Ettan IPGphor 3 Operating Instructions

Ettan IPGphor 3 Operating Instructions
Ettan™ IPGphor™ 3
Operating Instructions
Original instructions
Page intentionally left blank
Table of Contents
Table of Contents
1
Introduction ..........................................................................................................
1.1
1.2
1.3
1.4
2
3
4
5
6
7
9
12
Safety instructions ...............................................................................................
13
2.1
2.2
2.3
2.4
2.5
Safety precautions ...............................................................................................................................
Labels .........................................................................................................................................................
Emergency procedures ......................................................................................................................
Recycling information .........................................................................................................................
Declaration of Hazardous Substances (DoHS) ........................................................................
14
20
22
24
25
System description ..............................................................................................
27
3.1
3.2
3.3
3.4
Illustrations of Ettan IPGphor 3 ......................................................................................................
Ettan IPGphor 3 instrument .............................................................................................................
Ettan IPGphor 3 Control Software .................................................................................................
Strip holders ............................................................................................................................................
28
32
34
35
Installation ............................................................................................................
37
4.1
4.2
4.3
4.4
Site requirements ..................................................................................................................................
Unpacking and transport ..................................................................................................................
Setup ...........................................................................................................................................................
Software installation for isoelectric focusing process .........................................................
38
40
41
45
Operation ..............................................................................................................
46
5.1
47
Preparations before start ..................................................................................................................
5.1.1
5.1.2
5.2
5.3
Use the strip holders .....................................................................................................................
Use the Ettan IPGphor Cup Loading Manifold ...................................................................
48
59
Start up the instrument .....................................................................................................................
Perform a run from the control panel .........................................................................................
66
68
5.3.1
5.3.2
5.3.3
5.3.4
5.3.5
5.4
Ettan IPGphor 3 control panel functionality .......................................................................
Protocol parameters .....................................................................................................................
Set protocol parameters .............................................................................................................
Isoelectric focusing from the control panel ........................................................................
Pause or stop a protocol .............................................................................................................
69
73
75
83
89
Run a pre-programmed protocol from the PC ........................................................................
90
5.4.1
5.4.2
6
5
About this manual ................................................................................................................................
Important user information .............................................................................................................
Regulatory information ......................................................................................................................
Associated documentation ..............................................................................................................
Setting up a run ..............................................................................................................................
Perform a run ..................................................................................................................................
91
97
Maintenance .........................................................................................................
102
6.1
6.2
103
107
Cleaning Ettan IPGphor 3 ..................................................................................................................
Replace fuses ..........................................................................................................................................
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
3
Table of Contents
7
Troubleshooting ...................................................................................................
109
8
Reference information ........................................................................................
111
8.1
8.2
8.3
Health and Safety Declaration Form ...........................................................................................
Technical specifications .....................................................................................................................
Ordering information ..........................................................................................................................
112
114
116
Index .......................................................................................................................
118
4
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
1 Introduction
1
Introduction
About this chapter
This chapter contains information about this Operating Instruction, important user information, regulatory information and lists of associated documentation.
In this chapter
Section
See page
1.1 About this manual
6
1.2 Important user information
7
1.3 Regulatory information
9
1.4 Associated documentation
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
12
5
1 Introduction
1.1 About this manual
1.1
About this manual
Purpose of this Operating
Instruction
The Operating Instruction provides you with the instructions needed to install, operate
and maintain theEttan IPGphor 3 Isoelectric Focusing System in a safe way.
Scope of this manual
The document provides you with the instructions needed to use the Ettan IPGphor 3
Isoelectric Focusing System together with Ettan IPGphor 3.
Typographical conventions
Software items are identified in the text by bold italic text. A colon separates menu levels,
thus File:Open refers to the Open command in the File menu.
Hardware items are identified in the text by bold text (e.g., Power switch).
6
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
1 Introduction
1.2 Important user information
1.2
Important user information
Read this before operating
Ettan IPGphor 3
All users must read the entire Ettan IPGphor 3 Operating Instructions before installing,
operating or maintaining the instrument. Always keep the Operating Instructions at
hand when using Ettan IPGphor 3.
Do not operate Ettan IPGphor 3 in any other way than described in the user documentation. If you do, you may be exposed to hazards that can lead to personal injury and you
may cause damage to the equipment.
Intended use
Ettan IPGphor 3 Isoelectric Focusing System performs the first-dimension isoelectric focusing (IEF) step in 2-dimensional (2-D) electrophoresis. The system comprises GE's
Ettan IPGphor 3, combined with separate strip holders or a manifold. Two types of
manifolds are available; the ceramic manifold and the light manifold. The IEF separation
protocols are programmed, run and controlled from the Ettan IPGphor 3 control panel
or from a PC running the Ettan IPGphor 3 Control Software.
Ettan IPGphor 3 is intended to be used as a laboratory unit for research purposes.
Ettan IPGphor 3 shall not be used in any clinical procedures, or for diagnostic purposes.
For larger sample volumes the Ettan IPGphor 3 Cup Loading Manifold facilitates paper
bridge loading and in gel rehydration loading, for more information see Ettan IPGphor
Cup Loading Manifold User Manual.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
7
1 Introduction
1.2 Important user information
Safety notices
This user documentation contains WARNINGS, CAUTIONS and NOTICES concerning the
safe use of the product. See definitions below.
Warnings
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
Cautions
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to proceed until all stated conditions are met and clearly understood.
Notices
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
Notes and tips
8
Note:
A Note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip:
A tip contains useful information that can improve or optimize your procedures.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
1 Introduction
1.3 Regulatory information
1.3
Regulatory information
Introduction
This section lists the directives and standards that are fulfilled by Ettan IPGphor 3
Isoelectric Focusing System.
Manufacturing information
The table below summarizes the required manufacturing information. For further information, see the EU Declaration of Conformity (DoC) document.
Requirement
Content
Name and address of manufacturer
GE Healthcare Bio-Sciences AB,
Björkgatan 30, SE 751 84 Uppsala, Sweden
Conformity with EU Directives
This product complies with the European directives listed in the table, by fulfilling the
corresponding harmonized standards.
Directive
Title
2006/42/EC
Machinery Directive (MD)
2004/108/EC
Electromagnetic Compatibility (EMC) Directive
2006/95/EC
Low Voltage Directive (LVD)
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
9
1 Introduction
1.3 Regulatory information
CE marking
The CE marking and the corresponding EU Declaration of Conformity is valid for the instrument when it is:
•
used as a stand-alone unit, or
•
connected to other products recommended or described in the user documentation,
and
•
used in the same state as it was delivered from GE, except for alterations described
in the user documentation.
International standards
Standard
Description
Notes
EN/ IEC 61010-1,
CAN/CSA-C22.2 No.
61010-1
Safety requirements for
electrical equipment for
measurement, control and
laboratory use
EN standard is harmonized with EU directive
2006/95/EC
EN/IEC 61326-1 (Emission
according to CISPR 11,
Group 1, class A)
Electrical equipment for
measurement, control and
laboratory use - EMC requirements
EN standard is harmonized with EU directive
2004/108/EC
EN ISO 12100
Safety of machinery. General principles for design.
Risk assessment and risk
reduction.
EN ISO standard is harmonized with EU directive
2006/42/EC
FCC compliance
This device complies with part 15 of the FCC Rules. Operation is subject to the following
two conditions: (1) This device may not cause harmful interference, and (2) this device
must accept any interference received, including interference that may cause undesired
operation.
Note:
10
The user is cautioned that any changes or modifications not expressly approved
by GE could void the user’s authority to operate the equipment.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
1 Introduction
1.3 Regulatory information
This equipment has been tested and found to comply with the limits for a Class A digital
device, pursuant to part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a
commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may
cause harmful interference to radio communications. Operation of this equipment in a
residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense.
Regulatory compliance of
connected equipment
Any equipment connected to Ettan IPGphor 3 Isoelectric Focusing System should meet
the safety requirements of EN 61010-1/IEC 61010-1 or relevant harmonized standards.
Within the European Union, connected equipment must be CE-marked.
Environmental conformity
This product conforms to the following environmental requirements.
Requirement
Title
2011/65/EU
Restriction of Hazardous Substances (RoHS) Directive
2012/19/EU
Waste Electrical and Electronic Equipment (WEEE) Directive
ACPEIP
Administration on the Control of Pollution Caused by Electronic
Information Products, China Restriction of Hazardous Substances (RoHS)
Regulation (EC)
No 1907/2006
Registration, Evaluation, Authorization and restriction of
CHemicals (REACH)
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
11
1 Introduction
1.4 Associated documentation
1.4
Associated documentation
Introduction
This section describes associated documentation and how to find related literature that
can be downloaded or ordered from GE.
12
•
Ettan IPGphor Cup Loading Manifold User Manual contains instructions for first-dimension isoelectric focusing of proteins on IPG strips.
•
Ettan IPGphor 3 Control Software User Manual
•
2-D Electrophoresis, principles and methods guide
•
Instructions attached to each package of Immobiline™ DryStrip.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
2 Safety instructions
2
Safety instructions
About this chapter
This chapter describes safety precautions and emergency shutdown procedures for the
Ettan IPGphor 3 Isoelectric Focusing System. The labels on the system and information
regarding recycling are also described.
In this chapter
Section
See page
2.1 Safety precautions
14
2.2 Labels
20
2.3 Emergency procedures
22
2.4 Recycling information
24
2.5 Declaration of Hazardous Substances (DoHS)
25
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
13
2 Safety instructions
2.1 Safety precautions
2.1
Safety precautions
Introduction
Before operating the instrument, you must be aware of the hazards described in the
user documentation. Follow the instructions provided to avoid personal injury or damage
to the equipment.
The safety precautions in this section are grouped into the following categories:
•
General precautions, on page 14
•
Personal protection, on page 15
•
Using flammable liquids, on page 16
•
Maintenance, on page 19
•
System operation, on page 17
•
Maintenance, on page 19
General precautions
WARNING
Perform a risk assessment for any risks due to the process or process environment. Evaluate the effect the system and the processes
where it is used have to the classification of the hazardous area.
The process might cause the area to increase or the zone classification to change. Implement the risk reduction measures needed,
including use of personal protection equipment.
WARNING
The customer must make sure that all installation, maintenance,
operation and inspection is carried out by qualified personnel who
are adequately trained, understand and adhere to local regulations
and the operating instructions, and have a thorough knowledge
of the product and the entire process.
14
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
2 Safety instructions
2.1 Safety precautions
WARNING
Do not operate Ettan IPGphor 3 in any other way than described
in the Operating Instructions.
WARNING
Operation and user maintenance of Ettan IPGphor 3 should be
performed by properly trained personnel only.
WARNING
Only use spare parts and accessories that are approved or supplied
by GE for maintaining or servicing the system.
Personal protection
WARNING
Always use appropriate personal protective equipment during installation, operation, inspection, maintenance and cleaning of
Ettan IPGphor 3.
WARNING
When using hazardous chemical and biological agents, take all
suitable protective measures, such as wearing protective glasses,
masks and gloves resistant to the substances used and protect
self from hazardous substances that are used. Follow local and/or
national regulations for safe operation and maintenance of the
system.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
15
2 Safety instructions
2.1 Safety precautions
WARNING
The operator has to take all necessary actions to avoid spreading
hazardous biological agents in the vicinity of the instrument. The
facility should comply with the national code of practice for
biosafety.
WARNING
Ettan IPGphor 3 is a Class A product. In a domestic environment,
it might cause radio interference, in which case the user might be
required to take appropriate measures.
Using flammable liquids
WARNING
Before starting the system, make sure that there is no leakage.
WARNING
A fume hood or similar ventilation system shall be installed when
flammable or noxious substances are used.
Installation
WARNING
Make sure that the supply voltage at the wall outlet corresponds
to the marking on the instrument, before connecting the power
cord.
WARNING
The Ettan IPGphor 3 must always be connected to a grounded
power outlet.
16
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
2 Safety instructions
2.1 Safety precautions
WARNING
Only use power cords delivered or approved by GE.
WARNING
Do not block the rear and side panel of Ettan IPGphor 3. The power
switch must always be easy to access. The power cord must always
be easy to disconnect.
NOTICE
Any computer used with the equipment shall comply with IEC 60950
and be installed and used according to the manufacturer's instructions.
System operation
WARNING
The Ettan IPGphor 3 is capable of producing thousands of volts.
Before operating the unit, read and fully understand the
Ettan IPGphor 3 operating instructions and warnings.
WARNING
Check that the high voltage is turned off before opening the safety
lid. The HV ON indicator on the front panel should not light.
