pco.edge family pco.

pco.edge family pco.

user manual

pco.edge family

pco.

Target Audience: This camera is designed for use by technicians, engineers and scientists.

In case of any questions or comments, please contact us at PCO.

telephone +49 (0) 9441 2005 50 fax email

+49 (0) 9441 2005 20 [email protected] postal address PCO AG

Donaupark 11

93309 Kelheim, Germany

The cover photo shows an exemplary PCO camera system.

The lens is sold separately.

Copyright © 2015 PCO AG (called PCO in the following text), Kelheim,

Germany. All rights reserved. PCO assumes no responsibility for errors or omissions in these materials. These materials are provided as is without warranty of any kind, either expressed or implied, including but not limited to, the implied warranties of merchantability, fitness for a particular purpose, or non-infringement. PCO further does not warrant the accuracy or completeness of the information, text, graphics, links or other items contained within these materials. PCO shall not be liable for any special, indirect, incidental, or consequential damages, including without limitation, lost revenues or lost profits, which may result from the use of these materials.

The information is subject to change without notice and does not represent a commitment on the part of PCO in the future. PCO hereby authorizes you to copy documents for non – commercial use within your organization only. In consideration of this authorization, you agree that any copy of these documents, which you make, shall retain all copyright and other proprietary notices contained herein. Each individual document published by PCO may contain other proprietary notices and copyright information relating to that individual document. Nothing contained herein shall be construed as conferring by implication or otherwise any license or right under any patent or trademark of PCO or any third party. Except as expressly provided, above nothing contained herein shall be construed as conferring any license or right under any PCO copyright. Note that any product, process, or technology in this document may be the subject of other intellectual property rights reserved by PCO, and may not be licensed hereunder.

Released: December 2015 © PCO AG pco.edge family User Manual V2.20 © PCO AG, Germany

TOP

TABLE OF CONTENTS

1. INTRODUCTION

1.1 DATA OVERVIEW

2. SAFETY INSTRUCTIONS

3. SYSTEM COMPONENTS

4. INSTALLATION

4.1 CAMERA DRIVER

4.2 CAMWARE

5. QUICK START

5.1 PREPARATION

5.2 START

5.3 YOUR FIRST IMAGE

6. CAMWARE 4 SOFTWARE

6.1 INTRODUCTION / CONTENT

6.2 CAMERA OVERVIEW / LIST

6.3 CAMERA PROPERTIES

6.3.1 TIMING

6.3.2 ROLLING SHUTTER

6.3.3 GLOBAL SHUTTER

6.3.4 GLOBAL RESET

6.3.5 IMAGE SIZE

6.3.6 SENSOR CONTROL

6.3.7 RECORDING CONTROL

6.3.8 I/O SIGNALS TAB

6.3.9 CONVERT CONTROL

6.4 RECORDER TOOLS

6.5 VIEW WINDOW

6.6 RECORDER (IMAGES)

6.7 CAMWARE TABS & FEATURES

6.7.1 DEMO MODE

6.7.2 FILE MENU

6.7.3 CAMERA MENU

6.7.4 ACQUISITION MENU

6.7.5 VIEW MENU

6.7.6 WINDOW MENU

6.7.7 HELP MENU

6.7.8 RIGHT-CLICK MENU

6.7.9 ADDITIONAL FEATURES

3

5

6

7

8

11

11

11

12

9

9

10

37

38

39

40

30

31

33

35

43

44

44

45

40

41

42

45

47

19

23

26

28

13

13

14

15

16

APPENDIX

A1 TECHNICAL SPECIFICATIONS

A2 CHANGE FROM F-MOUNT TO C-MOUNT

A3 WATER COOLING OPTION PCO.AQUAMATIC II

A3.1 SYSTEM COMPONENTS

A3.2 FIRST TIME INSTALLATION

A3.3 OPERATION

A3.4 DIMENSIONS

A4 INTERFACES

A4.1 CABLE POSSIBILITIES

A4.2 CAMERA LINK

A4.2.1 INSTALLATION OF FRAME GRABBER

A4.2.2 IMAGE DATA FLOW

A4.3 USB 3.0

A4.3.3 HARDWARE RECOMMENDATIONS

A4.3.4 USB 3.0 FAQ

A4.3.5 INSTALLATION OF THE USB 3.0 CARD

A4.3.6 RING BUFFER AND FIFO

A5 BINNING IN CMOS SENSORS

A6 LIGHTSHEET SCANNING MODE

A7 IMAGE FILE FORMATS

A8 CUSTOMER SERVICE

A8.1 SERVICE AND MAINTENANCE

A8.2 TROUBLE SHOOTING

A9 INDEX

ABOUT PCO

69

71

71

72

73

74

56

56

57

57

59

61

61

63

65

66

67

68

48

49

51

52

52

53

54

55

4

1 INTRODUCTION

1. INTRODUCTION

Advantages of the pco.edge family

The pco.edge family is a breakthrough in scientific imaging cameras. It has the distinctive ability to simultaneously deliver extremely low noise, high frame rates, wide dynamic range, high quantum efficiency, high resolution and a large field of view - all in one image.

The camera’s main features (model-specific)

ultra low noise down to 0.8 electrons med

high resolution up to 5.5 megapixel

best dynamic range up to 40000:1

high-speed up to 100 fps @ full resolution

high quantum efficiency up to 80%

flexibility user selectable choice of shutter mode

free of drift stabilized Peltier cooling in order to avoid any drift phenomena in image sequences

Areas of Application

• live cell microscopy

• single molecule detection

• localization microscopy

• lightsheet microscopy

• selective plane illumination microscopy

• SPIM

• structured illumination microscopy

• SIM

• TIRF microscopy / waveguides

• spinning disk confocal microscopy

• genome sequencing (2nd and 3rd gen)

• FRET

• FRAP

• lucky astronomy / imaging

• adaptive optics

• solar astronomy

• fluorescence spectroscopy

• bio- & chemiluminescence

• high content screening

• photovoltaic inspection

• x-ray tomography

• ophthalmology

• flow cytometry

• biochip reading

• machine vision

• spectral (hyperspectral) imaging

• laser induced breakdownspectroscopy (LIBS)

5

1.1 DATA OVERVIEW

Overview – Available camera models

This table shows an overview over all available camera models.

Type

Interface

Shutter

Read Out

Frequency

Line time

(µs)

FPS Sensor

Rolling 105 MHz 24.93 50

pco.edge

3.1

USB 3.0

48 mono & color

pco.edge

4.2 LT pco.edge

4.2 pco.edge

4.2

USB 3.0

USB 3.0

Camera

Link

Global 204 MHz

Global

Reset

105 MHz

Rolling 110 MHz

12.80

24.99

24.10

Global

Reset

110 MHz 24.10

Rolling 110 MHz

Global

Reset

110 MHz

Rolling

24.10

24.10

95.3 MHz

(slow scan) 27.60

272.3 MHz

(fast scan)

9.65

50

40

40

40

40

35

100 mono mono mono

pco.edge

5.5

USB 3.0

Rolling 86 MHz 30.5

Global 160 MHz

Global

Reset

86 MHz

Rolling

16.40

30.51

95.3 MHz

(slow scan) 27.52

286 MHz

(fast scan)

9.17

30

28

30

33

100 mono & color

pco.edge

5.5

Camera

Link

Global 286 MHz 9.17 50 mono & color

Global

Reset

95.3 MHz

(slow scan) 27.52

286 MHz

(fast scan)

9.17

33

100

6

2 SAFETY INSTRUCTIONS

2. SAFETY INSTRUCTIONS

Please read the safety instructions completely

Never operate the camera in humid or dusty environments or in places with high amounts of X-ray radiation. Humidity, dust or Xrays could damage the camera.

To avoid the risk of water condensation, protect the camera against extreme changes of ambient temperature. If condensation enters the camera, there is the risk of electric shock.

To prevent damage to the camera, the system must be kept stable and protected against strong jolts or vibrations . The socket at the bottom of the camera is to be used for mounting purposes only.

Electric shock warning – Never slide any items through slits or holes into the camera because of the risk of electric shock if the voltage parts inside are touched.

Each time the camera is used, check the power cable for any damage .

Never position the cable in a way that it could become a tripping hazard .

Do not force the lens onto the camera.

To protect the lens connector thread from damage, use minimal force when attaching a lens to the camera.

If any of the following conditions apply, immediately switch off the camera, separate it from the power line and contact our customer support:

If the power cable or the power plug seems to be worn or damaged.

If liquids have penetrated the device.

If, after thoroughly reviewing the instruction manual, the device is still not operating properly.

If the camera has been dropped or the casing is damaged.

7

3. SYSTEM COMPONENTS

The camera system includes the following parts.

Camera Head

F-mount optical connection (standard)

For standard F-mount / SLR lenses and adapters.

C-mount ring provided (see appendix A2)

For standard C-mount and microscopy connectors.

DC Power Jack

(connect to power supply)

Input/Output

4x SMA connectors

2x input - 2x output

Interface

(user selectable)

Dual Camera Link full or USB 3.0

LED

indicates camera status

Green continuous: camera is booting

Green blinking: camera is ready for operation

Yellow blinking: recording on

Red blinking: error

Serial Number Tag

(on the bottom of the camera)

Mounting Thread

1/4-20 UNC mounting thread

Camera Link Grabber Card / USB 3.0 PCI Interface Card

PCI Express x4 Card (Camera Link

full) or

PCI Express x1 Card (2 x USB 3.0 connections)

A PCI Card with 4 x USB 3.0 connections is also available, contact PCO for further details. A PCIe x4 slot is necessary for this card.

Power Supply

Your system will be equipped with either a 24 V or a 12 V power supply, depending on the model you selected. (connector: Lemo FGG.0B)

Cable

CameraLink Cable (2x3m) or USB-A/USB-B cable (5m)

Digital Camera Tools (USB storage device content)

• Camware: software for camera control & image acquisition

• Camera driver & tools

• Software development kit (SDK) & demo programs in C and C++

8

4 INSTALLATION

4. INSTALLATION

1

You will find all necessary files on the accompanying USB storage device. You may also download the newest versions of our software, camera driver and third party software drivers from the PCO website

(www.pco.de).

Minimum system requirements:

4.1 CAMERA DRIVER

• Clock speed > 2 GHz

• RAM > 4 GB

• Windows 7 or higher

• 1280 x 1024 pixel resolution display

• USB 3.0 or Camera Link

There are two different camera interfaces available:

Camera Link (Frame Grabber)

When operating the camera with

Camera Link Interface: Please run the appropriate grabber driver installation with default settings.

For

detailed installation instructions please see A4 (microEnable IV

grabber instruction).

USB 3.0

When USB 3.0 is used as a camera interface, it is recommended to use the enclosed PCI Interface card. For detailed installation

instructions or further hardware recommendations, see A4.3.

Install PCO USB 3.0 Driver

For pco.edge USB 3.0 you always need to install the latest

USB

Driver version. After these two screens the driver is completely installed.

2

1 2

9

3

4.2 CAMWARE

The Camware Windows application software enables you to control every camera parameter or setting. Images can be displayed on a monitor and may be downloaded and stored. The USB storage device contains the installation files for the software for latest

Windows operating systems in 32 & 64 bit.

After a successful installation, you will find the program file

Digital

Camera Toolbox in your program directory and a Camware32/64

button on your desktop. Other helpful tools are also installed in the same directory.

To uninstall the Camware program, please use the Software feature under Windows’ System Control.

Please follow the installation wizard

1 will be installed only to user folder

- Then choose install directory

-

3 Choose components: Select additional drivers for Camera Link

Interface (Silicon Software Dll mE IV)

- After the next two screens installation is complete

2

4

10

5 QUICK START

5. QUICK START

5.1 PREPARATION

In order to get familiar with your new camera and software it might be helpful, if you first aim at an object that is easy to focus and that can be seen at standard light conditions.

Computer is turned on

Installation is finished (see chapter 4)

An appropriate lens is attached (remove cap) or the camera is attached properly to the microscope, spectrograph or other scientific device

Camera is connected to the PC (USB 3.0 or Camera Link)

Camera is connected to the power supply and ready

(green LED blinks)

5.2 START

Start Camware and the graphical user interface will start up:

NOTE

Always install latest Camware version to be able to use full function of your pco camera.

