow reagent to reach the setting temperature faster. During the same setting
time as “Block”, the actual time for reagent to keep the setting temperature is prolonged
obviously.
(Please refer to the following figure: relation between block temperature and
reagent temperature)
Block
Reagent
2.2.4
“Hotlid” stand for the state of hotlid “Open” or “Close”.
2.2.5
“Key Sound”, “Run End Sound” stand for the sound of pressing key and reminding sound
after the procedure runs over respectively.
See details in the APPENDIX 2.
— 21 —
BOECO TC-PRO Thermal Cycler
NOTE:
1.
2.
3.
Chapter Four Operation Guide
After set the parameters, please press “Save” to confirm the setting,
otherwise the parameter remains the original one.
Before the instrument leaves the factory, its inside clock has been
adjusted in accordance with Beijing Time.
For the first use of the instrument, you are advised to confirm whether the
date and clock time are set right as local time.
3. How to run a double- block PCR program
Switch the power on, BOECO TC-PRO Thermal Cycler will beep twice, and it shows the power
supply is connected well. The screen will display “Self testing ••••••”,and the instrument will
carry on self testing. The self-testing needs about 1~2 minutes, and please wait patiently. If the
self-testing is successful, the screen will display the main interface.
Dual block means two blocks: Block A and B respectively represent actual block on the left and
right. Press “Shift” to operate A or B blocks in the same interface. The following introduction takes
the example of operating A block
The Main Interface
In the top center of the main interface,
it displays the current operating block.
As in the illustration it shows the
control symbol is “A”. Press “Shift” to
BOECO Version 2007-1.0
A
2007-11-1
10:05:00
A
Block:**×**well
Default User:BOECO
Default File: PCR1
Control Mode:Block
change control symbol. In the central
area of the main interface it displays
the types of A & B blocks, default file,
Self testing
default user, control mode and sample
volume.
If the current Control Mode is Block
B
Block:**×**well
Default User:BOECO
Default File: PCR2
Control Mode:Block
mode, press “Run” to enter into the
File Running Interface; While if the
current Control Mode is Tube mode,
Self testing
press “Run” and thus the following
dialog box will appear:

File
Press “File” to enter into the File List Interface.
Run
Press “System” to enter into System Parameter Setting Interface.

If the current file doesn't have code, press “Run” to enter into File Running Interface and tube

System
type confirmation; If the current file has code, press “Run”, thus there appears a dialog box.
Select Sample Volume:
050μl
— 22 —
BOECO TC-PRO Thermal Cycler
Chapter Four Operation Guide
The same as single module. Press “Back” to go back to the main interface. Press ”Accept” key to
confirm the code.

If the current Control Mode is block mode, press "Run" running into the file operation
interface; If the current control mode is Tube mode, press "Run" key, then pop-up the
following dialog box.
Select Sample Volume:
050μl
The same as single module, user could input the sample volume (Unit: Micro liter) according to
the practicality. Press “Back” to go back to the main interface. Press ”Run” key to to enter into file
running interface.
3.1 How to edit a PCR program
File List Interface
In the main interface press “File” to enter
into File List Interface. There are 3 files
listed in the form as showed in the picture.
When the flashing bar is flashing in the
User
Column,
select
users
through
BOECO Version 2007-1.0 A
2007-11-1 10:05:00
No.
User
File Name Save Time
1
BOECO1*
PCR1
2003-11-1
2
BOECO2
PCR2*
2003-11-1
3
BOECO3
PCR3
2003-11-1
pop-up key; at this time, the File Name
Column will display the relative files of the
selected user dynamically. Shift to the
B
Block: **×**well
File Name Column, where the flashing
Default User: BOECO
bar will flash, then select relative user’s
Default File: PCR2
file through pop-up key.
Control Mode: Block

Press “Edit” to edit the file indicated
Edit
by the cursor.
New File
Delete
Back
Run

Press “New File” to edit one new file.

Press “Delete” and the system will alarm “Confirm delete file?”, then according to the alert
information to select(Delete) or (BACK).

Press “Back” to go back to the main interface.

If the current file doesn't have code, press “Run” to enter into File Running Interface and tube
type confirmation; If the current file has code, press “Run”, thus there appears a dialog box.
Input Password
The same as single module. Press “Back” to go back to the main interface. Press ”Accept” key to
confirm the code. If the current Control Mode is block mode, press "Run" running into the file
operation interface; If the current control mode is Tube mode, press "Accept" key, then pop-up
tube vol. Settings dialog box.
— 23 —
BOECO TC-PRO Thermal Cycler

Chapter Four Operation Guide
If the current Control Mode is Block mode, press “Run” to enter into the File Running
Interface; while if the current Control Mode is Tube mode, press “Run” and thus the following
dialog box will appear:
Select Sample Volume:
050μl
The user can input Sample Volume (Unit: Micro liter) by the same way as that of the main
interface. Press “Back” to go back to File List Interface; Press “Run” to enter into file running
interface.
NOTE:
The sign“*”at the top of User and File indicates the current running file is
default one, which can be modified in the parameter setting interface.
File Edit Interface
In this interface move the cursor by
BOECO Version 2007-1.0
Seg. Temp(C)
Time
1c 95.0
01:00
2c 55.0
02:00
3c 72.0
01:45
4
04.0
00:00
pressing “Up/Down” and“Right/Left”, and
the position that the cursor reaches will be
displayed in white on a black background.
Press “Digit&Letter” to modify parameter
settings. Press “Function” to change +/-.

