routines at the department of pharmacy

routines at the department of pharmacy
HSE
Health, Safety and the Environment
SCHOOL OF PHARMACY
HSE MANUAL
07.04.2011
School of Pharmacy HSE manual: Contents
CONTENTS
INTRODUCTION .......................................................................................................................................3
FIRST AID RULES .....................................................................................................................................8
FIRE INSTRUCTIONS............................................................................................................................. 11
COMMON ROUTINES ............................................................................................................................ 14
ROUTINES AT THE ADMINISTRATION ............................................................................................. 35
ROUTINES AT THE DEPARTMENT OF PHARMACY....................................................................... 37
ROUTINES AT THE DEPARTMENT OF PHARMACEUTICAL BIOSCIENCES ............................. 50
ROUTINES AT THE DEPARTMENT OF PHARMACEUTICAL CHEMISTRY ............................... 94
APPENDICES ......................................................................................................................................... 140
LIST OF TELEPHONE NUMBERS AND ADDRESSES ..................................................................... 148
2
School of Pharmacy HSE manual: Introduction
INTRODUCTION
PREFACE
The University in Oslo, and the Faculty of Mathematics and Natural Sciences, HSE Manuals apply to the
School of Pharmacy. The HSE Manual for the School of Pharmacy therefore covers special regulations that
apply exclusively to the School, as well as information that is useful for HSE work here.
The Manual contains an organisation chart displaying the responsibility and decision-taking structure in HSE
work at the School. Conditions relating to Local Working Environment Committees are described, and
references are given to general laws and regulations in the applicable health, safety and environment
legislation.
The HSE Manual contains a chapter for each department in the School and for Administration.
The HSE Manual will be updated as required and as agreed with the secretary of the Local Working
Environment Committee [L-AMU]. All employees (and students) are responsible for staying updated on the
HSE regulations applicable at any time at the School.
School Of Pharmacy
19.12.2008
HSE GOALS AT THE SCHOOL OF PHARMACY
The School of Pharmacy endorses the HSE goals of the University of Oslo (UiO):
In order to achieve its goal of being a leading research university, UiO must also be at the forefront within
HSE, with hazards under control, and experiences under continuous development.
Hazards: under control - Experiences: under continuous development
This can be achieved by working towards:
- Working environments for employees and learning environments for students that are completely safe
and nurturing
- An organisation that is action-taking, including, and learning
- Buildings that are accessible, functional, and safe
- Operations that are completely safe for the exterior environment
LEGISLATION
A few central laws that apply to HSE (for additional laws, refer to the HSE Manual for the Faculty of
Mathematics and Natural Sciences):
- “Arbeidsmiljøloven [The Working Environment Act]”
- “Lov om tilsyn med elektriske anlegg og elektrisk utstyr [Law of 24 May, 1929 regarding the
supervision of electrical installations ]”
3
School of Pharmacy HSE manual: Introduction
- “Lov om vern mot brann, eksplosjon og ulykker med farlig stoff og om brannvesenets
redningsoppgaver (brann- og eksplosjonsvernloven) [Act relating to the prevention of fire, explosion
and accidents involving hazardous substances and the fire service (The Fire and Explosives Act) ]”
- “Forurensningsloven [The Pollution Control
- Act]”
- “Genteknologiloven [The Gene Technology Act]”
- “Strålevernloven [Act on radiation protection and use of radiation ]”
4
School of Pharmacy HSE manual: Introduction
RESPONSIBILITIES AND DECISION-TAKING STRUCTURE IN HSE ISSUES
In general, an attempt shall be made to resolve HSE-related matters at the lowest possible level in the line, in
order to achieve local participation. Responsibility follows the line from the Director, School of Pharmacy to
the Head of Department.
The immediate line manager at each level is responsible for ensuring that the instructions are known and
complied with. The immediate line manager is a scientific employee in a permanent position with
responsibility for own research and/or as student advisor for Master students, research fellows, guest
researchers, or who uses technical personnel in their research.
Instituttdirektør
Administrasjonen
Studie/ Økonomi/ IT
Avdeling for
farmasøytisk
biovitenskap
Avdelingsleder
Avdeling for
farmasi
Avdelingsleder
Avdeling for
farmasøytisk kjemi
Avdelingsleder
Director of the School of Pharmacy
Administration
Studies/Finances/IT
Department of
Pharmacy
Head of Department
Department of
Pharmaceutical
Biosciences
Head of Department
Department of
Pharmaceutical Chemistry
Head of Department
LOCAL WORKING ENVIRONMENT COMMITTEE (L-AMU)
SIZE AND COMPOSITION
- Employer representatives (2):
Director, School of Pharmacy and one employer representative elected by management.
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School of Pharmacy HSE manual: Introduction
- Employee representatives (2):
Head Safety Delegate and one employee representative elected by the employees
- Employer representative for student matters (1):
Secretary of L-AMU
- Student representative (1):
One student elected by the Pharmacy Subject Committee.
WORK TASKS
- L-AMU is responsible for updating the School's HSE Manual
6
School of Pharmacy HSE manual: Introduction
HSE MANUALS
The HSE Manuals for UiO, the Faculty of Mathematics and Natural Sciences, and the School of Pharmacy
apply to everyone on the premises of the School of Pharmacy. A declaration confirming familiarity with the
HSE Manuals and signed by the employee must be archived in the Department. A declaration signed by
students undertaking a laboratory-based master thesis, is to be archived with the student advisor responsible
for the 2nd department.
HSE TRAINING
The HSE course run by the Faculty of Mathematics and Natural sciences is compulsory for all employees.
Net-based laboratory safety courses are compulsory for all Master thesis students.
The person responsible for the substances and chemicals register can provide training in the use of the netbased substances and chemicals register (HSE datasheet).
All types of training and times are listed on the School's HSE web page.
HSE WEB PAGES
UiO HSE web page: http://www.uio.no/for_ansatte/ansatt/arbeidsmiljo/
Faculty of Mathematics and Natural Sciences web page:
School of Pharmacy HSE web page: http://www.farmasi.uio.no/ansatte/HMS.html
FORMS
Forms can be found via the School of Pharmacy’s HSE web page (or in the appropriate HSE Manual).
7
School of Pharmacy HSE manual: First aid rules
FIRST AID RULES
GENERAL RULES IN THE EVENT OF INJURY
Immediately inform a member of staff.
In the event of a chemical accident, you must report the chemical(s) involved.
SPLASHES IN THE EYES
Rinse continuously with water, preferably from an eye douching bottle.
Continue rinsing while en-route to the doctor and while waiting at Ullevål University Hospital until seen by a
doctor in the Ophthalmology department (Remember to take additional eye douching bottles with you).
SPILLAGE ON SKIN (AND CLOTHES)
Rinse with water for at least 15 mins. Wash with soap and water. Remove soiled clothes, shoes, and jewellery.
Skin contact with wet clothes can often cause worse injury than spills directly on the skin.
(Therefore it is important to change disposable gloves frequently.)
SWALLOWING CHEMICALS





Drink plenty of water (dilution effect). Get medical help.
Call the Norwegian Poison Information Centre (Giftinformasjonen) (tel. 22 59 13 00).
If organic solvents, hydrocarbons, etc. are swallowed, give fats such as cooking oil, milk, cream, or
ice-cream.
If organic solvents or corrosive liquids are swallowed, the person must not vomit
as the fluids can enter the lungs and result in chemical pneumonia.
NB! Do not give food or drink if the person is unconscious.
If NaOH (sodium hydroxide) is swallowed, give milk if possible.
Emetic: 2 dessert spoons of table salt (NaCl, sodium chloride) in a lukewarm glass of water.
INHALATION OF POISONOUS GAS, VAPOUR, OR DUST

Fresh air and complete rest. Contact the doctor/ambulance.
If respiration stops, give artificial resuscitation (mouth-to-mouth).
WOUNDS FROM GLASS OR SIMILAR

Wash the wound thoroughly with cold water to remove any possible contamination and to cool the
skin so that the blood vessels contract. Try to stop/reduce the bleeding by applying a plaster/sterile
bandage. If the wound is large or relatively deep, contact A&E (to check whether stitches are
necessary).
BURNS

Cool immediately and continuously in cold water for at least 20 mins,
and continue during transport to the doctor.
ELECTRIC SHOCK

Strong blow to the chest, if the heart has stopped. If necessary, immediately commence heart massage
and artificial respiration (mouth-to-mouth resuscitation).
FAINTING

Ensure the airways are free, place the person in a comfortable position (stable side position), loosen
any tight clothes, and check the pulse and breathing. As necessary: Cardiopulmonary resuscitation
with heart massage and artificial respiration (mouth-to-mouth resuscitation).
SHOCK

8
Try to calm the injured person. The injured person should preferably lie or sit comfortably with their
feet above the level of their head. Make sure they have fresh air and are comfortably warm.
School of Pharmacy HSE manual: First aid rules
ACCIDENTS INVOLVING BIOLOGICAL MATERIALS
Spillage of cultures or other biological material.
Spills must be disinfected immediately. Using gloves, remove the spillage using paper wetted with 70%
alcohol. Wet the dried surface with 70% alcohol and leave to stand for a few minutes. Wipe dry. Dispose of
the paper and gloves in a hazard waste box.
ACCIDENTS WITH CHEMICALS (SOME SPECIFIC)
In general, refer to the safety data sheets in the Substances and Chemicals Register ECOonline. See the
dedicated section on Safety datasheets and the Substances and Chemicals Register under The Laboratory:
Safety and the Working Environment.
BROMINE (Br2) ON SKIN

Rinse the skin with plenty of sodium thiosulphate solution (Na2S2O3).
Afterwards, rinse with 60% ethanol and polyethylene glycol (Or use pyrisept ointment with
polyethylene glycol as the ointment base.)
The dangerous bromine (Br2) is reduced by the thiosulphate (S2O32-) to the relatively harmless
bromide (Br -).
ETHIDIUM BROMIDE
This chemical (crystals and solutions) is a potent mutagen

If inhaled or swallowed, seek medical advice.

If in contact with the eyes or skin, wash for many minutes with water.

In the event of spillage, wash with 1-2% Deconex.
PHENOL (C6H5OH) ON SKIN

Rinse with water. Next, wash the skin repeatedly with glycerol or aqueous polyethylene glycol (PEG).
Apply pyrisept ointment liberally to the skin.
MERCURY (Hg) SPILLAGE (ON WORKBENCHES/FLOORS)

Draw up the metallic mercury using an eye dropper into a container.
Spread sulphur powder (S) over the contaminated area (Hg + S = HgS). Leave for a short while before
collecting everything together in a container. (Mercury absorbent can be used instead of sulphur (S)).
The container is to be disposed of as chemical waste (Heavy metals, mercury).
ACCIDENTS WHEN WORKING WITH RADIOACTIVE SUBSTANCES
All employees who work with radioactive substances in the department must complete “Kurs i strålevern og
radioaktivitet” [Course in radiation prevention and radioactivity) (Department of Chemistry, UiO) before start
of the work. Other than this, there is daily instruction in work routines in the laboratory through personal
instruction (e.g. higher degree students).
PRACTICAL MEASURES IN THE EVENT OF AN ACCIDENT
In the event of an accident with radioactive substances, measures must be initiated promptly to limit spread
and unnecessary exposure. The contaminated area must be cordoned off and access must be blocked.
Protective gear must be used (gloves, coats, disposable shoecovers, and measuring devices). If equipment
and/or workplace have been contaminated, the decontamination procedure must be performed. Washing must
be carried out for example with 5% Deconex, preferably several times. If it is not possible to remove the
contamination in this way, the HSE department must be notified. A contaminated surface that cannot be
decontaminated must be removed, or screened if necessary. Contaminated clothes must be changed and
9
School of Pharmacy HSE manual: First aid rules
washed (separately). Contaminated skin must be washed immediately with soap and water. All accidents with
radioactive substances must be notified immediately to the responsible persons in the department (Safety
delegate, Radiation Safety Coordinator and Head of Department).
10
School of Pharmacy HSE manual: Fire instructions
FIRE INSTRUCTIONS
Meeting place: Between the School of Pharmacy building (BL 25) and Helga Engs building (BL20)
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School of Pharmacy HSE manual: Fire instructions
FIRE INSTRUCTIONS FOR USERS
The University of Oslo
Fire Instructions for Users
All users of the University buildings have a responsibility to:

Conduct their activities in such a way that fire does not easily breakout
To reduce the fire hazard it is forbidden to use candles and other forms of open flames in
all University buildings, with the exception of controlled work in laboratories, churches,
and on staffed catering units where the restaurant has a license to sell alcohol.




Familiarise themselves with the fire safety measures in the building. Take particular note of the
location of fire exits, fire extinguishing equipment, and fire alarms.
Not damage the fire safety measures that have been implemented. This applies in particular to fire
exits, fire alarm installations, emergency lighting, fire extinguishing equipment, fire walls, and fire
doors.
Report anything that is faulty or missing to the person responsible for that section/floor.
Report all damage/accidents and near-accidents that have, or could have, led to fire, or outbreak of
fire. This must be reported on the standard form on the web pages of the Section for HSE.
All users have a duty to familiarise themselves with the fire safety measures in the building. This applies
in particular to the location of fire exits, fire extinguishing equipment, and fire alarms.
In the event of a fire alarm:


Follow the general fire instructions
Leave the building through the nearest fire exit and, once outside, do not stand by the entrance!!
I have read and understood my instruction as a user of the buildings at the University of
Oslo (UiO).
Building/Section: ______________________________________________
_______________
Date
12
___________________________________
User
School of Pharmacy HSE manual: Fire instructions
FIRE INSTRUCTIONS FOR FIRE SAFETY SECTION LEADERS
THE UNIVERSITY OF OSLO
Fire instructions for floor and/or section leaders
Floor and/or section leaders are responsible for:







Knowing the general fire instructions, the instructions for abnormal and varying risk, and local fire
instructions, if applicable.
Familiarising employees and students with fixed workplaces on their floor/section with the general
fire instructions and the user instructions. In addition, all escape routes and technical fire measures
must be demonstrated.
When this information has been received, this document must be signed by the recipient and then
sent to the Head of Unit.
Providing new employees with the information above within the first week of employment. This
must be documented in the same way as above. This also applies to temporary employees, temps,
and consultants who it is anticipated will work on that floor for more than 1 week.
Routinely checking the emergency escape routes and other technical fire measures in their area of
responsibility. If any faults or deficits are found, these must be reported to the Fire Safety Officer
with a copy for the Head of Department. Forms for these reports can be found in the fire safety
documentation and on the Technical department’s webpage. Electronic mail may also be used.
Knowing the content of the fire safety documentation and where it is located.
Informing the Head of Department of people with disabilities on their floor/in their department.
Cooperating with the Head of Department, Fire Safety Officer and Safety Delegate to ensure the
satisfactory safety of people with disabilities in the event of a fire.
Familiarising at least one deputy with these instructions.
In the event of a fire alarm:




Put on the fire safety vest and encourage people on your floor/in your department to leave the
building.
Leave the building and ensure that people do not stand right outside the entrance!!
Notify the person in charge at the Fire Services of any people who have not left the building and
where they may be found.
Inform users on your floor/in your department of the cause of the fire alarm.
These tasks may be delegated, but the overall responsibility remains with the floor/department leader.
I have read and understand the instructions for floor/department leader
Building/floor/unit: _________________________________________
_________________
Date
____________________________
Floor and/or department leader
13
School of Pharmacy HSE Manual: Common routines
COMMON ROUTINES
ACTION PLAN IN THE EVENT OF SERIOUS ACCIDENTS, INCLUDING
MANDATORY NOTIFICATION TO THE NORWEGIAN LABOUR INSPECTION
AUTHORITY
PURPOSE
To ensure that necessary first-aid is given, and to ensure that the correct units are notified in the event of
serous personal injury (including notification to the Norwegian Labour Inspection Authority). To prevent new
accidents and injury.
RESPONSIBILITY
The immediate line manager at the site of the accident/incident is responsible for following up the
accident/incident both with regard to the injured and with regard to notification on the appropriate form.
DEFINITION



Serious accident: An undesired incident that results in serious injury to people, the environment, or
materials.
Accident: An undesired incident that results in minor injury to people, the environment, or materials.
Near-accident: An undesired incident that in slightly different circumstances would have resulted in
injury to people, the environment, or materials.
PROCEDURE
ACTION PLAN IN THE EVENT OF SERIOUS ACCIDENTS/INJURY
1. Give first aid. UiO Health Services (Helsetjenesten) (tel. 22 85 31 74)
2. Notify the Security Centre at UiO (tel: 22 85 66 66) who will forward emergency calls as appropriate to:
- Fire Services (tel. 110), Police (tel. 112), Oslo A&E services (tel. 113)
- UiO Health Services 22 85 31 74
- Ullevål University Hospital: A&E Reception (tel. 22 11 73 50)
- Ullevål University Hospital: Department of Ophthalmology (tel. 22 11 85 45/22 11 85 47) The
Norwegian Poison Information Centre (tel. 22 59 13 00)
- Taxi – emergency help (tel. 22 38 80 50)
3. Immediately notify the accident by phone (Head of Department or their deputy) to:
- The Safety Delegate
- The Director, School of Pharmacy (tel. 22 85 6586) (who forwards the information to the Faculty
Administration)
- Section for HSE at UiO (tel. 22 85 88 95)
- The Norwegian Labour Inspection Authority 2nd District (tel. 23 08 05 05, fax: 22 17 78 10)
4. Secure the site of the accident, if possible, until the police/Norwegian Labour Inspection Authority
arrives.
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School of Pharmacy HSE Manual: Common routines
POST-INCIDENT ACTIONS IN THE EVENT OF ACCIDENTS /NEAR-ACCIDENTS
5. All accidents/near-accidents must be recorded on internal accident notification forms and sent to the
Director, School of Pharmacy with a copy to the HSE Section at UiO (no later than 3 days after the
accident).
(The Director, School of Pharmacy must archive a copy in the injured person’s employee folder)
The internal accident notification form minus personal details is sent to the Head Safety Delegate at UiO.
6. In the event of serious accidents or as requested, supplementary information on the course of the incident
must also be sent to the Director, School of Pharmacy, the HSE Section at UiO, and the Norwegian
Labour Inspection Authority 2nd District.
7. The Director, School of Pharmacy reports an injury to the injured person’s Social Security Office (RTV
Form IA 13-07.05), when indicated by the injury and always when:
- the injured person has received medical treatment
- the injury results in incapacity
- the injured person wishes this
- the Social Security Office requests this
The notification must be sent as soon as possible and no later than 3 days after the incident. Remember to
report all injuries. Seemingly minor injuries can develop into serious problems with major economic
consequences.
8. The injured person must contact their local Social Security Office/Pension Fund for reimbursements.
Reimbursement is conditional on notification of the injury to the appropriate body.
9. Consent must be obtained from the injured person to enable use of the personal details from the accident
in internal accident prevention efforts (The Working Environment Act §20). If consent is not given, the
form must be stored confidentially in the injured person’s company medical records.
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School of Pharmacy HSE Manual: Common routines
REGISTERING ACCIDENTS AND NEAR-ACCIDENTS
PURPOSE
To secure the rights of the injured person(s) when an undesired incident has occurred.
To prevent new accidents and injury.
RESPONSIBILITY
The immediate line manager at the site of the accident/incident is responsible for following up the
accident/incident both with regard to the injured and with regard to notification on the appropriate form.
DEFINITION OF INJURY, ACCIDENT, AND NEAR-ACCIDENT
The form for “Internal Notification of Injury” must be used in the following injuries/accidents and nearaccidents:

Accidents that result in absence or medical treatment

All injuries or accidents that require first-aid

All material damage and/or environmental damage (fire, chemical spill, etc.)

All undesired incidents/dangerous conditions that could have resulted in harm to persons and/or
materials and the environment (near-accidents)

All near-accidents
PROCEDURE
1. All personal injury, accidents and near-accidents are notified on the form for “Internal Notification of
Injury”. The immediate superior at the incident site is responsible for completing and distributing the
form. One (1) form must be used per person.
2. A copy of the completed form must be sent to the Head of Department for the involved department, the
Director, School of Pharmacy, and the HSE Section (PO 1071 Blindern).
3. Reports are processed continuously at the School’s L-AMU meetings, and in the department in which the
injury occurred.
4. The School’s L-AMU- meetings undertake a six-month audit of all notified injuries/accidents and near
accidents, and in particular assess whether preventive measures are to be instigated.
5. If the scope of the incident is greater than that outlined above, the routines for mandatory notification to
the Norwegian Labour Authorities of accidents in the workplace with serious personal injury must be
followed.
6. For all personal injury requiring medical assistance, sick leave and for injuries that could result in future
incapacity even if medical assistance is not called, the immediate line manager must ensure that “RTV
Form 13-07.05” is sent to the Norwegian Labour and Welfare Administration. The form can be obtained
from the Social Security Office or the HSE Section, PO. 1071 Blindern.
7. The Norwegian Labour and Welfare Administration’s “RTV Form 13-07.05” must also be sent to the
State Pension Fund if the employee is a member there, in accordance with the guidelines in section 5.
8. Consent must be obtained from the injured person to enable use of the personal details from the accident
in internal accident prevention efforts (The Working Environment Act §20). If consent is not given, the
form must be stored confidentially in the injured person’s company medical records.
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School of Pharmacy HSE Manual: Common routines
THE LABORATORY: SAFETY AND THE WORKING ENVIRONMENT
ROUTINES FOR HANDLING AND STORING CHEMICALS AT THE SCHOOL OF
PHARMACY, UNIVERSITY OF OSLO
Version: 17.04.2009 P. Rongved
PURPOSE
The routines describe the general guidelines for handling and storing chemicals including in-house
manufactured mixtures/solutions. Storage in this instance is the storage of chemicals that are not for
immediate use. Correct storage of chemicals is necessary in order to reduce the potential for chemical impact
on the environment, and to reduce the extent of harm if an accident should occur. Handling in this instance is
the handling of chemicals during use in relation to HSE, as well as routines for handling chemicals that are to
be destroyed.
1
DEFINITIONS
Term
Incompatible chemicals
Secondary containment
Explanation
Chemicals that react with each other and form: Poisonous or explosive
compounds, gasses, or heat. For example: ox./red. agents; acids/bases and
combinations of these; cyanide salts together with acids.
Secondary containment: Containers/vessels that capture spills if the primary
container is broken. The container must be made from resistant materials.
Flashpoint
The temperature at which a fluid in a given flashpoint device at a certain air
pressure produces so much vapour that, at ignition, a flame occurs above the
surface of the liquid.
Fire cell
Entire building or sections of a building in which a fire can freely develop
without spreading to other buildings or other parts of the building within a
given time.
Flashpoint < 23°C
Flashpoint between 23°C - 55°C
Flashpoint above 55°C
Class A liquid
Class B liquid
Class C liquid
2
RESPONSIBILITY
Head of Department is responsible for ensuring that the routines are known, implemented, and
complied with.
 Each department must have a Technical member of staff who is responsible for handling chemicals and
maintaining a chemicals register. Employees and managers who use the chemicals are obliged to
follow the routines.

