Bruker MBT Galaxy HCCA Matrix IVD Matrix Substance Instructions for Use

Instructions for Use

MBT Galaxy HCCA Matrix IVD

Purified matrix substance for matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF-MS).

For use with MBT Galaxy (# 1836007) in the preparation workflow for the analysis of clinical samples from human specimens.

CARE products are designed to support our worldwide customers with highquality consumables, accessories and dedicated kits.

The CARE product range is specifically optimized and certified for use with all

Bruker Daltonics systems.

www.bruker.com/care

Revision C (December 2015)

Language: en

Bruker

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Intended Use

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Product Description

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Inspection, Storage and Stability

3.1

Inspection on Arrival

3.2

Storage on Arrival

3.3

Storage after Solubilization

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Risk and Safety Information

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Field of Application

6

Solubilization of Galaxy HCCA

7

Sample Preparation Using Galaxy HCCA

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Manufacturer

1 Intended Use

MBT Galaxy HCCA Matrix IVD is used as the MALDI matrix for MALDI- TOF mass spectrometric analysis of clinical samples from human specimens in conjunction with the MBT Galaxy (# 1836007).

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2 Product Description

MBT Galaxy HCCA Matrix IVD (referred to as 'Galaxy HCCA'; HCCA = α-Cyano-4-hydroxycinnamic acid) enables easy and convenient preparation of MALDI matrix solution for MALDI- TOF- MS measurement of peptides and proteins. Each vial contains 7.5 ±0.3 mg matrix, enabling fast preparation of the desired concentration by adding the relevant volume of solvent.

Synonyms 2-Cyano-3-(4-hydroxyphenyl) acrylic acid

Molecular formula C

10

H

7

NO

3

Structural formula

Molecular weight 189.17 g/mol

CAS number 28166-41-8

EC number

Melting point

248-879-1

245-250°C

Content 7.5 mg Galaxy HCCA / vial.

This kit is an in-vitro diagnostic device.

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Ordering Information

Product

MBT Galaxy HCCA Matrix IVD, 10 vials

Part Number

# 1827419

3 Inspection, Storage and Stability

3.1

Inspection on Arrival

Check the Galaxy HCCA package on arrival. If it is damaged, check the vials. If the vials are damaged, the Galaxy HCCA must not be used. Dispose of the Galaxy HCCA (vials and package) following the guidelines outlined in the Material Safety Data Sheet for the product and contact Bruker Daltonik

GmbH Service Department ([email protected]) for a replacement.

3.2

Storage on Arrival

+8°C Galaxy HCCA is shipped at ambient temperature.

The expiry date on the package is valid for the product when stored in a refrigerator at 2°C to 8°C on arrival.

+2°C

3.3

Storage after Solubilization

+25°C Dissolved Galaxy HCCA is stable at room temperature

(20–25°C) for up to one week.

+20°C

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4 Risk and Safety Information

MBT Galaxy HCCA Matrix IVD is classified as a hazardous chemical:

WARNING (H315, H319, H335) 1

For more information, read the Material Safety Data Sheet available for download at www.bruker.com/msds.

Additional chemicals may be required for procedures described in these Instructions for Use. Carefully read the Material Safety Data Sheet provided by the supplier and follow general safety regulations when handling chemicals or biohazardous material.

5 Field of Application

Galaxy HCCA enables easy and convenient preparation of HCCA matrix solutions using the MBT

Galaxy. The matrix is soluble in standard solvent (see section 6), is easy to handle, and enables highly

sensitive MALDI-TOF-MS measurement of peptides and proteins from 0.7 to 20 kDa.

MALDI-TOF-MS using HCCA as a matrix has been shown to be a reliable, high-throughput method for the classification and identification of microorganisms.

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H315: Causes skin irritation; P264: Wash hands thoroughly after handling; P280: Wear protective gloves/protective clothing/eye protection/face protection; P302+P352: IF ON SKIN: Wash with soap and water; P321: Specific treatment (see First aid measures in the Material Safety Data Sheet);

P332+P313 If skin irritation occurs: Get medical advice/attention; P362+P364: Take off contaminated clothing and wash it before reuse.

H319: Causes serious eye irritation; P264: Wash hands thoroughly after handling; P280: Wear protective gloves/protective clothing/eye protection/face protection; P305+P351+P338: IF IN EYES:

Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do.

continue rinsing; P337+P313 If eye irritation persists get medical advice/attention.

