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Operating Instructions
CO
2
Incubator
MCO-230AICUVL
This product is for research use only.
Not for clinical diagnosis or treatment of humans or animals.
Please read the operating instructions carefully before using this product, and save the operating instructions for future use.
See page 92 for all model numbers.
CONTENTS
INTRODUCTION ........................................................................................... 3
PRECAUTIONS FOR SAFE OPERATION .................................................... 4
LABELS ON INCUBATOR ............................................................................ 9
ENVIRONMENTAL CONDITIONS ............................................................... 10
Unit ........................................................................................................... 11
LCD touch panel ....................................................................................... 13
Remote alarm terminal ............................................................................. 15
INSTALLATION
Installation site .......................................................................................... 16
Installation ................................................................................................ 18
Connecting CO
2
gas cylinder ................................................................... 21
Initial cleaning method ............................................................................. 22
Removing inner attachments .................................................................... 23
Installing inner attachments ...................................................................... 25
Filling humidifying pan .............................................................................. 26
Precautions for cultures ........................................................................... 27
Preventing contamination ........................................................................ 28
CORRECT OPERATION ............................................................................. 29
BASIC OPERATION ON LCD TOUCH PANEL ........................................... 30
Numerical input to input window .............................................................. 32
Setting temperature, CO
2
density and high limit temperature alarm ............. 33
Setting key lock ........................................................................................ 35
Removing key lock ................................................................................... 37
ALARM PARAMETERS ............................................................................... 39
Setting log interval .................................................................................... 42
Displaying operation log ........................................................................... 43
Exporting operation log ............................................................................ 46
Displaying alarm log ................................................................................. 49
Exporting alarm log .................................................................................. 51
Setting date and time ............................................................................... 54
Setting brightness and sleep .................................................................... 55
1
CONTENTS
UV LAMP PARAMETERS ............................................................................ 57
Using UV lamp ......................................................................................... 57
Setting UV lamp ON period ...................................................................... 58
Lighting UV lamp for 24 hours .................................................................. 60
H
2
O
2
DECONTAMINATION ......................................................................... 62
H
2
O
2
decontamination .............................................................................. 63
Precautions when handling H
2
O
2
reagent ................................................ 67
Using unlock key ...................................................................................... 67
ELECTRIC LOCK (OPTION) ....................................................................... 68
Setting User-ID ......................................................................................... 68
Setting auto lock ....................................................................................... 70
Using unlock key ...................................................................................... 73
Removing auto lock .................................................................................. 74
GAS AUTO CHANGER (OPTION) .............................................................. 75
Connecting CO
2
gas cylinder ................................................................... 75
Automatic CO
2
gas supply line changeover ............................................. 76
Manual CO
2
gas supply line changeover ................................................. 78
STD GAS AUTO CALIBRATION KIT (OPTION) .......................................... 79
ROUTINE MAINTENANCE.......................................................................... 82
ALARMS, SAFETY, AND SELF-DIAGNOSIS .............................................. 83
TROUBLESHOOTING ................................................................................. 86
DISPOSAL OF UNIT .................................................................................... 89
SPECIFICATIONS ....................................................................................... 90
PERFORMANCE ......................................................................................... 92
SAFETY CHECK SHEET ............................................................................ 93
2
INTRODUCTION
■ Read the operating instructions carefully before using the Product and follow the instructions for safety operation.
■ PHC Corporation disavows any responsibility for safety if the Product is used for other than the intended use or used with any procedures other than those given in the operating instructions.
■ Keep the operating instructions in a suitable place so that it can be referred to as necessary.
■ The contents of the operating instructions are subject to change without notice for improvement of performance or functions.
■ Contact our sales representative or agent if any page of the operating instructions is lost or the page order is incorrect.
■ Contact our sales representative or agent if any point in the operating instructions is unclear or if there are any inaccuracies.
■ No part of the operating instructions may be reproduced in any form without the expressed written permission of PHC Corporation.
IMPORTANT NOTICE
PHC Corporation guarantees this product under certain warranty conditions. However, please note that
PHC Corporation shall not be responsible for any loss or damage to the contents of the product.
<Intended Use>
This equipment is designed for cell and tissue culture for laboratory use. Not for clinical diagnosis or treatment of humans or animals.
3
PRECAUTIONS FOR SAFE OPERATION
It is imperative that the user complies with the operating instructions as it contains important safety advice.
Items and procedures are described so that you can use this unit correctly and safely. If the precautions advised are followed, this will prevent possible injury to the user and any other person.
Precautions are illustrated in the following way:
WARNING
Failure to observe WARNING signs could result in a hazard to personnel possibly resulting in serious injury or death.
CAUTION
Failure to observe CAUTION signs could result in injury to personnel and damage to the unit and associated property.
Symbol shows;
This symbol means caution.
This symbol means an action is prohibited.
This symbol means an instruction must be followed.
Be sure to keep the operating instructions in a place accessible to users of this unit.
WARNING
As with any equipment that uses CO
2
gas, there is a likelihood of oxygen depletion in the vicinity of the equipment. It is important that you assess the work site to ensure there is suitable and sufficient ventilation. If restricted ventilation is suspected, then other methods of ensuring a safe environment must be considered. These may include atmosphere monitoring and warning devices.
4
USA Only (Model with a lamp): This product has a lamp that contains mercury. Disposal may be regulated in your community due to environmental considerations.
Contains mercury / Contenu avec mercure
For more information on safe handling Pour plus de renseignements sur les procedures, the measures to be taken in procédures de manutention sécuritaire, les case of accidental breakage and safe disposal options visit: ec.gc.ca/mercure-mercury/.
Dispose of or recycle in accordance with applicable laws. mesures à prendre en cas de bris accidentel et les options d’élimination sécuritaire visitez: ec.gc.ca/mercure-mercury/.
Mettez au rebut ou recyclez conformément aux lois applicables.
For the State of California, USA Only:
This product contains a CR Coin Cell Lithium Battery which contains Perchlorate Material – special handling may apply. See www.dtsc.ca.gov/hazardouswaste/perchlorate.
5
PRECAUTIONS FOR SAFE OPERATION
WARNING
Do not use the unit outdoors.
Current leakage or electric shock may result if the unit is exposed to rain water.
Only qualified engineers or service personnel should install the unit.
The installation by unqualified personnel may cause electric shock or fire.
Install the unit on a sturdy floor and take an adequate precaution to prevent the unit from turning over.
If the floor is not strong enough or the installation site is not adequate, this may result in injury from the unit falling or tipping over.
Never install the unit in a humid place or a place where it is likely to be splashed by water.
Deterioration of the insulation may result which could cause current leakage or electric shock.
Never install the unit in a flammable or volatile location.
This may cause explosion or fire.
Never install the unit where acid or corrosive gases are present as current leakage or electric shock may result due to corrosion.
Always ground (earth) the unit to prevent electric shock. If the power supply outlet is not grounded, it will be necessary to install a ground by qualified engineers.
Never ground the unit through a gas pipe, water main, telephone line or lightning rod.
Such grounding may cause electric shock in the case of an incomplete circuit.
Connect the unit to a power source as indicated on the rating label attached to the unit. Use of any other voltage or frequency other than that on the rating label may cause fire or electric shock.
Never store volatile or flammable substances in this unit if the container cannot be sealed. These may cause explosion or fire.
Do not insert metal objects such as a pin or a wire into any vent, gap or any outlet on the unit.
This may cause electric shock or injury by accidental contact with moving parts.
Use this unit in safe area when treating the poison, harmful or radiate articles.
Improper use may cause bad effect on your health or environment.
Turn off the power switch (if provided) and disconnect the power supply to the unit prior to any repair or maintenance of the unit in order to prevent electric shock or injury.
Do not touch any electrical parts (such as power supply plug) or operate switches with a wet hand.
This may cause electric shock.
6
WARNING
Ensure you do not inhale or consume medication or aerosols from around the unit at the time of maintenance. These may be harmful to your health.
Never splash water directly onto the unit as this may cause electric shock or short circuit.
Never put containers with liquid on the unit as this may cause electric shock or short circuit when the liquid is spilled.
Never bind, process, or step on the power supply cord, or never damage or break the power supply plug.
A broken power supply cord or plug may cause fire or electric shock.
Do not use the power supply cord if its power supply plug is loose. Such power supply cord may cause fire or electric shock.
Never disassemble, repair, or modify the unit yourself.
Any such work carried out by an unauthorized person may result in fire, or electric shock or injury due to a malfunction.
Disconnect the power supply plug if there is something wrong with the unit.
Continued abnormal operation may cause electric shock or fire.
When removing the power supply plug from the power supply outlet, grip the power supply plug, not the cord. Pulling the cord may result in electric shock or fire by short circuit.
Disconnect the power supply plug before moving the unit. Take care not to damage the power supply cord. A damaged cord may cause electric shock or fire.
Disconnect the power supply plug when the unit is not used for long periods.
Keeping the connection may cause electric shock, current leakage, or fire due to the deterioration of insulation.
If the unit is to be stored unused in an unsupervised area for an extended period, ensure that children do not have access and that doors cannot be closed completely.
The disposal of the unit should be accomplished by appropriate personnel.
Remove doors to prevent accidents such as suffocation.
Do not put the packing plastic bag within reach of children as suffocation may result.
Use the reagent specified by our company for H
2
O
2
decontamination . Using a different H
2
O
2 solution may result in explosion or damage to the incubator.
When performing H
2
O
2
decontamination, securely close the internal and external doors . Failure to do so may be harmful to health due to leakage of H
2
O
2
gas.
During H
2
O
2
decontamination , plug the access hole with the silicon cap that is provided.
Failure to do so may be harmful to health due to leakage of H
2
O
2
gas.
7
PRECAUTIONS FOR SAFE OPERATION
WARNING
Always use the removable power supply cord that is provided. Other power supply cord may cause electric shock or fire.
Do not position this unit and the other unit so that it is difficult to operate the disconnection of the power supply plug.
Failure to disconnect the power supply plug may cause fire if there is something wrong with the unit.
CAUTION
This unit must be plugged into a dedicated circuit protected by branch circuit breaker.
Use a dedicated power source as indicated on the rating label attached to the unit. A multiple-tap may cause fire resulting from abnormal heating.
Never store corrosive substances such as acid or alkali in this unit if the container cannot be sealed. These may cause corrosion of inner components or electric parts.
Check the setting when starting up of operation after power failure or turning off of power switch.
The stored items may be damaged due to the change of setting.
Be careful not to tip over the unit during movement to prevent damage or injury.
Prepare a safety check sheet (copy the last page) when you request any repair or maintenance for the safety of service personnel.
Wear rubber gloves when handling the H
2
O
2
reagent . Direct contact with the H
2
O
2
reagent may result in inflammation of the skin.
H
2
O
2
decontamination can be performed only for the chamber and chamber attachments with standard specifications, and not for any other objects.
Perform H
2
O
2
decontamination with the chamber attachments arranged as specified by our company.
Arranging them in a different way may result in insufficient decontamination.
After H
2
O
2
decontamination has been completed, wear rubber gloves and use a non-woven cloth to wipe off the residual H
2
O
2
fluid from the bottom of the chamber, any objects that were decontaminated, and the bottoms of ducts.
8
LABELS ON INCUBATOR
Warning and caution labels are attached to the incubator. The following table describes the labels.
This label is attached to covers that access high-voltage electrical components to prevent electric shock. Only a qualified engineer or service personnel should be allowed to open these covers.
This symbol indicates an ultraviolet light (UV) caution.
This symbol indicates that caution is required. Refer to product documentation for details.
This symbol indicates a hot surface.
This symbol indicates an earth.
This symbol means “ON” for a power switch.
This symbol means “OFF” for a power switch.
9
ENVIRONMENTAL CONDITIONS
This equipment is designed to be safe at least under the following conditions (based on the IEC 61010-1):
■ Indoor use;
■ Altitude up to 2000 m;
■ Temperature 5 o C to 40 o C
■ Maximum relative humidity 80 % for temperature up to 31 o C decreasing linearly to 50 % relative humidity at 40 o C;
■ Mains supply voltage fluctuations up to ±10 % of the nominal voltage;
■ Transient overvoltages up to the levels of OVERVOLTAGE CATEGORY II;
■ Temporary OVERVOLTAGES occurring on the mains supply;
■ Applicable pollution degree of the intended environment (POLLUTION DEGREE 2 in most cases);
10
INCUBATOR COMPONENTS
Unit
Rear side
Access port cover
Silicon cap
20
21
1 2 3 Tray 4 5
Handles
Leveling feet
19
Leveling
Foot
6
7
8
(When some inner attachments are removed)
13
12* Electric key* 11 10 9
* When an optional electric lock MCO-170EL is installed.
