Ettan Spot Handling Workstation
GE Healthcare
Ettan Spot Handling Workstation
System Manual
Ettan Spot Handling Workstation 2.1
Important user information
All users must read this entire manual to fully understand the safe use of Ettan Spot Handling Workstation.
WARNING!
The WARNING! sign highlights instructions
that must be followed to avoid personal injury.
It is important not to proceed until all stated
conditions are met and clearly understood.
WARNING! Avoid exposure to hazardous laser
radiation.
CAUTION!
The Caution! sign highlights instructions that
must be followed to avoid damage to the
product or other equipment. It is important not
to proceed until all stated conditions are met
and clearly understood.
Note
The Note sign is used to indicate information
important for trouble-free and optimal use of
the product.
CE Certifying
This product meets the requirements of applicable CEdirectives. A copy of the corresponding Declaration of
Conformity is available on request.
The CE symbol and corresponding declaration of
conformity, is valid for the instrument when it is:
– used as a stand-alone unit, or
– connected to other CE-marked GE Healthcare
instruments, or
– connected to other products recommended or
described in this manual, and
– used in the same state as it was delivered from
GE Healthcare except for alterations described in this
manual.
Recycling
This symbol indicates that the waste of
electrical and electronic equipment must not
be disposed as unsorted municipal waste
and must be collected separately. Please
contact an authorized representative of the
manufacturer for information concerning the
decommissioning of your equipment.
WARNING!
This is a Class A product. In a domestic environment
this product may cause radio interference in which
case the user may be required to take adequate
measures.
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Contents
Contents
List of figures ................................................................................................................ 7
1 Introduction
1.1
1.2
1.2.1
1.3
1.4
1.5
1.5.1
1.5.2
1.5.3
1.5.4
The System Manual ............................................................................... 9
System overview ................................................................................. 10
Rating label............................................................................................. 13
Package contents ................................................................................ 14
Accessories ............................................................................................. 15
Associated documentation ............................................................. 15
Ettan Spot Handling Workstation Safety Handbook .......... 15
Ettan Spot Handling Workstation Site Preparation Guide 15
Ettan Spot Handling Workstation User Manual .................... 15
Ettan Spot Handling Workstation Online Help ...................... 15
2 System description
2.1
2.2
2.2.1
2.2.2
2.2.3
2.3
2.3.1
2.3.2
2.3.3
2.4
2.4.1
2.4.2
2.4.3
2.4.4
2.5
2.6
2.6.1
2.6.2
2.6.3
2.7
2.7.1
2.7.2
2.7.3
2.7.4
General ..................................................................................................... 17
The Cabinet ............................................................................................ 19
Front panel controls and indicators ........................................... 21
External controls and connections ............................................. 23
Safety switches .................................................................................... 23
The Spot Picker & Spotter ................................................................ 24
The picker head .................................................................................... 26
Video camera ........................................................................................ 27
The spotter head.................................................................................. 28
The digestion system......................................................................... 29
The multiple probe head .................................................................. 30
The dryer ................................................................................................. 31
The ion generator ................................................................................ 32
The incubator ........................................................................................ 33
The transfer robot ............................................................................... 35
The hotel .................................................................................................. 36
Gel hotel ................................................................................................... 36
Microplate/MS sample tray hotel ................................................ 36
Barcode reader ..................................................................................... 37
Workflow in Ettan Spot Handling Workstation ..................... 38
Batch run start ...................................................................................... 38
Spot picking ............................................................................................ 38
Digestion .................................................................................................. 40
Spotting (standard method)............................................................ 44
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Contents
3 Start-up and shutdown
3.1
3.2
3.3
3.4
3.5
3.5.1
Starting the workstation .................................................................. 45
Before batch start-up ........................................................................ 46
Pause batch mode .............................................................................. 46
Shutting down the workstation .................................................... 47
Emergency shutdown ....................................................................... 47
Reset the Emergency Stop.............................................................. 48
4 User Maintenance
4.1
4.2
4.3
4.4
4.4.1
4.4.2
4.4.3
4.5
4.5.1
4.5.2
4.5.3
4.6
4.6.1
4.7
4.7.1
4.8
4.8.1
4.9
4.9.1
4.9.2
4.9.3
4.9.4
4.10
4.11
System administration...................................................................... 49
User interface ........................................................................................ 50
User maintenance schedule .......................................................... 51
Cleaning procedures .......................................................................... 52
Instruments ............................................................................................ 52
Gel trays................................................................................................... 52
Microplate lids ....................................................................................... 52
Camera alignment .............................................................................. 53
Required accessories ......................................................................... 53
Focus and aperture adjustment .................................................. 53
Alignment procedure ......................................................................... 55
Changing the spotter needle ......................................................... 60
Changing procedure .......................................................................... 61
Changing the digester needles ..................................................... 68
Changing procedure .......................................................................... 68
Changing picker head ....................................................................... 73
Changing procedure .......................................................................... 73
Priming...................................................................................................... 77
Priming the Digester .......................................................................... 77
Priming the Picker ............................................................................... 78
Priming the Spotter............................................................................. 78
System priming .................................................................................... 79
Changing syringes .............................................................................. 80
Preventive maintenance .................................................................. 82
5 Troubleshooting
6 Specifications
6.1
Technical specifications ................................................................... 85
6.2
Performance specification .............................................................. 86
6.3
Regulatory requirements ................................................................. 86
6.4
Ordering information ......................................................................... 87
6.4.1 Accessories ............................................................................................. 87
6.4.2 Related products.................................................................................. 89
Index ............................................................................................................................. 91
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List of figures
List of figures
Fig 1-1.
Fig 1-2.
Fig 1-3.
Fig 2-1.
Fig 2-2.
Fig 2-3.
Fig 2-4.
Fig 2-5.
Fig 2-6.
Fig 2-7.
Fig 2-8.
Fig 2-9.
Fig 2-10.
Fig 2-11.
Fig 2-12.
Fig 2-13.
Fig 2-14.
Fig 2-15.
Fig 2-16.
Fig 2-17.
Fig 2-18.
Fig 2-19.
Fig 2-20.
Fig 2-21.
Fig 2-22.
Fig 2-23.
Fig 2-24.
Fig 3-1.
Fig 3-2.
Fig 4-1.
Fig 4-2.
Fig 4-3.
Fig 4-4.
Fig 4-5.
Ettan Spot Handling Workstation ............................................... 11
Ettan Spot Handling Workstation configuration ................. 12
Rating label layout ............................................................................ 13
Ettan Spot Handling Workstation ............................................... 17
Reagent/waste bottles and syringe pump module ........... 18
The cabinet’s lower left compartment ..................................... 19
The cabinet’s lower right compartment .................................. 20
Front panel controls and indicators .......................................... 22
Mains connections and ventilation outlet .............................. 23
Safety switch on the cabinet door ............................................ 23
The Spot Picker & Spotter ............................................................... 24
The Syringe pump module ............................................................ 25
The picker head ................................................................................... 26
Spring action when lowering the picker head ..................... 26
The video camera .............................................................................. 27
Spot picker and spotter heads ..................................................... 28
Spotting onto a MALDI target ....................................................... 28
The Digester .......................................................................................... 29
The 8-needle multiple probe head ............................................. 30
The enzyme cooler ............................................................................ 30
The dryer ................................................................................................ 31
The ion generator ............................................................................... 32
The incubator ....................................................................................... 33
Microplate lid. ....................................................................................... 34
The transfer robot .............................................................................. 35
The hotel ................................................................................................. 36
Barcode reader .................................................................................... 37
Ettan Spot Handling Workstation front panel ...................... 45
The Emergency Stop button on the cabinet´s front panel 47
The System Configuration window ........................................... 50
Accessories for camera alignment ............................................ 53
Calibration plate 18-1156-66 ....................................................... 60
The picker head ................................................................................... 73
Final appearance of the assembled picker head ............... 75
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List of figures
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Introduction 1
1
Introduction
IMPORTANT! Ettan™ Spot Handling Workstation is intended for research
use only and should not be used in any clinical procedures for diagnostic
purposes.