WARNING
Ettan IPGphor 3 must never be used if any safety functions are out
of order. Contact your GEservice operator for more information.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
17
2 Safety instructions
2.1 Safety precautions
WARNING
Ettan IPGphor 3 is a high voltage instrument that can cause fatal
electrical shock if the safety features are disabled. The safety lid
must be securely closed before starting a protocol.
WARNING
In a situation where there is a risk of injury, turn off the instrument
by switching the mains power switch (located on the rear panel)
to position 0. Malfunctions must be rectified before Ettan IPGphor 3
Isoelectric Focusing System is restarted.
WARNING
The vents at the front, back, and bottom of Ettan IPGphor 3 must
not be obstructed. Air must be free to circulate for the cooling apparatus to function correctly. There should be a minimum of 10
cm unobstructed space behind the rear panel of the instrument.
WARNING
Avoid spilling liquids on the body of the instrument. If large volumes
of liquid have penetrated the casing of the instrument and come
into contact with the electrical components, immediately switch
off the instrument and contact an authorized service technician.
CAUTION
Always wear protective gloves when working with rehydration solution.
CAUTION
Always wear gloves when handling IPG strips and the equipment
that comes in contact with them. This will help minimize protein
contamination which can result in artifactual spots in stained 2-D
gel patterns.
18
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
2 Safety instructions
2.1 Safety precautions
CAUTION
The safety lid must be properly closed before power is applied.
Maintenance
WARNING
Disconnect power. Always disconnect power from the instrument
before performing any maintenance task.
WARNING
Ettan IPGphor 3 covers must not be opened by the user. It contains
electrical circuits which can give a lethal electric shock. Service
and planned maintenance should be performed by personnel authorized by GE.
WARNING
Do not remove the main cover. There are no userserviceable
components inside, and you can be exposed to high voltage.
WARNING
Always disconnect power from the instrument and detach the
power cord before replacing fuses.
CAUTION
Do not use any organic solvents during cleaning.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
19
2 Safety instructions
2.2 Labels
2.2
Labels
Introduction
This section describes the system label and the safety labels on Ettan IPGphor 3.
System label illustration
The illustration below shows an example of a system label.
Sample
XX-XXXX-XX
XXXXXTM
Code no: XXXXXXXX
Serial no: XXXXXXX
Mfg Year: 2014
Voltage: IXI-XXX/XXX-XXX VAC
Frequency: XX-XX Hz
Max Power: XXX VA
Fuse: XX X XXAL 250 V
Protection Class: XXXX
4001767
Made in Sweden
GE Healthcare Bio-Sciences AB
751 84 Uppsala Sweden
Conforms to ANSI/UL Std. 61010-1. Cert. to CAN/CSA Std. C22.2 No. 61010-1.
System label description
20
Label
Description
Product name
Name of product
Code no
Instrument Assembly Number
Serial no
Instrument serial number
Mfg Year
Manufacturing year
Voltage
Voltage
Frequency
Frequency
Max Power
Max Power rating
Fuse
Fuse
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
2 Safety instructions
2.2 Labels
Label
Description
Warning! Read the user documentation before using the system. Do not open any covers or replace parts unless specifically
stated in the user documentation.
This symbol indicates that the waste of electrical and electronic
equipment must not be disposed as unsorted municipal waste
and must be collected separately. Please contact an authorized
representative of the manufacturer for information concerning
the decommissioning of equipment.
This symbol indicates that the product contains hazardous
materials in excess of the limits established by the Chinese
standard SJ/T11363-2006 Requirements for Concentration
Limits for Certain Hazardous Substances in Electronics.
The system complies with applicable European directives. Refer
to Conformity with EU Directives, on page 9.
The system complies with the requirements for electromagnetic
compliance (EMC) in Australia and New Zealand.
This symbol indicates that Ettan IPGphor has been certified by
a Nationally Recognized Testing Laboratory (NRTL). NRTL means
an organization, which is recognized by the US Occupational
Safety and Health Administration (OSHA) as meeting the legal
requirements of Title 29 of the Code of Federal Regulations (29
CFR), Part 1910.7.
Safety label description
Safety labels (see figure below) are attached to both the light protective cover and the
transparent lid of the safety lid. The safety label warns the user of risk for personal injury.
Do not proceed until the instructions are clearly understood and all stated conditions
are met.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
21
2 Safety instructions
2.3 Emergency procedures
2.3
Emergency procedures
Introduction
This section describes how to perform an emergency shutdown of Ettan IPGphor 3 and
the result in the event of power failure.
Emergency shutdown
Switch off power to the instrument by pressing the Power switch to the 0 position (1). If
required, disconnect the mains power cord (2). The run is interrupted immediately.
This will ensure that the power is cut immediately even if an uninterruptible power supply
(UPS) is used.
1
/
2
Power failure
In the event of system shutdown due to power failure, emergency stop or process interruption, malfunctions must be rectified before the Ettan IPGphor 3 is restarted.
The result of a power failure depends on which unit is affected.
22
Power failure to...
will result in...
Ettan IPGphor 3 instrument
•
Any experiments that are running will stop.
•
The time for the stop will be logged in the instrument.
•
The run is interrupted in an undefined state.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
2 Safety instructions
2.3 Emergency procedures
Power failure to...
will result in...
Computer
•
The Ettan IPGphor computer shuts down.
•
Any running experiments will be continued and completed.
•
Scanned images will be saved and uploaded to the
computer when computer contact has been re-established.
Note:
A UPS can eliminate data loss during and after a power failure, and allow time
for a controlled shut-down of Ettan IPGphor 3.
Restart after emergency
shutdown/power failure
When the power returns to normal no experiments that were running at the time of
emergency shutdown will be continued. Proceed as follows:
Step
Action
1
Start the instrument, see Turn on power and run diagnostic program, on
page 66.
2
Restart the software.
When the system is connected, the log event will be stored in the system
log.
3
If you wish to continue a run, set up and run a new experiment with the
appropriate/remaining steps to complete the run in the best way.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
23
2 Safety instructions
2.4 Recycling information
2.4
Recycling information
Introduction
This section contains information about the decommissioning of Ettan IPGphor 3.
Decontamination
Ettan IPGphor 3 shall be decontaminated before decommissioning and all local regulations shall be followed with regard to scrapping of the equipment.
Disposal, general instructions
When taking Ettan IPGphor 3 out of service, the different materials must be separated
and recycled according to national and local environmental regulations.
Recycling of hazardous
substances
Ettan IPGphor 3 contains hazardous substances. Detailed information is available from
your GE representative.
Disposal of electrical
components
Waste of electrical and electronic equipment must not be disposed as unsorted municipal
waste and must be collected separately. Please contact an authorized representative
of the manufacturer for information concerning the decommissioning of equipment.
24
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
2 Safety instructions
2.5 Declaration of Hazardous Substances (DoHS)
2.5
Declaration of Hazardous Substances (DoHS)
根据SJ/T11364-2006《电子信息产品污染控制标识要求》特提供如下有关污染 控制方面的信息。
The following product pollution control information is provided according to SJ/T11364-2006 Marking
for Control of Pollution caused by Electronic Information Products.
电子信息产品污染控制标志说明
Explanation of Pollution Control Label
该标志表明本产品含有超过SJ/T11363-2006《电子信息产品中有毒有害物质的限
量要求》中限量的有毒有害物质。标志中的数字为本产品的环保使用期,表明本
产品在正常使用的条件下,有毒有害物质不会发生外泄或突变,用户使用本产品
不会对环境造成严重污染或对其人身、财产造成严重损害的期限。单位为年。
为保证所申明的环保使用期限,应按产品手册中所规定的环境条件和方法进行正
常使用,并严格遵守产品维修手册中规定的期维修和保养要求。
产品中的消耗件和某些零部件可能有其单独的环保使用期限标志,并且其环保使
用期限有可能比整个产品本身的环保使用期限短。应到期按产品维修程序更换那
些消耗件和零部件,以保证所申明的整个产品的环保使用期限。
本产品在使用寿命结束时不可作为普通生活垃圾处理,应被单独收集妥善处理。
This symbol indicates the product contains hazardous materials in excess of the limits
established by the Chinese standard SJ/T11363-2006 Requirements for Concentration
Limits for Certain Hazardous Substances in Electronic Information Products. The
number in the symbol is the Environment-friendly Use Period (EFUP), which indicates
the period during which the toxic or hazardous substances or elements contained in
electronic information products will not leak or mutate under normal operating conditions so that the use of such electronic information products will not result in any
severe environmental pollution, any bodily injury or damage to any assets. The unit
of the period is “Year”.
In order to maintain the declared EFUP, the product shall be operated normally according to the instructions and environmental conditions as defined in the product
manual, and periodic maintenance schedules specified in Product Maintenance Procedures shall be followed strictly.
Consumables or certain parts may have their own label with an EFUP value less than
the product. Periodic replacement of those consumables or parts to maintain the
declared EFUP shall be done in accordance with the Product Maintenance Procedures.
This product must not be disposed of as unsorted municipal waste, and must be
collected separately and handled properly after decommissioning.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
25
2 Safety instructions
2.5 Declaration of Hazardous Substances (DoHS)
有毒有害物质或元素的名称及含量
Name and Concentration of Hazardous Substances
产品中有毒有害物质或元素的名称及含量
Table of Hazardous Substances’ Name and Concentration
部件名称
有毒有害物质或元素
Component name
Hazardous substance
28-9269-30
铅
汞
镉
六价铬
多溴联苯
多溴二苯醚
Pb
Hg
Cd
Cr6+
PBB
PBDE
X
0
0
0
0
0
0:
表示该有毒有害物质在该部件所有均质材料中的含量均在SJ/T11363-2006 标准规定的限
量要 求以下
X:
表示该有毒有害物质至少在该部件的某一均质材料中的含量超出SJ/T11363-2006 标准规
定的限量要求
•
此表所列数据为发布时所能获得的最佳信息
0:
Indicates that this toxic or hazardous substance contained in all of the homogeneous materials for this part is below the limit requirement in SJ/T11363-2006.
X:
Indicates that this toxic or hazardous substance contained in at least one of the homogeneous materials used for this part is above the limit requirement in SJ/T11363-2006.
•
Data listed in the table represents best information available at the time of publication.
26
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
3 System description
3
System description
About this chapter
This section provides a description of Ettan IPGphor 3 Isoelectric Focusing System and
an overview of its components.
In this chapter
This chapter contains the following sections:
Section
See page
3.1 Illustrations of Ettan IPGphor 3
28
3.2 Ettan IPGphor 3 instrument
32
3.3 Ettan IPGphor 3 Control Software
34
3.4 Strip holders
35
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
27
3 System description
3.1 Illustrations of Ettan IPGphor 3
3.1
Illustrations of Ettan IPGphor 3
Ettan IPGphor 3
Isoelectric Focusing System
1
2
Part
Function
1
Ettan IPGphor 3 Control Software
2
Ettan IPGphor 3 instrument
Note:
28
Ettan IPGphor 3 instrument is used together with separate strip holders or a
manifold. Two types of manifolds are available; the ceramic manifold and the
light manifold.
Ettan IPGphor 3 Isoelectric Focusing System also includes Immobiline DryStrip
gel strips, which contain an immobilized pH gradient (IPG).
For more information about the manifolds, see Ettan IPGphor Cup Loading
Manifold User Manual.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
3 System description
3.1 Illustrations of Ettan IPGphor 3
Ettan IPGphor 3 platform
1Safety lid
(anode)
2Positive
electrode area
Negative (cathode)
area
3electrod
4Control panel
Part
Function
1
Safety lid
2
Positive (anode) electrode area
3
Negative (cathode) electrode area
4
Control panel
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
29
3 System description
3.1 Illustrations of Ettan IPGphor 3
Ettan IPGphor 3 control panel
Ettan IPGphor 3 is controlled by a 7-key membrane keypad. The instrument status is indicated on the LCD screen.
1
2
3
9
30
Part
Function
1
START button
2
STOP button
3
LCD screen (Main screen)
4
Left arrow button
5
Up arrow button
6
Right arrow button
7
Down arrow button
8
EDIT button
9
HV ON indicator lamp
4
5
7
6
8
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
3 System description
3.1 Illustrations of Ettan IPGphor 3
Ettan IPGphor 3 rear panel
Electrical and communication connections are placed on the rear panel of the
Ettan IPGphor 3.