Press
“+Seg.”
Segment
segment,
Edit
you
to
enter
Interface.
can
into
In
set
the
A
2007-11-1 10:05:00
Ramp(C/s) +Temp +Time
#.#
+0.0
+0:00
#.#
+0.0
+0:00
#.#
+0.0
+0:00
#.#
+0.0
+0:00
Cycle1:×10 From 01 to 03
every
B
setting
Block: **×**well
Default User: BOECO
Default File: PCR2
Control Mode: Block
temperature (Temp.), duration (Time),
Ramping rate (Ramp), temperature
increment of every cycler(+Temp) and
time increment of every cycler (+Time).
+Seg.
Press “Delete” to delete the present
+Cycle
Delete
Back
Save
segment.

Press “+Cycle” to enter into Section Edit Interface, where you can set cycle numbers and
initial cycle segment. The figure shows a cycle from the first segment to the third one, and
there are totally 10 times cycles.

Press “+Cycle” repeatedly to add sections (max.: 5 sections ). Press “Delete” to delete the
present segment. Move the cursor by pressing “Up/Down” to enter the former segment or
the latter.

Press “Save” to enter file save interface.

Press “Back” to go back to the File List Interface.
Special Warning:
— 24 —
BOECO TC-PRO Thermal Cycler
Chapter Four Operation Guide
1. For the new edited PCR program, please save first then run it, otherwise the file cannot be run.
2. When setting “Time” as “--:--”, the hot lid will be closed automatically when the block reaches
the target temperature
File Save Interface
In this interface press “Digit&Letter” to
enter the file name. Press “Enter” to move
BOECO Version 2007-1.0
Abc
the cursor forwards one position. Select
pop-up key. Click left button to delete
characters while clicking right button to
capital or small letter, and there will be a
B
Block: **×**well
Default User: BOECO
Default File: PCR2
Control Mode: Block
capital or small letter indication on the left
top of the screen. (As the example: abc).

2007-11-1 10:05:00
User:
BOBOECO
File Name:
PCR1
Password:
PC****
Password Check:PC****
User , File Name and Password through
move the cursor. Press “Caps” to shift the
A
Press “Main” to go back to the main
interface.

Press “Save” to save the file. When the
Main
Caps
Save
Run
file is normally saved, the system will
remind “File have been saved. Please continue another operation”, Press enter key to
cancel the reminding bar. If the file was used, the system will remind you to rename
(Rename)or continue to save (Save).

If the current Control Mode is Block mode, press “Run” to enter into the file running interface;
while if the current Control Mode is Tube mode, press “Run” and thus the following dialog
box will appear:
Select Sample Volume:
050μl
The user can input Sample Volume (Unit: Micro liter) by the same way as that of the main
interface. Press “Back” to go back to File List Interface; Press “Run” to enter into the file running
interface.
Note:
Setting the password as 0~9 number character.
— 25 —
BOECO TC-PRO Thermal Cycler
Chapter Four Operation Guide
File Running Interface
After the file is running over, the system will
BOECO Version 2007-1.0
remind “File run over”.

Press “Stop” and it will remind “Confirm
stop
running?”.
According
to
File Name: PCR1
the
Seg. 1
PV
SV
reminding information to select “STOP”
or “START”.

Temp(C)
32.6
95.0
Total Time:00h 00m
pause
B
According
to
the
“START” to continue. If you don’t make
a decision within 100 seconds, the
will
continue
running
Stop
automatically.
Time(m:s)
00:00
01:00
Cycle1
01
01
Remain Time: 00h 00m
Hotlid=042C
Block: **×**well
Default User: BOECO
Default File: PCR2
Control Mode: Block
reminding information you can select
system
2007-11-1 10:05:00
User: BOECO
Press “Pause” and it will remind “Now
running?”
A
Pause

Press “View File” to view edited PCR programs.

Press “Skip” to stop current temperature and run the next.
Start
Skip
View File
3.2 How to set system parameters
System Parameter Setting Interface
In the main interface press “System” to enter
system parameter setting interface.
Press “Enter” to move the cursor to next
item. The option is followed by the symbol
“ ”. Press “Up/Down” to roll the option for
selecting, and other information can be
entered
information
by
pressing
“Digit&Letter”. Press “Right/Left” to move
BOECO Version 2007-1.0
Default User: BOECO
Default File: PCR1
Control Mode: Block
Sample Volume: 050μl
Key Sound:
Yes
Run End Sound: Yes
B
the cursor’s position.

Press “Back” to go back to the main
A
2007-11-1 10:05:00
Date: 03-10-22
Time: 14:53:19
Hotlid: 105C
Block: **×**well
Default User: BOECO
Default File: PCR2
Control Mode: Block
menu.

Back
Press “Save” to save the parameters.
Save
Special Reminding:
When set the parameter, please press the “Save”, otherwise the parameter remains the original
one.
— 26 —
BOECO TC-PRO Thermal Cycler
NOTE:
Chapter Four Operation Guide
After set the parameters, please press “Save” to confirm setting, otherwise the
parameter remains the original one
Before the instrument leaves the factory, its inside clock has been adjusted in
accordance with Beijing Time
For the first use of the instrument, you are advised to confirm whether the date
and clock time are set right as local time.
4. How to run a gradient block PCR program
Switch the power on, BOECO TC-PRO Thermal Cycler will beep twice, and it shows the power
supply is connected well. The screen will display “Self testing ••••••”,and the instrument will
carry on self testing. The self-testing needs about 1~2 minutes, and please wait patiently. If the
self-testing is successful, the screen will display the main interface.
Main Interface
On the left top of the main interface, the state
of Hot lid is displayed. On the central area,
Hotlid=off
2007-11-1 10:05:00
basic information such as Boeckel + Co
BOECO
(GmbH + Co) KG information, software
version number, block type, default file,
default user, control mode and sample
Boeckel + Co (GmbH + Co) KG
Version: 2007-1.0
Block:
Grad
Default User: BOECO
Default File: PCR1
Control Mode: Block
volume, are displayed.

Press “File” to enter into the File List
Interface.

Press “System” to enter into the System
Parameter Setting Interface.

