AB2 Service Manual

AB2 Service Manual
AMINCO-Bowman™ Series 2
Luminescence
Spectrofluorometer
Service Manual
Note
This manual is being revised, so some of the
information you will find in it is out-of-date. Please
accept our apologies for any confusion this may cause.
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Corporation, and the contact and trademark information
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P/N AB2-10020
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NOTE
This manual contains information, instructions and specifications for the Nicolet
AMINCO-Bowman Series 2 Luminescence (AB2) spectrometer that were believed accurate at the
time this manual was written. However, as part of Thermo Electron Corporation’s on-going
program of product development, the specifications and operating instructions may be modified or
changed from time to time. Thermo Electron Corporation reserves the right to change such
operating instructions and specifications. Under no circumstances shall Thermo Electron
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instructions or specifications relating to Thermo Electron Corporation products, nor shall Thermo
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NEW PRODUCT WARRANTY
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This warranty covers parts (except those specified below) and labor, and applies only to
equipment which has been installed and operated in accordance with the operator's reference
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ii
Table of Contents
System Description and Overview................................................................................................... 1
Overview
............................................................................................................................ 1
System description....................................................................................................................... 1
Installation Procedures .................................................................................................................... 3
Preinstallation checks .................................................................................................................. 3
GPIB board installation ................................................................................................................ 3
Prepare the computer for software installation ............................................................................ 3
Installing the AB2 software .......................................................................................................... 3
Installing the GPIB device driver software ................................................................................... 3
Installing the AB2 ......................................................................................................................... 3
Interface the instrument to the computer ................................................................................. 4
Checking the instrument for proper operation.......................................................................... 5
Perform Signal-to-Noise ratio test................................................................................................ 7
Complete documentation ......................................................................................................... 8
Demonstrate instrument operation........................................................................................... 8
System Description........................................................................................................................ 10
Overview
.......................................................................................................................... 10
Instrument system components ................................................................................................. 10
Light source ............................................................................................................................ 10
Monochromators .................................................................................................................... 10
Sample compartment ............................................................................................................. 11
Filter/lens holders ................................................................................................................... 11
Circuit Description for the High Voltage and Monochromator Driver Board.................................. 11
Overview
.......................................................................................................................... 11
High voltage
.......................................................................................................................... 11
Slit motor control ........................................................................................................................ 11
Grating motor control ................................................................................................................. 12
Solenoid control ......................................................................................................................... 13
Filter/Polarizer Wheels Driver Board ............................................................................................. 13
Circuit description ...................................................................................................................... 13
PMT ........................................................................................................................................ 13
Monochromators .................................................................................................................... 14
Bandpass................................................................................................................................ 14
Computer................................................................................................................................ 14
Printer and plotter................................................................................................................... 14
Accessories ............................................................................................................................ 14
Overview of power up diagnostic functions ............................................................................... 15
CPU/Analog Board Circuit Descriptions ........................................................................................ 15
Lamp On/Power Delay Board........................................................................................................ 20
Circuit description ...................................................................................................................... 20
Lamp on.................................................................................................................................. 20
Power delay............................................................................................................................ 20
Hour meter.............................................................................................................................. 20
Lamp monitor.......................................................................................................................... 20
Troubleshooting Guide .................................................................................................................. 27
Hardware Maintenance Procedures .............................................................................................. 30
Procedure 1. Removal and Replacement of the Top Cover .................................................. 30
Procedure 2. Monochromator maintenance procedures........................................................ 32
Checking slit calibration.......................................................................................................... 32
Removal and replacement of monochromators ..................................................................... 32
Removal and replacement of excitation monochromator....................................................... 32
Removal and replacement of emission monochromator........................................................ 35
Check monochromator calibration ......................................................................................... 37
Procedure 3. Removal and replacement of circuit boards ..................................................... 47
iii
Procedure 4.
Installing the Lens Spacer between the Emission Monochromator and the
Sample Holder .................................................................................................. 47
Procedure 5. Entering calibration values on the CPU board ................................................. 48
Procedure 6. Installing the GPIB cable .................................................................................. 49
Procedure 7. Removal and Replacement of the Photomultiplier Tube FA-277 (R2949) ....... 49
Optimize the PMT output signal ............................................................................................. 50
Procedure 8. Installing Filter Wheels ..................................................................................... 51
Emission Filter Wheel installation: ......................................................................................... 51
Excitation Filter Wheel installation: ........................................................................................ 52
Checking Filter Wheel operation: ........................................................................................... 52
Procedure 9. Checking Filter Holder placement and optical alignment ................................. 53
Filter Holder placement: ......................................................................................................... 53
Aligning the beam focusing mirror:......................................................................................... 53
Procedure 10. Removal and replacement of the Motherboard Assembly ............................... 55
Procedure 11. Removal and Replacement of the Power Supply............................................. 55
Power supply removal: ........................................................................................................... 55
Adjust CW Lamp Current: ...................................................................................................... 56
Procedure 12. Removal and replacement of the CW lamp...................................................... 57
Cartridge Assembly ................................................................................................................ 57
Lamp Housing Assembly........................................................................................................ 59
Procedure 13. CW Lamp hard start ......................................................................................... 61
Step 1:
Replacing a faulty lamp .................................................................................... 62
Step 2:
Adjusting the ILC lamp power supply ............................................................... 62
Step 3:
Replacing the ILC power supply....................................................................... 63
Step 4:
Replacing the CW lamp power supply cables .................................................. 63
Step 5:
Check for faulty electrical connections ............................................................. 64
Step 6:
Perform CW lamp housing assembly upgrade................................................. 64
Procedure 14. PMT cooling upgrade ....................................................................................... 65
Upgrade Kit Components: ...................................................................................................... 65
Preinstallation System Checkout:........................................................................................... 66
System Upgrade Procedure: .................................................................................................. 67
System checkout and PMT adjustment.................................................................................. 68
Parts List........................................................................................................................................ 70
INSTALLATION REPORT ............................................................................................................. 91
iv
v
System Description and Overview
Overview
The Nicolet AMINCO-Bowman Series 2 Luminescence Spectrometer (AB2) is a high
performance, low-cost spectrofluorometer. It features holographic grating excitation and emission
monochromators, which can perform scans from 3 to 6000nm per minute. The monochromators
have a slew rate of 12000nm per minute with a resolution of 0.2nm per step. The bandpass for
each monochromator can be set from 0.5 to 16nm. The instrument has an effective range of
operation from 220nm to 850nm, and a Signal-to-Noise ratio greater than 900 to 1 with the CW
lamp.
The instrument weighs 112 lbs. and only requires a 17.25” x 31” x 9” area of bench space. The
instrument has an efficient modular design, which simplifies instrument service. It also has
excellent interface capabilities between the instrument and the controlling computer.
The computer software uses graphic user interface to control most of the instrument’s functions.
The multitasking windowing environment of the software gives the extra-added ability to perform
multiple computer tasks at once. The system also incorporates a wide range of accessories that
can be used to apply the instrument to many experimental needs.
The instrument can be configured to use a continuous wave lamp, a Flash Lamp, or both as an
excitation source. It can also be equipped with a set of film polarizer/Filter Wheels to do
polarization measurements and varying bandpass operations. The AB2 is designed to be a userfriendly instrument. It can be used to free up valuable lab time. It provides the user with a
powerful analytical tool.
System description
Refer to the System Block Diagram for an overall system description. The instrument is made up
of the following major subassemblies:
Power supply module (PN: SQ-390)
Continuous wave xenon lamp (PN: SQ-310)
Flash Lamp (optional) (PN: SQ-311)
Lamp change mirror (used when Flash Lamp is installed) (PN: SQ-312
Electronics card cage (PN: SQ-326)
Excitation monochromator (PN: SQ-301)
Excitation polarization/Filter Wheel (optional) (PN: SQ-320)
Focusing mirror assembly
Beam splitter and photodiode assembly
Sample chamber
Emission polarization/Filter Wheel (optional) (PN: FA-263)
Emission monochromator (PN: SQ-301)
Photomultiplier tube (PMT) (PN: FA-277)
The power supply module generates the power necessary for electrical needs of the instrument. It
supplies power to the continuous wave xenon lamp, the Flash Lamp (if installed); the electronics
card cage, and cooling fans of the unit.
The continuous wave lamp supplies a 150-watt source of light for excitation, emission and kinetic
measurements.
The Flash Lamp, when installed, is used to develop a flashing light source, which enables the
user to measure rate of decay of emission signals.
1
The lamp change mirror is installed in the instrument when both lamp sources are used. It
provides a means for selecting the continuous wave lamp or the Flash Lamp as the light source.
The electronics card cage houses a motherboard assembly that distributes power from the power
supply to the other components of the instrument. It also provides the backplane for plugging the
CPU/Analog, High Voltage/Mono, and polarization/Filter Wheel boards into the instrument.
Signals generated on each of these boards are distributed throughout the instrument by way of
connector cables, which are attached to the motherboard.
The excitation monochromator contains a ruled holographic grating, which separates the white
light from the light source into individual wavelengths of light. The individual wavelengths are
seen as a spectrum of colors. The spectrum of light is then routed through the sample to excite
the cells and molecules into higher states of energy. Housed inside the excitation monochromator
is a slit assembly to control the amount of light allowed to pass through the monochromator.
There is also a motor attached to the mono grating to provide a means for selecting an individual
wavelength of light, scanning through selected portions of the spectrum, or for scanning the entire
spectrum. A shutter assembly is also attached to the monochromator to isolate the sample from
the light source.
The excitation polarization/Filter Wheel assembly contains three polarizer settings (0, 35.3 and 90
degrees) for performing polarization measurements. There are also two filter settings (370nm and
630nm) for doing interference of bandpass measurements and an open position.
The focusing mirror assembly is used to focus the light beam, and to ensure that the beam
follows the proper path through the beam splitter and sample chamber.
The beam splitter and photodiode assembly performs two functions. First, it separates
approximately 10% of the excitation light source from the light beam. This small amount of light is
reflected back into the photodiode. Secondly, the photodiode uses the light signal from the beam
splitter as a reference signal to correct for fluctuations that occur in the xenon lamp output.
The sample chamber provides a means of placing a solution in the light path for experimentation.
The sample chamber is manufactured such that different accessories can be installed to
accommodate changing experimental needs. There are also two filter holder assemblies mounted
inside the sample chamber that can be used to install bandpass/interference filters in the light
path. These filter holder assemblies also house focusing lenses to ensure proper light beam
trajectory into and out of the sample chamber.
The emission polarization/Filter Wheel is identical in construction and operation to that of the
excitation polarization/Filter Wheel assembly except for a 54.7-degree angle polarizer in place of
the 35.3.
The emission monochromator is also identical in construction and operation of the excitation
monochromator. The major difference in its operation is the fact that it is used to measure the
amount of fluorescence or luminescence given off from the experimental sample.
The photomultiplier tube (PMT) is used to collect the light energy that is passed through the
emission monochromator, and to convert the light energy into an electrical current. The PMT
current is routed back to the CPU/Analog board for conversion into a data signal that is ultimately
displayed or stored for the user.
2
Installation Procedures
Preinstallation checks
1. Verify that adequate table space is available for the instrument, computer and printer.
2.
Verify that there are adequate power outlets for the instrument. A power strip with surge
protection and signal filtering is recommended. Power outlets should exist for the following
components:
a.
The AB2 (one required).
b.
The computer (one required).
c.
The video monitor (one required).
d.
The printer or plotter (one required if printer/plotter is available).
3.
As you unpack the boxes, arrange all components in a neat orderly fashion. Verify that all
components listed on the invoice have been received with the shipment. Also check the
condition of the components to see if any damage has occurred during the shipment.
GPIB board installation
Install the GPIB interface board in the computer according the directions that came with the
board, or in the Installation Guide section of the operator’s manual.
Prepare the computer for software installation
1. Connect the power cord to the computer and monitor.
2.
Connect the keyboard to the computer.
3.
Connect the monitor to the computer.
4.
Connect the mouse to the computer.
5.
Turn the power on to the computer and monitor.
NOTE: It is assumed that the computer already has a working version of Windows installed.
Installing the AB2 software
Install the AB2 software according the directions in the Installation Guide section of the operator’s
manual.
Installing the GPIB device driver software
Install the GPIB device driver software according the directions in the Installation Guide section of
the Operator’s manual.
Installing the AB2
CAUTION:
LIFTING THE INSTRUMENT FROM ITS SHIPPING CONTAINER AND TO
THE LAB BENCH IS A TWO-PERSON OPERATION. BE SURE TO KEEP
YOUR BACK STRAIGHT, KEEP THE INSTRUMENT AS CLOSE TO YOUR
BODY AS POSSIBLE, AND LIFT WITH YOUR LEGS. IMPROPER LIFTING
CAN RESULT IN BODILY INJURY.
1.
Remove the instrument from its shipping carton and set it on the lab table.
2.
Remove the top cover from the unit.
3
3.
Perform a visual inspection of all assemblies to ensure that no damage has occurred during
shipment.
4.
Check the address jumpers of the upper left corner of the CPU board to ensure that they are
set for address #1.
Check the calibration numbers on the rotary switches located on the upper left corner of the CPU
board. Crosscheck these values with the values on the instrument’s monochromators. Record
these values.
5.
Remove the tie-downs from the shutters and the lamp change mirror if so equipped.
6.
Connect the power cord to the left end of the instrument.
7.
Ensure that the lamp enable switch is in the ON position.
8.
Power up the instrument. Ensure that the following conditions occur:
a.
The CW lamp ignites.
b.
The lamp monitor LED on the front of the instrument glows red.
c.
The LED on the right side of the instrument changes from yellow to green.
9.
Shut off the power to the unit. Wait approximately 15 minutes before reigniting the lamp.
Interface the instrument to the computer
1. Connect the GPIB interface cable between the computer and the instrument.
2.
Connect the printer/plotter (if available) to the computer.
3.
Turn on power to the instrument.
4.
Turn on power to the computer and monitor. The screen should show the AB2 icon on the
Desktop.
5.
Turn on the printer/plotter.
6.
Start the AB2 software by selecting its icon The following conditions should occur:
a.
The computer screen should display the AB2 software window.
b.
The message “Initializing instrument . . .” should appear in the lower left side of the
window.
c.
You should hear mechanical sounds from the instrument as it goes through its
initialization and calibration routines.
d.
The message on the screen will disappear after the initialization process is
completed.
7.
Select Setup from the main menu window.
8.
Select instrument from the Setup menu
9.
Select monochromators from the Instrument menu.
10. Click the mouse on the excitation Open Shutter selection, and listen to the instrument to
verify that the shutter opens. Perform the same function for the emission monochromator.
Exercise both shutters several times to ensure that they operate smoothly. If either shutter fails to
open or close, or if they jam during operation, lubricate the faulty shutter with liquid graphite.
4
11. Using an acid brush, “paint” a thin coat of Slip Plate graphite based lubricant to the shutters
[SQ-228] or [SQ-240] and to the sticking shutter arm (OPN710) in the areas shown below:
SQ-228 & SQ-240
Apply along both
edges and overlap
OPN-710
Apply on inside surface. Do
not overlap
Figure 1 Shutter Lubrication Points
12. Let dry one (1) hour then gently scrape off excess lubricant.
Checking the instrument for proper operation
Required equipment:
For this procedure, a clean quarts cuvette of pure distilled water will be needed. It is extremely
important that the cuvette is thoroughly clean and the distilled water is as free of contaminants as
possible. The factory get their water from the local grocery store, but never keeps it for more than
a month.
1. Place a clean quartz cuvette of distilled water in the sample chamber.
2.
Select Setup from the menu bar.
3.
Choose Instrument Setup from the drop down menu.
4.
Choose Monochromators from the Setup window.
5.
Ensure that the excitation monochromator is set to 350nm.
6.
Ensure that the emission monochromator is set to 397nm.
7.
Set the emission step size to 0.2nm.
8.
Set both bandpass settings to 4nm.
9.
Open both shutters.