Lower right side
Sample air outlet cap
(When 13 is removed)
Removable power supply cord port
18 17 16 15 14
11
INCUBATOR COMPONENTS
1.
Outer door: The outer door is held to the frame with the magnetic seal. The door heater is installed in the door panel. The door opening is reversible. Contact our sales representative or agent to change the door hinge from left to right or vice versa.
2. Inner door: The inner door is made of tempered glass. However do not subject the glass to excessive impacts.
3. Tray catches: Insert tray to fit the concave portion on chamber.
4. Fan cover: The fan cover serves as the inlet for circulating air. It is removable.
5. Duct: The duct for the path for circulating air. It is removable.
6. Fan (inside the duct): The fan is made from polypropylene resin. It can be disinfected in an autoclave.
7. UV lamp: This UV lamp does not generate ozone. Never look directly at the UV light. Refer to page
57~61 for using. For replacement, contact our sales representative or agent.
8. Humidity control bar: Reduce automatically dew condensation occurred by the effect of outside environment and the frequency of door opening/closing.
The humidity control bar has bactericidal effects by plated surface of it. However, it is recommended to replace the humidity control bar every 5 to 6 years to maintain bactericidal effects. (The duration of bactericidal effects differs depending on the use environment.)
9. Humidifying pan cover: This cover prevents the UV light entering the chamber. Always use it. Using without it may have a bad influence on the chamber temperature distribution and humidity recovery.
10. Humidifying pan: Fill the humidifying pan with sterile distilled water, and set the humidifying pan with the inner side flush against the back. Install the humidifying pan in a longitudinal direction as its shorter side is placed in the back.
11. Door switch: Detects the door opening/closing and stops the fan and electromagnetic valve for CO
2 when the door is open. The UV lamp* 1 is also deactivated by the door opening.
12.
Key hole*: This is the hole to unlock with unlock key while outer door is locked by electric lock.
13. Switch cover: Prevent the accident of gas tube disconnected by the unexpected touch or power off.
14. Power supply cord cover plate: This plate is to prevent the removable power supply cord being come off.
15. Connecting port A for CO
2
gas pipe: Refer to page 21 for gas cylinder connection. Ensure that the gas pressure is set at 0.03 MPa(G)~0.1 MPa(G) (0.3 kgf/cm 2 (G)~1 kgf/cm 2 (G), 4.4 psi(G)~14.5 psi(G)).
Note: When the optional MCO-21GC gas auto changer is installed, both ports A and B are available. Refer to page 75 for gas auto changer.
16. Glow starter: The glow is started for the UV lamp.
17. Power switch: The power switch of this incubator (ON-“I”, OFF-“O”). It also functions as an overcurrent breaker.
18. Sample air outlet: The sample air outlet also functions as an internal gas outlet. Normally, cover this outlet with the sample air outlet cap.
19. USB port: Insert USB memory to export operations and alarms log. Refer to page 42~53.
Note: It is impossible to use USB memory which is required password input.
20. Access port: Place the silicon caps on both outside and inside of the port when the port is not being used.
21. Remote alarm terminals: This terminal informs the alarm to remote location by connecting to external alarm unit. Refer to page 15.
12
LCD touch panel
1
3
The following display (called the Top screen) will appear when the power switch is turned ON.
Note: It takes approximately 20 seconds until Top screen is displayed. During warming-up, “Status: Gas sensor initializing” is displayed in the Message display field ( 13 ), and “--.-” is displayed in the Present CO
2 density display field ( 4 ).
2 6 7 8
1. Present temperature display field
The current chamber temperature is displayed.
2. Set temperature value display field
The set value of chamber temperature is displayed. Default setting: 37 o C.
6. CO
2
gas injection indicator
This lamp lights when CO
2
gas is being injected.
7. CO
2
gas supply line indicator A and B* 1
Current supplying CO
2
gas supply line
(connecting port for CO
2
gas pipe) is displayed. The connecting port A/B for gas 3. Heating indicator
This lamp lights when the heater is energized.
4. Present CO
5. Set CO
2
2
density display field
The current chamber CO
2
density is displayed.
Nothing is displayed when CO
2 density is set 0 %.
density value display field pipe that is currently supplying CO
2
is displayed in reverse video and blinks.
8. CO
2
gas supply line select key* 1
This is a key to select CO
2
gas supply line A or B (Connecting port A or B for CO
2
gas pipe). When an optional gas auto charger
MCO-21GC is installed, CO
2 gas supply line The set value of the chamber CO
2
density is displayed. Default setting: 0 %. A/B changes over automatically when CO
2 gas cylinder is empty. Changeover is also workable by pressing this key.
*1: Only when an optional component MCO-21GC (Gas auto charger) is installed, this key is workable. They are not displayed when the MCO-21GC is not installed.
5
4
13
INCUBATOR COMPONENTS
13
10 11 9 12
14
18 17 16 15
9. Present date/time display field 13. Message display field
Normally, this indicator shows date and time.
The date and time is simply set when the incubator is shipped from the factory. Refer to page 54 for details.
10. Over heat display
High limit temperature alarm is activated:
“Over Heat” is displayed alternately in normal characters and reverse video.
11. UV lamp condition display
UV lamp ON: “UV : On” is displayed.
UV lamp OFF: “UV : Off” is displayed.
12. Outer door (opening/closing) display
Open: “Door : Open” is displayed alternately
Alarms, errors or messages are displayed when fault occurs. Refer to page 83~85.
Note: When there are a number of alarms/errors, the display shows the message.
For example, if 2 alarms/errors occur in total, the display shows “1/2”.
14. Message select key
When there are a number of alarm/errors, the message on the screen is changeable.
15. Menu key
Press this key to lead the Menu screen. It is possible to set various setting on the Menu screen. Refer to page 30. in normal characters and reverse video.
Close: “Door : Closed” is displayed.
Locked: “Door : Locked” is displayed.* 2
*2: Auto lock function by electric lock is workable when an optional electric lock MCO-170EL is installed. If not, “Door : Locked” or Unlock key are not displayed.
14
16. H
2
O
2
key* 3
This key is to run H to page 62 to 67.
17. Unlock key* 2
2
O
2 decontamination. Refer
Press this key is to unlock the outer door when it is auto-locked by electric lock. Refer to page
73. When the auto lock function is OFF, this key is not displayed.
18. Buzzer key
Press this key to silence the buzzer. However, when the ring back is ON, the buzzer will sound again when the ring back passed and the alarm state still continues. Refer to page
40~41 and 83~85.
Note: It is not possible to silence the buzzer for the high limit temperature alarm.
*3: The H
2
O
2 decontamination function is workable when all H
2
O
2
generator MCO-HP, H
2
O
2
decon board
MCO-170HB and electric lock MCO-170EL are installed in the MCO-230AICUVL. If not, the H
2
O
2 key is not displayed on the LCD touch panel.
Remote alarm terminal
The alarm of this product can be informed at a remote location from this product by connecting the external alarm unit to the remote alarm terminals. For the type and behavior of remote alarm output, refer to page
83 to 85.
The terminal of the remote alarm is installed at the rear upper right of the unit (See the figure on the point). The alarm is outputted from this terminal.
Contact capacity is DC 30 V, 2 A.
When Buzzer key is pressed, the behavior of the remote alarm is showed in Table.1.
Note: In the door alarm, the remote alarm does not work. Refer to page 83 to 85.
Table 1 The behavior of the remote alarm when pressing the Buzzer key
Remote alarm terminal
Remote Alarm setting
(Refer to page 39~41)
Connecting terminal
Normal condition
Abnormal condition
(Including in the cases of power outage and of where the power supply plug is pulled out.)
When pressing the buzzer key
ON:
Non-interlock with Buzzer key
OFF:
Interlock with Buzzer key
COM.-N.C.
COM.-N.O.
COM.-N.C.
COM.-N.O.
Close
Open
Close
Open
Open
Close
Open
Close
Open (Maintain in abnormality)*
Close (Maintain in abnormality)*
Close (Return to normal)
Open (Return to normal)
*In case of Err01 (CO
2 gas cylinder empty), Err11, 12(CO
2 sensor error), the condition returns to normal.
15
INSTALLATION
Installation site
For correct operation of the incubator, install it in a location with the following conditions.
WARNING
When using CO
2
gas for control, make sure that there is an adequate ventilation . Using CO
2
gas in a small room without adequate ventilation may cause gas poisoning or oxygen deprivation. In addition, when opening the incubator doors, do not directly inhale the air in the chamber.
Si l’appareil est utilisé dans un evdroit restreint, le niveau de la densite CO
2
de l’air peut s’élever et peut
être nocif aux humains. Evitez d’aspirer l’air provenant de l’inérieur de l’appareil quand vous ouverz la porte.
Normal air environment
Install the incubator in an environment with normal air.
Do not expose to direct sunlight
Do not install the incubator in a location where it will be exposed to direct sunlight. If the incubator is operated in direct sunlight, performance will be adversely affected.
Separate from heat sources
Do not install the incubator near significant heat sources, such as heaters, boilers, ovens, or autoclaves.
Heat will adversely affect the performance of the incubator.
Ambient temperature at least 5 C lower than set temperature
The control temperature of the incubator is at least 5 C higher than the ambient temperature. For example, if the chamber is controlled at 37 C, the ambient temperature must be 32 C or less. Do not allow the ambient temperature to become too high.
Strong and level floor
Select a site with a strong and level floor. If the floor is uneven or the installation is not level, the incubator will be unstable and this may cause accident or injury. To avoid vibration and noise, always make sure that the installation is stable. An unstable surface may result in vibration or noise.
WARNING
Install the incubator at a location that can support the weight.
If the floor is not strong enough or if the installation is insufficient, the incubator may fall over and cause injury.
Always make sure that the floor is strong, even, and level, and that the incubator will not tip over .
An insufficient installation may result in injury due to water leakage or the incubator falling over.
Separate from vibration products
Do not install the incubator near vibration products. Vibration may cause culture failure.
16
Low humidity
Select a site with a relative humidity of 80 %R.H. or lower. Using the incubator in high humidity may result in current leakage or electric shock.
WARNING
Do not use the incubator outdoors.
If the incubator is exposed to rain water, it may result in current leakage or electric shock.
Never install the incubator in a moist location, such as near a sink or water line, or where it is likely to be exposed to water.
In addition, do not install it near water or steam pipes . Moisture can cause the insulation to deteriorate, which may result in current leakage or electric shock.
No inflammable or corrosive gas
Never install the incubator in a location where it will be exposed to inflammable or corrosive gas. Doing so may result in explosion or fire. In addition, insulation may deteriorate due to corrosion of protective casing, resulting in current leakage or electric shock.
No falling objects
Do not install the incubator in a location where there is the possibility of objects falling from above. Doing so may result in damage or accident.
17
INSTALLATION
Installation
1. Remove the packing tape and clean up.
Remove all the tapes that are securing the doors and the inner attachments. Open the doors for ventilation.
If the outer panels are dirty, wet a cloth with a diluted neutral detergent and wipe them. (Undiluted detergent can damage the plastic components. For the dilution, refer to the instruction of the detergent.)
Wipe off the residual detergent with a wet cloth and then wipe off any moisture.
Note: Remove the cable tie banding the power supply cord. Prolonged banding may cause the corrosion of the cord coating.
WARNING
Do not leave the plastic wrapping bags within reach of children.
If the bag is placed over a child’s head, it can block the mouth and nose and cause suffocation.
2.
Set the humidifying pan and humidifying pan cover (Fig. 1).
3.
Set 4 trays (Fig. 2).
Humidifying pan cover
Humidifying pan
Fig. 1
4 Trays
Fig. 2
18
4. Adjust the leveling feet.
Adjust the leveling feet by turning them counterclockwise to level the incubator (Fig. 3).