Ettan Spot Handling Workstation is a fully integrated instrument for
automatic processing and transfer of biomolecules from polyacrylamide
electrophoresis gels to targets used in MALDI-ToF mass spectrometer (MS).
Processing is done in three steps:
1
Spot picking from 2-D electrophoresis gels.
This step is defined as removing material of interest from polyacrylamide
electrophoresis gels (contained in gel plugs) and dispensing it into
microplate wells.
2
Digestion of the picked proteins.
This step is defined as pre-treatment (washing, destaining, etc.),
enzymatic cleaving and extracting biomolecules from the gel plugs.
Digestion comprises a number of substeps, including dispensing into
microplate wells and drying, both as an intermediate and a last step.
3
Spotting (dispensing) of samples onto targets for the MS.
This step is defined as pre-treatment (dissolving, etc.) and transfer of
extracted biomolecules and matrix solution from microplate wells or
containers to MALDI targets.
1.1
The System Manual
This manual provides technical information, process descriptions and startup/shutdown instructions for Ettan Spot Handling Workstation. In addition,
maintenance schedules and instructions for user maintenance are included.
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9
1 Introduction
1.2
System overview
Ettan Spot Handling Workstation consists of the following sub-systems
(see Fig. 1-1):
10
•
Cabinet
•
Spot Picker & Spotter
•
Digester with dryer and incubator
•
Transfer robot
•
Gel hotel
•
Microplate/MS sample tray hotel
•
Ettan Spot Handling Workstation control software running under
Windows 2000® operating system on a PC
•
PC with frame grabber card for video display
Ettan Spot Handling Workstation System Manual 18-1153-54 Edition AD
Introduction 1
Transfer robot
Dryer (hidden)
Digester
Incubator (hidden)
Reagent bottles
Waste bottles
Cabinet
Spot Picker & Spotter
Gel hotel
Microplate/MS sample tray hotel
Fig 1-1. Ettan Spot Handling Workstation.
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1 Introduction
Control Panel
Ventilation Fan
Syringe Pump
Camera to GRABBER
Gilson 215
Gilson 215 Multi Probe
CCD Camera
Power
Multi Valve
Door Interlock
Run
Error
Interlock
Stop
GSIOC
Enzyme Cooler
Reset
Barcode
Reader
Mains
Robot
Temp- & I/O
Dryer
System
Computer
Incubator
Controller and Power Supply
for Robot
RS232
Ethernet
RS232
Barcode
Reader
RS232
Mouse
Fig 1-2. Ettan Spot Handling Workstation configuration.
The sub-systems are described in detail in the System description chapter of
this manual, where brief descriptions of the individual components are also
given. Ettan Spot Handling Workstation control software is described in the
separate Ettan Spot Handling Workstation User Manual and Online Help.
The package contents are described in Section 1.3.
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Introduction 1
1.2.1
Rating label
The rating label is located at the right side of cabinet close to the mains
connections. The layout of rating label, see figure below.
Fig 1-3. Rating label layout.
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1 Introduction
1.3
Package contents
Ettan Spot Handling Workstation is delivered with the products listed in
Table 1-1. For ordering information, refer to Section 6.4.
Table 1-1. Ettan Spot Handling Workstation, delivered products.
Product
14
Quantity
Ettan Spot Handling Workstation control software
1
Picker head 1.4 mm for 1.0 mm thick gels
2
Picker head 2.0 mm for 1.0 mm thick gels
2
Reference markers, sheet incl. 560 pieces
2
Gel tray
12
Gel holder
24
Glass lid for gel tray
12
Tubing, Spot Picker & Spotter
1
Tubing, Digester
1
Tubing, waste
1
Camera calibration foot
5
Calibration plate
1
Waste bottle, 15 liter
2
Reagent bottle, 5 liter
3
Reagent bottle, 2 liter
3
Reagent bottle, 1 liter
4
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Installation of ÄKTAcrossflow 10
1.4
Accessories
In addition to the items delivered with the system, Ettan Spot Handling
Workstation requires the following accessories:
•
Image scanner: ImageScanner™, or Typhoon™*
•
Gel image evaluation software, e.g. ImageMaster™ (Nonlinear Dynamics)
See Chapter 6 for specifications and ordering information.
* If you want to use other scanners, please contact GE Healthcare.
1.5
Associated documentation
1.5.1
Ettan Spot Handling Workstation Safety Handbook
Provides the safety instructions that must be strictly followed for the safe
use of Ettan Spot Handling Workstation.
1.5.2
Ettan Spot Handling Workstation Site Preparation Guide
Provides the data needed to prepare the laboratory for the installation of
Ettan Spot Handling Workstation.
1.5.3
Ettan Spot Handling Workstation User Manual
Contains detailed operating instructions.
1.5.4
Ettan Spot Handling Workstation Online Help
An online help function can be reached from the software. A troubleshooting
guide is also included in this function.
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1 Introduction
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System description 2
2
System description
2.1
General
Fig 2-1. Ettan Spot Handling Workstation.
Ettan Spot Handling Workstation consists of fully integrated instruments for
the automatic processing and transfer of biomolecules from polyacrylamide
electrophoresis gels to MALDI-ToF MS targets. To control the system, a
personal computer running Ettan Spot Handling Workstation control
software is included.
Communication between the computer and the various modules and
components of Ettan Spot Handling Workstation is achieved via high speed
data network connections.
Reagent bottles for the fluid handling equipment and the syringe pump
module are placed on shelves to the left of the cabinet. The waste
bottles are placed on the bottom shelf. The bottles are connected to the
instruments via tubing through the cabinet wall, see Fig. 2-2.
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2 System description
Reagent bottles for the
Digester
Syringe pump module
Reagent bottles for the Spot
Picker & Spotter
Waste bottles
Fig 2-2. Reagent/waste bottles and syringe pump module.
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System description 2
2.2
The Cabinet
The purpose of the cabinet is to house all mechanics, for operator and
personnel safety, to protect personnel from hazardous chemicals, and to
avoid contamination of samples in process. The operator has access to all
components via the cabinet doors.
The cabinet consists of two main parts:
1
The left part contains (see Fig. 2-3):
•
Digester and Spot Picker & Spotter (upper compartment)
•
computer and temperature-I/O controller (lower compartment)
2
The right part contains (see Fig. 2-4):
•
robot, dryer, incubator and hotel for gel trays, microplates and
MS sample trays (upper compartment)
•
power supply and control equipment for the robot (placed on a sliding
shelf in the lower compartment)
The cabinet is equipped with an evacuation fan for transporting process air
to the ventilation system. Since most of the evacuated air will contain fumes
that are heavier than air, the inlet to the evacuation fan is located at a low
level. In addition, the cabinet has filters for the incoming air to minimize
contaminants in the cabinet.
TemperatureI/0 controller
Computer
Fig 2-3. The cabinet’s lower left compartment.
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2 System description
The robot´s
power supply
The robot´s
control
equipment
Fig 2-4. The cabinet’s lower right compartment.
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System description 2
2.2.1
Front panel controls and indicators
The layout of the Ettan Spot Handling Workstation front panel is shown in
Fig. 2-5.
The front panel has two control buttons:
Emergency Stop When activating this red push-button, the workstation´s
instruments and the transfer robot immediately cease
movement and all motors are de-energized.
WARNING! If the Picker/Spotter or the Digester is in its homing procedure
when the emergency stop button is activated, the homing will be
completed before the instrument ceases movement. Homing means that
the unit is seeking its zero position. The text “HOMING” is shown in the
unit´s display.