10. Close the Monochromator window by clicking on the OK button.
11. Choose Instrument Setup from the drop down menu.
12. Select Sensitivity from the Setup window.
5
13. Select Auto-range and wait for the software to set the high PMT voltage.
NOTE:
At this point, there should be a reading of approximately 60% on the EM channel.
The actual amount of high voltage that must be applied to achieve this signal
level may vary, however, if it exceeds 1000 volts, there is probably an error in the
instrument. If this condition occurs, refer to the optics alignment section of this
manual.
14. Use the mouse to move the Sensitivity window to an easily accessible section of the screen.
15. Select Applications from the menu bar.
16. Choose Emission Wavelength Scan from the drop down menu.
17. Set Lower limit to 380nm.
18. Set Upper limit to 430nm.
19. Set Scan rate to 1.00.
20. Set Repetitions to 1.
21. Click the mouse pointer on the OK button.
22. Select Applications from the menu bar.
23. Choose Start Applications from the drop down menu.
24. Change the file name from SPEC001 to RAMAN1.
25. Click the mouse pointer on the Start button.
NOTE:
At this point the instrument should be performing an emission scan.
26. The screen should show a data window similar to that of Figure 2 when the scan has
completed.
Figure 2 Data Window
27. Select View from the data window menu bar.
28. Select Cursor from the drop down menu.
6
29. Move the mouse pointer to the Display Cursor box and click. A check should appear in the
box.
30. A crosshair cursor should now be visible inside the data window. The position of the vertical
line is the one we will be concentrating on.
31. Move the mouse pointer to the highest point of the Raman spectrum and click. The cursor
should move to the peak.
32. Note the X and Y values displayed in the corner of the data window.
33. Use the left and right arrow keys of the keyboard to locate the highest Y value that can be
attained on the spectrum. The corresponding X value at this point should be 397nm (plus or
minus 1.0nm).
34. Obtain a printout of the spectrum to attach to your copy of the Installation Report.
35. If the peak of the Raman line occurs as stated in step 32, the monochromators are within
calibration. If the peak does NOT occur within these limits, you must perform part two of the
monochromator calibration procedures found in the Alignment section if this manual.
36. Leave the cuvette of distilled water in the sample chamber. It will be needed to perform the
Signal-to-Noise ratio test.
Perform Signal-to-Noise ratio test
1. Place the top cover back on the instrument.
2.
Select Applications from the menu bar.
3.
Choose Emission Wavelength Scan from the drop down menu.
4.
Set the lower limit to 355nm.
5.
Set the upper limit to 500nm.
6.
Set the scan rate to 1.00.
7.
Set the repetition to 1.
8.
Click the pointer on the OK button.
9.
Select Applications from the menu bar.
10. Choose Start Application from the drop down menu.
11. Change the filename from SPEC0001 to RAMAN2.
12. Click to mouse pointer on the Start button.
NOTE:
At this point the instrument should be performing an emission scan.
13. The screen should show a data window similar to the one shown in Figure 2 when the scan
has completed.
14. Choose File from the RAMAN2 data window menu bar.
7
15. Select Export as from the drop down menu.
16. Click Setup on the Export As window
17. Select ASCII and click OK
18. Select the file to export “Em scan” and click Export.
19. Start a command line session by selecting Start –Run and entering “command /k” for
Windows 98 and earlier systems, or “cmd /k” for Windows NT, 2000 and XP, then selecting
OK.
20. Change to the directory containing the RAMAN2.TXT by entering “CD C:\Program
Files\AB2” (use quotes) on the command line.
21. At this point the RAMAN2.TXT and SN.EXE files must be in the same directory.
22. Type the following command: SN RAMAN2.TXT.
23. Press the ENTER key for the operation to begin.
24. The screen should respond with a numerical value that will represent the Signal-to-Noise
ratios of the instrument. Both Peak-to-Peak and RMS values are provided. This product is
specified in Peak-to-Peak, but the competition uses RMS. Use the more favorable RMS
values for comparison purposes.
25. Record the Peak-to-Peak value.
Complete documentation
1. Locate the Installation Report form at the end of this service manual.
2.
Record the following information on the Installation Report:
EX calibration value
EM calibration value
Signal-to-Noise test value
Accessories with serial number
Date of installation
Customer’s address information
Technician’s address information
•
•
•
•
•
•
•
3.
Note any faulty components or problems with the instrument. It usually is best to NOT record
this information on the customer’s copy.
Demonstrate instrument operation
NOTE:
Before beginning the demonstration, secure the top cover of the computer, power
down the instrument, and remove the top cover of the instrument. It is best to let
the lamp cool down for approximately 15 to 20 minutes before reigniting it. This
provides an excellent time to take a break or call the shop for messages.
The following information is provided as an aid to giving the customer a demonstration of the
instrument operation. You can tailor the demonstration as you see fit. Keep in mind that this is
one of the most important steps in the successful installation of a unit.
8
1.
Demonstrate a lamp change operation.
2.
Briefly explain the operation of the major components and how they interact together.
3.
You may want to show them a diagram of the light path.
4.
Show them how to remove and replace the top cover.
5.
Power up the instrument. Show the customer the following:
a. Lamp Enable switch on left end panel.
b. Lamp Monitor light on front panel.
c. Reset Lamp on right side panel. Explain YELLOW, GREEN and RED status indicators of
this LED. Also explain operation of COLD and WARM RESET switch options.
d. AUX1, AUX2, PMT MONITOR, TTL IN and TTL OUT connectors on the right side panel.
Explain their operation.
6.
Power up the computer, monitor and printer.
7.
Briefly explain mouse techniques. Show the customer how to size windows and move them
around on the screen.
8.
At this point, it is usually best to let the customer take control of the mouse. Explain the items
as requested or needed.
9.
Have the customer select the AB2 software.
10. Point out the “Initializing Instrument…” message at the lower left corner of the screen. Also
point out the sounds that are emitted from the instrument during this phase of operation.
Explain the significance of the instrument sounds.
11. At this point, the most important information that the customer needs to know is how to do
the following tasks:
a. File management techniques.
b. Sending output to the printer.
c. How to do instrument setup.
d. How to select the appropriate application that they want to perform.
e. How to display and control the cursor inside a data window.
f. The differences between a data window and the main window.
g. How to do menu bar selections. Explain the differences between data window menu bar
selections and the main window selections.
12. Explain as much about the other functions of the menu bar as you can. Some items only
need to be covered briefly. Experience, in this case, is the best teacher of knowing how to
use them.
13. Tell the customer how they can get technical or applications assistance. Conclude your
presentation.
9
System Description
Overview
This instrument features many hardware capabilities in a compact design. All internal parts are
factory installed, including the card cage, power supply, Filter Wheels (optional), sample
chamber, two monochromators, and its one-of-a-kind dual light source. The dual light source is
optional and available in three distinct configurations:
•
A continuous wave 150 watt xenon lamp
•
A 7 watt pulsed xenon lamp
•
A combination of both continuous wave and Flash Lamp
The dual light source design features a 150-watt ozone-free xenon lamp for high-sensitivity
(greater than 900:1 Peak-to-Peak Signal-to-Noise ratio) fluorescence measurements and/or 7
watt pulsed xenon lamp for phosphorescence measurements. In addition, the light path
configuration and optimized beam geometry combine with the illumination system to enhance
sensitivity.
The instrument makes a full range of standard measurements, such as corrected excitation
spectra, time trace, intracellular probe measurement, quantitative analysis, and more. Emission
correction factors are generated for each instrument before it leaves the factory.
A monochromator slew rate of 12, 000nm per minute suits multiple wavelength measurements.
Bandpass settings of 0.5 to 16.0nm are computer-controlled for accuracy and reliability.
Standardization of the T-Optics sample chamber allows for compatibility with a full line of
accessories. These include Filter Wheels, an automated sipper sampler, fixed and variable angle
front surface accessories, additional PMTs, and flow cells for HPLC or other continuous flow
applications. All accessories are designed to simplify sample handling in a variety of situations.
A mouse-driven graphical interface enables high-speed computer-instrument communication in a
multitasking environment. Online help text and step-by-step acquisition setups provide for
complete user control of the instrument from the computer keyboard. Personalized software
setups, libraries of these setups, and other customizing features of the software help automate,
organize and standardize your data acquisition.
The small footprint of the instrument results in a compact system configuration where the
computer can sit on the load bearing (200 lbs.) top surface.
Instrument system components
Light source
The dual light source design features a 150 watt ozone-free xenon lamp for high-sensitivity
fluorescence measurements and/or a 7 watt pulsed xenon lamp for phosphorescence
measurements. The continuous xenon lamp has an elapsed time meter for monitoring total lamp
usage. The lamp assembly consists of a fan-cooled housing and a replaceable power supply.
Only a six-inch clearance is required for lamp cooling. The Flash Lamp can be both focused and
aligned. The lamp assembly is available from the factory for field installation.
Monochromators
Fast-slewing monochromators (12,000nm per minute) and computer-controlled bandpass settings
(0.5nm to 16nm) provide for rapid multiple wavelength measurements. Both excitation and
emission monochromators feature 1200 line/mm, ion-etched, concave, holographic gratings in a
modified Seya-Namioka design with 200mm focal length.
10
Sample compartment
The sample compartment has a removable front plate and lid. The optics has a two-inch focal
length on standard units.
The T-Optics compartment design allows for standardization with other accessories. The single
cell sample holder is standard.
•
Stopped flow apparatus
•
Front surface accessory
•
Variable angle coverslip holder
•
Automated programmable sample holders
•
Programmable sipper sampler
Filter/lens holders
The holders accommodate standard two by two inch filters to filter out undesirable light.
Circuit Description for the High Voltage and Monochromator Driver Board
Overview
The SQ-305 high voltage and monochromator driver board is a double-wide printed circuit board;
(i.e., with two 96-pin plug connectors) which plugs into connectors P4 and P5 on the electronics
card cage assembly.
The primary functions of this board are:
•
High voltage bias for the photomultiplier tube
•
Power control to the monochromator slit motor
•
Power control to the monochromator grating motor with high BOOST power and lower
HOLD or (SLEEP) power
•
Power to solenoids for the shutters and lamp mirror
High voltage
The high voltage bias for the PMT is generated by the MIL VH-15 DC-to-DC converter and
support circuitry, which delivers an extremely stable high voltage of up to –1250 VDC at the HV
output.
The CPU controls the HV level by directly programming the output voltage of the AD558 ADC
(U3). The output voltage of the U3 indirectly controls (through the relay M2, amp X7 transistor Q4,
and choke L1) the voltage level applied to the IN+ input of the VH15 HV brick. The brick steps up
the voltage at its input to deliver high voltage at its OUT- pin (this voltage is attenuated through
R14, R15 and R16 to provide negative feedback to amplifier X7). The HV is then filtered by C10
and T1 before reaching the HV output (HV-), which is then delivered directly to the PMT. The HVis also greatly attenuated through R18 and sent to the CPU board (as HV-SENSE) for diagnostic
purposes.
Slit motor control
The circuit centers around two Motorola SAA1042 stepper motor driver ICs, which drive the
motors directly. U1 controls the excitation slits and U2, the emission.
Each driver IC receives its commands from the CPU board via two control lines. The UP/DOWN
line (pin 10) determines which direction the motor will turn, and the number of pulses received on
the STEP line (pin 7) determines the number of steps taken in the appropriate direction. Outputs
L1 through L4 are connected directly to the windings of the two-phase bipolar stepper motor and
to the protection diodes (D1 to D8). This circuit is configured for FULL STEP mode.
Figure 3 Slit Motor Control, shows a timing diagram indicating the 4-step pattern of the SAA1042s
in this circuit.
11
STEP
U/D
L1
L2
L3
L4
Slit Motor Control
Figure 3 Slit Motor Control
Grating motor control
The grating driver circuit uses a pair of Motorola SAA1042 ICs (U4 and U5) in a manner similar to
the slit motor control circuit. However, these driver ICs are not rated for the additional current
required by the larger grating motors and cannot be used to drive them directly. Also, the grating
motors are UNIPOLAR stepper motors (current flows in only one direction in any given winding),
which are being driven in HALF STEP mode. Therefore, additional circuitry is required and the
output step pattern of the SAA1042s is somewhat different. Figure 4 Grating Motor Control shows
the 8-step pattern when the UP/DOWN line (pin 10) is low. The pattern is reversed when the
UP/DOWN is high. This step pattern is delivered to the UDN2878W drivers (X5 and X6) through a
set of AND GATES (U10 and U11).
STEP
L1
L2
L3
L4
Grating Motor Control
Figure 4 Grating Motor Control
This circuit incorporates the use of two different output power settings. Due to the design of the
monochromators, very little HOLDING torque is necessary once the grating has been moved to
the desired position. Therefore, a 600-micro-second boost of power is delivered to the motor
every time it moves a step.
Whenever the motor is stationary, the circuit goes to idle or SLEEP mode. When in SLEEP mode,
the power being delivered to the motor windings is pulse width modulated with a 15% duty cycle.
12
Solenoid control
Three 2N6548 transistors drive the three rotary solenoids in the instrument:
1. Q1 drives the EX shutter.
2.
Q2 drives the EM shutter. The sample chamber lid switch must be closed to enable this
shutter.
3.
Q3 drives the CW/Flash Lamp mirror.
Filter/Polarizer Wheels Driver Board
Circuit description
The SQ-306 Filter/Polarizer wheel driver board will be installed into only those units where the
Filter/Polarizer wheel option is desired. This driver board comes complete with a single 96-pin
plug connector which mates with P6 on the card cage assembly.
The sole purpose of this board is to drive the motors that move the Filter/Polarizer wheels. The
circuit centers around two Motorola SAA1042 STEPPER MOTOR DRIVER ICs, which drive the
motors directly. U1 controls the excitation wheel and U2 the emission.
Each driver IC receives its commands from the CPU board via two control lines. The UP/DOWN
line determines which direction the motor will turn, and the number of pulses received on the
STEP line determines the number of steps taken in the appropriate direction. Outputs L1 through
L4 are connected directly to the windings of the 2-phase, bipolar stepper motor. This circuit is
configured for FULL STEP mode. Figure 5 Filter Motor Control shows a timing diagram that
indicates the 4-step pattern of the circuit.
STEP
U/D
L1
L2
L3
L4
4-Step Pattern of Circuit
Figure 5 Filter Motor Control
PMT
Light emerges from the sample chamber, passes through the emission monochromator or a filter
and enters the PMT. The light from the emission monochromator illuminates the photocathode of
the PMT. The PMT then converts the light to an amplified DC current, which is measured by the
instrument’s acquisition electronics.
13
Monochromators
Monochromators are precision optical components consisting of diffraction gratings, mirrors, and
slits used to select wavelength and bandpass values for exciting and emitted light. The
monochromators are 200mm with an ultra-precision sinusoidal drive.
Monochromators are the optical elements that allow continuous selection of a pure narrow band
of excitation light or emitted light. The monochromators exhibit a modified Seya-Namioka design
that incorporates internal baffles to improve the stray light rejection. The grating has a focal length
of 200mm from the entrance slit to the grating. The exit side of the monochromator has a focal
length of 188mm.
Bandpass
Bandpass selection is made in the software program at the Setup Instrument Setup
Monochromators dialog box.
The bandpass is the width of the slit in nanometers (0.5-16nm). Bandpass affects the intensity
and resolution of light emerging from the monochromator. As the bandpass is opened, sensitivity
is increased, since more light is passed to the sample.
Selectivity, however, is reduced, since the wavelength range of light passed is widened. This
results in a trade-off between the ability to detect low levels of light and the ability to resolve
closely spaced peaks in a spectrum. The goal of setting bandpass is to balance intensity and
resolution for a particular application.
Computer
Required:
•
A personal computer with 100 MB of free space on its drive
•
64MB RAM memory
•
CD drive
•
SVGA color monitor with SVGA graphics adapter card
•
2-button mouse pointing device (e.g., mouse, trackball, touch pad)
•
Windows 95 or later
•
GPIB (IEEE-488) interface card for computer-instrument communication
Printer and plotter
Spectral plot output and text may be directed to a variety of laser printers for high resolution,
publication ready data in addition to spectral plot output on graphic printer/plotters and digital
plotters.