1
/
2
3
4
Part
Function
1
Power switch
2
Fuse holder
3
RS232 for connection to computer
4
Power inlet
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
31
3 System description
3.2 Ettan IPGphor 3 instrument
3.2
Ettan IPGphor 3 instrument
Platform
The platform accommodates one manifold for the first dimension isoelectric focusing
(IEF) run. Up to 12 fixed-length strip holders can be placed on the platform for rehydration
and IEF. The embedded electrode areas provide the electrical connection between strip
holder or manifold electrodes and an integrated high-voltage DC power supply delivering
up to 10 kV. Platform temperature is controlled by Peltier thermoelectric modules.
Both electrode areas are gold-plated copper. Placing a manifold or a strip holder so that
it bridges both electrode areas completes the electrical circuit (once the safety lid is in
position).
Protective cover
When analyzing proteins labeled with light sensitive stains like CyDye™, a light protective
cover is used. The cover is applied by magnetic force but is easy to partially remove for
checking the isoelectric focusing procedure, especially the migration of bromo-phenolblue at start.
The voltage is cutoff when the safety lid is opened.
32
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
3 System description
3.2 Ettan IPGphor 3 instrument
Lid adapter for strip holders
When using Ettan IPGphor 3 standard strip holders a lid adapter must be used to apply
the correct amount of pressure to the lids of the Ettan IPGphor 3 strip holders.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
33
3 System description
3.3 Ettan IPGphor 3 Control Software
3.3
Ettan IPGphor 3 Control Software
Control software
Ettan IPGphor 3 Control Software is used to control the Ettan IPGphor 3
Isoelectric Focusing System. The software provides data presentation, data storage, and
protocol handling. The PC is connected to the Ettan IPGphor 3 by a serial cable or USB
converter.
Ettan IPGphor 3 Control Software controls up to four Ettan IPGphor 3 systems at one
time, each running a different set of run parameters.
The software allows programming and recommended protocols are generated by providing instrument configuration, IPG strip length and pH gradient.
The software records the run parameters over time and presents data as graphs and
log files. Data is saved or can be exported to Microsoft Excel.
34
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
3 System description
3.4 Strip holders
3.4
Strip holders
Two models of strip manifolds
IPG strip manifolds are available in two models:
•
One produced from a ceramic material providing greater temperature uniformity
•
One produced from a light polymeric material for less critical experimentation
Both are designed for analytical analysis using cup application and preparative runs
applying sample to the gel during the rehydration step or by paper bridge loading.
Strip holder
The strip holders are made of aluminum oxide ceramic with electrodes of platinum on
titanium. The ceramic is very brittle so the holders are fragile and must be handled with
care.
The ceramic is treated with a special coating to minimize protein binding and must be
cleaned only with the Ettan IPGphor Cleaning Solution supplied to retain that coating.
Each fixed-length strip holder base holds a single IPG strip throughout rehydration and
isoelectric focusing. A matching length holder is available for each size strip. All strips
(and holders) used at any one time must be of the same length.
Five strip holder lengths are available, 7, 11, 13, 18, and 24 cm, one for each IPG strip
length and a number of pH ranges, several linear and non-linear. Refer to Section 8.3
Ordering information, on page 116.
24 cm
18 cm
13 cm
11 cm
7 cm
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
35
3 System description
3.4 Strip holders
Part
Function
Sample application wells
Each of the two wells can contain to 15 μl of sample (7.5 μl on either side of the strip) in excess of the rehydration volume (i.e. 30
μl maximum for both wells). Introduce the sample through the
wells if it is not included in the rehydration solution.
Cover
The cover is made of acrylic and protects the IPG strip during rehydration. The shape of the underside of the cover ensures electrical continuity during IEF by pressing the strip against the electrode
while allowing electrolysis gases to escape. Rehydration and separation progress can be monitored visually through the clear
cover.
Strip holder cover
The cover is made of acrylic and protects the IPG strip during rehydration. The shape of
the underside of the cover ensures electrical continuity during IEF by pressing the strip
against the electrode while allowing electrolysis gases to escape.
Rehydration and separation progress can be monitored visually through the transparent
cover.
2
1
3
4
36
Part
Function
1
Pressure block
2
Cover
3
Base
4
Sample application walls
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
4 Installation
4
Installation
Introduction
This chapter provides information about safe installation of Ettan IPGphor 3
Isoelectric Focusing System.
In this chapter
This chapter contains the following sections:
Section
See page
4.1 Site requirements
38
4.2 Unpacking and transport
40
4.3 Setup
41
4.4 Software installation for isoelectric focusing process
45
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
37
4 Installation
4.1 Site requirements
4.1
Site requirements
Space requirements
Parameter
Specification
Minimum bench area ( w × d)
480 × 670 mm
Dimensions and weight of
Ettan IPGphor 3
Parameter
Specification
Dimensions (h × w × d)
160 × 278 × 471 mm
Weight
8.3 kg
Operating environment
1
38
Parameter
Specification
Operation site
Indoor use
Altitude
Maximum 2000 m
Ambient temperature
15°C to 32°C
Relative humidity
0% to 70% 1
Pollution degree
2
Noise emission
<80 dB A
High relative humidity may result in condensation forming on the platform surface. Runs conducted at a platform temperature of 20°C and relative humidity less than 70% will generally
not cause condensation. Platform temperatures greater than 20°C allow for higher relative
humidity without condensation. Environments for which condensation occurs on the platform
surface may vary. If condensation occurs, the unit may require conditioned air to reduce
temperature and relative humidity.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
4 Installation
4.1 Site requirements
Power requirements
Parameter
Specification
Mains supply voltage
100-240 V AC ±10%
Frequency
50/60 Hz
Power consumption
230 VA
Transient overvoltages
Overvoltage category II
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
39
4 Installation
4.2 Unpacking and transport
4.2
Unpacking and transport
Unpacking
Carefully unpack all items delivered and make sure that all items are present by comparing the contents with the packing list. If any part is missing, please contact your local
GE sales office.
Inspect all components. If any part appears damaged, please contact the courier immediately.
Please keep all packing material and use if damaged parts need to be returned for repair
or replacement.
•
Check the equipment for damage before starting assembly and installation.
•
Document any damage and contact your local GE.
•
Remove straps and packing material and stand equipment upright before starting
installation.
Transport
Before moving the system:
40
•
Disconnect all cables.
•
Lift the instrument by the base unit.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
4 Installation
4.3 Setup
4.3
Setup
Place the instrument
WARNING
The vents at the front, back, and bottom of Ettan IPGphor 3 must
not be obstructed. Air must be free to circulate for the cooling apparatus to function correctly. There should be a minimum of 10
cm unobstructed space behind the rear panel of the instrument.
Step
Action
1
Select a place for Ettan IPGphor 3 where it is possible to access the mains
power switch on the rear panel of the instrument.
2
Position the instrument on a flat surface with the spirit level in the center.
Turn the leveling feet as necessary to make the instrument level and stable.
Power connection
WARNING
Make sure that the supply voltage at the wall outlet corresponds
to the marking on the instrument, before connecting the power
cord.
WARNING
Only use power cords delivered or approved by GE.
WARNING
The Ettan IPGphor 3 must always be connected to a grounded
power outlet.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
41
4 Installation
4.3 Setup
WARNING
Do not block the rear and side panel of Ettan IPGphor 3. The power
switch must always be easy to access. The power cord must always
be easy to disconnect.
Two power cords are supplied: one cord has a “North American” style (UL817) 3-pronged
plug for 115 V AC power outlets, and the other has a “Central European” style (CEE7/VII)
plug for 230 V AC power outlets.
Step
Action
1
Select the cord with the appropriate plug for the power outlet in your laboratory. If neither of these cords are suitable for your power outlets, obtain
a detachable cord with an IEC/320/C13 (CEE22/V) outlet.
2
Plug the connectors into the power cord receptacle on the rear panel and
into a properly grounded power outlet.
Turn on power and run
diagnostic program
Turn on the instrument and run a diagnostic program according to the description in
Section 5.2 Start up the instrument, on page 66.
Serial port connector for a
computer connection
NOTICE
Any computer used with the equipment shall comply with IEC 60950
and be installed and used according to the manufacturer's instructions.
The RS232 serial port for a computer connection is placed at the rear of Ettan IPGphor
3. The type of cable required (shielded straight serial or shielded null modem) depends
on the type of device (DTE or DCE) that it is connected to:
42
•
If the computer is configured to receive data on pin 2 and transmit on pin 3, a regular
straight serial cable is required.
•
For any other configuration, a null modem cable is required.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
4 Installation
4.3 Setup
Pin number assignments and device set up details are mentioned below:
Ettan IPGphor 3 RS232 signal and pin
number assignments
Pin 2
Transmit
Pin 3
Receive
Pin 5
Ground
Other pins
Not connected
The Ettan IPGphor 3requires these settings in the device receiving data
Baud rate
9600
Data bits
8
Stop bit
1
Start bit
1
Parity
None
Flow control
None
Set the baud rate
Follow the instructions below to set the baud rate on the LCD screen.
Step
Action
1
Press the Up and Down arrows together to access the Baud menu.
2
Use the Up or Down arrows to set the baud rate.
3
Use the Right arrow to exit to the main menu.
S e r ia l Po r t S et u p
Baud Rate: 9600
Set Up or Dn
Exit>
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
43
4 Installation
4.3 Setup
Assembly
The following parts must be added to Ettan IPGphor 3 instrument before it can be used:
•
Strip holder or manifold
•
Strips
•
Buffer and sample liquids
Spare parts and accessories
For correct up to date information on spare parts and accessories visit:
www.gelifesciences.com
44
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
4 Installation
4.4 Software installation for isoelectric focusing process
4.4
Software installation for isoelectric focusing process
Introduction
Refer to Ettan IPGphor 3 Control Software User Manual to install the Ettan IPGphor 3
Control Software.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
45
5 Operation
5
Operation
Introduction
This chapter provides instructions for the use of Ettan IPGphor 3
Isoelectric Focusing System.
Before starting the Ettan IPGphor 3 Isoelectric Focusing System, rehydrate the samples
according to the method selected, either fixed-length Ettan IPGphor 3 strip holders or
use the Ettan IPGphor Cup Loading Manifold.
In this chapter
This chapter contains the following sections:
Section
46
See page
5.1 Preparations before start
47
5.2 Start up the instrument
66
5.3 Perform a run from the control panel
68
5.4 Run a pre-programmed protocol from the PC
90
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.1 Preparations before start
5.1
Preparations before start
Introduction
This section provides instructions on how to prepare the strip holders or the manifolds
before starting the system.
Precautions
NOTICE
Solutions containing urea may be warmed briefly. Do not heat any
solutions containing urea above 30ºC to 40ºC as isocyanate, a urea
degradation product, will carbamylate the proteins in the sample,
thus changing their isoelectric points.
NOTICE
All chemicals should be of the highest purity. Double distilled water
should be used.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
47
5 Operation
5.1 Preparations before start
5.1.1 Use the strip holders
5.1.1
Use the strip holders
Introduction
Ettan IPGphor 3 Isoelectric Focusing System fixed-length strip holders allow IPG strips
to be rehydrated and samples loaded in one step before proceeding automatically to
perform the separation. The IPG strips are 3 mm wide and 0.5 mm thick after rehydration.
Precautions
WARNING
Check that the high voltage is turned off before opening the safety
lid. The HV ON indicator on the front panel should not light.
Prepare the strip holder(s).
NOTICE
Handle the ceramic holders with care, as they are brittle and fragile.
48
Step
Action
1
Select the strip holder(s) corresponding to the IPG strip length chosen for
the experiment (7, 11, 13, 18, or 24 cm).
2
Wash each holder with the strip holder cleaning solution supplied to remove
residual protein.
3
Rinse thoroughly with double distilled water.
4
Use a cotton swab or a lint-free tissue to dry the holder or allow it to air-dry.
The holder must be completely dry before use.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.1 Preparations before start
5.1.1 Use the strip holders
Rehydrate the
Immobiline DryStrip
Follow the instructions below to rehydrate the Immobiline Drystrip.
Step
Action
1
Prepare the rehydration solution and rehydrate the Immobiline DryStrip in
the strip holder or in the rehydration tray when using the manifold.
2
Follow the recommended procedures found in the Instructions attached to
each Immobiline DryStrip package.
Note:
See also the 2-D Electrophoresis, Principles and Methods guide containing
commonly used recipes.
Pipet the rehydration solution
WARNING
Avoid spilling liquids on the body of the instrument. If large volumes
of liquid have penetrated the casing of the instrument and come
into contact with the electrical components, immediately switch
off the instrument and contact an authorized service technician.