11

5.3 YOUR FIRST IMAGE

2 3

Please follow the instructions:

1

Camware must be started.

1

• Start live preview. 3

• Right-click in the view window and apply Auto Range Peak. 4

4

6

5

If you need to change

exposure time (e.g. the image is still either too dark or too bright), please go to chapter

6.3.2

If you want to record and save images, please see chapter

6.3.7 and chapter 6.5

for detailed information.

NOTE

Live preview: Useful for fast and easy camera adjustment and focusing. This command disables the Camera Control window if it is opened (except exposure time setting).

12

6 CAMWARE 4 SOFTWARE

6. CAMWARE 4 SOFTWARE

6.1 INTRODUCTION / CONTENT

PCO’s Camware is an excellent for camera control, image acquisition and archiving of images in various file formats.

Camware works with any kind of PCO camera. Please see our Website for the latest version of this software.

Chapter 6.2: Camera Overview / List: shows all connected cameras and all set recording profiles

Chapter 6.3 Camera Properties: main dialog for all camera settings:

6.3.1 Timing

Timing settings / Trigger modes /

Synchronization

6.3.2 Rolling Shutter

Explanation / Timing

6.3.3 Global Shutter

Explanation / Timing

6.3.4 Global Reset

Explanation / Timing

6.3.5 Image Size

6.3.6 Sensor Control

6.3.7 Recording Control

6.3.8 I/O Signals

ROI / Sensor format / Binning

CDI / Double shutter / Temperature

Recorder aquire mode / Timestamp /

Sequence trigger

Input - Output

6.3.9 Convert Control Dialog

Contrast, Saturation, Gamma…

Chapter 6.4/ 6.5 /6.6 describe the recording functions

Chapter 6.7 describes the available tabs (File, Camera Acquisation,

View, Window, Help) the right-click menu and additional features.

No camera connected

6.7.1 Demo Mode

6.7.2 File

6.7.3 Camera

Open, save and print files, Options…

6.7.4 Acquisation

6.7.5 View

6.7.6 Window

6.7.7 Help

Camera control; Settings; Rescan,

Setup…

Live preview; Acquire secquence;

Rec. memory settings; Rec. settings

B/W or Color window; Convert

Control, Image Browser

Cascade; Tile; Arrange; Close all

Logfiles, About

6.7.8 Right-click menu

6.7.9 Additional features

Zoom; Flip; Mirror; Rotate…

White Balance, Contrast, ROI by

Mouse, Short Cut list

13

2

6.2 CAMERA OVERVIEW / LIST

3

6

1

If closed, the Camera Overview window can be opened by selecting the

View tab and Toolbars

1

and Docking Windows

→ Camera Overview.

4

5

The

Camera Overview window allows you to manage several connected cameras. It displays a list of all connected PCO cameras. Camware is able to

2 close a connected camera. It is possible to define many different

settings for each camera (max. 30 sets per camera → add new to other cameras.

5

4

Live Preview can be started. When unfolded Preview shows a

small preview window 6 (always monochrome) integrated to the camera list.

6

Live preview gives the ability to adjust aperture and focus and to have a first look at your object.

Click and drag camera setting: If you want to copy e.g. Camera

Setting 1 to Camera Setting 4 just drag & drop Setting 1 to Setting

4 and Camware will ask you if you want to copy the settings. It is possible to copy each setting to every camera.

Important Setting (for cameras without internal memory)

Memory Allocation Dialog

If you want to change the number of recorded images in

Camware, you have to open the

Acquisition Tab (see 6.7.4)

and choose

Recorder Memory Settings.

This sets the number of images recorded in one sequence.

The maximum is defined by approved RAM size.

14

6 CAMWARE 4 SOFTWARE

1

6.3 CAMERA PROPERTIES

The Camera Properties window in Camware is the main interface for all camera settings. The active set selected within Camera List can be adjusted here.

The former main instance Camera Control (known from Camware

3.x) and the

Convert Control (see 6.3.9) can be opened additionally.

Three view options with various functions can be selected:

Standard,

Custom and Expert.

2

3

4

1 number and the exposure time. It is recommended for new Camware users.

2 be configured individually by the

Custom Properties Button.

4

Additional to the standard mode Trigger mode, Image Size and

Recording control options are selectable.

3 property settings.

An explanation for every setting is displayed below the properties dialog.

15

6.3.1 TIMING

Introduction to timing

An important parameter for a camera is the frame rate. The upper limit of the frame rate is defined by exposure and readout time.

The figure below shows the timing scheme. Exposure and readout are done simultaneously, this means while image

n is readout from the sensor, image n+1 is already integrated within the sensor’s pixel elements.

1 readout is the limiting factor. The second figure shows that for long exposure times the exposure time plus delay

(t delay

) is the limiting factor. readout exposure

t

exp

t

readout

n + 1

n + 1

n + 2

n + 2 readout exposure

n - 1

t

exp

t del

n + 1

n + 1

If a lower frame rate is desired, this can be achieved by inserting additional delay times. These rules also apply in external trigger mode, i . e . this defines when the next trigger can be applied or recognized.

For further timing explanations (e.g. fps based timing) please read the following chapters for Rolling Shutter, Global Shutter and Global

Reset.

16

6 CAMWARE 4 SOFTWARE

1

NOTE

If the trigger rate of the external signal is quite near the maximum possible frame rate

(difference < 1/1000), it will be random, whether or not a trigger is accepted!

Trigger Mode

1

In this context trigger means exposure trigger, i.e. the trigger signal controls the exposure of a single image (light integration time).

Auto Sequence: The camera will optimize the image recording to achieve the best possible frame rate.

In the auto sequence exposure control mode, the camera determines the fastest possible frame rate depending on the adjusted exposure time and the required readout time.

After a start command is given, the sequential recording is started until a stop command is given.

Soft Trigger: Single images can be recorded with this Camware command. The single image can be acquired by pressing the

Single Trigger button. This button appears after pressing the

Start Record button (see 6.4 ). Other signals cannot influence

this operating mode.

In the External Exposure Start exposure control mode, single image recording is started by the falling or rising edge of the voltage signal

at the BNC input #1(see

6.3.8). The frame rate cannot be set, as the

frame rate is defined by the frequency of the external signal. However the predefined exposure time and ROI settings affect the maximum possible frame rate.

The

Busy Status signal at BNC #3 (BNC explanation see 6.3.8) will

indicate if a new trigger is accepted.

Exp trig (in)

Exp Stat (out)

Busy Stat (out)

acc.

not acc. accepted accepted

The maximum achievable frame rate in external trigger mode is negligibly less (about 0.1%) than operating the camera in

Auto

Sequence mode.

If the trigger rate of the external signal is higher than the maximum possible frame rate, every second trigger pulse is ignored. Therefore the actual frame rate drops to half of the external trigger rate. If the trigger rate is increased further, then only every third, every fourth etc. trigger edge will be accepted.

17

Exp trig (in)

Exp Stat (out)

Busy Stat (out)

acc. not acc

. acc. not acc

. acc. not acc. acc. not acc.

In order to avoid trade-offs at maximum frame rate use either the

Busy Status signal or make sure that the external trigger rate follows this condition: 0.999 x External Trigger Rate

≤ f max

.

External Exp. Control An external signal applied at BNC #1 (see

6.3.8), controls the start and the duration of the exposure.

A new exposure is started by the falling or rising edge of the voltage signal at the BNC input. The exposure is finished when the opposite edge is detected. Thus in this mode, the start as well as the length of the exposure time can be controlled.

No further settings can be made, as the image timing is completely controlled by the external trigger signal.

Exp trig (in)

Exp Stat (out)

Busy Stat (out)

t readout acc. not acc. accepted accepted

Be aware, that the externally controlled exposure time is limited. The integration will be stopped automatically if the maximum exposure time is achieved.

The Busy Status signal at BNC #3 (see 6.3.8) indicates if a new

trigger is accepted.

Camera Interface pco.edge 3.1 pco.edge 4.2LT pco.edge 4.2

USB 3.0

USB 3.0

CameraLink

Shutter Mode

Rolling

Global

Global Reset

Rolling

Global Reset

Rolling

Max.exposure time

2 s

100 ms

2 s

10 s

2s

10 s pco.edge 4.2 pco.edge 5.5

USB 3.0

Camera

Link

USB 3.0

Rolling

Global

Rolling &

Global Reset

Rolling

20 s

100 ms

2 s

2 s pco.edge 5.5

18

6 CAMWARE 4 SOFTWARE

6.3.2 ROLLING SHUTTER

In Rolling Shutter mode the pixel reset and exposure start is carried out row by row. Each row has the same exposure time, but a different start and end of exposure. The pco.edge image sensor consists of two discrete halves, which are exposed and read out simultaneously, i.e. from the outside to the center by default. Within one row, the exposure starts simultaneously for all pixels. Available RS readout

modes see 6.7.3.

General Timing Diagram

reset start reset end readout start readout end t frame t global t frame t exposure (first row) t t t exposure (last row) t all rows

The exposure time of each row starts with the corresponding reset of the row. Then after a predefined time, the exposure is stopped. The light induced accumulated charge carriers of the pixels in a row are recorded into memory in a low noise (readout) mode. This results in the total image appearing in memory corresponding to the row readout.

Timing

The exposure and delay time can be adjusted in steps of one line

time (see p.

6).

Camera (RS only) Interface Exposure time Delay time pco.edge 3.1 USB 3.0 500 µs … 2 s pco.edge 4.2 LT USB 3.0 100 µs … 10 s pco.edge 4.2 pco.edge 4.2 pco.edge 5.5

Camera Link

USB 3.0

CL & USB 3.0

100 µs … 10 s

100 µs … 20 s

500 µs … 2 s

0 … 1 s

19

NOTE

Δ t = t : 1 line time

(See p

.6)

FPS based (only Camera Link interface)

The camera will optimize the image recording to achieve the selected frame rate with chosen exposure time as close as possible.

Only for

Auto Sequence trigger mode and only available with

Camera Link Interface

First the frame rate is set. If the time required for readout of the image is longer than 1 / frame rate, then the frame rate will be reduced to

1 / t readout

.

The frame rate can be adjusted in steps of 1 mHz (Rolling Shutter).

If the selected exposure time would require a lower frame rate, the exposure time is cut to the maximum possible time at that frame rate.

Camera

(RS only)

Interface Frame rate (FPS Based) Exposure time pco.edge 4.2

(@ full resol.)

Camera

Link

0.1…35 Hz @ 95.3 MHz

0.1…100 Hz @ 272.3 MHz

100 µs…10 s pco.edge 5.5

(@ full resol.)

Camera

Link

0.5…33.3 Hz @ 95.3 MHz

0.5…100 Hz @ 286 MHz

500 µs…2 s

Exposure time > Sensor frame readout time (Auto Sequence)

In case the required exposure time is longer than the frame readout time, the image sensor is completely exposed to light for some time

(t global

). In case of a triggered flash illumination, this would be the best moment to illuminate the image sensor. t exposure

1st row, 1st image t exposure

1st row, 2nd image

Trigger

(in)

Start

Busy

(out)

Start

Expos

(out)

Start

Global

(out)

Start t frame t all rows 1st t all rows 2nd

20

Δ t = t

6 CAMWARE 4 SOFTWARE

The hardware signal for the time t global

(Global out see

6.3.8).

is available on

connector #4

Obviously, if during exposure and readout, parts of the viewed image are moving horizontally, this would result in image distortion. This is why the global shutter mode may be a prerequisite for some applications.

However, most dynamic events can be captured in 1 ms, which is a common integration time with SLR cameras set at 1/1000 exposure.

The time shift from one row to another is only about 10 µs (fast scan).

The resulting maximum readout time of 10 ms (at full resolution) seems to be sufficient for a broad spectrum of dynamic events.

The 10 ms is also faster than the image shift process of most frame transfer emCCD image sensors previously used for low light applications. If this does not influence the image recording and processing, then rolling shutter mode will not affect it either.