Note: Incubating on a leaning tray may have a bad influence on the cultivation.
Shrink
Leveling feet Stretch
Fig. 3
5. Ground the incubator.
Ground the incubator during installation to prevent electric shock in case the insulation is not sufficient. If there is no ground wire at the location, consult with qualified service personnel.
When a ground must be installed
If a grounded 3-pole outlet is not available, then a ground must be installed. Consult with qualified service personnel.
WARNING
To prevent electric shock, always ground the incubator.
If grounding is not possible, then have a ground installed by qualified personnel. If the incubator is not grounded, it may result in electric shock.
Never connect the ground wire to a gas pipe, water pipe, lightning rod, or telephone ground wire.
Doing so may cause electric shock.
Installing a ground fault circuit breaker
If using the incubator in the location with moisture or humidity cannot be avoided, then it is recommended that a ground fault circuit breaker be installed in the power supply circuit (i.e., the power supply at the incubator). Have the circuit breaker installed by qualified service personnel.
CAUTION
Do not climb on the incubator or place objects on top of it.
Doing so may damage it or cause it to fall over, resulting in injury.
19
INSTALLATION
In case of double stack
For stacking the incubators surely, refer to the procedure included with the optional double stacking bracket MCO-170PS or the stacking plate
MCO-230SB.
Note: When stacking incubators, fix two hooks on the rear of the upper incubator to the wall by wire or chain, to prevent falling over (Fig. 4).
Note: When stacking the incubators with our CO
2
Wire
Hooks incubator or O
2
/CO
2
incubator other than this product, use the stacking plate MCO-230SB. Refer to table 11 and 12 on page 91.
Fig. 4
When the incubator is not in use
Empty the water from the humidifying pan and remove moisture from the chamber. Make sure that the chamber is completely dry before closing the doors. Failure to do so may result in damage.
Before moving the incubator
Before moving the incubator, empty the water from the humidifying pan, disconnect the power supply plug from the outlet, and make sure that the cord will not be damaged. Failure to do so may result in electric shock or fire.
20
Connecting CO
2
gas cylinder
WARNING
When connecting a gas cylinder to the incubator, confirm the gas type . Confirm that the connections are secure and that no gas will leak . Be sure to use the specified pressure . Using an incorrect gas or pressure may result in explosion or fire, or in gas poisoning or oxygen deprivation due to gas leak.
Install the incubator in a location with adequate ventilation.
If adequate ventilation cannot be provided, then install an alarm system using CO
2
and O
2 densitometers.
1.
Get a CO
2 gas cylinder ready and install an optional gas regulator MCO-010R.
Note:
· Use a liquefied CO
2
gas cylinder (at least 99.5 % pure). The siphon (dip tube) type cannot be used.
· When MCO-010R is not available, install a gas regulator rated at 25 MPa(G) (250 kgf/cm 2 (G), 3600 psi(G)) for the primary side, and 0.25 MPa(G) (2.5 kgf/cm 2 (G), 36 psi(G)) for the secondary side.
2.
Using a gas tube that is provided, connect the connecting port A for CO
2
gas pipe and the gas regulator of the CO
2
gas cylinder.
Lower right side
For details on installing the optional automatic CO
2 cylinder changeover kit (MCO-21GC), refer to page
75~77.
Connecting port A for CO
2
gas pipe
Note:
· By using 2 tube bands that is provided, connect the gas tube tightly to prevent it from coming off.
· Make sure that the gas tube is not folded.
· If the CO
2
gas is supplied to multiple CO
2
incubators from a single gas cylinder, a CO
2
solid will be formed in the gas regulator. The gas regulator safety valve will operate, and there may an explosive sound.
3.
After connecting the gas tube, make sure that no gas is leaking (ex. by using a gas leak detection spray).
4. Set the CO
2
gas on the secondary side to 0.03 MPa(G)~0.1 MPa(G) (0.3 kgf/cm 2 (G)~1 kgf/cm 2 (G), 4.4 psi(G)~14.5 psi(G)) for gas injection. Recommended pressure: 0.03 MPa(G) (0.3 kgf/cm 2 (G), 4.4 psi(G)).
Note: As the pressure increases, the CO
2
gas density control range will increase. Excessive pressure may cause gas supply lines inside the incubator to come loose, which may result in gas poisoning or oxygen deprivation due to gas leak. If gas lines come loose, the incubator must be repaired.
5.
When there is no CO
2 gas left and the CO
2 gas empty alarm is activated, replace the empty gas cylinder to a new one.
Note: When an optional gas auto changer MCO-21GC is installed, it switches the empty CO
2 gas supply line to the other automatically. Refer to page 76~77.
Note: The gas lines connected to the incubator will degrade over time. If any deterioration or abnormalities are found during inspection, replace the lines immediately.
21
BEFORE COMMENCING OPERATION
Initial cleaning method
Before using the incubator for the first time, clean dirt (tape residue, smear, etc.) from the chamber and the inner attachments thoroughly. To keep the chamber clean is essential to get the proper performance out of the incubator. Use the following steps to clean the incubator properly.
1. Remove the inner attachments, referring to “Removing inner attachments” on page 23.
2. Clean the removed inner attachments, the chamber inside walls and the inner door gaskets with a cloth or sponge soaked in neutral detergent, diluted by 5 % or less. (Undiluted detergent can damage the plastic components. For the dilution, refer to the instruction of the detergent.) (Fig. 1)
CAUTION
Do not use detergents or antiseptic solutions with acid, alkali, or chlorine. Doing so may cause discoloration, corrosion, or rusting.
Be careful to keep the diluted detergent or water out of the temperature sensor, the CO
2
gas injection port, the inner sample air access port, the fan motor shaft bearing, and the inner sample air outlet (Fig.2 ).
Also, do not wash the temperature sensor and the UV lamp using detergent. Doing so may cause failure.
(Fig. 2)
3. Soak a gauze or unwoven cloth in distilled water and wring it tightly, and then wipe off the residual detergent thoroughly.
4. Wash the silicon caps (2 pcs) for the access port and the fan using the above mentioned detergent and rinse them with distilled water, and then autoclave them for sterilization (121 o C, 20 minutes).
5. Wipe up the inside walls and the inner attachments like trays thoroughly with a cloth or unwoven cloth soaked in alcohol for disinfection. Be careful not to leave any residue alcohol.
6. Reinstall the inner attachments correctly and securely, referring to “Installing inner attachments” on page
25.
Silicon cap
Temp. sensor
UV lamp
Humidity control bar
Fig. 1 Fig. 2
22
Removing inner attachments
CAUTION
Wear rubber gloves when performing maintenance on the chamber.
Failure to wear gloves may result in cuts or abrasions from sharp edges or corners.
Be careful not to damage the UV lamp in the duct.
1.
Turn OFF the power to the incubator.
2. Open the outer and inner doors and pull out all the trays (Fig. 1).
3.
Pull out the humidifying pan (Fig. 2).
Tray
Fig. 1
4.
Pull out the humidifying pan cover (Fig. 3).
5.
Pull out the fan cover (Fig. 4).
Humidifying pan
Humidifying pan cover
Fan cover
Fig. 2
Fig. 3
Fig. 4
23
BEFORE COMMENCING OPERATION
6.
Lift the duct and remove it from the pins on the rear side (Fig. 5).
7.
Pull out the fan (Fig. 6).
8.
Remove the silicon caps of each access port from interior (Fig. 7) and exterior (Fig. 8).
Duct
Silicon cap
Silicon cap
24
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Installing inner attachments
To re-install all the attachments, perform the procedure in reverse order from step 8 on page 24.
Note: When installing the fan, insert it to the motor shaft securely. Lightly turn and pull the fan
1.Position the center hole of the fan with the projection of the motor shaft.
And insert it deeply. manually to make sure that it does not touch the rear panel and is installed securely (Fig. 1).
CAUTION
If the fan is not inserted deep enough, the intended velocity performance cannot be achieved, and it may cause culture failure or insufficient decontamination.
Note: To install the duct, confirm 4 pins are securely installed in the 4 holes of the duct. (Fig. 2)
CAUTION
2.Lightly turn the fan manually to make sure that it does not touch the rear panel.
3.Lightly pull the fan manually to make sure that it is installed.
Fig. 1
If the duct is fixed insecurely, the intended velocity performance cannot be achieved, which may cause culture failure or insufficient decontamination.
Note: When installing the fan cover, position the long hole of duct with the projection of fan cover and insert directly (Fig. 3). Same applies for the humidifying pan cover.
The fan cover may lean by strongly pushing the head of it in the back. Make sure that there is no space in the lower fan cover after installing because the leaning fan cover may have a bad influence on the camber temperature distribution.
1.Position and insert.
Fig. 2
2.Confirm the direction of fixing position as shown.
CAUTION
If the fan cover is fixed insecurely, the intended velocity performance cannot be achieved, which may cause culture failure.
Note: Set the tray with only the front edge bent down (Fig. 4).
3.Make sure that there is no space in the lower of fan cover.
Bent down
Fig. 3
Fig. 4
25
BEFORE COMMENCING OPERATION
Filling humidifying pan
Use the following procedure to fill the humidifying pan with water or to replace water in the humidifying pan.
1. Pull out the humidifying pan toward you. (Fig. 1)
2. Dispose of the remaining water in the humidifying pan and clean the humidifying pan with a diluted detergent. Then rinse it thoroughly with distilled water and wipe it with alcohol for disinfection.
3. Wipe all moisture from the bottom of the chamber.
4. Return the humidifying pan to the chamber and pour sterile distilled water (approx. 1.5 L, preheated to 37 C). (Fig. 2)
Fig. 1
Fig. 2
Note:
· Operation with no water for humidifying may increase the chamber temperature than the set temperature temporarily.
· Preheat the water to 37 C to be poured into the humidifying pan. Adding low-temperature water will lower the temperature and humidity in the chamber.
· Install the humidifying pan in a longitudinal direction as its shorter side is placed in the back.
· Refill the humidifying pan with water early when the volume of water is decreased.
· Mixing any reagent in the water for humidifying may have a bad influence on the cultivation. Especially when using the UV lamp, do not use any reagent. Because the UV light may deteriorate the reagent mixed with the water for humidifying.
5. Set the humidifying pan with the inner side flush against the back, and close the inner door and the outer door.
Note: Set the humidifying pan with the inner side flush against the back. The humidity control bar in the duct keeps at low temperatures and inner moisture is recondensed. Slide the humidifying pan down right under the humidity control bar, otherwise the recondensed water drops will directly fall to the chamber bottom and will pool in the chamber bottom.
When the pooled water evaporates, it may leave a white mark on the chamber bottom. This is not a malfunction. Wipe it off with alcohol-soaked gauze or unwoven cloth. When the mark cannot be removed, scrub the mark off with using a cream cleanser.
26
FOR BETTER CULTIVATION
Precautions for cultures
Leave space between culture containers.
Always leave space for ventilation between culture containers (Petri dishes, flasks, etc.). Inadequate spacing may result in uneven temperature distribution and CO
2
gas density.
Do not place harmful materials in the chamber.
Never place samples that release acidic, alkali, or corrosive gas in the chamber. Doing so may cause damage resulting from discoloration or corrosion.
Close the inner door.
Always close the inner door before closing the outer door. Failure to close the inner door will adversely affect performance even if the outer door is closed.
Open and close the doors gently.
Always open and close the doors gently. Closing the doors forcefully may cause spillage of the culture medium, incomplete closing, or damage to the gasket. Before opening the inner door, check through the glass to confirm that the UV lamp is OFF.
Be careful when closing the outer door.
Use the handle when closing the outer door. Holding the door in other places may cause injury by getting fingers caught in the door. Do not lean on the outer door. Doing so may result in injury from the outer door coming loose or the incubator falling over, or it may cause current leakage or electric shock.
Be careful of the inside of the outer door.
The inside of the outer door may become hot.
Avoid using excessive force on the inner door.
Do not put your hand on the glass, poke it with sharp objects, or apply strong force. Doing so may result in injury from breaking the glass.
Check the cause of any alarm buzzer.
If an alarm buzzer sounds while the incubator is in use, immediately check the cause of the alarm. For details on what may cause an alarm buzzer to sound, refer to page 83~85.
27
FOR BETTER CULTIVATION
Preventing contamination
To prevent contamination of the chamber, select a suitable installation site.