Accessories
Since the sample compartment is based on a standard T-Optics design, these accessories are
available:
•
The single cell, non-stirred sample holder (standard)
•
Programmable sipper sampler
•
Flow cells for HPLC or other continuous flow applications
•
Variable and fixed angle solid sample holders
•
Variable angle coverslip assemblies for Live Cell Perfusion Studies
•
Low temperature cell holder for liquid nitrogen/helium temperature work
•
Glan prism polarizers
•
Interface with sate-of-the-art stopped flow apparatus
•
Microcells
•
Auxiliary PMT with housing
•
Two- and four-cell thermostattable sample holders with magnetic stirring and automatic
control
14
•
•
•
Sample compartment adapter for low volume samples in a standard cuvette
Circulating water bath
2x2 filters for a range of wavelength selection requirements
Overview of power up diagnostic functions
Diagnostic functions are performed at POWER UP before any communications are initiated to the
instrument. The RESET LED, located on the right side end panel of the instrument, gives the
following indications.
YELLOW
while the diagnostics are being performed.
RED
if one of the diagnostics fails.
GREEN
if all the diagnostics pass.
NOTE:
If the Led is RED after the diagnostics finish, it may indicate an instrument failure.
CPU/Analog Board Circuit Descriptions
Circuit descriptions
The CPU/ANALOG BOARD is the three-wide PCB with a row of LEDs at the top and occupies
the front slot of the card cage. This board contains an analog front end and a microprocessor
(CPU) with its repertoire of supporting circuits that serves as the controller of the instrument. A
host computer operates the instrument by sending commands to the CPU via a high-speed
parallel bus – the IEEE-488 GPIB. The CPU interprets and executes these commands and sends
any data/status back via the same bus. Peripherals (e.g.,, monochromators and A/D converter,
etc.) are controlled by the CPU through memory-mapped I/O registers. Some I/O registers are
implemented by TTL tri-state latches and drivers, and some are embedded within special function
LSI chips, such as the TMS9914A GPIB interface.
Figure 41 CPU/Analog Bd. Schematic – Digital Section: CPU, RAM, ROM, PI/T and address
Decode
The core of the system comprises an MC68B09 microprocessor (U1) clocked by an 8MHz DIP
oscillator (U10), a 2K Loader PROM (U9) and 60K SRAM (U7 and U8). The remaining 2K of
address space is assigned to I/O registers. To allow an In-circuit Emulator to take control of the
board during program development, the 2K PROM can be switched into 2K SRAM by removing
the ROM-EN jumper. After power-up (or a cold reset from the I/O panel), the Loader first sets a
tri-color LED on voltage test. If the test is successful, the Loader will turn the LED to green and
wait for the operating system program to be downloaded via a GPIB port (see sheet4). 30K of
SRAM is reserved for the operating system and the remaining 30K for data buffer. If the test fails,
the LED will turn red to indicate that there may be problems. This simple procedure assures that
the core is healthy, the Mono/HV board is present by responding to the proper high voltage
settings, and major parts of the analog front end (see sheet 2) are also working. Any problems
detected here have to be corrected before proceeding.
With the operating system loaded in SRAM, the CPU can carry out commands to operate the
instrument. The Parallel-Interface/Timer chip (PI/T), U6/MC68230, provides an abundance of TTL
compatible I/O lines and a 24-bit timer. The I/O lines are used for:
•
Movements of the grating and slit of both monochromators.
Signal names are coded according to their functions.
e.g.,, EXG-STEP^ = EX mono. Grating STEP.
(U)/D = Up/Down; direction of movement.
EMS-CAL = EM mono. Slit CAL point.
UL^ and LL^ = Upper and Lower limits.
•
High voltage setting: HV7-HVO
•
Tri-state driver control: DR-EN^ (see sheet 3).
•
Interrupt request from the timer: PTI-IRQ^.
•
Reference photodiode gain control: PD-G1 and PD-G0.
15
•
Note:
ROM enable/disable by software: ROM-EN.
An active low signal is identified by the signal name followed by a ^
The 24-bit timer precisely controls the timing of a scan. Driven by the 8MHz clock divided down
by an internal 5-bit prescaler, the timer can interrupt the cup on the FIRQ^ line at intervals
determined by the 24-bit word with a four microseconds resolution. The rest of the circuits on this
sheet handle most of the address decoding. The PI/T has an asynchronous bus interface that
may require an extended bus cycle. During a bus cycle that accesses the PI/T, a D-flip-flop,
U18A/74LS74, stretches the cycle until the chip acknowledges that it is ready by activating the
DTACK^ line.
Figure 42 CPU/Analog Bd. - Analog Section: Analog Front End
This section conditions in-coming analog signals and converts them into digital numbers for
processing by the CPU. These signals include:
• PMT data (PMT-IN)
• Reference photo diode data (PD-K)
• Two auxiliary analog inputs (AUX-1 and AUX-2) and
• A high voltage sensing line (HV-SENSE)
An analog multiplexer allows the CPU to pick one out of many channels to be digitized. To
monitor the signal from the PMT (to determine the scan time of a phosphorescence decay for
instance), the PMT data is buffered to generate an analog output, PMT-MON, on the I/O panel.
Unless otherwise specified, the voltage range for analog circuits is +/-10V min.
PMT-IN is a minute current coming from the anode of the PMT. To produce a programmable
hardware offset for the PMT signal, an offset current controlled by a 16-bit DAC
(Digital-to-Analog Converter), U47/DAC1600KP-V, is added to the raw PMT current. The total
current is then converted to a voltage by a high-speed low-bias-current op-amp, U31/AD711,
through a 100 M Ohm feedback resistor, 32. The op-amp in turn feeds three different ICs to
generate three outputs:
• U37/AD-OP27, for the analog output PMT-MON;
• U34/5151-1K, a 1KHz low-pass filter for low-noise CW lamp fluorescence
measurements; and
• U36/HA1-5330, a high-speed sample-n-hold amplifier for catching Flash Lamp
data.
The last two outputs are ready to be multiplexed and digitized.
PD-K, the photo diode input, is also a current source. This signal is taken from the cathode to
keep the polarity consistent with the PMT channel. Since this is a reference channel, there is no
need for programmable offset. The same type of op-amp, U30/AD711, performs current-tovoltage conversion. A programmable gain of 1/10/100 is provided through the feedback loop
(U38, R40, R41 and R22) because the photo diode has no gain control. Similar to the PMT
signal, a low-noise channel (U33/5151-1K) and a high-speed sample-n-hold channel
(U35/HA1-5330) are generated and fed to the multiplexer.
AUX-1 and AUX-2 are auxiliary analog (voltage) inputs from the I/O panel. AUX-1 is an
un-buffered input that is suitable for high-level analog signals. AUX-2 has a gain of 10 and may
be used for transducers with low level outputs. HV-SENSE is the PMT high voltage bias
attenuated (1/126) to within +/- 10V. The CPU, knowing the attenuation ratio, can monitor the
actual bias voltage for diagnostic purposes. All three channels need to be multiplexed and
digitized.
16
To convert analog voltages into binary numbers comprehensible by microprocessors, we need a
digitizer. U22/AD1380 is a high-speed, +/- 10V, 16-bit successive approximation A/D
(Analog-to-Digital) converter with internal clock, reference and sample-n-hold. The analog
multiplexer, U32/ADG528A, selects 1 out of 8 channels as output to the digitizer through a buffer,
U29/AD711. There are a total of 7 possible channels mentioned above. The analog ground is
chosen as the last channel for diagnostic purposes. The following is a list of the 8 channels:
S8
S7
S6
S5
S4
S3
S2
S1
=
=
=
=
=
=
=
=
PD with sample-n-hold
PMT with sample-n-hold
HV-SENSE
AUX-2 (gain of 10)
AUX-1
PD with 1KHz low-pass filter
PMT with 1KHz low-pass filter
Analog ground.
It takes a total of 22 microseconds for a complete A/D conversion, which includes channel
switching, acquisition of the internal sample-n-hold and digitization. The actual throughput will be
slower due to software overhead.
Figure 43 CPU/Analog Bd. - Flash Lamp Control: Flash Lamp and Misc. Control
Unlike the CW lamp, the Flash Lamp only excites the sample by pulses of light. The resulting
PMT and photo diode signals thus are also pulses, which have to be latched by high-speed
sample-n-hold amplifiers (see U35 and U36 in Figure 42 CPU/Analog Bd. - Analog Section).
Flash Lamp acquisitions require more complicated timing control. Three extra timers are provided
for these specific timing tasks:
Timer 2 = period between flashes.
Timer 1 = delay of the first PMT data point.
Timer 0 = interval between data points of a phosphorescence decay after the
first data point.
Each timer consists of a prescaler and a counter. The prescaler divides an 8MHz basis frequency
by a programmable 16-bit number to set the counting frequency of another programmable 16-bit
counter. This cascading arrangement effectively creates a 32-bit timer capable of counting from 1
microsecond/cycle to minutes. Software can also extend the count period indefinitely. All three
prescalers reside in U48/82C54, and counters in U49/82C54. For example, pins CLK2, OUT2 and
GATE2 of U48 are the input, output and gate of the Timer 2 prescaler; while the same pins on
U49 are those of the matching counter. A typical Flash Lamp acquisition is shown below to
illustrate how the timers interact with the rest of the circuits.
A) Load the period between flashes in Timer 2, first point delay in Timer 1 and
interval between points in Timer 0. Start Timer 2 by activating T2-GATE.
B) Simultaneously trigger a flash and start Timer 1 using lines: FLASH^ and
T1-GATE. If masking of high voltage during the flash is necessary, HV-MASK^
has to be turned on ahead of time.
C) After a fixed hardware delay (U28/74LS393), the photo diode signal is latched by
PD-HOLD for digitizing.
D) When Timer 1 completes the first cycle, T0-GATE becomes active to start
Timer 0. At the same time, C76 couples the falling edge of T1-DONE^ to turn T0DONE^ on; thus activating PMT-HOLD to latch the first data point.
E) Software digitizes the first data point then de-activates T0-DONE^ and waits for
the next data point if more than one data point is needed. Else go to step (G).
F) From this point on, Timer 0 determines the intervals of subsequence data points.
At the completion of a timing cycle, Timer 0 activates T0-DONE^ and latches the
PMT signal by the PMT-HOLD line. Software digitizes the data point,
17
de-activates T0-DONE^ and again waits for the next data point. This process
repeats until the total number of points per flash is collected. Timers 1 and 0 can
then be turned off.
G) The second flash occurs when Timer 2 completes its timing cycle and activates
T2-DONE^. Software de-activates T2-DONE^, repeats steps (B) through (F) and
waits for the third flash and so on. The acquisition is completed when the
required number of flashes is done.
Three of the I/O registers are implemented by discrete octal TTL latches/drivers. U50/74ALS564
supplies Flash Lamp related control lines and U51/74ALS564 is a miscellaneous control register.
During the power-up sequence, DR-EN^ disables the drivers of both chips until the correct control
bits are loaded. Signal names are listed below:
A)
B)
C)
D)
E)
LH-MCW^
HV-MASK^
FLASH^
PMT-HOLD^
T2-GATE
=
=
=
=
=
F) HV-ON^
G) EXW-STEP
=
=
H) TTL-OUT^
I) EX-OPEN
=
=
J)
LED-R^
=
K) LED-G^
=
Lamp High and Mirror in CW position.
High Voltage MASK.
trigger a FLASH.
set the PMT sample-n-hold to HOLD mode.
a GATE to start/stop Timer 2; the same applies to T1-GATE
and Timer 1.
to turn High Voltage ON.
EXcitation Filter Wheel STEP; the same applies to EMWSTEP and EMission Filter Wheel.
a TTL compatible digital OUTput line.
EXcitation and shutter OPEN; the same applies to EM-OPEN
EMission shutter.
turn on the Red color of the tri-color LED mounted on the I/O
panel.
turn on the green color of the LED.
U52/74HCT563 buffers a random collection of status lines onto the CPU data bus by tri-state
drivers. Signal names are listed below:
A) AD-DONE^
B) EXW-CAL^
C) TTL-IN^
D) PD-HOLD
E) T2-DONE^
= an A/D conversion is DONE.
= EXcitation Filter Wheel CAL. point; the same applies to
EMW-CAL^ and EMission Filter Wheel.
= a TTL compatible digital INput line.
= Photo Diode sample-n-hold is in the HOLD mode.
= a timing cycle of Timer 2 is DONE; the same applies to
T1-DONE^/T0-DONE^ and Timers 1/0.
Other circuits/components on sheet 3 include: one of the 96-pin connectors, 3, and six BCD
rotary switches, U53-U58, that store the calibration point wavelengths of the monochromator
gratings. Each switch represents a decimal digit. Labeling the digits I to N from left to right, the
cal. points are defined as:
Ex. mono. cal. point = -IJ.K nm
Em. mono. cal. point = -LM.N nm
Figure 44 CPU/Analog Bd. - GPIB Section: GPIB port and Misc. I/O
The instrument communicates with a host, usually a PC, via a high-speed parallel bus – the
IEEE-488 GPIB. The bus consists of 16 TTL level lines, 8 for control and 8 for data. There is also
a set of bus protocol that each number of the bus has to implement. To identify the members on a
bus, each member is assigned a unique 5-bit address. Secondary addressing expands the
address space beyond 5 bits. Detailed descriptions of the GPIB can be found in published
manuals.
18
Because of the wide acceptance of the GPIB, bus controller chip sets are readily available. An
LSI chip, U61/TMS9914A, implements the bus protocol. It requires a 4MHz clock and can
interrupt the CPU via the GP-IRQ^ line. For bus transceivers, the pair U60/75ALS160 and
U59/75ALS162 interface with the data and control lines respectively. An OR gate, U17/C,
switches the tri-state data bus drivers to open-collector during parallel poll. GPIB bus address is
selected by jumpers J5-J1. The instrument is assigned a binary address of (00001); J5-J2 out
and J1 in. As an aid in diagnosing the bus, a row of Quad-LEDs, U65-U69, shows the status of all
16 bus lines and 2 related internal signals TE and CIC^; a spare LED serves as a +5V indicator.
Two more Quad-LEDs, U63 and U64, show the status of sensors associated with calibrating
stepper motors: Lower Limit, Grating CAL., Slit CAL and Wheel CAL. Other circuits/components
on sheet 4 include: connectors P1, P2 and P4, +5V and +12V regulators, +12V to +/-15V DC/DC
converter and decoupling capacitors.
Figure 6 I/O Panel - Instrument Right Side
19
Lamp On/Power Delay Board
Circuit description
The LAMP ON & POWER DELAY Board is located within the SQ-302 Power Supply.
The Primary functions of this board are:
LAMP ON – Controls the CW Lamp power supply.
POWER DELAY – Prevents power from being applied to the electronics cage until
AFTER the CW Lamp is ignited.
HOUR METER – Enables the operation of the meter while the CW Lamp is on.
LAMP MONITOR – Illuminates the LED when the CW Lamp or the Flash Lamp is in
operation.
Lamp on
This circuit controls the CW Lamp by interrupting the power (120 VAC) applied to the CW Lamp
power supply. When the LAMP ENABLE Switch on the SQ-302 power is in the ON position,
power is applied to the CW Lamp power supply when the instrument is powered up. The Lamp
power supply then attempts to ignite the lamp, if the IGNITE control (pin 3 of P9) is in a high
state.
Power delay
In order to avoid disrupting the electronics, it is necessary to avoid igniting the lamp with power
applied to the electronics cage. Therefore, the circuit is designed so that the LAMP SENSOR
must detect light from the lamp before power is applied to the card cage. (This safety feature is
disabled when the LAMP ENABLE switch is moved to the OFF position.) Once the LAMP
SENSOR detects light, transistor Q3 conducts causing relay M1 to be energized. The closing of
M1, in turn, causes 3 events to occur:
A.
B.
C.
+RAW (+19vdc) is applied through choke L2 to the electronics card cage.
Q4 conducts which keeps relay M1 energized – even if the lamp is shut off.
Q5 conducts which brings the IGNITE enable line low (pin 3 of P9). This
prevents the CW Lamp power supply from igniting the CW lamp while power is
applied to the cage.