H335: May cause respiratory irritation; P261: Avoid breathing dust/fume/gas/mist/vapors/spray; P271:

Use only outdoors or in a well-ventilated area; P304+P340: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing; P312: Call a POISON CENTER or doctor/physician if you feel unwell; P403+P233: Store in a well ventilated place. Keep container tightly closed; P405: Store locked up; P501: Dispose of contents/container in accordance with local/regional/national/international regulations.

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6 Solubilization of Galaxy HCCA

Chemicals and Materials Required

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Standard solvent (acetonitrile 50%, water 47.5% and trifluoroacetic acid 2.5%) from Sigma-

Aldrich (# 19182) or OS solution from VWR (# PRLS89449.230), which have been tested by

Bruker Daltonik GmbH and are recommended for reconstitution of Galaxy HCCA.

Equipment and Tools Required

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Vortex mixer, pipette, pipette tips

Solubilization Procedure

The procedure for solubilization of Galaxy HCCA depends on the type of MALDI target plate to be used.

For BigAnchor targets

1. Add 1.5 mL standard solvent to the screw-cap vial containing Galaxy HCCA (final concentration:

5 mg Galaxy HCCA/mL) and close the vial tightly.

2. Shake the screw-cap vial and use a vortex mixer to completely dissolve Galaxy HCCA at room temperature. Finally, shake the contents down and tap the closed vial on a tabletop so that any liquid that has collected inside the screw cap falls into the vial.

3. Remove the screw cap and exchange the matrix vial in Channel 1 of the MBT Galaxy liquids drawer as described in the MBT Galaxy User Manual.

For all other targets approved for your workflow

1. Add 750 µL standard solvent to the screw-cap vial containing Galaxy HCCA (final concentration:

10 mg Galaxy HCCA/mL) and close the vial tightly.

2. Shake the screw-cap vial and use a vortex mixer to completely dissolve Galaxy HCCA at room temperature. Finally, shake the contents down and tap the closed vial on a tabletop so that any liquid that has collected inside the screw cap falls into the vial.

3. Remove the screw cap and exchange the matrix vial in Channel 1 of the MBT Galaxy liquids drawer as described in the MBT Galaxy User Manual.

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7 Sample Preparation Using Galaxy HCCA

The procedures below can be used for the classification and identification of microorganisms through

MALDI-TOF-MS.

CAUTION If Galaxy HCCA matrix solution is not added to samples within 30 minutes after they have dried, these positions cannot be tested.

Direct transfer sample preparation procedure

1. For each sample, smear an isolated colony as a thin film directly onto a sample position using a sterile wooden stick.

2. Deposit 1 μL of IVD BTS onto at least one MALDI target plate position.

3. Allow the sample spots to dry at room temperature.

4. Perform the matrix only MBT Galaxy preparation workflow as described in the MBT Galaxy User

Manual.

5. Perform MALDI-TOF-MS measurement.

extended Direct Transfer sample preparation procedure

1. For each sample, smear an isolated colony as a thin film directly onto a sample position using a sterile wooden stick.

2. Deposit 1 μL of IVD BTS onto at least one MALDI target plate position.

3. Allow the sample spots to dry at room temperature.

4. Perform the formic acid + matrix MBT Galaxy preparation workflow as described in the MBT

Galaxy User Manual.

5. Perform MALDI-TOF-MS measurement.

Extraction sample preparation procedure

1. Follow the extraction sample preparation procedure up to the point where the supernatant is deposited onto the MALDI target plate.

2. Deposit 1 μL of IVD BTS onto at least one MALDI target plate position.

3. Allow the sample spots to dry at room temperature.

4. Perform the matrix only MBT Galaxy preparation workflow as described in the MBT Galaxy User

Manual.

5. Perform MALDI-TOF-MS measurement.

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8 Manufacturer

Bruker Daltonik GmbH

Fahrenheitstraße 4

28359 Bremen

Germany

Support

E-mail: [email protected]

Phone: +49 (421) 2205-1401

Fax: +49 (421) 2205-106

Sales Information

E-mail: [email protected]

Phone: +49 (421) 2205-1402

Web: www.bruker.com

# 1827419

Descriptions and specifications supersede all previous information and are subject to change without notice.

© Copyright 2015 Bruker Daltonik GmbH

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