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Experts In Microplate Analysis
Absorbance
²
Plate Washing
²
Luminescence
²
Automated
Processing
²
Software
DYNEX Technologies, Inc.
14340 Sullyfield Circle, Chantilly, VA 20151-1683
Phone: (703) 631-7800 FAX: (703) 803-1441 www.dynextechnologies.com
User
Manual
Ò
for
MRX
This page is intentionally left blank
MRX
Microplate Reader
User Manual
IMPORTANT
Please read carefully before using the MRX Microplate Reader
Rev. 11-04-02
Software Version:
Instrument Nº:
Part No: 99001840
This document is the copyright of
DYNEX Technologies, Inc. and must not be copied or reproduced in any form without prior consent
.
DYNEX Technologies reserves the right to make technical improvements to this equipment and documentation without prior notice as part of a continuous program of product development. This manual supersedes all previous editions.
MS DOS
®
Licensing
This product contains software licensed from Microsoft Corporation.
1.
The MRX contains intellectual property, that is, software programs, that are licensed for use by the end user customer (hereinafter referred to as the “end user”).
2.
3.
4.
This is not a sale of such intellectual property.
The end user shall not copy, disassemble or reverse compile the software program.
The software programs are provided to the end user “as is” without warranty of any kind, either expressed or implied, including, but not limited to, warranties of merchantability and fitness for a particular purpose. The entire risk of the quality and performance of the software program is with the end user.
5.
DYNEX Technologies and its suppliers shall not be held to any liability for any damages suffered or incurred by the end user (including, but not limited to, general, special, consequential or incidental damages including damages for loss of business profits, business interruption, loss of business information and the like), arising from or in connection with the delivery, use or performance of the software program.
MRX
®
and MRX
plus
®
are registered trademarks of
DYNEX Technologies
.
MRX
II
, Assay Wizard
, and Revelation
are trademarks of
DYNEX Technologies
.
MS DOS
®
, Microsoft
®
, Excel
®
, and Windows
®
are registered trademarks of Microsoft Corporation.
IBM
®
is a registered trademark of International Business Machines Corporation.
Apple
and Macintosh
are trademarks of Apple Computer, Inc.
Copyright
1997,
DYNEX Technologies. .
All rights reserved.
Limitations of Warranty Relating to Software
1.
2.
DYNEX Technologies software. accepts no liability for the maintenance or support of Microsoft
DYNEX Technologies accepts no liability for any consequential loss arising from the use of software on this product outside the scope of the above definition.
Limitations of Use
The user of the MRX must follow the specific manufacturer’s assay package insert when modifying parameters and establishing results calculation methods.
The performance characteristics of the MRX have not been established with specific serology or antigen detection assays. The user must evaluate the MRX in conjunction with the specific serology and/or antigen detection assays. This evaluation must include the establishment of performance characteristics for the specific assays.
Under no circumstances does the MRX have any direct patient contact or perform any therapeutic patient function.
Specific diagnostic results are not provided by the device. Diagnostic decisions are made only after independent confirmation by additional methods under the supervision of a qualified professional.
WARNING:
If this equipment is used in a manner not specified by the manufacturer then the protection provided by the equipment may be impaired.
Before using any decontamination method, except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment.
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Table of Contents
Table of Contents
About This Manual .................................................................. 1
Safety Information.................................................................... 3
Symbols...................................................................................... 5
Chapter 1 Features of the Reader.......................................... 7
Chapter 2 Installation and Setup........................................... 9
Unpacking the Reader .......................................................................... 11
Back Panel ................................................. 12
Back Panel ........................................................... 13
The MRX Front Panel .......................................................................... 15
The Keypad and Display ...................................................................... 15
Windows QWERTY Keyboard Option ................................................ 16
Fitting the Lamp and Optical Filters .................................................... 17
Switching the Reader On...................................................................... 18
Switching between DOS Applications ................................................. 20
Maintenance Menu ............................................................................... 27
Chapter 3 Printer Interface.................................................. 33
Chapter 4 Maintenance......................................................... 35
4.1 Routine Maintenance Procedures....................................................... 37
MRX Microplate Reader User Manual i
ii
External Painted and Plastic Surfaces .................................................. 38
4.3 Repacking the Reader ....................................................................... 39
4.4 Replacing the Lamp .......................................................................... 40
4.5 Changing the Optical Filters ............................................................. 41
Appendix A: Self-Test Diagnostics ....................................... 43
Self-Test Sample Printouts....................................................................... 44
Appendix B: Accessories........................................................ 45
Appendix C: Specifications .................................................. 49
MS DOS Version.................................................................................. 49
Windows Option................................................................................... 49
405 to 850nm Wavelength Range ........................................................ 50
340 to 400 Wavelength Range (Option)............................................... 50
Physical/Environmental Conditions ......................................................... 52
Appendix D: MRX Training Checklist ............................... 53
Run Verification Plate .......................................................................... 53
Review User’s Manual Table of Contents ........................................... 53
Review List of Software Anomalies (If Any) ...................................... 53
Power On -- Self-Diagnostic Test ........................................................ 54
Proper Care of Disks and Backup of Assays and Data Stored on Disks54
Temperature Control ............................................................................ 55
Agglutination Software ........................................................................ 57
MRXplus or MRXII from an External Computer Using Revelation
Pull-Down Menus/Tool Bar/Short Cut Keys/Assay Buttons ............... 59
MRX Microplate Reader User Manual
Table of Contents
Creating an Assay -- Assay Wizard...................................................... 59
Temperature Control Option ................................................................ 61
Agglutination Option............................................................................ 62
Index......................................................................................... 63
MRX Microplate Reader User Manual iii
About This Manual
About This Manual
This manual has been written for the laboratory technician and describes how to use the MRX Microplate Reader.
With the information in this manual, you can:
•
Install the Reader.
•
Configure the Reader, using the Setup menu, to suit your particular needs.
•
Connect the Reader to a printer.
•
Perform basic maintenance procedures.
This manual also describes all the features and specifications of the
Reader hardware and software.
For information on how to use any software you have installed, such as
Endpoint or Barcode software, refer to the individual user manuals.
For information on how to connect the Reader to an external computer, refer to the Endpoint Program User Manual.
For explanations of error messages and possible solutions, refer to the
Troubleshooting Guide.
MRX Microplate Reader User Manual 1
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2 MRX Microplate Reader User Manual
Safety Information
Safety Information
Warning:
Electric
Shock
Hazard
Warning:
Incorrect
Operation
If there is any doubt or concern about the safety of the instrument, contact an approved service center.
Although this instrument is fully insulated and earthed (grounded), it is important for all users to be aware of the potential hazard of using liquids in close proximity to an electrical supply. If any liquids are spilled, disconnect the instrument from the mains electrical supply immediately and clean the liquid up. DO NOT reconnect the electrical supply until the instrument has been fully inspected by an approved service engineer.
Take care when using non-rigid, non-standard microplates as spillages are more likely to occur.
To switch this instrument off, press the power switch on the front panel.
Remove the mains power cable from the socket on the rear panel.
Operating this equipment in ways other than detailed in this manual may impair the protection provided by the instrument.
•
DO NOT operate this equipment with the covers removed as potentially lethal voltages are contained within.
•
DO NOT operate this equipment with the safety earth (ground) disconnected.