Exposure time < Sensor frame readout time (Auto Sequence)

In case the required exposure time is shorter than the frame readout time, the image is composed of two exposure bands moving from the outside to the center of the sensor. exposure stop & readout band of simultaneous exposure reset & exposure start

For example, the shortest exposure time in RS is 500 µs (pco.edge

3.1 & 5.5) and 100 µs (pco.edge 4.2). The band of simultaneous exposure is in this case (smallest possible height) at full resolution:

e.g. pco.edge 4.2: 100 µs / 24.93 µs (line time) = 4 → number of

simultaneous rows = 8 (Rolling Shutter mode A-D see

6.7.3)

Previous comments on image distortion (also known as Rolling

Shutter Effect) apply here as well.

Graph on next page.

21

Auto Sequence graph

Trigger

(in)

Start

Busy

(out)

Start

Expos

(out)

Start t frame t all rows 1st image t all rows 2nd image

NOTE

The jitter t jit

can be a maximum of one row/line time.

Details for External Exp. Start and External Exp. Ctrl

The detailed timing for external trigger includes system delay times, an adjustable additional delay time, and the jitter.

Exp trig (in)

Exposure

Camera

(RS only) pco.edge 3.1 pco.edge 4.2

t rsys t delay

Interface

USB 3.0

Camera

Link

t jit

System clock

100

MHz

72 MHz

(t t sys rsys/ t fsys

)*

50 ns

70 ns

t fsys t delay

t jit

(jitter)

t jit

t delay

(delay) pco.edge 4.2 /

4.2 LT pco.edge 5.5

USB 3.0

USB 3.0

100

MHz

100

MHz

50 ns

50 ns

≤1 line time

(see p.

6)

0 … 1s pco.edge 5.5

Camera

Link

72

MHz

70ns

* t rsys/ t fsys

= t rising edge / t falling edge

For optimized synchronization (minimized jitter time) use the falling

edge of the line signal at the status output (see 6.3.2).

22

6 CAMWARE 4 SOFTWARE

6.3.3 GLOBAL SHUTTER

NOTE

Global Shutter is only available for pco.edge

3.1 and 5.5 Camera

Link & USB cameras.

First, all pixels are globally reset and these reset values are shifted into so-called diffusion nodes. From there, they are non-destructively read out into memory as reset dark images. The exposure starts after transfer of the reset dark image to the diffusion nodes, where they are stored on the chip. The exposure is stopped by global charge transfer to the diffusion nodes. Then, the exposure image is read out to the memory, where the former reset dark image is subtracted to perform an external correlated double sampling, which reduces the noise.

Since two images have to be read out to receive one resulting image, the sCMOS image sensor’s Global Shutter mode has only half of the frame rate of the

Rolling Shutter mode.

Reset Exposure Readout

Timing

The exposure and delay time can be adjusted in steps of one line

time (see page

6 ).

Camera (GS only) pco.edge 3.1 pco.edge 5.5 pco.edge 5.5

Interface

USB 3.0

Camera Link

USB 3.0

Exposure time

20 µs … 100 ms

10 µs … 100 ms

20 µs … 100 ms

Delay time

0 µs … 1 s

FPS based (only edge 5.5 Camera Link)

The camera will optimize the image recording to achieve the selected frame rate with chosen exposure time as close as possible.

Only for Auto Sequence trigger mode and only available with

Camera Link Interface.

First the frame rate is set. If the time required for readout of the image is longer than 1 / frame rate, then the frame rate will be reduced to

1 / t readout

. Minimum frame rate is 1 / max. exposure time.

The frame rate can be adjusted in steps of 1 MHz (Global Shutter).

If the selected exposure time would require a lower frame rate, the exposure time is cut to the maximum possible time at that frame rate.

Camera

(GS only) pco.edge 5.5

Interface Frame rate (FPS Based)

Camera

Link

10 … 50 Hz @ 286 MHz

Exposure time

10 µs … 100 ms

23

External Exposure Start

(Auto Sequence respectively)

Trigger (in)

Busy (out)

Exposure (out)

Frame t jit t t frame exp

# of lines line time

1

ROI (y) * line time

1

Programmable:

1 line time

1

… 100 ms t delay

(system) t if

1

line time → see p.

6

( t frame

– t exp line time

1

)

The listed parameters can be output via SDK function

Get Image

Timing dependent on the selected ROI.

NOTE

If t exp

< t frame system delay (t delay

) is added before exposure starts.

24

6 CAMWARE 4 SOFTWARE

External Exposure Control

Trigger (in)

Busy (out)

Exposure (out)

Frame t jit t frame t exp

1

line time → see p.

6

# of lines line time

1

ROI (y) * line time

1

Counted line time

1

In External Exposure Control trigger mode the external signal controls start of image acquisition and duration of the exposure.

First, all pixels are globally reset and these reset values are shifted into so-called diffusion nodes. From there, they are non-destructively read out into memory as reset dark images.

In this mode, the exposure starts always after the readout of the dark image is completed. The length of the exposure has been detected by the sensor from the trigger input. The exposure is stopped by global charge transfer to the diffusion nodes after the respective time.

Then, the exposure image is read out to the memory, where the former reset dark image is subtracted to perform an external correlated double sampling, which reduces the noise.

Since two images have to be read out to receive one resulting image and the exposure cannot start during readout time of the dark image, this specific

Global Shutter mode provides less than half of the frame rate of the

Rolling Shutter mode.

25

6.3.4 GLOBAL RESET

NOTE

Global Reset is available for pco.edge

5.5 CL and for all edge

USB 3.0 cameras.

All pixels are globally reset and the exposure starts for all rows at the same time. The exposure stop is carried out row by row; therefore the duration of the exposure is not the same for all pixels. The rolling readout improves the image quality, but due to the difference in exposure time, a flash illumination is recommended. The readout

(exposure stop) is done from the outside to the center.

General Timing Diagram

reset / exposure start readout start readout end t global t exposure (first row) t exposure (last row) t all rows t frame

The exposure time of all rows starts simultaneously. The exposure time of the first row stops after the predefined time, the following rows are read out from the outside to the center row by row. Please note that this leads to a different duration of exposure time for all rows.

Timing

The exposure and delay time can be adjusted in steps of one line

time (see p.

6).

Camera (GR) Interface Exposure time Delay time pco.edge 3.1 pco.edge 4.2 / pco.edge 4.2 LT pco.edge 5.5 /

USB 3.0 30 µs – 2 s

0 µs … 1 s pco.edge 5.5 Camera Link 10 µs – 2 s pco.edge 4.2 (

Camera Link Interface): Global Reset is not available.

26

6 CAMWARE 4 SOFTWARE

FPS based (only edge 5.5 Camera Link)

The camera will optimize the image recording to achieve the selected frame rate with chosen exposure time as close as possible.

Only for

Auto Sequence trigger mode and only available with

Camera Link Interface.

First the frame rate is set. If the time required for readout of the image is longer than 1 / frame rate, then the frame rate will be reduced to 1 / t readout

.

The frame rate can be adjusted in steps of 1 mHz (Global Reset).

If the selected exposure time would require a lower frame rate, the exposure time is cut to the maximum possible time at that frame rate.

Camera

(GR only) pco.edge 5.5

Interface

Camera

Link

Frame rate (FPS Based)

1…33.3 Hz @ 95.3 MHz

1…100 Hz @ 286 MHz

Exposure time

10 µs…2 s

Details for External Exp. Start and External Exp. Ctrl

The detailed timing for external trigger includes system delay times, an adjustable additional delay time and the jitter.

Exp trig (in)

Exposure

t rsys t delay t jit t fsys t delay t jit

Camera

(GR only) pco.edge 3.1 pco.edge 4.2 LT pco.edge 4.2

Interface

USB 3.0 pco.edge 5.5

Camera

Link

( t rsys t sys

/t fsys

50 ns

70 ns

) t jit

(jitter) t delay

(delay)

≤1 line time 1 0 µs …1 s pco.edge 5.5 USB 3.0 50ns

1

line time → see p.

6

For optimized synchronization (minimized jitter time) use the falling

edge of the line signal at the status output (see 6.3.8).

27

6.3.5 IMAGE SIZE

1

2

Region of Interest

1

3

The ROI (Region of Interest) selects only a part of the sensor to be read out.

Vertical ROI: In order to speed up the frame rate and to reduce the amount of image data, the selected ROI needs to be placed symmetrical to the horizontal center line.

Horizontal ROI: In order to reduce the amount of image data a horizontal ROI can be set. Please be aware, that changes in horizontal direction will not increase the frame rate. The decreased image size you see within Camware is a combination of reduced sensor resolution and software downsizing (Soft-

ROI).

NOTE

Software based ROI (Soft-ROI) is enabled within Camware by default (not available for USB 3.0 versions). Due to this functionality, the resolution of pco.edge cameras with

Camera Link interface can be adjusted in steps of 1 – 4 pixels. Since the readout architecture of these cameras is not able to address single pixels, this downsizing is done by software. If you work with Device Adapters (µManager,

Labview, etc.) or with our SDK, the Soft-ROI is disabled by default. For further information, please see the SDK description.

Camera Interface

ROI, horizontal increments

ROI, vertical increments

Min.

ROI

Vert. symm.

ROI pco.edge 3.1 USB 3.0 4 1 64x16 no pco.edge 4.2

Camera

Link

1 1 40x8 no pco.edge 4.2 /

4.2LT

USB 3.0 4 1 64x16 no pco.edge 5.5

Camera

Link

4 1 160x8 no pco.edge 5.5 USB 3.0 4 1 64x16 no

Controlling the Soft-ROI Option (only Camera Link version)

pco.edge 4.2 pco.edge 5.5

Camera

Link

Camera

Link

20

160

1

1

40x8

160x

16 yes yes

Under

HKey-Current-User/Software/PCO/

Camware create a DWORD value with the name DisableSoftROI. Set this value to 1 in order to disable Soft-ROI. Remove or set this value to

0 in order to enable Soft-ROI by default. Keep in mind that unsymmetrical ROI decreases FPS. Table below shows values for

Soft-ROI disabled.

28

6 CAMWARE 4 SOFTWARE

Binning

2

Binning combines neighboring pixels (in either the horizontal and vertical direction) to form super pixels. It increases the signal to noise ratio (SNR), reduces the readout noise of the resulting pixels and decreases the spatial resolution of the total image, which is recorded.

For further information see A5.

(pco.edge 5.5 CL: Binning only in Rolling Shutter mode)

Available Binning Modes:

H1xV1, H1xV2, H1xV4, H2xV1, H2xV2, H2xV4, H4xV1, H4xV2,

H4xV4.

Sensor Format

3

Camera pco.edge 3.1 pco.edge 4.2 pco.edge 4.2 / 4.2LT pco.edge 5.5 pco.edge 5.5

Interface

USB 3.0

Camera Link

USB 3.0

Camera Link

USB 3.0

Preselected Sensor Format

2048 x 1536

2048 x 2048

2048 x 2048

2560 x 2160

1920 x 1080

2560 x 2160

29

6.3.6 SENSOR CONTROL

3

1

2

Pixelclock

1

The pixel clock sets the clock frequency and therefore the image sensor readout speed.

(See overview table on page

6 for available readout

frequencies.)

B/W Noise Filter

2

In addition to the integrated static defect pixel list processing a dynamic noise filter can be activated here in order to remove so-called blinkers and high noise pixels. If you encounter unexpected aliasing effects, turn this filter off.

For the following settings please start

3 select Sensor (Misc.) Tab:

Conversion Factor

The conversion factor defines how many charge carriers

(electrons), which have been generated by light in the image sensor in each pixel, are necessary to generate one count (one intensity level) in the digital image. Therefore, the conversion factor describes the gain that is applied to the signal before it is converted into a digital value. The conversion is optimized for the pco.edge @

0.46 e /count.

Cooling Setpoint

Display of sensor temperature: A peltier cooling unit is used to keep the sensor's dark current to an acceptable minimum and in order to allow for a continuous operation free of any drift phenomena in image sequences. Either an internal fan or an external water cooling system assures proper heat transfer from the peltier element to regulate the temperature of the cameras.