CAUTION
Always wear gloves when handling IPG strips and the equipment
that comes in contact with them. This will help minimize protein
contamination which can result in artifactual spots in stained 2-D
gel patterns.
NOTICE
Use the appropriate rehydration volume for the IPG strip length.
Follow the instructions below to pipet the appropriate volume of rehydration solution
into each holder. The correct volume to each strip length is found in the instructions attached to each Immobiline DryStrip package.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
49
5 Operation
5.1 Preparations before start
5.1.1 Use the strip holders
50
Step
Action
1
Pipette the solution slowly at a central point in the strip holder channel away
from the sample application wells.
2
Remove any larger air bubbles.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.1 Preparations before start
5.1.1 Use the strip holders
Position the IPG strip
Follow the instructions below to postion the IPG strip.
Step
Action
1
Remove the protective cover from the IPG strip.
2
Position it with the gel side down and the pointed (anodic) end of the strip
directed toward the pointed end of the strip holder.
3
With the pointed end first, lower the strip onto the solution. To help coat the
entire strip, gently lift and lower the strip and slide it back and forth along
the surface of the solution, tilting the strip holder slightly as needed to assure
complete and even wetting.
4
Lower the cathodic (square) end of the strip into the channel, making sure
that the IPG gel is in contact with the strip holder electrodes at each end.
The gel can be visually identified once the rehydration solution begins to
dye the gel. Be careful not to trap bubbles under the strip.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
51
5 Operation
5.1 Preparations before start
5.1.1 Use the strip holders
Apply Immobiline DryStrip
cover fluid
Follow the instructions below to apply Immobiline DryStrip Cover Fluid to minimize
evaporation and urea crystallization.
Step
Action
1
Pipet the cover fluid dropwise into one end of the strip holder until one half
of the strip is covered.
2
Later, pipet the cover fluid dropwise into the other end of the strip holder,
adding fluid until the entire IPG strip is covered.
Place the strip holder cover
Position the cover on the holder. Pressure blocks on the underside of the cover ensure
that the strip maintains good contact with the electrodes as the gel rehydrates.
Rehydration proceeding
Rehydration can proceed on the bench top or on the Ettan IPGphor 3 platform. Ensure
that the holder is on a level surface. A minimum of 10 h is required for rehydration,
overnight is recommended.
Alternatively, the rehydration period can be programmed as the first step of an
Ettan IPGphor 3 protocol. This is especially convenient if temperature control during rehydration is a concern, or if a low voltage is applied during rehydration.
Sample rehydration under
voltage (optional)
Follow the instructions below to perform rehydration under voltage. If desired, the
sample can be absorbed by the IPG strip under low voltage. This may improve the uptake
of high molecular weight proteins.
52
Step
Action
1
Set the rehydration time to 0:00 and program Step 1 of the protocol for low
voltage (30 to 100 V) for 10 to12 h, see Edit step or gradient, on page 79
2
Program additional steps as desired to achieve complete focusing.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.1 Preparations before start
5.1.1 Use the strip holders
Apply electrode pads prior to IEF
(optional)
Under certain conditions, such as prolonged focusing, water may migrate toward one
end of the strip, causing the other end to begin drying out. This effect can be minimized
by placing paper electrode pads between the IPG strip and each strip holder electrode
just before IEF. Electrode pads may also absorb ions that would otherwise accumulate
at the ends of the IPG strip and possibly interfere with the separation.
Follow the instructions below to apply electrode pads prior to IEF.
Step
Action
1
Cut two 3 mm wide electrode pads from a paper IEF electrode strip (18-100440).
2
Position on a clean, flat surface such as a glass plate and soak with deionized
water. Remove excess water by blotting with tissue paper.
Note:
Electrode pads must be damp, not saturated or dripping.
3
Lift one end of the rehydrated IPG strip using forceps or tweezers. Position
an electrode pad over the electrode, then lower the strip back in place.
4
Repeat at the other end.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
53
5 Operation
5.1 Preparations before start
5.1.1 Use the strip holders
Apply sample after rehydration
(optional)
If the sample was not applied by inclusion in the rehydration solution, it can be applied
immediately prior to IEF.
Follow the instructions below to apply electrode pads prior to IEF.
Note:
The IPG strip backing is impermeable; do not apply the sample to the back of
the strip.
Step
Action
1
Prepare the sample in a solution similar in composition to the rehydration
solution used.
2
Pipet the sample into either or both of the lateral wells at either end of the
strip holder. Introduce the sample below the cover fluid.
Up to 7.5 μl of sample solution can be added to each side (i.e. 15 μl per well
or maximum 30 μl if both sides of both wells are used).
54
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.1 Preparations before start
5.1.1 Use the strip holders
Position the strip holders
Ettan IPGphor 3 platform has two electrode areas:
•
The larger area (3) is the positive electrode (anode)
•
The smaller area (4) is the negative electrode (cathode).
Position the strip holder on the platform as shown below: Electrode contacts underneath,
one in each electrode area (1). The pointed end of the strip holder (2) is over the anode
(pointing to the back of the unit) and the blunt end is over the cathode. Guidemarks along
the sides of the platform show approximate positioning for each strip holder size (7, 11,
13, 18, and 24 cm).
Electrode contacts underneath,
1 in each electrode area
one
The
2 pointed end of the
strip holder is in the anodic
electrodae area.
Anodic
3 electrode area (+)
Cathodic
electrode area (–)
4
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
55
5 Operation
5.1 Preparations before start
5.1.1 Use the strip holders
Using safety lid
NOTICE
During isoelectric focusing, do not lean on the safety lid, do not
apply excess pressure or uneven weight to the lid, and do not place
any items on the lid. Such pressure could cause arcing between
the strip holder electrodes and the electrode areas, damaging the
instrument.
When using the Ettan IPGphor standard strip holders, lid adapters must be used to apply
the correct amount of pressure to the lids of the IPGphor strip holders. The pressure
keeps the IPG strip in contact with the IPG strip holder electrodes.
56
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.1 Preparations before start
5.1.1 Use the strip holders
Close the safety lid
CAUTION
The safety lid must be properly closed before power is applied.
NOTICE
Exceeding the recommended current limit of 50 µA per IPG strip
can cause the strip to burn and may damage the instrument.
Follow the instructions below to ensure good electrical contact when using the fixed
length strip holder:
Step
Action
1
Apply two lid adapters (2) right across the lids of the strip holders, one over
the anodic area and the other over the cathodic area.
1
2
3
2
Use two or more strip holders (3) and place them wide apart to ensure that
the lid adapters are only hanging on one strip holder and not pressing down
on the other.
3
Carefully close the safety lid (1) by applying light downwards pressure, ensure
the mechanism locks the lid.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
57
5 Operation
5.1 Preparations before start
5.1.1 Use the strip holders
Step
Action
4
To inspect the run the light protective cover can be lifted without stopping
the run.
Light prot
cover
5
58
To open the safety lid press down the lid and the lock is released and the
run is stopped. Close the lid and the run continues.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.1 Preparations before start
5.1.2 Use the Ettan IPGphor Cup Loading Manifold
5.1.2
Use the Ettan IPGphor Cup Loading Manifold
Precautions
CAUTION
Always wear protective gloves when working with rehydration solution.
Introduction
For more detailed information, please refer to Ettan IPGphor Cup Loading Manifold User
Manual.
Rehydrate Immobiline DryStrip
in the IPGbox
Rehydrate the IPG strips with the gel side down in the appropriate volume of rehydration
solution, with or without sample, using the IPGbox.
Rehydration in the Cup Loading Manifold is not recommended, the channel is too wide
to ensure proper rehydration.
Also, follow the instructions included with the Immobiline DryStrips or the IPGbox. Close
the lid of the IPGbox and allow the strips to rehydrate overnight (10 to 20 hours).
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
59
5 Operation
5.1 Preparations before start
5.1.2 Use the Ettan IPGphor Cup Loading Manifold
Position the manifold on
Ettan IPGphor 3
Follow the instructions below to position the manifold on Ettan IPGphor 3.
60
Step
Action
1
Clean and dry the Ettan IPGphor 3 platform before placing the manifold on
the system.
2
Position the manifold on the Ettan IPGphor 3 platform. The small T-shaped
protrusion fits into a cutout section of the Ettan IPGphor 3 platform near the
lid hinge.
3
Ensure that the manifold is level by placing the round spirit level on the
center of the manifold tray after it is placed on the Ettan IPGphor 3.
4
Measure out 108 ml of Immobiline DryStrip Cover Fluid (even if fewer than
12 strips will be loaded into the manifold). Add the cover fluid evenly in the
12 manifold channels.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.1 Preparations before start
5.1.2 Use the Ettan IPGphor Cup Loading Manifold
Position the IPG strips in the
manifold
Follow the instructions below to position the IPG strips in the manifold.
Step
Action
1
Transfer the strips to the Ettan IPGphor Cup Loading Manifold.
2
Position the strips under the cover fluid face up in the tray with the anodic
(+, pointed) end of the IPG strip pointing at the anode of Ettan IPGphor 3.
3
Center the strip down the length of the manifold channel. Protrusions along
the sides guide the strip approximately straight, although some manual
adjustment of the strip may be necessary. See also the Ettan IPGphor Cup
Loading Manifold User Manual.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
61
5 Operation
5.1 Preparations before start
5.1.2 Use the Ettan IPGphor Cup Loading Manifold
Place sample cups
Follow the instructions below to place the sample cups.
Step
Action
1
Position a strip of sample cups in the appropriate position, for example ~1
cm from the end of the gel portion of the IPG strip. Do NOT place the cup
with the feet over a center protrusion.
2
Ensure that the feet of the cups are properly seated at the bottom of the
channel.
Use the insertion tool, wiggle the tool gently while pushing it down.
62
3
Fill the cups with cover fluid to test for proper seating of the cups.
4
Replace the cover fluid after 10 minutes.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.1 Preparations before start
5.1.2 Use the Ettan IPGphor Cup Loading Manifold
Place electrode paper wicks
Follow the instructions below to place the electrode paper wicks.
Step
Action
1
Add 150 μl distilled water to each paper wick.
2
Position the wicks on each end of the IPG strips so that one end of the wick
overlaps the end of the gel on the IPG strip.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
63
5 Operation
5.1 Preparations before start
5.1.2 Use the Ettan IPGphor Cup Loading Manifold
Position the electrodes
Follow the instructions below to position the electrodes.
64
Step
Action
1
With the electrode cams in the open position, position the electrode assembly
on top of all the wicks. The electrode must be in contact with the wick.
2
Swivel the cams into the closed position under the external lip of the tray.
The electrodes should not be moved while the cams are in the closed position.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.1 Preparations before start
5.1.2 Use the Ettan IPGphor Cup Loading Manifold
Load samples into the sample
cups
Follow the instructions below to load the samples into the sample cups.
Step
Action
1
A maximum of 150 μl of sample may be placed in these cups.
2
Check to make sure that there is cover fluid over the samples. When the
sample is introduced into the cups, it will sink through the oil to the bottom
of the cup and come into contact with the IPG strip.
3
Close the Ettan IPGphor 3 lid.
4
•
Program and run the Ettan IPGphor 3 from its own control panel display.
See Section 5.3 Perform a run from the control panel, on page 68 for more
details, or
•
Start the Ettan IPGphor 3 from the Ettan IPGphor 3 Control Software.
See Section 5.4.1 Setting up a run, on page 91 for more details.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
65
5 Operation
5.2 Start up the instrument
5.2
Start up the instrument
Precautions
WARNING
Check that the high voltage is turned off before opening the safety
lid. The HV ON indicator on the front panel should not light.
WARNING
In a situation where there is a risk of injury, turn off the instrument
by switching the mains power switch (located on the rear panel)
to position 0. Malfunctions must be rectified before Ettan IPGphor 3
Isoelectric Focusing System is restarted.
Turn on power and run
diagnostic program
Step
Action
1
Turn on the mains Power switch located on the rear panel.
2
This activates a self diagnostic program that runs for approximately 10
seconds. A series of screens indicate progress. If any component fails, the
diagnostic program will stop and a message will indicate the source of the
fault.
Note the fault, if any.
66
3
Press START to skip to the next test.
4
Call GE service if any failure is detected,
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.2 Start up the instrument
Step
Action
5
Once the diagnostic program is successfully completed, the LCD screen indicates that the instrument is ready, as shown in the figure.
Pr o t # 1 F i l e 1
Rehydrate 0:00H r @ 20 ˚ C
IEF @ 20˚C 50µA/Strip
1 Strips 0 Steps
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
67
5 Operation
5.3 Perform a run from the control panel
5.3
Perform a run from the control panel
In this section
This section covers how you run ISO electric focusing from the control panel.