If the current file doesn't have code,
File
System
press “Run” to enter into File Running
Run
Interface and tube type confirmation; If the current file has code, press “Run”, thus there
appears a dialog box.
Input Password
The same as single module. Press “Back” to go back to the main interface. Press ”Accept” key to
confirm the code.

If the current Control Mode is block mode, press "Run" running into the file operation
interface; If the current control mode is Tube mode, press " Run" key, then pop-up tube vol.
Settings dialog box.
Select Sample Volume:
050μl
— 27 —
BOECO TC-PRO Thermal Cycler
Chapter Four Operation Guide
4.1 How to edit a PCR program
File List Interface
Press “File” on the Main Interface to enter
into the File List Interface, there are 3 files
listed in the form as showed in the picture.
When the flashing bar is flashing in the User
Column, select users through pop-up key; at
Hotlid=off
No.
User
1
BOECO1
2
BOECO2*
3
BOECO3
2007-11-1 10:05:00
File Name
Save Time
PCR1
2007-11-1
PCR2*
2007-11-1
PCR3
2007-11-1
this time, the File Name Column will display
the
relative files of the selected user
dynamically. Shift to the File Name Column,
where the flashing bar will flash, then select
relative user’s file through pop-up key

Press “Edit” to edit the file indicated by
the cursor.

Edit
New File
Delete
Back
Press “New File” to edit a new file.

Run
Press Delete” and the system will alarm “Confirm delete file?”, then according to alert to
select(Delete) or (BACK).

Press “Back” to go back to the main interface.

If the current Control Mode is Block mode, press “Run” to enter into the File Running
Interface; While if the current Control Mode is Tube mode, press “Run”, thus the following
dialog box will be displayed:
Select Sample Volume:
050μl
The user can input Sample Volume (Unit: Micro liter) by the same way as that of the main
interface. Press “Back” to go back to File List Interface; Press “Run” to enter into the file running
interface.
Note:
The sign“*”at the top of User and File indicates the current running file is default
one, which can be modified in the parameter setting interface.
— 28 —
BOECO TC-PRO Thermal Cycler
Chapter Four Operation Guide
File Edit Interface
In this interface move cursor by pressing
Hotlid=off
“Up/Down” and “Right/Left”, and the
temperature increment of every cycler
100
75
50
25
0
Temp 1c 95.0 2c 55.0
Time
01:00
02:00
Seg. Temp(C)
Time
1c
95.0
01:00
2c
55.0
02:00
3c
72.0
01:45
4
04.0
00:00
(+Temp) and time increment of every
Cycle1:×10
position that the cursor reaches will be
displayed in white on a black background.
Press “Digit&Letter” to modify parameter
settings. Press “Function” to change +/-.

2007-11-1 10:05:00
Press “+Seg.” to enter segment edit
interface. In every segment, you can
set
setting
temperature
(Temp.),
duration (Time), Ramping rate (Ramp),
cycler (+Time). Press “Delete” to
+Seg.
3c 72.0
04.0
00:00
Ramp(C/s) +Temp +Time Grad.
#.#
+0.0
+0:00
02
#.#
+0.0
+0:00
02
#.#
+0.0
+0:00
02
#.#
+0.0
+0:00
00
From 01 to 03
+Cycle
4
01:45
Press ‘Enter’ To View Delails:
Delete
Back
Save
delete the present segment.

Press “+Cycle” to enter section edit
Hotlid=off
interface, where you can set cycle
100
75
50
25
0
Temp 1c 95.0 2c 55.0
Time
01:00
02:00
Seg. Temp(C)
Time
1c
95.0
01:00
numbers and initial cycle segment.
The figure shows a cycle from the first
segment to the third one, and there
are totally 10 times cycles.

Press “+Cycle” repeatedly to add
sections (max.: 5 sections ). Press
“Delete”
to
delete
the
1
present
to
enter
the
3
4
5
3c 72.0
4
01:45
04.0
00:00
Ramp(C/s) +Temp +Time Grad.
#.#
+0.0
+0:00
04
6
7
8
9
10
11
12
95.0 95.1 95.2 95.4 95.7 95.9 96.1 96.4 96.6 96.8 96.9 97.0
segment. Move cursor by pressing
“Up/Down”
2
2007-11-1 10:05:00
Back
former
k
segment or the latter.

Press “Save” to enter file save interface.

Press “Enter ” to check detailed gradient distributing situation. Please refer to the following
picture:

Press “Back” to enter file run interface.
Special Warning:
1. For the new edited PCR program, please save first then run it, otherwise the file cannot
be run.
2. When setting “Time” as “--:--”, the hot lid will be closed automatically when the block
reaches the target
temperature
— 29 —
BOECO TC-PRO Thermal Cycler
Chapter Four Operation Guide
File Save Interface
In this interface press “Right/Left” to enter
the file name. Press “Enter” to move the
cursor forwards one position. Select User ,
Hotlid=off
2007-11-1 10:05:00
abc
File Name and Password through pop-up
key. Click left button to delete characters
BIOBOCEO
while clicking right button to move the
User:
cursor. Press “Caps” to shift the capital or
File Name:
small letter, and there will be a capital or
small letter indication on the left top of the
screen. (as the example: abc).

PC****
Password Check:PC****
Password:
Press “Main” to go back to the main
interface.

PCR1
Caps
Main
Save
Run
Press “Save “to save the file. When the
file is normally saved, the system will remind “File have been saved. Please continue another
operation”, Press “Enter” to cancel the reminding bar. If the file was used, the system will
remind you to rename (Rename) or continue to save (Save).