•
DO NOT install unauthorized cards, spare components or accessories as this may impair the safety of the Reader and will invalidate its warranty.
•
DO NOT place any objects, or stand within 150mm of the front of the
Reader as this will obstruct the plate carrier.
•
DO NOT overfill the microplate wells as this may lead to liquid contamination of the wells and impair the safety of the Reader.
•
BE PREPARED for unexpected plate carrier movement when the
Reader is controlled by an external computer.
•
MAKE SURE that the voltage ratings on the rear panel of the Reader correspond to the local mains supply.
MRX Microplate Reader User Manual 3
Safety Information
•
MAKE SURE that the mains power cable is correctly wired. Color codes are as follows:
Fuses
Brown Live Black
Green/yellow Earth Ground
The instrument does not contain any user-replaceable internal fuses.
However, the power supply contains an internal fuse in the primary circuit. If the fuse needs to be changed, it must be replaced with a CSA approved 20x5mm, 4A cartridge fuse with a voltage rating of 250V. This fuse should not be replaced by the user. If the fuse blows, contact an approved service center.
4 MRX Microplate Reader User Manual
Symbols
Symbols
Warning Symbol
For your safety the Reader is marked with a warning symbol to remind you of certain hazards:
!
•
On the back panel: this indicates that the optics door may be hot. If you want to remove the optics door, make sure that you allow the
Reader to cool before touching it.
•
Adjacent to the mains outlet: this indicates that the outlet should only be used for an external monitor with a power rating of up to 120VA and an earth leakage current of less than 1 mA at 264V and 60 Hz.
Plate Carrier Diagram
The diagram on the inside of the floppy disk drive door shows the mechanism for moving the plate carrier in and out.
Figure 1 Plate Carrier Diagram
While the Main menu is displayed, the plate carrier can be moved in and out using the up and down (
∧ ∨
) cursor keys. The up key moves the plate carrier in, and the down key moves it out.
Do not place any objects, or stand within 150mm (6.5 in) of the front of the Reader as this will obstruct the plate carrier.
MRX Microplate Reader User Manual 5
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6 MRX Microplate Reader User Manual
Chapter 1 Features of the Reader
Chapter 1 Features of the Reader
The MRX Microplate Reader is a microprocessor-controlled photometer.
It is designed to measure the optical density (OD) of fluid samples in 96well microplates in order to determine the level of chemical reactions.
Figure 2 MRX Microplate Reader
A full list of the Reader’s technical specifications can be found in
Appendix C.
The Reader provides the following features:
•
Endpoint, Kinetic, and Agglutination Software
•
PC-compatibility for additional control and data storage
•
Multi-lingual display which guides you through menu choices
•
Single and dual wavelength reading modes
•
Selection of up to six filter wavelengths
•
Data analysis and presentation as OD matrices and/or graphically using curve fitting routines
•
Data output to printer
MRX Microplate Reader User Manual 7
Chapter 1 Features of the Reader
•
Storage of assay test procedures and microplate data on 3.5" floppy disk drive or hard disk
•
Barcode option
•
Test plate option
•
Temperature Control option
8 MRX Microplate Reader User Manual
Chapter 2 Installation and Setup
Chapter 2 Installation and Setup
Unpacking the Reader .......................................................................... 11
Back Panel ................................................. 12
Back Panel ........................................................... 13
The MRX Front Panel .......................................................................... 15
The Keypad and Display ...................................................................... 15
Windows QWERTY Keyboard Option................................................ 16
Fitting the Lamp and Optical Filters .................................................... 17
Switching the Reader On...................................................................... 18
Switching between DOS Applications ................................................. 20
Maintenance Menu ............................................................................... 27
MRX Microplate Reader User Manual 9
Chapter 2 Installation and Setup
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10 MRX Microplate Reader User Manual
2.1 Installation
2.1 Installation
CAUTION
DO NOT connect the instrument to the mains electrical supply or switch it on before you have completed this installation procedure.
To review the critical functions of installation, operation, maintenance, and software use, refer to the MRX Training Checklist in Appendix D.
Unpacking the Reader
If you ordered an upgrade, you will only receive an upgrade disk and a manual.
The Reader is packed to provide maximum protection during shipment.
Take care when unpacking it and examine it carefully for any damage.
Report any damage to the carrier immediately. Check the contents against the shipping checklist enclosed and report any omissions to your supplier.
Remove the Reader from the carton and place it on a level surface free of dust, moisture, vibration, draughts and away from direct sunlight. (Keep the packing materials so that they can be used if the Reader has to be transported.)
Figure 3 Packing Contents Example
1 Reader 5 Software
3 Optical Filters
6 Power Cord
7 Printer Cable
4 Lamp 8 Interface
9 RS 232 Filter (not shown)
MRX Microplate Reader User Manual 11
2.1 Installation
The MRX
II
and MRX
plus
Back Panel
12
Figure 4 MRX
II
and MRX
plus
Back Panel
1 Mains Inlet 7
2
Voltage Selector (see page 14)
8
3 Monitor Mains Socket
4 Keyboard Socket
9
10
Optics Door
Optics Cooling Vents
Serial Number Plate
PSU Cooling Vents
5 Serial Port 11 Main Enclosure
!
Notes:
1. The warning symbols on the back panel indicate that the optics door may be hot, and that no appliances other than a monitor should be plugged into the mains outlet. For more details, refer to the discussion on
Symbols earlier in this manual.
2. The positions of ports 5 and 6 may not be exactly as shown, but the number of pins or pin sockets in each port will remain the same. styrofoam packing inside the Optics Door (#7).
MRX Microplate Reader User Manual
The MRX
Revelation
Back Panel
2.1 Installation
Figure 5 The MRX
Revelation
Back Panel
1 Mains Inlet 8 Monitor Port
2
Voltage Selector (see page 14)
9 Optics
3 Monitor Mains Socket
4 Keyboard Socket
10 Optics Cooling Vents
11 Serial Number Plate
12 PSU Cooling Vents 5 PS/2 Mouse Socket
6 Serial Port 13 Main Enclosure
!
Notes:
1. The warning symbols on the back panel indicate that the optics door may be hot, and that no appliances other than a monitor should be plugged into the mains outlet. For more details, refer to the discussion on
Symbols earlier in this manual.
2. The positions of ports 5 through 8 may not be exactly as shown, but the number of pins or pin sockets in each port will remain the same. styrofoam packing inside the Optics Door (#7).
MRX Microplate Reader User Manual 13
2.1 Installation
Voltage Selector
CAUTION
The position of the voltage selector (see Figure 4 or Figure 5) is very
important and must be adjusted to match the line voltage of the mains power supply before switching the Reader on.
Position of Voltage Selector Mains Line Voltage
115V 100-120V
230V 200-240V
FAILURE TO FOLLOW THE PROCEDURES DETAILED
BELOW MAY RESULT IN DAMAGE TO THE MICROPLATE
READER DUE TO PARTS FALLING INTO THE POWER
SUPPLY.
There are two types of plastic cover fitted to the voltage selector switch:
•
If the plastic cover has a slot cut in it and the voltage selector is not in the correct position, insert a suitable small flat-bladed screwdriver through the slot and move the selector to the desired voltage. DO
NOT LOOSEN THE SCREWS USED TO RETAIN THE PLASTIC
COVER.