17
School of Pharmacy HSE Manual: Common routines
3
PROCEDURES FOR USE AND STORAGE OF CHEMICALS
3.1 GENERAL
All chemicals must have a dedicated storage location, separate from the workplace, when not in use. The
storage location must be tidy and clearly set out. The storage location and chemical containers must be marked
with the correct hazard symbol. Detailed information on the requirements for the individual chemical’s
storage conditions and labelling can be found in the chemical HSE datasheet, which must be registered in the
School’s database (currently ECOonline). Chemicals removed from the chemical stores that are not in use
daily must be returned to their dedicated storage location.
Chemicals that are no longer used and chemicals whose shelf-life has expired must be removed preferentially
from the storage location and handed in for destruction (see section 5). Nevertheless, these chemicals can
continue to be used for technical tests after assessment for safety and quality by chemists with the appropriate
competence.
3.2 GENERAL REQUIREMENTS FOR STORAGE ROOMS
Storage rooms must have floors, walls and ceilings made from resistant materials. The room must be dry and
well-ventilated. Emergency showers, eye douches, adsorption agents, and fire-extinguishing devices of the
correct type must be located in the immediate vicinity of the storage room. The storage room must be
inspected regularly. The storage room must be marked in accordance with the regulations on safety signs and
signals in the workplace.
3.3 GENERAL SAFETY REGULATIONS
In general, chemicals must be stored on the “first in - first out” principle to avoid exceeding their expiry date.
If the chemicals do not have a date stamp on the packaging, the date of reception must ALWAYS be noted on
the chemical before placing in stores.
3.4 INCOMPATIBLE CHEMICALS
Incompatible chemicals must be stored apart in a dedicated storage facility. A dedicated storage facility is an
arrangement that prevents contact between the chemicals in the event of leakage. Small amounts of
incompatible chemicals (<100 ml) may be stored in the same facility if a risk assessment has been conducted
and suitable secondary containment is used.
3.5 FLAMMABLE SUBSTANCES, LIQUIDS, AND GASSES
Flammable substances, liquids, and gasses must be stored in fireproof cabinets or rooms. The cabinet or room
must be marked with a fire hazard sign. The container for storing flammable goods must be in a secure
location. Accessibility must be facilitated in connection with fire-fighting, status checks, and maintenance.
Flammable gasses must not be stored in attics or basements due to the seasonal variation of the outside
temperature combined with a lack of inside temperature control.
3.6 FIREPROOF CABINETS/FRIDGES IN LABORATORIES
In laboratories, flammable substances must be stored in dedicated fireproof cabinets. The cabinets must have a
fire-resistance of at least 20 minutes. The cabinets must be ventilated, and when scrapping old cabinets these
must be replaced by ventilated cabinets. The doors must always be closed when not in use.
Fridges that are used to store flammable substances must be specially designed and constructed for this
purpose.
Fireproof cabinets and fridges which are used to store flammable substances must be marked with the correct
hazard signs (see Appendix in the HSE manual).
A laboratory with a closed door constitutes a fire cell. Up to 40 litres of Class A liquids may be stored here.
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School of Pharmacy HSE Manual: Common routines
3.7 SUBSTANCES WITH SEVERAL HAZARD CLASSES
Some chemicals come under several hazard classes. In such cases, the hazard classes are ranked as follows,
with the greatest hazard listed first:
1. Flammable substance
2. Oxidising substance
3. Acids
4. Bases
5. Miscellaneous
Chemicals that are both flammable and oxidising must, for example, always be stored in a fireproof cabinet.
3.8 DRY CHEMICALS, LIQUIDS
When not in use, chemicals must be stored in the designated storage location. Chemicals that are stored in the
laboratories must be stored on designated shelves or in designated cupboards. Impermeable packaging must be
used. Liquids/oils must be stored in ventilated cupboards. Chemicals must not be stored on workbenches or in
fume cupboards.
3.9 CHEMICALS STORED IN A FRIDGE/FREEZER
Fridges are primarily to be used for chemicals that are temperature-sensitive. If the fridge does not have
ventilation, the chemicals must be stored in suitable containers/jars. Packaging must, as far as possible, be
impermeable. Round bottomed flasks and Erlenmeyer flasks must if necessary only be used for a limited
period (days) and must be closed. Chemicals with a limited shelf-life must be destroyed at expiration of the
shelf-life, not stored. At least once very six months, fridges and cold rooms must be emptied of chemicals that
are not going to be used in the near future.
3.10 IN-HOUSE MANUFACTURED CHEMICALS
A suitable closed container must be used to store in-house manufactured chemicals. The container must be
labelled with the name of the substance, its structure, register no./production no., date, and the identity of the
person responsible.
3.11 ETHANOL
Ethanol must be stored in a locked cupboard. Stock solutions in small amounts can be stored in the
laboratories.
3.12 ACIDS AND BASES
Acids and bases must be stored in dedicated cupboards that are marked with the correct hazard symbol. Acids
and bases must always be stored separately, and be placed in suitable secondary containment. Stock solutions
in small amounts can be stored in the laboratories
3.13 CORROSIVE SUBSTANCES
Corrosive substances must always be stored as low as possible, and in all circumstances below head height.
This is to reduce the risk of personal injury in the event the container should tip over.
3.14 GASSES
Gas cylinders must always be stored upright, and must be secured with a chain or similar to avoid tipping of
the cylinder. The chain must be affixed above the cylinder’s centre of gravity. Rooms in which pressurised gas
is stored must be marked with the correct hazard symbol. This is to avoid the risk of personal injury, in
particular, in the event of a fire, to fire-fighting personnel.
4
TRAINING
The Head of Department must ensure that all employees are familiar with the requirements and regulations for
storing chemicals, and that necessary instruction is given.
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School of Pharmacy HSE Manual: Common routines
5
DISPOSAL OF CHEMICAL WASTE
5.1 GENERAL
One of the engineers in the subject group Medicinal Chemistry, Department of Pharmaceutical Chemistry, is
responsible for the handling of chemicals, and implementation of the routines described in Chapter 5.
Chemical waste that can be destroyed simply in an environmentally and responsible way in the unit, must not
be handed in but is to be destroyed locally. Destruction in the laboratory: Use the procedures described in
“Kjemiske stoffer [Chemical substances]”, Vita data A/S, 1991, or confer with the advisor or engineer in the
medicinal chemistry group.
Other hazardous chemical waste (that cannot be included under “organic solvent waste” or “pharmaceutical
waste”) must be delivered to the chemicals waste store in the pharmacy building, room 009, and sorted into
the allocated boxes. It is important that chemicals containing heavy metals and reactive chemicals are put into
the correct box. The boxes must be labelled in accordance with the substance categories in the table in section
5.7.
Admission to room 009 is permitted only in company with the engineer in the medicinal chemistry group.
Appointments with the engineer must be arranged in each case.
Every six months, chemical waste will be declared by the person responsible for chemicals disposal and sent
to approved special waste collection centres (see section 5.7: “Other chemical remnants – delivery to waste
collection centres”).
5.2 ORGANIC SOLVENTS WASTE
Organic solvents waste that contains less than 10% water must be emptied into the appropriate remnants
containers for organic solvents waste. (Low water content facilitates incineration).
Rests of organic solvents that contains more than 10 % water must be emptied along with halogenated
solvents into the right tank in the Workshop yard at the Department of Chemistry.
HALOGENATED SOLVENTS
All halogenated solvents must be emptied into dedicated remnants containers found in each laboratory. These
containers must be labelled “Halogenated organic solvent remnants”. Examples of such substances are carbon
tetrachloride, chloroform, methylene chloride, trichlorethylene, chlorbenzene, etc., as well as nitrobenzene.
NON-HALOGENATED SOLVENTS
Remnants of non-halogenated organic solvents must be emptied into the remnants containers located in each
laboratory. These containers must be labelled “Non-halogenated organic solvent remnants”.
VOLATILE ORGANIC SOLVENT REMNANTS (BENZENE, ETHER, ETC.)
Small quantities can be evaporated off in the fume cupboard without application of heat, or outdoors in an
isolated area.
When the organic solvent waste remnant containers are full, they must be emptied into the collection tanks in
the Workshop yard at the Department of Chemistry (see picture). Cans/bottles with non-halogenated solvent
remnants are emptied into the left tank. Cans/bottles with halogenated solvent remnants are emptied into the
right tank. Remember to note what you have emptied in the books in the cupboard by the tanks. Use
protective eye goggles (also to be found in the cupboard). The key to the tanks can be obtained from Central
Stores or the engineer in the Medicinal Chemistry group.
If the collection tank for the non-halogenated waste is full, the waste can be disposed of together with the
halogenated waste. Halogenated waste must NEVER be disposed of into the collection tank for nonhalogenated waste.
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School of Pharmacy HSE Manual: Common routines
The shed with the waste tanks
5.3 PHARMACEUTICAL WASTE
Pharmaceuticals in small quantities may be handed into the chemicals waste stores in the Pharmacy building,
Room 009 (the key can be obtained from the office in Reception). Larger quantities must be packed in hazard
waste boxes and placed in Room 009 in the Pharmacy building.
5.4 COMBUSTIBLE PAPER WASTE
Paper towels and similar that could self-ignite must be rendered safe before discarding. The paper must be
placed in a fume cupboard to evaporate off. Waste containers for flammable waste can be provided (red metal
containers that the users empty).
5.5 GLASS WASTE
Glass waste is collected together and brought to special containers in the Workshop yard. NB! Chemical
bottles must be completely empty and cleaned before disposal. NB! Very important: Only clean glass is to be
put in this container. No ceramics, plastics, metals, paper or fluorescent light bulbs.
5.6 SYRINGE NEEDLES CONTAINING SOLVENT REMNANTS, ETC.
These are placed in special dispenser boxes (can be obtained from the Central Stores at the Faculty of
Chemistry) or empty chemical jars. If the syringe needles do not contain hazardous materials, the boxes can
be screwed tightly and disposed of with normal waste. Otherwise they are disposed of in the Hazard waste
boxes that are then taken to the Pharmacy building, Room 009. They are collected from here by a company
that takes them directly to incineration.
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School of Pharmacy HSE Manual: Common routines
5.7
OTHER CHEMICAL REMNANTS – DELIVERY TO WASTE COLLECTION CENTRES
5.7.1 NB! Explosive, self-igniting, infection-hazard, radioactive substances, or organic peroxides are not
accepted. This type of waste must be handled individually and separately.
5.7.2 Packing and sorting
Sorting must first be undertaken by the person responsible for the Chemicals routine at the School of
Pharmacy as stipulated on the NORSAS forms. The form and guidelines must be ordered from NORSAS AS,
see www.norsas.no. The following are the most-used substance categories with corresponding EAL codes at
the School of Pharmacy:
Substance
no.
7041
7042
7043
7053
7055
7081
7083
7091
7092
7122
7123
7131
7132
7133
7134
7151
7152
7220
7250
7091
7030
Waste category
EAL code
Organic solvents, halogenated
Organic solvents, non-halogenated
Paints, adhesives, varnishes - solvent-based
Paints, adhesives, varnishes - water-based
Pressurised cans
Mercury-containing waste
Cadmium-containing waste
Inorganic salts containing heavy metals
Lead accumulators
Highly reactive substances
Organic peroxides
Acids, inorganic
Bases, inorganic
Cleaning agents
Acidic organic waste
Organic waste, halogenated
Organic waste, non-halogenated
Photographic chemicals
Asbestos insulation
Inorganic chemical remnants
Oil emulsions
140602
140603
080111
080111
160504
060404
060405
060313
160601
160506
160508
200114
200115
200129
160508
160508
160508
200117
170601
160507
120109
PACKING:
The chemicals are packed in boxes. The box must first be equipped with a solid plastic bag. Each box must
only contain chemicals that have the same substance no. The boxes/containers must be tightly closed and
packed vertically. Boxes with plastic bags (Waste containers for hazardous waste), can be obtained from
VWR (low type with bag 50 L, permitted weight 12.5 kg, pack of 10, article no.: 129-0042). The Technical
Department pays for these. Several layers of small bottles can be packed in each box, but a powder must also
be added that not only neutralises but also absorbs water and knocks. Perlite can be used which can be bought
by the sack from Renor. First place a little Perlite in the base of the box, then spread a little between each layer
of small bottles, and finally a little on the top before closing. NB! Do not mix chemicals that can react with
each other and develop poisonous gasses. The worst case scenario is acids and cyanides that could generate
hydrocyanic acid if the contents of the box break.
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DECLARATION:
The form “Declaration of special waste” can be bought from NORSAS.
UiO’s organisation number is 971035854.
Invoicing address: Universitetet i Oslo, Stedkode 32 20 12, Sentralt fakturamottak, pb 1074 Blindern, 0316
Oslo [University of Oslo, Site code 32 20 12, Central Invoicing Office, PO 1074 Blindern, N-0316 Oslo]
The number of kilos/litres is filled in either by the person responsible for Chemical Disposal or the transport
company that collects the waste.
Number of containers: The number with the same substance no. that the Declaration form applies to. Mark the
boxes with the Declaration number to be found on the top right of the form.
Description: Example: “Remnants of strong acids from the Chemistry laboratory” or “Mercury thermometers
and other mercury remnants from laboratories” or similar descriptions.
Transport classification:
Tick the ADR class that applies for road transport.
ADR classes:
Class 1:
Explosive substances and articles
Class 2:
Gasses, compressed, liquefied, or dissolved under pressure
Class 3:
Flammable liquids
Class 4.1: Flammable solids
Class 4.2: Substances liable to spontaneous combustion
Class 4.3: Substances which in contact with water emit flammable gasses
Class 5.1: Oxidising substances
Class 5.2: Organic peroxides
Class 6.1: Toxic substances
Class 6.2: Infectious substances
Class 7:
Radioactive materials
Class 8:
Corrosive substances
Class 9:
Miscellaneous dangerous substances and articles
TRANSPORT TO WASTE COLLECTION STATION
Call Ragn Sells, tel. 08899, or mail to [email protected]
6
REVIEW
This routine must be reviewed as required.
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School of Pharmacy HSE Manual: Common routines
CORRECT USE OF FUME CUPBOARDS
PURPOSE
To establish general basic rules for all work in fume cupboards at the School of Pharmacy. The rules must
contribute to high levels of safety in the laboratory. Everybody must comply with the same basic rules and
have the same expectations with regard to how to work in and use fume cupboards so that accidents and injury
are prevented.
RESPONSIBILITY
The immediate line manager in each laboratory is responsible for ensuring that the rules for working in fume
cupboards are followed. The line manager is also responsible for pointing out and following up any breach of
the rules.
Each person who works in one of the School’s laboratories is responsible for familiarising themselves with the
rules for working in fume cupboards and for complying with them.
The Head of Department/line manager is responsible for ensuring that any necessary protective gear is
available.
PROCEDURE
STANDARD WORK ROUTINES FOR WORKING IN FUME CUPBOARDS

Personal protective gear must be used as required.

Ensure that the fume cupboard is cleared of unnecessary equipment, flasks, etc. before starting work.
Flasks and equipment impair the laminar airflow in the cupboard and diminish the extracting efficacy.

Check the safety height of the sash. Normally, this is 25-30 cm. When working, this height must not
be exceeded. If however this height is exceeded, the volume of air from the cupboard must be
increased above normal. Some cupboards have automatic regulation of the air volume (constant flow).

Choose your working position (sitting or standing) based on the risk of liquid splash and the duration
of the work. Never bend your head in the cupboard, but re-assess the work method or the test set-up.

Position the work (volatile liquids, materials that release dust, etc.) as far as possible in the centre of
the cupboard. Generally, suction is strongest here. Work using calm movements in the cupboard. This
stops turbulence and movement of polluted air into the breathing zone.
TIDINESS AND CLEANLINESS IN FUME CUPBOARDS

Tidy and clean after working. The fume cupboard must not be used for storage. Inflammable,
corrosive, irritating liquids, chemicals, and similar must be put back in the chemicals cupboard with
ventilation.
CONDUCT WHEN HANDLING CHEMICALS

Do not expose others or yourself to unnecessary health hazards. Use the fume cupboards. Transport of
chemicals between the chemicals cupboard and the fume cupboards must take place with minimum
risk to yourself and others in the laboratory.

At tests that involve the use of hazardous chemicals, a copy of the HSE datasheet must be easily
accessible in the laboratory.

At tests that involve the use of hazardous chemicals, the work procedure must be designed to take this
into account, so that necessary technical checks of process equipment and safety equipment are
conducted in advance. It must be ensured and documented that the equipment and materials are
appropriate, and that necessary safety equipment is available and functioning.