Section
68
See page
5.3.1 Ettan IPGphor 3 control panel functionality
69
5.3.2 Protocol parameters
73
5.3.3 Set protocol parameters
75
5.3.4 Isoelectric focusing from the control panel
83
5.3.5 Pause or stop a protocol
89
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.3 Perform a run from the control panel
5.3.1 Ettan IPGphor 3 control panel functionality
5.3.1
Ettan IPGphor 3 control panel functionality
Introduction
IEF runs are programmed from theEttan IPGphor 3 Isoelectric Focusing System control
panel with up to ten user-defined IEF protocols, each with up to 9 steps per protocol.
Programmable functions including: rehydration time, platform temperature, current
limit, voltage limit for each step, voltage gradient or step and step duration.
Control unit keys
The following keys on the control panel are used to program Ettan IPGphor 3.
COMMAND
keys
Function
EDIT
Pressing the EDIT key in the edit mode toggles between the prorocol identification screen with general settings for all steps and the
settings in the three first protocol steps.
The EDIT key switches the display to edit mode, in which protocol
steps can be programmed. (Refer to Section 5.3.3 Set protocol parameters, on page 75.
The EDIT key also allows the estimated run time remaining to be
viewed while the unit is operating.
Left arrow
The Left arrow key moves the cursor to the left. Moving the cursor
off the screen to the left repositions the cursor at the left side of
the next row. From the bottom row the cursor is repositioned on
the left side of the top row.
Right arrow
The Right arrow key moves the cursor to the right. Moving the
cursor off the screen to the right repositions the cursor at the left
of the next row. From the bottom row the cursor is repositioned
on the left side of the top row.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
69
5 Operation
5.3 Perform a run from the control panel
5.3.1 Ettan IPGphor 3 control panel functionality
COMMAND
keys
Function
Up arrow
The Up arrow key increases the value of the selected field or digit.
Note:
Exception is when the cursor is positioned to the left under the Step
number S1-S9, the Up arrow key changes the display to the previous
group of three protocol steps.
Note:
During electrophoresis, the Up/dDown arrow keys are used to
toggle between VhS, VhT, and Hrs.
Down arrow
The Down arrow arrow key decreases the value of the selected
field or digit.
Note:
Exception is when the cursor is positioned to the left under the Step
number S1-S9, the Down arrow key changes the display to the next
group of three protocol steps..
Note:
During electrophoresis, the Up/Down arrow keys are used to toggle
between VhS, VhT, and Hrs.
START
The START button begins the selected protocol.
STOP
The STOP button pauses the protocol. Pressing the STOP key a
second time cancels the protocol in progress.
Press the STOP button a third time to reset the instrument to start
another protocol.
HV ON indicator lamp
70
The HV ON lamp indicates that the high voltage power supply is
on and isoelectric focusing is in progress. This lamp is not illuminated during the rehydration phase of a protocol.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.3 Perform a run from the control panel
5.3.1 Ettan IPGphor 3 control panel functionality
Protocol identification screen
The protocol identification screen is the first screen that appears after the diagnostic
program is completed. The frist row starts with Protocol #1, Name. The default location
of the cursor is always under the protocol number “1".
A protocol can be selected by pressing the Up and Down arrow keys to display the
number of a protocol. Once the selected protocol is started, the LCD screen indicates
the parameters of the step currently being carried out.
The protocol identification screen comprises the following programmable items:
•
Protocol number and name
•
Rehydration time, temperature
•
IEF run conditions (temperature and maximum current/strip)
•
Number of strips and number of steps in the selected protocol.
Pr o t# 1 F i l e 1
Rehydrate 0:00H r @ 20 ˚ C
IEF @ 20˚C 50µA/Strip
1 Strips 0 Steps
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
71
5 Operation
5.3 Perform a run from the control panel
5.3.1 Ettan IPGphor 3 control panel functionality
IEF steps screen
The second screen in edit mode is the first in a series of nine programmable IEF steps
in which the voltage change pattern, voltage, and step duration are set. The steps are
displayed in groups of 3 steps per screen. The number of steps which have been programmed is displayed next to the protocol number.
The two options for the voltage change pattern are:
•
Step, which sets the voltage at the selected value for the new step and then holds
the voltage constant for the step duration.
•
Gradient, which increases the voltage limit (linearly with respect to time) from the
value set for the previous step to the value set for this step. If this is the first step,
the value increases from 0.
The original factory programmed voltage for each step is 0. Program each step with the
protocol voltage values. If fewer than nine steps are required, end the program by assigning a value of 0 V after the final step. The step duration (0 to 99:59 hours) or volthours (0 to 300.000 Vhr) are programmable for each step.
Pr o t# 1
S1 Step
S2 Step
S3 Step
72
3 Steps
500V 1:00 H rs
1000V 4:00 H rs
8000V 80000 Vhr
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.3 Perform a run from the control panel
5.3.2 Protocol parameters
5.3.2
Protocol parameters
Programmable parameters
Ettan IPGphor 3 can store up to ten protocols. Programmable protocol parameters include:
•
Duration and temperature of the rehydration phase.
•
Duration, temperature, and power parameters for up to nine IEF steps for each protocol.
•
Power parameters including maximum current, voltage, and voltage change pattern.
Please refer to the 2-D Electrophoresis, principles and methods guide for sample handling
recommendations and protocol guidelines. The permitted range of values for each programmable parameter is listed below.
Permitted range of values for each programmable parameter
Parameter
Operational Range
Steps
1 to 9
Step duration
Rehydration: 0 to 99:59 hrs
10:00 to14:00 hrs
Focusing: 0 to 99:59 hrs
2:00 to 10:00 hrs
Volts
0 to 10000 V
100 to 10000 V
Max. current
1500 μA total for 12 strips max.
50–75 μA per strip
Platform temperature
Rehydration: 15°C to 30°C
20
Focusing: 15°C to30°C
20
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
Recommended values
73
5 Operation
5.3 Perform a run from the control panel
5.3.2 Protocol parameters
Navigating parameter “fields”
and “digits”
•
Each screen consists of information as well as one or more fields that can be edited.
The active fields contain individually adjustable digits or characters.
•
Certain digits are also linked to their neighbouring digits. For example, three of the
four digits in the voltage field can each be adjusted individually, but when adjusting
the value from 1000 to 990, for instance, even though only the ten’s place is adjusted
manually, three digits are affected.
•
Whether individual or linked, digits “wrap around,” so increasing from 9 produces 0
and decreasing from 0 produces 9—until a parameter limit is reached.
Ettan IPGphor 3 can be programmed in volt-hours (Vhr) or hours and minutes in step-nhold or in gradient mode. Any values within the operating limits listed above can be used.
74
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.3 Perform a run from the control panel
5.3.3 Set protocol parameters
5.3.3
Set protocol parameters
Introduction
This section covers the programming, editing, or verifying of all parameter values.
All protocol parameters are set in the edit mode. Press the EDIT key to toggle between
the first editable screen and the protocol identification screen.
Note:
The cursor stops only in the fields that can be edited. Once the cursor is in position, press the Up or Down arrow keys to adjust the value.
Create or edit protocols
Follow the instructions below to edit the rehydration parameters, time and temperature,
and the IEF parameters, temperature, μA/Strip and number of strips as necessary on
the main screen.
Step
Action
1
Select the protocol to be created or edited from the protocol identification
screen.
2
Position the cursor under the protocol number field using the Right and Left
arrow keys and move to the desired protocol number using the Up and
Down arrow keys.
Pr o t# 1 F i l e 1
Rehydrate 10:00H r @ 20 ˚ C
IEF @ 20˚C 50µA/Strip
1 Strips 0 Steps
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
75
5 Operation
5.3 Perform a run from the control panel
5.3.3 Set protocol parameters
Step
Action
3
Edit the file name by using the Right arrow key to move into the file name,
and Up and Down arrows to adjust the characters as desired. To skip editing
the name use the Left arrow to proceed to the next line.
To change any letter, move the cursor to that location using the Right and
Left arrow keys and then scroll through the 37 available options using the
Up and Down arrow keys. Once the desired letter appears, move the cursor
to the next character to be set.
The original label “File #” occupies 6 of the 16 available characters. To change
the label to a more descriptive name, such as “11 CM 3 to 10L,” requires
scrolling through the alphanumeric options for each of the 11 digits in the
name.
Pr o t# 1 F i l e 1
Rehydrate 10:00H r @ 20 ˚ C
IEF @ 20˚C 50µA/Strip
1 Strips 0 Steps
4
Edit time range by using the Left and Right arrow keys to position the cursor,
and use the Up and Down arrow keys to set the time range.
The available time range is 0–99:59 h, with 10 min resolution. (Hence, only
the first three digits are editable). A minimum of 10 hrs is required for the
rehydration of IPG strip gels, but overnight is recommended. This protocol
step can also be used as a simple timer to start the protocol at a convenient
time. If the IPG strips are already rehydrated and ready for immediate focusing, set the rehydration duration to 00:00 Hrs.
Pr o t# 1 F i l e 1
Rehydrate 10:00H r @ 20 ˚ C
IEF @ 20˚C 50µA/Strip
1 Strips 0 Steps
76
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.3 Perform a run from the control panel
5.3.3 Set protocol parameters
Step
Action
5
Adjust the rehydration temperature by using the Left and Right arrow keys
to position the cursor. Use the Up and Down arrow keys to select the value
within the range of 15°C to 30°C.
The original factory programmed value is 20°C.
Pr o t# 1 F i l e 1
Rehydrate 10:00H r @ 20 ˚ C
IEF @ 20˚C 50µA/Strip
1 Strips 0 Steps
Note:
Maintaining the actual platform temperature at <18 °C or >25 °C may require
placement of the Ettan IPGphor 3 instrument in a location of lower or higher
than ambient temperature (e.g. a cold room).
It may be necessary to operate the instrument in an air conditioned room to
prevent condensation from collecting on the platform in humid environments
when the platform is run below 7°C ambient temperature.
6
The third line contains fields to set the platform temperature and current
limit for all isoelectric focusing steps.
Adjust the rehydration temperature by using the Left and Right arrow keys
to position the cursor. Use the Up and Down arrow keys to select the value
within the range of 15°C to 30°C.
To set the platform temperature use the Left and Right arrow keys to position the cursor in the active °C field and use the Up and Down arrow keys
to select the value within the range of .
The original factory programmed IEF temperature is 20°C.
Pr o t# 1 F i l e 1
Rehydrate 10:00H r @ 20 ˚ C
IEF @ 20˚C 50µA/Strip
1 Strips 0 Steps
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
77
5 Operation
5.3 Perform a run from the control panel
5.3.3 Set protocol parameters
Step
Action
7
Adjust the current limit for all isoelectric focusing steps by using the Left
and Right arrow keys to position the cursor under one of the three active
μA digits, and use the Up and Down arrow keys to select the value within
the range of 0 to 200 μA per strip.
The original factory programmed value is 50 μA per IPG strip.
Pr o t# 1 F i l e 1
Rehydrate 10:00H r @ 20 ˚ C
IEF @ 20˚C 50µA/Strip
1 Strips 0 Steps
Note:
The total deliverable ampeerage is 1500 μA.
8
Use the arrow keys to adjust the numbers of strips to run.
Pr o t# 1 F i l e 1
Rehydrate 10:00H r @ 20 ˚ C
IEF @ 20˚C 50µA/Strip
1 Strips 0 Steps
9
78
Press EDIT on the control panel to move to next screen.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.3 Perform a run from the control panel
5.3.3 Set protocol parameters
Edit step or gradient
1
Press the EDIT key to display the programmable parameters on the display.
Position the cursor in the field to be edited using the Right and Left arrow
keys and adjust the value by pressing the Up and Down arrow keys.
2
Access a Step by placing the cursor under the step number and use the
Right and Left arrow keys.
Each step is identified by “S” and the step number. The original programmed
option is Step for each step.
Pr o t# 1
S1 Step
S2 Step
S3 Step
3 Steps
500V 1:00 H rs
1000V 4:00 H rs
8000V 80000 Vhr
3
To advance to the next step group press the Down arrow key and to return
to a previous step group press the Up arrow key.
4
To toggle to Gradient, move the cursor to the Step field using the Right and
Left arrow keys and toggle with the Up or Down arrow key.
Pr o t# 1
S1 Grad
S2 Grad
S3 Step
5
3 Steps
500V 1:00 Hrs
1000V 4:00 Hrs
8000V 80000 Vhr
IEF voltage is programmable from 0–8000 V in increments of 10 V. Each of
the first three digits in the voltage field can be set independently.