•
If the plastic cover does not have a slot cut in it and the voltage selector is not in the correct position, use a No. 1 Posi driver to remove one of the screws and loosen the other screw to allow the plastic cover to be pushed aside. Move the switch to the desired voltage, replace the cover and tighten the screws. DO NOT REMOVE
BOTH SCREWS USED TO RETAIN THE PLASTIC COVER.
14 MRX Microplate Reader User Manual
The MRX Front Panel
2.1 Installation
Figure 6 The MRX Front Panel
1
Power Switch (marked I/O for on/off)
2 Power Light
3 Plate Carrier Door
4 Floppy Disk Drive
5 Keypad and display
The Keypad and Display
The Reader is controlled by menu-driven software which responds to the user's menu choices. The menus are displayed on a liquid crystal display which can show up to five options at a time. Choices are entered using the keypad.
Figure 7 Function Keys and Display
•
Function keys – menu options are shown on the bottom line of the display and selected by pressing the function key below.
MRX Microplate Reader User Manual 15
2.1 Installation
16
Figure 8 Alphanumeric Keys and Cursor Keys
•
Alphanumeric keys – used to enter numbers and well locations.
•
Esc key – returns the display to the Main menu.
•
Cursor keys – used to scroll through well locations and menus, and
(in some menus) to move the plate carrier in and out.
!
computer, the keypad will not operate.
Windows QWERTY Keyboard Option
If you have bought the MRX
Revelation,
you will have to install the monitor, keyboard and mouse.
Keyboard 1. Plug the keyboard cable into the Keyboard Socket
2. Place the protective cover over the keys.
Monitor
1. Place the monitor on top of the Reader.
2. Plug the power cord into the Monitor Mains Socket
3. Plug the monitor cable into the Monitor Port (labeled
Mouse 1. Plug the mouse cable into the PS/2 Mouse Socket
If you are also running DOS programs, they will not run correctly while the mouse is plugged in. Always
unplug the mouse before running a DOS program.
MRX
Revelation
. Do not install any unauthorized hardware or software, as this may affect the reliability of the Reader and will invalidate the warranty.
MRX Microplate Reader User Manual
2.1 Installation
Fitting the Lamp and Optical Filters
Warning
Disconnect the mains cable before fitting lamp and filters.
!
1. Using a No. 1 Posi driver remove the optics door and, if fitted, remove the packing materials from behind the door.
1. Slide the optics assembly out of the instrument.
If you have an older instrument (check your serial number panel: newer Readers have 1CXCxxxx or 2CXCxxxx numbers), you need to disconnect the optics assembly’s 10-way connector and then slide the
assembly out of the instrument.
1. Insert the lamp into the assembly and plug the connector into its back
Make sure that the lamp is seated correctly with the locating pip on the lamp fitted into the notch on the optics assembly.
Figure 9 Fitting the Lamp and Filters
4. Insert the filters into the filter wheel. a) Place the filter with the lowest wavelength in position 1, the next lowest in position 2 and so on. a) Make sure the side of the filter that has the shorter distance from the groove to the end is toward the lamp. a) The Filter Blanks must be fitted into the unoccupied filters positions in the Filter Wheel. a) Make sure the Filter Springs are around the sides of the Filter as
fail due to “Filter Motor Errors.”
MRX Microplate Reader User Manual 17
2.1 Installation
Figure 10 Correct Filter Spring Positioning (view of Filter Wheel from filter spring side)
18
Figure 11 Incorrect Filter Spring Positioning
5. Slide the optics assembly back in (if you have an older instrument,
you must also reconnect the 10-way connector) and replace the optics door. Attach the lamp connector to back of lamp.
Switching the Reader On
Before switching the Reader on, note the position of the voltage selector
(see Figure 4 or Figure 5). The position of the selector is very important
and must be adjusted to match the line voltage of the mains power supply.
Position of Voltage Selector Mains Line Voltage
115V 100-120V
230V 200-240V
If the voltage selector is not in the correct position, refer to the Voltage
Selector discussion on page 14.
DO NOT place any objects, or stand within 150mm of the front of the
Reader as this will obstruct the plate carrier.
MRX Microplate Reader User Manual
2.1 Installation
1. Make sure that the instrument is switched off. The power switch is
ON when it is flush with the front panel and OFF when it stands out from the panel.
2. For a MRX
II
: insert a Reader program disk into the floppy disk drive.
3. Insert the power cord supplied into the socket at the rear of the instrument.
4. Connect the power cord to a suitable power source with a protective earth (ground).
5. Connect the printer cable from the Reader to the printer.
6. Switch the printer on.
7. Switch the Reader on by pressing the power switch on the front panel.
8. Verify that the Reader completes the self-test routine successfully.
Self-Tests
When the Reader is switched on, it carries out several self-tests to check that the instrument is working correctly. Appendix A shows a list of these tests and a sample printout. During the self-tests, a series of symbols are displayed on the bottom line of the LCD: these indicate the optional extras that are installed in your Reader.
If the Reader fails any of the tests, an error message is displayed. If the
Reader's data files have been corrupted, the Reader requests your serial number (this can be found on the Reader’s back panel or inside of the floppy disk drive’s door). If an error message persists, contact the service center.
Maintenance Reminder
On first power up, the Reader may display a maintenance reminder.
HAVE YOU PERFORMED YOUR
MAINTENANCE CHECKS ?
To clear the message, press any key. The reminder is intended for use by laboratories which routinely verify the operation of their instruments.
The self-test printouts and the frequency of the maintenance reminders may be altered from the SETUP menu.
Main Menu
If no faults are detected, the instrument displays the Main menu which shows all the available software programs and SETUP. If there are more
MRX Microplate Reader User Manual 19
2.1 Installation than four software programs installed, use the < and > cursor keys to view them.
While the Main menu is displayed the plate carrier can be moved in using the
∧
cursor key and out using the
∨
cursor key. The plate carrier should not be left out of the Reader for more than a few minutes at a time as it will gather dust and may become damaged. If it is left for five minutes without using the software, the Reader will move the plate carrier in automatically.
Switching between DOS Applications
On a MRX
II
:
1. Press the Esc key to return the Reader to the Main menu.
1. Switch the Reader off.
1. Change the software disk.
1. Switch the Reader on again.
!
to re-enter them for the new program.
On a MRX
plus
:
1. Press the Esc key to return the Reader to the Main menu.
1. Select the required program using one of the function keys.
20 MRX Microplate Reader User Manual
2.2 Setup Procedures
2.2 Setup Procedures
The Reader has a number of settings which can be adjusted to suit the assays which will be run on it. The settings are altered using the Setup menu. To use the Setup menu options, select SETUP from the Main menu. The options accessed by this menu are summarized in the table below and described in detail in this section.
Menu Setting
FILTERS
Number of
Filters
Wavelengths
CLOCK
Time mode
Date mode
Value
1/2/3/4/5/6
340 – 850 nm
12/24 hour dd.mm.yy/mm.dd.yy
Language English/French/Italian
German/Spanish
Display 2x40
SYSTEM
MAINT.