The quantity of chemicals in the fume cupboard must be limited to that necessary for the work in
progress.
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School of Pharmacy HSE Manual: Common routines
PERSONAL PROTECTIVE EQUIPMENT
Personal protective equipment must be available in the laboratory and must be used if conditions dictate this:
i.e. eye protection, gloves, and laboratory coat. Face protection is shared between the laboratories as required.
Other personal protective equipment (e.g. breathing apparatus) can be obtained as required. Use a fume
cupboard if the work requires this. Contact lenses can be worn if they are used in combination with eye
protection.
FIRST AID EQUIPMENT
First aid equipment must be easily accessible in each laboratory.
1. An emergency shower must be installed just outside every laboratory.
2. Eye douches: Research laboratories: Minimum one per person.
(Course laboratories: Minimum one per 5 persons)
3. First aid cupboard/box must as a minimum contain:
Plasters, scissors, all-in-one packs, bandages, 1 bottle chlorhexidine.
4. Fire blankets: Minimum 1 fire blanket in each laboratory.
LABORATORY TESTS
Laboratory tests must undergo risk assessment and be recorded.
Risk assessment: A systematic assessment to find out which undesired events could occur, what could be done
to prevent these, and what is necessary to minimise the impact if something should nevertheless happen.
TIDINESS
Everybody is responsible for keeping their laboratory tidy and for clearing up after themselves in other rooms.
The laboratories must be kept clean and tidy at all times. Contact the person responsible for the room if
necessary.
The laboratories must be locked (and windows closed), when they are vacated to prevent access by
unauthorised persons.
Normal laboratory working hours are from 8:00 - 16:00.
The name of the person responsible for the room must be posted at the entrance to all special rooms. The
person responsible for the room has a particular responsibility for following up the activities in the room and
for ensuring that users tidy up after themselves. It is not their responsibility to tidy up after others.
Chemicals/equipment must not be lent out/removed from the place of use without the permission of the person
responsible for the room or for the instruments. The person responsible for the room or for the instruments
must be notified of any defective equipment.
Each laboratory must have the School’s HSE Manual (relevant sections) and folder with HSE datasheets
visible and easily accessible; chemicals cupboards with ventilation (as required) for storage of dangerous
chemicals; and fume cupboards (as required) for working with these chemicals.
Breach of HSE regulations (the UiO HSE Manual; the Faculty of Mathematics and Natural Sciences HSE
Manual; the School of Pharmacy HSE Manual; and the Working Environment Handbook for Students) must
be notified to the person responsible for reporting/remedy.
All orders from the Safety Delegate must be complied with.
UNAUTHORISED PERSONS
Notify the Security Centre if unknown persons are discovered in the laboratory or on other premises to which
they do not have access. From and including 01.10.2002, employees are permitted to restrain/request proof of
identity.
NB! Minors are not permitted in the laboratories.
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School of Pharmacy HSE Manual: Common routines
SAFETY DATASHEETS AND THE SUBSTANCES AND CHEMICALS REGISTER
Safety datasheets must be read prior to opening any chemicals container. Be aware that the contents of a new
chemical container of a specific product can be more reactive than the contents of an old one.
The School of Pharmacy will via a person responsible for the Substances and Chemicals Register in each
department, maintain the School’s collection of electronic safety datasheets and localisation of the School’s
chemicals (ECOonline Substances and Chemicals Register). The user must inform the person responsible for
the Substances and Chemicals Register of any changes in the chemicals stock.
The users/groups must maintain a separate or joint binder with material safety data sheets in hard copy on site.
If the updated safety data sheets are missing in their own binder, a new version should be printed from the
School’s electronic Substances and Chemicals Register (ECOonline).
BIOLOGICAL MATERIALS AND CULTURES
When working with biological materials or cultures it is important to wash your hands frequently in order to
avoid infection. Use hand cream as well. Solutions that contain live materials, virus, or plasmids must be
autoclaved prior to disposing of the solution down the sink. Bottles and flasks with contents must be labelled.
The label must correspond to the content. Remove the label before the equipment is sent for washing. Do not
use other people’s pipettes without asking!
CHEMICALS
Chemicals must be treated with caution because most of them are poisonous and several of them react with
air, water, alcohols, etc. Read the safety datasheet prior to opening the chemical container and prior to use.
(The date for receipt of the chemical container from the supplier is to be noted, if not already noted, on the
container).
Substantial changes in the chemical stocks must be notified to the person responsible for the Substances and
Chemicals Register.
Dangerous chemicals that are not in use must be stored in the chemicals cupboard with ventilation. This also
applies to remnant cans containing organic solvent remnants. The only exception is concentrated hydrochloric
acid (HCl) which can be placed in the fume cupboard to prevent corrosion of the chemicals cupboards.
All chemical containers must be marked and a list of codes (that explain the contents) must be easily
accessible.
Chemical spills must be removed immediately. Remnant cans must not be so full that they overflow. The
scales must be clean.
Laboratory chemicals must not be stored or used in premises designed for other use (e.g. offices). Fume
cupboards must be used when working with dangerous chemicals.
See in addition Routines for handling and storing chemicals at the School of Pharmacy [Rutine for håndtering
og lagring av kjemikalier ved Farmasøytisk institutt] under Common routines.
PHARMACEUTICALS
Pharmaceuticals and medicine formulations are handled in accordance with the safety datasheets for the active
ingredients. Narcotic substances (Group A) must be stored in a safe.
GAS CYLINDERS
Gas cylinders must be securely affixed so that they cannot tip over. The outside of the laboratory doors must
be marked with a warning sign for gas cylinders. Transport of gas cylinders must only take place after the gas
regulator has been removed from the cylinder. The gas cylinder is highly pressurised (normal max. pressure is
about 200 bar), which is enough to send the cylinder through the wall like a rocket if the gas cylinder falls and
the gas regulator is knocked off.
GAS REGULATOR
NB! When mounting the gas regulator, the threads must not be lubricated with grease, as oxygen in particular
can ignite the grease! Be sure that all valves are switched off/closed prior to opening the cylinder.
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School of Pharmacy HSE Manual: Common routines
Valves: Gas cylinder main valve = 1, Gas regulator main valve = 2.
Closing the gas cylinder: Turn (1) clockwise (inwards), turn (2) counter-clockwise (outwards).
Opening the gas cylinder: Turn (1) counter-clockwise (outwards), turn (2) clockwise (inwards).
ACETYLENE
If “strange” noises can be heard from a gas cylinder containing acetylene, a chain reaction could be occurring
inside the cylinder that can increase the pressure such that the cylinder bursts. Acetylene is an extremely
flammable gas.
Evacuate the building. Contact the Fire services.
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School of Pharmacy HSE Manual: Common routines
INSTRUMENTS AND HAZARDS
DEWAR VESSELS
Danger of implosion due to internal vacuum. Cold solutions must not be poured on the edge of the vessel!
HPLC (HIGH PRESSURE LIQUID CHROMATOGRAPHY)
Overpressure can burst tubes resulting in solvent/sample splash.
FRIDGES (STANDARD)
Must not be used to store flammable chemicals due to the fact that sparks from the thermostat and light bulb
present a fire and explosion hazard.
COMPRESSOR AND FLUID BED APPARATUS
Take care to de-pressurise tubes before dismantling.
LABORATORY DISHWASHERS
Keep the area around the machine tidy. Remove clean glass equipment when the machine has finished.
Examine and clean base and base filter. Broken glass must be disposed of in the waste glass container.
NMR
NMR instruments are surrounded by a magnetic field which can damage cards with magnetic strips (e.g. mini
bank cards, key cards, borrowing cards), laptops (strips data from the hard disc), clocks, electronic equipment.
People with pacemakers and those who have implants/implanted parts of magnetic metal in the body are not
allowed to enter the NMR lab. This is for their own safety since the pacemaker may stop functioning.
ROTAVAPOR
Read the entire user instructions prior to using the rotavapor.
Follow the safety instructions in the SOP.
The rotavapor must only be used by employees and master students who either through training or own
professional experience have a sound understanding of the dangers that can arise at use of the equipment.
Those who have not received training must not use the rotavapor by themselves.
Round bottomed flasks with cracks/hairline cracks must not be used as they can implode (explode inwards)
due to the vacuum. Rotavapors should preferably be connected to membrane pumps with cold traps and
exhaust to the fume cupboard, and should not be connected to a water jet pump where much of the solvents
that evaporate will disappear down the drain.
Personal protective equipment such as eye protection and lab coats must always be used when working with a
rotavapor.
Hard copies of the user instructions and the SOP (standard operating procedure) for the type of rotavapor must
always be available next to the equipment.
Refer to the School of Pharmacy common area N:\HMS for the following user instructions for use of the
rotavapor: Büchi Rotavapor Operation Manual.pdf.
This contains a good description of correct use, hazards, and general safety at use of a rotavapor.
ULTRASOUND BATH
Shakes molecules loose. Do not put body parts into the vessel.
UV LAMPS (HAND-HELD LAMPS WITH AND WITHOUT STAND AND IN INSTRUMENTS)
Short wavelength light (254nm) and long wavelength light (365/366nm). UV light must never be directed at
people. The wavelength and energy are inversely proportional. This means that the shorter the wavelength the
greater the energy and the greater the danger.
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School of Pharmacy HSE Manual: Common routines
HOT AIR PISTOL (ELECTRIC)
High temperature/strong heat. Exercise caution when using. Turn off when not in use. Hot air pistols can
cleave products, ignite solvents, and cause fires and burns.
SCALES
Must be kept clean to avoid exposure to chemicals or biological materials.
TOXIC SUBSTANCES AND HAZARDS
All users are responsible for checking the safety datasheets prior to using the chemical/substance. See the
School’s Substances and Chemicals Register that describes the hazards and preventive measures:
www.ecoonline.no
DECONTAMINATION OF BIOLOGICAL WASTE AND EQUIPMENT
MAIN WASTE GROUPS
A. Disposable equipment such as pipettes, pipette tips, Eppendorf tubes, syringes and used microscope slides.
B. Solid biological waste, e.g. used agar dishes and cell cultivation equipment.
C. Suspensions of cells, bacteria or virus and the equipment these have been in contact with,
e.g. cultivation flasks, glass pipettes, test tubes and centrifuge tubes with lids.
DISPOSAL OF WASTE - TYPES A AND B
Such waste is to be placed in specially marked cartons for hazardous waste.
The cartoons must be lined with correspondingly marked waterproof yellow/black plastic sacks.
NB! Do not overfill the cartons as they must be closed without compressing the contents.
When the boxes are full, they are to be brought down to the waste disposal room next to the lift in the 2nd
lower floor. Ragn Sells will take them from here to be incinerated.
DISPOSAL OF WASTE - TYPE C
There are two ways of treating the solution before it is disposed of down the sink.
Hazard class I:
1. Autoclave
2. Chemical destruction Addition of sodium hydroxide pearls, leave for 30 mins.
Example
MAXI PREP.
Culture remnants:
autoclaved
Supernatant:
Tipped back into the culture flask and autoclaved
Centrifuge tubes: Inactivated with alcohol
MINI PREP. SMALL VOLUME IN SEALED TUBE IN HAZARDOUS WASTE
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School of Pharmacy HSE Manual: Common routines
Hazard class II-2 and sporogenous bacteria:
Mandatory autoclaving
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School of Pharmacy HSE Manual: Common routines
AUTOCLAVING PROCEDURES
Bacteria and cell cultures that are to be autoclaved are placed in the washroom in metal trays (marked area).
- Test tubes are placed in separate, suitable racks in empty autoclaving trays.
- Centrifuge tubes are filled with water and put in the same place.
- To avoid deformation, the centrifuge tubes must be open. Only centrifuge tubes in PP plastic can be
autoclaved.
- Soft-agar tubes and corex tubes can be placed in autoclaving trays.
INACTIVATION PROCEDURES
METHODS FOR CHEMICAL DISINFECTION
1. SODIUM HYDROXIDE PEARLS
- Inactivation of cultures, e.g.: E. coli 2 pearls/100ml
2. 5% CHLORAMINE
- Disinfection at spills on benches.
3. VIRKON
- Disinfection of equipment that cannot be autoclaved.
- Removal of any spills on benches.
4. DECONEX
- Primarily for difficult cleaning, but has disinfectant properties.
5. RECTIFIED ALCOHOL - 70%
- Disinfection of equipment that cannot be autoclaved.
- Removal of any spills on benches.
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School of Pharmacy HSE Manual: Common routines
WASTE HANDLING
The user is responsible for ensuring that all waste they generate is destroyed and/or taken to the nearest
collection point. See in addition Routines for handling and storing chemicals at the School of Pharmacy
[Rutine for håndtering og lagring av kjemikalier ved Farmasøytisk institutt] under Common routines.
NORMAL WASTE/REMNANT WASTE
Only non-hazardous waste is to be handled without gloves: Plastic waste, food waste, dirty paper, and similar.
Normal waste must not contain sharp objects, etc. that could injure cleaning staff.
Larger quantities of plastic and/or polystyrene are to be taken to the outside waste remnants containers by the
user.
The remaining normal waste is collected by the cleaning staff.
PAPER WASTE
Paper and cardboard. Paper waste must be disposed of in the paper waste container by the user.
Containers for waste paper are located at:
- Pharmacy building platforms in the main stairs on each floor every Thursday at 09-13
- Physics building by the east lift on each floor on Fridays at 09-13
- Chemistry building by the east lift on each floor on Thursdays at 09-13
- ZEB building by the lift on the 3rd floor every Thursday at 09-13.
GLASS WASTE
Cleaned glass waste, broken glass and TLC plates.
(The following are not glass waste: Used silica, chemical remnants, plastic corks and fluorescent light bulbs.
Used silica must be handed in as chemical waste, and contaminated glass must be packed in well and disposed
of in the hazardous waste boxes.)
Glass waste must be taken by the user to the glass waste containers located at:
- Pharmacy building, Room 025 and the workshop yard in the Chemistry building.
- ZEB building. Glass waste must be put in glass waste boxes and placed in the “waste room” located
by the side of the lift in ZEB 2U.
HAZARDOUS WASTE
Waste that cannot be disposed of safely together with consumer waste because it could cause serious pollution
or risk of harm to people, animals, birds and fish. Chemical waste is described on page 20.
EE WASTE (ELECTRIC AND ELECTRONIC WASTE)
Waste such as white goods, cables and leads, computer equipment, office machines, measuring instruments,
medical equipment, circuit cards, monitors, etc. must be disposed of in an EE waste container (must be
ordered) after agreement with the local person responsible for equipment. The person responsible for IT must
be notified when disposing of computer equipment.
HAZARDOUS WASTE
Biological waste, contaminated single-use items, and syringe needles must be packed in “hazardous waste
boxes” (cardboard boxes with associated thick plastic bag). Sharp objects must be packed well before putting
in the plastic bag. Syringe needles/scalpels can for example be packed in sealed plastic boxes. Infected
materials of all types, small quantities of solvents in sealed containers, and gels with toxic substances can be
disposed of. When the boxes are full, they must be properly closed, labelled, and taken to the Pharmacy
building, Room 009.
In the ZEB building, the hazardous waste boxes are taken to the waste room next to the lift in ZEB 2U. The
hazardous waste boxes will then be sent to be incinerated. NB! Organic solvents should not be put in the
hazardous waste boxes as they can cause uncontrolled incineration.
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School of Pharmacy HSE Manual: Common routines
WASTE ARISING FROM WORK WITH CELLS CLASSIFIED AS BSL-2
(BIOSAFETY LEVEL 2)
BSL-2 work in cell laboratories involves heightened safety routines compared to BSL-1(normal cell work).
The most important requirements relate to signage of areas with restricted access when work is ongoing, and
at waste treatment (autoclaving).
BSL-2 applies to pathogens (e.g. virus) that are associated with disease in humans. Preventive or therapeutic
measures are often available.
Work routines (standard microbiological practice) for BSL-2:
Access to the laboratory is restricted when work with virus/virus-infect cells is ongoing. The door into the
laboratory and the LAF bench (biosafety cabinet) must be marked with the BSL-2 sign, the name of the study,
and the duration of the study.
Personnel must have received training in working with viruses prior to granting authorisation (a contract is
drawn up that is signed, contact the person responsible).
Work in the cell lab is performed in an LAF bench that is approved for such work.
Liquid waste such as medium, solutions containing virus, etc. is disposed of into flasks containing Virkon
solution that are situated in the LAF bench. For suction, the Virkon solution is added to the collection
chamber; liquid waste is autoclaved and can then be disposed of down the sink.
Pipettes, pipette tips, etc. are placed in a dedicated waste bucket (see below). Pipettes must be rinsed in Virkon
solution before they are put in the waste bucket.
Mark incubators with the virus sign during incubation.
Work with the cells in accordance with the BSL-2 standard until they have been lysed with sodium hydroxide
or other (SDS solution, chloroform-methanol). Frozen cells in water should be treated as BSL-2.
Disinfection (decontamination):
Virus waste is disinfected with Virkon solution. Follow the instructions for use (www.puls-norge.no).
Suction and tubes must be rinsed with Virkon solution and then with 70% ethanol.
The LAF bench must be first washed with 2% SDS solution and then with 70% ethanol.
Leave the LAF bench under UV light overnight.
BSL-2 waste:
Waste (pipettes, cell flasks, medium) must be put in red bags marked hazardous waste. The bags are placed in
stainless steel metal containers that must be used only for this type of waste.
All waste must be autoclaved.
After autoclaving, the waste can be disposed of as hazardous waste in yellow boxes. When the boxes are full,
they must be closed properly, labelled, and taken to the Pharmacy building, Room 009.
33
School of Pharmacy HSE Manual: Common routines
RADIOACTIVE WASTE
All waste that has been in contact with radioactivity must be put in special waste boxes. There must be one
box for C14 and one for H3 waste. Non-radioactive (hazardous) waste must not be disposed of together
with radioactive waste. Waste from work with P32 must be disposed of in plexiglass containers.
For collection of the waste, contact the local (Arild Rustan for FI, Ewa Jaroszewicz for ZEB) or central
Radiation Safety Coordinator (Elin Agathe Hult) at UiO.
34
School of Pharmacy HSE Manual: Administration
ROUTINES AT THE ADMINISTRATION
HSE
Health, Safety and the Environment in the
workplace
Version 2010
Universitety of Oslo
School of Pharmacy
Administration
35
School of Pharmacy HSE Manual: Administration
TABLE OF CONTENTS
In general .................................................................................................................................................... 36
Reception .................................................................................................................................................... 36
Staff issues .................................................................................................................................................. 36
Courses – examination ................................................................................................................................. 36
Curriculum .................................................................................................................................................. 36
IN GENERAL
This is an overview of the routines in the Administration that are relevant as for the HSE work.You will find
an overall presentation of the routines in the Administration at N:\Administrasjonens_Rutinehandbok_kopi.
RECEPTION
1.2
1.3
1.9
1.13
1.15
1.16
1.20
Admission card
Keys
Dealing with pure alcohol
Preparing the teaching rooms
Students’ events
Fire instructions at events in the room BL25 054/055
Contact with the Technical Department
STAFF ISSUES
3.3
3.4
3.6
3.7
3.8
3.10
Registering new employees
Absence registration
Self-certified sick leave/doctor’s certificate
The handling of issues involving staff and teaching problems
Quitting approvement
Reporting deaths
COURSES – EXAMINATION
6.7
6.11
Fire alarm during examination
Complaint about the censorship
CURRICULUM
7.4
36
Evaluation of subjects
School of Pharmacy HSE Manual: Department of Pharmacy
ROUTINES AT THE DEPARTMENT OF PHARMACY
HSE
Health, Safety and the Environment in the
workplace
Version 2010
Universitety of Oslo
School of Pharmacy
Department of Pharmacy
37
School of Pharmacy HSE Manual: Department of Pharmacy
TABLE OF CONTENTS
Installations regarding health, security and environment............................................................................... 39
BL25010B Tablet room ............................................................................................................................... 39
BL25011 ..................................................................................................................................................... 40
BL25012 ..................................................................................................................................................... 41
BL25029 ..................................................................................................................................................... 42
BL25167 BL 25168 ..................................................................................................................................... 43
BL25176 Students’ lab courses’ laboratory .................................................................................................. 44
BL25179 Bl 25179C .................................................................................................................................... 45
BL25183 ..................................................................................................................................................... 46
BL25186 ..................................................................................................................................................... 47
BL25188 ..................................................................................................................................................... 48
BL25224 ..................................................................................................................................................... 49
38
School of Pharmacy HSE Manual: Department of Pharmacy
INSTALLATIONS REGARDING HEALTH, SECURITY AND ENVIRONMENT




Emergency shower in the womens lavatory in the ground floor (room 172)
Eye washer
Fire blanket
First aid kits in room 010B – 029 – 167 – 176 – 224
BL25010B TABLET ROOM
Room responsible person:
Training responsible person:
Helge Gundersen
Helge Gundersen
ROOM ACTIVITIES


Rooms containing larger pharmaceutical processing equipment.
Tablets – pellets – spraydrying
RULES





It is not allowed to use the instruments after normal working hours.
Tablet producing machines should not be unattended while in use .
Use a hood and a labcoat
Face shield, protective goggles and ear protection. Rests from the production is classified as
hazardous waste.
Evacuate the air from the units under pressure before disconnection.
Risk
High-power processing equipment,pressureized air,
dust, noise
Preventive measure
Coat, hood, ear protection, face shield
39
School of Pharmacy HSE Manual: Department of Pharmacy
BL25011
Room responsible person:
Training responsible person:
Helge Gundersen
Helge Gundersen
ROOM ACTIVITIES
Fluid.- bed for coating/granulating.

RULES


Protective goggles
Use ear protection, very noisy room.
Risk
Pressureized air, dust, noise
40
Preventive measure
Ear protection, face shield
School of Pharmacy HSE Manual: Department of Pharmacy
BL25012
Room responsible person:
Training responsible person:
Helge Gundersen
Helge Gundersen
ROOM ACTIVITIES

Temperature controlled cupboard and air compressor.
RULES


Protective goggles
Use ear protection, very noisy room.
Risk
Pressureized air, dust, noise
Preventive measure
Ear protection, face shield
41
School of Pharmacy HSE Manual: Department of Pharmacy
BL25029
Room responsible person:
Training responsible person:
Hoai T. N. Aas
Hoai T. N. Aas
ROOM ACTIVITIES

Laboratory for sterile production.
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Autoclave
Hoai T. N. Aas
Risk
42
Training responsible
person
Hoai T. N. Aas
Preventive measure
Comments
May be used during the naight
School of Pharmacy HSE Manual: Department of Pharmacy
BL25167 BL 25168
Room responsible person:
Training responsible person:
Helge Gundersen
Helge Gundersen
ROOM ACTIVITIES


Laboratory
Lab course preparation room
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Cenrtifuge IEC Centra MP4
Freezedryer
Other instruments
Tove Larsen
Tove Larsen
Helge Gundersen
Training responsible
person
Tove Larsen
Tove Larsen
Helge Gundersen
Comments
Can be used during the night
RULES
The water supply for the the lab washer and the water still must be turned out
during the night.
Risk
Strong acids
Organic solvents
Labwasher and water still
Preventive measure
Protective goggles, work in a fume hood
Store in a chemical locker, and work in a fume hood
Turn out the water supply at the end of the day..
43
School of Pharmacy HSE Manual: Department of Pharmacy
BL25176 STUDENTS’ LAB COURSES’ LABORATORY
Room responsible person:
Tove Larsen
ROOM ACTIVITIES

Students’ lab courses’ laboratory in medicinal formulation
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Freezedryer
Tove Larsen
Risk
44
Training responsible
person
Tove Larsen
Preventive measure
Comments
Can be used in the night
School of Pharmacy HSE Manual: Department of Pharmacy
BL25179 BL 25179C
Room responsible person:
Training responsible person:
Tove Larsen
Tove larsen
ROOM ACTIVITIES

Production of and characterisation of nano particles
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
All instruments
ToveLarsen
Training responsible
person
Tove Larsen
Comments
SOP for using the equipment can be found in a binder in the bookshelf in room 180b
Reservations for using the equipment should be noted on the whiteboard.
RULES




All dishes should be rinsed with water or alcohol, and then be collected in the blue tub to be washed
in the lab washer, or kept soaking in the blue tub. Water should be replaced when needed, baut at
least every week.
Keep things tidy, refill commodities when needed..
All stored samples should be labelled.
Rotavapor and extruder should not be used after normal working hours.
Risk
Rotovapor and extruder (vacuum and pressure),
PRESSURIZED GAS
Preventive measure
SOP and attendance
See section “The laboratory: Safety and the working
environment” in this handbook, to ensure correct
handling of pressurized gas cylinders
45
School of Pharmacy HSE Manual: Department of Pharmacy
BL25183
Room responsible person:
Training responsible person:
Hoai T. N. Aas
H. Aas- H.H. Tønnesen- T. Larsen
ROOM ACTIVITIES

Instruments’ laboratory with gas
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Suntest, chromometer
DSC
HPLC
H.H. Tønnesen
T. Larsen
H. Aas
Risk
Rotovapor and extruder (vacuum and pressure),
PRESSURIZED GAS
46
Training responsible
person
H.H. Tønnesen
T. Larsen
H. Aas
Comments
Preventive measure
SOP and attendance
See section “The laboratory: Safety and the working
environment” in this handbook, to ensure correct
handling of pressurized gas cylinders
School of Pharmacy HSE Manual: Department of Pharmacy
BL25186
Room responsible person:
Training responsible person:
Hoai T. N. Aas
ROOM ACTIVITIES:

Wet laboratory and monochromator room
RULES






Keep chemicals and solvents in the locker.
Solvents should be disposed of in the containers in the fume hood in room 160. Use the dedicated
buckets for transporting solvent bottles.
How to clean the glassware: Put it in a tub with diluted Deconex solution (compounds insoluble in
water are rinsed with alcohol). Wash the glassware in the lab washer. After drying the glassware
should be sorted and placed in their respective drawers and cupboards. (Glassware should not be left
on the drying rack when it’s dry).
Do not leave the lab without refilling commodities.
Always alert the buyer in due time when commodities have to be ordred
The water supply for the water still must be turned out during the night
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
All instruments
Solveig Kristensen
Risk
Rotovapor
Acids and solvents
Pressureized gas cylinders
Training responsible
person
Comments
Preventive measure
Work in a fume hood and always wear protective
gear.
See section “The laboratory: Safety and the working
environment” in this handbook, to ensure correct
handling of pressurized gas cylinders.
47
School of Pharmacy HSE Manual: Department of Pharmacy
BL25188
Room responsible person:
Training responsible person:
Hoai T. N. Aas
H. Aas- H.H. Tønnesen
ROOM ACTIVITIES
 Instruments’ laboratory with gas
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
UV and HPLC
H. Aas
Training responsible
person
H. Aas
Fluorometer
Lifetime spectrophotometer
S. Kristensen
H.H. Tønnesen
S. Kristensen
H.H. Tønnesen
Risk
Pressureized gas
48
Comments
Preventive measure
See section “The laboratory: Safety and the working
environment” in this handbook, to ensure correct
handling of pressurized gas cylinders
School of Pharmacy HSE Manual: Department of Pharmacy
BL25224
Room responsible person:
Training responsible person:
Hoai T. N. Aas
Jan Karlsen
ROOM ACTIVITIES
 Instruments’ laboratory with gas
Risk
Pressureized gas
Preventive measure
See section “The laboratory: Safety and the working
environment” in this handbook, to ensure correct
handling of pressurized gas cylinders
49
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
ROUTINES AT THE DEPARTMENT OF PHARMACEUTICAL
BIOSCIENCES
HSE
Health, Safety and the Environment in the
workplace
Version 2010
Universitety of Oslo
School of Pharmacy
Department of Pharmaceutical Biosciences
50
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
TABLE OF CONTENTS
BL21: Cleaning routines in the ZEB-building ..............................................................................................53
BL21 2U27: Freezer room ...........................................................................................................................54
BL21 2U53: Isotope room, class B...............................................................................................................55
BL21 2U54: Freezing room – 80 ° centigrade ..............................................................................................56
BL21 2U63A: Storage room ........................................................................................................................57
BL21 2U63B: Atomic force microscopy room .............................................................................................58
BL21 2U67: Freezer/cold room ....................................................................................................................59
BL21 2U68: Molecular dynamics storm 860 ................................................................................................60
BL21 1U51: Course room ............................................................................................................................61
BL21 1U53: Centrifuge/bacteria growing room............................................................................................62
BL21 1U53: Cooling room ..........................................................................................................................63
BL21 1U62: Lab course preparation room....................................................................................................64
BL21 1U64: Polaroid camera room (not named) by the elevator ..................................................................65
BL21 1U65: Weighing room ........................................................................................................................66
BL21 1U67: Medium cultivation room ........................................................................................................67
BL21 1U69: Autoclave/cleaning room .........................................................................................................68
BL21 151: Cell cultivation laboratory ..........................................................................................................69
BL21 152: Cold room ..................................................................................................................................70
BL21 153: Microscopy room .......................................................................................................................71
BL21 155: Research laboratory ....................................................................................................................72
BL21 156: Centrifuge room .........................................................................................................................73
BL21 163A: Film developing room..............................................................................................................74
BL21 163B: Electrophoresis laboratory........................................................................................................75
BL21 164: Protein laboratory .......................................................................................................................76
BL21 166: Research laboratory ....................................................................................................................77
BL21 167: Research laboratory ....................................................................................................................78
BL21 168 Instrument room ..........................................................................................................................79
BL21 169: Electrophoresis room ..................................................................................................................80
BL21 170: P-2 room ....................................................................................................................................81
Cleaning procedures in the third floor ..........................................................................................................82
BL25 310: Nitrogen tank in cold storage ......................................................................................................83
BL25 407: Isotope laboratory 1 ....................................................................................................................84
BL25 408: Extraction/thin layer room ..........................................................................................................85
BL25 409: Chemical/weight room ...............................................................................................................86
BL25 411: HPLC room................................................................................................................................87
BL25 413E: Western electrophoresis lab ......................................................................................................88
BL25 413J (outside): Sorval Ultra Centrifuge ..............................................................................................89
BL25 477: DNA/RNA lab ...........................................................................................................................90
BL25 480: Isotopic laboratory......................................................................................................................91
51
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL25 481: Cell laboratory ........................................................................................................................... 92
BL25 484: Microplate reader and protein chromography .............................................................................. 93
52
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21: CLEANING ROUTINES IN THE ZEB-BUILDING
Responsible person: Mona Gaarder tlf.44928/45
RULES
-
Bring the wash down each morning or when the baskets are filled enough
Do the washing in the lab washer.
Put the wash in the electric drying cabinet
After drying distribute to the cabinets, autoclavation and dry sterilization
Bring down floating bacterial waste from all groups for autoclavation
Pack the equipment for autoclavation and place it by the autoclave. Run autoclavation when there is
enough to fill up an autoclave.
- ”Pack” equipment for dry sterilization and put it in the heating oven that is used for drysterilization.
Start sterilization when there is a reasonable amount of equipment.
- Put yellow and blue tips, Eppendorf tubes and kits for autoclavation.
- If you have any spare time, please bring filled risk waste containers down, and clear away glassware
and clutter that has been forgotten. Perhaps you can clean up around for instance the electrophoresis
equipment or other things.
One of the engineers will accompany everyone the first week as a monitor, to ensure that everyone are trained
in using the autoclave, the dry sterilization unit and the lab washers.
53
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 2U27: FREEZER ROOM
(Last update 21.06.2010)
Room responsible person:
Training responsible person:
Ewa Jaroszewicz
Ewa Jaroszewicz/Anne Lise Rishovd
ROOM ACTIVITIES


Storage of bacteria collection
Storage og reagents
RULES

General laboratory rules
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Freezer - 20⁰C
Ewa Jaroszewicz
Training responsible
person
Ewa Jaroszewicz
Freezer - 80⁰C
Ewa Jaroszewicz
Ewa Jaroszewicz
Freezer - 80⁰C
Anne Lise Rishovd
Anne Lise Rishovd
Notes
RISK ANALYSIS
Risk
Storage of spore-forming bacteria.
Precaution
Use safety equipment
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

54
No
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 2U53: ISOTOPE ROOM, CLASS B
(Last update 01.11.2010)
Room responsible person:
Training responsible person:
Ewa Jaroszewicz
Ewa Jaroszewicz, local radiation protection coordinator
for ZEB
ROOM ACTIVITIES

Work with isotop 125I, 32P, 35S
RULES

General rules for isotoplaboratory class B
INSTRUMENTS AND EQUIPMENT
Name/type
Beta/Gamma counter,
scintallation counter
Geiger-Muller detector
Vertical electroforese
Responsible person
Training responsible
person
Ewa jaroszewicz
Ewa Jaroszewicz
Ewa Jaroszewicz
Ewa Jaroszewicz
Ewa Jaroszewicz
Research group
Notes
RISK ANALYSIS
Risk
Exposure to radioactive materials.
Precaution
All users must have completed an approved course.
Read Safety Data Sheet and radiation protection
regulations in advance.
All users have to wear personaldosimeter.
Use safety equipment when handling radioactive
materials.
Use 1 cm perspex (Plexiglass) as shilding.
Work in fume hood.
Regular monitoring for contamination is required.
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY



Eye wash bottles
Plexiglass shield
Plexiglass boxes for radioactive waste
55
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 2U54: FREEZING ROOM – 80 ° CENTIGRADE
Room responsible person: Ragnhild Paulsen (RP), office no. 253, tlf 44936
ROOM ACTIVITIES
Sample storage at minus 80 degrees centigrade in two separate freezers.
RULES
- The door into the room must always be locked.
- Place the samples in labelled boxes, which again are stored in racks. Each rack should be named. Be
careful not to damage the polystyrene covers.
- The freezers’ alarms are (for the time being) not connected to the alarm central. We have to be alerted
if the alarmis activated.
INSTRUMENTER
2 Forma Scientific – 86C Freezer (ultrafreezers).
Responsible person: Ragnhild Paulsen
Training responsible person: Ragnhild Paulsen
56
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 2U63A: STORAGE ROOM
(Last update 21.06.2010)
Room responsible person:
Training responsible person:
Ewa Jaroszewicz
Ewa Jaroszewicz
ROOM ACTIVITIES

Storage of various equipment
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Training responsible
person
Notes
RISK ANALYSIS
Risk
No risk
Precaution
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

No
57
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 2U63B: ATOMIC FORCE MICROSCOPY ROOM
(Last update 22.06.2010)
Room responsible person:
Training responsible person:
Ewa Jaroszewicz
Ida K. Hegna/Ewa Jaroszewicz
ROOM ACTIVITIES


Research
Use of instrument in nanotechnology
RULES

General laboratory rules
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Training responsible
person
Atomic Force Microscop,
Nano Wizard from JPK with
microskop from Nicon
Active vibration isolation
system MOD-1,
HALEYONICS
Ida K. Hegna/
Ewa Jaroszewicz
Ida K. Hegna/
Ewa Jaroszewicz
Ida K. Hegna/
Ewa Jaroszewicz
Ida K. Hegna/
Ewa Jaroszewicz
Notes
RISK ANALYSIS
Risk
No
Precaution
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

58
No
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 2U67: FREEZER/COLD ROOM
(Last update 21.06.2010)
Room responsible person:
Training responsible person:
Ewa Jaroszewicz
Ewa Jaroszewicz
ROOM ACTIVITIES

Storage of various reagents at + 4⁰C and – 20⁰C.
RULES

General laboratory rules
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Training responsible
person
Notes
No
RISK ANALYSIS
Risk
Precaution
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

No
59
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 2U68: MOLECULAR DYNAMICS STORM 860
Room responsible person: Tor Gjøen tlf.44943
ROOM ACTIVITIES:
”Phosphor Imager” cassette reading and blot scanning (chemoluminescence or fluorescence (quantifying
western, northern and southern blots).
RULES:
- New users have to contact Tor Gjøen for training and a review of the procedures.
- You have to keep your own cassettes. They are expensive and are easily broken.
- Makes sure that western/southern blot are wrapped in plastic before they are put in the cassette or are
scanned.
- Refer to the instrument manual for information on functions, sensitivity and use of the scanner before
you plan to scan your plots. You will find the manual at the instrument.
- Do not scan with higher resolution than 200 µm. Your data files will get bigger, but they will not give
you better quality.
- Log on and save your scans on your own directory.
Install the image editing software on your own computer, and edit your images in your office.
INSTRUMENTS:
Fume cupboard with a drain
Molecular Dynamics Storm 860 scanner
Computer
Lamp for resetting cassettes
Instrument responsible person: Tor Gjøen
60
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 1U51: COURSE ROOM
(Last update 21.06.2010)
Room responsible person:
Training responsible person:
Ewa Jaroszewicz
Course supervisor/ Engineer
ROOM ACTIVITIES

Courses
RULES

General laboratory rules
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Various equipment.
Ewa Jaroszewicz
Training responsible
person
Cours supervisor/
Engineer
Notes
RISK ANALYSIS
Risk
Work with bacteria and fungi.
Exposure to harmful chemicals.
Work with an open gas flame.
Precaution
Use safety equipment: lab coat. Gloves and googgles
are used when work requires that.
Clean up spillage of bacteria or fungi with 70 % EtOH
Use safety equipment. Work in the fume hood.
Read Safety Data Sheets in advance.
Students must familiarize themselves with
placement of fire equipment, fire alarms,
emergency exits, emergency showers.
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

Emergency shower

Fire equipment

Fire blanket

Eye wash bottles
61
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 1U53: CENTRIFUGE/BACTERIA GROWING ROOM
(Last update 21.06.2010)
Room responsible person:
Training responsible person:
Ewa Jaroszewicz
Ewa Jaroszewicz
ROOM ACTIVITIES


Centrifugation
Growing of bacteria
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Centrifuge, Beckman
Table incubator, New
Brunswick Scientific
Ewa Jaroszewicz
Training responsible
person
Ewa Jaroszewicz
Ewa Jaroszewicz
Ewa Jaroszewicz
Notes
RISK ANALYSIS
Risk
Exposure to spore-forming bacteria and fungi
Precaution
Use safety equipment.
Clean up spillage of bacteria with 70 % EtOH.
All bacterial waste must be autoclaved.
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

62
No
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 1U53: COOLING ROOM
Room responsible person: Mona Gaarder, tlf. 44945/28
ROOM ACTIVITIES:
- Storing material for lab course use.
RULES
- Keep things in order.
63
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 1U62: LAB COURSE PREPARATION ROOM
Room responsible person:
Training responsible person:
Mona Gaarder
Mona Gaarder
ROOM AVTIVITIES

Lab course preparation: Cultivation of bacteria and fungi.
RULES

General laboratory rules
INSTRUMENTS AND EQUIPMENT
Name/type
PFGEInstrument: Bio-Rad
CHEF-DR III system,
Beckman GeneLine
Responsible person
Training responsible person
Ewa Jaroszewicz
Ewa Jaroszewicz
Notes
RISK ANALYSIS
Risk
Preventive measure
Pathogenic bacteria
Use a labcoat and gloves. Avoid any spillage.
Use a labcoat and gloves, and a face mask
Be careful when you use it
Fungi
Gas jet
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY


64
Eye washer
First aid kit
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 1U64: POLAROID CAMERA ROOM (NOT NAMED) BY THE ELEVATOR
Room responsible person:
Training responsible person:
Ole Andreas Løchen Økstad
Ole Andreas Løchen Økstad
ROOM ACTIVITIES



Photographing (documentary proof)of results at visible light.
Photographing (documentary proof)of results at UV light.
RULES




Tidy up!
Clean the light box with alcohol when you are finished.
Do not use the equipment until you are fully trained
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Polaroid camera and UV light box
Ole Andreas Løchen Økstad
Training responsible
person
Notes
RISK ANALYSIS
Risk
Preventive measure
Do not expose your skin or eyes to UV light
Wear a face shield, gloves and a lab coat.
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

None
65
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 1U65: WEIGHING ROOM
(Last update 21.06.2010)
Room responsible person:
Training responsible person:
Ewa Jaroszewicz
Ewa Jaroszewicz
ROOM ACTIVITIES




Storage of chemicals
Making of solutions and media
pH-measure
Storage of Safety Data Sheets
RULES

General laboratory rules
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
pH-meter, 744 Metroholm
Weights
Heating plates
Ewa Jaroszewicz
Ewa Jaroszewicz
Ewa Jaroszewicz
Training responsible
person
Ewa Jaroszewicz
Ewa Jaroszewicz
Ewa Jaroszewicz
Notes
RISK ANALYSIS
Risk
Exposure to harmful chemicals.
Precaution
Read Safety Data Sheets in advance.
Use safety equipment when handling chemicals.
Work in fume hood.
Clean up spillage of chemicals immediately.
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

66
No
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 1U67: MEDIUM CULTIVATION ROOM
Room responsible person:
Training responsible person:
Mona Gaarder
Mona Gaarder
ROOM ACTIVITIES



Lab couse preparation
Manufacturing of cultivation dishes
Preparations before autoclavation
INSTRUMENTER/UTSTYR
Name/type
Responsible person
Mona Gaarder
Dry sterilization unit
Mona Gaarder
Water bath
Mona Gaarder
Micro wavw oven
Mona Gaarder
Beckman spectophotometer Mona Gaarder
Genius PCR machine
Mona Gaarder
Disc distributing machine
Training responsible person
Notes
Mona Gaarder
Mona Gaarder
Mona Gaarder
Mona Gaarder
RISK ANALYSIS
Risk
None
Preventive measure
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY



Eye washer
Fire extinguisher
67
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 1U69: AUTOCLAVE/CLEANING ROOM
Room responsible person:
Training responsible person:
Mona Gaarder
Mona Gaarder
ROOM ACTIVITIES


Cleaning equipment in the lab washer.
Autoclavation of equipment, fluids and bacterial waste..
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Mona Gaarder
Autoclave small. HMC
Mona Gaarder
2 lab washers. Miele
Mona Gaarder
MQ og RO water purifying equipment Mona Gaarder
Autoclave large. Thune
Training responsible
person
Mona Gaarder
Mona Gaarder
Mona Gaarder
Mona Gaarder
Millipore
RISK ANALYSIS
Risk
Preventive measure
Pathogenic bacteria
Wear a labcoat and gloves. Avoid any spillage.
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

68
Eye washer
Notes
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 151: CELL CULTIVATION LABORATORY
Room responsible person:
Training responsible person:
Mona Gaarder
ISA group: Anne-Lise Rishovd/Tor Gjøen
Neuro group: Mona Gaarder/Ragnhild Paulsen
ROOM ACTITIVIES



Cultivation of virus from fish
Cultivation of cell lines
Preparation of primary cultures
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Termo Forma cupboard with Hepafilter and
CO2.
Forma Scientic cupboard with CO2
Mona Gaarder og Ragnhild
Paulsen
Incubator for fish 15o og 20o degrees centigrade,
Termaks
Water bath: Grant
Anne-Lise Rishovd og
Tor Gjøen
Mona Gaarder
Mona Gaarder/ Anne-Lise
Rishovd
Mona Gaarder
LAF-Bench x 2: Holten Safe 2000
Nikon TMS Microscope
Training responsible
person
M. Gaarder og
R.Paulsen
Note
s
A.L.Rishovd og T.
Gjøen
M. Gaarder
M.Gaarder/A.L.Rishovd
M.Gaarder
RISK ANALYSIS
Risk
Pressureized
gas
Preventive measure
Gas jet
Be careful when you use it
Read the safety data sheet before handling chemicals. Always wear a lab coat and gloves. If
necessary, also use protective goggles and a face mask.
Use a lab coat and gloves
Chemicals
Biological
material
The cylinders are securely tightened
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY


Fire extinguishing equipment
Eye washer
69
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 152: COLD ROOM
Room responsible person:
Training responsible person:
Anne-Lise Rishovd
Research groups
ROOM ACTIVITIES



Reasearch activites
Store room
N2 tank
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Training responsible
person
BioRad Power Pac 300
Anne-Lise Rishovd
Research groups
Horisontal shaker GFL
3005
Anne-Lise Rishovd
Research groups
Roller Mixer SRT2
Stuart Scientific
Anne-Lise Rishovd
Research groups
Centrifuge Wifug
Anne-Lise Rishovd
Research groups
Roller Labinco
Anne-Lise rishovd
Research groups
LS4800 Taylor
Wharton Nitrogen tank
Mona Gaarder
Mona Gaarder
Comments
RISK ANALYSIS
Risk
Fluid nitrogen
Methanol contaning solutions
In general
HSE INSTALLATIONS

70
Safety equipment for handling of nitrogen
Preventive measure
Protective plexiglas mask, insulated gloves, coat
Nitril gloves,protective glasses
GLP (Good Laboratory Pratice)
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 153: MICROSCOPY ROOM
Room responsible person:
Training responsible person:
Karen A. Boldingh Debernard
Each research group
ROOM ACTIVITIES

Microscopy
RULES




You are not allowed to use the instruments without any training.
Do not fix anything if you do not know what it is.
See the instructions at the microscopy room for lighting and extinction of UV-lamps, cleaning of the lenses and
so on
As for confocal – see separate instruction explaining how to switch the instruments on and off.
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Nikon Eclipse TE300 microscope with
Intensilight C-HGFI
Ragnhild Paulsen
/Tor Gjøen
Nikon Elipse TE 2000-E microscope
with D-Eclipse C1 (confocal),
3 lasers
Ragnhild Paulsen
/Tor Gjøen
Training responsible
person
Each research group
INU Tokai hit
TMC air table
Gas cylinders
Labophot-2 fluorescence mikroskop
Comments
Training course
or similar type of
experience is
necessary before
you are allowed
to use the
confocal
microscope.
Ida Hegna/Ewa
Jaroszewicz
RISK ANALYSIS
Risk
Preventive measure
As a general rule there is a low risk
of being injured when the
equipment in this room is used as it
is meant to be.
The confocal microscope uses lasers
in class 3B.
Authorized users only are allowed to use the instrument. Never look
into the laser beam. Do not detach anything. Cool the lasers after use.
Mercury lamps are used for UV
light.
Never look into the UV light. Refer to the separate instruction on
replacing lamps, working hours etc. In the case of an accident – pull
out the plug, leave the room, ventilate thoroughly for at least 30
minutes before tidying.
Pressureized gas cylinders
The cylinders are securely tightened to the wall
71
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 155: RESEARCH LABORATORY
Room responsible person:
Training responsible person:
Hanne C. Winther-Larsen
Hanne C. Winther-Larsen/Anne-Lise Rishovd
ROOM ACTIVITIES



Research activitites
RNA work
Use of instruments
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Mini centrifuge 7M
Hanne Winther-Larsen
Mini centrifuge Galaxy
ministar
Hanne Winther-Larsen
Incubator Innova 4230
Hanne Winther-Larsen
Fume hood
Hanne Winther-Larsen
Gel-electrophoreses for
RNA in fume hood
Ewa Jaroszewicz
Bosch -20° C freezer
Mona Gaarder
Combi fridge/freezer
Hanne Winther-Larsen
Simens - 20° C freezer
Anne-Lise Rishovd
Training responsible person
Hanne Winther-Larsen/ AnneLise Rishovd
Hanne C. Winther-Larsen/ AnneLise Rishovd
Hanne C. Winther-Larsen/ AnneLise Rishovd
Hanne C. Winther-Larsen/ AnneLise Rishovd
Ewa Jaroszewicz /
Research groups
Comments
Mona Gaarder
Hanne C. Winther-Larsen/ AnneLise Rishovd
Anne-Lise Rishovd
RISK ANALSIS - GENERAL LABORATORY RULES
Risk
Chemicals
Biological material
Preventive measure
Read Safety Data Sheet in advance. Use lab coat and
glows. Protective glasses and mouth and nose protection
if necessary. Work in fume hood if necessary. Clean up
spillage of chemicals immediately.
Use lab coat and glows. Avoid spillage
Use with caution. Ensure that the gas burners
Gass and gassburner in fume hood
HSE INSTALLATIONS
 EYE WASH BOTTLE
72
work properly. They must not burn
unnecessary, and they shall be closed when
leaving the laboratory.
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
(Last template update: 05.11.2010)
BL21 156: CENTRIFUGE ROOM
Room responsible person:
Training responsible person:
Anne-Lise Rishovd
Reseach groups and Tor Gjøen
ROOM ACTIVITIES