Avoid locations with high temperatures or humidity.
Avoid locations with high temperatures or humidity, because of a greater presence of microorganisms in the air.
Avoid locations with passers-by or drafts.
Avoid locations near doors, air conditioners, fans, etc., where passers-by or drafts can facilitate the entry of microorganisms into the chamber.
If possible, use a cleanroom.
To achieve a better culture, it is recommended that a cleanroom be used if one is available.
Use clean containers.
The greatest cause of contamination is dirty containers for cultures. Be careful not to get containers or trays dirty when taking them in and out.
Keep the chamber clean.
Wipe off any fingerprints. If water spills from the humidifying pan, or if the doors are left open for a long time, condensation may form on the inside of the doors. If that occurs, wipe off the condensation with a dry sterile gauze. In particular, clean and disinfect the chamber if the culture medium is spilled. For details, refer to “ROUTINE MAINTENANCE” on page 82.
Use sterile distilled water in the humidifying pan.
Always use sterile distilled water in the humidifying pan. Do not use ultrapure water, because it may cause red rust-like particles in the humidifying pan. Clean the humidifying pan once a month. In some cases, an antibacterial ingredient may precipitate in the water for humidifying. This is not a malfunction.
Keep the incubator out of direct airflows from air conditioners or fans.
Cool airflow from an air conditioner may cause condensation and lead to possible contamination.
28
CORRECT OPERATION
Use the following procedure to start trial operation or actual operation of the incubator.
1. Install the incubator correctly, referring to “INSTALLATION” on page 16 to 21.
2. Remove the packing materials from the chamber and inner attachments. Clean and disinfect the chamber and all the inner attachments, referring to “ROUTINE MAINTENANCE” on page 82.
3. Add approximately 1.5 L of sterile distilled water to the humidifying pan (Refer to page 26).
4.
Connect the removable power supply cord that is provided, to the port on the lower rear side.
5.
Connect the removable power supply cord to the outlet.
6.
Turn ON the power switch on the lower right side of the incubator.
7.
Set the frequency of a power supply on the LCD touch panel (Refer to page 58~59).
WARNING
Always use the removable power supply cord that is provided. Other power supply cord may cause electric shock or fire.
The provided removable power supply cord is only for this product.
Never use it for any other products.
When the incubator is not in use
Empty the water out of the humidifying pan and remove moisture from the chamber. Make sure that the chamber is completely dry before closing the doors. Failure to do so may result in damage.
Before moving the incubator
Before moving the incubator, empty the water out of the humidifying pan, disconnect the power supply plug from the outlet, and make sure that the cord will not be damaged. Failure to do so may result in electric shock or fire.
29
BASIC OPERATION ON LCD TOUCH PANEL
● Operation from Menu key
■ Menu screen Page
♦ Set → ■ Stand-by Setting screen (Setting) Temp.,
2
density, high limit temp. alarm 33 ~ 34
♦ Log
♦ Lock → ■ Lock screen
♦ Auto-Lock →
■ Auto-Lock screen* 1
♦ KeyLock →
■ Key Lock screen
♦ Auto-Lock
User
■ Auto-Lock
User screen
♦ Tools#1 → ■ Tools#1 screen
♦ Temp./ Gas Calibration
♦ Alarm Setting #1
♦ Alarm Setting #2
(Setting) Brightness, sleep ON/OFF etc
(Setting) Don’t push (Only for MTR-5000 user)
→ ■ Log screen
♦ Chart → ■ Chart screen
♦ Actual Temp. (Display) Chamber temp. log graph (can be exported) 43 ~ 45
♦ Actual CO
2
Level (Display) CO
2
density log graph (can be exported) 43 ~ 45
♦ Door Opening (Display)
Outer door opening log graph
(can be exported)
♦ Data
Export
→ ■ Export screen
♦ Setting
♦ Alarm
♦ Alarm Export
♦ Actual Temp. (Export) Chamber temperature log
♦ Actual CO
2
Level (Export) CO
2
density log
♦ Door Opening (Export) Outer door opening log
(Setting) Log interval, Unique ID
(Display) Alarm log (can be exported)
(Export) Alarm log
♦ UV Setting
♦ STD Gas Setting* 2
♦ STD Gas Calibration* 2
♦ Tools#2 → ■ Tools#2 screen
♦ Date & Time
♦ Brightness/Sleep
♦ DAQ Setting
(Setting) Auto-lock ON/OFF, User-ID ON/OFF
(Setting) Key lock ON/OFF, password
43 ~ 45
46 ~ 48
46 ~ 48
46 ~ 48
42 ~ 43
49 ~ 50
51 ~ 53
70 ~ 74
35 ~ 38
68 ~ 70
Don’t push (See Next page)
(Setting) Temp. alarm, CO
2
density alarm, alarm delay 39
(Setting) Door alarm delay, ring back, remote alarm 39 ~ 41
(Setting) UV timer, power supply frequency
(Operate) Lighting UV lamp for 24 hours
(Setting) CO
2
density of standard gas
58 ~ 59
60 ~ 61
79 ~ 80
(Operate) Calibration of CO
2
density by STD gas 80 ~ 81
54
55 ~ 56
30
*1: When an optional electric lock MCO-170EL is installed.
*2: When an optional STD gas auto calibration kit MCO-SG is installed.
Note: Service key is not available. (Qualified engineer only)
Note: On the Tools #1 screen, by mistakenly pressing Temp./Gas Calibration key, Temp./Gas
Calibration screen is displayed.
When this screen is displayed, press Back key to return to the Tools #1 screen, or press Top key to return to the Top screen.
When these settings on this screen are changed, inaccurate temperature or CO
2
density may be displayed.
● Operation from other than Menu key
♦
CO
2
gas supply line select key: (Operate) Manual changeover between CO
2
gas supply line A and B (Page 78)
♦
Message select key:
(Operate) Changing some messages
♦
H
2
O
2
key* 3 : (Operate) H
2
O
2
decontamination (Page 62~67)
♦
Unlock key* 4 : (Operate) Unlock the outer door. (Page 68~72)
♦
Buzzer key: (Operate) Silencing the buzzer (Alarm is not canceled except for some alarms; page 84)
*3: When H
2
O
2
generator MCO-HP, H
2
O
2
decon board MCO-170HB and electric lock MCO-170EL are installed in the MCO-230AICUVL.
*4: When an optional electric lock MCO-170EL is installed, the auto lock function is ON.
31
BASIC PARAMETERS
Numerical input to input window
On each screen in the LCD touch panel, it may be necessary to input numerical values on the numerical input box.
1.
By pressing numerical input box, input window is displayed.
Numerical input box
2.
Press Numerical key or Up/Down key to input numerical value, and press OK key.
● Key description
· Numerical key (0~9):
Input numerical values.
· Up/Down key ( ▲ / ▼ ):
Increases or decreases the numerical value displayed in the numerical input box.
· Clear key:
Deletes the numerical value displayed on the numerical input box.
· Cancel key:
Input window
Stops inputting on the numerical input box and closes the input window.
Note: While the input window is open, it is not possible to operate Top key and Back key.
Note: Up/Down key may not be displayed.
32
Setting temperature, CO
2
density and high limit temperature alarm
Set the chamber temperature, the CO
2
density and the temperature of the high limit temperature alarm for normal operation according to the following procedure. The incubator automatically starts operation using these settings after power-on.
1.
Press Menu key to lead the Menu screen.
2.
Press Set key to lead the Stand-by Setting screen.
33
BASIC PARAMETERS
3.
Input each parameter. Press Apply key to save the input value. The display returns to the Menu screen.
● Each parameter setting
· Temperature: Set value of chamber temperature.
Settable range: 0.0
o C~50.0 o C, factory setting: 37.0 o C.
· CO
2
Density: Set value of chamber CO
2
density. By holding CO
2
Density slide key and sliding it to the right, CO
2
density control turns to ON. Settable range: 0.0 %~20.0 %, factory setting: 0.0 % (OFF).
· High Limit: The high limit temperature alarm is different from the Automatic set temperature alarm (page
39), and it is independent temperature alarm. In case the chamber temperature exceeds the temperature of the high limit temperature alarm, this alarm is activated.
Settable range: 20.0
o C~53.0 o C, factory setting: 53.0 o C.
Refer to page 83~85 for detail of each alarm.
Note:
· When changing the set temperature from less than 45.0 o C to 45.0 o C or higher, the incubator readjusts the CO
2
sensor. During readjusting, “Status: Gas sensor initializing” is displayed in the Message display field, and “--.-” is displayed in the Present CO
2
density display field. After 15 minutes in the shortest, the incubator returns to the normal operation.
· When operating the incubator for the first time or after not using it for an extended period of time, operate it for at least about 4 hours until the chamber temperature and the CO
2
sensor are stable after setting the chamber temperature to the desired temperature and setting the CO
2
density to 0 %. Then change the setting to the desired CO
2
density.
· Set the temperature of the high limit temperature alarm after the chamber temperature is stable at the set value.
· Set the high limit temperature alarm to at least 1 o C higher than the chamber set temperature.
4.
On the Menu screen, press Back key to return to the Top screen.
34
Setting key lock
1.
Press Menu key to lead the Menu screen.
2.
Press Lock key to lead the Lock screen.
3.
Press KeyLock key to lead the Key Lock screen.
35
BASIC PARAMETERS
4.
On the Key Lock screen, it is possible to set each setting of key lock. Press Apply key to change key lock ON and to save the password. The display returns to the Lock screen.
● Each setting of key lock
· Key Lock: By holding Key Lock slide key and sliding it to the right, Key Lock turns to ON.
· Password: The number (Max. 6-digit) inputted here are registered the release password of Key Lock.
· Confirm Password:
To prevent erroneous input, input the same password as Password input box. When inputting different password, Notice dialog box is displayed.
Press OK key and input the correct password.
Note: To prevent abuse of the release password of Keylock, manage properly by limited administrators.
5.
On the Lock screen, press Top key to return to the Top screen.
36
● Operation for Keylock-ON
· When pressing any key except the CO
2
gas supply line select key, Buzzer key and Unlock key,
Password input box is displayed, and input of the release password of Key Lock is required.
· When the inputted password is incorrect, Notice dialog box is displayed. Press OK key, and then input the correct password.
Removing key lock
1.
By pressing Menu key, the Password input window is displayed.
2.
On Password input box, input the set release password of Key Lock, and press OK key to lead the Menu screen.
37
BASIC PARAMETERS
3.
Press Lock key to lead the Lock screen.
4.
Press KeyLock key to lead the Key Lock screen.
5. On the Key Lock screen, by holding Key Lock slide key and sliding to the left, change to OFF.
Press Apply key to turn the key lock OFF. The display returns to the Lock screen.
Note: The release password of key Lock is deleted.
6.
On the Lock screen, press Top key to return to the Top screen.
38
ALARM PARAMETERS
1.
Press Menu key to lead the Menu screen.
2.
Press Tools #1 key to lead the Tools #1 screen.
3.
On the Tools #1 screen,
· Press Alarm Setting #1 key to lead the Alarm
Setting #1 screen, it is possible to set automatic set temperature alarm, automatic set CO
2
density alarm and alarm delay of each alarm (go to procedure 4 ).
· Press Alarm Setting #2 key to lead the Alarm
Setting #2 screen, it is possible to set door alarm delay, ring back and remote alarm (go to procedure
5 ).
4.
On the Alarm Setting #1 screen, input each parameter. Press Apply key to save the input value.
The display returns to the Tools #1 screen.
● Each parameter setting
· Temp. Alarm:
When the chamber temperature exceeds the scope, the set temperature ± the set value of Automatic set temperature alarm, the alarm is activated. Settable range: ±1.0 o C~±5.0 o C, factory setting: ±1.0 o C.
39
ALARM PARAMETERS
· Automatic set temperature alarm delay (Temp. Alarm Delay):
The function is that when the incubator is in the alarm state of automatic set temperature, the alarm buzzer will sound after the automatic set temperature alarm delay set time passed.
Settable range: 0 minute~15 minutes, factory setting: 15 minutes.
Note: When the incubator is recovered from the alarm state of automatic set temperature within the automatic set temperature alarm delay time, the buzzer doesn’t sound after the elapse of the automatic set temperature alarm delay.