COMMS
Printer various
Print self test yes/no
Retest yes/no
Reminder no/daily/weekly/monthly
Comms modes
Comms Ports
MRX/MR700/Custom
COM1
PARAM
Over/Under limit
Data Conversion
Plate Type
0 – 4.000 (3.500) yes/no
12x8/10x4
Repeatability 1 – 4 readings
!
Note: If you are using a MRX
II
Reader and you make changes to the
Setup settings, these changes will only apply to the current software program. If you insert a different program disk, you will have to re-enter the settings.
MRX Microplate Reader User Manual 21
2.2 Setup Procedures
Setup Menu
The Setup menu allows you to view and if necessary alter, the Reader settings.
SETUP ? 4:45P 30.06.94
OTHER FILTERS CLOCK SYSTEM CE
Select OTHER to access a second Setup menu
SETUP? 4:45P 30.06.94
MAINT. COMMS MEMORY PARAM CE
Example
In the menus shown on the following pages, the options available are shown on the bottom line of the display. To select an option press the function key below it or, where appropriate, key in a value.
!
shown. To enter a different value you must press CE. This will access the alternatives available.
To change the display language from English to Spanish:
Select SYSTEM from the Setup menu to access the System menu. Select
LANGUAGE from the System menu to access the Language menu.
The default or previously defined menu language is displayed:
LANGUAGE : ENGLISH 4:45P 30.06.94
ENTER CE
To access the alternative languages select CE.
LANGUAGE ? 4:45P 30.06.94
ENGLISH FRANCAIS DEUTSCH ITALIANO OTHER
Select one of the languages displayed by pressing the function key directly below it, or select OTHER to access the Spanish option.
LANGUAGE ? 4:45P 30.06.94
ESPANOL CE
To select Spanish as the display language press the function key below
ESPANOL. The menus will then be displayed in Spanish.
22 MRX Microplate Reader User Manual
2.2 Setup Procedures
Filters Menu
The Filters menu allows you to install new filters and enter filter wavelengths.
Select FILTERS from the Setup menu to display the number of filters installed:
NO. OF FILTERS = 2 4:45P 30.06.94
ENTER CE
ENTER
Confirm the number of filters shown.
CE
Clear the number of filters and enter a new number.
Minimum: 1 filter. Maximum: 6 filters.
Once the number of filters has been entered, the Reader prompts you to enter the wavelength of each filter in turn.
!
wavelength should be in position 1 on the filter wheel, and so on in ascending order of wavelength.
The Reader will display Filter 1 and a wavelength, for example:
FILTER 1=410 nm 4:45P 30.06.94
ENTER CE
Select ENTER to confirm the wavelength shown and move on to Filter 2.
Select CE to clear the wavelength shown, and enter a new wavelength.
Press ENTER.
When wavelengths have been entered for all the filters installed, the display will return to the Setup menu.
MRX Microplate Reader User Manual 23
2.2 Setup Procedures
24
Clock Menu
Select CLOCK from the Setup menu to access the Clock menu and view the time and date settings.
SETUP? 4:45P 30.06.94
TIME DATE CE
Time
Select TIME to set the clock mode and time. The Reader will display the current setting. Select ENTER to confirm the mode shown, or select CE to display the alternatives.
CLOCK MODE ? 4:45P 30.06.94
12-HOUR 24-HOUR CE
Select 12-HOUR or 24-HOUR mode.
The Reader will allow you to change the time display.
TIME = 4:45P 30.06.94
ENTER CE
Select ENTER to confirm the time shown or enter the correct time using the keypad.
•
If you selected the 24-hour mode, enter the time and press ENTER.
•
If you selected the 12-hour mode, enter the time and then select A.M. or P.M.
Date
Select DATE to display the date mode. The Reader will display the current setting. Select ENTER twice to confirm the mode shown, or select CE to display the alternatives.
DATE MODE ? 4:45P 30.06.94 dd.mm.yy mm.dd.yy CE
Select dd.mm.yy or mm.dd.yy.
The Reader will allow you to change the date display.
DATE = 4:45P 30.06.94
ENTER CE
Select ENTER to confirm the date shown or type in a new date. To edit the date entered, select CE, use the keypad to enter a new date, and then select ENTER.
MRX Microplate Reader User Manual
2.2 Setup Procedures
System Menu
Select SYSTEM from the Setup menu to access the System menu and view the printer and user interface settings.
SETUP ? 4:45P 30.06.94
LANGUAGE DISPLAY KEYPAD PRINTER CE
Language
Select LANGUAGE to set the language options. The Reader will display the current language setting. Select ENTER to confirm this setting or CE to access the options:
LANGUAGE ? 4:45P 30.06.94
ENGLISH FRANCAIS DEUTSCH ITALIANO OTHER
OTHER accesses a second Language menu with the ESPANOL option.
Select the required language, or select CE to return to the System menu.
The language chosen will be used to display all subsequent menus.
Display
Select DISPLAY to view the Reader display format.
DISPLAY : 2 X 40 4:45P 30.06.94
ENTER CE
Press CE or ENTER to return to the System menu.
Keypad
Select KEYPAD to view the Reader keypad format.
KEYPAD : 21 KEY 4:45P 30.06.94
ENTER CE
Select ENTER to move on. The keypad has an optional beep which sounds each time a key is pressed. The Reader will display the current key beep setting. Select ENTER to confirm the setting, or select CE to access the options:
KEY BEEP? 4:45P 30.06.94
YES NO CE
Printer
Select YES to switch the beep on, or NO to switch it off.
Select PRINTER to view the printer settings.
MRX Microplate Reader User Manual 25
2.2 Setup Procedures
Page Size
Lab Name
EPSON LX-810 4:45P 30.06.94
NEXT LAST ENTER CE
Select ENTER to accept the printer shown or use the NEXT and LAST commands to scroll through the list of printers.
The Reader is supplied with a number of standard printer drivers. If your printer is not listed here, contact the service center.
The Reader asks you to enter the page size (in inches). For example:
PAGE LENGTH = 11.000 4:45P 30.06.94
ENTER CE
Select ENTER to accept the page length and width shown or press CE to enter new values.
The Reader asks if you want to print a laboratory name at the top of each print-out sheet.
LAB. = 4:45P 30.06.94
YZ ABCD ENTER CE
Enter a laboratory name, or select ENTER to return to the System menu.
Laboratory names may be up to 16 characters in length and consist of the following characters.
•
Numbers 0–9 and a decimal point. Enter these using the keypad.
•
Upper-case letters, lower-case letters and symbols. Enter these in the following way:
a)
Choose upper-, or lower-case letters or the symbols using the
∧∧∧∧
and
∨∨∨∨ cursor keys.
a)
Scroll through the display characters on the display using the < and > cursor keys.
a)
Enter the character shown by the flashing cursor by pressing the
∧∧∧∧ function key below it.
When the name is complete, select ENTER.
26 MRX Microplate Reader User Manual
2.2 Setup Procedures
Maintenance Menu
Select MAINT. from the Setup menu to access the Maintenance menu and view the self-test printouts and maintenance reminder settings.
Self-Tests
Self-tests are performed when the Reader is switched on, to make sure everything is working correctly. The self-test can be printed, if necessary, so that a record can be kept. The Reader will display the current self-test print setting. Select ENTER to confirm the setting or CE to access the options.
PRINT SELF-TEST ? 4:45P 30.06.94
YES NO CE
Select YES to print the self-tests each time they are run.