Reset
This non-latching push-button is used to acknowledge
closed cabinet doors. The push-button´s yellow indicator
is on when one or several cabinet doors are opened, and
will go off after acknowledgement.
Power
This blue indicator is on when the mains power is
switched on, i.e. when power is present in the workstation.
Running
This blue indicator is on during batch processing, and is
flashing when a batch process is in Pause Batch mode.
The indicator is off when no batch is present in the
workstation.
Error
This red indicator is on if an error occurs, and is flashing
when a warning appears in the Handle Events dialog. To
acknowledge the warning, select the warnings row, and
click Clear warning. The error indicator will stop flashing.
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2 System description
Power indicator (blue)
Running indicator (blue)
Error indicator (red)
Emergency Stop push-button (red)
Reset push-button (yellow)
Fig 2-5. Front panel controls and indicators.
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System description 2
2.2.2
External controls and connections
The controls and connections located at the right side of the cabinet are
shown in Fig. 2-6.
Mains power switch
Mains power inlet
Ventilation outlet
Fig 2-6. Mains connections and ventilation outlet.
The following connections are located at the left side of the cabinet, see
Fig. 2-2.
1
Reagent bottle tubing.
2
Waste bottle tubing.
3
Syringe pump module tubing.
2.2.3
Safety switches
The upper doors of the cabinet are equipped with safety switches. The
switches are mounted at the same level as the lower part of the doors.
Cabinet door
Safety switch
Fig 2-7. Safety switch on the cabinet door.
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2 System description
2.3
The Spot Picker & Spotter
The Spot Picker & Spotter is a robotic system that can move the picker/
spotter head in three directions: X, Y and Z. The syringe pump (see Fig. 29) allows precise handling of liquid and picked gel plugs. The valve next to
the syringe pump controls the flow from the liquid supply, and to/from the
picker/spotter head.
The picker head and the spotter head are mounted on the same robot arm.
This is possible since they are not used at the same time.
The picker head movements are guided by a camera system. The camera,
which is attached to the Z-arm, is connected to the PC via a frame grabber
card. Reference markers on the gel are identified using the camera. This
enables the pick list coordinates from the scanned gel to be transferred to
picker head positions during automated picking.
Two microplate supports and a vial holder for matrix solutions and
calibrants are placed beside the gel tray.
Z-arm
X-arm
Rinse station for
Spot Picker
Waste tube
Rinse station
for Spotter
Video camera
Y-arm
Spot Picker head
Gel tray
Spotter head
Gel tray support (4 pcs)
Microplate support (2 pcs)
Vial holder
Fig 2-8. The Spot Picker & Spotter.
Note: Fig. 2-8 shows a previous spotter head design.
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System description 2
Valve
Syringe pump for Spot Picker
Syringe pump for Spotter
Fig 2-9. The Syringe pump module.
The syringe pump module is placed on a shelf to the left of the cabinet, see
Fig. 2-2.
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2 System description
2.3.1
The picker head
The picker head is mounted on the Z-arm just behind the video camera.
Tube fitting
Piston
Retaining
spring
Punch
Fig 2-10. The picker head.
The retaining spring creates a pressure against the gel backing during
picking, thus ensuring that the gel plug will be properly excised. When the
Z-height is correctly adjusted, the picker head should be lowered until a
gap is formed between the piston and the body of the picker head, thereby
allowing the spring to press against the gel backing.
Fig 2-11. Spring action when lowering the picker head.
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System description 2
2.3.2
Video camera
The video camera is used to establish the coordinates for the gel to be
picked. By detecting the reference markers on the gel and comparing with
the coordinates in the pick list, the picking coordinates can be determined.
Since the camera lens is located at a distance from the picker head, its
position relative to the picker head has to be precisely defined to ensure
high precision picking. The camera alignment routine provides the means
for this, see Chapter 4. Also, instructions for adjustment of camera focus and
aperture are provided in Chapter 4.
Aperture setting
Focus setting
Fig 2-12. The video camera.
The video signal is processed by a frame grabber card in the PC before
displaying the image in Ettan Spot Handling Workstation control software.
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2 System description
2.3.3
The spotter head
The spotter head is mounted on a bracket on the same Z-arm as the spot
picker head.
Spotter head
Spotter needle
Spot Picker head
Fig 2-13. Spot picker and spotter heads.
Spotter needle
Rinse station
MALDI target
MS sample tray
96-well microplate
Vial holder
Fig 2-14. Spotting onto a MALDI target.
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System description 2
2.4
The digestion system
The Digester is a robotic system designed to prepare gel plugs for enzymatic
digestion of proteins and subsequent extraction of peptides. The instrument
has an 8-needle multiple probe head that can move in the X, Y and Z
directions.
The multiple probe head is connected to an 8-channel multi-probe dilutor.
An 8-position valve actuator with a common outlet is used to select the
different liquid solvents.
The rinse station has two positions for rinsing; one for shallow rinsing of the
needles and one for deep rinsing.
Also included in the digestion system are a dryer and an incubator, see
descriptions below.
Valve actuator
Rinse station
VALVEMATE™
REMOTE
MODE
Multi-probe dilutor
Multiple probe head
Enzyme cooler
Enzyme container
Microplate
Fig 2-15. The Digester.
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2 System description
2.4.1
The multiple probe head
The 8-needle multiple probe head is mounted on the Z-arm.
Z-arm
Multiple probe head
Digester needles
Fig 2-16. The 8-needle multiple probe head.
Enzyme container
Enzyme cooler
Fig 2-17. The enzyme cooler.
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System description 2
2.4.2
The dryer
The purpose of the dryer is to evaporate the liquid/gel plug in the microplate
wells and to dry peptide extract. The dryer consists of open chambers with
space for six microplates. Each microplate position has a heater located
underneath.
The dryer is also equipped with fans for air circulation. An air distributor
is placed above the microplate position to direct the air flow into each
microplate well. An evacuation fan in the cabinet then transports the air to
the ventilation system.
Note: The upper two microplate positions have no air circulation.
Microplate position
Heater
Air distributor
Fig 2-18. The dryer.
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2 System description
2.4.3
The ion generator
The purpose of the ion generator is to neutralize the electrostatic charged
air surrounding the microplates when placed in the dryer, and thus minimize
the electrostatic influence on the gel plugs.
The ion generator is mounted in a separate box attached to the upper part
of the dryer, see figure below.
Ion generator box
Dryer
Fig 2-19. The ion generator.
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System description 2
2.4.4
The incubator
The purpose of the incubator is to establish a constant temperature during
enzymatic digestion at a predefined time. The incubator has one closed
chamber with space for twelve microplates and is equipped with heating
control.
During incubation, the microplates are covered with separate lids (see
Fig. 2-21) to avoid evaporation. The lid is located above each microplate
position, and is placed onto the microplate by the robot when it inserts the
microplate into the incubator.
Microplate lid (12 pcs)
Fig 2-20. The incubator.
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2 System description
Fig 2-21. Microplate lid.
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System description 2
2.5
The transfer robot
A robot system with six degrees of freedom is used as a bridge between the
different modules in Ettan Spot Handling Workstation.
The robot handles gel trays, microplates and MS sample trays as well as
opens and closes the incubator door. In addition, the robot can handle the
lids for both gel trays and microplates.
The robot is equipped with a servo gripper for handling gel trays as well as
microplates and MS sample trays.
Servo gripper
Fig 2-22. The transfer robot.
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2 System description
2.6
The hotel
The hotel is divided into two sections; one containing shelves for microplates
and MS sample trays, and one with rails for gel trays.
MS sample tray
Barcode reader
Gel tray
Microplate
Microplate/MS sample tray hotel
Gel hotel
Fig 2-23. The hotel.