Use of centrifuges
INSTRUMENTS AND EQUIPMENT
Name/type
Beckman Avanti J-25
High Speed
Sorval RC 3C Plus Low
Speed
Sorval Discovery 100
Ultrasentrifuge
Sorval Crimper
Responsible person
Training responsible
person
Anne-Lise Rishovd
Reseach groups
Anne-Lise Rishovd
Reseach groups
Anne-Lise Rishovd
Tor Gjøen
Anne-Lise Rishovd
Reseach groups
Comments
RISK ANALYSIS
Risk
Heavy equipment
Spillage of bacterial culture
Preventive measure
Careful while carring rotor. Do not carry if you have a
troublesome back.
Avoid spillage. Handling spillage with disinfectant
thoroughly and clean all surfaces.
HSE INSTALLATIONS

None
73
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
(Last template update: 05.11.2010)
BL21 163A: FILM DEVELOPING ROOM
Room responsible person:
Training responsible person:
Anne-Lise Rishovd
Anne-Lise Rishovd
ROOM ACTIVITIES


Develop films
Use of UV box
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
AGFA Curix 60
UV Translink 20.S
Anne-Lise Rishovd
Anne-Lise Rishovd
Training responsible
person
Comments
Anne-Lise Rishovd
Reseach groups
RISK ANALYSIS
Risk
Chemical solutions
UV
HSE INSTALLATIONS

74
Protective plexiglas mask
Preventive measure
Use safety glasses handling chemicals, and rubber
gloves
Use safety glasses and gloves, no bare skin while
cutting gels.
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 163B: ELECTROPHORESIS LABORATORY
Room responsible person:
Training responsible person:
Mona Gaarder
Mona Gaarder
ROOM ACTIVITIES

DNA separation using normal electrophoresis
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Mona Gaarder
Pharmacia Elektrophoresis equipment Mona Gaarder
Power Supply Biorad
Mona Gaarder
EtBr-sensor Vilber Lourmat
Mona Gaarder
Easy-Cast Elektrophoresis system
Training responsible
person
Comments
Mona Gaarder
Mona Gaarder
Mona Gaarder
Mona Gaarder
Light box
RISK ANALYSIS
Risk
Preventive measure
Etbr (may cause mutation)
Wear protective goggles, labcoat and gloves
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

Eye washer
75
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 164: PROTEIN LABORATORY
Room responsible person:
Training responsible person:
Mona Gaarder
Mona Gaarder
ROOM ACTIVITIES



Moulding, running and blotting acrylic amide gels
Weighing
Use of microwave oven
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Training responsible person
Scale AND
Mona Gaarder
Mona Gaarder
Mona Gaarder
Mona Gaarder
Mona Gaarder
Mona Gaarder
Mona Gaarder
Mona Gaarder
Scale METTLER
Heating block
Shaker
Biorad powerpac 300
Microwave oven
Whirlpool
Comments
Mona Gaarder
RISK ANALYSIS
Risk
Preventive measure
Acrylic amide
Wear protective goggles, labcoat and gloves. Work in the fume hood.
Wear nitrile gloves and labcoat. Work in the fume hood.
Buffer
containing
methanol
Chemicals
Read the safety data sheet before handling chemicals. Always wear a lab coat and gloves. If
necessary, also use protective goggles and a face mask.
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY


76
Fume cupboard
Eye washer
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 166: RESEARCH LABORATORY
Room responsible person:
Training responsible person:
Mona Gaarder
Mona Gaarder
ROOM ACTIVITIES

Research work
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Training responsible person
Centrifuge Biofugo pico
Mona Gaarder
Mona Gaarder
Mona Gaarder
Mona Gaarder
Anne-Lise Rishovd
Anne-Lise Rishovd
Mona Gaarder
Mona Gaarder
Mona Gaarder
Anne-Lise Risovd
Anne-Lise Rishovd
Centrifuge Biofugo fresco
Gel doc Biorad
2 water baths
PTC-machine
ABI 6100 Nucleic Acid
Prepstation
ABI 2720 Thermed Cycler
Comments
Anne-Lise Rishovd
Anne-Lise Rishovd
RISK ANALYSIS
Risk
Preventive measure
Gas jet
Be careful in use
Read the safety data sheet before handling chemicals. Always wear a lab coat and gloves. If
necessary, also use protective goggles and a face mask.
Use labcoat and gloves. Avoid any spillage.
Chemicals
Biological
material
Pathogenic
bacteria
Use labcoat and gloves. Avoid any spillage.
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY




Fire blanket and fire extinguisher
Eye washer
Fume hood
Ventilated chemical locker
77
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 167: RESEARCH LABORATORY
(Last update 21.06.2010)
Room responsible person:
Training responsible person:
Ewa Jaroszewicz
Ewa Jaroszewicz
ROOM ACTIVITIES


Research
Use of instruments
RULES

General laboratory rules
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
2 PCR-blocks
3 Table centrifuges, Heraeus
UV-transmission apparatus,
Fluo Link from Vilber
Lourmat
Fume hood, Normodul
Distillation apparatus
Water bath
Microwave
Heating blocks
Ewa Jaroszewicz
Ewa Jaroszewicz
Training responsible
person
Ewa Jaroszewicz
Ewa Jaroszewicz
Ewa Jaroszewicz
Notes
Ewa Jaroszewicz
Ewa Jaroszewicz
Ewa Jaroszewicz
Ewa Jaroszewicz
Ewa Jaroszewicz
Ewa Jaroszewicz
Ewa Jaroszewicz
Ewa Jaroszewicz
Ewa Jaroszewicz
Ewa Jaroszewicz
Ewa Jaroszewicz
RISK ANALYSIS
Risk
Exposure to harmful chemicals.
Work with an open gas flame.
Precaution
Read Safety Data Sheets in advance.
Use safety equipment.
Work in fume hood.
Clean up spillage of chemicals immediately.
Use with coution. Ensure that the gas burners
work properly. They must not burn
unnecessary, and they shall be closed when
leaving the laboratory.
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY




78
Emergency shower
Fire equipment
Fire blanket
Eye wash bottles
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences

(Last template update: 05.11.2010)
BL21 168 INSTRUMENT ROOM
Room responsible person:
Training responsible person:
Anne-Lise Rishovd
Reseach groups
ROOM ACTIVITIES

Use of analytical instruments
INSTRUMENTS AND EQUIPMENT
Name/type
ChemiGenius,Bio
Imaging System
LightCycler 480, qPCR
Galaxy S PLUS,
Picodrop
Responsible person
Training responsible
person
Anne-Lise Rishovd
Reseach groups
Anne-Lise Rishovd
Reseach groups
Anne-Lise Rishovd
Reseach groups
Comments
RISK ANALYSIS
Risk
None in particular
Preventive measure
GLP
HSE INSTALLATIONS

None
79
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 169: ELECTROPHORESIS ROOM
(Last update 21.06.2010)
Room responsible person:
Training responsible person:
Ewa Jaroszewicz
Ewa Jaroszewicz
ROOM ACTIVITIES



Electroforese
Drying of acrylamide gels
Nitrogen flask for AFM-work
RULES

General laboratory rules
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Electroforese equipment
Gel drying apparatus
Ewa Jaroszewicz
Ewa Jaroszewicz
Ida K. Hegna/
Ewa Jaroszewicz
Nitrogen flask
Training responsible
person
Ewa Jaroszewicz
Research group
Ida K. Hegna/
Ewa Jaroszewicz
Notes
RISK ANALYSIS
Risk
Exposure to Ethydium bromide, acrylamid
Nitrogen gas under pressure
Precaution
Read Safety Data Sheets in advance.
Use saefty equipment. EtBr is mutagenic.
Nitrogen flask must be properly secured.
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

80
No
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL21 170: P-2 ROOM
(Last update 01.11.2010)
Room responsible person:
Training responsible person:
Ewa Jaroszewicz
Ewa Jaroszewicz
ROOM ACTIVITIES



Growing of spore-forming bacteria
Electroporation
OD-measure
RULES


General laboratory rules
Procedures for handling of spore forming bacteria
INSTRUMENTS AND EQUIPMENT
Name/type
LAF-bench, Holten Lamin
Air
Spectrofotometer,
Eppendorf
Various eqipment
Equipment for
electroporation from BioRad
2 CO2 incubators
2 Incubators
Responsible person
Training responsible
person
Ewa Jaroszewicz
Ewa Jaroszewicz
Ewa Jaroszewicz
Ewa Jaroszewicz
Ewa Jaroszewicz
Ewa Jaroszewicz
Mona Gaarder
Mona Gaarder
Ewa Jaroszewicz
Ewa Jaroszewicz
Ewa Jaroszewicz
Ewa Jaroszewicz
Notes
RISK ANALYSIS
Risk
Exposure to spore-forming bacteria.
Precaution
Use safety equipment.
Work in LAF-bench.
Clean up workplace before and after with 70 % EtOH.
Liquid bacteria waste must be autoclaved.
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

No
81
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
CLEANING PROCEDURES IN THE THIRD FLOOR
We have now decided to combine the monitor- and cleaning duty lists for the cell lab. Therefore, two and two
of us work together in doing both tasks in a two-week period. Make sure that each and everyone notices their
cleaning duty weeks! If needed, you can change places with eachother internally!
After laboratory work the users must tidy up in the lab and put the dishes in tubs and bowls.
Drawing ink labels should be removed with technical acetone.
Any specific dishes are addressed by the users themselves.
The dishes from the laboratories number 407, 409, 411, 475, 477 and 483 are to be put in tubs.
The person who has the cleaning job that week, brings these dishes to room 409 where it is washed in the lab
washer.
When this is done, the dishes is placed in drying baskets in room 409.
When the dishes are dry, their openings are covered with tinfoil, and the glassware is sorted and placed in the
cupboards in room 409.
Pipette tips have to be filled up by the one who empties the box.
Pipette tips for autoclavation should be placed in a cupboard in room 477.Tips that fits these boxes can be
found in the drawers there.
Old adhesive tape must be removed and a new one is put on.
Empty glasses for eppendorf tubes that are going to be autoclaved must be filled up. You will find the
eppendorf tubes in room 477.
ALWAYS WEAR GLOVES, AND MAKE SURE THE WORKING SURFACE IS CLEAN WHEN
YOU FILL UP BOXES WITH TIPS AND GLASSES WITH EPPENDORF TUBES.
Autoclavation of tips, eppendorf tubes and the metal tubes for the Pasteur pipettes is done by the persons who
have their cleaning week duty. If you need training in using the autoclave, ask one of the engineers at the
department.
Equipment that is autoclaved should be placed in the cupboard in room 477.
Autoclaved Pasteur pipettes should be put in the cupboard in the cell lab.
 Also remember to bring hazardous waste to room 025 in the basement when the box is filled up. Do
not leave it in the corridor in the third floor.
 Polystyrene boxes should be disposed of in the container at the Department of Chemistry.
 Glass waste is disposed of by one of the engineers when it is needed.
The cleaning week duties are as follows:
Collect the dishes in the tubs, start the lab washer, put the dishes on the drying racks, put on tinfoil, and
place the dried equipment in the cupboards in room 409.
The chemicals and the scales must be kept in order.
Autoclaving various equipment.
Waste disposal and making ready with new waste boxes.
A list with the cleaning week shift rotation should be placed in the washing room and in the cell lab. The
list should be signed when the work is done. The tasks in the cell lab is described outside the door in the
cell lab.
82
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
(Last template update: 08.11.2010)
BL25 310: NITROGEN TANK IN COLD STORAGE
Room responsible person:
Training responsible person:
Siri Johannesen, phone 56558
Siri Johannesen
ROOM ACTIVITIES

Freezing of cells
INSTRUMENTS AND EQUIPMENT
Name/type
Locator JR-Cryo Biological
Storage SystemsXxxx
Thermo Liquidnitrogen
monitor
Responsible person
Training responsible
person
Comments
Siri Johannesen
Siri Johannesen
RISK ANALYSIS
Risk
Tubes may explode when taken out of the cryo
Preventive measure
Use mask and mittens when you fetch out the cells
HSE INSTALLATIONS
 NO
83
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
(Last template update: 12.11.2010)
BL25 407: ISOTOPE LABORATORY 1
Room responsible person:
Training responsible person:
Camilla Stensrud
Arild Rustan
ROOM ACTIVITIES



Radioactive isotopes are measured in a scintilation countermachine.
Sonication
Use of utrationbath
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Training responsible
person
Scintillation counter,
1450 Microbeta plus
Scintillation, Betacounter,
model TRI CARB 1900
TR
Ultrasonic Processor
Ultrabad, Sonorex AK
100
Ultrabad, Bransonic 220
Termax, serie 4000
Laserprinter
Refridigator/ freezer
Arild Rustan
Arild Rustan
Arild Rustan
Arild Rustan
Engineer
Engineer
Engineer
Engineer
Engineer
Engineer
Hilde Nilsen
Engineer
Engineer
Engineer
Comments
K 8, F 9 og F 10
RISK ANALYSIS
Risk
Preventive measure
Isotopes – radiation ( 3H and
14C)
Special procedure for working with isotopes
HSE INSTALLATIONS





84
Riskwaste
Separat riskwaste for radioactiv waste.
Eyewash bottle
Fire extinguisher
Fire blanket
Use googels and glows. Every user has to attend an
aproved course in Radiation protection and
radioactivity.
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
(Last template update: 01.12.2010)
BL25 408: EXTRACTION/THIN LAYER ROOM
Room responsible person:
Training responsible person:
Arild Rustan, phone 56558
Arild Chr. Rustan
ROOM ACTIVITIES


Extraction of lipids
Thin layer chromography
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Gas bottle
Termaxs Incubator Cabinet
Arild Rustan
Arild Rustan
Training responsible
person
Arild Rustan
Arild Rustan
Comments
RISK ANALYSIS
Risk
Isotop, radiation hazard
Solvents
Preventive measure
All users must complete an approved course
Work in the safety bench, use gloves
HSE INSTALLATIONS
 EYE WASH BOTTLE
 FIRE EXTINGUISHER
85
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
(Last template update: 03.11.2010)
BL25 409: CHEMICAL/WEIGHT ROOM
Room responsible person:
Training responsible person:
Hilde Nilsen, phone, 56547
Hilde Nilsen
ROOM ACTIVITIES



Making solutions
pH adjustment
Chemical registration
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Training responsible
person
Sartorius basic x2
Sartorius 1205 P
Ohaus Model TS 120S
Mettler Toledo
pH- meter,PHM 210
Miele oppvaskmaski
Hilde Nilsen
Hilde Nilsen
Hilde Nilsen
Hilde Nilsen
Hilde Nilsen
Hilde Nilsen
Hilde Nilsen
Hilde Nilsen
Hilde Nilsen
Hilde Nilsen
Hilde Nilsen
Hilde Nilsen
RISK ANALYSIS
Risk
Chemicals
HSE INSTALLATIONS
 EYE WASH BOTTLE
 FIRE EXTINGUISHER
86
Preventive measure
Use mask and gloves
Comments
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
(Last template update: 08.11.2010)
BL25 411: HPLC ROOM
Room responsible person:
Training responsible person:
Siri Johannesen,office 475B, phone 56554
Siri Johannesen
ROOM ACTIVITIES






Sentrifugation
Microsome isolation
Cell experiments
Metabolism experiments
Sample preparation
HPLC
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Training responsible
person
HPLC Perkin Elmer
Optima ultra sentrifuge
Siri Johannesen
Siri Johannesen
Siri Johannesen
Siri Johannesen
Siri Johannesen
Siri Johannesen
Siri Johannesen
Siri Johannesen
Agilent HPLC
Anders Aasberg
Anders Aasberg
HPLC LKB
Supelco Phase extraction
Comments
RISK ANALYSIS
Risk
Chemicals
Preventive measure
Use googles, gloves and back bench
HSE INSTALLATIONS
 FIRE EXTINGUISHER
 FIRE BLANKET
 EYEWASH BOTTLE
87
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
(Last template update: 02.11.2010)
BL25 413E: WESTERN ELECTROPHORESIS LAB
Room responsible person:Hilde Nilsen, tlf 56547
Training responsible person:Hilde Nilsen and Siri Johannesen
ROOM ACTIVITIES




Electropheresis
Casting protein gels
Casting agarose gels
Developing
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Electropheresis equipment
Hoefer gel washer
Hilde and Siri
Hilde and Siri
Training responsible
person
Hilde and Siri
Hilde and Siri
Spectrophotometer
Polaroid gel Kamera
Hilde and Siri
Beata
Hilde and Siri
Mohebi
Comments
RISK ANALYSIS
Risk
Acrylamide
Mercaptoethanol
Eth. bromide
HSE INSTALLATIONS
 EYE WASH BOTTLE
88
Preventive measure
Cast gels in safety bench, use gloves
Pippette in safety bench, use gloves
Pippette in safety bench, use gloves
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
(Last template update: 11.11.2010)
BL25 413J (OUTSIDE): SORVAL ULTRA CENTRIFUGE
Room responsible person:
Training responsible person:
Siri Johannesen, phone 56554
Siri Johannesen
ROOM ACTIVITIES

Sentrifugation
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Training responsible
person
Sorval Ultra PRO 80
Ultrasentrifuge
Siri Johannesen
Siri Johannesen
Comments
RISK ANALYSIS
Risk
Inbalance
Preventive measure
Remember to use adapters, and balance the tubes before
sentrifugation.
HSE INSTALLATIONS
 NO
89
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
(Last template update: 11.11.2010)
BL25 477: DNA/RNA LAB
Room responsible person:
Training responsible person:
Beata Mohebi, phone 56554
Beata Mohebi
ROOM ACTIVITIES







PCR
Realtime PCR
DNA/RNA quantitation
Sentrifugation
RNA isolation
Casting gels
Blood sampling
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
RT-PCR ABI PRISM 7000
Gen Amp PCR systems
9700
Gene Quant UV/Visible
spektrophotometer
Qubit Flurometer
Heraeus Fresco 21
sentrifuge
Hettich Universal sentrifuge
Jouan sentrifuge
Techni Dri Block
Beata Mohebi
Training responsible
person
Beata Mohebi
Beata Mohebi
Beata Mohebi
Beata Mohebi
Beata Mohebi
Hilde Nilsen
Hilde Nilsen
Beata Mohebi
Beata Mohebi
Hilde Nilsen
Beata Mohebi
Beata Mohebi
Hilde Nilsen
Beata Mohebi
Beata Mohebi
Comments
2 rotorer
2 rotorer
RISK ANALYSIS
Risk
Inbalance in the sentrifuge
Bloodsampling
HSE INSTALLATIONS
 EYEWASH BOTTLE
 FIRE EXTINGUISHER
90
Preventive measure
The tubes must be in balance before sentrifugation
Contagues materials
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
(Last template update: 02.11.2010)
BL25 480: ISOTOPIC LABORATORY
Room responsible person:
Training responsible person:
Arild Chr Rustan, phone 56558
Arild Chr Rustan
ROOM ACTIVITIES




Work with radioactive sources
Storage of radioactove isotopes
Laboratory is approved as a type C laboratory
Anyone working with radioactive isotopes shall be completed before start working with radiation
and radioactivity
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Sentrifuge: Sigma 2-15X
Arild Rustan
Training responsible
person
Arild Rustan
Arild Rustan
Arild Rustan
Arild Rustan
Arild Rustan
Incubator cabinet, Jouan
IGO 150W/ gas sylinder
Seiwa Optical Tissue
culture microscope,STC-BI
Comments
RISK ANALYSIS
Risk
Isotopes
Preventive measure
All users must complete an approved course
Gas under pressure
HSE INSTALLATIONS
 EYEWASHBOTTLE
 FIRE EXTINGUISHER
 FIRE BLANKET
 GEIGER-MULLER COUNTER
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School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
BL25 481: CELL LABORATORY
Room responsible person:
Training responsible person:
Camilla Stensrud
Engineer or Camilla Stensrud
ROOM ACTIVITIES

The major activity is cellgrowth.
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Training responsible
person
1 Nuire incubator 4500 E,
CO2
1 Nuire incubator 4750 E,
CO2
1 Forma Scientific
incubator, CO2
2 Laf-benches, Holten
Safe 2000
Sentrifuge Kubota 2010
Microscope Leica DMIL
Water bath
Camilla Stensrud
Engineer
Camilla Stensrud
Engineer
Camilla Stensrud
Engineer
Camilla Stensrud
Engineer
Camilla Stensrud
Camilla Stensrud
Camilla Stensrud
Camilla Stensrud
Engineer
Engineer
Engineer
Engineer
Refrigerator and freezer
Comments
18K, 19 K
RISK ANALYSIS
Risk
Preventive measure
Biological material- may be contagious
Working with virus
Gas cylinder
Gas flame
Filling the N2-freezer
Use glows, a special coat and shoes.
HSE INSTALLATIONS



92
Riskwaste
Eyewash bottle
Fire blanket
Special procedyre
The cylinder is secured
Use carefully
Heavy lifting
School of Pharmacy HSE Manual: Department of Pharmaceutical Biosciences
(Last template update: 08.11.2010)
BL25 484: MICROPLATE READER AND PROTEIN CHROMOGRAPHY
Room responsible person:
Training responsible person:
Harald Thidemann Johansen, phone 56595
Harald Thidemann Johansen
ROOM ACTIVITIES