First move the cursor to the desired digit location using the Right and Left
arrow keys and then change the value using the Up and Down arrow keys.
Pr o t# 1
S1 Grad
S2 Grad
S3 Step
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
3 Steps
500V 1:00 Hrs
2000V 1:00 Hrs
8000V 2:00 Hrs
79
5 Operation
5.3 Perform a run from the control panel
5.3.3 Set protocol parameters
6
To select, set the cursor under the currently selected field and use the Up
or Down arrow key to toggle between Hrs and Vhrs. Move the cursor to the
desired digit location using the Right and Left arrow keys, and change the
value using the Up and Down arrow keys.
The original factory programmed time for each step is 0:00, and should be
set to 0:00 for all unused steps. The Vhrs value is calculated according to
the explanatory note at the left, but these values can be set in the same
manner as the Hrs values.
Note:
When toggling from Hrs to Vhr, the value in the Vhrs field is automatically
calculated according to the selected voltage change pattern:
For Step-n-hold, Vhr is the product of hours and voltage.
For Gradient, Vhr is the product of hours and the mean of the voltage limit
set for the current step and the previous step.
Absolute Vhr limits can be set to any value from 1 to 30000 Vhr.
Pr o t# 1
S1 Grad
S2 Grad
S3 Step
80
3 Steps
500V 1:00 Hrs
1000V 4:00 Hrs
8000V 80000 Vhr
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.3 Perform a run from the control panel
5.3.3 Set protocol parameters
Simplified 4-step protocol and
resulting voltage profile
(example)
To illustrate the two voltage change patterns, a simplified example shows program steps
and the resulting voltage profile. Note that step 4 ends the protocol.
For recommended IEF protocols, see the 2-D Electrophoresis Principles and Methods
guide.
Note:
The set voltage is the voltage limit. Under conditions of limiting current, it may
take some time for the maximum voltage to be reached, and under certain
power conditions, it may not be reached at all.
A lower voltage step may be entered at the end of the Ettan IPGphor 3 protocol. Band
sharpness will decrease however.
Verify the protocol
Step
Action
1
Scroll through each step: position the cursor in the step number field using
the Right and Left arrow keys.
2
Advance through the steps using the Up and Down arrow keys.
3
Edit as required by placing the cursor with the Right and Left arrow keys
and adjusting the value with the Up and Down arrow keys.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
81
5 Operation
5.3 Perform a run from the control panel
5.3.3 Set protocol parameters
Step
Action
4
Make sure that the voltage field in all unused steps displays a value of 0, or
that the value in the Hrs field is 00:00.
Exit edit mode
Press EDIT to save any changes and exit the step edit mode. The protocol identification
screen will appear.
82
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.3 Perform a run from the control panel
5.3.4 Isoelectric focusing from the control panel
5.3.4
Isoelectric focusing from the control panel
Precautions
WARNING
Ettan IPGphor 3 is a high voltage instrument that can cause fatal
electrical shock if the safety features are disabled. The safety lid
must be securely closed before starting a protocol.
Protocol steps
Once the protocol begins, each step is carried out automatically, and instrument status
for each step is indicated on the screen. Platform temperature and time remaining can
be changed while the protocol is in progress, and each such possibility is discussed in
turn below.
Select protocol number
Step
Action
1
Place the cursor in the protocol number field using the Right and Left arrow
keys and select the protocol number using the Up and Down arrow keys.
Pr o t # 1 M Y P R O TO C O L
Rehydrate 10:00H r @ 20 ˚ C
IEF @ 20˚C 200µA/Strip
12 Strips 3 Steps
2
Use the Right and Left arrow keys to position the cursor to set the number
of strips to run, and the up and Down arrow keys to select the number(1–12)
Pr o t # 1 M Y P R O TO C O L
Rehydrate 10:00H r @ 20 ˚ C
IEF @ 20˚C 200µA/Strip
12 Strips 3 Steps
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
83
5 Operation
5.3 Perform a run from the control panel
5.3.4 Isoelectric focusing from the control panel
Step
Action
3
Press START. A screen is displayed to confirm the number of strips to run.
Adjust the number of strips if necessary.
Number of strips :12
P r e s s S TA R T t o c o n t i n u e
4
Press START to continue.
5
If the safety lid is not properly closed the following message will indicate the
required action:
Lid open step 1
Close lid to continue
CAUTION
During IEF:
6
84
•
Do not lean on the safety lid.
•
Do not apply excess pressure or uneven weight to
the lid.
•
Do not place any items on the lid.
Press START again after securing the safety lid.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.3 Perform a run from the control panel
5.3.4 Isoelectric focusing from the control panel
Step
Action
7
The first screen will indicate the protocol, number of steps, and total hours
(excluding rehydration time):
Starting protocol # 1
3 steps 4:00 Hr
If a rehydration period was programmed, the rehydration status screen will
indicate the number of hours elapsed and the actual platform temperature:
Runni n g Pr o t# 1
Rehydration for 10:00 H r
0:01 Hr Elapsed
Rehydrate at 2 0 ˚ C
Change rehydration duration or
other parameters
To change rehydration duration or other parameters:
Step
Action
1
Press EDIT to display the set screen. Pressing EDIT again will access the step
setting screen. Pressing EDIT again will return to the running screen.
2
The first line indicates that the protocol is running.
Any changes made while a protocol is running are applied to the current
run only, the stored protocol is not affected. Move the cursor under the digit
you wish to set and change it using the Up and Down arrow keys. To end
this step, set all digits to 0. This screen is automatically replaced by the regular status screen after a few seconds.
Runni n g Pr o t# 1
Rehydrate 10:00H r @ 20 ˚ C
IEF @ 20˚C 200 µA/Strip
12 Strips 3 Steps
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
85
5 Operation
5.3 Perform a run from the control panel
5.3.4 Isoelectric focusing from the control panel
Step
Action
3
The temperature field can be changed directly (without pressing EDIT)
Use the Right and Left arrow keys to position the cursor in the temperature
field and use the Up and Down arrow keys to adjust the value.
It may take up to 30 minutes to reach the set temperature, depending on
ambient conditions.
Isoelectric focusing will begin after the rehydration step is complete. The
HV ON lamp will light and the status screen showing the step currently in
progress as well as all parameters will appear.
Runni n g Pr o t# 1 @ 2 0 ˚ C
Step 1 of 3 Vhrs
Step Vhrs Elapsed
500V 40µA/Strip 0.0W
Note:
The indicated wattage is the total power delivered to all strips, and will often
read 0 W because any value less than 0.5 is rounded down to 0. If the delivered
power exceeds 0.4 W per strip, stop the run and reduce the V or μA settings.
The current platform temperature is indicated.
The average current per IPG strip (μA), the total instantaneous power delivered (W), and actual voltage (V) are indicated. If the current is the limiting
parameter, the “A” in “μA” will flash.
4
To toggle between VhT, VhS and Hrs:
•
Move the cursor to the Hrs/Vhrs field of the display using the Right or
Left arrow key.
•
Change the display to reflect VhT, VhS and Hrs using the up and down
keys.
Runni n g Pr o t# 1 @ 2 0 ˚ C
Step 1 of 3 Vhrs
Step Vhrs Elapsed
500V 40µA/Strip 0.0W
The Ettan IPGphor 3 will display the volt-hours during the run as:
•
VhS, which indicates the total volt hours for that step, or
•
VhT, the total volt-hours accumulated during the entire run at the current
time.
Hours elapsed for the current step will be indicated by Hrs.
86
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.3 Perform a run from the control panel
5.3.4 Isoelectric focusing from the control panel
Step
Action
5
To change the display to show remaining Hrs, VhS or VhT:
Move the cursor under the Elapsed or ~ Remain field and use the Up or
Down arrow keys.
The temperature, voltage and current limits for the current step may be
adjusted directly on this screen as well.
Runni n g Pr o t# 1 @ 2 0 ˚ C
Step 1 of 3 Vhrs
Step Vhrs Elapsed
500V 40µA/Strip 0.0W
6
Pressing EDIT will access the step parameter screen. Pressing EDIT again
will return to the running screen. Use the Up arrow in the S1 to S3 fields to
access the Rehydration/IEF screen.
Running
S1 Grad
S2 Grad
S3 Step
Pr o t#
500V 1:00 Hrs
2000V 1:00 Hrs
8000V 2:00 Hrs
Note:
If you adjust the voltage during a gradient step the step will be converted to
Step for the remainder of the step.
7
As isoelectric focusing proceeds, the bromophenol blue tracking dye will
migrate toward the anode and leave the IPG strip. This generally occurs well
before focusing is complete
Note:
A colorless strip is no indication that IEF is complete.
If the tracking dye does not move, then no current is flowing across the strip.
Check that the strip holder electrodes are in contact with the electrode areas.
See the Troubleshooting section for additional suggestions.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
87
5 Operation
5.3 Perform a run from the control panel
5.3.4 Isoelectric focusing from the control panel
Step
Action
8
When the protocol ends, audible beeps will sound and the following screen
is displayed:
Run ended at
8 0 0 0 V 1 2 0 0 0 V h r s To t a l
5 0 µ A 8 : 0 0 H r To t a l
Pr e s s St o p t o r e s e t
The final instrument values are displayed. Press STOP, as indicated, to reset
the instrument.
88
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.3 Perform a run from the control panel
5.3.5 Pause or stop a protocol
5.3.5
Pause or stop a protocol
Start and stop keys
To pause or stop a protocol in progress, press the STOP key:
Pau s ed in s tep 1
P r e s s S TA R T t o c o n t i n u e
To continue the protocol, press the START key. To cancel the protocol, press the STOP
key again. This will cause the instrument to respond as though the end of an uninterrupted
protocol had been reached as described above.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
89
5 Operation
5.4 Run a pre-programmed protocol from the PC
5.4
Run a pre-programmed protocol from the PC
In this section
This section covers how you run ISO electric focusing from a PC.
Section
90
See page
5.4.1 Setting up a run
91
5.4.2 Perform a run
97
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.4 Run a pre-programmed protocol from the PC
5.4.1 Setting up a run
5.4.1
Setting up a run
Precautions
WARNING
Check that the high voltage is turned off before opening the safety
lid. The HV ON indicator on the front panel should not light.
Main screen and buttons
1
2
3
45
Part
Function
1
Instrument selection buttons
2
Protocol mode buttons
3
Instrument control buttons
4
Info button
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
91
5 Operation
5.4 Run a pre-programmed protocol from the PC
5.4.1 Setting up a run
Part
Function
5
Protocol view button
Connect instrument
Step
Action
1
Start the computer, log on to Windows and start Ettan IPGphor 3 Control
Software.
2
Select the desired Ettan IPGphor 3 by clicking one of the four instrument
selection buttons (Instr1 - Instr4).
The identity of the instrument should now be shown below the buttons. Up
to four instruments can be run simultaneously with the software.
3
Select menu Communication/Instrument (1-4) to open the instrument
communication settings.
4
Check Active.
5
Select COM port in the COM port list box.
6
Enter instrument name in the Name field.
7
Click Done.
A green lamp is lit in the corresponding instrument selection button.
92
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.4 Run a pre-programmed protocol from the PC
5.4.1 Setting up a run
Select protocol mode options
For setting the protocol for the run there are two main options:
•
Fast mode
•
Advanced mode
The selection is made by the protocol mode buttons and the selected mode is indicated
below the buttons.
In the fast mode option the software selects an optimized protocol based on the tray
type, strip length and pH range to be used. Further, by switching to advanced mode, this
protocol can be edited and saved to a file.
Use the fast mode protocol
Step
Action
1
In Ettan IPGphor main window, click on the appropriate instrument selection
button (Instr1 - Instr4) to select the desired instrument.
2
Select Fast. Fast should be shown below the buttons.
3
Select the Run settings & details tab by clicking the tab.
4
Set experimental conditions, that is Strip length, pH range and Number of
strips, by clicking the corresponding button.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
93
5 Operation
5.4 Run a pre-programmed protocol from the PC
5.4.1 Setting up a run
Step
Action
5
The software now selects an optimized GE protocol for the specified experimental parameters.
Edit the selected protocol
Select the Protocol view in the Run settings & details tab by clicking the right of the two
buttons at the bottom right hand corner of the tab window.
The protocol selected by the software is now shown in detail. It is now possible to edit
any values in the protocol by clicking an editable field and enter desired changes.
94
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.4 Run a pre-programmed protocol from the PC
5.4.1 Setting up a run
Save protocol
Step
Action
1
Check that the desired protocol name is entered in the Protocol name box.