If the current Control Mode is Block mode, press “Run” to enter into the File Running
Interface; While if the current Control Mode is Tube mode, press “Run”, thus the following
dialog box will be displayed:
Select Sample Volume:
050μl
The user can input Sample Volume (Unit: Micro liter) by the same way as that of the main
interface. Press “Back” to go back to File List Interface; Press “Run” to enter into the file running
interface.
Note:
Setting the password as 0~9 number character.
When the file is normally running, “•••” at
“Now Running” will continuously flash.
When running is over, the system will
remind “File run over”

Press
“Stop” and
it
will
remind
“Confirm stop running?”. According to
the reminding information to select
“STOP” or “START”. If the user make
no choice, the system will continue to
run within 100 seconds automatically.

Press “Pause” and it will remind “Now
pause running”. According to the
File Running Interface
Hotlid=off
100
75
50
25
0
Temp
Time
1c
2007-11-1 10:05:00
95.0
01:00
2c 55.0
3c 72.0
4 04.0
02:00
01:45
00:00
File Name:PCR1
User: BOECO
Now Running•••
1c
Time=00:00
Cycle1 01 of 10
1 2 3
4
5 6
7 8
9 10 11 12
95.0 95.1 95.2 95.4 95.7 95.9 96.1 96.4 96.6 96.8 96.9 97.0
Total Time: 00h 56m
Stop
Pause
— 30 —
Remaining Time:00h 56m
Start
Skip
View File
BOECO TC-PRO Thermal Cycler
Chapter Four Operation Guide
reminding information you can select “START” to continue. If you don’t make a decision
within 100 seconds, the system will continue running automatically.

Press “View File” to view edited PCR programs.

Press “Skip” to stop current temperature and run the next.

Press “Enter” key checking the grads temperature value.
4.2 How to set system parameters
System Parameter Setting Interface
In the main interface, press “System” to enter
system parameter setting interface
Press “Enter” to move the cursor to next item.
The option is followed by the symbol “ ”. Press
“Up/Down” to roll the option for selecting, and
other information can be entered information by
pressing “Digit& Letter”. Press “Right/Left”
to move the cursor’s position.

Press “Back” to go back to the main
interface.

Hotlid=off
10:05:00
2007-11-1
Date:
03-10-22
Time:
14:53:19
Default User: BOECO
Default File:
PCR1
Control Mode: Block
Hotlid:
off
Key Sound:
Yes
Run End Sound: Yes
Press “Save” to save the parameters.
Back
Save
Special Reminding: When set the parameter, please press the “Save”, otherwise the
parameter remains the original one.
NOTE:
After set the parameter, please press “Save” to confirm setting, otherwise the
parameter remains the original one.
Before the instrument leaves the factory, its inside clock has been adjusted in
accordance with Beijing Time.
For the first use of the instrument, you are advised to confirm whether the date
and clock time are set right as local time.
4.3 Characteristics of Temperature Distributing of Gradient Block
The gradient block adopts 3-group temperature control units, so that the gradient temperature
appears curve distribution as showed in the following figure (Setting temp is 40℃, and gradient
setting temp is 20 ). The gradient distribution among every block’s hole temperature is not even;
the temperature difference in mid of block is larger than that of its outer. Therefore, please refer to
the temperature displayed on the machine for every hole’s temperature when operating gradient
block.
— 31 —
BOECO TC-PRO Thermal Cycler
Chapter Four Operation Guide
Temperature[ ! ]
Gradient graphs
60
58
56
54
52
50
48
46
44
42
40
1
2
3
4
5
6
7
8
9
10
11
12
Colum n(left to right)
5 How to Input and Output the PCR Program
5.1 Connect Usb Flash Memory
Usb Link Interface
Press “usblink” key in the main interface to
enter the usb usblink interface. If insert usb
Hotlid=off
2007-11-1 10:05:00
flash memory, display as the left fig.

Press “Back” to go back to the main
interface.

Press “Input” key to choice input file from
The Insturment has Connected UsbDisk.
Please Choose Input or Output File.
usb flash memory, the equipment display
usb user list.

usb flash memory, the equipment display
Press F1 to Back
Press F4 to Input File
the local file user list.
Press F5 to Output File..
Press “output” key to choice export file to
Back
— 32 —
Input
Output
BOECO TC-PRO Thermal Cycler
Chapter Four Operation Guide
Usb Link Error Interface
If no inserted usb flash memory, display as the
Hotlid=off
2007-11-1 10:05:00
left fig. Press “Back” key can return back to the
main interface.
No disk connection。
Press F1 to Back
Back
5.2 Input file from usb flash memory

Usb User List
Press “Back” key can return back to the usb
link flash memory interface.

Press cursor up/down moving key to choice
the copay file user.

k
Hotlid=off
2007-11-1 10:05:00
User1
User2
If the user number bigger than 30, press
cursor left/right key to previous/next page.

Press “view” key to display usb file list.
Press Left / Right Key to Previous / Next Page.
Back
View
Usb File Fist
Press “Back” key can return back to usb flash
Hotlid=off
2007-11-1 10:05:00
memory user list interface.

Press cursor up/down moving key to choice
the copay file

File1
File2
If the file number bigger than 30, press
cursor left/right key to previous/next page.

Press “copy” key to input file.

If copy correct, display the following dialog
box.
Press Left / Right Key to Previous / Next Page.
File Saved
Back
otherwise display:
File input error.
— 33 —
Copy
k
BOECO TC-PRO Thermal Cycler
Chapter Four Operation Guide
5.3 Export file to usb flash memory
Equipment User List

Press “Back” key to return back the usb
link flash memory interface.

Press cursor up/down moving key to
choice the copay file user.

Hotlid=off
2007-11-1 10:05:00
Local
User1
User2
If the user number bigger than 30, press
cursor left/right key to previous/next page.

Press “view” key to display usb file list.
Press Left / Right Key to Previous / Next Page.
Back
View
Equipment File List

Press “Back” key can return back to
equipment user list interface.

Press cursor up/down moving key to
choice the copay file.