Hour meter
The Hour Meter keeps track of the CW LAMP’s time in use. When the LAMP SENSOR detects
light, transistor Q6 conducts and creates the power conditions necessary to run the Hour Meter.
Lamp monitor
A LAMP MONITOR LED on the front skirt of the instrument indicates when the lamp is operating.
When the LAMP SENSOR detects light, transistor Q1 conducts and causes the LAMP MONITOR
LED to light.
The LED glows brilliantly as long as the CW Lamp is lit. When the light source is the Flash Lamp,
however, the duration of the flash is too short to be seen by the human eye. Therefore, a circuit
has been added to stretch the pulses.
When the LAMP SENSOR detects a flash, Q1 conducts just long enough to trigger the NE555
timer (U1). Each time the timer is triggered, it lights the LED (through Q2) for approximately 5
milliseconds.
20
I/O
PANEL
GPIB BUS
TTL - IN
TTL - OUT
PMT MONITOR
AUX1
AUX 2
AST
NMI
RED/GRN/YEL
COLD
WARM
RESET
SWITCH
LED
LAMP HI/LO
LAMP ON
POWER DELAY
PCB IN P/S
FLASH TRIGGER
CALIBRATION STATUS
CPU
AND
ANALOG
BOARD
LIMIT AND CALIBRATION STATUS
SIGNAL
REF
DIODE
SIGNAL
PMT MASKING
H.V.
AND
MONO
BOARD
CW LAMP
P/S
FLASH LAMP
P/S
CW
LAMP
FLASH
LAMP
PMT
LAMP
MIRROR
H.V. BIAS
LAMP SELECT
OPEN/CLOSE
MOTOR CONTROL
EX & EM
SHUTTERS
EX & EM
MONOs
FILTER
WHEEL
BOARD
MOTOR CONNECTOR
FILTER
WHEELS
Figure 7 Block Diagram
21
LAMP
SENSOR
LAMP
MONITOR
LED
ADDRESS
DECODING
RAM/ROM
GPIB PORT
MISC I/O
& CTRL
ANALOG
ACQUISITION
FLASH LAMP
CONTROL
DEVICE SELECT LINES
CONTROL BUS
CPU
DATA BUS
ADDRESS BUS
8MHZ
CLK
PARALLEL I/O & TIMER (PI/T)
GRATING
UL & LL
SENSING
GRATING
& SLIT STEP
LINES
GRATING
& SLIT CAL
SENSING
H.V.
LEVEL
CONTROL
MISC.
CONTROL
LINES
AB2 CPU & ANALOG BOARD
Figure 8 CPU & Analog Board Block Diagram
22
BLOCK DIAGRAM
BUFFER
LOW
PASS
FILTER
BUCKING
CURRENT
PMT - IN
DATA BUS
FROM
FLASH
LAMP
CONTROL
REF - IN
FROM
PI / T
PORT C
AMP
SAMPLE
&
HOLD
16-BIT
OFFSET
DAC
DATA
LATCH
16-BIT
A TO D
CONVERTER
FROM HV
CIRCUIT
DIGITIZED
DATA
DATA
LATCH
PMT-HOLD
SAMPLE
&
HOLD
PD-HOLD
PD-K
LOW
PASS
FILTER
AMP
BUFFER
PROGRAMMABLE GAIN CONTROL
AMP
FROM
I/O
PANEL
TO
I/O
PANEL
PMT MONITOR
AUX 2
CHANNEL
SELECT
MUX
GAIN = 10
AUX 1
HV SENSE
ANALOG ACQUISITION
Figure 9 Analog Acquisition
23
DATA BUS
DATA BUS
PROGRAMABLE
START TIMER 2
GATE
TIMER
2
DONE
PROGRAMABLE
FROM
CPU
T2 DONE
DONE
T1 DONE
T0 DONE
PROGRAMABLE
GATE
STATUS
LATCH
GATE
DATA BUS
START TIMER 1
TIMER
1
TIMER
0
DONE
START TIMER 0
PMT - HOLD
SOFTWARE ACTIVATED HOLD
CONTROL
LATCH
TIMER
TO
ANALOG
SECTION
PD - HOLD
FLASH
TRIGGER
DRIVER
CW/FLASH LAMP SELECT
FLASH LAMP
CONTROL
HV-MASK CONTROL
Figure 10 Flash Lamp Control
24
TO
FLASH
LAMP
TO
I/O
SECTION
TO PMT
HV-ON
NEGATIVE FEEDBACK
ATTEN.
ON/OFF
AMP
D/A
CONVERTER
LOW PASS
FILTER
LOW PASS
FILTER
HIGH VOLTAGE OUT
HV DATA & CONTROL BUS
RELAY
ATTEN.
PULSE
GENERATOR
TO PMT
HV SENSE
TO CPU
600 uS PULSE
1 PULSE PER
STEP
(BOOST POWER)
L1
EXG (EMS)
STEP
FROM
CPU
UP/DOWN
MOTOR
DRIVER
L1
L2
L2
L3
PULSE - WIDTH
MODULATION
L3
L4
L4
L1
POWER
DRIVERS
L2
L3
L4
L1
L2
L3
OSC.
PULSE
GENERATOR
15% DUTY CYCLE
SLEEP CIRCUIT
(NOT MOVING)
(HOLDING POWER)
L4
TO
GRATING
MOTOR
WINDINGS
TO
SLIT
MOTOR
WINDINGS
EXS (EMS)
STEP
MOTOR
DRIVER
UP/DOWN
AB2 HV & MONO BOARD
BLOCK DIAGRAM
Figure 11 HV & Mono Board Block Diagram
25
CURRENT
LIMITING
DIODES
D1-D4
EXW-PH1
EXW-STEP
MOTOR
DRIVER
EXW-PH2
EXW-PH3
L11
TO EXCITATION
FILTER WHEEL
EXW-PH4
FROM CPU
BOARD
(U) / D
CURRENT
LIMITING
DIODES
D5-D8
EMW-PH1
MOTOR
DRIVER
EMW-STEP
EMW-PH2
EMW-PH3
L12
TO EMISSION
FILTER WHEEL
EMW-PH4
FILTER WHEEL CONTROL BOARD
BLOCK DIAGRAM
Figure 12 Filter Wheel Control Board Block Diagram
26
Troubleshooting Guide
SYMPTOM
CW lamp is flickering
WARNING:
REMEDY
CW lamp is failing
RESULT
Call factory for a
replacement.
When the cover is off the instrument, DO NOT look directly at the lamp. Try to
direct your gaze around the lamp.
Lamp flickering coincides
with the pulsing of an
abnormal Time Trace.
CW lamp does not fire on
the first try.
Turn the power off and back
on 3 or 4 times.
Signal-to-Noise ratio (900:1)
of Raman band of water
drops off.
CW lamp is oscillating
No signal.
Try adjusting the lid on the
sample holder in case it is
ajar.
Check to see if the interlock
switch is sticking. You may
be able to free the switch by
clicking it with your finger.
Sticky shutters.
Lubricate the shutter with
graphite, see Figure 1
Shutter Lubrication Points
Adjust the shutters. Loosen
the two hold-down screws
that hold the shutter solenoid
to the base plate. This
adjustment will affect the
shutter movement.
Slits on the EX or EM
monochromator will not
calibrate
Open the bottom cover of
the monochromator to get
access to the slits. Access
the software program.
Setup-Instrument
Setup-Send Commands
and enter EX:SLITCAL. Try
the dowel pin on both ends
of the monochromator. If the
slid does not calibrate, turn
the large screw next to the
slit motor until the slit
calibration hole on each end
and the slit aperture are
aligned.
27
If unsuccessful after 3 or 4
tries, contact the factory for a
replacement.
You will get the expected
signal.
SYMPTOM
Light stays red when you
perform an instrument “cold
start.”
REMEDY
When you press RESET
switch UP, the light should
turn alternately yellow (wait)
then green (OK). If the light
turns and stays red (NOT
OK), contact the factory for
service.
RESULT
Signal bar on Instrument
Setup screen pulses (like a
heartbeat) with a noticeable
pattern.
The CW lamp is oscillating.
Call the factory for a
replacement.
A Time Trace for a Ramen
line fluctuates wildly. A
normal line should be fairly
steady.
*See the data sets below for
examples of normal
oscillating time traces.
The CW lamp is oscillating.
Call the factory for a
replacement.
CW lamp does not fire on
the first try.
Turn the power off and back
on again. Try 3 or 4 times to
fire the lamp.
If unsuccessful after 3 or 4
tries, contact the factory for
replacement.
Signal-to-Noise level (900:1
on Raman band of water)
drops off.
The CW lamp is oscillating.
Call the factory for a
replacement.
No signal is seen.
Check that the sample
holder lid is on tight,
activating the interlock
switch.
If signal resumes, switch has
been activated.
No signal is seen.
Interlock switch could be
stuck. Tap it with your finger
to see if it pops up at your
touch.
If signal does not reappear,
switch may be stuck. Call
factory for assistance.
28
Figure 13 Normal Time Trace
Figure 14 Time Trace with Lamp Oscillation
29
Hardware Maintenance Procedures
Procedure 1.
Removal and Replacement of the Top Cover
REQUIRED TOOLS:
•
Flat-tip screwdriver.
REMOVAL
WARNING:
1.
2.
USE EXTREME CAUTION IF YOU MUST REMOVE THE TOP COVER WHILE
POWER IS APPLIED TO THE INSTRUMENT.
Refer to the Front View drawing. Firmly grasp the top cover on each end of the unit. Newer
instruments have a screw on the left side. Remove it now if it exists.
Lift the cover straight up and off of the instrument.
REPLACEMENT
1.
Refer to the drawings labeled Front View, Right Side View, and Left Side View.
1.
Note the locations of the slots on the baseplate.
2.
Align the edges of the top cover with the slots on the sample chamber and power panel.
3.
Slip the cover into the slots, and slide the cover straight down onto the instrument. Replace
the screw if it was removed earlier.
30
Slot
Slot
AMINCO-BOWMAN Series 2
Luminescence Spectrometer
Sample Compartment
Figure 15 Front View
SLOT
RIGHT CHANNEL
PMT SOCKET
DIAGNOSTIC/RESET
SWITCH W/LED
TTL IN
AUX 1
TTL OUT
PMT
MONITOR
Figure 16 Right Side View
SLOT
VENT
SLOTS
ON/OFF
POWER
PLUG
FUSE
PANEL
Figure 17 Left Side View
31
AUX 2
VENT
SLOTS
Procedure 2. Monochromator maintenance procedures
Checking slit calibration
CAUTION:
1.
Be sure to remove the dowel pin from the slit calibration hole before sending the
slit calibration command.
To check the slit calibration, slide an 1/8” diameter dowel pin in the calibration hole at either
end of the monochromator assembly. See Figure 18 Slit Calibration Hole Location.
If you are able to insert the pin, the slit is calibrated; if not you need to perform calibration.
2.
Open the AB2 software program and access Setup – Instrument Setup – Send Commands.
For Instrument Control String, type: EX:SLITCAL or EM:SLITCAL and press <SEND>.
You should hear an audible response from the monochromator being checked. If the screen
returns an error message at this point, it is probably an indication that the mono slit wheel is
malfunctioning. If this is the case, the monochromator must be exchanged or repaired.
3.
Test for calibration by inserting the 1/8” diameter dowel pin through the slit calibration hole
at the end of the monochromator. If the slit is STILL not calibrated, turn to the
Troubleshooting Guide.
HOLE FOR
CALIBRATION
PIN TYP BOTH
ENDS
Figure 18 Slit Calibration Hole Location
Removal and replacement of monochromators
Refer to Figure 19 Monochromator Baseplate Connections. This block diagram shows the
general location of the mounting screws for each monochromator.
Removal and replacement of excitation monochromator
REQUIRED TOOLS:
• 9/64 ball-point hex driver
• Small Phillips screwdriver
• Hex driver handle with extension
32
•
Straight tip screwdriver
REMOVAL:
1.
Shut off power to the instrument and unplug the power able from the left hand side of the
unit.
2.
Remove the top cover from the instrument (refer to the Removal and Replacement of the
Top Cover procedure in the Hardware Maintenance Procedures chapter).
3.
Put the 9/64 ballpoint hex driver in the hex driver extension handle.
4.
Refer to Figure 19 Monochromator Baseplate Connections below. This block diagram
shows the general location of the mounting screws and interface connector cable for the
excitation monochromator.
5.
Use the 9/64 ballpoint hex driver to remove the three mounting screws.
6.
Use the straight tip screwdriver to loosen the EX mono interface cable connector.
7.
Remove the interface cable from the baseplate.
8.
Refer to Figure 51 Light Tube Assembly - Sheet 1. Use the small Phillips screwdriver to
remove the two screws (#36) that secure the light baffle (#37) to the lamp assembly.
CAUTION:
9.
10.
DO NOT TOUCH THE LAMP LENS WITH YOUR FINGERS. USE
EXTREME CARE THAT YOU DO NOT SCRATCH THE LENS WITH
ANY TOOLS.
Lift the lamp sensor-mounting bracket off of the two mounting screws at the left end of the
monochromator.
Slightly lift the right end of the mono up while pulling it towards the left side of the baseplate.
At this point the pin on the end of the shutter should disengage from the shutter control arm.
Continue to lift the mono up and out of the baseplate.
CAUTION:
DO NOT FORCE THE SHUTTER FREE BY BENDING THE SHUTTER
CONTROL ARM, WHICH WILL CAUSE THE SOLENOID TO
MALFUNCTION.
11.
Slide the shutter out of its guide assembly.
12.
Remove the guide assembly by removing the four (4) screws that secure it to the left end of
the monochromator.
33
CARD CAGE
MOUNTING SCREWS
EX
MONO
POWER
SUPPLY
EX
EM
EM
MONO
SAMPLE
CHAMBER
MOUNTING SCREWS
Figure 19 Monochromator Baseplate Connections
REPLACEMENT:
1.
Install the shutter guide assembly on the left end of the monochromator.
2.
Use graphite, see Figure 1 Shutter Lubrication Points, or pencil lead to lubricate the top and
bottom edges of the shutter.
3.
Slide the shutter into the guide assembly. Ensure it moves freely in the guide assembly.
4.
Slide the monochromator down and to the right in its baseplate recess. Ensure that the
shutter pin is aligned with the shutter control arm.
5.
Ensure that the dowel pins on the mounting brackets align with the baseplate holes.
6.
Install the three mounting screws to secure the monochromator.
7.
Re-attach the EX mono interface cable to its connector on the baseplate.
8.
Ensure that the shutter control arm and shutter move freely. Adjust the solenoid and control
arm as needed to ensure proper operation.
9.
Place the lamp sensor bracket back on its two mounting screws at the left end of the mono.
10.
Reinstall the light baffle on the lamp assembly.
11.
If this is a new monochromator being installed, you will need to enter the new calibration
values on the CPU board.
34
Removal and replacement of emission monochromator
REQUIRED TOOLS:
•
•
•
•
•
9/64 ball-point hex driver
#21 Hex driver
Hex driver handle with extension
Straight tip screwdriver
24” x 24” sheet of black cloth
REMOVAL:
1.
Shut off power to the instrument and unplug the power cable from the left hand side of the
unit.
2.
Remove the top cover from the instrument (refer to the Removal and Replacement of the
Top Cover procedure in the Hardware Maintenance Procedures chapter).
3.
Put the 9/64 ballpoint hex driver in the hex driver extension handle.
4.
Refer to Figure 19 Monochromator Baseplate Connections. This block diagram shows the
general location of the mounting screws and interface connector cable for the emission
monochromator.
5.
Use the 9/64 ballpoint hex driver to remove the three mounting screws.
6.
Use the straight tip screwdriver to loosen the EM mono interface cable connector.
7.
Remove the interface cable from the baseplate.
8.
Refer to Figure 20 Shutter Cover and Lens Tube Position below. Use the #21 hex driver to
remove screw #2, and the 9/64 ballpoint hex driver to remove screw #1 from the shutter
cover. Take note that screw #1 also holds a support brace in place beneath the baseplate.