Tip:
Name the protocol according to user name_strip length_pH range (upper
limit 16 characters).
2
Click the middle of the three buttons in order to save the protocol. A Save
as dialog is opened. Change protocol file name and protocol directory folder,
if desired.
Note:
If the name of an edited protocol will be overwritten it will be lost.
Download protocols to a
specific position
Ettan IPGphor 3 can store up to ten protocols.
Step
Action
1
To download a protocol to the Ettan IPGphor 3 at another position than position 1, select menu Protocol:Transfer protocol. and select desired Instrument position (1-10).
2
Click OK.
The current protocol is now downloaded to the selected position in
Ettan IPGphor 3. Any existing protocol at that position is overwritten.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
95
5 Operation
5.4 Run a pre-programmed protocol from the PC
5.4.1 Setting up a run
Delete protocols
Click on the right button of the three buttons in the top of the Protocol view.
The current protocol is now deleted.
96
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.4 Run a pre-programmed protocol from the PC
5.4.2 Perform a run
5.4.2
Perform a run
Start a run
Follow the instructions below to start the run after transferring the protocol.
Step
Action
1
Select instrument by clicking the desired Instrument selection button (Instr1Instr4).
2
Select a protocol to run.
3
Select the number of strips to run.
4
Click Start button.
The current protocol is now downloaded to position 1 in the selected instrument.
The run is now started which is indicated by the word Running below the
instrument control buttons.
5
To start a second run, select the desired instrument, protocol, and number
of strips to run, and click Start button. It is possible to start up to four different
runs using four different protocols on four different instruments in parallel.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
97
5 Operation
5.4 Run a pre-programmed protocol from the PC
5.4.2 Perform a run
Pause a run
Follow the instructions below to pause a run.
Step
Action
1
Click Pause button to pause an ongoing run.
The run is now paused which is indicted by the word Paused below the instrument control buttons.
2
Click Start button to proceed the run. The run is started from the same position in the protocol from where it was paused.
Stop a run
Follow the instructions below to stop an ongoing run.
Step
Action
1
Press Stop. The run is now stopped. To indicate that the run has stopped,
Idle is displayed below the instrument control buttons.
2
The run is now stopped which is indicted by the word Idle below the instrument control buttons.
Note:
While using Stop it is not possible to start the protocol again except from the
beginning of the protocol.
Display graphics and data
Ettan IPGphor 3 Control Software provides graphics of the data (Voltage, Volt-hours and
Current) as it is retrieved from Ettan IPGphor 3.
The user can monitor and display the progress of any individual run from any one of
four different instruments being run in parallel.
98
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.4 Run a pre-programmed protocol from the PC
5.4.2 Perform a run
Monitor a run
Follow the instructions below to monitor the run.
Step
Action
1
Select the correct instrument selection button (Instr1 - Instr4) to select the
desired instrument. It is possible to monitor and display the progress of any
individual run from any one of four different instruments being run in parallel.
The identity of the selected instrument is shown below the instrument control
buttons.
2
Open the Run settings & details tab by clicking the tab heading.
3
In the Protocol details graph two of three possible parameters (setpoint
voltage, measured voltage and measured current) are displayed to give an
overview of the protocol and the run.
4
Open any of the combo boxes below the graph and then click on the desired
parameter to be displayed in the Protocol details graph.
Note:
If measured voltage and measured current are selected, the time scale
changes and only the elapsed time is shown.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
99
5 Operation
5.4 Run a pre-programmed protocol from the PC
5.4.2 Perform a run
Step
Action
5
Click the left of the two buttons at the lower right corner of the tab window
to select the Info and data view.
The main running parameters are now displayed. Vh or Time is indicated
depending on what is selected to define the length of the current step.
6
Open the Session log tab by clicking the tab heading to view the running
data collected in the log file.
The session log file stores instrument identity, User ID, current protocol, start
time and many other parameters, please refer to Ettan IPGphor 3 Control
Software User Manual for details.
100
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
5 Operation
5.4 Run a pre-programmed protocol from the PC
5.4.2 Perform a run
Step
Action
7
Click another instrument selection button for monitoring runs on other instruments controlled from the software. This way it is possible to monitor
up to four parallel runs.
Capture run data
Run data is acquired from the Ettan IPGphor 3 Isoelectric Focusing Unit automatically
every minute during a run, and the data is presented in a graph and in numerical displays.
Store data
All run data and the session log can be exported to a Microsoft Excel file. It is also possible
to save the current application window as an image file.
Follow the instructions below to export data.
Step
Action
1
Select Export:File.
2
Select file. The default file name is the date and time (e.g. Apr 11, 2013
10.56.45).
3
Select location. The default location for the saved file is C:\IPGphor3\RunData.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
101
6 Maintenance
6
Maintenance
Precautions
WARNING
The customer must make sure that all installation, maintenance,
operation and inspection is carried out by qualified personnel who
are adequately trained, understand and adhere to local regulations
and the operating instructions, and have a thorough knowledge
of the product and the entire process.
WARNING
Ettan IPGphor 3 covers must not be opened by the user. It contains
electrical circuits which can give a lethal electric shock. Service
and planned maintenance should be performed by personnel authorized by GE.
WARNING
Disconnect power. Always disconnect power from the instrument
before performing any maintenance task.
WARNING
Do not remove the main cover. There are no userserviceable
components inside, and you can be exposed to high voltage.
NOTICE
Keep the instrument dry and clean. Wipe regularly with a soft damp
tissue and, if necessary, a mild cleaning agent. Let the instrument
dry completely before use.
102
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
6 Maintenance
6.1 Cleaning Ettan IPGphor 3
6.1
Cleaning Ettan IPGphor 3
Precautions
WARNING
If large volumes of liquid have penetrated the casing of the instrument and come into contact with the electrical components, immediately switch off the instrument and contact an authorized service
technician.
NOTICE
Do not use strong acids, bases, ketones, alcohols, or other reagents
to clean the covers, sample cups, or electrodes or the parts may
be damaged.
Cups may be briefly rinsed with ethanol if desired.
NOTICE
Clean strip holders and manifold with the strip holder cleaning solution provided. Clean all other components that come in contact
with the IPG strip or the sample with a detergent designed for
glassware. Rinse well with distilled water.
Clean Ettan IPGphor 3
The parts of the instrument that come in contact with liquid reagents are resistant to
chemicals typically used for IEF. Before introducing any other chemicals into the system,
first test the affected parts.
Cleaning procedures
IPGphor Strip Holder Cleaning Solution has been specifically formulated for removing
protein deposits. It will not damage the strip holder or manifold. IPGphor Strip Holder
Cleaning Solution (29-0119-27) can be ordered in 1 liters bottles from GE.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
103
6 Maintenance
6.1 Cleaning Ettan IPGphor 3
Clean ceramic strip
holders/manifold
NOTICE
The ceramic strip holders and manifold are very brittle and fragile.
Take care in handling and do not subject to impacts.
The Ettan IPGphor 3 strip holders and manifold are made from a thermally conductive
aluminum oxide ceramic. They have been surface-treated to minimize adsorption and
interaction with proteins. The surface coating is mechanically robust and can withstand
temperatures of up to 300ºC, however it can be damaged by prolonged exposure to
extremes of pH.
Clean strip holders/manifold after each first-dimension IEF run. Do not let solutions dry
in the strip holder/manifold. Cleaning may be more effective if the strip holder/manifold
is first soaked a few hours or overnight in a solution of 2% to 5% IPGphor Strip Holder
Cleaning Solution in water. Strip holders and manifold may also be immersed in boiling
1% SDS.
NOTICE
You must use a neutral pH detergent, such as the IPGphor Strip
Holder Cleaning Solution, to remove residual protein from the strip
holders/manifold.
NOTICE
Fixed length strip holders and manifold may be baked, boiled or
autoclaved. DO NOT EXPOSE THEM TO STRONG ACIDS OR BASES,
INCLUDING ALKALINE DETERGENTS.
Instruction
104
Step
Action
1
First rinse off the strip holder/manifold. Use a mild liquid soap to remove
any residual IPG cover fluid.
2
Squeeze a few drops of IPGphor Strip Holder Cleaning Solution into the strip
holder/manifold slot. Use a toothbrush and vigorous agitation to clean the
strip holder/manifold.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
6 Maintenance
6.1 Cleaning Ettan IPGphor 3
Step
Action
3
Rinse well with distilled or deionized water.
4
Thoroughly air dry the strip holders/manifold or dry well with a lint-free tissue
prior to use.
5
Wipe the platform and plate electrodes with a lab tissue or paper towel after
each use. Other exterior surfaces can be cleaned with a damp cloth. Never
use abrasive cleanser or solvents.
Clean Manifold Light
Clean Ettan IPGphor Manifold Light with water, no warmer than 40°C, and with IPGphor
Strip Holder Cleaning Solution.
CAUTION
Do not use any organic solvents during cleaning.
Clean electrodes
NOTICE
Avoid scratching the electrode surfaces.
Wash the electrode assemblies with the IPGphor Strip Holder Cleaning Solution. Rinse
thoroughly with water then deionized water and allow to air dry.
Cleaning before planned
maintenance/service
To ensure the protection and safety of service personnel, all equipment and work areas
must be clean and free of any hazardous contaminants before a Service Engineer starts
maintenance work.
Please complete the checklist in the On Site Service Health and Safety Declaration Form
or the Health and Safety Declaration Form for Product Return or Servicing, depending on
whether the instrument is going to be serviced on site or returned for service, respectively.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
105
6 Maintenance
6.1 Cleaning Ettan IPGphor 3
Copy the form you need from Section 8.1 Health and Safety Declaration Form, on page 112
or print it from the PDF file available on the User Documentation CD.
106
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
6 Maintenance
6.2 Replace fuses
6.2
Replace fuses
Precautions
WARNING
Always disconnect power from the instrument and detach the
power cord before replacing fuses.
Fuses
Fuses protect equipment by disconnecting loads too large for the instrument’s circuit
design, so it is imperative that fuses are replaced only with fuses of identical rating. The
mains power module, located at the back of the instrument, contains two input mains
fuses which are the same for all models: T2.5 AL 250V, 5 × 20 mm.
Fuse compartment
3
1
4
2
Part
Function
1
Mains power switch
2
Hinged cover
3
Slot
4
Casette end
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
107
6 Maintenance
6.2 Replace fuses
Instruction for replacing fuses
108
Step
Action
1
Open the fuse compartment by inserting a small flat-blade screwdriver into
the slot at the top of the power module. Twist the screwdriver 1/8 – turn to
release the cover, then pull out the hinged compartment, which opens out.
2
Insert the screwdriver above the arrow on one fuse cassette, catch the
cassette end, and slowly slide it completely out of the module.
3
Pull the fuse out of its cassette and inspect. If the fuse element is burned or
broken, replace the fuse with an identical type. If the fuse appears to be intact, check it with a multi-meter. (A reading of 1 ohm or less indicates the
fuse is still usable.)
4
After placing a new fuse into the cassette, slide it into the power module,
making sure the arrow on the cassette points to the right (in the same direction as the guide arrows on the inside of the compartment door).
5
Repeat steps 2 to 4 for the second cassette.
6
Close the fuse compartment cover and gently press it into the power module
until it snaps shut.
7
Plug the power cord into the unit and turn the mains power switch on.
8
If the instrument does not start when power is switched on after replacement
of mains fuse(s), call GE service.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
7 Troubleshooting
7
Troubleshooting
Troubleshooting guide
The following table specifies the troubleshooting situations that you might encounter
while using Ettan IPGphor 3.
Symptom
Possible solution
Problems indicated by LCD messages
Lid open step 1, close to continue
The safety lid is not properly closed. When the
safety lid is open, the system has an automatic
voltage cutoff safety feature. In order for the protocol to proceed, the safety lid must be closed.
Locked screen in edit mode
Turn off the mains power switch to reset the instrument.
Blank display
If no electrical components are functioning (e.g.
HV ON lamp does not light and the cooling fans
are motionless), check the fuses in the mains
power module.
Diagnostic program indicates
component failure
Note the component that failed and press the
START key to continue through the diagnostic
program. Call your local GE service for further information on how to remedy the failure.
Arc
An electric arc has been detected. The instrument
will reduce the voltage setting 500 V automatically. The run continues, and there is no need for
further action.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
109
7 Troubleshooting
Symptom
Possible solution
Power delivery
Current too low or zero
At least two of three pressure pads under the
safety lid should press gently against the strip
holders to ensure electrical continuity between
the strip holder electrodes and the electrode areas
on the platform.