Hotlid=off
2007-11-1 10:05:00
Local
File1
File2
If the file number bigger than 30, press
cursor left/right key to previous/next page.

If copy correct, display the following
dialog box.

Press “view” key to display usb file list.
File Saved
Press Left / Right Key to Previous / Next Page.
Back
Otherwise display
File output error.
— 34 —
Copy
k
BOECO TC-PRO Thermal Cycler
Note:
Chapter Four Operation Guide
Usb read-write user number is 500, every user’s file number is 2000.
Usb read-write fixup list ,usb root list descended BOECO folder.
Every user is one son folder of BOECO folder.
Forbid to insert and pull out usb flash memory very fast. To protect apparatus
and usb flash memory, if insert and pull out usb flash memory, the apparatus
will close usb function until the next boot-strap.
The operation method for double module is the same, the link of usb flash
memory is only for two module under the original interface.
— 35 —
BOECO TC-PRO Thermal Cycler
Chapter Five Connection Computer
Chapter Five Connection of Computer
1. Software installation
Click “Setup.exe” to lnstallation TC-PRO Program.
2. Online setup
TC-PRO has configurable embedded internal networking modules, provide the corresponding
tools set.
3. Software installation
The first step:click to run need_setup.exe.
The second step: click next button.
— 36 —
BOECO TC-PRO Thermal Cycler
Chapter Five Connection Computer
The third step: choice installtion list, click next button.
The fourth step: click next button.
— 37 —
BOECO TC-PRO Thermal Cycler
Chapter Five Connection Computer
The fifths click install button.
The sixth step: installation finished, clich finish button.
4. Configure your equipment
4.1 Network Connection
Link the equipment and computer lines through the network connection (Cross, Class B
— 38 —
BOECO TC-PRO Thermal Cycler
Chapter Five Connection Computer
Connection)
RJ45 connection
Computer
Instrument
4.2 Network Enable Administrator
Search button
Configure
Mapping
Function list
— 39 —
BOECO TC-PRO Thermal Cycler
Chapter Five Connection Computer
4.3 Configure
Click the Search button, the device will search tool automatically.
Searched equipment IP
Double-click the searched IP on the column, open the attribute page and choice the network
option.
Input IP address
Input netmash
— 40 —
BOECO TC-PRO Thermal Cycler
Chapter Five Connection Computer
Open the attribute page and choice operrating mode option then click setting button.
The operating mode choice TCP server mode.
Choose TCP
Server Mode
Single click ok button, finish IP address setting.
— 41 —
BOECO TC-PRO Thermal Cycler
Chapter Five Connection Computer
Single click function list” COM MAPPING”.
Add object
equipment
OK
Delete object
equipment
Click “add object equipment” button.
— 42 —
BOECO TC-PRO Thermal Cycler
Chapter Five Connection Computer
Double-click on the column searched IP, setting the mapping attributes page, select Advanced
Settings, setup as the following fig.
Choice Serial Parameters, setup as the following fig.
— 43 —
BOECO TC-PRO Thermal Cycler
Chapter Five Connection Computer
Click ok button, and click OK button of the application settings, and click the Remove equipment,
completed mapping settings.
Note:
1
2
3
4
5
The IP address is not unanimous when multiple devices interconnection.
In the first use, re-equipped the equipment.
Need to set up the same subnet when multiple equipment networking.
Device does not support automatic ip address functions.
Multiple equipment networking can use switches, such as the use of
cross-HUB requires the use of network connectivity.
5.Main interface
— 44 —
BOECO TC-PRO Thermal Cycler
Chapter Five Connection Computer
6. Toolbar
Amend user name
Output user
Input user
Input file
Paste user
Copy user
Setup user
Copy file
New file
Change file code
Delet file
Grads calculate
Link equipment
Save file
Equipment setup
Paste file
Edit file
Disconnect link
Change file name
Copy user
Delet user
7. Basic manipulation
Run a file
1. Open the file of the equipment or the machine.
2. Click Run button.
3. Select test-tube type and size of the test tube.
4. Click ok button operation.
Clew:
If the file is saved in the computer, pre-operational options related equipment operation.
Checking the operating file
1. Document if the equipment running, the device will be displayed from the bottom Run File
(A / B) Options
2. Double-click Run File (A/B),display the operation file content and equipment running states.
Clew:
By clicking Pause / Stop button to pause / equipment running.
— 45 —
BOECO TC-PRO Thermal Cycler
Chapter Five Connection Computer
Check history information
Double-click the historical option in the System option to show the historical information.
— 46 —
BOECO TC-PRO Thermal Cycler
Chapter Five Connection Computer
Checking the equipment storage state
Double-click the System to view equipment storage condition.
Setup the system parameter
Double-click the setting option of the
System options to set-up system parameters.
— 47 —
BOECO TC-PRO Thermal Cycler
Chapter Five Connection Computer
8. User manual
How..
Create new users
Change the user name
Copy user
Paste user
Delete user
Imput user
Export user
Create a new user
1. Choose the name of the equipment list or any user name.
2. Choice the create user menu in “User” menu, pop-up user dialog box.