Camera pco.edge 3.1 pco.edge 4.2 LT pco.edge 4.2/5.5 pco.edge 4.2 pco.edge 5.5

Interface

USB 3.0

USB 3.0

Camera Link

USB 3.0

USB 3.0

Sensor Temperature

5 °C

10 °C

5 °C

0 °C

5 °C

30

6.3.7 RECORDING CONTROL

1

3

2

Recorder Mode

1

Camware will use free RAM space on your computer. The recorded images will be temporally saved as 16bit multi TIFF. In

Sequence mode the recording stops when RAM space is full. In

Ring Buffer mode the camera will stop only by a stop command, hence overwriting previous images. For longer recording periods an appropriate RAID system is necessary, see also the

Direct Record to File option, see 6.7.2.

Acquire Mode

2

The acquire mode gives you the ability to enable or disable the recording by an external signal. If set to

Auto all images are accepted and all images taken are saved. A signal at the

acq

enbl input (see chapter 6.3.8) is ignored for this function.

Operation of the acquire mode depends on the

selected

trigger mode.

If set to External, the camera will only record images if the external signal enables recording.

Trigger mode Auto Sequence: This sensor timing scheme is paused by the signal at the acq enbl input. The acq enbl input is sampled at the beginning of the image generation, which can be seen at the rising edge of the busy stat output. If the

acq enbl input is high (low, when inverted) when an image is acquired, it causes an idle state until the acq enbl input is low (high, when inverted).

Busy Stat out)

Acq enbl (in) image image image image

In trigger mode External Exp. Start, the acq enbl input works like a gate for the trigger signal. A trigger edge (rising, falling when

exp trig is inverted) is accepted only when the

acq enbl signal is high (low, when inverted).

Acq enbl (in)

Exp trig (in)

Busy Stat

(out) accepted not acc. not acc. accepted accepted

In trigger mode External Exp. Ctrl, the acq enbl input works very similar to the mode External Exp. Start. However, the acq enbl input is ignored for the edge which is closing the exposure time (started exposure will be finished accordingly).

31

6 CAMWARE 4 SOFTWARE

When using acq enbl in external trigger modes, the following timing specification should be met:

Exp trig (in)

Acq enbl (in) t su t h

= 70ns

= 70ns

t su t h

If the acq enbl signal changes within the window of t su

(set up) to t

(hold), the behavior is random. The trigger may be accepted or ignored. h

Sequence Trigger

Once, a falling or rising edge at the

acquire enable trigger input

(see chapter 6.3.8) is recognized, an internal image counter

starts to run. It will count all acquired images and will stop the recording when the predefined number of images is reached.

Example timing diagram: Trigger mode = external exposure start;

Acquire mode = sequence trigger; Image counter = 3

Acq enbl (in)

Exp. trig. (in)

Status busy (out) not acc.

Image 1

Image 2 Image 3 not acc.

Timestamp

3

A time stamp can be placed into the upper left corner of the image. It can be either put off, binary or binary with text.

The time resolution is 1

μs. In binary mode the first 16 pixels will be filled with the time stamp information (binary code). The numbers are coded in BCD with one byte per pixel, which means that every pixel can hold 2 digits. If the pixels have more resolution than 8 bits, then the BCD digits are right bound adjusted and the upper bits are zero.

(1 BCD digit ≙ 4 bits; 2 numbers ≙ 2 BCD ≙ 8 bits = 1 byte; every pixel can hold 2 digits)

For further information please refer to the SDK.In binary and ASCII mode text will be placed into the image replacing the content of the image (271x 8 pixels). Time step shows the end of exposure time.

Three different information is stamped onto the image: number of

1 2 3

1

32

2

3

1

2

3

4

6.3.8 I/O SIGNALS TAB

Start Camera Control (see 6.3) and select the I/O Signals Tab.

1

2

3

4

1

Exposure Trigger

If checked, a signal for External Exp. Start or External Exp. Ctrl trigger

mode (see chapter 6.3.1) is accepted at the exp trig BNC input #1.

Aquire Enable

2

If checked, a signal for Acquire Mode or Sequence Trigger Mode (see

chapter

6.3.7) is accepted at the acq enbl BNC input #2.

Status Busy

3

If checked, a signal indicating busy status is given at the status busy output. Once an acceptable trigger edge is received,

busy will go on status

high. As soon as busy goes low again, a new trigger edge is accepted.

Status Expos

4

If checked, a signal indicating exposure status is given at the status output. Status Expos indicates the actual exposure window for one frame.

Status Line

4

If checked, a signal indicating line status is given at the status output.

Use the falling line edge for optimized synchronization (minimized

jitter time; see page 22).

Polarity

active for high/low signal or rising/falling edge

Maximum low level: 0.8V

Minimum high level: 2V

Slew Rate >1ms/V

Type

TTL Electrically grounded, no opt coupler.

3,3Volt LVTTL out , short time short-circuit-proof.

Continuous overvoltage withstand:

+10V and -5V.

1ms pulse overvoltage withstand:

+33V and -33V

ESD pulse: +- 4kV

33

6 CAMWARE 4 SOFTWARE

1

4

2

3

Rolling Shutter signal type

At the I/O-signal tab Rolling Shutter signal type options are available (only Status

Exposure and if Shutter is set to Rolling

Shutter, see

6.7.3 Setup.

There are four signal types available:

1

2 firstline

)

- Shows when all sensor lines are exposed (t

3

- Shows if any sensor line is integrating (t alllines global

)

) lastlinel

)

34

6 CAMWARE 4 SOFTWARE

6.3.9 CONVERT CONTROL

3

Start the Convert Control Dialog with the Black/White

Button in Camera Properties.

4

Convert Control BW

The user can influence how the 16 bit intensity values of the original image are displayed in 8 bit values in different ways.

1

2

BW Settings (includes histogram of original data)

It is possible to hide the histogram of original data and to switch tab/histogram .

Green sliders in histogram

3

2

left slider = Min controller (corresponds to value 0 of the 8 bit display). Values below that mark are set to 0, i.e. displayed as black.

right slider = Max controller (corresponds to value 255). Values above that mark are set to

255, i.e. displayed as white.

The values in-between are converted into a value between

0 and 255 according to Contrast and Gamma settings. See the small

graph , which reflects the calculation.

Proc config tab: please see under Convert Control Color

Converted Hist

This tab shows you the

histogram of converted data.

35

1

2

1

2

6

Convert Control Color (only pco.edge color)

3

7

4 5

Color Balance (Histogram of original data)

Intensity of single color can be controlled by

Saturation and Vibrance

.

Press the Auto button to set the

white balance

.

The balancing of RGB can be controlled by

3

Col.Temp and Tint

.

It is possible to

hide the histogram of original

4

5

The user can influence how the 16 bit intensity values of the original image are displayed in 8 bit values in different ways.

White sliders in histogram

6

left slider = Min controller (corresponds to value 0 of the 8 bit display). Values below that mark are set to 0, i.e. displayed as no color.

right slider = Max controller (corresponds to value 255). Values above that mark are set to 255, i.e. displayed as full color.

The values in-between are converted into a value between 0 and 255 according to

7 which reflects the calculation.

Converted RGB Hist

Proc. Config (Process configuration)

Due to proprietary high-end algorithms used for these image processing features, no detailed description is given here

3

4

5

1

This tab shows you the

histogram of converted data.

switch on in order to significantly reduce

processing time (increases refresh rate of

the live image

2

non local means algorithm

4

5

36

6 CAMWARE 4 SOFTWARE

6.4 RECORDER TOOLS

Play Speed: selectable play speed from x1 to x256 or from 1fps to 16fps. E.g. in mode x1 a recording with 1000 fps is played with 25fps.

1 fps means that only one frame per second is played.

Play Mode: selects play mode of the recorder

(continuous or single time (re)play).

Recording: in record state Camware software is highlighted in red.

Start/Stop record: with Record Button.

Software Trigger Mode: after record is started an arrow pointing downwards applies a single trigger.

Extended Recorder can be activated (see

6.7.5) via

Recording with multiple cameras:

If all cameras are activated the recording is started simultanously for all cameras.

Recorder will use Recorder mode settings (Sequence or Ring

Buffer) of the seleted camera for all cameras (see 6.3.7)

For single camera recording, deactivate cameras by removing the check mark from the box. 1

1

37

6.5 VIEW WINDOW

1

4

5

More View Windows

2

It is possible to open more view windows from one camera: just click on 1

2

Even when multiple view windows (or from multiple cameras) are open, the same image number is always shown in all of the view windows.

3 A

3 window. If you have more view windows than can be displayed on the desktop, you will be able to select each view window.

Split View Window

The view window can be split. Choose

Window

4 shown. You can easily adjust the size of the splitted window elements by grabbing and

5 dragging the dividing lines

5 .

The main function is that you are able to view four regions of your image in one view. Choose the Zoom± function to zoom in the image (first turn off

Stretched

View) (See 6.7.8 )

If you want to undo the split, you have to double click on the deviding line (after symbol

6

Two Tabs side by side or on top of each other

6

If you want to view two tabs side by side or arranged one above the other just drag a tab and then Camware will ask you if you want to create a new horizontal or vertical tab group.

Undo this very easily by draging the tab back to its former position.

This also applies for view windows of several cameras.

38

6 CAMWARE 4 SOFTWARE

6.6 RECORDER (IMAGES)

1

When recording is done, small preview images (thumbnails) are built and displayed automatically, this will take some time depending on the performance of your computer system.

1 adjust the number of images which are shown, by moving the mouse

(left or right).(minimum is 20 and maximum is half of the recorded

images in this scale)

Quick scrolling: you can quickly scroll through the thumbnails by dragging the orange bar with the mouse.

If you click on a thumbnail image it will be shown on the view window. You can scroll via mouse wheel through the thumbnails.

The upper

blue bar correlates to the number of displayed thumbnails. The lower blue bar shows the range of the upper scale in relation to the whole record.

3

2

The second scale shows the total number of recorded images. It allows to scroll fast through the

2

Right-click menu (click on thumb-nails):

Allows you to rebuild all thumbnails and to search for events.

Furthermore the Set In / Out gives you the possibility to set values for a sequence, which can be played via play button. Reset In / Out discards these settings.

If Set In / Out is active: if you save/export your images, only the selected images are saved/exported

(see

6.7.2).

The

3

In-Out example area. It is very easy to define a new area: just

right-click on the start and end frame in one of the scales. The In image must be left to the red bar, the Out image to the right of the red bar.

Search Events in Thumbnails: Detected events are displayed as

green bars

39

6.7 CAMWARE TABS & FEATURES

6.7.1 DEMO MODE

This chapter describes in detail the Camware Demo Mode and the

Camware Tabs: File, Camera, Acquisition, View and Window.

Furthermore the right-click menu and some additional features are listed.

When Camware is started, it automatically recognizes the camera type of the connected and running cameras.

Camware will start in Demo Mode, if your camera is switched off or no camera is connected

In this mode all

image processing features are available, but all camera settings are deactivated. The user only has to tell

Camware what type of image he wants to open. For that purpose, the

Demo Mode Setup window opens and asks for the corresponding input.

Need Help?

If this window pops up because you have trouble

running the camera, please see instructions in appendix

A8.

Resolution

The drop down list displays the existing image sensor spatial resolutions of all PCO camera systems. Please select the specific resolution and bit depth of the images to be opened. If double shutter images have been recorded and should be opened, Double Shutter

Mode should be checked.

Color

With the radio buttons, the user can specify whether the image type is monochrome (b/w) or color.

Alignment

These two radio buttons adjust whether MSB (most significant bit) aligned (upper) or LSB (least significant bit) aligned (lower) images have been stored.

Example settings to view the b16 files of the pco.edge:

Camera pco.edge

5.5 color pco.edge

4.2 mono

Resolution bit DoubleShutter Sensor Alignment

2560x2160 16 <no> color -

2048x2048 16 <no> mono -

40

6 CAMWARE 4 SOFTWARE

6.7.2 FILE MENU

Open RAW File (single image only)

This command should be used to import a single image into the currently active image window. Only files with the extension and format of

*.b16 (=PCO

proprietary binary image format) and *.tif (16 bit

TIFF image format) can be imported. If the recorder is enabled, each imported image will be transferred to the buffer shown in the picture number. The image itself will be fitted to the current image size.

If the recorder is disabled, the current image sizes will be set to the parameters of the imported image.

Open RAW Recorder Sequence (image sequence from one camera)

This command is used to import a sequence of images. If more than one camera is connected and an image window is currently open, the sequence will be loaded to the active window. If no image window is open, the images will be loaded to camera #1. This command opens the Open file dialog box. Only files with the extension and the format of *.

b16, *.pcoraw, *.tif and multi tif can be imported.