!
test to print.
An extra self-test (retest) is also available which is carried out before each plate is read. The Reader will display the current setting for the test.
Select ENTER to confirm the current setting or CE to access the options.
TEST BEFORE READ ? 4:45P 30.06.94
YES NO CE
Select YES to perform the extra test or NO to omit it.
Maintenance Reminder
The Reader has a maintenance reminder which can be displayed at regular intervals on power up. The reminder is intended for use by laboratories which routinely verify the operation of their instruments.
The Reader will display the current setting. Select ENTER to confirm the current setting or CE to access the options.
MAINTENANCE ? 4:45P 30.06.94
NO DAILY WEEKLY MONTHLY CE
Select NO if you do not want any reminders to be displayed, or select the appropriate frequency.
MRX Microplate Reader User Manual 27
2.2 Setup Procedures
Comms Menu
Select COMMS from the Setup menu to access the Communications menu and view PC communications settings.
!
communications protocols.
SETUP ? 4:45P 30.06.94
MR700 MRX USER CE
MR700
Communications
Port
Select MR700 to set the Reader communication parameters to a format suitable for all DYNEX Technologies application programs. The MR700 settings are:
Baud Rate
Stop Bits
9600
1
Data Bits 8
Parity even
Data Format MR700
The Reader asks which communication port is being used. Select COM1 or COM2 to specify the port, or select CE to return to the Comms menu.
MRX
Select MRX to set the Reader communication parameters to an enhanced format suitable for most application programs. When MRX is selected, the display flashes briefly and then redisplays the setup menu.
The MRX settings are:
Baud Rate
Stop Bits
Data Bits 8
Parity even
Data Format
9600
1
MRX
Communications
Ports
The Reader asks which communication port is being used. Select COM1 or COM2 to specify the port, or select CE to return to the Comms menu.
!
II
, no COM2 option exists.
28 MRX Microplate Reader User Manual
2.2 Setup Procedures
User
Select USER to customize the communication settings. The User options allow you to set the values for baud rate, stop bits, data bits, parity and data format.
The settings available are:
Baud Rate
Stop Bits
110/150/300/600/1200/2400/4800/9600
1/2
Data Bits 7/8
Parity none/even/odd
Data Format MR700/extended/MRX
Memory Menu
Select MEMORY from the Setup menu to access the Memory menu which allows you to reset the Reader memory or to upgrade the software.
SETUP MEMORY ? 4:45P 30.06.94
RESET UPGRADE CE
To upgrade the Reader, follow the instructions provided with the upgrade disk.
Reset
The RESET command resets the Reader’s default settings, overwriting all user-defined setup parameters. The Reader will ask you to confirm this action.
ARE YOU SURE ? 4:45P 30.06.94
YES NO CE
Select YES to reset all the Reader’s parameters, or NO to return to the
Memory menu.
Upgrade
The UPGRADE option allows you to upgrade the Reader with a software upgrade disk. Only Reader application software obtained from your supplier should be used with this command.
If you are upgrading the Main menu Setup software, refer to “Upgrading
From a Previous Version” in Chapter 3 in the Endpoint Program User
Manual.
MRX Microplate Reader User Manual 29
2.2 Setup Procedures
Param Menu
Select PARAM from the Setup menu to access the Parameters menu and view the OD limit, data conversion, plate type and repeatability settings.
Over Limit
The Over limit is used to set a range within which ODs should fall. ODs above the limit will not be processed. The limit is also used as an Under limit to set the negative limit of the range.
For example: If 3.500 is set as the over limit, ODs above 3.5 will not be stored and will be printed as “OVER”. ODs below -3.5 will be printed as
“UNDER”.
The Reader will display the current Over Limit. Select ENTER to confirm the value shown and move on to the Param Plate Type menu.
Select CE to clear the value shown, and enter a new value.
Minimum: 0.000. Maximum: 4.000.
!
results may be rejected. The normal limit is 3.500.
Data
Conversion
If the Over limit is altered, the Reader asks if ODs which lie outside the range should be assigned a conversion value for use in quality control equations. If no value is assigned to these ODs, the QC equations which contain them will fail.
DATA CONVERSION 4:45P 30.06.94
YES NO ENTER CE
Select YES to enter a value for Over and Under ODs or select NO to define the plate type. The Reader will ask for the Over value, followed by the Under value. For example:
OVER VALUE =9.999 4:45P 30.06.94
ENTER CE
Enter Over and Under values and select ENTER.
Min. Over value: 0.000. Max. Over value: 9.999.
Min. Under value: -9.999. Max. Under value: 0.000.
Plate Type
Two types of plate format can be used with the Reader. One is the standard 12 rows by 8 columns format and the other is the 10 rows by 4 columns format or the “Chinese plate” format.
The Reader will display the current plate type setting.
30 MRX Microplate Reader User Manual
2.2 Setup Procedures
Select ENTER to confirm the current plate type setting, or CE to access the options.
PLATE TYPE ? 4:45P 30.06.94
12x8 10x4 CE
Select 12x8 or 10x4 to the specify the plate type.
Repeatability Mode
Using the repeatability mode you can specify that each reading the
Reader takes is repeated a number of times and averaged into a single result.
The Reader will display the current repeatability setting. Select ENTER to accept the current setting and return to the Setup menu, or select CE to enter a different number.
REPEATABILITY MODE = 4:45P 30.06.94
ENTER CE
Minimum: 1 (no reading repeats). Maximum: 4.
option should be used with caution for time-critical plate readings.
MRX Microplate Reader User Manual 31
2.2 Setup Procedures
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32 MRX Microplate Reader User Manual
Chapter 3 Printer Interface
Chapter 3 Printer Interface
If the Reader has been supplied with a printer, this printer will be completely compatible with it. The following information is for users whose printer was not supplied with the Reader.
Any printer which communicates through a Centronics parallel printer interface will operate successfully with the Reader.
!
switched on before switching on the Reader.
An external printer can be connected to the Reader using the interface cable shipped with the instrument. This is a ribbon cable terminated by a
standard IEEE / Centronics style, 36 way connector (see Figure 12).
Figure 12 Printer Cable Connector (Centronics)
The table below shows the relevant pin assignments.
Pin No Signal Name Pin No Signal Name
MRX Microplate Reader User Manual 33
Chapter 3 Printer Interface
11 BUSY 29
12 PE
13 SLCT 31
14
15
16
17
18
Not Used
Not Used
Not Used
Not Used
Not Used
32
33
34
35
36
Not Used
Not Used
Not Used
Not Used
Not Used
34 MRX Microplate Reader User Manual
Chapter 4 Maintenance
Chapter 4 Maintenance
4.1 Routine Maintenance Procedures....................................................... 37
External Painted and Plastic Surfaces .................................................. 38
4.3 Repacking the Reader ....................................................................... 39
4.4 Replacing the Lamp .......................................................................... 40
4.5 Changing the Optical Filters ............................................................. 41
MRX Microplate Reader User Manual 35
Chapter 4 Maintenance
This page is intentionally left blank.
36 MRX Microplate Reader User Manual
4.1 Routine Maintenance Procedures
IMPORTANT
The first section in this manual contains safety information for all the equipment in the system. You should be familiar with this information anyway, but if not, you must read through this section before carrying out any maintenance.