2.6.1
Gel hotel
The gel hotel has space for twelve gel trays. The bottom position of the hotel
is used to lift off the glass lid from the gel tray. When in this position, the
robot lowers the gel tray approx. 10 mm and the glass lid remains on the
shelves for temporary storage.
Note: The gel tray has a silicone sealing on the top edge. This protects the
gel during storage.
2.6.2
Microplate/MS sample tray hotel
The Microplate/MS sample tray hotel can house 24 microplates and 12 MS
sample trays for a batch run.
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System description 2
2.6.3
Barcode reader
WARNING! LASER RADIATION. DO NOT STARE INTO BEAM OR VIEW
DIRECTLY WITH OPTICAL INSTRUMENT. CLASS 2 LASER PRODUCT.
Do not open cover to the internal barcode reader. Refer servicing to
qualified service personnel.
The barcode reader, located at the top of the hotel, is used to track
samples from gel to microplate to MALDI targets. After moving a gel tray, a
microplate or a MS sample tray from the hotel, the transfer robot will stop at
the reading position in front of the barcode reader.
Fig 2-24. Barcode reader.
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2 System description
2.7
Workflow in Ettan Spot Handling Workstation
This section gives a brief step-by-step description of the workflow through
Ettan Workstation. For detailed description, refer to Ettan Spot Handling
Workstation User Manual.
2.7.1
The workstation´s control software starts to identify all gel trays,
microplates and MS sample trays.
2
The transfer robot moves the gel tray to the bottom position of the hotel
to lift off the glass lid.
3
The robot moves the gel tray to the Spot Picker & Spotter.
4
The robot picks up a microplate from the hotel and moves it to the Spot
Picker & Spotter.
2.7.2
38
Batch run start
1
Spot picking
1
The Spot Picker locates the reference markers.
2
The picker head moves to the rinse station.
3
The picker head moves to a gel spot according to pick list coordinates.
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System description 2
4
The gel spot is released from the gel backing (under liquid) by punching
and sideways movements (“jazz”).
5
The gel plug is aspirated using Milli-Q™ water as hydraulic liquid.
6
The gel plug is dispensed into a microplate well together with Milli-Q
water or destaining buffer.
7
The picker head moves to the rinse station for washing using
Milli-Q water (optional).
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2 System description
2.7.3
40
Digestion
1
The robot moves the finished microplate with gel plugs and picker liquid
up to the Digester.
2
The Digester aspirates the picker liquid from the gel plug wells and
moves it to the rinse station.
3
The Digester destains and equilibrates the gel plugs remaining (prior to
enzyme addition and digestion) by one, or several washing procedure(s).
4
The robot moves the finished microplate to the dryer for dehydration of
the gel plugs under a predetermined time (to enhance enzyme entry into
the gel plug before digestion).
5
The robot moves the microplate back to the Digester.
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System description 2
6
The multi-probe head moves to the enzyme container.
7
The Digester adds enzyme (e.g. Trypsin) which enters into and
rehydrates the gel plugs.
8
The Digester adds buffer (if needed).
9
The multiple probe head moves to the rinse station.
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2 System description
10 The robot moves the finished microplate to the incubator for enzymatic
digestion under a predetermined time. During incubation, the microplate
is covered with a lid to avoid evaporation.
11 The robot moves the microplate from the incubator back to the Digester
(position in front of the enzyme container).
12 The robot moves a new, empty microplate from the hotel to the Digester
(position in front of the rinse station).
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System description 2
13 The Digester moves the digested samples (extracted from the gel) to the
empty microplate.
14 The robot moves the finished microplate to the dryer for drying under a
predetermined time.
15 The robot moves the finished microplate to the Spot Picker & Spotter.
16 The robot moves an MS sample tray from the hotel to the position beside
the microplate.
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2.7.4
Spotting (standard method)
1
The Spotter dissolves the sample in the microplate by using Matrix
solution.
2
The Spotter mixes the sample in the microplate well by a defined
number of aspirating/dispensing cycles.
3
The Spotter transfers the sample to the MALDI target.
4
The sample is left on the MALDI target for air drying.
5
The spotter needle moves to the rinse station for rinsing in spotter liquid.
The workstation continues the processing to complete the required batch
run.
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Start-up and shutdown 3
3
Start-up and shutdown
This chapter provides start-up and shutdown instructions for Ettan Spot
Handling Workstation. Refer to Ettan Spot Handling Workstation User Manual
for instructions on the consumables needed for a batch run.
3.1
Starting the workstation
To start Ettan Spot Handling Workstation:
1
Turn on mains power to Ettan Spot Handling Workstation with the mains
power switch at the right side of the cabinet, see Fig. 2-6.
2
Check that Ettan Spot Handling Workstation is operating:
•
the Power indicator should light
•
the PC should show the log-on screen
Power indicator (blue)
Running indicator (blue)
Error indicator (red)
Emergency Stop push-button (red)
Reset push-button (yellow)
Fig 3-1. Ettan Spot Handling Workstation front panel.
3
Log-in to Windows 2000.
Further operating instructions are provided in Ettan Spot Handling
Workstation User Manual.
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3 Start up and shutdown
3.2
Before batch start-up
Before starting a batch run, the following checks should be done:
CAUTION! Always make sure that no foreign objects are left inside the
cabinet.
1
Check that the correct spot picker head for the actual batch run is
selected. For instructions on changing spot picker head, see Chapter 4
User Maintenance of this manual.
2
Check that there is not any calibration plate in the Spot Picker & Spotter
and the Digester.
3
Check that there is not any microplate in the Spot Picker & Spotter, the
Digester, the incubator and the dryer.
4
Check that there is not any gel tray glass lid in the lower part of the gel
hotel.
5
Check that all microplates and MS sample trays are placed into the
center position in the frame of the hotel shelf.
6
Check that tubing and couplings are connected and tightened.
7
Check that the waste bottles are empty.
Further operating instructions are provided in Ettan Spot Handling
Workstation User Manual.
3.3
Pause batch mode
The pause batch mode can be used when a temporary stop is needed, e.g.
to remove a foreign object. Proceed as follows:
1
In the Batch Processing dialog, click the Pause button.
2
Wait until the Running indicator on the cabinet’s front panel flashes.
3
Open the cabinet door(s) and remove the foreign object.
4
Close the cabinet door(s).
5
Press the Reset button on the cabinet’s front panel.
Further operating instructions are provided in Ettan Spot Handling
Workstation User Manual.
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Start up and shutdown 3
3.4
Shutting down the workstation
When not in use, shut down the workstation:
1
In the menubar, choose command File:Exit.
2
Log-out from Windows 2000.
3
Switch off mains power with the mains power switch at the right side of
the cabinet.
3.5
Emergency shutdown
In the event of an emergency, press the Emergency Stop button on the
front panel of Ettan Spot Handling Workstation, see Fig. 3-2 below. When
pressing the Emergency Stop button or if a cabinet door is opened during a
run, the workstation´s instruments and the transfer robot immediately cease
movement and all motors are de-energized.
WARNING! If the Picker/Spotter or the Digester is in its homing procedure
when the emergency stop button is activated, the homing will be
completed before the instrument ceases movement. Homing means that
the unit is seeking its zero position. The text “HOMING” is shown in the
unit´s display.
Emergency Stop push-button (red)
Fig 3-2. The Emergency Stop button on the cabinet´s front panel.
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3 Start up and shutdown
3.5.1
Reset the Emergency Stop
IMPORTANT! Do not switch-off mains power. When mains power is
switched-on after a switch-off, the transfer robot cannot perform
homing. Call GE Healthcare Service.
For information how to reset the emergency stop, re-start or abort a batch
run, refer to Ettan Spot Handling Workstation User Manual.
