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Thermal Cycler
CG Palm-Cycler
Gradient Thermal Cycling
Tel: +91-11-4502-7000 Email: [email protected] Website: www.genetixbiotech.com
The new Gradient Palm-Cycler combines robust
engineering advanced performance and a palmtop
PC interface for supreme user-friendly flexibility.
Features
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Active-cooled block (range 4°C to 99°C)
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Allows total flexibility in cycle programming
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8 Peltiers and 4 platinum sensors
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SD memory cards for program transfer & backup
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True linear gradient capability (1°C to 24°C)
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Intuitive Palm computer interface
Computer supports advanced software (for
autocalibration, primer design, virtual runs, etc)
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Easy programming and backup
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Advanced cycling options (Touchdown, Long Range,
Ramp Rate, Hold List, etc)
Intuitive graphical user interface with large
backlit, color, touch-sensitive screen
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Advanced functions (graphing interface, autocalibration,
oligo calculator etc)
In-built palm calendar software makes a perfect
lab booking system!
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It is an indigenous product
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Built tough to last
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Oligo Calculator Software
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Familiar Windows operating system
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01
Thermal Cycler
Palmtop PC Interface
A palmtop PC interface provides the
convenience of the familiar graphical
windows® operating system
combined with a backlit color
touchscreen for an unsurpassed user
experience in a thermal cycler.
The power of a palmtop PC
computer allows advanced software
functions to be included and total
flexibility in thermal cycle
programming new software
features are regularly incorporated
and available as free upgrades via web
download.
An SD memory card is included for
convenient backup and transfer of
programs between instruments. Each
card can hold thousands of saved cycle
programs.
In addition, the calender software
included with the palmtop PC
computer makes a perfect booking
system for laboratories where the
instrument is in high demand!
True Linear Gradient
Thermal gradient technology
enables opposite sides of a thermal
cycler block to be set to a different
temperature. The wells across the
block then span the set temperature
range, creating a thermal gradient.
A gradient can be used, for example,
to optimize the annealing
temperature of an assay in a single
experiment by simply determining
the temperature of the wells that
yielded the best result.
Tel: +91-11-4502-7000 Email: [email protected] Website: www.genetixbiotech.com
The Palm-Cycler® uses eight Peltier
devices to actively heat and cool the
block from 40C up to 990C. Peltier
devices are controlled by four
separate thermal sensors arranged
across the block. The distribution of
sensors enables a true linear
gradient to be established via
feedback loops from the sensors to
the computer. Furthermore, Palm-
Cycler software can
interpolate and display the
temperature of each
column of wells in real-time
during a run.
With the Palm-Cycler, a
linear gradient of between
10C and 240C can be set
across the block.
Unlike the Palm-Cycler,
other thermal cyclers
typically use fewer Peltier
devices ad sensors. A
thermal gradient produced by these
instruments can be very non-linear,
having a fast thermal decay zone at
one or more regions of the block. An
asymmetric gradient such as this
makes it very difficult to predict actual
sample temperature in each well and
makes optimization experiments
prone to error.
Thermal Gradient Cycling
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When enabled, a programmable gradient from 1°C to 24°C can be setup
from left to right on the Palm-Cycler
8 Peltiers and 4 sensors enable a true linear gradient to be established
Gradient technology allows the user to quickly identify the best possible
set of annealing conditions in one run when optimizing cycling conditions
Shortly, Palm Cycler
will be available with
new “Pharos” Palm Top
01
Thermal Cycler
Support for all Reaction formats
There are three models of Palm-Cycler to suit different sample tube formats:
Industry Standard 384-well format for
plates with adhesive seals.
Industry Standard 96-well format for
0.2ml individual or strip tubes
(domed or flat - capped) or 96-well
plates (low or high skirt) with strip
caps or adhesive film seals.