NOTE

Be aware of the different storage abilities of the formats, for example *.bmp - the bitmap format only allows for 8bit values to be stored and therefore the image content of a 16 bit image is reduced, if stored as bitmap.

Save RAW File (single image only)

This command should be used to save the image, which is displayed in the active window. The command opens the Save file dialog box.

The image file can be saved in 16bit *.b16 and *.tif format. If more than one camera is connected, it is possible to save all current images by selecting Export all images in the Save file dialog box.

With this feature it is possible to save one image of each active camera within one process step (it is not necessary to repeat the save process for each camera). The Save command will not be available, if no image window is open.

Save RAW Recorder Sequence (image sequence from one camera)

The Save Recorder command should be used to save or export image sequences. If more than one camera is connected and an image window is currently open, the record of the active window dialog box. It is possible to select the number of saved images, to step images and to choose the first image number.

will be saved. The command opens the Save recorder file

Export File (not reloadable!)

Use this command to export the image of the active image window.

This command will open the Export Image dialog box. Files with the extensions fts, tif, bmp, asc, jpg, and jp2 can be exported. This item will not be visible, if no image window is open

41

Export Recorder Sequence (not reloadable!)

Use this command to export a sequence of images.

If more than one camera is connected and an image window is currently open, the record of the window which has got the input focus will be saved. If no image window is open the Export

Recorder Sequence menu does not appear. This command will open the Export recorder box. Files with the extensions fts, tif, bmp, asc, avi, mpg, jpg,

jp2, and wmv can be exported (see Appendix A6).

Load Lookup Table (monochrome cameras only)

With the Pseudo LUT (Lookup-Table) feature you can load any LUT with one of four different formats and you can view the result in the color view window. Use one of the attached predefined LUTs or define your own.

Direct Record to File

This command is intended for cameras with internal memory. With this command you can preset a certain number of images to be stored. If the camera captures images faster than the computer can save to disk, then you will lose images. The displaying of the images doesn't interfere with the record process.

Exit

This command exits the program and closes all channel dialog windows. Window positions, settings and sizes are stored in the windows registry and will be loaded again at next start-up.

6.7.3 CAMERA MENU

Camera Control

Use this command to open the camera control window.

Close

Disconnects camera and switches Camware to Demo Mode. In case of multiple cameras, all cameras must be closed in order for

Camware to switch to Demo Mode.

Rescan

Disconnects and reconnects all cameras.

42

6 CAMWARE 4 SOFTWARE

A

C

E

B

D

A

C

B

D

6.7.4 ACQUISITION MENU

Setup

Switch between Rolling Shutter, Global Shutter and Global

Reset Mode. The camera will automatically restart.

Five different readout modes are available in

Rolling

Shutter (6.3.2). Standard mode is Dual Outside in. In

Single Top down, the pco.edge provides only half of the normal frame rate. Camware rotates the image that was recorded by the sensor, by 180°. This means, that the last line of the image is the first line of the sensor. PCO SDK names are from A to E.

E

Live Preview

The Live Preview is useful for fast and easy adjusting and focusing of the camera. The active window will be updated.

To see another window, simply click on the window. This option is not available in double shutter mode.

Acquire Picture (not available)

Acquire Sequence

Starts recording images into the system memory according to

Trigger Mode selection (see 6.3.1). During recording, all camera

controls are locked.

Rec. Memory Settings

This sets the number of images recorded in one sequence. The maximum is defined by approved RAM size.

43

6.7.5 VIEW MENU

6.7.6 WINDOW MENU

B/W or Color Window

Use this command to open a new display window.

Convert Control

See chapter

6.3.9.

Toolbars and Docking Windows

Standard Toolbars of Camware 4 are Recorder /

Recorder Tools / Camera View and Camera

Properties. Additional Toolbars know from

Camware 3.x are displayable, but not essentially needed: Main Toolbar / Extended Recorder /

Cursor

Application Look

The Style and Look of Camware can be customized; many different style sheets are selectable. The Tabbed

MDI function (un)docks the view windows.

Reset layout to default

This resets all your customized changes and restores the default layout.

New Window

A new view window will be opened.

Close Active Window

Active window will be closed.

Split

The view window will be split in four quarters.

Camera overview

Shows all connected cameras.

44

6 CAMWARE 4 SOFTWARE

6.7.7 HELP MENU

Contents

Opens the main page of the program help.

Search for Help on

Opens an index list for help.

Create Support File

This will activate the Camware log files. Press YES to activate log files and reboot Camware and your pco.edge.

After log files are activated it is possible to create a support file. Please send this file to the PCO support.

Logging

Enable Logging:

Activates Camware log files (this cuts down performance)

Clear Logfiles: (only visible if logging is enabled)

This command erases all actual log files

Explore Logfiles:

Opens windows explorer

Disable Logging (only visible if logging is enabled)

Disables logging

Support Mail

This command opens your email-program and an already created support file will be added automatically as file attachment.

About Camware

This window shows program information.

6.7.8 RIGHT-CLICK MENU

View Color: color window

View Window B: if Double Shutter mode is activated, this will switch to window B (second image)

Stretched View: image will be fitted into the display window

Stretched View Ratio: aspect ratio are maintained

Zoom +/-: image zoom (only available if Stretched View is deactivated)

45

Zoom set the factor of the Zoom (from 0.0625 to 32)

Scroll Synchronous if more than one image window is opened, it is possible to scroll synchronous through the images (only available if Stretched View is deactivated)

Show Image Info: resolution/ dynamic/ alignment / time.. are shown

Open LUT: opens look-up table file for false-color illustration

Auto Range Peak searches for the minimum and maximum 16 bit intensity values of the image. Given these numbers the converter scales the 8 bit display

(256) within these two values.

Auto Range Crop sets the converter to ignore the extreme intensity values of the image and scales the display in a smaller range. Thus dark or bright light spots, reflections, etc. are cut off.

Continuous Auto Range (Crop):

This option enables the automatic min/max function during record and replay.

Flip/Mirror: image will be flipped or mirrored

Set ‘File Save ROI’: if you want to save just a part of the recorded image (region of interest), it is possible to draw a rectangle with the mouse. This rectangle is valid for all recorded images and can be dragged at its edges.

Line Tool

Select

Line Tool to show a graph, which shows the intensity of the pixels under the red line shown in the image window. The selection of this menu item determines the location of the first point. After this you can move the mouse to the second point, which can be selected by a

left click. Now a Line Diagram opens and the length of the line (unit: pixel) is shown in a bubble and displayed in the window.

Copy to Clipboard: will copy the actual image to clipboard

46

6 CAMWARE 4 SOFTWARE

6.7.9 ADDITIONAL FEATURES

White Balance by Mouse

It is possible to change white balance easily by mouse: You only have to press the CTRL (Strg) and the shift button at the same time and select a white or gray area within the image. The pixel values within the coordinates of the selection rectangle are used for calculating a new white balance.

For best results we recommend to use the white balance button in

the Convert Control Color (see 6.3.9).

1

Fold Up Window

The Convert Control windows can be

1 over the bar and the window will unfold

2

2

Setting Contrast Area by Mouse

You can control the minimum and maximum values used for the conversion from 16 bit to 8 bit with the mouse. Move the mouse cursor into a region which should be shown with maximum contrast.

Press the shift and the left mouse button. Hold down the mouse button while increasing the size of the selection rectangle with mouse moves. After releasing the mouse button the coordinates of the selection rectangle act as a border for calculating the minimum and maximum values.

Setting a new ROI by Mouse

In the same manner you can setup a new region of interest (see ROI) for the camera. You only have to press the CTRL (Strg) button and drag an area with the left mouse button. The coordinates of the selection rectangle are used for calculating a new region of interest, which will be adapted to the camera capabilities automatically. You can reset the ROI to maximum by pressing the CTRL (Strg) button and the right mouse button.

Short Cut List

• Acquire Picture: SPACE

• Acquire Sequence: STRG + A

• Export File (Image): STRG + T

• Export Recorder Sequence (Video) STRG + O

• Open Raw Image File: STRG + I

• Open Raw Recorder (Video) Sequence: STRG + R

• Save Raw Image File STRG + E

• Save Raw Recorder Sequence (Video) STRG + S

47

APPENDIX

A1 TECHNICAL SPECIFICATIONS

A2 CHANGE FROM F-MOUNT TO C-MOUNT

A3 WATER COOLING OPTION PCO.AQUAMATIC II

A3.1 SYSTEM COMPONENTS

A3.2 FIRST TIME INSTALLATION

A3.3 OPERATION

A3.4 DIMENSIONS

A4 INTERFACES

A4.1 CABLE POSSIBILITIES

A4.2 CAMERA LINK

A4.2.1 INSTALLATION OF FRAME GRABBER

A4.2.2 IMAGE DATA FLOW

A4.3 USB 3.0

A4.3.3 HARDWARE RECOMMENDATIONS

A4.3.4 USB 3.0 FAQ

A4.3.5 INSTALLATION OF THE USB 3.0 CARD

A4.3.6 RING BUFFER AND FIFO

A5 BINNING IN CMOS SENSORS

A6 LIGHTSHEET SCANNING MODE

A7 IMAGE FILE FORMATS

A8 CUSTOMER SERVICE

A8.1 SERVICE AND MAINTENANCE

A8.2 TROUBLE SHOOTING

A9 INDEX

ABOUT PCO

71

71

72

73

74

49

51

57

59

61

61

56

56

57

63

65

66

52

52

53

54

55

67

68

69

48

A1 TECHNICAL SPECIFICATIONS

A1 TECHNICAL SPECIFICATIONS

Standard Version (pco.edge 3.1/ 4.2 (LT) / 5.5: Camera Link or USB 3.0)

Water Cooled Standard Version (pco.edge 4.2 / 5.5 Camera Link)

49

Water Cooled Standard Version (pco.edge 4.2 / 5.5, USB 3.0)

The housing size is similar to the dimensions of the standard version, except the camera design with the USB 3.0 interface allows to locate the water connections on the back of the camera.

Specifications:

General power supply power consumption weight operating temperature operating humidity range storage temperature range optical interface

CE / FCC certified

12 .. 24 VDC (+/- 10 %)

21 W max. (typ. 11 W @ 20 °C)

700 g (CL) / 930 g (USB)

+ 10 °C .. + 40 °C

10 % .. 80 % (non-condensing)

- 10 °C .. + 60 °C

F-mount & C-mount yes

Sensor type of sensor image sensor resolution (h x v) scientific CMOS (sCMOS)

CIS2020 CIS 2521

2048 x 2048 active pixel /

6.5 µm x 6.5 µm

2560 x 2160 active pixel

6.5 µm x 6.5 µm pixel size (h x v) sensor format / diagonal 13.3 mm x 13.3 mm /

18.8 mm fullwell capacity (typ.) 30 000 e- spectral range 370 nm .. 1100 nm

16.6 mm x 14.0 mm /

21.8 mm

30 000 e-

370 nm .. 1100 nm

50

A2 CHANGE FROM F-MOUNT TO C-MOUNT

A2 CHANGE FROM F-MOUNT TO C-MOUNT

Instructions on how to change the optical input from

F-mount to C-

mount:

Step 1: Remove F-mount Adapter

Grasp the F-mount adapter at the blue ring (counter ring) and turn it counter clockwise.

F-mount adapter

Step 2: Insert C-mount Ring

Carefully screw the ring completely in and tighten the two

Allen

screws.

C-mount ring

Adjust Back Focal Length

First of all, attach a lens to your c-mount adapter. Then set the focus of your lens to infinity. After that, look for a point in infinity* and generate a sharp image by turning the smaller ring of the adapter.

Then fix this position with the two small indented screws.

*rule of thumb: object should be away about 2000 times the focal length in mm

Allen screws

Limitations of C-mount lenses

Keep in mind that c-mount lenses could cause shadings at the edges of big sized sensors. Most c-mount lenses are able to illuminate a maximum image circle of

11 mm (2/3”), 16 mm (1”) or 22 mm

(4/3”) diameter only. The pco.edge cameras have a sensor diagonal from

18.8 to 21.8 mm, it follows that you have to use the ROI function for a shade less image while using the cmount adapter with the two smaller C-mount diameters.