The gel must be evenly and completely rehydrated
to conduct current. Make sure the proper amount
of rehydration solution is applied to the IPG strip
holder and allow a minimum of 10 hours for rehydration.
Check that both connecting points of the strip
holder or the connecting pins of the manifold
electrodes are in contact with the gold surface.
Voltage limit not reached
The ionic strength of the rehydration solution is
too high. The instrument has reached the current
limit; reduce the IPG buffer concentration; use a
mixed-bed ion-exchange resin to remove ionic
breakdown products of urea of other additives.
Desalt the sample or prepare the sample so that
the salt concentration is less than 10 mM.
Sparks or burning in strips
Reduce the current limit. Do not exceed 50 μA per
strip.
Prevent the IPG strip from drying out by always
applying Immobiline DryStrip Cover Fluid during
rehydration.
Ensure that the IPG strip is fully rehydrated along
the entire length of the strip. The IPG strip should
be in complete contact with the correct volume
of rehydration solution. Remove any air bubbles
trapped under the IPG strip.
Desalt the sample or prepare the sample so that
the salt concentration is less than 10 mM. Deionize additives to the rehydration solution. Excessive charged material in the sample or rehydration
buffer lead to electro-endosmosis which will dry
out the middle of the strip, possibly leading to
arcing and burning at these points.
110
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
8 Reference information
8
Reference information
Technical service and repair
GE offers complete technical support for all our products. If you have any questions
about how to use this product, or would like to arrange to repair it, please call or fax
your local GE representative.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
111
8 Reference information
8.1 Health and Safety Declaration Form
8.1
Health and Safety Declaration Form
On site service
On Site
e Servicce Health &
Safety
y Declarration Form
F
Serv
vice Ticket #
#:
To make the mutual protection an
nd safety of GE
E service persoonnel and our customers,
c
all equipment an
nd work areas must be
a free of anyy hazardous contaminants
c
before
b
a Servicce Engineer starts a repair. To
T avoid delayss in the servicing of your
clean and
equipm
ment, please ccomplete this checklist
c
and present
p
it to thee Service Engineer upon arrival. Equipmen
nt and/or work
k areas
not sufficiently clean
ned, accessible
e and safe for an engineer m
may lead to delays in servicin
ng the equipment and could be subject to
onal charges.
additio
Yes
No
P
Please review
w the actions below
b
and ansswer “Yes” or “No”.
P
Provide explanation for any
y “No” answeers in box belo
ow.
IInstrument ha
as been cleaned of hazardo
ous substance
es.
P
Please rinse tu
ubing or piping
g, wipe down sscanner surfacces, or otherw
wise ensure rem
moval of any dangerous
rresidue. Ensure the area aro
ound the instru
ument is clean. If radioactivitty has been ussed, please perform a
w
wipe test or otther suitable survey.
A
Adequate space and clearance is provideed to allow safe access for in
nstrument servvice, repair or
iinstallation. In some cases this may requirre customer to
o move equipm
ment from norrmal operating
g location
p
prior to GE arrrival.
C
Consumabless, such as colu
umns or gels, h
have been rem
moved or isola
ated from thee instrument and
a from
a
any area thatt may impede access to thee instrument .
A
All buffer / wa
aste vessels are labeled.
E
Excess containers have bee
en removed fr
from the area to provide acccess.
Provide
expla
anation
for an
ny “No”
answ
wers here:
Equip
pment type / Product No:
Serial No:
I here
eby confirm th
hat the equipm
ment specified above has beeen cleaned to remove any hazardous
h
sub
bstances and that the
area has been mad
de safe and acccessible.
Name:
Comp
pany or institu
ution:
Posittion or
job tiitle:
Date (YYYY/MM/DD
D):
Signe
ed:
GE and GE monogram are trade
emarks of General EElectric Company.
Avenue, P.O. Box 1327, Piscataway,
GE Healthcare Bio-Sciences Corp, 800 Centennial A
NJ 08855-1
1327
© 2010-14 General Electric Com
mpany—All rights resserved. First publishe
ed April 2010.
D
DOC1149542/28-980
00-26 AC 05/2014
112
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
8 Reference information
8.1 Health and Safety Declaration Form
Product return or servicing
Healtth & Safeety Declaration
n Form
for Prroduct Return
R
oor Servic
cing
Return authorization
numb
ber:
and/or
SService Tickett/Request:
To make sure the mu
utual protectio
on and safety of GE personn
nel, our custom
mers, transportation personn
nel and our en
nvironment,
all equ
uipment must be clean and free
f
of any hazzardous conta
aminants before shipping to GE. To avoid d
delays in the processing of
your equipment, please complete this checklist and include it with your retu
urn.
1.
2.
3.
Please notee that items will NOT be acce
epted for servi cing or return without this fo
orm
Equipment which is not sufficiently clea
aned prior to rreturn to GE may
m lead to delays in servicin
ng the equipment and
could be subject to additional charges
Visible contamination will be assumed hazardous and
d additional clleaning and de
econtaminatio
on charges will be applied Yes
No
P
Please specifyy if the equipm
ment has been
n in contact wiith any of the following:
R
Radioactivity (p
please specify)
In
nfectious or ha
azardous biolo
ogical substan ces (please sp
pecify)
O
Other Hazardo
ous Chemicals (please specifyy)
Equip
pment must be
e decontamin
nated prior to service / retu rn. Please pro
ovide a telephone number w
where GE can contact
you fo
or additional iinformation concerning the
e system / equ
uipment.
Telep
phone No:
Wateer
Liquid and/or gas in equipmentt is:
Ethan
nol
Nonee, empty
Argo n, Helium, Nittrogen
Liqui d Nitrogen
Other, please
specify
Equip
pment type / P
Product No:
Serial No:
I here
eby confirm th
hat the equipm
ment specified above has b
been cleaned to
t remove any
y hazardous ssubstances an
nd that the
area has been mad
de safe and accessible.
Name:
Comp
pany or
instittution:
Posittion or job title
e:
Date (YYYY/MM/DD
D)
Signe
ed:
To receive a return authorization
n number or service numbeer,
please
e call local tecchnical support or custome
er service.
GE and GE monogram are trade
emarks of General EElectric Company.
Avenue, P.O. Box 1327, Piscataway,
GE Healthcare Bio-Sciences Corp, 800 Centennial A
NJ 08855-1
1327, US
© 2010-14 General Electric Com
mpany—All rights resserved. First publishe
ed April 2010.
D
DOC1149544/28-980
00-27 AC 05/2014
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
113
8 Reference information
8.2 Technical specifications
8.2
Technical specifications
Power specifications
Parameter
Specification
Mains supply voltage
100-240 V AC ±10%
Frequency
50/60 Hz
Power consumption
230 VA
Transient overvoltages
Overvoltage category II
Electrophoresis
Parameter
Specification
High voltage
Up to 10 kV
Current
Up to 1.5 mA
Working surface
Parameter
Specification
Electrode areas
Gold-plated copper.
Capacity
Up to 12 fixed-length strip holders or one manifold.
Platform temperature
15°C to 30°C ±2°C.
User interface
114
Item/Parameter
Specification
Control panel
7-key membrane keypad.
Liquid crystal display (LCD)
4 lines, 24 characters per line.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
8 Reference information
8.2 Technical specifications
Item/Parameter
Specification
Programmable parameters
Rehydration time, platform temperature,
maximum current limit per strip, voltage limit
in each step, voltage step or gradient, and
step duration.
Protocol capacity
10 protocols, with up to 9 steps each.
Serial port
RS232, 1200 or 9600 baud, 8 data bits 1 stop
bit, no parity, no flow control. 9600 baud is
mandatory for connection with
Ettan IPGphor 3 Control Software.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
115
8 Reference information
8.3 Ordering information
8.3
Ordering information
The following table provides you with ordering information.
Replacements
Replacement part
Code number
Ettan IPGphor Cup Loading Manifold, ceramic
80-6498-57
Ettan IPGphor Cup Loading Manifold Light
11-0025-80
Sample cups, pack of 20
(6x)
80-6498-95
Paper electrode wicks,
pack of 40 (6x)
80-6499-14
Paper bridge pads, pack
of 20 (6x)
80-6499-33
Electrode set
80-6498-76
Cleaning brush
80-6505-98
Spirit level
80-6194-19
Forceps SS
80-6506-17
Accessories
116
Accessory part
Quantity
Code number
Immobiline DryStrip Reswelling
Tray, 7–18 cm
1
80-6371-84
Immobiline DryStrip Reswelling
Tray, 7–24 cm
1
80-6465-32
Immobiline DryStrip Cover Fluid
1 liter
17-1335-01
IPGphor Strip Holder Cleaning Solution
1 liter
29-0119-27
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
8 Reference information
8.3 Ordering information
More information
For more details on ordering information and for information about spare parts and
accessories, visit www.gelifesciences.com/2DE or contact your local GE representative.
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
117
Index
Index
A
Gradient, 72
Accessories, 44
Assembly, 44
I
B
Baud rate, 43
C
CE
conformity, 9
CE marking, 10
Ceramic manifold, 35
Cleaning, 103
Command keys, 69
Control panel, 29–30
Edit step or gradient, 79
IEF steps screen, 72
Protocol identification
screen, 71, 75
Running screen, 85
Select a protocol, 83
Stop a protocol, 89
Control software, 34
D
Diagnostic program, 42, 66
Documentation
Associated, 12
E
Electrode area, 29
Electrode pads, 53
Electrode paper wicks, 63
Electrodes, 64
Clean, 105
Emergency shutdown, 22
F
FCC compliance, 10
Fuse holder, 31
Fuses
Replace, 108
G
General Precautions, 14
118
IEF programming screen, 72
Immobiline DryStrip
Cover fluid, 52
Rehydrate, 59
Immobiline DryStrip
Rehydrate, 49
Instrument, 28
International Standard, 10
IPGbox, 59
IPG strip manifold, 35
IPG strips, 51
Position, 61
L
LCD screen, 30, 67
Lid adapter, 33
Light manifold, 36
M
Main screen
Software, 91
Manifold, 35, 60
Ceramic, 35
Light, 36
Manifold ceramic
Clean, 104
Manifold light
Clean, 105
Manufacturing information, 9
O
Operating environment, 38
P
Personal protection, 15
Platform, 32, 60
Power connection, 42
Power failure, 22
Power inlet, 31
Power supply, 39, 114
Power switch, 31
Protective cover, 32
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
Index
Protocol identification
screen, 71, 75
Protocol number, 83
Protocol parameters, 73
Protocol running screen, 85
Protocol step screen, 79
Protocol verification, 81
R
Regulatory compliance of connected equipment, 11
Rehydration, 52
S
Safety lid, 29, 32, 56, 109
Safety precautions, 14
General precautions, 14
Sample, 65
Sample cups, 62
Serial port, 42
Session log, 100
Software, 34
Capture run data, 101
Ettan IPGphor 3 Operating Instructions 28-9635-51 AC
Edit protocol, 94
Monitor a run, 99
Pause a run, 98
Save protocol, 95
Select instrument, 92
Set up run, 93
Start run, 97
Stop a run, 98
Store data, 101
Spare parts, 44
Specifications, 38
Step, 72
Strip holder, 48
Preparation, 48
Strip holders
Position, 55
T
Transport, 40
Typographical conventions, 6
U
Unpacking, 40
119
For local office contact information, visit
www.gelifesciences.com/contact
GE and GE monogram are trademarks of General Electric Company.
GE Healthcare UK Limited
Amersham Place
Little Chalfont
Buckinghamshire, HP7 9NA
United Kingdom
© 2005-2015 General Electric Company – All rights reserved.
First published 2005
www.gelifesciences.com/2DE
CyDye, Ettan, Immobiline and IPGphor are trademarks of General Electric Company or one of its subsidiaries.
All goods and services are sold subject to the terms and conditions of sale of
the company within GE Healthcare which supplies them. A copy of these terms
and conditions is available on request. Contact your local GE Healthcare representative for the most current information.
GE Healthcare Bio-Sciences AB
Björkgatan 30, 751 84 Uppsala, Sweden
GE Healthcare Europe GmbH
Munzinger Strasse 5, D-79111 Freiburg, Germany
GE Healthcare Bio-Sciences Corp.
800 Centennial Avenue, P.O. Box 1327, Piscataway, NJ 08855-1327, USA
GE Healthcare Japan Corporation
Sanken Bldg. 3-25-1, Hyakunincho Shinjuku-ku, Tokyo 169-0073, Japan
28-9635-51 AC 02/2015 a24
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