3. Enter your user name, click ok button.
— 48 —
BOECO TC-PRO Thermal Cycler
Chapter Five Connection Computer
Change the user name
1. Choice change the user name option.
2. Choice the “Change User Name”menu under the User menu, pop-up the “Change User
Name” dialog box.
3. Import user name, click the ok button.
Clew:
User can’t change the user name when the file of this user is opening.
Copy user
1. Choice the user name needed to copy.
2. Choice the “Copy User”menu under User menu.
Paste user
1. Choose the name of the equipment list or any user name.
2. Choice the “Paste User” menu under “User”menu.
Clew:
1. If the copy user already occurs, and the file is opening, can’t been plaster user.
2. Only can plaster between the local computer and equipment.
— 49 —
BOECO TC-PRO Thermal Cycler
Chapter Five Connection Computer
Delete user
1. Choice the user need to be deleted.
2. Choice the “delete User” menu under “User” menu.
Clew:
Can’t delete user when the file open.
Imput user
1. Choice the local computer.
2. Choice the Import User menu under the User menu.
3. Choice the imput list, click the open button.
Clew:
Can’t imput user if the existent user is opening.
Output user
1. Choice the user which want to export in the local computer.
2. Choice “Export User”menu under “User”menu.
3. Choice the export list, click ok button.
Clew:
Can’t export user when the file open
9. File menu
How…
Create new file
Open and edit file
Copy file
Paste file
— 50 —
BOECO TC-PRO Thermal Cycler
Chapter Five Connection Computer
Delete file
Change file name
Change the file password
Save file
Imput file
Export file
Create new file
1. Choice the user which need to found new file.
2. Choice the “Create New File” menu under File menu, pop-up the dialog box.
3. Imput the file name and password, click ok button.
Edit file
1. Double click the equipment list user option, open the file list and double click edit file or create
new file.
2. Edit the file content, click “Save” button to save the file.
Clew:
May examine the whole steps under the figure.
When user edit the file, if the file has password, user must input the password then edit the file.
— 51 —
BOECO TC-PRO Thermal Cycler
Chapter Five Connection Computer
Segment parameter setup
Circle parameter setup
Setting detail
Copy file
1. Choice the file needed to copy.
2. Choice the “Copy File”menu under File menu.
Paste File
1. Choose the user option under the equipment list.
2. Choice the “Paste File” menu under “File ”menu.
Clew:
Only can paste file between computer and equipment.
Can’t cover the opened existent file.
— 52 —
BOECO TC-PRO Thermal Cycler
Chapter Five Connection Computer
Delete File
1. Choice the file need to delete.
2. Choice the “Delete File”menu under the File menu
Clew:
Can’t delete the opened file.
Change the file name
1. Choice the file which user want to change the name.
2. Choice the “Change File Name” menu under File menu.
3. Input file name, click OK button.
Clew:
Can’t change the opened file name.
Change the file password
1. Choice the file which want to change the password.
2. Choice the “Change File Password”menu under “File”menu, pop-up dialog box.
3. Input the old password, the new password and click OK button.
Clew:
Can’t change the opened file name.
— 53 —
BOECO TC-PRO Thermal Cycler
Chapter Five Connection Computer
Save file
Clich “Save File”option to save the file after modification.
Imput file
1. Choice the local computer user option.
2. Choice “Export File” menu under the File menu.
3. Choice the file, click open button.
Clew:
Can’t imput the opened file.
Export File
1. Choice the file need to export.
2. Choice the “Export File” men under “File” menu.
3. Choice the export outlet, click OK button.
— 54 —
BOECO TC-PRO Thermal Cycler
Chapter Five Connection Computer
Clew:
Can’t export the opened file.
10. Tool menu
How…
Connect equipment
Disconnection
Grads calculate
Change equipment ip and name
Connect equipment
Choice the equipment, click “Connect”menu.
Clew:
One equipment can only connect one computer at the same time.
Disconnection
Choice the equipment, click the “Disconnect”menu.
Grads Menu
1. Choice the grads menu.
2. Import the grads origination point, and the grads value.
3. Choice the computer module type 8x12(96well)16x24(384well).
4. Click Calc. Button.
— 55 —
BOECO TC-PRO Thermal Cycler
Chapter Five Connection Computer
— 56 —
BOECO TC-PRO Thermal Cycler
Chapter Five Connection Computer
Change the equipment IP and the name
1. Choice the “Server Setting” option under “tool” menu, pop-up the setting dialog box.
2. Click the “Add” button to add a row.
3. Click the “Delete” button to delete the current row.
4. Put the ip address and alias, click “Save” button to save the setup.
Clew
1. The setup will go into effect until the software restart.
2. Ip setup should be corresponding to the equipment.
— 57 —
BOECO TC-PRO Thermal Cycler
Chapter Six Firmware Upgrade
Chapter Six Firmware Upgrade
1.Configure your equipment
Connect the equipment and computer lines through the network (cross, then Class B).
RJ45 connection
Computer
Instrument
2. How to Upgrade the Software
1)
Open apparatus power to start instrument, hold down the F1 key at the same time, then
equipment enter the software upgrade readiness estate,the apparatus display “Prepare to
upgrade • • •”.