51

A3 WATER COOLING OPTION PCO.AQUAMATIC II

A3.1 SYSTEM COMPONENTS

2

1

This is the re-cooling system for pco.edge cameras with watercooling.

3

5

6

4

1

2

Material No. Description

30108000211 pco.aquamatic II

30108000212

Connection Tube 5m PVC 3541-01 PCO

(with Colder NS212 fittings)

3

4

5

6

50402000055

20307500024

10307000226

10305000190

Power supply ETC 70-12 1.2m

Power cable

EDGE WAT camera cable 5m FGG-RG58-

NC3MX

Innovatec Protect IP 1L

The operation of the pco.aquamatic is simple and uncomplicated.

Normally no maintenance and nearly no attention are needed. Only the liquid level of the reservoir (water tank) should be controlled from time to time.

Only use Innovatec Protect IP for the pco.aquamatic! Do not use or add any other cooling liquid or normal water! If you need to add cooling liquid in order to maintain level in the tank, please contact

PCO for additional supply.

The cooling liquid will turn yellow after some hours of operation. This is normal and no sign of wear or malfunction. The optimum pH-value is between 8 and 9 (please check this value if you are concerned about the cooling liquid quality).

NOTE

The recommended service interval for the change of the cooling liquid is four years.

52

A3 WATER COOLING OPTION PCO.AQUAMATIC II

A3.2 FIRST TIME INSTALLATION

1

4

6

3

5

2

Please take care to situate the unit on a flat and firm surface. Do not cover the air intakes of the unit. Please ensure free airflow around the pco.aquamatic to ensure maximum cooling performance. All tubes and power cords need to run kink-free.

Before installation of the unit carefully read the

Innovatek Protect

IP safety datasheet (see Innovatek Website).

outlet flow

inlet flow

Please follow steps 1 – 6.

1

2

3

4 housing of the cooling unit only indicate flow direction. Either connection of the camera can be used for

in or out.

• Attach power connection.

• Open tank cover.

• Turn power switch to on position (I).

5

6 running, you have to constantly refill liquid.

• While the cooling liquid flows back to the reservoir make sure that the whole air escapes from the system – this takes a few minutes

(move hoses if necessary).

The cooling liquid reservoir (tank) is filled when liquid level is approximately 1-2 cm from the top of the tank. The integrated pump only works when the pump chamber is completely filled. To ensure this please move hoses or remove air by evacuating. Reservoir capacity is approximately 500 ml.

After

steps 1 – 6 are completed successfully the system is ready for

operation.

NOTE

The hose connectors are waterproof in not connected state.

Maybe they lose one drop of cooling liquid from time to time. You don’t have to empty the hoses while storing the camera system.

53

A3.3 OPERATION

First connect the power out of the cooling unit with the power in of the pco.edge camera by using the PCO WAT camera cable.

The cooling unit provides two operation modes.

Operation Mode on: the cooling unit is turned on permanently and provides the camera with power.

Your camera can be switchted on and off as needed.

Operation mode follow: the cooling unit will turn on when the camera is switched on and vice versa.

Error Codes

The coolant temperature sensor is located in the water tank.

Temperature

27°C

36°C

55°C

60°C

Action fan turns off fan turns on warning message error message

If a warning level is passed, the Power LED blinks slowly and the

Error LED displays the error code. If a failure level is passed, the

Power LED blinks fast and the Error LED shows the error code.

Error / Failure on

1Hz flash

2Hz flash

1Hz flash off one short flash

none

Warning when temperature at 55 °C

(also if sensor is defect or missing) one short flash Failure when temperature at 60 °C two short flashes

Fan speed

(also if a high deviation of the standard value is reached)

NOTE

The camera has its own protection circuit and will shut down

Peltier cooling automatically when the electronics temperature exceeds safety level. The camera itself will keep on running, but sensor temperature will increase. (valid for air and water cooling!)

If the camera and the water cooler have a different power supply, always first shut down camera and then the cooling system to avoid damages.

54

A3 WATER COOLING OPTION PCO.AQUAMATIC II

A3.4 DIMENSIONS

All dimensions are given in millimeter.

Weight: 4kg (completely filled cooling liquid tank)

General Information

You are not restricted to purchase the pco.aquamatic system - it is possible to use an own water cooling solution. A separate power supply will be provided to every pco.edge camera with water connectors. The hardware of the pco.edge cameras with USB 3.0

Interface is designed to work with or without a water cooling system, because a fan that provides adequate cooling is inside the camera anyway.

In case you use an own water cooling system, please make sure that the liquid you use to cool your camera is at all times NEVER below the dew point of the ambient temperature! In order to avoid any appearance of condensation, use a cooling liquid at room temperature. A liquid flow rate of 1–2 litres per minute is sufficient.

55

A4 INTERFACES

Two different interfaces are available for the pco.edge camera. This chapter describes the installation and configuration of USB and

Camera Link interfaces.

Furthermore USB range extension, Ring Buffer and FIFO Buffer are described in detail.

A4.1 CABLE POSSIBILITIES

NOTE

Please only use

Camera Link cable, which have been are

Overview of the maximum cable lengths

Camera Link base:

approved by PCO.

Otherwise full functionality is not guaranteed.

• standard:

• optional:

• maximum:

3 m

5 m

10 m (active cable)

• no cables are included in the camera price

USB 3.0:

• standard: 3 m

• cable and proper PCI express board included in the camera price

56

A4 INTERFACES

1

3

A4.2 CAMERA LINK

A4.2.1 INSTALLATION OF FRAME GRABBER

NOTE

Hardware Instal- lation must be performed by a technician, because high voltages can occur on the device. Always pull the main plug before opening the device.

Instructions for installing and testing the

SILICONSOFTWARE

microEnable IV (ME4) Camera Link grabber card.

Please install the latest silicon software runtime package before installing the hardware. (Download: www.pco.de/support)

When working on a 64 bit operating system, please make sure to install the proper (64 bit) runtime when also a 64 bit application will be operated. If the application is 32 bit, you need to install the 32 bit runtime accordingly.

1

Gigabit Ethernet board installed (standard), please deselect

Support for…

-

3

grabber card

4 The grabber card should be displayed within the device manager.

If the device is not shown this way, please reinstall the

SiliconSoftware device driver.

5

(see next page)

microDiagnostics.

2

4 5

57

5

How to use microDiagnostics Tool

1

2 4 3

Please verify the

1 which must be 1.40

(1.28 in hex) or above.

To test the board, select the board in

1

Test Board. 2

If the firmware is not

1.40 (1.28 in hex), please upgrade to the latest firmware. Select

3 Flash Board(s) , then select the grabber in the dialog and open the appropriate hap file.

Then click on

Yes when you’re asked to proceed. You must power cycle your computer after the upgrade.

In case the firmware version is ok, please click on

Test

Performance. 4

6

It is mandatory that

PCIe is Highspeed

5

Otherwise the board is probably not able to transfer the necessary data rate.

The transfer rates, as shown, should be

6 displayed.

For further information or problems with mainboards please contact our support section.

58

A4 INTERFACES

A4.2.2 IMAGE DATA FLOW

The

sCMOS image sensors provide an extremely high frame rate compared to other scientific image sensors. The high frame rate, along with the high dynamic, creates a large amount of data that must be handled and stored. The maximum data rate of the sCMOS image sensor is given by:

pco.edge 5.5 Camera Link:

[2560 * 2160 (pixel in 1 frame) * 2 Byte (= 16bit dynamic)] * 100

[frames/s] = 1.1 GB/s (pco.edge 5.5, Camera Link)

To handle this considerable amount of data, there are two options available:

Option 1: Real-time Recording to Computer

The pco.edge sCMOS camera series uses this option. For this purpose, the interface must be capable of transmitting data at the required speed. Interfaces such as GigE, USB 3.0 and Camera Link are not fast enough to transmit this data, which is delivered by the largest sCMOS image sensor. Nevertheless, there is a sophisticated solution that uses the Camera Link interface, which is integrated into the pco.edge camera. It is a fact of nature that light, itself, has its own noise component called photon or shot noise, which increases with light signal. In this approach, no compression is made in small signals, while for large signals a suitable compression is applied.

Since the introduced compression error is always smaller than the photon noise induced error, it is not seen and a so called

visual

lossless compression has been performed.

It can be shown that this is possible without any significant loss of information. Therefore, the calculation for the pco.edge camera at full speed and full frame has to be re-written:

[2560 * 2160 (pixel in 1 frame) * 1.5 Byte (= 12bit dynamic)] * 100

[frames/s] = 0.829 GB/s

The Camera Link interface can transmit this in real-time. In the future, recently introduced machine vision interfaces including CoaXPress and Camera Link HS are capable of transmitting sCMOS image data without any compression. A network type interface, 10GigE, is similar with respect to hardware to Camera Link HS, but incorporates all of the known GigE advantages and disadvantages. Here, the usual protocols are not favorable for image data transmission, and any network traffic can dramatically reduce available transmission speed.

The real-time data transmission into the computer allows for a variety of applications, since it is free from camera memory

limitations. Image data can be stored directly in the computer’s random access memory (RAM) up to more than 64 gigabyte. With an appropriate RAID system, the data can be stored directly to hard disks and there is no delay involved.

Option 2: Recording in the Camera

For high-speed imaging applications where data transfer rates are in the range of several GB/s it is accepted that the primary image memory (camRAM) must be located in the camera. Two examples of

59

such high-speed cameras are the pco.dimax with 36 GB of camRAM and the pco.dimax HD with 18 GB of camRAM memory. This allows for fast recording, but just up to the integrated memory limit. Before a second sequence can be recorded, one must endure the wait time until data is downloaded to computer storage. Therefore, this option can only be used for recording short sequences with enough time between each event to download the image data. This option is not necessary for the pco.edge as either with USB 3.0 or Camera Link interface, the image data will be recorded to the computer in realtime.

Memory Structure / Organization

As the memory is software-controlled in both options, it does not matter how the data is stored. The memory can be organized for ring buffer or FIFO recording, or for a specified number of images like a burst mode. The pco.edge camera enables all of these possibilities with the integrated dynamic link libraries and proprietary Camware application software. This allows the customer to select the memory structure and organization that is optimized for the application at hand.

Recommendations

In the following you can find two exemplary systems for RAID and

RAM storage options that have been successfully tested by pco for high performance and which we sell directly to our customers.

They only show a configuration example, for actual system configuration please call us directly.

RAID 0

SYSTEM

11x 2TB SATA 6 GB/s

16 GB DDR3 RAM

Intel® Core™ i7-3820 processor 3.6 GHz

GeForce GTS 640 2GB (NVIDIA CUDA)

Windows 7 Professional 64 bit

RAM

RECORD

ER

64 GB DDR3 RAM

Intel® Core™ i7-3820 processor 3.6 GHz

GeForce GTS 640 2GB (NVIDIA CUDA)

Windows 7 Professional 64 bit

NOTE

Items are exemplary and subject to change. Please contact PCO for recommendation of current systems and sell of these systems.

The use of normal consumer PCs is not recommended!

ASUS mainboards seem to fail generally during the HS-mode test and are not recommended.

Recommended minimum configuration (or better):

• CPU >= i7 2.5 GHz

• RAM >= 1066MHz, 8GB

• Mainboard Supermicro X9SRA (socket 2011)

60

A4 INTERFACES

A4.3 USB 3.0

A4.3.3 HARDWARE RECOMMENDATIONS

The pco USB 3.0 interface is based on the Cypress EZ-USB FX31 device and it is compatible to pco software such as Camware and

SDK. To run a pco USB 3.0 camera successfully the user should consider a number of important issues that are discussed in the following chapters.

Motherboard and Chipset Configuration

It is recommended to use a Motherboard with a state of the art USB

3.0 host controller for the onboard USB 3.0 ports. The latest generation of USB 3.0 xHCI host controllers manufactured by Intel,

Renesas (NEC), Fresco, Via Labs, ASMedia and Texas Instruments

Inc. (TI) are tested by Cypress Semiconductor Corporation. (see

Cypress EZ-USB® FX3TM SDK Release Notes, Version 1.2.3, chapter 1.3). Currently pco recommends only motherboards with the following onboard xHCI host controllers:

• Renesas/NEC

μPD720202 Host Controller

• Intel® USB 3.0 eXtensible Host Controller

If the motherboard does not have an onboard USB 3.0 port or the onboard USB 3.0 port does not work properly with a pco USB 3.0 camera, please use an independent PCIe USB 3.0 extension card.