The warranty on the Reader may become void if you do not follow the safety and maintenance instructions contained in this manual, or if the covers are removed by anyone other than approved service personnel.
4.1 Routine Maintenance Procedures
The Reader requires very little maintenance, however, certain simple procedures should be carried out on a regular basis.
On a daily basis:
•
Verify self test passes.
•
Remove the microplate from the plate carrier, wipe the plate carrier down and return it to the In position.
•
Wipe down the external surfaces. Section 4.2 contains more detailed cleaning instructions.
On a weekly basis:
•
Review the results of the self-test that occurs when you switch the
Reader on to make sure the instrument is functioning correctly. If you want to retain this information for your records, print out the self-test results (see Appendix A for a sample) or save them to a file.
•
Back up the data files for a MRX
II
(remove the disk and back it up using the File Manager program on an external PC).
On a yearly basis:
•
Remove the filters and clean them. Section 4.2 contains more detailed cleaning instructions.
Periodic maintenance should be determined by the user.
MRX Microplate Reader User Manual 37
4.2 Cleaning
4.2 Cleaning
Warning
Always switch the instruments off and disconnect the power cable before cleaning the Reader.
The Reader is constructed from high quality materials, nevertheless spilt saline solutions, solvents, acids or alkaline solutions must be removed from outer surfaces immediately to prevent damage.
Autoclaving
DO NOT autoclave any part of this instrument.
External Painted and Plastic Surfaces
Clean external surfaces with a mild laboratory detergent, followed by
10% bleach or 70% alcohol. Always dilute detergents according to the manufacturer's instructions.
Filters
Clean the filters by wiping them with a lint-free cloth or a lens tissue. If necessary a little alcohol may be used with the cloth or tissue.
Decontamination
If you need to return the Reader to the service center, and it has been in contact with human blood, other potentially infectious body fluids, pathological samples, toxic or radioactive materials, it must be decontaminated.
Before shipping the instrument, do the following:
•
Clean the surface with a mild laboratory detergent followed by 10% bleach.
•
Fill out an Equipment in Transit form (refer to Appendix B for a copy of the form).
38 MRX Microplate Reader User Manual
4.3 Repacking the Reader
4.3 Repacking the Reader
This procedure assumes that the original Reader packaging materials are
being used. Figure 13 summarizes the repacking instructions.
1. Remove the optics door and take out the lamp and filters. When you replace the optics door, insert the optics door packing materials.
1. Pack the lamp and each of the filters separately.
1. Make that there is no microplate in the plate carrier, and no disk in the floppy drive.
1. Place the Reader in the original anti-static plastic bag.
1. Place the bottom end caps into the carton and lower the Reader into the carton. Fit the top end caps to the Reader.
1. Place the filters and lamp in the accessories box and fit this between the end caps.
1. Seal the box carefully and securely. Make sure that the box is not dropped or handled in a rough manner.
Figure 13 Repacking the Reader
MRX Microplate Reader User Manual 39
4.4 Replacing the Lamp
4.4 Replacing the Lamp
Warning
If the Reader has been used recently, the optics door and the lamp in the optics assembly may still be hot. Allow time for the Reader to cool before touching it. Take care when removing the optics door as there may be broken glass in the optics assembly.
Figure 14 summarizes the lamp replacement instructions.
1. Switch off the mains power supply and disconnect the cable.
1. Using a No. 1 Posi driver remove the optics door.
1. Slide the optics assembly out of the instrument.
If you have an older instrument (check your serial number panel: newer Readers have 1CXCxxxx or 2CXCxxxx numbers), you need to disconnect the optics assembly’s 10-way connector and then slide the
assembly out of the instrument.
1. Detach the lamp from the assembly and replace with a new one. Make
sure that the lamp is seated correctly (refer to “Fitting the Lamp and
1. Slide the optics assembly back in (if you have an older instrument, you
must also reconnect the 10-way connector).
1. Replace the optics door and reconnect the power supply.
Figure 14 Replacing the Lamp
40 MRX Microplate Reader User Manual
4.5 Changing the Optical Filters
4.5 Changing the Optical Filters
Warning
If the Reader has been used recently, the optics door and the lamp in the optics assembly may still be hot. Allow time for the Reader to cool before touching it. Take care when removing the optics door as there may be broken glass in the optics assembly.
Figure 15 summarizes these instructions.
1. Switch off the power supply and disconnect the mains cable.
1. Using a No. 1 Posi driver remove the optics door.
1. Slide the optics assembly out of the instrument.
If you have an older instrument (check your serial number panel: newer Readers have 1CXCxxxx or 2CXCxxxx numbers), you need to disconnect the optics assembly’s 10-way connector and then slide the
assembly out of the instrument.
1. Remove the relevant filter from its socket in the filter wheel, and replace with the new one.
•
Make sure the side of the filter that has the shorter distance from the groove to the end is toward the lamp.
•
Make sure the Filter Springs are around the sides of the Filter as
Otherwise, the self-test will fail due to “Filter Motor Errors.”
1. Slide the optics assembly back in (if you have an older instrument, you
must also reconnect the 10-way connector).
1. Replace the optics door and reconnect the power supply.
Figure 15 Changing the Optical Filters
!
certain to run PARAMS to establish new baselines.
MRX Microplate Reader User Manual 41
4.5 Changing the Optical Filters
This page is intentionally left blank.
42 MRX Microplate Reader User Manual
Appendix A: Self-Test Diagnostics
Appendix A: Self-Test Diagnostics
On power up and (optionally) before every read, the Reader performs several self-tests. These cover:
A/D Status
•
Begins conversion and checks that the status line goes high and low.
•
Verifies that hardware can be detected and chosen.
Plate Motor
•
Moves the plate to Out position and then to the
In position.
•
Verifies that the plate carrier position sensors are operating correctly.
Background
Light
•
Checks motor and sensors.
•
Checks for light leakage into the reading compartment.
Bulb
•
Verifies that current is flowing through the bulb when it is switched on.
•
Checks that the bulb has not blown.
Filter Motor
•
Moves the filter motor to the first position.
•
Checks the filter motor and optical alignment.
Transmission
Levels
•
Verifies that all fibres are capable of transmitting at least 33% of the maximum transmission level.
•
Checks for blocked channels or broken diodes.
Filters
•
Checks that gains can be selected for all filters.
•
Checks for damaged or missing filters.
MRX Microplate Reader User Manual 43
Appendix A: Self-Test Diagnostics
Self-Test Sample Printouts
SELF-DIAGNOSTIC SEQUENCE
Reader Serial # 2CXC2549 Technologist: . . . ..