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User Maintenance 4
4
User Maintenance
User maintenance is important for safe and trouble-free operation of Ettan
Spot Handling Workstation. Preventive maintenance should be performed
on a yearly basis by qualified service personnel.
This chapter provides instructions for user maintenance and service
operations.
WARNING! MOVING PARTS. Do not read any barcodes with the external
barcode reader while performing user maintenance inside the cabinet.
Output signals from the barcode reader will be interpreted by the Spot
Picker & Spotter and Digester units as a command to move to their home
positions.
WARNING! NO SERVICEABLE PARTS INSIDE. Do not open covers. Service
and preventive maintenance should be performed by qualified personnel.
Contact your GE Healthcare representative for more service information.
4.1
System administration
A competent person at the laboratory should be trained and appointed
as system administrator for Ettan Spot Handling Workstation. The system
administrator should be responsible for the results and data backup.
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4 User Maintenance
4.2
User interface
Parts of the user maintenance are handled from the System Configuration
window, see Fig. 4-1 below.
Fig 4-1. The System Configuration window.
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User Maintenance 4
4.3
User maintenance schedule
Table 4-1 lists the maintenance operations that should be performed by the
user at regular intervals.
Table 4-1. User maintenance.
Interval
Action
Instructions/reference
Daily
Check the transfer robot’s
working area
Due to the risk of collision,
check that no foreign
objects are left in the
cabinet
When required
Clean the workstation
Wipe the cabinet and the
instrument covers with a
soft damp tissue. Let dry
completely before use. See
Section 4.4
Clean the microplate lids
See Section 4.4.3
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4.4
Cleaning procedures
4.4.1
Instruments
Cleaning the instruments regularly
For proper function, the instruments should be kept clean and dry. Chemical
stains and dust should be removed.
1
Wipe the instrument covers with a soft damp tissue. If needed, use a
mild detergent to remove stains.
2
Let the instrument dry completely before use.
3
Rinse the buffer lines with Milli-Q water or equivalent, using the prime
syringe tool.
4.4.2
Gel trays
When a batch run is completed:
1
Remove the gel from the gel tray and store/discard as appropriate.
2
If the gel is to be discarded, scrape the gel off the glass plate with a
plastic spacer or similar object. Then put the plates into a 5% Decon™
solution (or equivalent) overnight to remove any gel fragments which
adhere to the plate.
3
Rinse the liquid out of the gel tray with Milli-Q water or equivalent and
leave the gel tray to dry.
4.4.3
52
Microplate lids
1
Wipe the lid with a soft damp tissue. If needed, use a mild detergent.
2
Rinse the lid with Milli-Q water or equivalent and let dry.
3
Carefully insert the lids into the incubator.
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4.5
Camera alignment
WARNING! MOVING PARTS. Do not read any barcodes with the external
barcode reader while performing user maintenance inside the cabinet.
Output signals from the barcode reader will be interpreted by the Spot
Picker & Spotter and Digester units as a command to move to their home
positions.
4.5.1
Required accessories
Following accessories are needed for the camera alignment:
•
Gel tray
•
Camera alignment foot, code no. 18-1143-33
•
White paper
•
Double-sided adhesive tape
Alignment position
Camera alignment
foot
Gel tray
Gel tray support
Fig 4-2. Accessories for camera alignment.
4.5.2
Focus and aperture adjustment
Before starting the camera alignment, check that the camera lens is
focused on the bottom of the gel tray:
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54
1
From the menubar, select System:System Configuration.
2
Click on System Service.
3
Click on Start. The Service Utilities dialog appears.
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User Maintenance 4
4
Click on View Camera Image. This will open a camera window on the
screen.
5
Place the gel tray onto its support in the Picker/Spotter.
6
Place a piece of white paper (size approx. 100 × 100 mm) with printed
black text into the corner of the tray beneath the camera. Ensure that
the paper lays flat on the bottom of the tray.
7
If necessary, adjust the aperture to normal brightness by using the
aperture setting ring, see figure below.
Aperture setting ring
Focus setting ring
8
Adjust the focus to a sharp picture by using the focus setting ring, see
figure above.
9
IMPORTANT! Set the aperture to 4 (four).
10 Remove the gel tray.
11 Perform the camera alignment procedure, see Section 4.5.3 below.
4.5.3
Alignment procedure
CAUTION! Before starting camera alignment, make sure that the Zarm is
properly mounted as high as possible on the Y-arm.
1
Prepare the gel tray as follows, see Fig. 4-2:
•
Place a piece of white paper in the center of the gel tray using adhesive
tape.
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4 User Maintenance
•
Place a strip of double-sided adhesive tape onto the white paper across
the alignment position in the center of the gel tray.
•
Place the gel tray onto the gel tray support in the Picker/Spotter.
2
From the menubar, select System:System Configuration.
3
Click on the Camera Alignment tab.
WARNING! MOVING PARTS. Do not enter the Spot Picker & Spotter´s
working area directly after clicking the camera alignment tab since the
picker/spotter head and camera assembly can make sudden, rapid
movements. Wait at least 30 seconds before applying the camera
alignment foot.
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4
Apply the calibration alignment foot onto the picker head.
5
Close the cabinet door.
6
Press Reset on the cabinet´s front panel.
7
Check the Confirm that the marker is placed on the pick head box.
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4 User Maintenance
8
Click the Start button.
WARNING! MOVING PARTS. Do not enter the Spot Picker & Spotter´s
working area directly after clicking the Start button since the picker/
spotter head and camera assembly can make sudden, rapid movements.
The status bar is yellow during processing and turns into green when the
camera alignment is completed.
9
58
Open the cabinet door.
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User Maintenance 4
10 Repeat steps 4 to 8 until all status bars are green.
11 Click the Save button to save the alignment values.
12 Remove the gel tray.
13 Close the cabinet door.
14 Press Reset on the cabinet’s front panel.
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4 User maintenance
4.6
Changing the spotter needle
WARNING! MOVING PARTS. Do not read any barcodes with the external
barcode reader while performing user maintenance inside the cabinet.
Output signals from the barcode reader will be interpreted by the Spot
Picker & Spotter and Digester units as a command to move to their home
positions.
Note: Calibration plate, code no. 18-1156-66, is needed for the changing
procedure.
Digester needles
knobs (8 pos.)
Picker head
knob (1 pos.)
Spotter needle
knob (1 pos.)
Fig 4-3. Calibration plate 18-1156-66.
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4.6.1
1
Changing procedure
From the menubar, select System:System Configuration.
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62
2
Click on System Service.
3
Click on Start.
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User Maintenance 4
The Service Utilities dialog appears.
4
In the Service Utilities dialog, click on Change Spotter Needle.
5
Open the cabinet door.
WARNING! MOVING PARTS. Do not enter the Spot Picker & Spotter´s
working area directly after opening the cabinet door(s) since the picker/
spotter head can make sudden, rapid movements. Wait at least 30
seconds before entering this working area.
6
Place the calibration plate onto plate position 1.
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4 User Maintenance
7
In the Spotter Needle Change dialog, click on Next.
The spotter needle moves to a position 1.0 mm above and 50 mm to the
right of the calibration position.
Note: The Spotter Needle Change dialog shows a picture of the previous
spotter head design.
8
Replace the spotter needle as follows:
•
Loosen the four needle holder screws with an Allen key, see figure
below. Do not remove the needle holder.
Tubing
Upper retaining nut
Connector
Lower retaining nut
Needle holder
Screw (4 pcs)
Spotter needle
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•
Lift out the needle with its tubing and connector from the needle
holder, see figure below.
Connector
Spotter needle
Low retaining nut
•
Loosen the lower retaining nut. Use a wrench to hold the connector
while loosen the nut. Do not remove the retaining nut.
•
Remove the worn needle.
•
Carefully insert the new needle. Press the needle against its
innermost position.