· CO
2
Alarm:
When the chamber CO
2
density exceeds the scope, the set CO
2
density ± the set value of Automatic set
CO
2
density alarm, the alarm is activated. Settable range: ±0.5 %~±5.0 %, factory setting: ±1.0 %.
· Automatic set CO
2
density alarm delay (CO
2
Alarm Delay):
The function is that when the incubator is in the alarm state of automatic set CO
2
density, the alarm buzzer will sound after the automatic set CO
2
density alarm delay set time passed.
Settable range: 0 minute~15 minutes, factory setting: 15 minutes.
Note: When the incubator is recovered from the alarm state of automatic set CO
2
density within the automatic set CO
2
density alarm delay time, the buzzer doesn’t sound after the elapse of the automatic set
CO
2
density alarm delay.
5.
On the Alarm Setting #2 screen, it is possible to set each alarm. Press Apply key to save the input value and setup. The display returns to the Tools #1 screen.
● Each setting
· Door Delay:
The function is that when the incubator is in the alarm state of door, the alarm buzzer will sound after the alarm delay set time passed. Settable range: 1 minute~30 minutes, factory setting: 2 minutes.
Note: When the incubator is recovered from the alarm state within the door alarm delay time, the buzzer doesn’t sound after the elapse of the door alarm delay.
· Ring Back:
The function is that the alarm buzzer sounds again when the alarm state still continues after the alarm delay set time passed even though the alarm buzzer was stopped by pressing Buzzer key. By holding and sliding Ring Back slide key to the right, the Ring Back is turned to ON. Settable range: 1 minute~99 minutes, factory setting: 30 minutes.
Note: At Err01 (CO
2
gas cylinder empty), Err11•12 (CO
2
sensor error), Err18 (UV lamp failure) and Door alarm, the alarm is not re-activated because the alarm itself is deactivated by pressing Buzzer key (refer to page 83 and 84).
· Remote Alarm:
The function is that the remote alarm is continued even though the buzzer is stopped by pressing Buzzer key. By holding and sliding Remote Alarm slide key to the right, the Ring Back is turned to ON (not in conjunction with Buzzer key). Factory setting: ON.
6.
(From procedure 4 and 5 ) Press Top key to return to the Top screen.
40
● At the alarm state
· While the incubator’s alarm is being activated and the buzzer is being sounding, the buzzer is silenced by pressing Buzzer key. For the behavior at the time of pressing Buzzer key and the re-activation of alarm, under each setting condition, refer to Table 5-7 on page 84.
Resolve the cause of the alarm in reference to page 83-85 because the alarm itself is not deactivated by pressing Buzzer key except for some alarms.
Note: The buzzer for the high limit temperature alarm can’t be silenced.
41
OPERATION/ALARM LOG
Setting log interval
The incubator is equipped with a function of saving operation log data (chamber temperature, CO
2
density and open/close state of outer door). Use the following procedure to set the log interval (interval of acquiring the operation log).
1.
Press Menu key to lead the Menu screen.
2.
Press Log key to lead the Log screen.
3.
Press Setting key to lead the Setting screen.
42
4.
On the Setting screen, input Log Interval. Press
Apply key to save the input value. The display returns to the Log screen.
Settable range: 2 minutes~30 minutes.
Factory setting: 6 minutes.
Note: It is possible to register 8-digit alphanumeric characters as the Unique ID. Refer to page 48.
Note: Relation between log interval and the estimated amount of data that can be saved
Log interval=2 minutes: Approx. 46 days
Log interval=6 minutes: Approx. 135 days
Log interval=30 minutes: Approx. 664 days
When saving data more than the above, and the data is overwritten and the old data is delated.
5.
Press Top key to return to the Top screen.
Displaying operation log
Operation log saved in the incubator can be displayed graphically on the LCD touch panel.
1.
Press Menu key to lead the Menu screen.
2.
Press Log key to lead the Log screen.
43
OPERATION/ALARM LOG
3.
Press Chart key to lead the Chart screen.
4.
On the Chart screen, input the date (year / month
/ day) of the operation log you want to display graphically.
5.
On the Chart screen, by pressing Show key after pressing the item you want to display graphically, the graph of each operation log is displayed.
· Actual Temp.:
Chamber temperature log graph
(Go to procedure 6 )
· Actual CO
2
Level:
CO
2
density log graph
(Go to procedure 7 )
· Door Opening:
Open/close state of outer door log graph
(Go to procedure 8 )
44
6.
Actual Temp. log graph is displayed.
· Press Back key to return to the Chart screen.
· Press Top key to return to the Top screen.
7.
Actual CO
2
Level log graph is displayed.
· Press Back key to return to the Chart screen.
· Press Top key to return to the Top screen.
8.
Door Opening log graph is displayed.
· Press Back key to return to the Chart screen.
· Press Top key to return to the Top screen.
To previous day
To previous day
To previous day
To next day
To Actual CO
To next day
2
To Actual Temp. log
To Door Opening log
To Actual CO
To next day
Level log
2
Level log
Note: The error of about 1 minute may be observed during a month. Refer to page 54 for the procedure of setting time.
45
OPERATION/ALARM LOG
Exporting operation log
Operation log data saved in the incubator can be exported in CSV format to the USB memory inserted into the USB port.
1.
Insert the USB memory into the USB port.
Note: It is not possible to use a USB memory with security functions that requires entering password.
2.
Press Menu key to lead the Menu screen.
3.
Press Log key to lead the Log screen.
4.
Press Data Export key to lead the Export screen.
46
5.
On the Export screen, select the time period you want to export.
· To export the saved operation log data over the entire period, press All radio button.
· To export the operation log data of a specified date, press 1 Day radio button and input the date (year / month / day) of the operation log data you want to export.
Note: The error of about 1 minute may be observed during a month. Refer to page 54 for the procedure of setting time.
6.
On the Export screen, select the type of operation log data you want to export.
· To export all types of operation log data, press All
Ch key.
· To export only operation log data you want to export, select operation log data you want to export, port. and then press Selected Ch key.
· Actual Temp.: Chamber temperature log data
· Actual CO
2
Level: CO
2
density log data
· Door Opening: Open/close state of outer door log data*
* When both of the Auto-lock function and the User-ID mode are ON (refer to page 68~72), inputted
User-IDs for unlocking the outer door are also exported.
Note: When no USB memory is inserted into the
USB port, Notice dialog box is displayed. Press OK key, and then insert a USB memory into the USB
47
OPERATION/ALARM LOG
Note: When the specified operation log data doesn't exist, Notice dialog box is displayed. Press
OK key, and then re-specified according to procedure 4 and 5 .
7.
When the export is complete, Information dialog box is displayed. Press OK key.
Note: Even after the export of operation log data is complete, operation log data saved in the incubator are not deleted.
8.
Remove the USB memory from the USB port.
Note:
· The log folder is created in the USB memory, and the exported file is saved in it in CSV format. The exported file name is in date (8 digits) - type of data format.
(e.g.) When exporting all types of data using All (from Oct. 1st, 2017 to Jan. 1st, 2018):
20171001-20180101_AllCh.csv
20171001-20180101_Door.csv
(e.g.) When exporting Actual Temp. using 1 Day (Jan. 1st, 2018):
20180101_Temp.csv
· On the beginning of the exported file, “MCO-230AIC” is written. However when the Unique ID is registered (refer to page 43), “MCO-230AIC” and Unique ID (8-digit) are written.
(e.g.) When “RoomA001” is set as the Unique ID of MCO-230AICUVL:
MCO-230AIC, RoomA001
9.
Press Top key to return to the Top screen.
48
Displaying alarm log
The incubator is equipped with a function of saving alarm log data (Max. 256 logs). Alarm log saved in the incubator can be displayed graphically on the LCD touch panel.
Note: When saving alarm logs more than 257, the oldest alarm log is deleted, and then overwritten.
1.
Press Menu key to lead the Menu screen.
2.
Press Log key to lead the Log screen.
3.
Press Alarm key to lead the Alarm screen.
49
OPERATION/ALARM LOG
4.
On the Alarm screen, the newest 7 days’ alarm logs (containing that day) are displayed.
Note: When the number of applicable alarm log is 6 or more, by pressing the top ( ▲ ) or the bottom ( ▼ ) log, the log table currently displayed scrolls and hidden alarm logs can be seen.
· Press Back key to return to the Log screen.
· Press Top key to return to the Top screen.
5.
On the Alarm screen, by inputting days into the
Last XX Days input box, alarm logs for specified days (containing that day) are displayed.
Settable range: 1 day~45 days.
Note: The error of about 1 minute may be observed during 1 month. Refer to page 54 for the procedure of setting time.
· Press Back key to return to the Log screen.
· Press Top key to return to the Top screen.
● On the Alarm screen of procedure 4 or 5 , alarm log data can be exported in CSV format to the USB memory inserted into the USB port.
6.
Insert the USB memory into the USB port.
Note: It is not possible to use a USB memory with security functions that requires entering password.
7.
Press Export key.
8.
When the export is complete, Information dialog box is displayed. Press OK key. Refer to page 52 and 52 for the details about abnormal export or exported file name.
9.
Press Top key to return to the Top screen.
50
Exporting alarm log
It is possible to export saved alarm log data to a USB memory inserted in the USB port by CSV format.
1.
Insert a USB memory in the USB port.
Note: It is not possible to use a USB memory with security functions that requires entering password.
2.
Press Menu key to lead the Menu screen.
3.
Press Log key to lead the Log screen.
4.
Press Alarm Export key to lead Alarm Export screen.
51
OPERATION/ALARM LOG
5.
On the Alarm Export screen, select the period to export.
· To export the saved alarm log data over the entire period, press All radio button.
· To export the alarm log data for the specified days
(The newest period containing that day), press Last
XX Days radio button and input days.
Settable range: 1 day~45 days.
Note: The error of about 1 minute may be observed during 1 month. Refer to page 54 for the procedure of setting time.
6.
Press Export key.
Note:
· When USB memory is not inserted in the USB port,
Notice dialog box is displayed. Press OK key and insert an USB memory into the USB port.
· When alarm log data doesn’t exist in the specified days, Notice dialog box is displayed. Press OK key and specify days again as shown in the procedure
5 .
52
7.
Even after completion the export of alarm log data, Information dialog box is displayed. Press OK key.
Note: After completing the export of alarm log data, alarm log data saved at CO
2
incubator is not deleted.
8.
Remove a USB memory from the USB port.
Note: A log folder is created in a USB memory, and an exported data file is saved in the log folder by CSV format.
Exported file name; The first date during exported period (8 digits) + the last date (8 digits) + AlarmLog
Example) When exporting alarm log data for 7 days on January 7, 2018;
20180101-20180107_AlarmLog.csv
9.
Press Top key to return to the Top screen.
53
OTHER PARAMETERS
Setting date and time
1.
Press Menu key to lead the Menu screen.
2.
Press Tools #2 key to lead the Tools #2 screen.
3.
Press Date & Time key to lead the Date & Time screen.
4.
On the Date & Time screen, input the present date and time. Press Apply key to save the input value. The display returns to the Tools #2 screen.
Note:
· 24-hour clock.
· It is recommended to set the time periodically since the error of about 1 minute may be observed during a month.
5.
Press Top key to return to the Top screen.
54
Setting brightness and sleep
1.
Press Menu key to lead the Menu screen.
2.
Press Tools #2 key to lead the Tools #2 screen.
3.
Press Brightness/Sleep key to lead the
Brightness/Sleep screen.
4.
On the Brightness/Sleep screen, each setting of brightness and sleep is available. Press Apply key to save the input value and setup. The display returns to the Tools #2 screen.
55
OTHER PARAMETERS
● Each setting
· Brightness(Active):
Brightness of LCD touch panel of the usual state. Adjust Brightness(Active) slide bar or input set value into the Brightness(Active) input box. Settable range: 50~100, factory setting: 80.
· Sleep:
The function is that the rightness of LCD touch panel is lowered to save electricity, when there is no key operation during set time.
By holding the Sleep slide key and sliding it right, the Sleep function is turned to ON. Input the set value of time to change the Sleep state. Settable range: 1 minute~5 minutes, factory setting: 2 minutes.