Time : 11:15:02 AM
Test 1 : A/D
Test 2 : Plate motor
Test 3 : Background light levels
Test 4 : Bulb
Test 5 : Filter motor
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Test 6 : Transmission levels
Test 7 : Filters
Self-Diagnostic Summary
Reader Status : Ready
44 MRX Microplate Reader User Manual
Appendix B: Accessories
Appendix B: Accessories
Barcode Reader, Wedge
Cable, Printer
Cable, RS-232 (9 pin to 9 pin)
Cable, RS-232 (9 pin to 25 pin)
Euro Power Cord
Filter 340nm
Filter 405nm
Filter 450nm
Filter 490nm
Filter 540nm
Filter 550nm
Part Number
0119780410
1119760008
1119760007
50600120
6090405008
6329780340
6329780405
6329780450
6329780490
6329780540
6329780550
Filter 620nm
Filter 630nm
6329780620
6329780630
Lamp (UV)--shipped standard with single board unit
549000600
Dust Cover (MRX
II
and MRX
plus
)
9119780012
Dust Cover (MRX
Revelation
)
9119780011
Lens Cleaning Cloth 41000070
Service Manual single board 99000500
RS-232 Filter, 9 way D Connector 50400380
Test Plate 12 way (non UV) 450nm with Manual, Insert, and Sofware
0119780004
Test Plate 12-way (UV) with
Manual, Insert, and Sofware
Test Plate Software (for use with single board unit only)
US Power Cord
0119780001
04000100 (upgrade for MRX
plus
04000110 (system for MRX
II
)
6090405009
)
MRX Microplate Reader User Manual 45
Appendix B: Accessories
MRX
II
User Manual
MRX
plus
User Manual
MRX
Revelation
User Manual
17000010
17000020
17000030
46 MRX Microplate Reader User Manual
Appendix B: Accessories
MRX Microplate Reader User Manual 47
Appendix B: Accessories
This page is intentionally left blank.
48 MRX Microplate Reader User Manual
Appendix C: Specifications
Appendix C: Specifications
Software Specifications
Menu language
Clock function
MRX
II
MRX
plus
MRX
Revelation
Program Load
Time
English/French/German/Italian/Spanish
Day, date and time
Standard:
Endpoint Program
Optional:
Kinetic Program and Agglutination
Program
Standard:
Endpoint Program, Kinetic Program, and Agglutination Program
Standard:
Revelation Program
Using MRX
Using MRX
II
:
plus
:
< 40 seconds
< 1 second
Hardware Specifications
MS DOS Version
Keypad
21 key tactile membrane
Display
2 line by 40 character LCD
No. of filters
2-6
Windows Option
Keypad
Display
QWERTY
Super VGA Color
No. of filters
2-6
Performance
Read cycle time
Single Wavelength: < 4 seconds
Dual Wavelength: < 6 seconds
MRX Microplate Reader User Manual 49
Appendix C: Specifications
405 to 850nm Wavelength Range
Dynamic range
-0.100 to +4.000 OD
Linearity
†
Accuracy
0.000 to 2.000 OD:
±
1.0%
2.001 to 3.500 OD:
±
1.5%
Filter band width
(FWHM)
10 nm ± 2 nm
Center Wavelength Accuracy
Precision*
±
2 nm
0.000 to 2.000 OD:
0.2% CV or 0.005 OD whichever is greater
2.001 to 3.000 OD:
0.6% CV
3.001 to 3.500 OD:
1.0% CV
0.000 to 3.500 OD:
2.5% or 0.005 OD whichever is greater
340 to 400 Wavelength Range (Option)
Dynamic range
-0.100 to +3.000 OD
Linearity
†
0.000 to 2.500 OD:
±
2.0%
Filter band width
(FWHM)
18nm ± 2 nm
Center Wavelength Accuracy
Precision*
±
2 nm
0.000 to 2.000 OD:
0.6% CV or 0.005 OD whichever is greater
2.001 to 2.500 OD:
1.0% CV
† Linearity is the maximum percentage deviation of a reading from the ideal straight line plot of OD versus concentration. (Measured by the manufacturer at 410nm in the visible range and 340nm in the UV range).
* Precision is the mean Coefficient of Variation (CV) figure for all wells on a plate that has been read 20 times in succession. (Measured by the manufacturer at 410nm in the visible range and 340nm in the UV range).
50 MRX Microplate Reader User Manual
Appendix C: Specifications
Interface
Printer Interface
Parallel Centronics Compatible with up to 850 printers.
All printers must be compliant with EMC directive 89/336.
A number of standard printer drivers are included. If your printer is not one of these, contact the service center.
Computer Interface
Bi-directional RS232C with user-definable protocol.
Electrical
Light source
Mains input
Power consumption
Fuses
75W tungsten halogen lamp
100 to 120V or 200 to 240V
50 to 60 Hz
500 VA
There are no internal user-replaceable fuses. The power supply contains a CSA approved 20x5mm, 4A cartridge fuse with a voltage rating of 250V in the primary circuit. If the fuse blows, contact the service center.
5A
Mains Power Fuse
(U.K. mains plug/powercord)
Safety
Class I apparatus
MRX Microplate Reader User Manual 51
Appendix C: Specifications
Physical/Environmental Conditions
Weight
Dimensions
12.5 kg (27.5lb)
D390 x W360 x H220 mm
Operating Temperature
10 – 40
°
C
Operating Humidity
10 – 90% RH
Storage Temperature
0 – 50
°
C
Storage Humidity
10 – 90% RH
Altitude
< 2000m
52 MRX Microplate Reader User Manual
Appendix D: MRX Training Checklist
Appendix D: MRX Training Checklist
The MRX Training Checklist is provided as a guide to ensure the critical functions of installation, operation, maintenance and use of software have been reviewed.
MRX All Types
# Reader set-up
# Serial number
# Power outlet, on/off button, communication ports and floppy drive
# Lamp, filters and software
# Printer set-up (and other peripherals)
Maintenance
# Replace lamp
# Change filters
# Cleaning
Run Verification Plate
Review User’s Manual Table of Contents
Review List of Software Anomalies (If Any)
MRX Microplate Reader User Manual 53
Appendix D: MRX Training Checklist
MRX
II
Power On -- Self-Diagnostic Test
# Print self-test
Proper Care of Disks and Backup of Assays and Data
Stored on Disks
Endpoint Software
Set-Up
# Filters
# Printer (check setting anytime printer goes off-line)
# Clock
# MRX parameters
# Maintenance schedule
# Communication parameters -- interface with external computer (see next section)
Creating a New Test
# Test numbers, names and plate ID
# Shaking
# Defining a template
⇒
Well types (B,S,T,C…user-definable)
⇒
Replicates
⇒
Fill (row, column)
⇒
Change
# Wavelength mode (single, dual, multiple)
# Blanking options
# Optical Density (OD) Data Matrix
54 MRX Microplate Reader User Manual
# Data reduction
⇒
Curve-Fitting
⇒
Thresholds
⇒
Ratios
⇒
Differences
⇒
QC equations
⇒
Area statistics
# Password protection
Editing a Test
Running a Test and Recalling Data
Printing -- Data/Tests/Directories
Appendix D: MRX Training Checklist
Utilities
# Manual control of the reader
# Long and short menus
# Version
# Statistical data
⇒
Coefficient of Variation
⇒
Stat istical data for stored plates
# Batch
Spectral Response Curve
Temperature Control
# Safety information
# Features
# Software installation
Setting the temperature
MRX Microplate Reader User Manual 55
Appendix D: MRX Training Checklist
56
Kinetics Software
# Features - values determined from a reaction curve
# Calculation mode
⇒
Endpoint
⇒
Kinetics -- Delta
Total - total rate of change in OD/Min.
Best - greatest rate of change in OD/Min.
Linear - average rate of change in OD/Min.