In this case, pco recommends the following:

• DELOCK 89348 (U3-PCIE1XG202-10)

PCIe 1x (

μPD720202 host controller)

(This board is sent with your pco.edge USB 3.0 camera system as standard)

• DELOCK 89325 (U3X4-PCIE4XE101)

PCIe 4x (

μPD720202 host controller)

USB 3.0 extension card with xHCI4 host controller

The following companies manufacture USB 3.0 xHCI host controller:

• Renesas Electronics America Inc. (earlier NEC),

• Texas Instrument Inc.,

• VIA Labs Inc (Diamond),

• Fresco Logic Inc.,

• Intel Inc.,

• AMD Inc. and

• Etron Technology, Inc.

NOTE

To determine suitable manufacturers of the xHCI controller, please use the Windows Device Manager. For example, in

Windows 7 drop down the USB Controller entry and find the USB

3.0 host xHCI controller of the USB 3.0 device in the sub-tree.

Then right-click to open the properties dialog, click the details tab, and choose

Vendor in the properties pull-down box.

NOTE

USB 3.0 interface cards with controllers from Etron Technology,

Inc. have not yet been tested.

61

.

The following table gives an overview about tested and recommended system configurations or hardware components.

Component

Motherboard

USB 3.0 extension card

PCIe Slot

USB 3.0 Cable

USB 3.0 Hub

Recommendation

There is no recommendation for a manufacturer. But if an onboard USB 3.0 port is used, the Renesas´s xHCI host controller μPD720202 is recommended.

• DELOCK 89348 (U3-PCIE1XG202-10)

PCIe 1x ( μPD720202 host controller),

• DELOCK 89325 (U3X4-PCIE4XE101)

PCIe 4x ( μPD720202 host controller)

A PCIe 4x, 8x or 16x slot is recommended.

USB 3.0 cable included in the packaging of your pco

USB 3.0 camera

Not recommended

Operating System Windows XP 32 Bit, Win7 32 / 64 Bit, Win8 32 / 64 Bit

Multiple pco USB

3.0 cameras connected to a

PC

A separate DELOCK 89348 (U3-PCIE1XG202-10) extension card for each camera should be used to guaranty maximum data throughput of each camera.

FOL adapter See A4.3.7

Recommended Software Components

The pco USB 3.0 driver supports Windows 7/8 (x86 or x64) and

Windows XP x86 operating systems. Microsoft does not support USB

3.0 natively for Win 7 or older Windows versions. Therefore use the manufacturers’ supplied xHCI host controller driver. By contrast, in

Windows 8 Microsoft does provide a native generic xHCI Driver but pco recommends using the driver from the corresponding manufacturer of the xHCI host controller. Currently there are seven different vendors producing USB 3.0 host controllers:

• Renesas Electronics

America Inc. (earlier NEC)

• Texas Instrument Inc.

• Intel Inc.

• VIA Labs Inc (Diamond),

• Fresco Logic Inc.

• AMD Inc.

• Etron Technology, Inc.

Please make sure that the most recent driver from the manufacturer is installed before a pco USB 3.0 camera is connected to a USB 3.0 port of your computer. The appropriate driver for the USB 3.0 port could be loaded from the website of the manufacturer of the USB 3.0 host xHCI controller; or from the website of the USB 3.0 extension card vendor. The current driver of the extension card included in the packaging of your pco USB 3.0 camera is added to the

pco.USB

stick delivered with each pco.edge.

NOTE

To determine the driver currently in use for the xHCI controller, use the Windows Device Manager. For example in Win 7, drop down the USB-Controller entry and find the USB 3.0 host xHCI controller of the USB 3.0 device in the sub-tree. Then right-click to open the

Properties dialog, click the driver tab and choose driver details

Before the pco.edge USB 3.0 camera is connected to the computer

the pco USB 3.0 Driver has to be installed. (see 4.1) Please visit the

support section of our website to get the latest USB 3.0 camera driver.

62

A4 INTERFACES

A4.3.4 USB 3.0 FAQ

Can I use USB 2.0 PC components?

If the USB Port, the USB Cable, or the USB Hub does not support

USB 3.0, a USB 3.0 PCO camera will not operate properly. The camera will be recognized in the Device Manager as a USB 2.0 device. Operation of the PCO camera using Camware or one of the

PCO SDK camera related functions will fail and the following message box will appear: “Please make sure your camera is connected to a USB 3.0 port. If your camera is connected to a USB

3.0 port, please read the application note APL_USB3_xxx.pdf”.

How to identify the manufacturer of the USB 3.0 host controller?

If a driver for the onboard USB3.0 port or an independent PCIe USB

3.0 interface card is already installed, open the Windows Device

Manager. Then extend the ‘USB-Controller’ entry and find the USB

3.0 host controller of the specific USB 3.0 device in the sub-tree.

Right click to open the Properties dialog and then click the ‘details’ tab which will allow you to identify the correct manufacturer of the USB 3.0 controller.

Why can’t the PCO USB 3.0 camera be opened with Camware or the PCO SDK?

In case a PCO USB 3.0 camera is connected to your PC and the

Camware software starts in

Demo Mode; or, the Camera Open

Function of the PCO SDK returns an error, the explanation could be:

The PCO USB 3.0 driver is not installed to your system and the PCO

USB 3.0 device is listed as “unknown device” in the Device Manager.

If so, right click the

unknown device to open the properties dialog and install the PCO USB 3.0 driver. The driver can be downloaded from our website at http://www.pco.de/drivers/. If the PCO USB 3.0 driver is properly installed, there will be a

PCO cameras entry with a

‘pco.camera with USB 3.0’ device listed in the Device Manager. ––

Another issue could be that the USB 3.0 camera is not recognized by the OS and it does not appear in the Device Manger, meaning it is even not shown as ‘unknown device’. In this case please refer to

Chapter 4.1 and follow the instructions provided.

Why was the PCO USB 3.0 camera not recognized by the OS?

There could be a problem with the USB 3.0 xHCI host controller of the Motherboard or with the extension card installed in the PC.

Additionally, a bad USB 3.0 cable could be the problem.

How to increase the performance of the USB 3.0 data transfer?

To make sure the full data-throughput of the camera can be transferred to the PC’s RAM, it is recommended to use a PCIe 4x, 8x or 16x slot in the PC.

In the case of using Windows 8, poor performance could be caused by the Microsoft`s automatically installed generic xHCI USB 3.0 driver. In this case please load and install the driver of the manufacturer`s xHCI host controller.

63

If more than one PCO USB 3.0 camera is operated on a single PC, please note that extension cards have maximum bandwidths that with two or more cameras are being exceeded.

There exists a maximum bandwidth of an extension card, e.g. if the

DELOCK 89348 (U3-PCIE1XG202-10) PCIe 1x card is connected to a

PCIe 16x slot of the PC the effective bandwidth is about 360 MB/s. If two PCO USB 3.0 cameras are connected to both ports of the extension card, the accumulated data throughput of both cameras cannot exceed this 360 MB/s maximum. In this case it would help to use a second DELOCK 89348 (U3-PCIE1XG202-10) PCIe 1x card connected to a second PCIe 16x slot of the PC for the second PCO USB 3.0 camera.

How many PCO USB 3.0 cameras can be connected to and operated with one PC? How many PCO USB 3.0 cameras are supported by the PCO USB 3.0 driver?

The PCO USB 3.0 driver supports up to eight cameras using one PC

The USB 3.0 PCO Driver Installer does not work.

The installer will return a warning that the current OS is not supported. The installer supports the 32-bit and 64-bit versions of

Windows 7 and Windows 8.

If the installer file is executed and a no dialog window appears:

Please try to install the driver manually using the Windows Device

Manager. Contact our support team to get the driver files for manual installation.

The driver is correctly installed, but the USB 3.0 PCO camera does not appear in the Device Manager:

Make sure the PCO USB 3.0 camera is ready, the power switch is turned on, and the camera is connected to the PC.

Note that while installing the camera to the PC that during the USB

3.0 driver installation it could take a minute or more until the PCO

USB 3.0 device recognized and is available for use.

64

A4 INTERFACES

A4.3.5 INSTALLATION OF THE USB 3.0 CARD

NOTE

Hardware Instal- lation must be performed by a technician, because high voltages can occur on the device. Always pull the main plug before opening the device.

An external USB 3.0 Host Controller Card is enclosed to each pco.edge USB 3.0 camera.

Hardware Installation

First shut down your computer and install the USB 3.0 Host

Controller.

Driver Installation Instructions

• Within the provided installation files USB_HBA, open the folder

U3X4-PCIE4XE101, U3X4-PCIE1XE101, U3-PCIE1XG202.

• Open the subfolder Driver and run RENESAS-USB3-Host-Driver-

30230-setup.exe.

• If your current OS is Win7/8 and the User Account Control is enabled, a dialog could occur asking, if you wish to launch the setup: Accept with Yes.

1

3

extension card can be used.

2

3

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1

2

A4.3.6 RING BUFFER AND FIFO

Compared to the pco.edge cameras with Camera Link interface, the pco.edge with USB 3.0 interface has a small

internal buffer memory

integrated. Each recorded and read out image is directly transferred to this internal buffer and subsequently the image is transmitted to the computer, where it is either stored into the RAM or onto the hard disk drive. There are two transfer modes available: Ring Buffer and

FIFO buffer which can be accessed via SDK function:

PCO_SetStorageMode (see SDK manual).

From user perspective the main difference between both modes occurs, if for some reasons the USB 3.0 interface behaves

slower than its usual speed. In this case the Ring Buffer delivers faster the most recent image than the FIFO Buffer mode, which can be helpful for example if the image should be focused.

Ring Buffer (in camera memory-standard mode)

As described the read out and recorded image is directly transferred into the internal buffer memory. Subsequently the next image is stored to the next free space. For the transmission from the camera to the computer

always the

most recent image is used, which works fine in case the computer does not do anything else than transfer images to its

1 internal storage memory (RAM).

Now, if the transmission speed of the computer system is interrupted by e.g. software, it can happen that images get lost, because in Ring Buffer mode the most recent image has to be transmitted to the PC. In case of a delay in transmission, the image in the memory, which waits for transmission

will

lose its status as most recent image to the next image, and will be disregarded for transmission. Hence this image will be

irretrievably lost. Because Ring Buffer always transmits the most recent image and therefore the most real-time transmission, it is the ideal mode for e.g. adjustment of focus.

FIFO Buffer (in camera memory)

Like above the read out and the recorded image is directly transferred into the internal buffer memory. Subseqently the next image is stored to the next free space in memory.

If all the memory space is occupied the

camera stops

storing. In FIFO buffer mode like name stated, FIFO = first-in first-out, the first stored image is read-out from the memory first and subsequently transmitted to the computer. This as well works fine in case the computer does not do anything else than transfer of images to its internal storage memory.

Now, if the transmission speed of the computer system is interrupted by e.g. software, then still the first stored image is readout, and then the next image is read out and transmitted. However there could be a delay time (but need not). If now the slowdown causes the FIFO buffer to be completely filled, then the camera stops storing images and the next image will irretrievably lost as well, but in this mode it is

lost before storing. This mode is ideal for users who need maximum transmission security; an image loss is very unlikely.

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A5 BINNING IN CMOS SENSORS

A5 BINNING IN CMOS SENSORS

Binning describes the summation of single pixels to form larger pixels and thereby improve the signal-to-noise ratio (SNR).

NOTE

The current default binning mode is accumulation. Averaging is not available within Camware.

Binning in CCD image sensors

The term binning comes from scientific CCD image sensors. The prominent feature of charge-coupled-devices (CCD) is the lossless transport or shifting of charge packages until an amplifier circuit converts them into a voltage at the output, where the main readout noise contribution occurs. If charge packages from two or more pixels are added before they are read out (past the output amplifier) because of very low light signals; this process is called binning.