Note: It is not possible to operate any key in the Sleep state. By touching the LCD touch panel, the Sleep state is released and the LCD touch panel returns to the usual state. Under this condition, key operations are available.
· Brightness(Sleep):
Brightness of LCD touch panel of the Sleep state. Adjust Brightness(Sleep) slide bar or input set value into the Brightness(Sleep) input box. Settable range: 0~50, factory setting: 20.
5.
Press Top key to return to the Top screen.
56
UV LAMP PARAMETERS
After closing the outer door, UV lamp lights for the preset period*, to disinfect the water in the humidifying pan, and the air circulating in the chamber.
Using UV lamp
1.
Correctly install all of the inner attachments, and place the cultivation samples on the trays.
Note:
· The humidifying pan and humidifying pan cover prevent UV light from leaking. Always use them even when not humidifying.
· Never turn ON the UV lamp when the humidifying pan cover is removed.
· Always use the humidifying pan cover even when using the incubator without turning ON the UV lamp.
Using without humidifying pan cover may have a bad influence on the chamber temperature distribution and humidity recovery.
2.
When closing the outer door, the UV lamp lights for the preset period*.
Note:
· If the outer door is opened while the UV lamp is lit, the lamp will turn OFF. Then, when the door is closed, the lamp will light for the preset period*.
· If only the outer door is repeated opened and closed, it may have a bad influence on the condensation in the chamber and chamber temperature distribution because the UV lamp generates heat for a long time. It may also shorten the service life of the UV lamp.
· The preset period* can be changed when necessary as shown in the page 58~59.
· To check whether the UV lamp is lit, open the outer door and then press the door switch with the inner door close. Visible blue light can be confirmed from the front of the humidifying pan cover.
Note: UV light is harmful to the eyes, so do not light the UV lamp when the inner door is open.
WARNING
Do not look directly at UV light.
UV light is harmful to the eyes.
3.
If the outer door is not opened for at least 12 consecutive hours, the UV lamp lights for the preset period* every 12 hours.
Note: Outer door opening resets the 12-hours-count.
* The set period in UV Timer setting + the period extended by the UV Timer Ext.. Refer to page 59.
The recommended replacement time for the UV lamp (i.e., when the UV output ratio drops to 60 % to
70 % of its initial value) is when the accumulated ON time reaches 5,000 hours. When the accumulated
ON time reaches approximately 5,000 hours, “Warning: UV Bulb Life” is displayed in the message display field. It is recommended that the UV lamp be quickly replaced at this point. Contact our sales representative or agent for information on replacing the UV lamp.
57
UV LAMP PARAMETERS
If the UV lamp burns out, “Err18: UV Lamp Abnormal” is displayed in the message display field. If this occurs, replace the UV lamp. When replacing the UV lamp, replace the glow starter at the same time.
Contact our sales representative or agent for information on replacing the UV lamp.
If the UV lamp burns out (“Err18: UV Lamp Abnormal” is displayed in the message display field), it is not possible to perform H
2
O
2
decontamination. Replace the UV lamp and the glow starter.
Setting UV lamp ON period
Use the following procedure to change the setting of the UV lamp ON period.
1.
Press Menu key to lead the Menu screen.
2.
Press Tools #1 key to lead the Tools #1 screen.
3.
Press UV Setting key to lead the UV Setting screen.
58
4.
On the UV Setting screen, each setting of UV is available. Press Apply key to save the input value and setup. The display returns to the Tools #1 screen.
● Each setting
· UV Timer:
Set value of period to light UV lamp after closing the outer door.
Settable range: 0 minute~30 minutes, factory setting: 10 minutes.
Note:
· It is recommended to set the UV Timer for 10 minutes. The setting for less than 10 minutes may result in insufficient disinfection.
· When the UV timer is set for 0, the UV lamp does not light.
· UV Life:
The total time which UV lamp has turned on is displayed as the percentage to 5,000 hours which are recommendation time to replace. (It is impossible to set).
· UV Timer Ext.:
The more total time which UV lamp has turned on increases, the more UV ray output declines. In order to cover a decline of the UV ray output, the lighting time of UV lamp is automatically extended with an increase of total lighting time of UV lamp. (The set value of UV Timer is not changed).
Extension rate: 0 %~40 % (It is impossible to set), factory setting: 0 %.
Example) UV Timer: 10 minutes, UV Timer Ext.: 40 % → UV lamp lights for 14 minutes.
· Frequency:
Frequency of a power supply which this product is connected to. Press Frequency radio button of 50 Hz or
60 Hz.
5.
Press Top key to return to the Top screen.
59
UV LAMP PARAMETERS
Lighting UV lamp for 24 hours
If the chamber has been contaminated by dirt or by spilling the medium, use the following procedure to decontaminate the chamber by lighting the UV lamp for 24 hours.
1.
Remove all attachments from the chamber, including the trays, the fan cover, the duct, the fan, the humidifying pan, and the humidifying pan cover. Clean all the attachments in an autoclave or with alcohol for disinfection.
2.
Clean and wipe off inside the chamber with alcohol for disinfection.
3.
Set the CO
2 density to 0 %. Refer to page 33~34.
4.
Press Menu key to lead the Menu screen.
5.
Press Tools #1 key to lead the Tools #1 screen.
6.
Press UV Setting key to lead the UV Setting screen.
60
7.
On the UV Setting screen, by holding the UV 24h
Mode slide key and sliding it right, the UV 24h
Mode is turned to ON. Press Apply key to start the
UV 24-hour mode. The display returns to the Tools
#1 screen.
8.
The UV lamp lights continuously for 24 hours. “UV : ON” is displayed on the UV lamp condition display when UV lamp is lighting.
Note:
· The UV 24-hour mode may cause the automatic set temperature alarm because of a rising chamber temperature.
· After procedure 8 , by opening the outer door when UV lamp is lighting, UV lamp is turned OFF and UV
24-hour mode is canceled by opening the outer door. Redo from procedure 4 to start the UV 24-hour mode again.
9.
Press Top key to return to the Top screen.
10.
24 hours after, UV lamp turns OFF automatically. Install all attachments removed in the procedure 1 .
61
H
2
O
2
DECONTAMINATION
When the chamber is contaminated or when cleaning the chamber prior to starting a culture, it is possible to perform H
2
O
2
decontamination.
H
2
O
2
decontamination function is workable when H
2
O
2
generator MCO-HP, H
2
O
2
decon board
MCO-170HB and electric lock MCO-170EL are installed. If not, H
2
O
2
decontamination can not be performed.
WARNING
Use the reagent specified by our company for H
2
O
2
decontamination . Using a different H
2
O
2
solution may cause explosion or damage to the incubator, or insufficient decontamination.
Do not use chemicals other than the H
2
O
2
reagent, such as alcohol.
Doing so may result in damage to the H
2
O
2
vapor generator.
WARNING
When performing H
2
O
2
decontamination, make sure that the outer and inner doors are securely closed . During H
2
O
2
decontamination, plug the access hole with the silicon caps that are provided. Failure to do so may be harmful to health due to leakage of H
2
O
2
gas.
CAUTION
H
2
O
2
decontamination can be performed only for the chamber and inner attachments with standard specifications, and not for any other objects.
CAUTION
Perform H
2
O
2
decontamination with the inner attachments arranged as specified by our company .
Arranging them in a different way may result in insufficient decontamination.
CAUTION
Wear rubber gloves when handling the H
2
O
2
reagent . Direct contact with the H
2
O
2
reagent may result in inflammation of the skin.
CAUTION
After H
2
O
2
decontamination has been completed, residual H
2
O
2
solution will remain on the bottom of the chamber, the H
2
O
2
vapor generator, and the bottom of the duct. Wearing protective glasses and rubber gloves, wipe it off with a non-woven cloth . Failure to do so may result in a deficient culture.
62
H
2
O
2
decontamination
1. Take out all the trays, the fan cover, the duct, the humidifying pan cover and the humidifying pan from the chamber.
2.
Dispose of the water in the humidifying pan, and wipe inner attachments removed from the chamber, with a gauze containing water or alcohol for disinfection.
3.
Wipe the inside walls of the chamber with a gauze containing water or alcohol for disinfection.
4.
Attach the duct and the fan cover.
5.
Pour one bottle of the H
2
O
2
reagent MCO-H2O2 into the H
2
O
2
generator MCO-HP (Fig. 1).
6.
Set the two pins on the H
2
O
2
generator in the 2
Duct lower part
Pour one bottle of the H
2
O
2
reagent holes on the lower left side of the duct (Fig. 1).
Note: Make sure that the H
2
O
2
generator is securely attached. Uncertain attachment may result in insufficient decontamination.
7.
Remove a connector cap from connector on bottom right of the far side of the chamber. Connect the H
2
O
2
generator and connector by a cable that is provided (Fig. 2).
Note: Be sure to keep the connector cap.
H
2
O
2
generator
Front
Fig. 1
Connector
Fig. 2
8.
Insert 4 trays in the 2nd, the 3rd, the 4th and the 6th tray catches from the top of the chamber side.
Note: The trays included as accessory are designed to be appropriate for decontamination. If half tray
(MCO-35ST, optional) or trays for previous models are used, decontamination may not be sufficiently effective.
63
H
2
O
2
DECONTAMINATION
9.
Set the humidifying pan cover, the humidifying pan removed in the procedure 1 (Fig. 3).
Note: H
2
O
2
decontamination can be performed only for the chamber and inner attachments with standard specifications, not for any other objects.
Front
Humidifying pan cover
Humidifying pan
H
2
O
2 generator
Fig. 3
10.
Make sure that the duct, the fan and the H
2
O
2
generator are securely attached (refer to Fig. 1 to 3 on page 88). After that, close the inner door and the outer door.
11.
Press H
2
O
2
key for 3 seconds to open Setting
Position window.
Note:
· When the model does not have H
2
O
2 decontamination function, H
2
O
2
key is not displayed on the Top screen.
· When key lock is ON, Password input window is opened and input of the release password of Key
Lock is required. Refer to page 37.
12.
Finally make sure that the inner attachments and the H
2
O
2
generator are attached correctly. After confirm, press Next key to lead the H
2
O
2
Decontamination screen.
64
13.
On the H
2
O
2
Decontamination Step1 screen, the system check starts automatically. If the system is normal, the display leads the H
2
O
2
Decontamination Step2 screen. When the system is abnormal, refer to Table 8 on page 85.
14.
On the H
2
O
2
Decontamination Step2 screen, press OK key to lead the H
2
O
2
Decontamination
Step3 screen and H
2
O
2
decontamination is started.
H
2
O
2
decontamination is performed automatically from Step3 to Step8 (procedure 15 ).
Note:
· The outer door is locked with electric lock for safety until completion of H
2
O
2
decontamination.
· Buzzer sounds when H
2
O
2
decontamination is completed (About 100 minutes later).
CAUTION
The electric lock will remain locked if power supply is cut off during H
2
O
2
decontamination. After the power supply is recovers, the H
2
O
2
gas resolution process will start execution and finish automatically. Execute the decontamination again because the decontamination is not completed.
WARNING
Do not use the unlock key to unlock the outer door during H
2
O
2
decontamination or during H
2
O
2 gas resolve by UV. Doing so may cause harm to health from H
2
O
2
gas leakage.
● Step3 is the process to warm the chamber temperature to 45 o C.
Note: By pressing Abort key, H
2
O
2
decontamination is stopped in the middle of decontamination and go to Step8 (The End of H
2
O
2
decontamination).
65
H
2
O
2
DECONTAMINATION
● Step4 is the process to decontaminate in the chamber by generating H
2
O
2
vapor from H
2
O
2 generator.
Note: By pressing Abort key, H
2
O
2
decontamination is stopped in the middle of decontamination and go to Step7 (The process to resolve H
2
O
2
vapor by UV lamp). It is not possible to skip Step7.
● Step7 is the process to resolve H
2
O
2
vapor by UV lamp.
Note: Step5 and Step6 do not exist.
15.
When the H
2
O
2
decontamination is completed, the H
2
O
2
Decontamination Step8 screen is displayed.
Open the outer and inner doors. Disconnect connector of the chamber, cable and H
2
O
2 generator, and remove H
2
O
2
generator and cable from the chamber.
Note: When doing the above work, put on protection glasses and rubber gloves.
16.
On the H
2
O
2
Decontamination Step8 screen, press OK key to return to the Top screen.