⇒
Kinetics -- Time
OD (time to a certain OD)
OD/Min (time to a certain rate of reaction)
Relative (time to an OD rel ative to the first OD taken)
⇒
Kinetics -- single well monitoring
⇒
Options
Increasing or decreasing OD
Time intervals
No. of readings
Scaling -- Results Log
⇒
Display a well
# Creating a New Test
⇒
Test
numbers, names and plate ID
⇒
Shaking
⇒
Defining a template
⇒
Wavelength m ode (single, dual, multiple)
⇒
Blanking options
⇒
St art mode
Immediate
OD
OD/Min.
Time
⇒
Data reduction
Curve-Fitting
Thresholds
Ratios
Differences
QC equations
Area statistics
MRX Microplate Reader User Manual
Appendix D: MRX Training Checklist
# Editing a test and ODs
# Running a test and recalling data
⇒
Kinetic output
All ( matrix of plots of 96-Wells)
Zoom (plot of single well)
Row and column plots
Printing -- data/tests/directories
Agglutination Software
# Agglutination mode
⇒
Principles
⇒
Interpreting results
⇒
Calculation modes (Formula and Rules)
# Tissue Culture Growth (TCG) mode
⇒
Principles
⇒
Interpreting results
# Creating a new test
⇒
Test
numbers, names and plate ID
⇒
Shaking
⇒
Filter selection
⇒
Read mode -- Agglutination
Noise filtering
Transmission graphs
Defining a template
Data Log
Calculation mode
Threshold matrix
Recalculating results
⇒
Read mode -- Tissue Culture Growth (TCG)
Plate speed
Readings per well
Defining the template
Blanking
Results matrix
Transmission graphs
Threshold matrix
# Editing a test
MRX Microplate Reader User Manual 57
Appendix D: MRX Training Checklist
Run ning a test and recalling data
Printing -- data/tests/directories
MRX
plus
or MRX
II
from an External Computer Using
Revelation Software
Self-Tests
# Print the self-test
The Utility Menu
# Configure reader
⇒
Internal MRX/External MRX
⇒
Set-up MRX
⇒
Filters
# Spectral Response Curve
# Sample ID entry
# Options
⇒
Quick assay buttons
⇒
Preferences
Automatic data save
Auto print
Plate ID
⇒
Laboratory information
⇒
Directories
# System password
58 MRX Microplate Reader User Manual
Appendix D: MRX Training Checklist
Pull-Down Menus/Tool Bar/Short Cut Keys/Assay
Buttons
# New assay
⇒
Endpoint
⇒
Kinetics
# Open various types of files
⇒
Assays/data/sample IDs/text files/curves
# Importing files
Creating an Assay -- Assay Wizard
# Title/password/bar code
# Reader control
⇒
Read modes
Single/Dual
Multi-Wavelength
Agglutination
⇒
Shake modes
⇒
Start/Stop modes
⇒
Kinetics
Time interval
Calcula tion mode (OD/Min. modes, Time modes, Moving
Average, Cut-Off)
Graphs
# Defining a template
⇒
Well typ es (B,S,T,C…user-definable)
⇒
Replicates
⇒
Fill (row, column, random)
⇒
Change template
# Blanking options
# QC equations for raw data
MRX Microplate Reader User Manual 59
Appendix D: MRX Training Checklist
# Data reduction
⇒
Curve-Fitting
Standards
Fit types and axes
Combine on same graph
Use stored fit
Calculated QC
Data conversion
Output format
⇒
Thresholds
Threshold equations
Segments
Labels
QC
Output format
⇒
Ratios
⇒
Spreadsheet
# Report options
⇒
Report layout
⇒
Data Matrix
⇒
Text file
Options
Open Microsoft
Excel
and import text file
# Assay options
⇒
Area statistics
⇒
Sample IDs
⇒
Processing order
Running a Plate
# Auto save/print/search for sample IDs
⇒
Run a partial plate even though all wells are defined by well type
# Adding assays to the check list
⇒
Multiple assays on a plate
60 MRX Microplate Reader User Manual
Data Menu
# Reprocessing data
⇒
Data refresh
⇒
Select new assay
# Variable parameters
# Reagent lot number
# Outlier removal
Titration multiplier
# Fit information
# Kinetic graphs
Sample ID s
View Menu
Utilities Menu
# Plate-Plate statistics
Temperature Control Option
# Features
# Setting
# Control Log
Barcode Option
# Types
# Features
Appendix D: MRX Training Checklist
MRX Microplate Reader User Manual 61
Appendix D: MRX Training Checklist
Agglutination Option
# Plate speed
# Calculation modes
# Transmission graphs
# Data Log
# Removing readings
# Filtering noise
# Calculating results as a percentage of a specific well
62 MRX Microplate Reader User Manual
Microplate Reader User Manual (105202A)
Index
—A—
Accessories, 45
—B—
Back panel
MRX
II
and MRX
plus
, 12
Back Panel
MRX
Revelation
, 13
—C—
Cleaning, 38
Clock Menu, 24–25
Comms Menu, 28–29
—D—
Decontamination, 38
Display, 15
—E—
Electric Shock Hazard, 3
Equipment in Transit form
(decontamination). See
Appendix B
External Painted and Plastic
Surfaces cleaning the, 38
—F— filter spring positioning, 18
Filters cleaning the, 38
Filters Menu, 23
Front panel, 15
Function keys, 15
MRX Microplate Reader User Manual
I-1
Fuses, 4
—H—
Hardware Specifications, 49
—I—
Incorrect Operation, 3
Keypad, 15
—K—
—L—
Lamp fitting the, 17–18, 17–18 replacing the, 40
—M—
Main Menu, 19
Maintenance, 37–38
Maintenance Menu, 27
Maintenance Reminder, 19, 27
Memory Menu, 29
Microplate Reader. See Reader.
MRX training checklist, 53
MRX front panel, 15
MRX
II
and MRX
plus
back panel,
12
MRX
Revelation
back panel, 13
—O—
Optical filters changing the, 41–42
Optical Filters fitting the, 17–18
63
64
Over Limit, 30
—P—
Param Menu, 30–31
Plate Carrier Diagram, 5
Plate Type, 30
Printer interface, 33–34
—R—
Reader accessories, 45 back panel
MRX
II
and MRX
plus
, 12 back panel
MRX
Revelation
, 13 connecting a printer to it, 33 electrical specifications, 51 features of, 7–8 front panel, 15 hardware specifications, 49 interface, 51 keypad and display, 15 performance specifications,
49–50 physical/environmental conditions, 52 repacking the, 39 resetting its memory, 29 self-tests, 19 software specifications, 49 switching it on, 18 unpacking the, 11
Repacking the Reader, 39
Repeatability Mode, 31
Resetting the Reader memory, 29
—S—
Safety Information, 3–4
Self-tests, 19, 27, 43 sample printout, 44
Setup menu, 21–22
Specifications electrical, 51 hardware, 49 interface, 51 performance, 49–50 physical/environmental, 52 software, 49 switching between DOS applications, 20
System Menu, 25–26
—V—
Voltage Selector, 14
—W—
Warning Symbol, 5
Windows QWERTY Keyboard
Option, 16
MRX Microplate Reader User Manual
Microplate Reader User Manual (105202A) I-1
MRX Microplate Reader User Manual 65
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