For quantitative detection of light emitting and light absorbing marker
Detection of protein and enzymes
Protein chromatography
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Training responsible
person
FPLC Chromography
Victor 3 – 1420
Multilabel counter
HTJ
HTJ
HTJ
HTJ
Comments
reservation necessary
RISK ANALYSIS
Risk
Solvents
Preventive measures
Work in safety bench
Chemicals
Use glasses and gloves
HSE INSTALLATIONS
 EYEWASH BOTTLE
93
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
ROUTINES AT THE DEPARTMENT OF PHARMACEUTICAL
CHEMISTRY
HSE
Health, Safety and the Environment in the
workplace
Version 2010
Universitety of Oslo
School of Pharmacy
Department of Pharmaceutical Chemistry
94
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL23 Ø182B: Laboratory ............................................................................................................................97
BL23 Ø184: Laboratory ...............................................................................................................................98
BL24 ØK19A: Instrument room...................................................................................................................99
BL24 ØK82: Chemicals Storage Room ...................................................................................................... 100
BL24 Ø187: Students’ courses laboratory .................................................................................................. 101
BL24 Ø188: Rotavapor room ..................................................................................................................... 102
BL24 Ø191: Master students’ laboratory .................................................................................................... 103
BL24 Ø192: Laboratory ............................................................................................................................ 104
BL24 Ø196 : LC/MS laboratory................................................................................................................. 105
BL24 Ø197: Laboratory ............................................................................................................................. 106
BL24 Ø198: Laboratory ............................................................................................................................. 107
BL24 Ø199: Ph. D students’ lab ................................................................................................................. 108
BL24 Ø282 : Students’ courses laboratory ................................................................................................. 109
BL24 Ø283: Rotavapor room ..................................................................................................................... 110
BL24 Ø 284: Washing room/storage room ................................................................................................. 111
BL24 Ø285 A: Chemicals Storage Room /IR-room .................................................................................... 112
BL25 018: Mill room ................................................................................................................................. 113
BL25 037: Storage room ............................................................................................................................ 113
BL25 202: HPLC laboratory ...................................................................................................................... 114
BL25 203: LCMS laboratory ..................................................................................................................... 115
BL25 203: Capillary electrophoresis laboratory.......................................................................................... 116
BL25 207: Students’ lab courses laboratory ............................................................................................... 117
BL25 212: Balance room ........................................................................................................................... 118
BL25 214: Students courses’ laboratory ..................................................................................................... 119
BL25 217: Laboratory................................................................................................................................ 120
BL25 220: Conference room ...................................................................................................................... 121
BL25 222: Laboratory................................................................................................................................ 122
BL25 227: Conference room ...................................................................................................................... 123
BL25 229: GC/GCMS laboratory............................................................................................................... 124
BL25 314: Cooling room ........................................................................................................................... 125
BL25 316: Research laboratory .................................................................................................................. 126
BL25 318: Instrument laboratory ............................................................................................................... 127
BL25 330: Kitchen/office .......................................................................................................................... 128
BL25 331: Laboratory................................................................................................................................ 129
BL25 333: Laboratory................................................................................................................................ 130
BL25 334: Instrumentlab. .......................................................................................................................... 131
BL25 336: Drug collection......................................................................................................................... 132
BL25 339: Students courses’ laboratory ..................................................................................................... 133
BL25 339 B: Chromatography lab.............................................................................................................. 134
95
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 340: Preparation room/courses ......................................................................................................... 135
BL25 344: Laboratory ............................................................................................................................... 136
BL25 358: Balance room ........................................................................................................................... 137
BL25 362: Laboratory ............................................................................................................................... 138
BL25 365: Conference room ...................................................................................................................... 139
96
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL23 Ø182B: LABORATORY
Room responsible person:
Training responsible person:
Jo Klaveness
Jo Klaveness
ROOM ACTIVITIES





Handling of chemicals
Organic synthesis
Preparative chromatography
Use of instruments (rotavapor, vacuum pumps)
Use of gas under pressure (nitrogen and argon)
INSTRUMENTS AND EQUIPMENT
Name/type
2 Rotavapors
2 Scales
1 Membrane pump
1 High vacuum pump
1 UV-lamp
1 Oven
4 Magnetic stirrers w.
temp. control
1 Heater
1 Bunsen burner
1 Fridge/freezer
Responsible
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Responsible for training
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Comments
RISK ANALYSIS
Risk
Accidents and injuries due to improper use of
instruments and equipment
Exposure to harmful chemicals
Accidents and injuries due to improper handling of
pressurized gas cylinders.
Preventive measure
Read SOP or user’s manual before use
Always wear eye protective glasses
Consult the material safety data sheets for proper handling of
chemicals
Use personal safety equipment when handling chemicals
Never conduct any experiments outside the fume hoods
Clean up spillage of chemicals immediately
Always store chemicals in the chemical storage locker
Work benches must be cleaned after use
See section “The laboratory: Safety and the working environment”
in this handbook, to ensure correct handling of pressurized gas
cylinders
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY





Emergency shower (to the left outside the door)
Eye wash flasks
First aid kit
Fire extinguisher (CO2) and fire blanket
1 fume hood
97
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL23 Ø184: LABORATORY
Room responsible person:
Training responsible person:
Jo Klaveness
Jo Klaveness
ROOM ACTIVITIES





Handling of chemicals
Organic synthesis
Preparative chromatography
Use of instruments (rotavapor, vacuum pumps)
Use of gas under pressure (nitrogen and argon)
INSTRUMENTS AND EQUIPMENT
Name/type
2 Rotavapors
2 Scales
1 Membrane pump
1 High vacuum pump
1 UV-lamp
1 Oven
4 Magnetic stirrers w.
temp. control
1 Heater
1 Bunsen burner
1 Fridge/freezer
Responsible
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Responsible for training
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Comments
RISK ANALYSIS
Risk
Accidents and injuries due to improper use of
instruments and equipment
Exposure to harmful chemicals
Accidents and injuries due to improper handling of
pressurized gas cylinders.
Preventive measure
Read SOP or user’s manual before use
Always wear eye protective glasses
Consult the material safety data sheets for proper handling of
chemicals
Use personal safety equipment when handling chemicals
Never conduct any experiments outside the fume hoods
Clean up spillage of chemicals immediately
Always store chemicals in the chemical storage locker
Work benches must be cleaned after use
See section “The laboratory: Safety and the working environment”
in this handbook, to ensure correct handling of pressurized gas
cylinders
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY





98
Emergency shower (to the right outside the door)
Eye wash flasks
First aid kit
Fire extinguisher (CO2) and fire blanket
2 fume hoods
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL24 ØK19A: INSTRUMENT ROOM
Room responsible person:
Training responsible person:
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
ROOM ACTIVITIES



Deposit and withdrawal of organic solvents and NMR-solvents
Use of instruments
Solvent drying
RULES
Deposit/withdrawal of the organic and NMR-solvents of most use is exempted from the normal
chemicals registration procedures. Each laboratory is registrated with a certain constant amount of
the organic and NMR-solvents of most use (refer to ECOonline).
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Training responsible
person
NMR-instrument (2-65 MHz):
Bruker MiniSpec
Iuliana Johansen/Atle
Haugen
Pål Rongved
C-system: VersaFlash
Iuliana Johansen
Iuliana Johansen
Sentrifuge: Heraeus Sepatech
Iuliana Johansen/Atle
Haugen
Iuliana Johansen/Atle
Haugen
Water bath w/shaking
Iuliana Johansen/Atle
Haugen
Iuliana Johansen/Atle
Haugen
Water purifying instrument:
ELGA Prima and Maxima
Iuliana Johansen
Iuliana Johansen
Braun Labsonic U ultra sonic
bath
Iuliana Johansen
Iuliana Johansen
Coulter Electronics
rolling/turning robot
Atle Haugen
Atle Haugen
Metrohm 718 Stat Titrino
titrator
Atle Haugen
Atle Haugen
Comments
To prevent any water leakages,
please close the dispenser
properly.
RISK ANALYSIS
Risk
Exposure to harmful
chemicals
Preventive measure
Use personal safety equipment when handling chemicals
Clean up spillage of chemicals immediately
Always store chemicals in the chemical storage locker
Work benches must be cleaned after use
Synthesis products have to be labelled by date, name/producer, product
no../journal no., total amount, chemical name and/or structure.
HSE INSTALLATIONS





Emergency shower (to the left outside the door)
Fire extinguishing equipment and fire blanket
Eye wash flasks
Labelled emergency exit
List of phone numbers at accidents/injuries
99
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL24 ØK82: CHEMICALS STORAGE ROOM
Room responsible person:
Training responsible person:
Atle Haugen
Atle Haugen
ROOM ACTIVITIES


Deposit and withdrawal of chemicals
Local reception of chemical waste
RULES

Deposit, withdrawal and destruction of chemicals has to be registered in accordance with the lists on
the door.
INSTRUMENTS AND EQUIPMENT
Name/type
Training responsible
person
Responsible person
Comments
RISK ANALYSIS
Risk
Exposure to harmful chemicals
Smell and unwanted incidents/accidents in the
corridors
Preventive measure
Use personal safety equipment when handling
chemicals
Clean up spillage of chemicals immediately
Always store chemicals in the chemical storage locker
Work benches must be cleaned after use
Synthesis products have to be labelled by date,
name/producer, product no../journal no., total amount,
chemical name and/or structure.
Containers of chemicals should be transported in secure
vessels or trolleys.
HSE INSTALLATIONS




100
Emergency shower (right outside the door)
Fire extinguishing equipment and fire blanket
Eye wash flasks
Absorbent for spilled chemicals and liquids
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL24 Ø187: STUDENTS’ COURSES LABORATORY
Room responsible person:
Training resposible person:
Trond Vidar Hansen
Trond Vidar Hansen
ROOM ACTIVITIES

Practical teaching of general and organic chemistry for lower-degree students
INSTRUMENTS AND EQUIPMENT
Name/type
4 scales
4 heating ovens
1 UV-lamp
3 melting point analyzers
Atle Haugen
Training
responsible person
Atle Haugen
Atle Haugen
Atle Haugen
Atle Haugen
Atle Haugen
Atle Haugen
Atle Haugen
Responsible person
Comments
Responsible at the Department of Chemistry:
Elhoussine Merrachi
Responsible at the Department of Chemistry:
Elhoussine Merrachi
Responsible at the Department of Chemistry:
Elhoussine Merrachi
Responsible at the Department of Chemistry:
Elhoussine Merrachi
RISK ANALYSIS
Risk
Accidents and injuries due to improper use of instruments
and equipment
Exposure to harmful chemicals
Preventive measure
Read SOP or user’s manual before use
Always wear eye protective glasses
Consult the material safety data sheets for proper handling
of chemicals
Use personal safety equipment when handling chemicals
Never conduct any experiments outside the fume hoods
Clean up spillage of chemicals immediately
Always store chemicals in the chemical storage locker
Work benches must be cleaned after use
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

20 fume hoods

2 emergency showers

2 first aid kits

2 fire extinguishers CO2 34B

2 fire extinguisher Glutex ABC-powder 89B

1 fire blanket

A water fire hose outside each of the entrances to the room

Eye washers at every bench

Absorbent for spilled chemicals and liquids
101
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL24 Ø188: ROTAVAPOR ROOM
Room responsible person:
Training resposible person:
Trond Vidar Hansen
Trond Vidar Hansen
ROOM ACTIVITIES

Solvent evaporation
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible
person
Training
responsible person
4 rotavapors IKA
Atle Haugen
Atle Haugen
2 diaphragm pumps
VacuuBrand
Atle Haugen
Atle Haugen
RISK ANALYSIS
Risk
Accidents and injuries due to improper use of
instruments and equipment
Exposure to harmful chemicals
Comments
Responsible at the Department of Chemistry:
Elhoussine Merrachi
Responsible at the Department of Chemistry:
Elhoussine Merrachi
Preventive measure
Read SOP or user’s manual before use
Consult the material safety data sheets for
proper handling of chemicals
Use personal safety equipment when handling
chemicals
Clean up spillage of chemicals immediately
Work benches must be cleaned after use
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

emergency shower (in the neighbouring room, Ø187)

fire extinguisher CO2 (just outside the door)

fire extinguisher Glutex ABC-powder (just outside the door)

water fire hose (just outside the door)

6 fume hoods

first aid kit
102
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL24 Ø191: MASTER STUDENTS’ LABORATORY
Room responsible person:
Training responsible person:
Trond Vidar Hansen
Trond Vidar Hansen
ROOM ACTIVITIES



Teaching and supervision of master students
Practical organic synthesis as part of master theses
Sample purification using liquid chromatography
INSTRUMENTS AND EQUIPMENT
Name/type
2 rotavapors
Liquid chromatograph-Flash
3 scales
3 heating ovens
2 UV-lamps
4 magnetic stirrers with
heating
2 vacuumpumps
2 cylinders of pressurized
N2, 50 L
1 heating mantle
1 fridge/freezer
Responsible person
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Training responsible person
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Comments
RISK ANALYSIS
Risk
Accidents and injuries due to improper
use of instruments and equipment
Exposure to harmful chemicals
Accidents and injuries due to improper
handling of pressurized gas cylinders.
Preventive measure
Read SOP or user’s manual before use
Always wear eye protective glasses
Consult the material safety data sheets for proper handling of
chemicals
Use personal safety equipment when handling chemicals
Never conduct any experiments outside the fume hoods
Clean up spillage of chemicals immediately
Always store chemicals in the chemical storage locker
Work benches must be cleaned after use
See section “The laboratory: Safety and the working environment” in
this handbook, to ensure correct handling of pressurized gas
cylinders
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY






Emergency showe
Eye wash flasks
Chemizorb (for collecting spillage of chemicals)
Fire blanket
Fire extinguisher (CO2)
7 fume hoods
103
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL24 Ø192: LABORATORY
Room responsible person:
Training responsible person:
Pål Rongved
Pål Rongved
ACTIVITIES:

Common laboratory work
INSTRUMENTS AND EQIUPMENT
Name/type
1 IKA rotavapor
2 IKA magnetic stirrers
2 water jet pumps
1 AGA pressureized argon
cylinder 200 bar / 3000
psi.
1 Thermaks heating oven
1 Ohaus laboratory scale
Responsible
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Responsible for training
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Comments
RISK ANALYSIS
Risk
Accidents and injuries due to improper use of
instruments and equipment
Exposure to harmful chemicals
Accidents and injuries due to improper
handling of pressurized gas cylinders.
Preventive measure
Read SOP or user’s manual before use
Always wear eye protective glasses
Consult the material safety data sheets for proper handling of
chemicals
Use personal safety equipment when handling chemicals
Never conduct any experiments outside the fume hoods
Clean up spillage of chemicals immediately
Always store chemicals in the chemical storage locker
Work benches must be cleaned after use
See section “The laboratory: Safety and the working environment” in
this handbook, to ensure correct handling of pressurized gas cylinders
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY







104
Emergency shower (5 meters to the right outside the door)
Fire extinguisher
Fire blanket
PLUM eye washer
First aid kit
Gas mask
1 fume hood
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL24 Ø196 : LC/MS LABORATORY
Room responsible person:
Training responsible person:
Pål Rongved
Pål Rongved
ROOM ACTIVITIES


Chemical compound analysis by high pressure liquid chromatography/mass spectrometry
Student teaching and instruction
INSTRUMENTS AND EQUIPMENT
Name/type
High pressure liquid
chromatography/mass
spectrometry, Agilent
Nitrogen generator, Agilent
Air compressor, Jun-Air
Vacuum pump, Edwards
Compressed helium, 50 L
cylinder
Responsible person
Training responsible
person
Iuliana Johansen/Atle Haugen
Iuliana Johansen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen
Iuliana Johansen
Iuliana Johansen
Iuliana Johansen/Atle Haugen
Iuliana Johansen
Comments
RISK ANALYSIS
Risk
Accidents and injuries due to improper use of
instruments and equipment
Exposure to harmful chemicals due to insufficient
ventilation
Health injury due to noise
Preventive measure
Read SOP or user’s manual before use
Never conduct any experiments involving open fumes
of harmful chemicals, neither in the fume hoods or in
the room itself
Use protective hearing gear during lasting work in this
room
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY






Emergency shower (to the right outside the door)
Eye wash flasks
Fire extinguisher (CO2)
Fire blanket
2 fume hoods
Air conditioning system
105
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL24 Ø197: LABORATORY
Room responsible person:
Training responsible person:
Trond Vidar Hansen
Trond Vidar Hansen
ROOM ACTIVITIES





Handling of chemicals
Organic synthesis
Preparative chromatography
Use of instruments (rotavapor, vacuum pumps)
Use of gas under pressure (nitrogen and argon)
INSTRUMENTS AND EQUIPMENT
Name/type
1 Rotavapor
1 Scale
1 Vacuum pump
1 Oven
1 UV-lamp
2 Magnetic stirrers w.
temp. control
1 Fridge/freezer
Responsible person
Atle Haugen/Iuliana Johannesen
Atle Haugen/Iuliana Johannesen
Atle Haugen/Iuliana Johannesen
Atle Haugen/Iuliana Johannesen
Atle Haugen/Iuliana Johannesen
Atle Haugen/Iuliana Johannesen
Atle Haugen/Iuliana Johannesen
Training responsible person
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Comments
Atle Haugen/Iuliana Johansen
RISK ANALYSIS
Risk
Accidents and injuries due to improper use of
instruments and equipment
Exposure to harmful chemicals
Sashes of fume hoods may fall down and injure the
person working in front of the hood
Occasional stop of ventilation may cause leak of
toxic vapors from fume hood
Preventive measure
Read SOP or user’s manual before use
Always wear eye protective glasses
Consult the material safety data sheets for proper
handling of chemicals
Use personal safety equipment when handling
chemicals
Never conduct any experiments outside the fume hoods
Clean up spillage of chemicals immediately
Always store chemicals in the chemical storage locker
Work benches must be cleaned after use
Check the steel lines supporting sashes regularly.
Report immediately when there is damage with any
lines and ask for repairing
Be aware of the air flow with the fume hood all the
time. If ventilation stops, lower down the sash and leave
the room.
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY





106
Emergency shower (outside the door to the right)
Eye wash flasks (above the sink)
Fire blanket (“Brannteppe”, above the sink)
Fire extinguisher (CO2) (beside the sink)
3 fume hoods (1 occupied by rotavapor and vacuum pump)
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL24 Ø198: LABORATORY
Room responsible person:
Training responsible person:
Trond Vidar Hansen
Trond Vidar Hansen
ROOM ACTIVITIES





Handling of chemicals
Organic synthesis
Preparative chromatography
Use of instruments (rotavapor, vacuum pumps)
Use of gas under pressure (nitrogen and argon)
INSTRUMENTS AND EQUIPMENT
Name/type
2 Rotavapors
2 Scales
1 Membrane pump
1 High vacuum pump
1 UV-lamp
1 Oven
4 Magnetic stirrers w.
temp. control
1 Heater
1 Bunsen burner
1 Fridge/freezer
Responsible
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Responsible for training
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Atle Haugen/Iuliana Johansen
Comments
RISK ANALYSIS
Risk
Accidents and injuries due to improper use of
instruments and equipment
Exposure to harmful chemicals
Accidents and injuries due to improper handling of
pressurized gas cylinders.
Preventive measure
Read SOP or user’s manual before use
Always wear eye protective glasses
Consult the material safety data sheets for proper handling of
chemicals
Use personal safety equipment when handling chemicals
Never conduct any experiments outside the fume hoods
Clean up spillage of chemicals immediately
Always store chemicals in the chemical storage locker
Work benches must be cleaned after use
See section “The laboratory: Safety and the working environment”
in this handbook, to ensure correct handling of pressurized gas
cylinders
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY





Emergency shower (to the left outside the door)
Eye wash flasks
First aid kit
Fire extinguisher (CO2)
3 fume hoods
107
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL24 Ø199: PH. D STUDENTS’ LAB
(Last template update: 11.05.2010)
Room responsible person:
Training responsible person:
Trond Vidar Hansen
Trond Vidar Hansen
ROOM ACTIVITIES

Synthetic organic chemistry
INSTRUMENTS AND EQUIPMENT
Name/type
2 rotary evaporators
1 membrane pump
VacuuBrand
2 water jet pumps
4 magnetic stirrer plates
UV-lamp
Refrigerator with chemicals
Laboratory scale
2 heating cabinets
50 L Nitrogen pressure tank
50 L Argon pressure tank
Responsible person
Iuliana Johansen/Atle Haugen
Training responsible person
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Iuliana Johansen/Atle Haugen
Comments
RISK ANALYSIS
Risk
Accidents and injuries due to improper use of
instruments and equipment
Exposure to harmful chemicals
Accidents and injuries due to improper handling of
pressurized gas cylinders.
Preventive measure
Read SOP or user’s manual before use
Always wear eye protective glasses
Consult the material safety data sheets for proper handling of
chemicals
Use personal safety equipment when handling chemicals
Never conduct any experiments outside the fume hoods
Clean up spillage of chemicals immediately
Always store chemicals in the chemical storage locker
Work benches must be cleaned after use
See section “The laboratory: Safety and the working environment”
in this handbook, to ensure correct handling of pressurized gas
cylinders
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY






108
Emergency shower (outside the door)
Fire extinguisher
Fire blanket
3 fume hoods
Chemical storage cabinet
Refridgerator
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL24 Ø282 : STUDENTS’ COURSES LABORATORY
Room responsible person:
Training resposible person:
Trond Vidar Hansen
Trond Vidar Hansen
ROOM ACTIVITIES

Practical teaching of general and organic chemistry for lower-degree students
RULES
 Synthesis products have to be labelled by date, name/producer, product no../journal no., total amount, chemical
name and/or structure and should be stored for later use.
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
4 scales
3 heating ovens
1 UV-lamp
2 melting point analyzers
20 magnetic stirrers w/ heating plate
20 heating mantles w/magnetic stirring
40 cupboards of glassware
8 pH - meters
6 desiccators w/vacuum
Iuliana Johansen
Iuliana Johansen
Iuliana Johansen
Iuliana Johansen
Iuliana Johansen
Iuliana Johansen
Iuliana Johansen
Iuliana Johansen
Iuliana Johansen
Training responsible
person
Iuliana Johansen
Iuliana Johansen
Iuliana Johansen
Iuliana Johansen
Iuliana Johansen
Iuliana Johansen
Iuliana Johansen
Iuliana Johansen
Iuliana Johansen
Comments
RISK ANALYSIS
Risk
Accidents and injuries due to improper use of
instruments and equipment
Exposure to harmful chemicals
Preventive measure
Read SOP or user’s manual before use
Always wear eye protective glasses
Consult the material safety data sheets for proper handling of
chemicals
Use personal safety equipment when handling chemicals
Never conduct any experiments outside the fume hoods
Clean up spillage of chemicals immediately
Always store chemicals in the chemical storage locker
Work benches must be cleaned after use
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