Note: When H
2
O
2
generator is connected with connector of the chamber by cable, OK key is not workable.
17.
Dilute the remaining H
2
O
2
reagent in the H
2
O
2
generator with a large volume of water and dispose of it.
Rinse and wash the H
2
O
2
generator with distilled water. Then keep the H
2
O
2
generator in a clean environment outside of the chamber.
Note: Do not wash either the inside or outside of the H
2
O
2
generator with alcohol.
66
18. After H
2
O
2
decontamination, surplus H
2
O
2
liquid will remain at the bottom of the chamber and in the bottom part of the H
2
O
2
generator duct. This solution contains H
2
O
2
at a low density, so put on protective glasses and rubber gloves and wipe it up with a non-woven cloth.
19.
Ventilate the chamber sufficiently and place all the attachments back into the chamber.
Note: After H
2
O
2
decontamination, cover the connector on the chamber side with the connector cap deeply. (Fig. 4)
Precautions when handling H
2
O
2
reagent
Connector cap
Fig. 4
Observe the following precautions when handling optional H
2
O
2
reagent MCO-H2O2.
Handling precautions
· Wear protective equipment, such as protective glasses and rubber gloves.
· Do not use fire in the area where the reagent is being handled.
· Do not leave any reagent in the container after it has been used or while it is being used.
· Do not place inflammable or combustible materials near the area where the reagent is handled.
Precautions for storage
· Store in a cool, dark place.
· Always close the container cover securely to prevent impurities from becoming mixed in the reagent.
· Check the container to make sure that there is no damage, corrosion, or cracking.
· Store the container with the inlet facing upwards, and make sure that the container will not tip or be knocked over.
Precautions for disposal
· Dispose according to the rule in your country.
Using unlock key
Unlocking when power is interrupted
When an optional electric lock MCO-170EL is installed, outer door is locked with electric lock under a power outage. To unlock the outer door while the power is interrupted, use the unlock key that is provided.
To re-lock the outer door, turn the unlock key to the lock direction while the outer door is open. After the outer door has been locked condition manually, then close the outer door.
Note: The outer door cannot be locked by using the unlock key while the outer door is closed. Lock the outer door while it is open. Attempting to turn the unlock key while the outer door is closed may damage the electric lock system.
WARNING
Do not use the unlock key to unlock the outer door during H
2
O
2
decontamination or during H
2
O
2 gas resolve by UV. Doing so may cause harm to health from H
2
O
2
gas leakage.
67
ELECTRIC LOCK (OPTION)
Auto lock function is that the outer door is locked automatically when the setting time passed after the door closed. Auto lock function is workable when an optional electric lock MCO-170EL is installed.
The modes of unlocking the outer door are as follows.
· Quick mode: Press the Unlock key.
· User-ID mode: Input the User-ID and release password of Auto-Lock, after pressing the Unlock key.
Setting User-ID
Before turning the User-ID mode to ON, use the following procedure to register a User-ID and a release password of Auto-Lock.
1.
Press Menu key to lead the Menu screen.
2.
Press Lock key to lead the Lock screen.
3.
Press Auto-Lock User key to lead the
Auto-Lock User screen.
68
4.
On the Auto-Lock User screen, it is possible to register a User-ID and its password. Press Apply key to save the User-ID and its password.
● Each setting of Auto-Lock
· User-ID: The alphanumeric characters (Max. 8-digit) inputted here are registered as a new User-ID.
· Password: The number (Max. 6-digit) inputted here are registered a new release password of Auto-Lock of the User-ID.
Note: It is possible to register only a User-ID without registration of a release password of Auto-Lock.
· Confirm Password:
To prevent erroneous input, input the same password as Password input box. When inputting different password, Notice dialog box is displayed.
Press OK key and input the correct password.
Note:
· A release password of Auto-Lock is for unlocking the outer door. It is different from the release password of Key Lock (refer to page 35~37).
· It is possible to input up to 8-digit alphanumeric characters as a User-ID.
· It is possible to input up to 6-digit numbers as a release password of Auto-lock.
· It is possible to register up to 99 User-IDs (and its passwords). When registering the 100th User-ID, notice dialog box is displayed. Press OK key, and then delete a disused User-ID in reference to page
70.
· To prevent abuse of User-IDs and release passwords of Auto-Lock, manage properly by limited administrators.
69
ELECTRIC LOCK (OPTION)
● Changing a registered User-ID’s password
Input the registered User-ID into User-ID input box, and input its new password into Password input box and Confirm Password box. Press Add key to re-write the new password.
● Deleting a registered User-ID
Input the registered User-ID into User-ID input box, and input its registered password into Password input box. Press Delete key to delete the registered User-ID (and its password).
Note: When deleting all registered User-IDs, the User-ID mode is turned to OFF (refer to page 71).
5.
On the Menu screen, press Back key to return to the Top screen.
Setting auto lock
1.
Press Menu key to lead the Menu screen.
2.
Press Lock key to lead the Lock screen.
3.
Press Auto-Lock key to lead the Auto-Lock screen.
70
4.
On the Auto-Lock screen, each setting of auto lock is available. Press Apply key to turn the auto lock ON and save the set value. The display returns to the Lock screen.
● Each setting of auto lock
· Auto-Lock:
Auto lock function is that the outer door is locked automatically when the setting time passed after the door closed. By holding the Auto-lock slide key and sliding it right, the Auto-lock is turned to ON. Settable range:
1 minute~60 minutes, Factory setting: 1 minute.
· User-ID:
Choose the mode of unlocking the outer door between the quick mode or the User-ID mode. By holding the User-ID slide key and sliding it right, the User-ID mode is turned to ON. Factory setting: OFF (quick mode).
Note:
· When no User-ID is registered, notice dialog box is displayed. Press OK key, and then register a
User-ID and its password in reference to page
68~70.
· In the User-ID mode, User-ID which is inputted to unlock the outer door is saved as the open/close state of outer door log data (refer to page 46~47).
· When changing the User-ID mode to OFF, registered User-IDs are not deleted.
· When deleting all registered User-IDs, the User-ID mode is turned to OFF (refer to page 70).
5.
Press Top key to return to the Top screen.
71
ELECTRIC LOCK (OPTION)
● Unlocking the outer door
· In the quick mode, press the Unlock key on the
Top screen to unlock the outer door.
· In the User-ID mode, when pressing Unlock key on the Top screen, User-ID input box is displayed.
Input the User-ID and its release password of
Auto-Lock.
Note: The User-ID which is inputted at this time is saved as the open/close state of outer door log data (refer to page 46~47).
· When the inputted User-ID or its password is incorrect, Notice dialog box is displayed. Press OK key, and then input the correct User-ID or its password.
Note: When the unlocked outer door is closed and the setting time passes, the unlocked outer door is re-locked automatically.
72
Using unlock key
Unlocking when power is interrupted
When an optional electric lock MCO-170EL is installed, outer door is locked with electric lock under a power outage. To unlock the outer door while the power is interrupted, use the unlock key that is provided.
To re-lock the outer door, turn the unlock key to the lock direction while the outer door is open. Close the outer door after the out door is locked manually.
Note: The outer door cannot be locked by using the unlock key while the outer door is closed. Lock the outer door while it is open. Attempting to turn the unlock key while the outer door is closed may damage the electric lock system.
73
ELECTRIC LOCK (OPTION)
Removing auto lock
1.
Press Menu key to lead the Menu screen.
2.
Press Lock key to lead the Lock screen.
3.
Press Auto-Lock key to lead the Auto-Lock screen.
4.
On the Auto-Lock screen, by holding the
Auto-lock slide key and sliding it left, the Auto-lock is turned to OFF. Press Apply key to change
Auto-lock OFF, and the display returns to the Lock screen.
5.
Press Top key to return to the Top screen.
74
GAS AUTO CHANGER (OPTION)
When an optional gas auto changer MCO-21GC is installed, there are two connecting ports for CO
2
gas pipe, A and B. By connecting two CO
2
gas cylinders, this kit switches the CO
2
gas supply line when one of the CO
2
gas cylinders becomes empty.
Connecting CO
2
gas cylinder
1.
Get two CO
2
gas cylinder ready (CO
2
gas cylinder A and B) and install an optional gas regulator
MCO-010R in both of CO
2
gas cylinders.
Note:
· Use a liquefied CO
2
gas cylinder (at least 99.5 % pure). The siphon (dip tube) type cannot be used.
· When MCO-010R is not available, install a gas regulator rated at 25 MPa(G) (250 kgf/cm 2 (G), 3600 psi(G)) for the primary side, and 0.25 MPa(G) (2.5 kgf/cm 2 (G), 36 psi(G)) for the secondary side.
2.
Using a gas tube that is provided, connect the connecting port A for CO
2
gas pipe and the gas regulator of the CO
2
gas cylinder A.
3.
Using a gas tube that is provided, connect the connecting port B for CO
2
gas pipe and the gas regulator of the CO
2
gas cylinder B.
Lower right side
Connecting port B for CO
2
gas pipe
Connecting port A for CO
2
gas pipe
Note: If the CO
2
gas is supplied to multiple CO
2
incubators from a single gas cylinder, a CO
2
solid will be formed in the gas regulator. The gas regulator safety valve will operate, and it may make an explosive sound.
4.
After connecting the gas tube, make sure that no gas is leaking (ex. by using a gas leak detection spray).
5.
Both CO
2
gas cylinder A and B, set the CO
2
gas on the secondary side to 0.03 MPa(G)~0.1 MPa(G) (0.3 kgf/cm 2 (G)~1 kgf/cm 2 (G), 4.4 psi(G)~14.5 psi(G)) for gas injection.
Note: As the pressure increases, the CO
2
gas density control range will increase. Excessive pressure may cause gas supply lines inside the incubator to come loose, which may result in gas poisoning or oxygen deprivation due to the escaping of gas. If gas lines come loose, the incubator must be repaired.
75
GAS AUTO CHANGER (OPTION)
Automatic CO
2
gas supply line changeover
When an optional gas auto changer MCO-21GC is installed, CO
2
gas supply line indicator A•B and CO
2 gas supply line select key are displayed in the Top screen. CO
2
gas supply line indicator A or B being used is lighted.
CO
2
gas supply line indicator A and B CO
2
gas supply line select key
CO
2
gas supply line being used
76
When the CO
2
density level remains unchanged, even though the CO
2
gas valve in the unit is opened, the unit regards the present connecting CO
2
gas cylinder as an empty. The CO
2
gas supply line is changed over automatically. These movements are displayed (Table. 2).
1.
When CO
2
gas is remaining in CO
2
gas cylinder A, the unit operates with CO
2
gas supplied from CO
2 gas cylinder A (Situation 1 on table 2).
2. When CO
2
gas cylinder A is empty, the level of CO
2
density in the unit does not increase because CO
2 gas is not supplied into the unit even though CO
2
gas valve in the unit is open (Situation 2 on table 2).
3.
When the Situation 2 continues for 2 to 3 minutes, CO
2
gas supply line is changed over automatically by regarding CO
2
gas cylinder as an empty. At this time, CO
2
gas empty alarm is activated, the buzzer sounds, and CO
2
gas supply indicator A is displayed in reverse video and blinks (Situation 3 on table 2).
4.
CO
2
gas empty alarm is released by pressing Buzzer key. The reverse video is put the light off (Situation
4 on table 2).
5.
Exchange the empty CO
2
gas cylinder A into a new one immediately after the Situation 4 (Situation 5 on table 2).
6.
When CO
2
gas cylinder B is empty, it changes into CO
2
gas cylinder A.
Table 2 CO
2
gas supply line automatic changeover
(e.g.) When CO
2
gas cylinder A is empty, it changes over CO
2
gas cylinder B.
CO
2
gas
Situation
Supply line Cylinder A Cylinder B
CO
2
gas supply line indicator
1
CO
2
gas is supplying from valve A.
Light on Light off
Message
2
CO
2
density in the chamber is not increased even if
CO
2
gas valve opens.
3
CO
2
gas supply line is changed over B automatically.
Light on Light off
Reverse video and blink
Light on
Err01:
CO
2
Gas Empty
(and buzzer)
4 Pressed Buzzer key. B Empty Remaining Light off Light on
5
Changed empty gas cylinder A into a new one.