•
Fingertighten the retaining nut.
•
Carefully insert the needle with its tubing and connector into the
needle holder.
•
Tighten the four screws while holding the tip of the needle approx.
1 mm above the calibration plate. Be carefully not to scratch the
needle against the calibration plate.
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9
Click on Next.
The spotter needle moves to the calibration position.
10 Adjust the XYZ-positions against the calibration plate using the Nudge
Needle position buttons as follows:
•
Carefully lower the needle towards the calibration knob. Stop
lowering when the needle is approx. 0.5 mm above the calibration
knob.
•
Adjust the XY-position against the center of the calibration knob. The
tip of the needle must point precisely to the center of the calibration
knob.
Press the Check Position button to verify the adjustment. The picker/
spotter head moves to the home position and then back to its new
XY-position. If necessary, re-calibrate the XY-position.
•
Adjust the Z-position so that a strip of thin aluminum foil is barely
stuck between the needle and the knob. Do as follows:
1. Carefully lower the needle (0.1 mm steps) while moving slightly the
aluminum foil sideways. Stop lowering when the foil is stuck.
2. Press the Check Position button to verify the adjustment. The foil
should stick.
3. Raise the needle 0.1 mm and press the Check Position button to
verify. The aluminum foil should not stick.
4. Lower the needle 0.1 mm and press the Check Position button to
verify that the aluminum foil now sticks. Re-calibrate if necessary.
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•
Check that the tip of the needle points precisely to the center of the
calibration knob, see figure above (item 4.6.1.9).
•
If necessary, re-calibrate the XY-position.
11 Click on Next.
12 Click on Finish to save the changed position values.
13 Remove the calibration plate.
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4.7
Changing the digester needles
WARNING! MOVING PARTS. Do not read any barcodes with the external
barcode reader while performing user maintenance inside the cabinet.
Output signals from the barcode reader will be interpreted by the Spot
Picker & Spotter and Digester units as a command to move to their home
positions.
Note: Calibration plate, code no. 18-1156-66, is needed for the changing
procedure, see Fig. 4-3.
4.7.1
Changing procedure
1
From the menubar, select System:System Configuration.
2
Click on System Service, then click Start.
3
In the Service Utilities dialog, click on Change Digester Needles.
4
Open the cabinet door.
WARNING! MOVING PARTS. Do not enter the Digester´s working area
directly after opening the cabinet door(s) since the digester head can
make sudden, rapid movements. Wait at least 30 seconds before
entering this working area.
5
68
Place the calibration plate onto plate position 1.
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User Maintenance 4
6
In the Digester Needle Change dialog, click on Next.
The digester needles move to a position 1.0 mm above and 50 mm to the
right of the calibration position.
7
Replace the digester needles as follows:
•
Loosen and remove the retaining nuts with its tubing on top of the
needle holder
Tubing with tubing end ferrules
Retaining nut
Needle holder
Digester needles
Needle guide
Note: Be careful not to lose the tubing end ferrules.
•
Remove the worn needles
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4 User Maintenance
•
Carefully insert the new needles
•
Let the needles drop down through the holes in the needle guide
•
Attach the retaining nuts with its tubing
•
Finger tighten the retaining nuts
•
Loosen the stop screw with an Allen key
Stop screw
Allen key
8
Click on Next.
9
Check that the needles are flush with the calibration plate, i.e. the
needles should be resting on the calibration plate. Adjust if necessary.
10 Tighten the stop screws while holding the needles slightly against the
calibration plate.
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11 Check that the needles are flush with the calibration plate. If not, repeat
the procedure.
12 Click on Next.
The digester needles move to a position 1.0 mm above the calibration
position.
13 Adjust the XY-position of the needles against the calibration plate using
the Nudge Needle position buttons. The tip of the needles must be
clearly within the area of the calibration knobs.
14 Press the Check Position button to verify the adjustments. The digester
needles move to the home position and then back to the new
XY-position.
15 Visually check the needle positions. If necessary, re-calibrate
XY-positions.
16 Click on Next.
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17 Click on Finish to save the changed position.
18 Remove the calibration plate.
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4.8
Changing picker head
WARNING! MOVING PARTS. Do not read any barcodes with the external
barcode reader while performing user maintenance inside the cabinet.
Output signals from the barcode reader will be interpreted by the Spot
Picker & Spotter and Digester units as a command to move to their home
positions.
The Spot Picker can be used with two sizes of picker heads (1.4 and 2.0
mm). The picker heads are easily changed by a simple screw-in/screwout method. After changing a picker head, a camera alignment should
be performed to establish the offset between the picker head and the
camera lens. The gel Z-position may also have to be adjusted in the System
Configuration Spot Picker window.
Retaining spring
O-ring
Punch
Fig 4-4. The picker head.
Note: Calibration plate, code no. 18-1156-66, is needed for the changing
procedure, see Fig. 4-3.
4.8.1
Changing procedure
1
From the menubar, select System:System Configuration.
2
Click on System Service, then click Start.
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3
In the Service Utilities dialog, click on Change Picker Head.
4
Open the cabinet door.
WARNING! MOVING PARTS. Do not enter the Spot Picker & Spotter´s
working area directly after opening the cabinet door(s) since the picker/
spotter head can make sudden, rapid movements. Wait at least
30 seconds before entering this working area.
5
Place the calibration plate onto plate position 1.
6
In the Picker Head Change dialog, click on Next.
The picker head moves to a position 100.0 mm to the left of the calibration
position.
7
Remove the picker head as follows:
•
74
Hold the top of the picking piston with
one hand, and with the other unscrew
the picker head from the base of the piston.
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User Maintenance 4
•
8
When the picker head is completely unscrewed from the base of the
piston, place it in a safe location until it is required once more. Make
sure that the retaining spring is also kept safe.
Attach the new picker head as follows:
•
Place the retaining spring over the screw thread
on the top of the picker head.
•
Screw the picker head onto the base of the piston,
making sure that the screw thread is inside the piston.
At the same time, hold the top of the piston steady with
your other hand.
•
Slowly screw the picker head into the piston
while holding the top of the piston still with
the other hand. Tighten the picker head
against the stop position.
Fig 4-5. Final appearance of the assembled picker head.
9
In the Picker Head Change dialog, click on Next
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The picker head moves to the calibration position.
10 Adjust the XYZ-position of the picker head against the calibration knob
using the Nudge Picker Head position buttons.
11 Press the Check Position button to verify the adjustment. The picker
head moves to the home position and then back to its new XYZposition.
12 Click on Next.
13 Click on Finish to save the changed position.
14 Remove the calibration plate.
Proceed by calibrating the distance between the picker head and the
camera. For instructions on camera alignment, see Section 4.5.
IMPORTANT! The new picker head must be registered in the “Picker Head
Management”, see Ettan Spot Handling Workstation User Manual.
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4.9
Priming
Priming the syringes removes air bubbles in the spot picker/spotter and
digester tubing, and also rinses the tubing with fresh solution. Priming is
performed as follows:
•
before starting spot picking, spotting and digestion processing, see
descriptions 4.9.1, 4.9.2, 4.9.3 and 4.9.4 below
•
automatically during spotting and digestion processing However,
additional priming should be performed:
•
after replacement of syringes
•
if the system will not be used for a longer period of time, and is to be
filled with distilled water
•
if the system is contaminated
Note: After priming, check that all tubing, outside and inside the
workstation, are filled with liquid and no air is visible.
Note: Priming can also be done from the Batch Processing window, refer to
Ettan Spot Handling Workstation User Manual.
4.9.1
Priming the Digester
1
Check that the tube fittings are properly tightened.
2
From the menubar, select System:System Configuration.
3
Click on System Service, then click Start.
4
In the Service Utilities dialog, click on System Priming.