2)
Operate the procedure Network Enable Adminastrator. Equipment will use virtual equipment
mapping for communications during upgrades.
Lookup
Mapping
configuration
mamapin
g
Function list
— 58 —
BOECO TC-PRO Thermal Cycler
3)
Chapter Six Firmware Upgrade
Configuration
Click the Search button, the device will search tool automatically.
searched IP address
Double click the searched IP address, open the property page to choice the network option.
— 59 —
BOECO TC-PRO Thermal Cycler
Chapter Six Firmware Upgrade
Open Operrating Modemenu, click settings, open the property page to choice operrating mode and
click setting button.
Choice “Real Com Mode” in operation Mode.
Choice Real Com
Mode
— 60 —
BOECO TC-PRO Thermal Cycler
Chapter Six Firmware Upgrade
Click the function list ”COM MAPPING” option.
Input target
equipment
OK
Output target
equipment
Click to add target equipment button.
— 61 —
BOECO TC-PRO Thermal Cycler
Chapter Six Firmware Upgrade
Double click the searched IP address to set the mapping property page, choice “Advanced Setting”, as
below:
Choice “Serial Parameters”, as below:
— 62 —
BOECO TC-PRO Thermal Cycler
Chapter Six Firmware Upgrade
Click ok button, application configuration
4) Run upgrade exe.
5) Press “open” key, choice the upgrade procedure in the computer.
6) Press “open” key, the computer start to upgrade the software of instrument.
7) Software under upgrade, the apparatus will clue on “Configuring Mode • • •”.
8) After the software upgrade finish, the apparatus will clue on “Upgrade finished • • •”.
9) Firmware upgrade finished.
10) Click remove equipment, to remove the equipment mapping.
11) Resume configuration.
Network Enable Adminastrator Choice configuration function.
configuration
— 63 —
BOECO TC-PRO Thermal Cycler
Chapter Six Firmware Upgrade
Double click the searched IP address, open the property page to choice the network option.
Operating mode choice “TCP Server Mode”.
Choice TCP
Server Mode
Click Ok button, close Network Enable Adminastrator.
— 64 —
BOECO TC-PRO Thermal Cycler
Chapter Six Firmware Upgrade
12) Restart the apparatus after closing, enter the system parameter configuration interface to
reinstall system parameter.
Note:
Upgrade procedures will not be noticed in additional, users can land website
www.BOECO.com and download the latest version for the procedure.
— 65 —
BOECO TC-PRO Thermal Cycler
Chapter Seven Failure Analysis and Troubleshooting
Chapter Seven Failure Analysis and Troubleshooting
In this chapter, we briefly represent possible failures, reason analysis and troubleshooting
1. Failure Analysis and Troubleshooting
No.
Phenomenon
Failure Analysis
Disconnected
power supply
1
No display and the two beeps missed
when power on
Bad fuse
Switch failure
Troubleshooting
Check power supply for
correct connection
Replace it (250V 8A
Φ5×20mm)
Replace it
Others
After power on, the instrument beeps
twice, and then the LCD display:
“Please insert Block!”, with beep
warning sound.
Error Message when running: “Please
check error” with any information
below:
“Temperature sensor disconnect”
“Temperature sensor1 disconnect ”
“Temperature sensor2 disconnect ”
“Temperature sensor3 disconnect ”
Bad connection
between
the
block and the
main body.
4
Error Message when running: “Please
check error” with the information like
“Heat sink sensor disconnect”
Bad
sensor
connection
or
sensor broken
5
Error Message when running: “Please
check error” with the information like
“Hotlid sensor disconnect”
Bad
sensor
connection
or
sensor broken
2
3
Bad
sensor
connection
or
sensor broken
Ventilation jam
6
Abrupt change in heating speed or
bad temperature control accuracy
Bad connection
Cooling
failure
— 66 —
module
Power off, pull out the
block, and then re-insert
the block, and power on.
Power off, pull out the
block, and then re-insert the
block, and power on.
Contact
distributor
manufacturer
or
Power off, pull out the
block, and then re-insert the
block, and power on.
Contact
distributor
or
manufacturer
Power off, pull out the
block, and then re-insert the
block, and power on.
Contact
distributor
or
manufacturer
Clear the jam
Open the device, check the
connectors for reliable
connection
Contact distributor or
manufacturer
BOECO TC-PRO Thermal Cycler
7
8
9
Chapter Seven Failure Analysis and Troubleshooting
Significant change in cooling speed,
or unavailable temperature below
ambient one
The block cannot be heated or cooled.
Lid cannot be heated.
The
ambient
temperature or
humidity is too
high, which is
beyond
the
normal working
condition of the
instrument.
Cooling module
failure
Fan failed or
stopped
Bad temperature
sensor.
Malfunction of all
cooling modules
Set the hot-lid
as ”OFF” in the
system
parameter
setting interface
The control
temperature time
is set as “--:--” in
the file edit
interface, which
lead to the hotlid
closes
automatically.
Loose
connection
between
connectors
Lid heater failure
Please
regulate
the
ambient temperature and
the humidity.
Contact distributor or
manufacturer
Contact
distributor
manufacturer
or
Set the hotlid’s state as a
certain temperature value.
Set the temperature control
time in digital.
Contact
distributor
manufacturer
or
Contact
distributor
manufacturer
or
Contact
distributor
manufacturer
or
Lid sensor failure
10
Abnormal characters displayed in the
LCD