9.
Etch a mark on top of the emission beam tube next to the focusing lens retaining screw to
identify its position. (This step will not be performed if a filter/polarizer wheel is installed.)
13.
Remove the lens retaining screw and slide the lens tube and lens to the right as far as
possible. Be careful NOT to damage the lens. (This step will not be performed if a
filter/polarizer wheel is installed.)
14.
Slightly lift the right end of the mono up while pulling it towards the left side of the baseplate.
At this point, the pin on the end of the shutter should disengage from the shutter control
arm. Continue to lift the mono up and out of the baseplate.
CAUTION:
15.
DO NOT FORCE THE SHUTTER FREE BY BENDING THE SHUTTER
CONTROL ARM. THIS COULD DISFIGURE THE CONTROL ARM, OR
CAUSE THE SOLENOID TO MALFUNCTION.
Use a 9/64 hex driver to remove the four recessed screws that secure the PMT housing to
the end of the EM mono.
35
CAUTION:
PROTECT THE PMT FROM ROOM LIGHT. USE THE 24” x 24” SHEET OF
BLACK CLOTH TO WRAP THE PMT HOUSING IMMEDIATELY AFTER
REMOVING IT FROM THE MONOCHROMATOR.
16. Put the wrapped PMT housing in a safe location where it will not be accidentally dropped.
17. Slide the shutter out of its guide assembly.
18. Remove the guide assembly by removing the four (4) screws that secure it to the left end of
the monochromator.
FOCUSING LENS
RETAINING
SCREW
SCREW #2
SHUTTER
COVER
SCREW #1
EM
MONO
LENS TUBE
SAMPLE
CHAMBER
BASEPLATE
SUPPORT
BRACE
Figure 20 Shutter Cover and Lens Tube Position
REPLACEMENT:
1.
Install the shutter guide assembly on the left end of the monochromator.
2.
Use graphite, see Figure 1 Shutter Lubrication Points, or pencil lead to lubricate the top and
bottom edges of the shutter.
3.
Slide the shutter into the guide assembly. Ensure it moves freely in the guide assembly.
4.
Carefully unwrap the PMT housing without exposing the PMT to light and attach it to the left
end of the monochromator.
5.
Slide the monochromator down and to the right in its baseplate recess. Ensure that the
shutter pin is aligned with the shutter control arm.
6.
Ensure that the dowel pins on the mounting brackets align with the baseplate holes.
7.
Install the three mounting screws to secure the monochromator.
8.
Ensure that the shutter control arm and shutter move freely. Adjust the solenoid and control
arm as needed to ensure proper operation.
36
9.
Reinstall the shutter cover with its two screws. Ensure that the bottom screw is aligned with
the support brace located underneath the baseplate.
10.
Slide the lens tube back into position. Be sure that it fits snugly inside the snout on the right
end of the monochromator and the left snout on the sample compartment.
11.
Reposition the focusing lens and install its retaining screw. It may be necessary to use the
end of a ball driver or screwdriver to slide the lens back into place. Do this gently and do
NOT scratch the lens surface. DO NOT USE YOUR FINGER TO SLIDE THE LENS INTO
PLACE. Skin oil on the lens will deteriorate the emission signal.
12.
If this is a new monochromator being installed, you will need to enter the new calibration
values on the CPU board. (See Entering Calibration Values on the CPU Board.)
Check monochromator calibration
The purpose of this procedure is to ensure that the monochromators are both calibrated for
proper machine operation.
The first part of this procedure checks for a standard spectrum, called a Raman line, with a peak
appearing at a specific point.
The second part of this procedure checks the emission monochromator, using a mercury peak
from a fluorescent light bulb and the excitation monochromator by using a scatter solution.
NOTE:
Part two of this procedure does not need to be performed if the results of part one
are satisfactory.
REQUIRED EQUIPMENT:
For part one of this procedure, a clean quartz cuvette of pure distilled water will be needed. It is
extremely important that the cuvette is thoroughly clean and the distilled water is as free of
contaminants as possible.
For part two, a clean quartz cuvette with a mixture of distilled water and powdered milk will be
needed. A small 3/32” straight-tipped screwdriver will also be needed to change the calibration
settings on the CPU board. A desktop fluorescent lamp will also be needed.
PART ONE:
1.
Ensure the lamp enable switch is on.
2.
Turn power on to the instrument.
3.
Turn on power to the computer.
4.
Select and run the AB2 software.
5.
Place a clean quartz cuvette of distilled water in the sample chamber.
6.
Select Setup from the menu bar.
7.
Choose Instrument Setup from the drop down menu.
37
8.
Choose Monochromators from the Setup window.
9.
Ensure that the excitation monochromator is set to 350nm.
10.
Ensure that the emission monochromator is set to 397nm.
11.
Set the emission step size to 0.2nm.
12.
Set both bandpass settings to 4nm.
13.
Open both shutters.
14.
Close the Monochromator window by clicking on the OK button.
15.
Select Sensitivity from the Setup window.
16.
Select Auto-range and wait for the software to set the high PMT voltage.
NOTE:
At this point, there should be a reading of approximately 60% on the EM channel.
The actual amount of high voltage that must be applied to achieve this signal
level may vary depending on the age and condition of the xenon lamp.
17.
Use the mouse to move the Sensitivity window to an easily accessible section of the screen.
18.
Select Applications from the menu bar.
19.
Choose Emission Wavelength Scan from the drop down menu.
20.
Set the lower limit to 370nm.
21.
Set the upper limit to 430nm.
22.
Set the scan rate to 1nm/sec.
23.
Set the repetition to 1.
24.
Click the mouse pointer on the OK button.
25.
Select Applications from the menu bar.
26.
Choose Start Application from the drop down menu.
27.
Change the file name from SPEC0001 to RAMAN1.
28.
Click the mouse pointer on the Start button.
NOTE:
At this point, the instrument should be performing an emission scan.
29.
The screen should show a data window similar to that of Ramen Band of Distilled Water,
diagram when the scan has completed.
30.
Select View from the data window menu bar.
31.
Select Cursor from the drop down menu.
38
32.
Move the mouse pointer to the Display Cursor box and click. A check should appear in the
box.
33.
A crosshair cursor should now be visible inside the data window. The position of the vertical
line is the one we will be concentrating on.
34.
Move the mouse pointer to the highest point of the Raman spectrum and click. The cursor
should move to the peak.
35.
Note the X and Y values displayed in the upper right corner of the data window.
36.
Use the left and right arrow keys of the keyboard to locate the highest Y value that can be
attained on the spectrum. The corresponding X value, at this point, should be 397nm (plus
or minus 1.0nm).
37.
If the peak of the Raman line occurs, as stated in step 34, the monochromators are within
calibration and no other action is required. If the peak does NOT occur within these limits,
you must perform part two of the mono calibration procedures.
39
397nm
FLUORESCENCE
10
0
370
430
EMISSION WAVELENGTH
Figure 21 Ramen Band of Distilled Water
AIR FLOW
TOP VIEW
½ INCH
CLEARANCE
FOR HV
AIR FLOW
END VIEW
Figure 22 Forced Air Cooling
Figure 23 Lamp Fan Power Cable
40
F1
6A,
250V
HV
TERMINAL
#14
DO NOT
ATTACH OR
REMOVE CURRENT METER
WHILE AC POWER IS
APPLIED TO UNIT
#14
Figure 24 Checking Lamp Current
PIN 4
PIN 1
Figure 25 Mating Connector for J1
41
R31, RANGE
FACTORY ADJUST
R9, MOD MAX
R15, MOD MIN
R27, CURR
Figure 26 Adjustment Locations
A1E13
-HV
E2, AC INPUT
E1, AC INPUT
4
3
E3, POWER GROUND
2
1
J1
E12
+ OUTPUT
Figure 27 Input, Output and Control Connections
42
EARTH GROUND
E3 GROUND
250
115
+ OUT PUT
E12
ANODE
E1 AC
230
LAMP
210
CATHODE
E2 AC
230 VAC
- HV
POWER SWITCH and FUSE
POWER TRANSFORMER
POWER SUPPLY
Figure 28 Power Transformer Connection
PART TWO:
1.
Remove the top cover from the instrument.
2.
Check the calibration rotary switch settings on the upper left corner of the CPU board.
These switches should be set to the factory-recorded values for each monochromator.
Figure 30 Sample Calibration Setting on the Motherboard, shows an example of these
rotary switch settings. A 3/32” flat-tipped screwdriver is needed to change the switch
settings. To verify the rotary switch settings, check for one of the following:
a.
ORIGINAL MONOCHROMATORS – The calibration points are recorded on an
identification label attached on the lower rear panel of the instrument.
b.
NEW MONOCHROMATORS – If the monochromators are being installed as
replacement parts, they will have their own factory-set calibration values. The
calibration value for a new monochromator will be supplied with the mono. Enter the
new calibration value on the CPU board rotary switches using a small flat-tipped
screwdriver.
3.
Be sure the Lamp Enable switch is on.
4.
Power on the instrument and the computer.
5.
Initialize the instrument software, if it is not currently running.
6.
Select Setup from the menu bar.
7.
Choose Instrument Setup from the drop down menu.
8.
Choose Monochromators from the Setup window.
9.
First check the emission monochromator to see how close it is to its calibration value. Set
the following parameters in the Monochromators window:
•
•
•
EM shutter:
EM step size:
EM wavelength:
OPEN
0.2nm
435.8nm
43
•
EM bandpass:
1nm
10.
Remove the knob from the sample compartment lid.
11.
Prepare a diluted scatter solution of powdered milk (coffee creamer) and distilled water.
NOTE:
Glycogen, if available, can be used in place of powdered milk.
12.
Place the mixture in the sample compartment.
13.
Place the sample compartment lid over the sample compartment. Be sure that it is fully
seated and that the micro switch, located on the rear wall of the sample chamber, is
pressed down.
14.
Place a desktop fluorescent lamp so that it shines down through the hole on top of the
sample compartment lid.
15.
Leave the Monochromator Setup window displayed on the screen.
16.
Also, choose Sensitivity from the Setup window.
17.
Adjust the windows with the mouse so you can see the Setup, Monochromator and
Sensitivity windows simultaneously on the screen.
18.
Set the PMT high voltage to 700 volts in the Sensitivity window.
19.
Open both monochromator shutters in the Monochromators window.
NOTE:
Lower the PMT high voltage value if the EM data reading indicator turns red.
20.
Move the mouse pointer to the main menu window and select Applications from the menu
bar.
21.
Select Emission Wavelength Scan from the drop down menu.
22.
Set the scan limits as follows:
•
•
•
•
LOWER LIMIT:
UPPER LIMIT:
RESOLUTION:
REPETITIONS:
430
440
0.2
1
23.
Select OK to accept the values entered.
24.
Select Applications from the spectrometer window menu bar.
25.
Select Start Applications from the drop down menu.
26.
Enter EMHGPK1 as the name in the “Data will be saved as” field.
27.
Select OK to begin the acquisition.
28.
The instrument should begin running an emission scan.
29.
Select View from EMHGPK1 data window menu bar.
44
30.
Select Display cursor from the drop down menu.
31.
Move the cursor to the peak of the spectrum showing in the EMHGPK1 data window.
32.
The peak should occur at 435.80nm (plus or minus 1.0nm).
33.
If the peak does not occur within the limits stated in step 32, perform the following steps:
a.
Change the EM calibration rotary switch settings on the CPU board.
NOTE:
When the rotary switch settings are increased, the spectrum will shift
downward. If the rotary switch settings are decreased, the spectrum will shift
upward.
As an example: If the original EM switch settings were 03.8 and the peak of
the spectrum occurred at 435.6nm, changing the EM switches to 03.6 causes
the peak to occur at 435.4nm. On the other hand, if the switches were
changed to 04.0, the peak would occur at 435.8nm.
b.
Select Send Commands from the Setup window.
c.
Enter EM:CAL as the Instrument Control String.
d.
Repeat steps 24 through 33, but change the name of each data acquisition to reflect its
sequence (i.e. EMHGPK2, EMHGPK3, EMHG4, etc.). Repeat these steps until the
emission monochromator peak occurs at 435.8nm (plus or minus 0.6nm).
34.
Reattach the knob to the sample compartment lid.
35.
Next check the calibration of the excitation monochromator.
CAUTION:
36.
MONITOR THE EM DATA SIGNAL IN THE SETUP WINDOW CLOSELY,
AS YOU PERFORM THE NEXT STEP. IMMEDIATELY SHUT OFF THE
PMT HIGH VOLTAGE IN THE SENSITIVITY WINDOW, IF THE SIGNAL
GOES INTO THE RED ZONE.
Move the mouse pointer to the Monochromators window and set the following parameters:
•
•
•
•
EM shutter:
EX step size:
EX wavelength:
EX bandpass:
OPEN
0.2nm
350nm
1nm
•
•
•
•
EM shutter:
EM step size:
EM wavelength:
EM bandpass:
OPEN
0.2nm
350nm
1nm
37.
Set the PMT high voltage to 250 volts in the Sensitivity window.
38.
Slowly increase the high voltage to achieve a data signal of 5.00.
NOTE:
Lower the PMT high voltage value if the EM data reading indicator turns red.
45
39.
Move the mouse pointer to the main menu window and select Applications from the menu
bar.
40.
Select Excitation Wavelength Scan from the drop down menu.
41.
Set the scan limits as follows:
•
•
•
•
LOWER LIMIT:
UPPER LIMIT:
RESOLUTION:
REPETITIONS:
345
355
0.2
1
42.
Select OK to accept the values entered.
43.
Select Applications from the menu bar.
44.
Select Start Applications from the drop down menu.
45.
Enter EXSCATR1 as the name in the “Data will be saved as” field.
46.
Select OK to begin the acquisition.
47.
The instrument should begin running an excitation scan.
48.
Select View from EXSCATR1 data window menu bar.
49.
Select Display cursor from the drop down menu.
50.
Move the cursor to the peak of the spectrum showing in the EXSCATR1 data window.
51.
The peak should occur at 350.0nm (plus or minus 1.0nm).
52.
If the peak does not occur within the limits stated in step 51, perform the following steps:
a.
Change the EX calibration rotary switch settings on the CPU board.
NOTE:
When the rotary switch settings are increased, the spectrum will shift downward.
If the rotary switch settings are decreased, the spectrum will shift upward.
As an example: If the original EX switch settings were 07.0 and the peak of the
spectrum occurred at 349.8nm, changing the EX switches to 07.2 causes the
peak to occur at 349.6nm. On the other hand, if the switches were changed o
06.8, the peak would occur at 350.0nm.
53.
b.
Select Send Commands from the Setup window.
c.
Enter EX:CAL as the Instrument Control String.
d.
Repeat steps 43 through 52 but change the name of each data acquisition to reflect its
sequence (i.e. EXSCATR2, EXSCATR3, EXSCATR4, etc.). Repeat these steps until
the excitation monochromator peak occurs at 350.0nm (plus or minus 0.6nm).
Repeat part one of the calibration procedures to ensure that the Raman line of water occurs
within the specified limits.
46
Procedure 3.
Removal and replacement of circuit boards
To remove any of the circuit boards, the following general information applies:
1.
Shutoff power to the instrument.
2.
Remove the top cover of the instrument.
3.
Lift up on the white plastic tabs on each side of the circuit board.
4.
When the board disengages, lift it straight up and out of the instrument.
To replace a board:
1.
Ensure that the component side of the board is facing forward.
2.
Align the edges of the circuit board with the corresponding slots on the card cage assembly.
3.
Slide the card straight down into the card cage.
4.
Apply only enough pressure to snap the board into position.
5.
Attach the interface ribbon cable (CPU board only).
Refer Figure 37 PCB Card Cage Sheet 1, for specific circuit board locations. Each board has its
own unique position in the card cage.
The Filter Wheel board is located in the left rear position of the card cage and plugs into
connector P6. This is an optional board and will only be installed if your instrument is equipped
with the Filter Wheel assemblies.
The high voltage and mono board is located in the right rear position of the card cage and plugs
into P4 and P5.