20 fume hoods

2 emergency showers

2 first aid kits

2 fire extinguishers CO2 34B

2 fire extinguisher Glutex ABC-powder 89B

1 fire blanket

A fire hose outside each of the entrances to the room

Eye washers at every bench

Absorbent for spilled chemicals and liquids
109
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL24 Ø283: ROTAVAPOR ROOM
Room responsible person:
Training resposible person:
Trond Vidar Hansen
Trond Vidar Hansen
ROOM ACTIVITIES:

Solvent evaporation
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Training responsible person
6 rotavaporr IKA/Büchi
Iuliana Johansen
Supervisor/Iuliana Johansen
1 diaphragm pump
VacuuBrand
Iuliana Johansen
Supervisor/Iuliana Johansen
4 water jet pumps
Iuliana Johansen
Supervisor/Iuliana Johansen
RISK ANALYSIS
Risk
Accidents and injuries due to improper use of
instruments and equipment
Exposure to harmful chemicals
Comments
Preventive measure
Read SOP or user’s manual before use
Consult the material safety data sheets for proper
handling of chemicals
Use personal safety equipment when handling
chemicals
Clean up spillage of chemicals immediately
Work benches must be cleaned after use
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY




110
emergency shower (just outside the door)
fire extinguisher (just outside the door)
fire blanket (just outside the door)
6 fume hoods
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL24 Ø 284: WASHING ROOM/STORAGE ROOM
Room responsible person:
Training responsible person:
Iuliana Johansen
Iuliana Johansen
ROOM ACTIVITIES

Glassware deposit and withdrawal

Use of the lab washer
RULES


The lab washer can be used after reservation or when it is free
Everybody must take away their dishes when it is done
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible
Responsible for training
Industrial washing machine Miele
Iuliana Johansen
Iuliana Johansen
Comments
RISK ANALYSIS
Risk
Exposure to detergents or chemical rests
Preventive measure
Use personal safety equipment when handling chemicals
Clean up spillage of chemicals immediately
Work benches must be cleaned after use
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY




Eye washers
Emergency shower (just outside the door)
Fire extinguisher (just outside the door)
Fire blanket (just outside the door)
111
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL24 Ø285 A: CHEMICALS STORAGE ROOM /IR-ROOM
Room responsible person:
Training responsible person:
Iuliana Johansen
Iuliana Johansen
ROOM ACTIVITIES

Deposit and withdrawal of chemicals and glassware for the lab courses FRM 1000/1010/1030

Use of the IR-instrument
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Iuliana Johansen
PC/ printer connected to the IR-instrument Iuliana Johansen
1 scale
Iuliana Johansen
IR-instrument Perkin Elmer
Training responsible
person
Iuliana Johansen
Iuliana Johansen
Iuliana Johansen
Comments
No hood/short of space
RISK ANALYSIS
Risk
Exposure to harmful chemicals
Preventive measure
Consult the material safety data sheets for proper handling of chemicals
Use personal safety equipment when handling chemicals
Always store chemicals in the chemical storage locker
Work benches must be cleaned after use
Clean up spillage of chemicals immediately
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY




112
Eye washer
Emergency shower (just outside the door)
Fire extinguisher (just outside the door)
Fire blanket (just outside the door)
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 018: MILL ROOM
Room responsible person:
Training responsible person:
Suthajini Yogarajah
ROOM ACTIVITIES

grind raw materials
RULES TO FOLLOW

User must keep order and clean up after themselves.

Use appropriate protective equipment.

The grinders should be cleaned after each use.
Turn off lights when leaving room for the day.

Instruments and equipment
Name/type
Responsible person
BRABENDER Duisburg
Suthajini Yogarajah
Suthajini Yogarajah
ALPINE Augsburg
Training responsible
person
Comments
Freezer
RISK ANALYSIS
Risk
Preventive measure
Harmful noise from instruments
Risk of injury from mechanical, grinding parts
of the mills
Wear ear protection
Be careful while operating the mills
Use appropriate protective equipment.
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY


Fire extinguisher
Ear protection
BL25 037: STORAGE ROOM
Room responsible person:
Training responsible person:
Ellen Hanne Cohen
Ellen Hanne Cohen
Activities
 Storage of different kinds of goods
113
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 202: HPLC LABORATORY
Room responsible person:
Training responsible person:
Leon Reubsaet
ROOM ACTIVITIES

HPLC lab
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
HPLC Dionex Ultimate
HPLC for use in the lab courses
Leon/Stig
Leon
Training responsible
person
Comments
RISK ANALYSIS
Risk
Preventive measure
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY


114
Fire blanket
Eye washer
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 203: LCMS LABORATORY
Room responsible person:
Training responsible person:
Leon Reubsaet
ROOM ACTIVITIES

LCMS lab
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
LCMS Shimadzu 2010 (2stk)
LCMS Finnigan LCQ
Sentrifuge Eppendorf 5804
Vannrenseanlegg Barnstead
Leon
Leon
Leon
Finn
Training responsible
person
Comments
RISK ANALYSIS
Risk
Preventive measure
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY


Fire blanket
Hazardous waste
115
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 203: CAPILLARY ELECTROPHORESIS LABORATORY
Room responsible person:
Training responsible person:
Stig Pedersen-Bjergaard
Stig Pedersen-Bjergaard
ROOM ACTIVITIES


Capillary electrophoresis
Sample processing
RULES

The users must keep things in order, and tidy up.

Material that has been in contact with biological fluids, must be put in the biological waste
box

Switch off the light and lock the door when you leave at the end of the day..
INSTRUMENTS AND EQUIPMENT
Name/type
CE Agilent (2 pcs)
CE Beckman (2 pcs)
HPLC Agilent
Responsible person
Stig PedersenBjergaard
Stig PedersenBjergaard
Stig PedersenBjergaard
Training responsible
person
Stig PedersenBjergaard
Stig PedersenBjergaard
Stig PedersenBjergaard
Comments
RISK ANALYSIS
Risk
Preventive measure
Exposure to harmful biological material
Refer to the chapters on first aid rules and the common
routines for safety and the working environment, and for
decontamination
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY



116
Fire blanket
Fire extinguisher
First aid kit
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 207: STUDENTS’ LAB COURSES LABORATORY
Room responsible person:
Training responsible person:
Finn Tønnesen
ROOM ACTIVITIES

Students lab courses
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
pH meter Metrohm 827
Scale Mettler Toledo
Laboratory washing machine
Ultrasonic bath Branson 5510
Finn
Finn
Finn
Finn
Training responsible
person
Comments
RISK ANALYSIS
Risk
Health hazard by doing mistake while working in a fume
hood
Preventive measure
Refer to the Common Routine chapter – Correct Use of
Fume Cupboards
Risk of water leakages
Turn out the water supply to the water distillating
apparatus at the end of the day.
Do not use the distillating apparatus or the lab washer
after normal working hours.
Exposure to harmful chemicals
Consult the material safety data sheets for proper
handling of chemicals
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY



Fire blanket
Fire extinguisher
Eye washer
117
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 212: BALANCE ROOM
Room responsible person:
Training responsible person:
Finn Tønnesen
ROOM ACTIVITIES

Balance room
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
7 pcs. Mettler AE 200
Finn
Training responsible
person
Comments
RISK ANALYSIS
Risk
Preventive measure
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

118
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 214: STUDENTS COURSES’ LABORATORY
Room responsible person:
Training responsible person:
Finn Tønnesen
ROOM ACTIVITIES

Students courses’ laboratory
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
UV Beckman DU 520
Finn
UV Beckman Du 530
IR Shimadzu IR 408
Finn
Finn
Tablets press
Heating oven
Finn
Finn
Atomic absorption
spectrophotometer
Centrifuge Hettich
Training responsible
person
Comments
Ragnar
Finn
RISK ANALYSIS
Risk
Preventive measure
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

Fire blanket

Fire extinguisher (2 pcs.)

Eye washer
119
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 217: LABORATORY
Room responsible person:
Training responsible person:
ROOM ACTIVITIES

Lab
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Training responsible
person
Comments
RISK ANALYSIS
Risk
Preventive measure
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

Fire blanket

Fire extinguisher

Eye washer
120
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 220: CONFERENCE ROOM
Room responsible person:
Training responsible person:
Finn Tønnesen
ROOM ACTIVITIES

Conference room
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Training responsible
person
Comments
RISK ANALYSIS
Risk
Preventive measure
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

121
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 222: LABORATORY
Room responsible person:
Training responsible person:
ROOM ACTIVITIES

Lab
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Atomic absorption spectrophotometer
Ragnar
Training
responsible person
Comments
RISK ANALYSIS
Risk
Preventive measure
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

Fire blanket

Fire extinguisher
122
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 227: CONFERENCE ROOM
Room responsible person:
Training responsible person:
Finn Tønnesen
ROOM ACTIVITIES

Conference room
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Training responsible
person
Comments
RISK ANALYSIS
Risk
Preventive measurement
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

123
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 229: GC/GCMS LABORATORY
Room responsible person:
Training responsible person:
Finn Tønnesen
ROOM ACTIVITIES

GC/GCMS lab
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
GC Fison (8000 series)
GC Shimadzu 2010
GC Varian Star 3400CX
GCMS Fison MD800
GCMS Shimadzu 2010
Finn
Finn
Finn
Finn
Stig/Finn
Training responsible
person
Comments
RISK ANALYSIS
Risk
Preventive measure
Leakage of combustible gases from gas cylinders. Risk of
explosion at a fire.
Refer to the Laboratory: Safety and the working
environment chapter for proper use and handling of
pressureized gas cylinders.
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

Fire blanket

Fire extinguisher

Eye washer
124
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 314: COOLING ROOM
Room responsible person:
Training responsible person:
Suthajini Yogarajah
Supervisor
ROOM ACTIVITIES



Storage of the gel filtration columns
Kit and special substances
freezing in liquid nitrogen
RULES TO FOLLOW
 User must keep order and clean up after themselves.
 Chemical spills should be removed immediately.
 Turn off lights when leaving room for the day.
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Liquid nitrogen tank N2
Siri Johannesen 4.
etg
Training responsible
person
Comments
Cradle
RISK ANALYSIS
Risk
Preventive measure
Risk of suffocation by emissions in confined
spaces
Be careful when working with and filling of
liquid nitrogen.
Wear eye protection and appropriate gloves
Need regular maintenance and check leakage
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY



Fire extinguisher
Fire Blanket
Eyewash bottles
125
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 316: RESEARCH LABORATORY
Room responsible person:
Training responsible person:
Hilde Barsett
Supervisor
ROOM ACTIVITIES


Research
Use of instruments
REGLER





The users must keep things in order and tidy up.
The users must remove paper and glass waste when it’s needed.
Fume cupboards that are not used shall have their hatches closed.
The lab scales must be cleaned after use.
Switch off the light in the end of the day.
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Rotavapor IKA , VWR
Fraction collector
Phenol/sulfuric acid test
equipment
Suthajini Yogarajah
Ellen Hanne Cohen
Training responsible
person
Comments
Laboratory scale
RISK ANALYSIS
Risk
Accidents and injuries due to improper use of
instruments and equipment
Exposure to harmful chemicals
HMS – INSTALLASJONER





Emergency shower (in the vicinity)
Fire extinguisher
Fire blanket
Eye washer
Chemical waste containers
126
Preventive measure
Read SOP or user’s manual before use
Always wear eye protective glasses
Consult the material safety data sheets for proper handling of chemicals
Use personal safety equipment when handling chemicals
Never conduct any experiments outside the fume hoods
Clean up spillage of chemicals immediately
Always store chemicals in the chemical storage locker
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 318: INSTRUMENT LABORATORY
Room responsible person:
Training responsible person:
Suthajini Yogarajah
Supervisor
ROOM ACTIVITIES


Research
Use of instruments
RULES TO FOLLOW

User must keep order and clean up after themselves.

Chemical spills should be removed immediately.

Sign in logbok, using instruments

Turn off lights when leaving room for the day.
Instruments and equipment
Name/type
2 pcs Freeze Drying
Christchurch 1-4
Metanol Bath
Vacuumpumpe for
evaporation
Responsible person
Training responsible
person
Comments
Suthajini Yogarajah
Suthajini Yogarajah
Suthajini Yogarajah
Water distilling apparatus
Suthajini Yogarajah
Lab. Dishwasher
Vacuum centrifuge
Suthajini Yogarajah
Anders Åsberg 4.etg.
RISK ANALYSIS
Risk
Preventive measure
Incorrect use of instruments and equipment
Read SOP or manual before use
Always check the flasks to find any cracks before
you use the freeze dryer. Always wear protective
goggles.
Cracks in the freeze dryer flasks may lead to
either explosion or implosion
Risk of water leakage
Closing the water supply to the water distilling
apparatus outside normal working hours.
Do not use the water distilling apparatus and
washing machine outside normal working
hours.
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY



Fire extinguisher
Fire blanket
Eye washer
127
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 330: KITCHEN/OFFICE
Room responsible person:
Training responsible person:
Engineer at the Pharmacognosy group
ROOM ACTIVITIES


Simple food making
Copying, scanning
RULES (KITCHEN)





Everyone is obliged to keep things in order in the kitchen.
If you store food in the kitchen, take care to remove it before it gets poor.
After making sandwiches or any other food, you have to tidy.
Dirty dishes have to be put in the dishwasher.
If there is a posting with the word “WASHED”, you may put the cleaned dishes in the kitchen
cupboard. If the diswasher is full of dirty dishes, you may add detergent and start the dishwasher. If
you don’t know how to do this, ask the room responsible person.
(There are some postings in Norwegian at the kitchen’s cupboard doors).
RULES (OFFICE)

INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Dishwasher
PC
Skanner
Printer
Ing.farmakognosi
Ellen Hanne Cohen
Ellen Hanne Cohen
Ellen Hanne Cohen
Training responsible
person
Comments
RISK ANALYSIS
Risk
Preventive measure
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

128
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 331: LABORATORY
Room responsible person:
Training responsible person:
Berit Smestad Paulsen
Supervisor
ROOM ACTIVITIES


Research
Use of instruments
RULES


The users must keep things in order and tidy.
Switch off the lights in the end of the day.
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Rotavapor
Plateleser
Suthajini Yogarajah
Suthajini Yogarajah
Training responsible
person
Comments
RISK ANALYSIS
Risk
Exposure to harmful chemicals
Accidents and injuries due to improper use of instruments and
equipment
Preventive measure
Clean up spillage of chemicals immediately
Always store chemicals in the chemical storage
locker
Read SOP or user’s manual before use
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY




Fire extinguisher
Fire blanket
Eye washer
Chemical waste containers
129
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 333: LABORATORY
Room responsible person:
Training responsible person:
Berit Smestad Paulsen
Supervisor
ROOM ACTIVITIES


Research
Use of instruments
RULES
 The users must keep things in order and tidy.
 Clean up spillage of chemicals immediately
 Switch off the lights in the end of the day.
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Fraction collector
Pipettes
Heating oven
Ellen Hanne Cohen
Ellen Hanne Cohen
Room responsible
person
Room responsible
person
Suthajini Yogarajah
Ultra sonic bath
Rotavapor
Training responsible
person
Comments
RISK ANALYSIS
Risk
Accidents and injuries due to improper use of instruments and
equipment
Preventive measure
Read SOP or user’s manual before use
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY




Fire extinguisher
Fire blanket
Eye washer
Chemical waste containers
130
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 334: INSTRUMENTLAB.
Room responsible person:
Training responsible person:
Berit Smestad Paulsen
Supervisor
ROOM ACTIVITIES


Research/lab courses
Use of instruments
RULES





The users must keep things in order and tidy up.
The users must remove paper and glass waste when it’s needed.
Fume cupboards that are not used shall have their hatches closed.
The lab scales must be cleaned after use.
Switch off the light in the end of the day.
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
GC Shimadzu
GC Carlo Erba
PC/Laser printer
HPLC Shimadzu
Radioactive detector
HPLC
Ellen H. Cohen
Training responsible
person
Comments
Ellen H. Cohen
Ellen H. Cohen
Ellen H. Cohen
RISK ANALYSIS
Risk
Accidents and injuries due to improper use of
instruments and equipment
Exposure to harmful chemicals
Leakage of combustible gases from gas cylinders.
Risk of explosion at a fire.
Preventive measure
Read SOP or user’s manual before use
Always wear protective glasses
Always store chemicals in the chemical storage locker
Clean up spillage of chemicals immediately
Refer to the Laboratory: Safety and the working environment
chapter for proper use and handling of pressureized gas
cylinders.
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY



Fire extinguisher
Fire blanket
Eye washer
131
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 336: DRUG COLLECTION
Room responsible person:
Training responsible person:
Permanent scientific employees at the department
Chief of lab courses/supervisors
ROOM ACTIVITIES

Student’s discussion groups

Drug exhibition
RULES

Always keep the room tidy.
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Training responsible
person
Comments
Blackboard
Large table
RISK ANALYSIS
Risk
Preventive measure
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY


Fire extinguisher
Fire blanket
132
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 339: STUDENTS COURSES’ LABORATORY
Room responsible person:
Suthajini Yogarajah
Training responsible person: Supervisor
ROOM ACTIVITIES


Students courses
Use of instruments
RULES TO FOLLOW
• User must keep order and clean up after themselves.
• Safty cabinets that are not in use shall be closed.
• The weights should be cleaned after each use.
• Turn off lights when leaving room for the day.
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Over weight
Analysis weight
UV-photo-meter
Suthajini Yogarajah
Centrifuge
Suthajini Yogarajah
pH-meter
Suthajini Yogarajah
Rotary evaporator
(organic solution)
Suthajini Yogarajah
Water bath
Chemical Cabinets
w/exhaust
Training responsible
person
Comments
Suthajini Yogarajah
Suthajini Yogarajah
Suthajini Yogarajah
RISK ANALYSIS
Risk
Preventive measure
Incorrect use of instruments and equipment
Read SOP or manual before use
Use safety data sheets for proper handling and
storage of each chemical
Exposure to hazardous chemicals
Personal protective equipment must be used when
handling chemicals.
Chemical spills should be removed immediately.
Chemicals should not be stored outside the
chemical cupboard / fridge.
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

Emergency shower(nearby)



Fire extinguisher

Eyewash bottles
Chemical Cabinets w / exhaust

Fire Blanket
First aid kit
133
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 339 B: CHROMATOGRAPHY LAB.
Room responsible person:
Training responsible person:
Suthajini Yogarajah
Supervisor
ROOM ACTIVITIES



Student Course
Research
Use of instruments
RULES TO FOLLOW





User must keep order and clean up after themselves.
Safety cabinets that are not in use shall be closed.
Chemicals should not be stored outside the chemical cupboard / fridge.
Chemical spills be removed immediately.
Turn off lights when leaving room for the day
INSTRUMENTS AND EQUIPMENT
Training
responsible person
Name/type
Responsible person
TLC tank
TLC spray Camag
Suthajini Yogarajah
UV equipment
Ultrasonic VWR
Suthajini Yogarajah
Suthajini Yogarajah
Heating cabinet Heraeus
Suthajini Yogarajah
DCC Chromatography
Buchi
Karl Malterud
Comments
Suthajini Yogarajah
RISK ANALYSIS
Risk
Preventive measure
Exposure to hazardous chemicals
Use safety data sheets for proper handling and
storage of each chemical
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY





134
Emergency shower(nearby)
Fire extinguisher
Fire Blanket
Eyewash bottles
Chemical Cabinets w / exhaust
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 340: PREPARATION ROOM/COURSES
Room responsible person:
Training responsible person:
Suthajini Yogarajah
Supervisor
ROOM ACTIVITIES

Preparation for students courses
RULES TO FOLLOW
• User must keep order and clean up after themselves .
• Safty cabinets that are not in use shall be closed.
• The weights should be cleaned after each use.
• Turn off lights when leaving room for the day.
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Over Weight
Pipettes
Suthajini Yogarajah
Training responsible
person
Comments
RISK ANALYSIS
Risk
Preventive measure
Exposure to hazardous chemicals
Use safety data sheets for proper handling and
storage of each chemical
Personal protective equipment must be used when
handling chemicals.
Chemicals should not be stored outside the
chemical cupboard / fridge.
Chemical spills should be removed immediately.
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY




Fire extinguisher
Fire Blanket
First aid kit
Eyewash bottles
135
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 344: LABORATORY
Room responsible person:
Training responsible person:
Ellen Hanne COhen
Supervisor
ROOM ACTIVITIES


Research/Lab courses
Use of instruments
RULES



The users must keep things in order and tidy up.
Fume cupboards that are not used shall have their hatches closed.
Switch off the light in the end of the day.
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
BioLC DIONEX
PC/Printer HP
Water purifying apparatus
Millipore
HPLC VARIAN
HOLGER
Ellen Hanne Cohen
Ellen Hanne Cohen
Ellen Hanne Cohen
PC/Printer HP
HPLC LaChrom VWR
Rotavapor
Versa Flash Supelco
Ellen Hanne Cohen
Helle Wangensteen
Suthajini Yogarajah
Helle Wangensteen
Training responsible
person
Comments
Helle Wangensteen
RISK ANALYSIS
Risk
Accidents and injuries due to improper
use of instruments and equipment
Exposure to harmful chemicals
Preventive measure
Read SOP or user’s manual before use
Always wear protective glasses
Always store chemicals in the chemical storage locker
Clean up spillage of chemicals immediately
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

Emergency shower (in the vicinity)

Fire extinguisher

Fire Blanket

Eyewash bottles

Chemical storage locker, ventilated

Chemical waste containers
136
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 358: BALANCE ROOM
Room responsible person:
Training responsible person:
Suthajini Yogarajah
ROOM ACTIVITIES

Weighing of samples
RULES TO FOLLOW

User must keep order and clean up after themselves.