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The System Priming Digester dialog appears.
5
Check the Enable Digester Priming box.
6
Enter recommended values and check the Valve Position boxes for the
valve positions which have connected liquids.
7
Click on Start.
8
The Digester moves to its rinse station.
9
When priming is finished, click on Exit.
4.9.2
1
Check that the tube fittings are properly tightened.
2
In the System Priming dialog, select Picker.
3
Check the Enable Picker Priming box.
4
Enter recommended values.
5
Click on Start.
6
The Spot Picker & Spotter moves to its rinse station.
7
When priming is finished, click on Exit.
4.9.3
1
78
Priming the Picker
Priming the Spotter
Check that the tube fittings are properly tightened.
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2
In the System Priming dialog, select Spotter.
3
Check the Enable Spotter Priming box.
4
Enter recommended values.
5
Click on Start.
6
The Spot Picker & Spotter moves to its rinse station.
7
When priming is finished, click on Exit.
4.9.4
System priming
Priming of the Digester, the Picker and the Spotter can also be done at the
same time. Do as follows:
1
Check the Enable Digester Priming, the Enable Picker Priming and the
Enable Spotter Priming boxes.
2
Enter recommended values.
3
Click on Start.
4 When priming is finished, click on Exit.
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79
4 User Maintenance
4.10
Changing syringes
WARNING! MOVING PARTS. Do not read any barcodes with the external
barcode reader while performing user maintenance inside the cabinet.
Output signals from the barcode reader will be interpreted by the Spot
Picker & Spotter and Digester units as a command to move to their home
positions.
1
From the menubar, select System:System Configuration.
2
Click on System Service, then click Start.
3
In the Service Utilities dialog, click on Change Syringes.
4
To enable replacement of syringe(s), click on Lower Digester Syringes,
Lower Picker Syringe or Lower Spotter Syringe respectively. The
following will happen:
•
The needle/needles/picker head move(s) to its rinse station
•
The syringe pump moves to its home position
•
The syringe(s) lowers/lower
Note: When replacing the spotter syringe, remember to mount the enclosed
sealing into the connection between the tubing and the new syringe.
5
80
Before replacement, the plunger of the new syringe should be wetted as
Ettan Spot Handling Workstation System Manual 18-1153-54 Edition AD
User Maintenance 4
follows:
•
Remove the plunger from the barrel
•
Dip the plunger tip into Milli-Q water, and re-insert the plunger into the
glass barrel
•
Work the plunger up and down 5-6 times, re-wetting the tip, if necessary
6
After replacement of syringe(s), click on Exit.
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81
4 User Maintenance
4.11
Preventive maintenance
GE Healthcare recommends preventive maintenance (PM) to be performed
by qualified service personnel during the yearly service visit. The extent of
the PM is to be agreed in the service contract.
Recycling
This symbol indicates that the waste of electrical and electronic equipment
must not be disposed as unsorted municipal waste and must be collected
separately. Please contact an authorized representative of the manufacturer
for information concerning the decommissioning of equipment.
82
Ettan Spot Handling Workstation System Manual 18-1153-54 Edition AD
Troubleshooting 5
5
Troubleshooting
For information on troubleshooting, refer to Ettan Spot Handling Workstation
User Manual and Online Help.
Recycling
This symbol indicates that the waste of electrical and electronic equipment
must not be disposed as unsorted municipal waste and must be collected
separately. Please contact an authorized representative of the manufacturer
for information concerning the decommissioning of equipment.
Ettan Spot Handling Workstation System Manual 18-1153-54 Edition AD
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5 Troubleshooting
84
Ettan Spot Handling Workstation System Manual 18-1153-54 Edition AD
Specifications 6
6
Specifications
For complete specifications of each component, refer to the individual
hardware documentation.
Relevant system specifications are listed below.
6.1
Technical specifications
Table 6-1. Ettan Spot Handling Workstation technical specifications.
Ettan Spot Handling Workstation
Mains voltages
100/120/230/240 V AC + 10-15%,
50/60 Hz ± 2 Hz
Operating temperature
18–30 °C (65–86 °F)
Dimensions
251.5 × 129 × 198.5 cm (W × D × H)
Total weight
645 kg
Power consumption
1600 VA
Sample source
Polyacrylamide gels cast on glass or
GelBond PAG film backing
Maximum gel size
276 × 212 mm
Minimum gel size
180 × 160 mm
Gel thickness compatibility
1 mm
Spot picker head diameter
1.4, 2.0 mm
Detection system
compatibility
Silver, Coomassie™, fluorescence
Barcode reader:
Max. output radiation
1.2 mW (223 us)
Emitted wavelength
670 nm
Max. beam divergence
60 degrees
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85
6 Specifications
6.2
Performance specification
Table 6-2. Ettan Spot Handling Workstation performance specification.
Ettan Spot Handling Workstation
Process capacity
6.3
12 Ettan DALT II gels and 1152
protein spots per batch in approx.
30 hours (depending on method).
Regulatory requirements
Ettan Spot Handling Workstation meets or exceeds the standards listed in
Table 6-3.
Table 6-3. Regulatory requirements, Ettan Spot Handling Workstation.
86
Safety
UL 1260, UL 1492 (camera),
CSA C22.2 - No. 151, IEC 1010-1
EMC
EN 50082-1, EN 50022 (frame grabber),
FCC Class A
EMI
EN 50081-1, EN 50024 (frame grabber)
Ettan Spot Handling Workstation System Manual 18-1153-54 Edition AD
Specifications 6
6.4
Ordering information
6.4.1
Accessories
Table 6-4 lists the accessories that can be ordered from GE Healthcare.
Table 6-4. Accessories for Ettan Spot Handling Workstation.
Product
Quantity
Code No.
Ettan Spot Handling Workstation:
Ettan Microplate 96 well, 100 pcs
1
18-1150-42
Reagent bottle
1
18-1158-79
Waste bottle
1
18-1158-81
Sealing for incubator lid, 12 pcs
1
18-1160-40
Gel tray trolley
1
18-1149-79
Picker head 1.4 mm for 1.0 mm thick gels
1
18-1147-03
Picker head 2.0 mm for 1.0 mm thick gels
1
18-1143-64
Reference markers, sheet incl. 560 pcs
2
18-1143-34
Gel holder, 2 pcs
1
18-1156-27
Gel tray incl. gel holders and glass lid
1
18-1150-39
Glass lid for gel tray
1
18-1156-28
Spot Picker:
Syringe, 1 ml
1
18-1143-41
Tubing and fittings
1
18-1143-63
Inlet tubing package
1
18-1156-29
Camera alignment foot
5
18-1143-33
Syringe, 100 μl
1
18-1143-55
Needle
1
18-1166-15
Tubing and fittings
1
18-1166-14
Vial with cap, 1 ml, 100 pcs
1
18-1152-75
Spotter:
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87
6 Specifications
Product
Quantity
Code No.
Ettan Sample Slide Kit
2
18-1147-18
MS sample tray:
Bruker*, Bruker Daltonics
1
18-1155-99
Micromass*, Micromass Ltd.
1
18-1156-00
Voyager™*
1
18-1156-02
Digester:
Tubing and fittings
1
18-1156-31
Syringes, 500 μl
8
18-1170-86
Enzyme container, 48 pcs
1
18-1157-75
Calibration plate
1
18-1156-66
Needles 2 18-1143-57
* Delivered without targets.
88
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Specifications 6
6.4.2
Related products
Table 6-5 list products that are related to Ettan Spot Handling Workstation.
These products can be ordered from GE Healthcare.
Table 6-5. Products related to Ettan Spot Handling Workstation. For code numbers marked with
an asterisk (*), see www.gehealthcare.com.
Product
Code No.