Since the signal is increased before it is read out, and the image sensor’s readout characteristics remain unchanged, binning improves the SNR, and the image sensor’s resolution is reduced. The lossless transport feature of CCD image sensors makes binning possible.

Binning in CMOS image sensors

In general, binning is not possible in CMOS image sensors because voltages are processed and no charges are transported. In each pixel, the light generated charges are converted into voltages with the readout noise contribution of these amplifiers. Therefore, as opposed to CCD image sensors, if these voltages were combined, the readout noise would also be combined, which would not have the same positive effect on the SNR.

Nevertheless, such a summation or even an averaging would be beneficial for the SNR, but with a smaller impact compared to CCD image sensors. Since such CMOS binning cannot be done within the image sensor, it either has to be done in the camera or in the computer.

CMOS binning 1 – accumulation: Pixel values can accumulate, causing an effective dynamic reduction or larger number formats, because the result might exceed the original format -- two times maximum 8 bit values will result in a 9 bit value. This will not be a problem if 12 bit values are accumulated and transported as 16 bit images. However, in the case of scientific CMOS, if 16 bit values are transmitted in 16 bit images, only two times 15 bit maximum values are allowed. The signal of the binned pixels will be accumulated, due to the properties of readout noise; the increase of the noise itself will only be as big as the square root of the number of binned pixels. The

SNR will improve and in addition, a reduction of the stored image data is achieved.

CMOS binning 2 – averaging: Pixel values can be averaged, which has the same effect on the SNR as accumulation due to the properties of noise. This would keep the image output format the same and would reduce the amount of image data that can be stored. When this type of CMOS binning is processed in the camera it is called hardware binning. This should not be confused with real binning in CCD image sensors, because the hardware that processes this binning is not much different from the hardware in computer processing. Therefore, the term

hardware binning may be misleading.

67

4

3

A6 LIGHTSHEET SCANNING MODE

Idle

The pco.edge

Lightsheet scanning mode is a special mode for lightsheet microscopy and only available for Camera Link versions via SDK (not in

Camware).

It is based on Rolling Shutter mode with

slow

scan rate (95.3MHz) and uses the readout mode

1

2

Standard line time value is

2

set from camera specific line time (see p.

6) up to

100 ms. An exposure area (between the orange bars, e.g. 3 possible for the user to select the number of lines and the exposure time per line.

This user-defined capability makes it possible to synchronize the pco.edge cameras with a lightsheet microscope that requires this method of camera exposure timing

Workflow (only PCO SDK)

• Set Rolling Shutter mode

• Set pixel rate to slow scan

• Set readout format E

1

• Set line timing on and the appropriate line time

3

2

• Set number of exposure lines

• Set number of delay lines 4

• Trigger mode and acquire mode and hwio settings are free to select by the user

SDK settings: (see SDK manual)

SC2_SDK_FUNC int WINAPI

PCO_SetCmosLineTiming

SC2_SDK_FUNC int WINAPI

PCO_SetCmosLineExposureDelay wParameter

1 wTimeBase (e.g. 2 dwLineTime (e.g. 40 ) dwExposureLines (e.g. dwDelayLines (e.g.

5 )

4

3

68

A7 IMAGE FILE FORMATS

A7 IMAGE FILE FORMATS

There are different file formats available for saving camera images with Camware.

b16

The b16 16 bit format is similar to the bmp format. However, 16 bit pixel values are used instead of 8 bit pixel values.

The file format consists either of a Basic Header (6 Long-parameter) or of an Extended Header (32 Long-parameter), the latter of which is optionally for additional information. There might follow a variable comment field (ASCII code). Finally, there is the actual data set that is saved linearly (as in the case of BMP files).

11

12

13

14

15

16

17

18



266

6

7

8

9

1

2

3

4

5

10

With the exception of the first value, all parameters are Long Integers

(4 Byte). The first 6 parameters must always exist. The rest of the parameters, as well as the comment field, are optional.

Parameter Function pco- file size header length image width image height extended header -1 (true), extended header follows color mode 0 = black/with camera, 1 = color camera b/w min b/w max black/white LUT-setting, minimum value black/white LUT-setting, maximum value b/w linlog the first 4 byte are the characters pco- file size in byte header size + comment filed in byte image width in pixel image height in pixel red min red max green min green max black/white LUT-setting,

0 = linear, 1 = logarithmic red LUT-setting, minimum value red LUT-setting, maximum value green LUT-setting, minimum value green LUT-setting, maximum value blue min blue max color linlog internal use blue LUT-setting, minimum value blue LUT-setting, maximum value color LUT-setting, 0 = linear, 1 = logarithmic

Comment file in ASCII characters with variable length of 0…XX.

The length of the comment filed must be documented in the header length field.

line 1, pixel 1

16 bit pixel data

value of the first pixel line 1, pixel 2

… value of the second pixel

We recommend that all images should be saved first in the b16

or TIFF format. The advantage is to have the b16 or tiff images available all the time. You will always have the maximum 16 bit information. Please note that not all image analysis programs can accommodate 16 bit data. The 8 bit format saves only the information displayed on the monitor screen. The 16 bit information will be lost and cannot be displayed later.

69

pcoraw

This 16 bit pco file format is based on the new BigTIFF format, thus allowing for file size > 4GB. A new pco proprietary compression scheme is added in case it is necessary.

Standard File Formats

TIFF

Tag Image File Format, version 6.0 and lower. There is a 16bit monochrome and color image format.

BMP

Windows Bitmap Format, b/w or color 8 bit format-images, which have been saved in BMP format can be loaded later only as 8 bit images, i.e. part of the original information (16 bit) is lost.

FTS

Flexible Image Transport System, Version 3.1. There is a 16 bit image format. The NASA/Science Office of Standards and Technology

(NOST) has defined this format. Some programs use the FIT extension for this format.

ASCII

16 bit format, some mathematical programs prefer ASCII data.

JPG

JPEG (named after the Joint Photographic Experts Group who created the standard) is a commonly used method of lossy compression for photographic images. The degree of compression can be adjusted, allowing a selectable tradeoff between storage size and image quality.

JP2

JPEG 2000 is a wavelet-based image compression standard and coding system. It was created by the Joint Photographic Experts

Group committee in the year 2000 with the intention of superseding their original discrete cosine transform-based JPEG standard

(created 1992).

AVI

Audio Video Interleave is a multimedia container format introduced by

Microsoft in November 1992 as part of its Video for Windows technology.

MPG

MPEG-1, similar to JPEG, is a standard for lossy compression of video and audio developed by the Moving Picture Experts Group

(MPEG).

WMV

Windows Media Video (WMV) is a compressed video compression format for several proprietary codecs developed by Microsoft. The original video format, known as WMV, was originally designed for

Internet streaming applications, as a competitor to RealVideo.

70

A8 CUSTOMER SERVICE

A8 CUSTOMER SERVICE

A8.1 SERVICE AND MAINTENANCE

Service

The camera is designed to operate with no need of special adjustments or periodic inspections.

Maintenance

Unplug the camera from any power supply before cleaning it.

Use a soft, dry cloth for cleaning the camera.

Do not use liquid cleaners or sprays.

The lens is best cleaned with pressurized air or with liquid cleaners such as pure alcohol or with special optical cleaners that are available at high quality photo stores.

Never use aggressive cleaning liquids such as gasoline, acetone, spirits or nitro cleanser.

Every time the input window is cleaned, there is the possibility of surface damage. Do not clean the input window unless it is absolutely necessary. Be careful and avoid scratches and damage to the input window surface.

Use a cotton swab dipped in pure alcohol or optical cleaning liquid and wipe only on the glass surface. Do not get any cleaning liquid on the metallic parts such as the lens thread, because tiny detached particles may scratch the surface.

Always store the camera with the protective cap or with a lens mounted to avoid dust and dirt on the input window!

Camera Disposal

The camera includes electronic devices, which can contain materials harmful to the environment. If the camera is to be discarded, please dispose of it in an environmentally responsible manner and use recycling facilities, where available.

71

A8.2 TROUBLE SHOOTING

If you have a question, which is not adequately addressed in this manual, please contact PCO or your local dealer.

To speed your request, we need the following information:

Short description of the problem Operating system (PC)

Description of your application Processor type (PC)

Camera settings

Type and version of camera

Memory

Graphic card

Software being used

Camera serial number

Graphic card setup

How to create a logfile:

• Enable log files: ? Help Menu → Logging

Enable Logfile

Camware will ask you to press NO to activate Logfiles after

restart of Camware

• Repeat the workflow which produces the faults.

• Open the Help Menu and click Support Mail (+ Support File) →

Camware will ask you:

Generate support file?

• Save this file (CWSupport.zip – don’t rename it) and send it to

PCO Support ([email protected])

• Or visit our website: http://www.pco.de/support/

Repair

Before sending the camera for repair, first contact your local dealer or PCO respectively.

When shipping the camera for repair, be certain to carefully pack the camera with proper shipping materials. If possible use the original packaging. Use the protection cap to protect the camera on the lens thread.

Firmware, Software and Driver Update

You will find all necessary software and drivers on the accompanying

USB storage device.

For the latest versions please check our website: http://www.pco.de/support/supportProducts/scmos-cameras/

72

A9 INDEX

A9 INDEX

NOTE:

The mentioned page is always the starting page of a chapter!

Key word

Acquire mode

Binning

C-mount

Cable lengths

Camware software

Camera Link

Connection options

Contrast settings (Convert control)

Demo mode (Camware)

Dimensions

Direct record to file

Double image mode

Driver installation

Global Reset

Global Shutter

Input / Output possibilities

Interfaces

Logfiles

Lightsheet scanning mode

Live preview

Recorder modes

Recorder

Recorder memory settings

Region of interest (Sensor)

Rescan for cameras

Ring Buffer

Rolling Shutter

Safety instructions

Save / Open / Print files

Sequence trigger mode

Short cuts

Support

Time stamp

Timing

Trigger Mode

USB

Water cooling

White Balance

Page

31

28, 67

51

35

40

49

41

56

13

57

33

30

9

26

23

33

56

45

68

14

31

37; 39

14

28

42

31

; 66

19

7

41

31

47

45

31

16; 19; 23;

26

16

61

52

47

Chapter

6.3.7

6.3.5, A5

A2

6.4; 6.6

6.2

6.3.5

6.7.3

6.3.7; A4.3.6

6.3.2

2

6.7.2

6.3.7

6.7.9

6.7.7

6.3.7

6.3.1; 6.3.2; 6.3.3;

6.3.4

6.3.1

A4.3

A3

6.7.9

6.3.3

6.3.8

A4

6.7.7

A6

6.2

6.3.7

A4.1

6

A4.2

6.3.8

6.3.9

6.7.1

A1

6.7.2

6.3.6

4.1

6.3.4

73

ABOUT PCO

pco.

In 1987, PCO was founded with the objective to develop and to produce specialized, fast and sensitive video camera systems, mainly for scientific applications. Meanwhile the product range of PCO cameras covers digital camera systems with high dynamic range, high resolution, high-speed and low noise, which are sold in the scientific and industrial market all over the world.

Currently PCO is one of the leading manufacturers of scientific cameras. Worldwide representatives, together with our own sales department and technical support assure that we keep in touch with our customers and their needs. The actual wide range of specialized camera systems is the result of technical challenge and product specific know-how. A design according to advanced techniques, a high standard of production and strict quality controls guarantee a reliable operation of the cameras. Our own developments in conjunction with an excellent contact to leading manufacturers of image sensors ensure our access to state-of-the-art CCD- and

CMOS-technology for our cameras.

Since 2001, PCO is located in its own facility building in Kelheim at the shore of the beautiful and international river Danube. Here in the county Bavaria, which is well known for its excellent support and conditions for high technology companies, we share the benefits of the simple access to high performance products and services in the surrounding area.

Kelheim itself is a historical town, first documented in 866. The small city is founded at the confluence of the Danube and the Altmühl, which has been converted into the Rhine-Main-Danube bypass channel for water transport. Located in Danube-valley, it is the heart of a beautiful river and forest covered lime plateau landscape. It´s landmark, the Hall of Liberation, was built by Ludwig I. in 1863 on the

Mount Michael and is visible from all over the city and valley. The beautiful Danube-Gorge, which is protected as natural monument since 1840, is located between Kelheim and the famous abbey

Weltenburg.

74

pco.

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