The CPU processing board is located in the front of the card cage and plugs into connectors P1,
P2 and P3. If you remove this board, note the location and orientation of the ribbon cable
attached to the front of the board.
NOTE:
Procedure 4.
PARTS:
If the CPU board is exchanged, be sure to change the EM and EX
monochromator calibration rotary switches on the new board to the same settings
that are on the old board (see Entering Calibration Values on the CPU Board)
Installing the Lens Spacer between the Emission Monochromator and the
Sample Holder
Lens Retaining Ring, Lens, Spacer, and Screw
When the system does not come equipped with the Filter Wheel assembly (SQ-320), the factory
installs an empty lens tube. The lens tube helps block out any outside light and acts like a spacer
or a light buffer.
47
LENS
LENS TUBE
LENS SPACER
LIGHT TUBE
ADAPTER
EMISSION
BEAM TUBE
LEFT CHAMBER
PLATE
Figure 29 Emission Monochromator and Emission Lens Tube
Procedure 5.
Entering calibration values on the CPU board
REQUIRED EQUIPMENT:
• 3/32” flat-tipped screwdriver
1.
Insert and turn the screwdriver tip in the slot of the rotary switch that you wish to change.
The first three red rotary dipswitches are those for the excitation monochromator. Consult
the front of your operator’s manual, the data label on lower rear panel of the instrument, or
on the monochromator for the calibration points set at the factory.
Dial in the calibration points (e.g.,, -32.8. All calibration points will be negative).
2.
The three dipswitches on the right are for the emission monochromator (e.g.,, -60.1).
48
1
1
0
0
1
1
0
0
7 8 9
7 8 9
1
6
0
5
6
7 8 9
7 8 9
EX MONO
2 3 4
5
6
6
1
2 3 4
5
5
6
6
5
5
0
2 3 4
2 3 4
7 8 9
2 3 4
7 8 9
2 3 4
EM MONO
Figure 30 Sample Calibration Setting on the Motherboard
Procedure 6.
Installing the GPIB cable
1.
Attach one end of the GPIB cable to the I/O Panel GPIB connector located on the side of
the instrument. Refer to Figure 6 I/O Panel - Instrument Right Side
2.
Attach the other end of the GPIB cable to the GPIB interface adapter port on the back of the
computer.
3.
Ensure that the Lamp Enable is ON and power up the instrument. The lamp sensor indicates
that the lamp is on. Watch for the RESET light to turn green. See diagram of I/O Panel.
4.
In the software program, access Setup – Instrument Setup – Monochromators.
5.
Listen for the instrument to respond.
6.
Click the Open Shutter and Close Shutter buttons open and closed.
7.
Set the excitation monochromator to 575nm.
8.
Check for yellow light emitting from the excitation monochromator by holding a white card in
the light path.
Procedure 7.
CAUTION:
Removal and Replacement of the Photomultiplier Tube FA-277 (R2949)
KEEP THE PMT PROTECTED FROM ROOM LIGHT AT ALL TIMES. ANY
TIME THE PMT IS REMOVED FROM HOUSING, SHUT OFF THE ROOM
LIGHTS OR COVER THE PMT FACE WITH A BLACK CLOTH. EXCESSIVE
LIGHT OF ANY KIND CAN DAMAGE THE PMT.
REMOVAL:
REQUIRED EQUIPMENT:
• 8/32 Allen head hex driver
• Sheet of black cloth
1.
Shut off power to the instrument.
2.
Remove the top cover of the instrument.
3.
Unplug the high voltage and signal cables from their baseplate connectors.
49
4.
Refer to Figure 31 Photomultiplier Housing and Socket. Remove the 8/32 screw that
secures the PMT socket to the housing.
5.
Firmly grasp the base of the PMT socket. Twist and pull it until it comes free from the
housing.
6.
Cover the PMT with the black cloth and disconnect it from the socket assembly.
7.
Store the tube in a safe place, if it is to be reused.
8.
If the tube is unserviceable, place it in the box that the replacement tube came packaged in.
Then discard the old tube.
REPLACEMENT:
1.
Align the key on the base of the PMT with the notch on the socket. Firmly push the PMT
into the socket until it is fully seated.
2.
Align the PMT socket with the housing, as shown in Figure 31 Photomultiplier Housing and
Socket.
3.
Twist the socket until both o-rings go in and the screw fits into the slot. The slot and the
screw must be aligned.
4.
Tighten the screw until snug. DO NOT OVERTIGHTEN.
NOTE:
PMT SOCKET
The slot and the screw must be aligned.
PMT
HOUSING
Figure 31 Photomultiplier Housing and Socket
Optimize the PMT output signal
1.
Turn on power to the instrument.
2.
Power up the computer and access the AB2 software.
3.
Select Setup from the menu bar.
4.
Select Instrument Setup from the drop down menu.
5.
Select Monochromators from the Setup window. Open both shutters and place both
monochromators to 350nm.
50
6.
Also select Sensitivity from the Setup window. Ensure that the PMT high voltage if off.
7.
Attach a digital multi-meter to the PMT MONITOR connector shown on the Figure 6 I/O
Panel - Instrument Right Side to monitor the PMT signal as it changes.
NOTE:
The PMT signal can also be monitored by watching the EM Data Signal in the
Setup window.
8.
Prepare a dilute solution of powdered milk and distilled water and place it in the sample
compartment.
9.
Adjust the PMT high voltage to approximately 500 volts in the Sensitivity window.
NOTE:
Monitor the EM Data Signal in the Setup window very closely to ensure that the
PMT does not go into saturation (color bar turns RED). If the color bar goes into
the RED region, immediately lower the PMT high voltage. If this does not reduce
the signal, reduce the EM mono Bandpass setting in the Monochromators
window.
10.
Loosen, but do not remove, the 8/32 screw that secures the PMT socket in the housing
11.
Monitor the EM Data Signal and/or the digital multi-meter reading as you twist the PMT
socket in the housing. You will be watching for the signal to reach a maximum value. When
you achieve the maximum, tighten the 8/32 screw on the PMT.
Procedure 8.
Installing Filter Wheels
Emission Filter Wheel installation:
1.
Turn off power to the instrument.
2.
Remove the top cover of the instrument.
3.
Locate the emission lens focusing adjustment screw on top of the emission beam tube. See
the Emission Monochromator and Emission Lens Tube diagram.
4.
Etch a mark on top of the beam tube, next to the focusing adjustment screw. This will serve
as an alignment reference after the Filter Wheel assembly has been installed.
5.
Loosen the lens focusing adjustment screw. Slide the screw as far to the right as it will go.
6.
A setscrew secures the lens spacer to the emission monochromator. Loosen the setscrew
and slide the spacer as far to the right as it will go.
7.
Remove the four screws inside the sample chamber that hold the emission beam tube in
place.
8.
Remove the focusing adjustment screw from the top of the beam tube.
9.
Continue to slide the lens spacer to the right until it is clear of end of the emission
monochromator.
NOTE:
Do not push the focusing lens out of the emission beam tube.
51
10.
Remove the lens spacer. Ask the customer where he might want to store it. The lens spacer
will be needed if the emission Filter Wheel is ever removed.
11.
Reinstall the emission beam tube on the side of the sample chamber.
12.
Use a dull instrument, such as the end of a pencil or pen, to slide the focusing lens back to
the left, inside the beam tube.
13.
Align the adjustment screw with the mark you made in Step 4. Tighten the screw finger
tight.
14.
Slip the Filter Wheel assembly into place between the emission monochromator and the
emission beam tube.
15.
Align two pins on the bottom of the Filter Wheel with the two mounting holes in the
baseplate.
16.
Anchor the Filter Wheel with its two mounting screws.
17.
Attach the interface cable to the baseplate.
Excitation Filter Wheel installation:
1.
Shutoff power to the instrument.
2.
Remove the top cover of the instrument.
3.
Position the Filter Wheel assembly between the excitation monochromator and the beam
splitter assembly.
4.
Align the pins on the bottom of the Filter Wheel with the mounting holes in the baseplate.
5.
Anchor the Filter Wheel with its two mounting screws.
6.
Attach the interface cable to the baseplate.
7.
Check the Filter Wheel operation.
Checking Filter Wheel operation:
1.
Turn on power to the instrument.
2.
Turn on the computer.
3.
Start the AB2 software.
4.
Select SETUP from the menu bar.
5.
Select INSTRUMENT SETUP from the drop down menu.
6.
Select ACCESSORIES from the Instrument Setup window.
7.
Click the mouse pointer on each of the selections for the emission monochromator. Verify
that the emission Filter Wheel rotates to each position.
52
8.
Click the mouse pointer on each of the selections for the excitation monochromator. Verify
that the excitation Filter Wheel rotates to each position.
9.
Place the top cover on the instrument.
Procedure 9.
Checking Filter Holder placement and optical alignment
Filter Holder placement is extremely crucial to the angle that the light beam travels into and out of
the sample chamber. The filter holders are located inside the sample chamber. One is mounted
on the rear wall. The other is mounted on the left wall.
There is a focusing lens in each holder. If either of the two holders are tilted or removed from the
sample chamber, the throughput of the signal will diminish.
Under normal operating conditions, a Raman scan of distilled water is a sufficient check to ensure
that throughput of the system is acceptable. A Raman line of water signifies several aspects of
instrument operation. It verifies that the lamp output is adequate. It also verifies that the
monochromators are both aligned properly. It can be an indicator that the PMT is operating
normally. It can also be an excellent check to verify that the system’s acquisition electronics are
working normally.
The xenon lamp is usually the first component to suspect if the emission signal begins to fall to
unacceptable levels. A failing lamp, however, will usually cause sporadic signal level changes.
You will be able to physically see the intensity level of the lamp change. Replacing the lamp is the
only cure at that point.
However, if the system’s throughput diminishes (usually for no apparent reason), it could be an
indication that a component within the optical path of the light beam is out of alignment.
Filter Holder placement:
1.
Connect a digital voltmeter to the PMT MONITOR connector on the I/O panel.
2.
Place a clean quartz cuvette of distilled water in the sample compartment.
3.
Setup the instrument as outlined in Part One, Steps 1 through 16 of the Monochromator
calibration procedures.
4.
You should see a voltage reading of approximately 8.00 on the DVM when the PMT high
voltage is set between 750 and 900 volts.
Aligning the beam focusing mirror:
NOTE:
Use a 23-hex ball driver for this procedure.
Perform this procedure if the image is off center or no signal is coming through the system.
1.
Set up the system first with the following standard defaults:
•
•
•
•
•
Excitation Wavelength:
Emission Wavelength:
Bandpass:
High Voltage:
EX and EM Shutters:
350
397
4
820
Open
53
2.
First power down then turn the power ON to fire up the Continuous Wave Lamp.
3.
There are 3 screws on the back of the mirror mount. Loosen the middle one (B). Adjust the
top screw (A) in and out for maximum signal on the voltmeter or follow the procedure in
Step 5. Lock the middle (B) screw down when the mirror is in the right position (see
Figure 32 Alignment of the Mirror).
4.
In order to achieve a maximum peak with a voltmeter; loosen the emission lens screw in
and out while checking for an increase on the voltmeter. See the Emission Monochromator
and Emission Lens Tube diagram.
5.
In the software program, access:
•
•
•
•
Setup
Setup Instrument
Channel
Readings Full Scale 0% - 100%
o
o
Loosen the middle screw (B).
While keeping an eye on the screen, adjust the top screw (A) until Voltage
Reading is at a maximum peak.
Lock in the highest possible reading with the middle screw (B).
Loosen the bottom screw (C).
o
o
54
DO NOT
ADJUST THIS
SCREW
A
B
3 ADJUSTMENT
SCREWS
C
Figure 32 Alignment of the Mirror
Procedure 10. Removal and replacement of the Motherboard Assembly
NOTE:
THE MOTHERBOARD IS NOT A FIELD REPLACEABLE ITEM. If the
motherboard needs to be replaced, the entire unit must be returned to distributor
for replacement and testing.
Procedure 11. Removal and Replacement of the Power Supply
REQUIRED TOOLS:
• #2 Phillips screwdriver
Power supply removal:
REFERENCE:
ECO
ACTION:
The SQ-302 and the SQ-309 Power Supplies have been terminated and are
replaced with the SQ-390S and SQ-395S, respectively
PRODUCT
AFFECTED:
All models with serial number ≥ xxxE084001 have been manufactured using the new
power supply buckets. {NOTE: S/N 2B9E108001 is an exception – was
manufactured using the older power supply bucket.}
APPLIES TO:
71839 and 72025
Repair at customer site
55
Repair at Depot
Effective immediately:
SQ-302 and SQ-309 have been terminated as service parts, and have been
replaced by the following replacement parts.
REPLACEMENT PARTS:
SQ-390S
Power Supply Bucket with CW Supply*
Includes 5-A fuse for 115 V models and 3-A fuse for 250 V models
Used on models with:
CW Lamp only
CW Lamp & Flash Lamp
SQ-395S
Power Supply Bucket without CW Supply
Includes 5-A fuse for 115 V models and 3-A fuse for 250 V models
Used on models with Flash Lamp only.
SQ-394 CW Lamp Power Supply*
CW Lamp Power Supply for the SQ-390S or SQ-395S Power Supply Bucket.
*NOTE: The power supply is set to the correct CW Lamp Current at the factory.
However, if you find that the power supply is out of adjustment, the
instructions for adjusting the CW Lamp Current are appended to the
Bulletin.
Adjust CW Lamp Current:
Connect the Lamp and Current Meter as shown in Figure 33 Power Supply Outlet Connectors.
Move the LAMP ENABLE switch to "ON". Plug in the lamp fan. DO NOT plug in a card cage with a
CPU board; the card cage fan fixture is OK. Plug Lamp Detector/Lamp Monitor cable into the 3-pin
Burndy receptacle. Power up the power supply and perform the following:
1.
Verify that the lamp fan works and the lamp ignites.
2.
Verify that about 3.5 sec after power is applied, the LED on the PCB comes on.
CAUTION:
WHEN PERFORMING THE FOLLOWING STEPS, DO NOT LET THE
LAMP CURRENT EXCEED 14A OR RISK BLOWING THE FUSE ON
THE POWER SUPPLY (SQ-394).
3.
Let the lamp warm up for one minute.
4.
Check the lamp current. If it is between 12.9A and 13.1A, no adjustment is necessary. Go to
Step 6. If adjustment is required, proceed with Step 5.
5.
Try to set the lamp current to 13A by adjusting pot on Power Supply (SQ-394).
6.
Power down the power supply and wait for the capacitors to discharge until the relay "clicks".
56
Figure 1
Figure 33 Power Supply Outlet Connectors
Procedure 12. Removal and replacement of the CW lamp
See Figure 51 Light Tube Assembly - Sheet 1
Cartridge Assembly
WARNING:
TURN THE LAMP ENABLE SWITCH AND COMPUTER OFF BEFORE
BEGINNING THIS PROCEDURE. ALLOW THE LAMP UNIT TO COOL
FOR APPROXIMATELY FIFTEEN (15) MINUTES. TURN OFF POWER
AND UNPLUG. REMOVE THE TOP COVER TO GAIN ACCESS TO
THE LAMP ASSEMBLY.
1.
Remove the two (2) screws securing the SQ-223 PMT Power Supply cover plate, and
remove the plate.
2.
Unscrew the H-0688 screw located in the SQ-239 High Voltage Shield.
3.
Remove the SQ-239 High Voltage Shield.
4.
Unscrew the Phillips head screws that secure the SQ-317 Lamp Power Supply Cables to
the lamp mount.
5.
Remove the SQ-317 Lamp Power Supply Cables from the SQ-194 CW Lamp Mount.
NOTE:
Leave the SQ-317 Lamp Power Supply Cables connected to the power supply.
6.
Squeeze the sides of the E-1302 Con-Plug (at the end of the red-wire and blue-wire
assembly leading from the E-0374 D/C fan) and disconnect from the power supply.
7.
Remove the gold retaining screws (or the Phillips head screws on newer models) that
57
secure the SQ-195 Mount Base Plate of the Lamp Assembly to the base.
8.