Ettan DALT II large vertical gel system
*
Ettan DALT II Pre-cast Gel 12.5
17-6002-36
Ettan DALT II Buffer Kit
17-6002-50
Immobiline™ DryStrip gels and buffers
*
Trypsin, 10 × 250 ìg for 10 full batch runs
(10 × 1152 samples)
17-6002-75
TFA, 50 ml for 10 full batch runs
(10 × 1152 samples)
17-6002-76
4-HCCA, 10 × 20 mg (pre-weighed) for 10 full
batch runs (10 × 1152 samples)
17-6002-77
ImageMaster 2D Elite Software
80-6350-56
ImageScanner
18-1134-45
ImageScanner Geometry Calibration Kit
18-1145-55
Typhoon 8600
*
Ettan MALDI-ToF Pro 120 V
18-1156-54
Ettan MALDI-ToF Pro 220 V
18-1156-53
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89
6 Specifications
90
Ettan Spot Handling Workstation System Manual 18-1153-54 Edition AD
Index
Index
A
accessories ..........................................................................................................15, 87
associated documentation ................................................................................. 15
attaching
picker head .......................................................................................................... 75
B
backup, results and data ...................................................................................... 49
barcode reader ......................................................................................................... 37
before start-up .......................................................................................................... 46
C
cabinet .......................................................................................................................... 19
calibration plate ........................................................................................................ 63
camera ......................................................................................................................... 27
camera alignment ................................................................................................... 53
changing
digester needles ................................................................................................ 68
picker head .......................................................................................................... 73
spotter needle .................................................................................................... 60
syringes ................................................................................................................. 80
cleaning
regular .................................................................................................................... 52
computer ..................................................................................................................... 19
consumables .............................................................................................................. 45
D
digester ......................................................................................................................... 29
digester needles
changing ............................................................................................................... 68
dryer ............................................................................................................................... 31
E
enzyme cooler ....................................................................................................29, 30
external connections .............................................................................................. 23
F
fan ................................................................................................................................... 19
filter ................................................................................................................................ 19
frame grabber card ................................................................................................ 27
H
hotel ............................................................................................................................... 36
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91
Index
I
I/O controller .............................................................................................................. 19
image scanner .......................................................................................................... 15
ion generator ............................................................................................................. 32
M
maintenance
regular .................................................................................................................... 49
MALDI-ToF MS target ............................................................................................. 17
matrix solution .......................................................................................................... 24
microplate lid ............................................................................................................. 33
O
ordering information .............................................................................................. 87
accessories .......................................................................................................... 87
related products ................................................................................................ 89
P
package contents .................................................................................................... 14
performance specification .................................................................................. 86
picker head ................................................................................................................. 26
attaching ............................................................................................................... 75
changing ............................................................................................................... 73
priming
spot picker/spotter ........................................................................................... 77
R
reagent bottles .......................................................................................................... 17
related products ....................................................................................................... 89
robot .............................................................................................................................. 35
control equipment ............................................................................................ 19
power supply ...................................................................................................... 19
S
schedule, user maintenance .............................................................................. 51
servo gripper .............................................................................................................. 35
specifications ............................................................................................................. 85
spot picker/spotter .................................................................................................. 24
spotter needle
changing ............................................................................................................... 60
starting the workstation ....................................................................................... 45
sub-systems ............................................................................................................... 10
syringe pump ............................................................................................................. 24
system description .................................................................................................. 17
92
Ettan Spot Handling Workstation System Manual 18-1153-54 Edition AD
Index
T
technical specification ........................................................................................... 85
temperature controller .......................................................................................... 19
transfer robot ............................................................................................................. 35
U
user maintenance .................................................................................................... 51
V
video camera ............................................................................................................. 27
W
waste bottles .............................................................................................................. 17
workflow ....................................................................................................................... 38
Ettan Spot Handling Workstation System Manual 18-1153-54 Edition AD
93
Index
94
Ettan Spot Handling Workstation System Manual 18-1153-54 Edition AD
Elanders Östervåla 2006
www.gehealthcare.com
Ettan, ImageMaster, Immobiline, ImageScanner and Typhoon are trademarks
of GE Healthcare companies. GE, imagination at work and GE monogram are
trademarks of General Electric Company.
GE Healthcare Bio-Sciences AB
Björkgatan 30
751 84 Uppsala
Sweden
Microsoft and Windows are either registered trademarks or trademarks of
Microsoft Corporation in the United States and/or other countries.
Decon is a trademark of Decon Laboratories Ltd. Milli-Q is a trademark of
Millipore Corporation. Voyager is a trademark of Applied Biosystems Inc.
All goods and services are sold subject to the terms and conditions of sale
of the company within GE Healthcare which supplies them. GE Healthcare
reserves the right, subject to any regulatory and contractual approval, if
required, to make changes in specifications and features shown herein, or
discontinue the product described at any time without notice or obligation.
Contact your local GE Healthcare representative for the most current
information.
© 2006 General Electric Company – All rights reserved.
GE Healthcare Bio-Sciences AB, a General Electric Company.
GE Healthcare Bio-Sciences AB
Björkgatan 30, SE-751 84 Uppsala, Sweden
GE Healthcare Europe GmbH
Munzinger Strasse 5, D-79111 Freiburg, Germany
GE Healthcare UK Ltd
Amersham Place, Little Chalfont, Buckinghamshire, HP7 9NA, UK
GE Healthcare Bio-Sciences Corp
800 Centennial Avenue, P.O. Box 1327, Piscataway, NJ 08855-1327, USA
GE Healthcare Bio-Sciences KK
Sanken Bldg. 3-25-1, Hyakunincho, Shinjuku-ku, Tokyo 169-0073, Japan
Asia Pacific Tel: +85 65 62751830 Fax: +85 65 62751829 • Australasia Tel: +61 2 8820 8299 Fax: +61 2 8820 8200 • Austria Tel: 01 /57606 1613 Fax: 01 /57606 1614 • Belgium Tel: 0800 73 890
Fax: 02 416 8206 • Canada Tel: 1 800 463 5800 Fax: 1 800 567 1008 • Central, East, & South East Europe Tel: +43 1 972 720 Fax: +43 1 972 722 750 • Denmark Tel: +45 70 25 24 50 Fax: +45 45
16 2424 • Eire Tel: 1 800 709992 Fax +44 1494 542010 • Finland & Baltics Tel: +358 9 512 3940 Fax: +358 9 512 39439 • France Tel: 01 69 35 67 00 Fax: 01 69 41 98 77 • Germany Tel: 0800 9080
711 Fax: 0800 9080 712 • Greater China Tel: +852 2100 6300 Fax: +852 2100 6338 • Italy Tel: 02 26001 320 Fax: 02 26001 399 • Japan Tel: 81 3 5331 9336 Fax: 81 3 5331 9370 • Korea Tel: 82
2 6201 3700 Fax: 82 2 6201 3803 • Latin America Tel: +55 11 3933 7300 Fax: +55 11 3933 7304 • Middle East & Africa Tel: +30 210 96 00 687 Fax: +30 210 96 00 693 • Netherlands Tel: 0800-82
82 82 1 Fax: 0800-82 82 82 4 • Norway Tel: +47 815 65 777 Fax: +47 815 65 666 • Portugal Tel: 21 417 7035 Fax: 21 417 3184 • Russia & other C.I.S. & N.I.S Tel: +7 495 956 5177 Fax: +7 495 956
5176 • Spain Tel: 902 11 72 65 Fax: 935 94 49 65 • Sweden Tel: 018 612 1900 Fax: 018 612 1910 • Switzerland Tel: 0848 8028 10 Fax: 0848 8028 11 • UK Tel: 0800 515 313 Fax: 0800 616 927
USA Tel: +1 800 526 3593 Fax: +1 877 295 8102
imagination at work
18-1153-54 AD 08/2006
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