11
Inactive keys
12
The setup of parameters is invalid
Bad contact of
chip
with
its
socket
Chip
malfunctioning
Film panel
destroyed
Failure to press
“Save” button
after the setup of
parameters
— 67 —
Please refer to Section 1.2,
2.2 and 3.2 of Chapter 4.
BOECO TC-PRO Thermal Cycler
Reagent in
evaporated
13
Note:
the
reaction
Chapter Seven Failure Analysis and Troubleshooting
tubes
Failure to set
hot-lid
temperature, but
set the hot-lid
as ”OFF”
The
reaction
tubes are placed
unevenly
The
lid
of
reaction
tubes
are
closed
untightly
Please close the lid of
tubes tightly before put
them into the instrument.
During warranty terms, please contact distributor or manufacturer if there is a need
to open the device to check a failure listed in the above table.
Each instrument with the same or different brand has their own control temperature
features, including cooling & heating rate, stability and fluctuation; meanwhile, the
biological experiment has its own features such as indeterminacy, and easy to be
affected by the surroundings, therefore, any PCR program that is achieved on one
instrument successfully, maybe cannot be achieved the same successful result.
Once you change instruments to do the same experiment, you’d better adjust the
PCR running program, so as to get an ideal state.
2. Spare Parts list
NO.
Please refer to 《How to set
the parameters 》 in the
Section 1.2, 2.2, 3.2 of
Chapter 4, to set the
temperature of hot-lid.
Adjust the hole location of
reaction tubes, and place
the tubes symmetrically.
Symbol
Name
1
BYQ505801100000
Driving board
2
BYQ505801200000
CPU board
3
BYQ505801410000
Transform parts
4
BYQ605802000000
Block parts
5
BYQ154314200010
Fuse
6
BYQ165200000020
Resistance
7
BYQ902102100070
Peltier
8
BYQ152303000250
Power Plug
9
BYQ155420000070
LED display
10
BYQ905801300010
Touch panel
11
BYQ158206000040
Power supply
12
BYQ905801300020
Touch panel shell
— 68 —
BOECO TC-PRO Thermal Cycler
Appendix 1
Appendix 1 Wiring Diagram
(This diagram is regarded only as a reference, and it is a subject to change without prior
notice. We apologize for any inconvenience caused.)
PCB1
Driver Board
BYQ505801100000
J1-1_HV+
J1-2_HV-
PCB2
BYQ505801400000
1#FFC
J2-1_TE1+
J2-2_TE1J2-3_TE2+
J2-4_TE2-
2#FFC
J4-1_V+
J4-2_V-
N44110S
J_PLUS1-1
J_PLUS1-2
J_PLUS1-3
J_PLUS1-4
J_PLUS1-5
J_PLUS1-6
J7-CPU-34P
J2-13_+5V
J2-14_GND
D2MV-01L22-1C3
USB-1
USB-2
USB-3
USB-4
USB-5
1
NC 2
NO
COM
AT-320240Q3
1
J7_LCD_24P
J1_KEY_10P
J20_POWBOARD_40P
J22-1_V+
J22-2_V-
FAN1
J23_GND
DC+
DC-
J5-CPU-34P
3#FFC
SWITCH1
36V600W
C1
AC/DC
C2
V+
V+
VVPE
N
AC100-240V L
INLET1
FUSE1
NO1
FUSE FUSE
E
NO2
U1
FUSE FUSE
FUSE2
PE
POWER SYSTEM
— 69 —
L
N
J15_CPLD_BURN
J3_IDC20_BURN
1
J3-1_SW
J3-2_SW
J3-3_SW
AC/DC1
VB
CB
V+
V+
VV-
J18-1_GND
J18-2_GND
J18-3_ROOMSENSER
KEYBOARD
J2-1_SW
J2-2_SW
J2-3_SW
3
J11_BLK&ZJB_FCC34P
LCD1
!"#$%
CPU Board
BYQ505801200000
1
2
J5-1_TE1+
J5-2_TE1J5-3_TE2+
J5-4_TE2-
J8-1_MOT1
J8-2_MOT2 J6-1_24VFAN+
J8-3_MOT3 J6-2_24VFANJ8-4_MOT4
SW1
PCB3
SENSER1
J9-1_LAN
J9-2_LAN
J9-3_LAN
J9-4_LAN J4_BLOCK-48P
J9-5_LAN
J9-6_LAN
J1-2_RxD0
J1-3_TxD0
J3-1_HVJ3-2_NC
J3-3_ON/OFF
J6-ZJB-34P
J1-POW-34P
LAN1
J42-1_3.3V+
J42-2_GND
J42-3_CS
J42-4_DIR
J42-5_HV+
J42-6_HV-
Connect Board
L
E
N
AC INPUT
100-240V_
J6-1_USB-VCC
J6-2_USB-DM1
J6-3_USB-DP1
J6-4_USB-GND
J6-5_USB-GND
J6-6_USB-GND
BOECO TC-PRO Thermal Cycler
Appendix 2
Ite
m
1
Appendix 2
Parameter Value & Meaning
Parameter
Meaning
Range
Temp(C)
Temp. Control point
Time
Time
of
Temp.
Control(mm:ss)
Note
4.0~99.0
--:-2
3
Ramp(C/S)
4
+Temp
5
+Time
Cycle1
×00
6
From 00
to 00
Ramping Rate
(℃/Second)
Temp. Gain of Every
Cycler
Time Gain of Every
Cycler(mm:ss)
Section Numbers
Cycler Numbers from
beginning to the end
The start segment
of the section
The end segment
of the section.
The time of Temp. control is
( set the value before long limitlessly, meanwhile, the
“:”through “Function” key.) hotlid closed.
00:00~59:59
0.1 ~2.0
#.#
Maximum of Ramping Rate
0.0~9.9
0:00~9:59
1~5
1~99
X(and existed segment
no.)
Cycle1:×00 From 00 to 00
≤X+15
7
Grad.
Gradient
Temp.
Difference(℃)
0 ~30
8
Date
Date(YY-MM-DD)
00(~99)-01(~12)-01(~31) year-month-date
9
Time
10
Default File
11
Default User
Time(HH:MM:SS)
The default file
name
The default user
name
00:00:00~23:59:59
Max. length is
bytes.
Max. length is
characters.
Block
12
Control Mode
Control Mode
Tube
13
Sample Volume
14
Holid
15
Key Sound
16
Run End Sound
hour:minute:second
11
11
Under
this
mode
(once
reaching target temperature
namely entering into the
thermostatical state), the time
for
reagent’s
temperature
under target temperature is
less than setting time (see Fig.
1).
Under
this
mode
(once
entering into the thermostatical
state after reaching target
temperature and decreasing
about
3 ℃ ),
reagent’s
temperature can be close to
target temperature very fast
(see Fig. 2).
Sample Volume(ul) 10~200
State of Hot Lid: Off
Off
State of Hotlid:
Open( )
30~110
Key Sound (Yes or
No)
Run End Sound (Yes
or No)
Yes/No
Humming
Yes/No
Humming continuously
— 70 —
BOECO TC-PRO Thermal Cycler
Appendix 2
Block
Block
Reagent
Reagent
Fig.1
Fig.2
— 71 —
NOTE:
Boeckel + Co. (GmbH + Co.) KG
P.O.Box 11 22 26
Roedingsmarkt 33, 20459 Hamburg
20422 Hamburg, Germany
Tel. +49-40-32 56 27-27
Fax. +49-40-32 56 27-41
Website: www.boeco.com
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