Carefully remove the Lamp Assembly from the instrument and stand the Lamp Assembly on
the open end of the SQ-173 Lamp Exhaust Tube. The SQ-196 Mounting Plate will be on
the top.
WARNING:
DO NOT TOUCH ANY LENSES OR GLASS TYPE SURFACES.
9.
Remove the (2) H-0641 screws of the SQ-251 Lamp Baffle.
10.
Remove the SQ-251 Lamp Baffle.
11.
Remove the three (3) H-0730 Phillips flat head screws from the SQ-196 Mounting Plate.
12.
Remove the SQ-196 Mounting Plate. You can now see the lamp easily.
13.
Remove the three (3) M-4025 Standoffs, the SQ-170 Insulator, and the SQ-258 Gasket.
NOTE: There are two (2) more SQ-258 Gaskets that are located between the SQ-197
Lens Mount/Seal and the SQ-170 Insulator. They will probably remain in the
Mounting Plate assembly, but if they fall out, simply reinsert them carefully
without touching the lens.
NOTE: DO NOT ADJUST any of the three (3) H-0109 screws that are in the SQ-197
Lens Mount/Seal. These are critical settings, which must be adjusted only
by service personnel.
14.
Remove the FA-276 Lamp Cartridge Assembly.
15.
Write the Lamp Serial Number, the Date and the Start Hour (from the Hour Meter) on the
SQ-271 Lamp Hours Label located on the SQ-173 Lamp Exhaust Tube.
16.
Insert the new FA-276 Lamp Cartridge Assembly into the SQ-194 CW Lamp Mount. Align
the two (2) screw holes in the FA-275 Lamp Cartridge Assembly with the two holes on the
SQ-194 CW Lamp Mount.
CAUTION:
DO NOT TO TOUCH THE LAMP FACE OR THE CERAMIC SIDE OF
THE LAMP DURING THIS EXCHANGE. OIL FROM YOUR FINGERS
CAN CAUSE THE LAMP TO EXPLODE. IF YOU ACCIDENTALLY
TOUCH THE QUARTZ LENS, REMOVE THE FINGERPRINTS WITH A
CLEAN CLOTH SATURATED WITH PURE ALCOHOL (E.G.,,
METHANOL).
17.
With the lamp assembly still standing upright, replace the SQ-258 Gasket, the SQ-170
Insulator, and the three (3) M-4025 Standoffs onto the Lamp Cartridge Assembly.
18.
Replace the SQ-196 Mounting Plate onto the Insulator and the Standoffs, and secure with
the three (3) H-0730 screws.
19.
Replace the SQ-251 Lamp Baffle onto the Mounting Plate, and secure with the two (2)
H-0641 screws.
20.
Gently position the Lamp Assembly in the instrument until the two (2) H-0039 Dowel Pins in
the SQ-195 Mount Base Plate are inserted into the corresponding holes in the base.
21. Secure the Lamp Assembly to the base using the gold retaining screws (or the Phillips head
58
screws on newer models).
22. Connect the E-1302 connector of the red and blue fan wires to the power supply connector.
(This is keyed so that it can be connected in only one orientation.)
23.
Reinsert the red power supply cable into the larger hole in the side of the SQ-194 CW Lamp
Mount. Reinsert the black power supply cable into the smaller hole in the side of the Lamp
Mount.
24.
Replace the SQ-239 High Voltage Shield on the SQ-194 CW Lamp Mount so the H-0034
Dowel Pin on the Mount is inserted into the corresponding hole in the Shield. Position the
SQ-317 cables so the Shield will fit flush against the Lamp Mount wall.
25.
Secure the Shield to the Lamp Mount wall using the H-0688 screw.
26. Replace the PMT Power Supply cover plate and secure with the two (2) screws.
Figure 34 Lamp Housing
Lamp Housing Assembly
59
WARNING:
TURN THE LAMP ENABLE SWITCH AND COMPUTER OFF BEFORE
BEGINNING THIS PROCEDURE. ALLOW THE LAMP UNIT TO COOL
FOR APPROXIMATELY FIFTEEN (15) MINUTES. TURN OFF POWER
AND UNPLUG. REMOVE THE TOP COVER TO GAIN ACCESS TO
THE LAMP ASSEMBLY.
1.
Remove the two (2) screws securing the SQ-223 PMT Power Supply cover plate, and
remove the plate.
2.
Unscrew the H-0688 screw located in the SQ-239 High Voltage Shield.
3.
Remove the SQ-239 High Voltage Shield.
4.
Unscrew the Phillips head screws that secure the SQ-317 Lamp Power Supply Cables to the
lamp mount.
5.
Remove the SQ-317 Lamp Power Supply Cables from the SQ-194 CW Lamp Mount.
NOTE: Leave the SQ-317 Lamp Power Supply Cables connected to the power supply.
6.
Squeeze the sides of the E-1302 Con-Plug (at the end of the red-wire and blue-wire
assembly leading from the E-0374 D/C fan) and disconnect from the power supply.
7.
Remove the gold retaining screws (or the Phillips head screws on newer models) that secure
the SQ-195 Mount Base Plate of the Lamp Assembly to the base.
8.
Carefully remove the Lamp Assembly from the instrument.
WARNING:
9.
Do not touch any lenses or glass type surfaces.
Gently position the Lamp Assembly in the instrument until the two (2) H-0039 Dowel Pins in
the SQ-195 Mount Base Plate are inserted into the corresponding holes in the base.
10. Secure the Lamp Assembly to the base using the gold retaining screws (or the Phillips head
screws on newer models).
11. Connect the E-1302 connector of the red and blue fan wires to the power supply connector.
(This is keyed so that it can be connected in only one orientation.)
12. Reinsert the red power supply cable into the larger hole in the side of the SQ-194 CW Lamp
Mount. Reinsert the black power supply cable into the smaller hole in the side of the Lamp
Mount.
13. Replace the SQ-239 High Voltage Shield on the SQ-194 CW Lamp Mount so the H-0034
Dowel Pin on the Mount is inserted into the corresponding hole in the Shield. Position the
SQ-317 cables so the Shield will fit flush against the Lamp Mount wall.
14. Secure the Shield to the Lamp Mount wall using the H-0688 screw.
15. Replace the PMT Power Supply cover plate and secure with the two (2) screws.
60
NUT, HEX
SCR SOCKET
HEATSINK
ILC LAMP
SCR PAN
LAMP
XENON
HEATSINK
ANODE
PART OF
LAMP
DO
NO
O
TT
H
UC
COMPOUND
JOINT
CLAMP
HEATSINK
WARNING LABEL
GENTLY PUSH THE
LAMP OUT WITH A
SCREWDRIVER
CW LAMP MOUNT
HEATSINK
CATHODE
!
HEATSINK CLAMP
CONTACT FINGERS
INSTALLING THE CONTINUOUS WAVE LAMP
BRASS
CLIP
PULL OUT LAMP
ASSEMBLY HERE
REMOVING THE CONTINUOUS WAVE LAMP
Figure 35 CW Lamp Assembly
Procedure 13. CW Lamp hard start
PROBLEM:
The CW lamp arcs several times before igniting, or the lamp goes out while it is on.
CAUSES:
1.
2.
3.
4.
5.
6.
7.
Lamp is nearing the end of its usable life.
ILC power supply power output needs to be adjusted to 12.5 amps for lamps using
two heat sink fins or 13.0 amps for lamps using three heat sink fins.
ILC power supply is faulty.
Cable set is arcing between the cables and the chassis.
Faulty electrical connections.
Lamp house needs to be upgraded.
Defective SQ-322 - fan not operating, lamp shuts off after 30 minutes to three hours.
SOLUTIONS:
The following steps address the solution for each of the six situations.
CAUTIONS:
1.
TURN OFF INSTRUMENT POWER, DISCONNECT THE POWER CORD FROM
THE UNIT, AND LET THE CW LAMP COOL DOWN PRIOR TO PERFORMING
MAINTENANCE ON THE INSTRUMENT.
2.
WEAR SAFETY GOGGLES AND PROTECTIVE CLOTHING ANY TIME
MAINTENANCE IS PERFORMED ON THE CW LAMP AND LAMP HOUSING.
3.
DO NOT TOUCH THE SURFACE OR THE CERAMIC SIDES OF THE CW LAMP.
FINGERPRINTS ON EITHER OF THESE SURFACES CAN CAUSE THE LAMP
TO EXPLODE.
4.
HANDLE THE LAMP AND LAMP HOUSING CAREFULLY. DO NOT DROP OR
STRIKE THESE ITEMS AGAINST ANY SURFACE. IMPROPER HANDLING OF
THESE ITEMS CAN CAUSE THE LAMP TO EXPLODE.
61
Step 1: Replacing a faulty lamp
The lamp is meant to be changed as a cartridge. The part number for the lamp cartridge is FA-276 (a
3-heat sink fin component).
Step 2: Adjusting the ILC lamp power supply
In early models, the power supply was adjusted for an output current of seven (7) amps in the simmer
mode. In later models, the current was adjusted to nine (9) amps for the simmer mode. In the most recent
models, the simmer mode has been eliminated altogether. It was found that the simmer mode caused the
lamp electrodes to burn off rapidly. The output current of the ILC lamp power supply is now being
adjusted to 13 amps and the CW lamp is being operated in full power at all times. This process was
necessary due to the fact that the lamps were burning out prematurely.
REQUIRED TOOLS:
•
slotted screwdriver 1/8" tip, 8" shank
•
analog current meter
•
adapter cables
NOTE:
A clamp-on D/C current probe with a multimeter can be used in place of the analog
current meter and adapter cables. A Fluke 80i-1010 current probe, or equivalent, is
recommended. This probe has a +/2(2% + 1 amp) accuracy with a range of 1 to 1000
amps. When used with the multimeter, this probe can be clamped around one of the CW
lamp supply cables while making adjustments to the ILC power supply.
CAUTION: ALLOW THE XENON LAMP TO COOL FOR APPROXIMATELY 30 MINUTES PRIOR
TO PERFORMING THE FOLLOWING PROCEDURES.
Shut off power to the unit.
2.
Remove the top cover.
3.
Unscrew the thumbscrews that secure the lamp house to the baseplate. Reposition the lamp
housing to accommodate removal of the rear cover and to make adjustments to the ILC power
supply.
4.
Remove the rear cover of the power supply bucket.
5.
Locate the R15 (MOD MIN) and R27 (CURR) potentiometers. These potentiometers are located on
the ILC power supply inside the instrument power supply bucket.
NOTE: Number 6 is not necessary if you are using a D/C current probe. Go to step number 7 if
you are using a D/C current probe.
6.
Attach the analog current meter and adapter cables.
7.
Ensure that the LAMP ENABLE switch is in the “ON” position.
8.
Power up the instrument. The lamp should ignite.
9.
Turn potentiometer R15 (MOD MIN) to the full clockwise position. The current should increase to
62
13 amps. If the current does not increase to 13 amps, continue to number 10. If the current does
increase to 13 amps, skip to number 11.
10. Monitor the analog current meter (or multimeter if D/C probe is used) while turning the potentiometer
R27 (CURR). Adjust R27 until the current is 13 amps.
CAUTION:
IF R27 (CURR) IS OVERADJUSTED, FUSE F1 ON THE POWER SUPPLY MAY
BURN OUT.
11. Power down the instrument. Wait approximately 10 seconds for the capacitors to discharge.
12. Disconnect the test and adapter cables.
13. Reconnect the lamp cable to the +OUTPUT connector on the ILC power supply.
14. Place the rear cover on the power supply bucket.
15. Secure the CW lamp assembly to the baseplate with the two thumbscrews. Ensure that the
thumbscrews are tight.
16. Let the lamp cool for approximately 30 minutes.
17. Ignite the lamp and perform the Signal-to-Noise ratio test.
Step 3: Replacing the ILC power supply
The ILC power supply can be changed by replacing the entire power supply bucket (PN: SQ_302), or by
replacing the ILC power supply module (PN: S-0317) within the power supply bucket. If the ILC power
supply module is replaced, it must be adjusted as described in Step 2.
REPLACING POWER SUPPLY BUCKET (PN: SQ-302)
Replace the power supply by following Procedure 11.
REPLACING ILC POWER SUPPLY MODULE (PN: S-0317)
1.
Note that heat sink compound should be applied around the mounting screws.
2.
Adjust and test the power supply module as described in Step 2.
Step 4: Replacing the CW lamp power supply cables
Replace the CW lamp power supply cables if arcing is observed between the cables and the chassis.
Also ensure that the cables are not twisted around each other.
1.
Remove the two thumbscrews that secure the CW lamp assembly to the baseplate.
2.
Remove the rear cover (SQ-155) of the power supply bucket.
3.
Remove the high voltage shield (SQ-239) and the cable assembly, lamp power supply (SQ-317),
from the lamp assembly.
63
4.
Disconnect the power supply cables from the +OUTPUT and -HV connectors in the ILC power
supply module.
5.
Replace the cable set and reverse numbers 1 through 4 to reassemble the unit.
6.
Ignite the CW lamp and run a Signal-to-Noise ratio test to ensure that the instrument is operating
normally.
Step 5: Check for faulty electrical connections
CAUTION:
THESE CHECKS SHOULD BE PERFORMED WITH POWER REMOVED FROM
THE INSTRUMENT.
1.
Check that the CW lamp power supply cables are secured to the side of the lamp assembly.
2.
Check that the CW lamp power supply cables are making good connection on the +OUTPUT and
-HV connectors of the ILC power supply module. It may be necessary to compress the lug
connectors on the CW lamp power supply cable and ILC power supply -HV connector with a pair of
needle nose pliers.
3.
Check the solder joints on the power supply end of the CW lamp cables.
4.
Check for corrosion on all connectors. Clean the connectors if necessary.
Step 6: Perform CW lamp housing assembly upgrade
These changes have been made on all recent models. There may, however, be a few of the older
instruments that still have the old style lamp house.
The changes to the lamp house include the following:
(Refer to Figure 52 Light Tube Assembly – Sheet 2)
a. Four fins of the front heat sink cathode (SQ-278) are cut back at an approximate
45 degree angle. This was done to increase the distance between the top fins and the
top faceplate mounting screw.
(Refer to Figure 51 Light Tube Assembly - Sheet 1 )
b. Two dowel pins have been removed from between the faceplate (SQ-196) and the CW lamp
mount (SQ-194).
c.
(Refer to Figure 51 Light Tube Assembly - Sheet 1)
Teflon standoff spacers (M-4025) have replaced three metal standoffs. Three of these spacers
are required to upgrade the lamp house assembly.
Perform this upgrade if you encounter a lamp housing that has not been modified as described above.
1. Remove power from the system.
2. Remove the lamp housing from the baseplate.
3. Remove the light baffle (SQ-251) by removing the two screws (H-0641).
4. Remove the front faceplate (SQ-196) by removing the three Phillips screws (H-0730).
64
5. Remove two dowel pins from the faceplate.
6. Clip the four fins from the front heat sink at a 45-degree angle as shown in the drawing.
7. Replace the three standoff spacers with Teflon spacers (PN: M-4025).
8.
Reassemble the lamp housing and test for proper operation.
Procedure 14. PMT cooling upgrade
The cooling fan added in this procedure uses ambient air to control the thermal dissipation of heat that is
generated by the CW lamp. This upgrade procedure will improve the system sensitivity and stability by
cooling the emission photomultiplier tube. The heat created by the CW lamp heated the PMT, reducing its
sensitivity.
Some systems have both a continuous wave (CW) and Flash Lamp installed on the unit. The systems
that use both lamps will require some additional steps to complete the upgrade (italicized text).
Included in this procedure are the necessary steps to verify that the CW lamp simmer current is set to the
maximum new default setting. Once completed, this procedure will improve CW lamp starting and
increase lamp life.
Upgrade Kit Components:
1. An instrument cover with vent holes at the front left side and a “Flash Lamp warning label” on the top
of the cover behind the sample chamber cutout.
2.
A front power supply cover with biscuit fan mounted on top.
3.
A PMT housing with cooling fins.
4.
A fan house.
5.
An air baffle.
6.
A PCB circuit board assembly, SQ-322.
7.