Real-time Graphs
Programming Interface
Palm-Cycler software graphically displays the current
measured temperature across the block in real-time as cycling
progresses, even for a thermal gradient (A opposite). Blue
lines on the graph indicate target temperatures for each
thermal sensor and the red lines report the measured
temperature at each sensor location.
Cycling programs are created and edited in
Program mode. A program is graphically
represented, as shown opposite for a three step
program (A). The touchscreen interface makes
the Palm-Cycler simple and intuitive to use:
displayed parameters such as temperature, time,
step number, or repeat number can be changed
simply pressing on a displayed value to open an Edit
dialog (B opposite).
Four sensors across the Palm-Cycler block enable accurate
interpolation of the temperature in each well. Shown
opposite (B) are the interpolated temperatures of all twelve
columns across a 96-well block (i.e. 12 columns x 8 rows) as
displayed in real-time by the software.
A
Ramp Rate Control
B
Palm
PalmCycler
Cycler
X
7:17
Elapsed: 0:06:29
Stop
Estimated Total Time: 2:02:12
Thermal transition times (or ramp rates) can be
precisely controlled by the Limit Rate function.
Limit Rate allows the user to specify the time
taken to transition from one set temperature to
the next.
Stop
80°
70°
1: 60.0°C
2: 58.0°C
50°
3: 56.1°C
4: 54.2°C
40°
5: 52.4°C
6: 50.8°C
30°
7: 49.2°C
8: 47.5°C
9: 45.7°C
10: 43.8°C
11: 41.9°C
12: 40.0°C
60°
10°
0:32
2:00
4:00
6:28
File Control Options Help
File Control Options Help
A
The Hold function allows the creation of one or more pauses
in the program. With this feature active, the unit will pause
at the programmed point while emitting an alert beep. The
set temperature will be held indefinitely until a Continue
button is pressed. holds are useful if there is a need to
remove tubes or add reagents at a particular point in a run.
X
Column Temperatures
90°
20°
Hold Lists
7:17
Palm
Palm Cycler
Cycler
Cycle 1 of 2 (repeat 2 of 30)
profile : Untitled
Tel: +91-11-4502-7000 Email: [email protected] Website: www.genetixbiotech.com
60-well format to suit large volume
reactions in individual 0.5ml tubes
with attached caps
B
7:17
Palm
PalmCycler
Cycler
<- (1)
Repeats
Steps
X
-> (+)
0:30
72.0°C
55.0°C
0:30
3:00
ok
:30
Cycle 2 of 2 (40 repeats)
Profile: Untitled
95.0°C
7:14
Palm
PalmCycler
Cycler
Desired duration:
7
8
9
4
5
6
1
2
0
3
OK
Clear
Cancel
TouchDn:N LongRng:N
File Control Options Help
01
Thermal Cycler
Manual Control
Zero-condensation Heated Lid Design
In Manual Mode, the Palm-Cycler block can be set to hold
a specified temperature for an indefinite time. This
function is useful for incubating reactions such as DNA
digestion or ligation. Simply select Manual Mode from the
control menu and key in the temperature desired.
Manual Mode also supports a thermal gradient in which
the upper and lower temperatures are set.
The Palm-Cycler uses a heated lid to keep the void air
volume in the tube hotter than the reaction (see below).
This drives any evaporation and condensation back into
the cooler reaction liquid. Thus no oil or wax
condensation barrier is needed.
Heated Cover
Touchdown And Long Range
Touchdown and
Long Range are auto-increment
programming tools supported by the Palm-Cycler.
Touchdown allows the temperature of a step to be
automatically lowered by a set amount over a specified
range of successive cycle repeats. It is usually used as a
mechanism to avoid primerdimer artifacts by gradually
lowering the annealing temperature during early cycle
repeats of an amplification reaction.
Long Range programming enables the time of a particular
step to be automatically increased by a set amount over a
specified range of successive cycle repeats. Long Range
programming is often used to provide gradually extended
times for enzymatic polymerization of longer products
during later cycle repeats of an amplification reaction. It is
most useful for targets longer than about 1kb.