The scale should be cleaned after each use.

Turn off lights when leaving room for the day.
Instruments and equipment
Name/type
Responsible person
Analytical scale Sartorius
Suthajini Yogarajah
Training responsible
person
Comments
RISK ANALYSIS
Risk
Preventive measure
Exposure of harmful toxic substances when
weighing
Wear protective equipment
Chemical spills should be removed
immediately.
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

Fire extinguisher
137
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 362: LABORATORY
Room responsible person:
Training responsible person:
Anne-Berit Samuelsen
Supervisor
ROOM ACTIVITIES


Research
Use of instruments
RULES



The users must keep things in order and tidy up.
Fume cupboards that are not used shall have their hatches closed.
Switch off the light in the end of the day.
INSTRUMENTS AND EQUIPMENT
Name/type
FPLC-AKTA
FPLC Pharmacia
RI-Detektor Shimadzu
BioRad Elektroforese
SDS-Page
Heating oven Hetosic
Smi-pipette
Rovapor
Heating block for evaporation
Phenol/sulfuric acid test
equipment
Responsible person
Kari Inngjerdigen/Ingvild
Austarheim
Anne-Berit Samuelsen
Ingvild/Tom-Erik
Ellen H. Cohen
Ellen H. Cohen
Training responsible person
Kari Inngjerdingen/Ingvild
Austarheim
Ellen H. Cohen
Ellen H. Cohen
Suthajini Yogarajah
RISK ANALYSIS
Risk
Accidents and injuries due to improper use of
instruments and equipment
Exposure to harmful chemicals
Preventive measure
Read SOP or user’s manual before use
Always wear protective glasses
Always store chemicals in the chemical storage locker
Clean up spillage of chemicals immediately
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

Fire extinguisher

Fire Blanket

Eyewash bottles

Chemical waste containers
138
Comments
School of Pharmacy HSE Manual: Department of Pharmaceutical Chemistry
BL25 365: CONFERENCE ROOM
Room responsible person: : Ellen Hanne Cohen
Training responsible person:
ROOM ACTIVITIES



Conference room
Common room for the Department
Library room for the Department
RULES


The users must keep things in order and tidy up.
Switch off the light in the end of the day.
INSTRUMENTS AND EQUIPMENT
Name/type
Responsible person
Common PC
Overhead projector
Wall screen
Ellen H. Cohen
Ellen H. Cohen
Ellen H. Cohen
Training responsible
person
Comments
RISK ANALYSIS
Risk
Preventive measure
INSTALLATIONS REGARDING HEALTH, ENVIRONMENT AND SECURITY

The room nearby
139
School of Pharmacy HSE Manual: Appendices
APPENDICES
PRODUCT LABELLING OF CHEMICALS
HAZARD SYMBOLS WITH HAZARD DESIGNATION
The following hazard codes, hazard symbols and hazard designation apply at labelling of chemicals.
E
O
F+
F+
C
EXPLOSIVE
OXIDISING
T+
VERY TOXIC
EXTREMELY FLAMMABLE
T
TOXIC
N
HIGHLY FLAMMABLE
Xn
DANGEROUS
FOR THE
ENVIRONMENT
HARMFUL
CORROSIVE
Xi
IRRITANT
The hazard symbols must be printed in black on an orange background. The hazard designation must be
denoted together with the hazard symbol. The hazard code that is above the symbols (e.g. F, Xi) is not to be
included on the hazard label.
HAZARD CLASSES BASED ON RISK PHRASES
Hazard class - § 3 and
§6
Explosive
Oxidising
Extremely flammable
Risk phrases
Hazard symbol
R2, R3
R7, R8, R9
R12
Hazard
code
E
O
F+
Highly flammable
Flammable
Very toxic
R11, R15, R17
R10
R26, R27, R28, R39
F+
None
T+
Toxic
R23, R24, R25, R39, R48
T
Harmful
Corrosive
R20, R21, R22, R68, R48, R65
R34, R35
Xn
C
Irritant
Sensitiser
R36, R37, R38, R41
R42
Xi
Xn
Flame symbol
None
Skull and
crossbones
Skull and
crossbones
X-cross
Corrosive
pictogram
X-cross
X-cross
140
Explosive
Oxidising
Flame symbol
Hazard
designation
Explosive
Oxidising
Extremely
flammable
Highly flammable
None
Very toxic
Toxic
Harmful
Corrosive
Irritant
Harmful
School of Pharmacy HSE Manual: Appendices
Carcinogenic
R43
R45, R49
Xi
T
Mutagenic
R40
R46
Xn
T
R68
R60, R61
Xn
T
R62, R63
R50, R50/53, R51/53, R54, R55,
R56, R57, R58, R59
Xn
N
R52, R52/53, R53
None
Harmful to
reproduction
Dangerous for the
environment
X-cross
Skull and
crossbones
X-cross
Skull and
crossbones
X-cross
Skull and
crossbones
X-cross
Dangerous for the
environment
pictogram
None
Irritant
Toxic
Harmful
Toxic
Harmful
Toxic
Harmful
Dangerous for the
environment
None
141
School of Pharmacy HSE Manual: Appendices
HAZARD CLASSES
CRITERIA FOR HSE ASSESSMENT OF CHEMICALS
Colour codes:
Within the limit
Acceptable
Akseptabel
Factor
assessed
HSE
datasheet
section
Not
acceptable
Uakseptabel
HAZARD CLASS
Lowest hazard class
Highest hazard class
1
2
3
4
5
T = Toxic
T+ = Very toxic
R42
F+ = Extremely
flammable
O = Oxidising
E = Explosive
Health
hazard
15
NO=Label
not
mandatory
NO=Label
not
mandatory
Xi = Irritant
Xn = Harmful
C = Corrosive
R43
Fire hazard
15
NO=Label
not
mandatory
NO=Label
not
mandatory
R10,
R5, R18,
R30, R44
F = Highly
flammable
YL-group
15
00-0
1
2-3
4-5
R52, R53
N = Dangerous
for the
environment R59
N = Dangerous
for the
environment
R50
External
environment
15
(YL: Occupational Health and Hygiene ventilation requirements)
GAS CYLINDERS COLOUR CODES
UiO’s gas cylinder supplier AGA has introduced shoulder marking on the gas cylinders in accordance with the
new European standard EN 1089-3. The shoulder marking only provides information about the gasses’
properties in air, but most pure gasses have been allocated a dedicated colour. More detailed information on
the gas cylinder content is to be found on the product label.
SHOULDER COLOUR (EN 1089-3: GENERAL )
Toxic and/or corrosive gasses
Yellow
Flammable gasses
Red
Oxidising gasses
Light blue
Inert gasses
Bright green
SHOULDER COLOUR (EN 1089-3: PURE GASSES)
Acetylene
Maroon
Carbon dioxide
Grey
Oxygen
White
Helium
Brown
Argon
Dark green
Hydrogen
Red
Nitrogen
Black
Nitrous oxide
Blue
CYLINDER COLOURS (AGA GAS SUPPLIER)
Industrial gasses
Black
Acetylene
Maroon
Food industry gasses
Green
Special gasses
Silver-grey
Medical gasses
White
142
School of Pharmacy HSE Manual: Appendices
RISK PHRASES
At hazard marking of dangerous chemicals, the following statements (R phrases) must be used to describe the
hazards:
R1
R2
R3
R4
R5
R6
R7
R8
R9
R10
R11
R12
R14
R15
R16
R17
R18
R19
R20
R21
R22
R23
R24
R25
R26
R27
R28
R29
R30
R31
R32
R33
R34
R35
R36
R37
R38
R39
R40
R41
R42
R43
R44
R45
R46
R48
R49
R50
R51
R52
R53
R54
R55
R56
R57
R58
R59
R60
R61
R62
R63
R64
R65
R66
R67
R68
Explosive when dry
Risk of explosion by shock, friction, fire or other sources of ignition
Extreme risk of explosion by shock, friction, fire or other sources of ignition
Forms very sensitive explosive metallic compounds
Heating may cause an explosion
Explosive with or without contact with air
May cause fire
Contact with combustible material may cause fire
Explosive when mixed with combustible material
Flammable
Highly flammable
Extremely flammable
Reacts violently with water
Contact with water liberates extremely flammable gases
Explosive when mixed with oxidising substances
Spontaneously flammable in air
In use, may form flammable/explosive vapour-air mixture
May form explosive peroxides
Harmful by inhalation
Harmful in contact with skin
Harmful if swallowed
Toxic by inhalation
Toxic in contact with skin
Toxic if swallowed
Very toxic by inhalation
Very toxic in contact with skin
Very toxic if swallowed
Contact with water liberates toxic gas
Can become highly flammable in use
Contact with acids liberates toxic gas
Contact with acids liberates very toxic gas
Danger of cumulative effects
Causes burns
Causes severe burns
Irritating to eyes
Irritating to respiratory system
Irritating to skin
Danger of very serious irreversible effects
Limited evidence of a carcinogenic effect
Risk of serious damage to eyes
May cause sensitisation by inhalation
May cause sensitisation by skin contact
Risk of explosion if heated under confinement
May cause cancer
May cause heritable genetic damage
Danger of serious damage to health by prolonged exposure
May cause cancer by inhalation
Very toxic to aquatic organisms
Toxic to aquatic organisms
Harmful to aquatic organisms
May cause long-term adverse effects in the aquatic environment
Toxic to flora
Toxic to fauna
Toxic to soil organisms
Toxic to bees
May cause long-term adverse effects in the environment
Dangerous for the ozone layer
May impair fertility
May cause harm to the unborn child
Possible risk of impaired fertility
Possible risk of harm to the unborn child
May cause harm to breast-fed babies
Harmful: may cause lung damage if swallowed
Repeated exposure may cause skin dryness or cracking
Vapours may cause drowsiness and dizziness
Possible risk of irreversible effects
143
School of Pharmacy HSE Manual: Appendices
RISK PHRASES IN COMBINATION
When several phrases are combined in one sentence, the phrases must be as specified below. The combination
is considered to be one sentence:
R14/15
R15/29
R20/21
R20/22
R20/21/22
R21/22
R23/24
R23/25
R23/24/25
R24/25
R26/27
R26/28
R26/27/28
R27/28
R36/37
R36/38
R36/37/38
R37/38
R39/23
R39/24
R39/25
R39/23/24
R39/23/25
R39/24/25
R39/23/24/25
R39/26
R39/27
R39/28
R39/26/27
R39/26/28
R39/27/28
R39/26/27/28
R42/43
R48/20
R48/21
R48/22
R48/20/21
R48/20/22
R48/21/22
R48/20/21/22
swallowed
R48/23
R48/24
R48/25
R48/23/24
R48/23/25
R48/24/25
R48/23/24/25
swallowed
R50/53
R51/53
R52/53
R68/20
R68/21
R68/22
R68/20/21
R68/20/22
R68/21/22
R68/20/21/22
144
Reacts violently with water, liberating extremely flammable gasses
Contact with water liberates toxic, extremely flammable gases
Harmful by inhalation and in contact with skin
Harmful by inhalation and if swallowed
Harmful by inhalation, in contact with skin and if swallowed
Harmful in contact with skin and if swallowed
Toxic by inhalation and in contact with skin
Toxic by inhalation and if swallowed
Toxic by inhalation, in contact with skin and if swallowed
Toxic in contact with skin and if swallowed
Very toxic by inhalation and in contact with skin
Very toxic by inhalation and if swallowed
Very toxic by inhalation, in contact with skin and if swallowed
Very toxic in contact with skin and if swallowed
Irritating to eyes and respiratory system
Irritating to eyes and skin
Irritating to eyes, respiratory system and skin
Irritating to respiratory system and skin
Toxic: danger of very serious irreversible effects through inhalation
Toxic: danger of very serious irreversible effects in contact with skin
Toxic: danger of very serious irreversible effects if swallowed
Toxic: danger of very serious irreversible effects through inhalation and in contact with skin
Toxic: danger of very serious irreversible effects through inhalation and if swallowed
Toxic: danger of very serious irreversible effects in contact with skin and if swallowed
Toxic: danger of very serious irreversible effects through inhalation, in contact with skin and if swallowed
Very toxic: danger of very serious irreversible effects through inhalation
Very toxic: danger of very serious irreversible effects in contact with skin
Very toxic: danger of very serious irreversible effects if swallowed
Very toxic: danger of very serious irreversible effects through inhalation and in contact with skin
Very toxic: danger of very serious irreversible effects through inhalation and if swallowed
Very toxic: danger of very serious irreversible effects in contact with skin and if swallowed
Very toxic: danger of very serious irreversible effects through inhalation, in contact with skin and if swallowed
May cause sensitisation by inhalation and skin contact
Harmful: danger of serious damage to health by prolonged exposure through inhalation
Harmful: danger of serious damage to health by prolonged exposure in contact with skin
Harmful: danger of serious damage to health by prolonged exposure if swallowed
Harmful: danger of serious damage to health by prolonged exposure through inhalation and in contact with skin
Harmful: danger of serious damage to health by prolonged exposure through inhalation and if swallowed
Harmful: danger of serious damage to health by prolonged exposure in contact with skin and if swallowed
Harmful: danger of serious damage to health by prolonged exposure through inhalation, in contact with skin and if
Toxic: danger of serious damage to health by prolonged exposure through inhalation
Toxic: danger of serious damage to health by prolonged exposure in contact with skin
Toxic: danger of serious damage to health by prolonged exposure if swallowed
Toxic: danger of serious damage to health by prolonged exposure through inhalation and in contact with skin
Toxic: danger of serious damage to health by prolonged exposure through inhalation and if swallowed
Toxic: danger of serious damage to health by prolonged exposure in contact with skin and if swallowed
Toxic: danger of serious damage to health by prolonged exposure through inhalation, in contact with skin and if
Very toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment
Toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment
Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment
Harmful: possible risk of irreversible effects through inhalation
Harmful: possible risk of irreversible effects in contact with skin
Harmful: possible risk of irreversible effects if swallowed
Harmful: possible risk of irreversible effects through inhalation and in contact with skin
Harmful: possible risk of irreversible effects through inhalation and if swallowed
Harmful: possible risk of irreversible effects in contact with skin and if swallowed
Harmful: possible risk of irreversible effects through inhalation, in contact with skin and if swallowed
School of Pharmacy HSE Manual: Appendices
SAFETY PHRASES
At hazard marking, the following phrases must be used to describe necessary preventive measures for safe
handling and use of the chemicals:
S1
S2
S3
S4
S5
S6
S7
S8
S9
S12
S13
S14
S15
S16
S17
S18
S20
S21
S22
S23
S24
S25
S26
S27
S28
S29
S30
S33
S35
S36
S37
S38
S39
S40
distributor)
S41
S42
S43
S45
S46
S47
S48
S49
S50
S51
S52
S53
S56
S57
S59
S60
S61
S62
S63
S64
S210
Keep locked up
Keep locked up and out of the reach of children
Keep in a cool place
Keep away from living quarters
Keep contents under ... (appropriate liquid to be specified by the manufacturer, importer or distributor)
Keep contents under ... (inert gas to be specified by the manufacturer, importer or distributor)
Keep container tightly closed
Keep container dry
Keep container in a well-ventilated place
Do not keep the container sealed
Keep away from food, drink and animal feeding stuffs
Keep away from ... (incompatible materials to be indicated by the manufacturer, importer or distributor)
Keep away from heat
Keep away from sources of ignition - No smoking
Keep away from combustible material
Handle and open container with care
When using do not eat or drink
When using do not smoke
Do not breathe dust
Do not breathe gas/fumes/vapour/spray (appropriate wording to be specified by
the manufacturer, importer or distributor)
Avoid contact with skin
Avoid contact with eyes
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice
Take off immediately all contaminated clothing
After contact with skin, wash immediately with plenty of ... (to be specified by the manufacturer, importer or
distributor)
Do not empty into drains
Never add water to this product
Take precautionary measures against static discharges
This material and its container must be disposed of in a safe way.
Wear suitable protective clothing
Wear suitable protective gloves
In case of insufficient ventilation wear suitable respiratory equipment
Wear eye/face protection
To clean the floor and all objects contaminated by this material use ... (to be specified by the manufacturer, importer or
In case of fire and/or explosion do not breathe fumes
During fumigation/spraying wear suitable respiratory equipment (appropriate wording to be specified by
the manufacturer, importer or distributor)
In case of fire use ... (the manufacture, importer or distributor must indicate in the space the precise type of
fire-fighting equipment. If water increases the risk add - Never use water)
In case of accident or if you feel unwell seek medical advice immediately (show the label where possible)
If swallowed, seek medical advice immediately and show this container or label
Keep at temperature not exceeding ... ° C (to be specified by the manufacturer, importer or
distributor)
Keep wet with ... (appropriate material to be specified by the manufacturer, importer or distributor)
Keep only in the original container
Do not mix with ... (to be specified by the manufacturer, importer or distributor)
Use only in well-ventilated areas
Not recommended for interior use on large surface areas
Avoid exposure - obtain special instructions before use
Dispose of this material and its container at hazardous or special waste collection point
Use appropriate containment to avoid environmental contamination
(Ensure safe packaging to prevent environmental contamination).
Refer to manufacturer/importer or distributor for information on recovery/recycling
This material and its container must be disposed of as hazardous waste
Avoid release to the environment. Refer to special instructions/HSE safety data sheet for further
information
If swallowed, do not induce vomiting: seek medical advice immediately and show this container or
label
In case of accident by inhalation: remove casualty to fresh air and keep at rest
If swallowed, rinse mouth with water (only if the person is conscious)
Use compressed air or fresh air line breathing apparatus in confined spaces
145
School of Pharmacy HSE Manual: Appendices
SAFETY PHRASES IN COMBINATION
When several phrases are combined in one sentence, the phrases must be as specified below. The combination
is considered to be one sentence:
S1/2
S3/7
S3/9/14
S3/9/14/49
S3/9/49
S3/14
distributor)
S7/8
S7/9
S7/47
S20/21
S24/25
S27/28
S29/35
S29/56
S36/37
S36/37/39
S36/39
S37/39
S47/49
146
Keep locked up and out of the reach of children
Keep container tightly closed in a cool place
Keep in a cool, well-ventilated place away from …
(to be specified by the manufacturer, importer or distributor)
Keep only in the original container in a cool, well-ventilated place away from ...
(incompatible materials to be indicated by the manufacturer, importer or distributor)
Keep only in the original container in a cool, well-ventilated place
Keep in a cool place away from ... (incompatible materials to be indicated by the manufacturer, importer or
Keep container tightly closed and dry
Keep container tightly closed and in a well-ventilated place
Keep container tightly closed and at temperature not exceeding ... ° C.
(to be specified by the manufacturer, importer or distributor)
When using do not eat or drink or smoke
Avoid contact with skin and eyes
After contact with skin, take off immediately all contaminated clothing, and wash immediately with plenty of ...
(to be specified by the manufacturer, importer or distributor)
Do not empty into drains; dispose of this material and its container in a safe way
Do not empty into drains, dispose of this material and its container
at hazardous or special waste collection point
Wear suitable protective clothing and gloves
Wear suitable protective clothing, gloves and eye/face protection
Wear suitable protective clothing and eye/face protection
Wear suitable gloves and eye/face protection
Keep only in the original container at temperature not exceeding ... ° C.
(to be specified by the manufacturer, importer or distributor)
School of Pharmacy HSE Manual: Appendices
ROUTINE TEMPLATE FOR ROOM - BL2X XXX: XXXXX
(The routine was last updated on 11.05.2010)
Main/Room responsible: xxxxx
Training responsible:
xxxxx
ACTIVITIES

xxxxx
RULES

xxxxx
INSTRUMENTS/EQUIPMENT
Name/type
Responsible for
Instruments
Responsible for
Training
Comments
Xxxx
RISK ASSESSMENT
Risk
Preventive measure
HSE INSTALLATIONS

xxxxx
147
LIST OF TELEPHONE NUMBERS AND ADDRESSES
UiO (Blindern)
Switchboard: 9/ 22 85 50 50
POLICE
112
AMBULANCE
113
FIRE
110
Name
Fire Services emergency
no.
Fire safety at UiO:
Fire safety advisor
Address
Tel. no.:
E-mail/Web page
110
Technical
Department
PO 1077
Blindern
22 85 67 25
[email protected]
http://www.uio.no/foransatte/drift/sikkerhet/brannvern/
Ambulance emergency no.
113
Oslo A&E
Ullevål University
Hospital
22 93 22 93
02770
22 11 80 80
22 11 73 50
http://www.oslolegevakt.no/
Health Services, UiO
Emergency help
Norwegian Poison
Information Centre
Taxi – Emergency help
Police emergency no.
22 85 31 74
http://www.sio.no/wps/portal/sio/hels
e?reset=true
http://www.giftinfo.no/
Security and alarm centre,
UiO
EMERGENCY tel.
22 85 66 66
Security and alarm centre,
UiO
Non-emergency messages.
22 85 50 07
Switchboard
Emergency
reception
Ophthal22 11 85 45
mology Dept. 22 11 85 47
22 59 13 00
22 38 80 50
112
[email protected]
https://www.uio.no/foransatte/drift/sikkerhet/beredskapsplan/
School of Pharmacy:
Director, School of
Pharmacy
PO 1068
Blindern
22 85 65 86
School of Pharmacy:
Reception
PO 1068
Blindern
22 85 65 85
VIP 31494
[email protected]
http://www.farmasi.uio.no
Technical Dept. Lower
Blindern:
Operations office
22 85 72 93
[email protected]
http://www.admin.uio.no/ta
Department of Chemistry:
Head of Safety
22 85 54 69
91 33 71 73
VIP 31154
22 85 54 46
[email protected]
Section for HSE
at UiO
22 85 88 95
Labour Inspection
Authority
2nd District
23 08 05 05
[email protected]
http://www.uio.no/om/organisasjon/ad
m/opa/hms/
http://www.arbeidstilsynet.no/
Department of Chemistry:
Reception
[email protected]
http://www.kjemi.uio.no
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