A zip-lock bag containing:
(1) 4-40x7/8
SOS (stand off screw)
(8) 4-40x1/4
SST PHL SCR. (standard Philips screw)
(1) 4-40x1-1/4
SST PHL SCR. (standard Philips screw)
(4) 6-32x3/8
BLK SOC SCR. (black socket head screw)
(1) black cable clamp
(1) splitter cable
(1) AC tap cable
65
REQUIRED TOOLS:
•
Philips head screwdrivers (large and medium).
•
Flat blade screwdriver (large).
•
Hex socket drivers (1/16, 7/64 and 9/64).
•
Hex wrench (3/8).
•
L-shaped calibration pin (1/8" D x 2").
•
A black cloth (about 3' x 3').
•
Clean de-ionized water in a clean quarts cuvette.
Preinstallation System Checkout:
NOTE: In this procedure, you will monitor and record the premodified condition of the
instrument. This will be used as a reference point to verify the sensitivity of the
instrument after the upgrade is completed.
1.
Enable CW lamp and turn instrument ON.
NOTE:
Let the lamp warm up for ten (10) minutes.
2.
Start the AB2 software.
3.
Check monochromator slit calibration:
- Set the monochromators to 16nm.
- Ensure that both slits calibrated correctly by inserting the alignment pin into the
monochromator. It should slide in freely.
4.
CW lamp / reference channel baseline:
- In the Instrument Setup, select “Channels.”
- Add “Reference” to the “Channels” list.
- Set the Excitation Wavelength to 350nm.
- Set the Excitation Slit to 4nm.
- Write down the numerical Reference channel value displayed.
5.
PMT baseline:
- In the Instrument Setup, select “Channels.”
- Add “Emission left” to the “Channels” list.
- Open Emission shutter.
- Place a cuvette of distilled water in sample holder.
- Set emission parameters.
a) Set the emission monochromator wavelength to 397nm and the slit to 4nm.
- Autorange HV to get about 6V of PMT (EM) signal.
- Record the Emission (EM) signal value and PMT HV level.
6.
Flash Lamp baseline (if Flash Lamp is present):
- Turn CW lamp off by flipping “LAMP ENABLE” switch to OFF.
- Enter Instrument Setup; select “Light Source.”
- Select “Flash” as light source. Select “Continuous” and “Fluorescence” and let the lamp
warm up for one minute.
- Click on box to disable lamp to run only during acquisition
- Repeat steps 4 and 5 to get the Ref, PMT and HV values for the Flash Lamp.
- Record the Emission (EM) value and PMT HV level.
7.
Exit AB2 software and turn off the instrument.
66
NOTE: Do not turn off the computer unless you go through the shut down
procedure. Doing so can result in possible computer crash and will require
that you re-install the operating system.
System Upgrade Procedure:
1.
Turn the instrument off and unplug the AC cord.
2.
Disassemble the instrument:
- Remove the instrument cover.
- Remove the PMT shield (immediately re-install the screw to secure the instrument leg).
- Unplug the cables from the top of the power supply bucket and remove the power supply
front cover by removing the cover screws.
- Remove the CW lamp housing and set it next to the instrument.
CAUTION:
-
3.
If a Flash Lamp is present, unplug the Flash Lamp from the FL power supply and unplug
the FL power supply cable. Remove the FL power supply from the top of the power
supply by removing retaining screws located at the base of the FL power supply and set
unit aside.
Remove the PMT, housing and power supply:
CAUTION:
4.
BE SURE TO HANDLE THE LAMP ASSEMBLY WITH CARE.
DO NOT EXPOSE THE PMT TO ANY STRONG LIGHTS, SUCH AS
OVERHEAD FLUORESCENT LIGHTS OR SUN LIGHT. USE A BLACK
CLOTH TO PROTECT PMT FROM ANY POSSIBLE EXPOSURE AND
KEEP THE ROOM AS DARK AS POSSIBLE. ALSO, DO NOT TOUCH THE
PMT BULB WITH YOUR BARE HANDS. IF YOU DO TOUCH THE PMT
WINDOW, THE PMT ENVELOPE SHOULD BE CLEANED WITH ALCOHOL
BEFORE IT IS PUT AWAY.
Remove PMT mounting screw.
Cover area around the PMT with a black cloth, remove PMT socket from housing. Do
not remove the PMT from the socket.
Wrap the PMT and socket with a black cloth and store it in a dark and safe place, such
as inside a drawer.
Remove the PMT housing from the emission monochromator.
Remove the power supply from the unit by removing the screws along the outer edge of
the power supply.
Install SQ-322 PCB assembly in power supply bucket:
- Remove connector mount and isolation transformer (the one with screw-on terminals)
from power supply bucket.
- Replace the old PCB in the bucket with the SQ-322 using 4-40x1/4 SST PHL screws.
Discard the thumbscrews.
- Connect all cables the same as before in the power supply.
- Plug the “AC tap cable” to P1 of the SQ-322.
- Install the isolation transformer. Connect the open wires of the “AC tap cable” to the
left-most “0" and “115" terminals of the five terminal block, matching the color to the
wires already on the terminals.
- Install the connector mount. Plug the hour meter into the PCB.
67
5.
Increase the CW lamp simmer current to full power:
- On the ILC CW lamp power supply module, turn R15 (MOD MIN) fully clockwise, which
sets the simmer current to full power.
6.
Reassemble the instrument:
- Place power supply back into the unit and secure with the retaining screws that were
removed earlier.
- Install new PMT housing (with cooling fins) to the emission monochromator using
6-32x3/8 BLK SOC screws. Transfer the BLK SOC screw (used for locking down the
PMT socket) to the new housing.
- Install PMT and socket to housing. Make sure that the PMT is seated flat on the socket.
When inserting the PMT into housing, be careful to cover the general area using the
black cloth to minimize light leak and not to bump the PMT. Make sure the “lock down”
screw is extended into the groove of the socket. Plug the HV, PMT and Mask cables into
respective connectors as before.
- Install new front power supply cover (biscuit fan already mounted).
- If Flash Lamp is present, ground the Flash Lamp cable ring terminal (green wire) by
using one of the mounting screws.
- Install the power supply back cover.
- If Flash Lamp is present, connect Flash Lamp cable and FL power supply cable as
before.
- Install CW lamp. Tighten the long posts using a wrench.
CAUTION:
-
Be careful not to overtighten the post. They will easily become stripped
if excessive force is used.
If Flash Lamp is present, run FL power supply cable around and away from CW lamp
cables. Push the 4-pin Burndy connector (through the hole) underneath the base plate.
Make sure that the PMT is seated all the way in the socket. Lubricate the O ring with
petroleum jelly.
Install the standoff on the PMT housing and fan house using one each 4-40x1/4and
4-40x1-1/4 screws. Feed fan cable through notch; do not pinch cable. Use the black
cable clamp to route cables (from PMT socket) around the fan house.
Install air baffle perpendicular to the fan housing.
Plug the “Splitter cable” into the “D/C FAN” connector. Plug the lamp fan and biscuit fan
cables into the “Splitter cable.” Plug the cables to “D/C OUT” and “PMT MONITOR”
connectors as before.
System checkout and PMT adjustment
1.
Checkout:
- Flip “LAMP ENABLE” switch OFF. Plug in the AC cord and turn on the instrument. Verify
that the lamp fan and biscuit fan are operating. Also, verify that the card cage is powered
up and that the cage fan is operating.
- Verify that the Green LED for the CPU appears.
- Turn the instrument power OFF. Flip the “LAMP ENABLE” switch to ENABLE, and then
turn the power ON.
- Verify the following sequence of events.
a) The CW lamp starts and stays in high beam, and CW lamp fan is working
b) If CW lamp refuses to start, the ignition pulsing stops after about 2.5 seconds.
c) The card cage powers up about 3.5 seconds after the instrument is turned on.
- Verify that the “LAMP MONITOR” LED on the front of the instrument is on.
- Verify that the hour meter “clicks” and “advances” every few seconds.
68
2.
Adjust PMT:
- Let the CW lamp warm up for ten (10) minutes.
- Start the AB2 software. Check calibration of both slits as Preinstallation System
Checkout section, step 3.
- Check Ref value as in Preinstallation System Checkout section, step 4. The final Ref
value should be at least 90% of the preinstallation value. Refer to the value on
worksheet under post-installation portion of the procedure.
- Enter the emission wavelength and slit parameters, and HV value as recorded in
Baseline section, step 5.
- With the “lock down” screw loose but still in the groove, rotate the PMT socket to get the
highest PMT value, then lock down the PMT socket. The final PMT value should be at
least 80% of the preinstallation value.
- Refer to the value on worksheet under post-installation section for PMT levels.
- Install the air baffle suing two 4-40x1/4 SST PHL screws.
- Flip “LAMP ENABLE” switch to OFF. Verify that the CW lamp goes off. Flip the switch a
few more times and verify that the lamp does not try to re-ignite.
3.
Check Flash Lamp if present:
- Repeat Preinstallation System Checkout section, step 6, and verify that:
a) The final Flash Lamp Ref value is at least 90% of the preinstallation value. Refer to
the value on the worksheet.
- Using the HV value as recorded in Baseline section, step 6, the final Flash Lamp PMT value is at
least 80% of the preinstallation value. Refer to the value on the worksheet.
4.
Put the new cover (with vent holes in the front) in place.
5.
Verify that all the system, computer, plotter, and printer cables are re-connected.
6.
Test all system and computer functions to verify that all system components are operating
correctly.
Run a Raman line of distilled water with the same parameters used for baseline. Signal-to-Noise
should be 90011 or greater.
69
Parts List
DESCRIPTION
PART NUMBER
Power Supply Module
SQ-302
Continuous Wave Xenon Lamp
complete assembly with lamp, heat sinks,
fan and exhaust tube.
SQ-310
Continuous Wave Xenon Lamp individual components:
1.
Ozone gasket
2.
Ozone ceramic insulator
3.
150W xenon lamp
4.
Anode heat sink
5.
Cathode heat sink
6.
Brass clip
7.
Heat sink ring
8.
Contact fingers
SQ-258
SQ-170
S-0059
SQ-279
SQ-278
E-0685
E-1223
E-1202
Flash Lamp /w Power Supply Module
SQ-311
Lamp Change Mirror
(used when Flash Lamp is installed)
SQ-312
Flash Lamp Only
S-0057
Flash Lamp Power Supply Module
E-1286
Photomultiplier Tube (PMT)
SQ-275
Electronics Card Cage
* Not a field replaceable item
SQ-303
CPU/Analog Board
SQ-304
HV/MONO Control Board
SQ-305
Filter Wheel/Polarizer Board
SQ-306
Lamp On/Power Delay Board
SQ-302
Excitation Monochromator
SQ-301
Excitation Polarization/Filter Wheel
SQ-320
Emission Monochromator
SQ-301
Emission Polarization/Filter Wheel
SQ-320
GPIB Board (16-bit bus interface)
AT-style computers 3.5” media
S-0136
GPIB Board (16-bit bus interface)
AT-style computers 5.25” media
S-0137
70
GPIB Board (Microchannel interface)
PS/2 models only
S-0138
GPIB Interface Cable
S-0134
Fuse, 2.5 amp
(220/240 volt systems only)
A-1665
Fuse, 1.5 amp
E-0686
Fuse, 3/8 amp
E-0403
Fuse, 5 amp
E-0406
External PMT accessory
SQ-318
71
Figure 36 Monochromator Wiring
72
Figure 37 PCB Card Cage Sheet 1
73
5/8
E-2015
WIRE
18 AWG GRN
10" LG
EC-133
RG-58 BNC CABLE ASSY
(CUT ONE EC-133 TO
LENGTHES SHOWN)
H-0204
RING # 6
TERMINAL
(CRIMP & SOLDER)
5/8
CUT AWAY 1/2" OF
CONDUCTORS 25 & 26
(LAST 2) THIS END.
E-2015
WIRE
18 AWG GRN
6" LG
(SOLDER TO BRAID)
H-0204
RING # 6
TERMINAL
(CRIMP & SOLDER)
H-0241
TUBING, SHRINK
3/8" BLK
E-0192
CON-RECP
26-PIN
E-0383
WIRE
18 AWG YEL
2" LG
E-0267
CON-RECP
UHF
330000-59 7
CABL E
26 CND
20" LG
PIN 1 ON
RED TRAC ER GOES TO
PIN #1 ON BOTH ENDS
E-1347
CON-RECP
24-PIN
RED TRAC E
INSIDE
PIN 1 ON OUTSIDE
3/4
E-0393
CABL E
22GA
35" LG
H-0223
TUBING, SHRINK
3/16" BLK
A-4160 (3)
TUBING, SHRINK
1/16" CLR
SHRINK ON ALL (3) CONNEC TIONS
NOTE: MATCH WIRE COLOR
TO SHAPE OF LEADS
H-0232 (2)
TUBING, SHRINK
1/8" BLK
A-0753
TUBING, SHRINK
3/16" CLR
2" LG
E-0127
CON-ACCY
STRAIN RELIEF
BLK
RED
GRN
(DO NOT SHRINK)
E-1191
CON-ACCY
STRAIN RELIEF
E-0318
LED LAMP
E-0235
CON-RECP
6-PIN
FL AT
ORG
WHT
E-1188
CON-RECP
2-PIN
E-0384
FAN DC
RED BLK
RED BLUE
E-2083
CABL E 22GA
CUT TO 1.5"
CUT TO 1.5"
YEL
E-0667
SWITCH
SPDT
H-0223 (3)
TUBING, SHRINK
3/16" BLK
ALL WIRES 24 AWG
8" LONG
A-0753
TUBING, SHRINK
3/16" CLR
6" LG
1 - BLK
2 - RED
3 - GRN
(DO NOT SHRINK)
PIN #1 - RED
PIN #2 - BLK
SQ-326-S2, Rev. F
TAKE INSIDE WIRES OUT
OF CABLE BEFORE USE
Figure 38 PCB Card Cage Sheet 2
74
4 - YEL
5 - ORG
6 - WHT
Figure 39 PCB Card Cage Sheet 3
75
Figure 40 PCB Card Cage Sheet 4
76
Figure 41 CPU/Analog Bd. Schematic – Digital Section
77
Figure 42 CPU/Analog Bd. - Analog Section
78
Figure 43 CPU/Analog Bd. - Flash Lamp Control
79
Figure 44 CPU/Analog Bd. - GPIB Section
80
Figure 45 CPU/Analog Bd. Assembly Drawing 1 of 2
81
Figure 46 CPU/Analog Bd. Assembly Sheet 2 of 2
82
Figure 47 Mono/Slit/HV/Shutter Sheet 1
83
Figure 48 Mono/Slit/HV/Shutter Sheet 2
84
Figure 49 Filter Wheel Control
85
Figure 50 Lamp On & Power Delay Board
86
Figure 51 Light Tube Assembly - Sheet 1
87
Figure 52 Light Tube Assembly – Sheet 2
88
Figure 53 Electronic Bucket Assembly
89
90
Thermo Spectronic
A Thermo Electron business
5225 Verona Road
Madison, WI 53711-4495
Tel: (800) 201-8132 or (608) 276-6100
Fax: (608) 273-5046
Email: [email protected]
Web: www.thermo.com/spectronic
INSTALLATION REPORT
Customer Name
Rep Name
Institution
Rep Organization
Department
Address
EX Calibration Value
EM Calibration Value
Signal/Noise Value
CW Lamp:
Phone
Reference Channel Value
Purchase Order #
EM Signal Value
Installation Date
PMT HV Value
Instrument Type
Flash Lamp:
Serial Number
Reference Channel Value
EM Signal Value
PMT HV Value
Accessories Included
Comments
Service Engineer
Customer
Thermo Spectronic
A Thermo Electron business
5225 Verona Road
Madison, WI 53711-4495, USA
Telephone: (800) 201-8132 or (608) 276-6100
Facsimile: (608) 273-5046
Email: [email protected]
Web: www.thermo.com/spectronic
AMINCO-Bowman is a registered trademark of Thermo Spectronic
Thermo Spectronic is an ISO 9001 Systems Certified Company.
AB2-10020, Rev. A, 02/03
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