Oligo Calculator
A
Tel: +91-11-4502-7000 Email: [email protected] Website: www.genetixbiotech.com
Palm
Palm Cycler
Cycler
B
01
7:22
X
Palm-Cycler software
includes a full-featured
oligo calculator as an aid to
oligonucleotide design. In
addition to a range of
calculated values, the
software self-aligns the
oligo to show the
occurrence of selfcomplimentary bonds. This
can be used, for example, to
identify palindromes and
primer-dimers. Example
oligo information is shown
here (A). Scrolling the
window down displays the
self-aligned bond
information ( B).
PCR Tube
Reaction Mix
Aluminum Block
Peltiers
Heated Lid (Hot Bonnet) Technology
Thermal Calibration
In some situations it may be desirable to have the PalmCycler matched to an external thermal accuracy standard.
The software includes an auto-calibration wizard in the
Help menu to enable dynamic recalibration by the user
The calibration process begins with measurement of
Palm-Cycler well temperatures using a thermocouple
probe of the desired accuracy. Measured temperatures are
then used to overwrite existing Palm-Cycler calibration
settings so that accuracy is matched to the probe.
Power Interruption and Auto-resume
If for any reason the Palm-Cycler is turned off, the power
fails or the palmtop PC is disconnected during a run the
palmtop PC will sound a warning alarm for 30 seconds. If
reconnection occurs during that time the program will
continue. If not, a Disconnected message appears
onscreen indicating the name of the terminated program
and the cycle, step and repeat number at which
interruption occurred.
Thermal Cycler
Technical Specifications
Thermal Cycling System
Active heating and cooling using 8 Peltier elements + 4 Platinum sensors
Temperature Range
40C-990C
Temperature Accuracy
±0.250C of set temperature, 1 minute after clock start
Temperature Uniformity
±0.50C, 30 seconds after clock start
Temperature Resolution
0.1 C increments
Heating/Cooling Rate
2.50C/sec maximum
0
Condensation control
Automatic using a heated lid design
Special Functions
Auto-resume on power fail, on-line logging and graphing, touchdown and long-range
programming, linear thermal gradient (programmable up to 240C), adjustable ramp
rate, oligonucleotide calculator, program holds, manual mode, online help, thermal
calibration wizard.
Configurations
60-Well version (CG 6000-000)
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Supported consumable: 0.5 mL Tubes with attached caps
96-Well version (CG 9600-000)
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Supported consumables: 0.2 mL tubes or strip tubes with flat or domed caps; 96-well
high-or low-skirt plates with strip caps or an adhesive cover
384-Well version (CG 3840-000)
Dimensions
Supported consumables: 384-well plates with an adhesive cover
H 220 mm (8.7”) lid closed
H 330 mm (13.0”) lid open
W 310 mm (12.2”)
10.9 kg (24 lbs)
Electrical
100-120 VAC @ Amp (50/60 Hz), 200-240 VAC @ 2 Amp (50/60 Hz)
Startup Accessories
Blue Sillicon Pad. Provided with 96- and 384-well configurations only Secure Key for Palm
Device.
Tel: +91-11-4502-7000 Email: [email protected] Website: www.genetixbiotech.com
D 295 mm (11.6”) ; 370 mm (14.6”) including cables
Weight
SD Memory Card (2 GB)
Power Cable
User Manual
Palmtop PC accessories (manufacturer’s standard set)
Software
Supplied, with unlimited user license.
Warranty
1 years on instrument, manufacturers warranty on palmtop PC device
Ordering Information
Cat #
Description
CG6000-000
CG Palm Cycler 60 well version ; supported consumables (0.5 ml tubes with attached caps)
CG9600-000
CG Palm Cycler 96 well version ; supported consumables (0.2 ml tubes or strip tubes with
flat or domed cap 96-well high - or low - skirt plates with strip caps or an adhesive cover
CG3840-000
384 well version ; supported consumables,384-well plates with an adhesive cover
Price (`)
01
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