Light off Light on
Note:
· When the Buzzer key is not pressed in the Situation 4 and the CO
2
gas cylinder B gets empty without the
CO
2
gas cylinder A being replaced in the Situation 5 , the operation of switch between CO
2
gas supply line
A and B will be repeated. In this case, replace the both CO
2
gas cylinders, A and B, and press the Buzzer key immediately.
· The changeover of CO
2
gas cylinder is judged by an increase of CO
2
density in the chamber. In case that the gas tube is clogged, the gas is leaking, the gas pressure is dropped down, or the level of valve open for
CO
2
gas cylinder is not enough, etc, the changeover of CO
2
gas cylinder may be done even though the
CO
2
gas cylinder being used is not empty.
77
GAS AUTO CHANGER (OPTION)
Manual CO
2
gas supply line changeover
It is possible to change CO
2
gas supply line manually anytime.
Example) Change CO
2
gas supply line A to B.
1.
Press CO
2
gas supply line select key for a few seconds.
2.
CO
2
gas supply line A is changed to B.
Note: The behavoir for the following case is shown in Table 3.
After the CO
2
gas supply line A is changed to B by CO
2
gas automatic changer function, the CO
2
gas supply line B is changed to A manually without pressing the Buzzer key.
Table 3
Situation
CO
2
gas
Supply line Cylinder A Cylinder B
CO
2
gas supply line indicator
A B
Message display field
1
CO
2
gas supply line A is changed to B automatically.
Reverse video and blink
Light on
Err01:
CO
2
Gas Empty
(and buzzer)
2
Without pressing the
Buzzer key, long-pressed CO
2 gas supply line select key
Err01: off
2
Gas Empty
(and buzzer)
78
STD GAS AUTO CALIBRATION KIT (OPTION)
When a STD gas auto calibration kit MCO-SG is installed, by connecting standard gas cylinder for calibration, it is possible to calibrate CO
2
density manually.
1.
Connect a standard gas cylinder to connecting port for standard gas cylinder on lower right side of the CO
2
incubator. Since a Standard gas cylinder is
Lower right side used as a standard of exact density during CO
2 density calibration, prepare a standard gas cylinder that is same as the set CO
2
density.
Note: There is not the problem to remain connected standard gas cylinder after finished CO
2 gas density calibration.
2.
Press Menu key to lead the Menu screen.
3.
Press Tools #1 key to lead the Tools #1 screen.
4.
Press STD Gas Setting key to lead the STD Gas
Setting screen.
Connecting port for standard gas cylinder
79
STD GAS AUTO CALIBRATION KIT (OPTION) screen.
5.
On the STD Gas Setting screen, input CO
2 density of the connected standard CO
2
gas cylinder.
Press Apply key to save the input value. The display returns to the Tools #1 screen.
Settable range: 4.0 %~21.0 %.
Factory setting: 5.0 %.
Note: Don’t turn OFF CO
2
STD Gas Density slide key.
6.
Press STD Gas Calibration key to lead the STD
Gas Calibration screen.
7.
On the STD Gas Calibration Step1 screen, system check starts automatically. If the system is normal, display leads the STD Gas Calibration
Step2 screen.
8.
On the STD Gas Calibration Step2 screen, press
OK key to lead the STD Gas Calibration Step3
80
9.
On the STD Gas Calibration Step3 screen, CO
2 density calibration starts. Calibration go to Step5
(Procedure 10 ) automatically.
10.
After completion of CO
2
density calibration, display leads the STD Gas Calibration Step5 screen. CO
2
incubator returns to the normal operation.
11.
On the STD Gas Calibration Step5 screen, press OK key to return to the Tools #1 screen. On the Tools #1 screen, press Top key to return to the
Top screen.
81
ROUTINE MAINTENANCE
To use this unit in a clean condition, clean the chamber and all the inner attachments at least once a month.
1. Remove all the inner attachments by the procedures shown on page 23.
2. Clean the chamber and all the inner attachments by the procedures shown on page 22.
3. Install all the inner attachments by the procedures shown on page 25.
● When there is excessive dirt, contact our sales representative or agent.
82
ALARMS, SAFETY, AND SELF-DIAGNOSIS
The incubator supports the following alarms, safety functions, and self-diagnostic functions. If an error from Err05 to Err18, or Err56 is activated, contact our sales representative or agent.
Table 4 Alarms, safety, and self-diagnosis for culture operations
Alarm or safety function
High limit temperature alarm
The chamber temperature exceeds the high limit alarm temperature set value.
“Over Heat” is displayed alternately in normal characters and reverse video in the Over heat display.
Automatic set temperature alarm
Automatic set
CO
2
density alarm
The chamber temperature is out of the automatic set temperature alarm setting range (±1.0 o C to ±5.0 o C).
The chamber CO
2 density is out of the automatic set CO
2
density alarm setting range (±0.5 % to ±5.0 %).
Continuous tone
“Warning: High Temp” or
“Warning: Low Temp” is displayed in the message display field.
Intermittent tone after set alarm delay time (0 min to
15 min) has elapsed
Intermittent
“Warning: High CO2 Density” or
“Warning: Low CO2 Density” is displayed in the message display field. tone after set alarm delay time (0 min to
15 min) has elapsed
Auto-return
On screens other than the Top screen, there is no key operation for approx. 90 s.
(When the sleep function is ON)
After sleep function is turned ON, there is no alarm/error and key operation for approx. 90 s.
(Return to the “Top screen”.)
ON after set alarm delay time (0 min to 15 min) has elapsed
ON after set alarm delay time (0 min to 15 min) has elapsed
-----
-----
----- ----- -----
Door alarm The outer door is open.
“Door: Open” is displayed alternately in normal characters and reverse video in the outer door (opening/closing) display.
Intermittent tone after set alarm delay time (1 min to
30 min) has elapsed
-----
The CO
2 valve is closed.
The heater turns OFF after 1 min.
Door lock error
CO
2
gas cylinder empty
(When an optional MCO-170EL is installed)
Outer door is opened when it is auto- locked by electric lock.
“Err20: Door Lock Failure” is displayed in the message display field.
The CO
2
density does not increase when the CO
2
valve is opened.
“Err01: CO2 Gas Empty” is displayed in the message display field.
Intermittent tone
⸗
ON
⸗
UV lamp OFF
-----
Chamber temperature sensor error
Sensor box temperature sensor error
The chamber temperature sensor is disconnected.
The chamber temperature sensor is short-circuited.
The sensor box temperature sensor is disconnected.
The sensor box temperature sensor is short-circuited.
“Err05: Temp Sensor Open” is displayed in the message display field.
“Err06: Temp Sensor Short” is displayed in the message display field.
“Err07: CO2 Box Temp Sensor Open” is displayed in the message display field.
“Err08: CO2 Box Temp Sensor Short” is displayed in the message display field.
⸗
⸗
⸗
⸗
⸗
⸗
⸗
⸗ Heater OFF.
Heater OFF.
CO
2
valve is closed.
CO
2
valve is closed.
Ambient temperature sensor error
CO
2
sensor error
Main heater error
Bottom heater error
Door heater error
Sensor box heater error* 1
Heater SSR burnout* 1
The ambient temperature sensor is disconnected.
The ambient temperature sensor is short-circuited.
“Err09: AT Sensor Open” is displayed in the message display field.
“Err10: AT Sensor Short” is displayed in the message display field.
The Vref or Vgas output voltage for the
CO
2
sensor is abnormal.
“Err11: CO2 Sensor Vref Abnormal” or
“Err12: CO2 Sensor Vgas Abnormal” is displayed in the message display field.
Main heater burnout occurs or the main heater SSR is short-circuited.
“Err13: Main Heater Abnormal” is displayed in the message display field.
Bottom heater burnout occurs or the bottom heater SSR is short-circuited.
“Err14: Humidity Heater Abnormal” is displayed in the message display field.
Door heater burnout occurs or the door heater SSR is short-circuited. a) High limit temperature alarm is activated. b) The sensor box heater burnout occurs or the sensor box heater SSR is short-circuited.
“Err15: Door Heater Abnormal” is displayed in the message display field.
“Err16: CO2 Box Heater Abnormal” is displayed in the message display field. a) High limit temperature alarm is activated. b) Main, bottom, door, or sensor box heater SSR burnout occurs.
“Err17: Heater SSR Open” is displayed in the message display field.
⸗
⸗
⸗
⸗
⸗
⸗
⸗
⸗
⸗
⸗
⸗
⸗
⸗
⸗
⸗
⸗ -----
-----
CO
2
valve is closed.
-----
-----
-----
-----
-----
*1: After a while after the high limit temperature alarm is activated, Err16 (Sensor box heater error) and
Err17 (Heater SSR burnout) are activated.
83
ALARMS, SAFETY, AND SELF-DIAGNOSIS
Alarm or safety function
UV lamp failure The UV lamp burns out.
“Err18: UV Lamp Abnormal” is displayed in the message display field.
Intermittent tone
ON -----
New UV lamp replacement
The accumulated ON time reaches approx. 5,000 h.
“Warning: UV Bulb Life” is displayed in the message display field.
----- ----- -----
Communication error
Warming-up of gas control
When communication between LCD touch panel and control substrate is died out or unstable.
After power switch is turned ON, under warming-up before temperature is stable and gas control is enabled.
“Err56: Communication Failure” is displayed in the message display field.
“Status: Gas sensor initializing” is displayed in the message display field.
ON: Non-interlock with Buzzer key
ON ON
----- ----- -----
----- ----- -----
● Table 5~7 show the behavior of the alarm (buzzer) and Ring Back function when pressing Buzzer key.
Table 5 In the cases of other than table 6 or table 7.
Remote Alarm setting
Ring Back setting
Buzzer from CO
2
incubator
When pressing the Buzzer key
When the Ring Back set time passes
Remote Alarm
When pressing the Buzzer key
When the Ring Back set time passes
ON
ON
(Under continuation)
OFF: Interlock with Buzzer key not canceled)
ON
OFF
Note: Resolve the cause of the alarm in reference to page 83-85 because the alarm itself is not deactivated by pressing Buzzer key.
Table 6 In the cases of high limit temperature alarm or Err38 (The outer door opens during H
2
O
2 decontamination; refer to next page).
Remote Alarm setting
Ring Back setting
Buzzer from CO
2
incubator
When pressing the Buzzer key
When the Ring Back set time passes
Remote Alarm
When pressing the Buzzer key
When the Ring Back set time passes
ON: Non-interlock with Buzzer key
OFF: Interlock with Buzzer key
ON
OFF
ON
OFF
ON
ON
(Under continuation)
ON
(Continue)
ON
(Under continuation)
Note: Close the outer door when Err38 is activated.
Table 7 In the cases of Err01 (CO
2
gas cylinder empty), Err11, 12 (CO
2
sensor error), Err18 (UV lamp failure) or door alarm* 2 .
Remote Alarm setting
Ring Back setting
Buzzer from CO
2
incubator
When pressing the Buzzer key
When the Ring Back set time passes
Remote Alarm setting
When pressing the Buzzer key
When the Ring Back set time passes
ON: Non-interlock with Buzzer key
ON
OFF
OFF
(Alarm is
OFF: Interlock with Buzzer key
ON canceled)
OFF
*2: In the door alarm, the remote alarm does not work.
OFF
(Alarm is already canceled)
OFF
(Alarm is canceled* 2 )
OFF
(Alarm is already canceled* 2 )
Note: When Err01 is activated, connect the new CO
2
gas cylinder and press the Buzzer key to stop the buzzer. In addition, when the optional MCO-21GC is installed and the gas supply is switched to the reserve gas cylinder, press the Buzzer key and replace the gas cylinder.
84
Table 8 Alarms and Safety functions for H
2
O
2
decontamination
Alarm or safety function
The H
2
O
2
generator is not connected.
“Err31: H2O2 Unit Not
Connected” is displayed in the message display field.
System check error at start of H
2
O
2 decontamination
There is no H
2
O
2
solution or the
H
2
O
2
level sensor has failed (or is disconnected).
“Err32: Low H2O2 Level” is displayed in the message display field.
Failure during warming-up
Failure during H
2
O
2 decontamination
Failure during H
2
O
2 gas resolve by UV.
----- -----
----- -----
2
2
decontamination
decontamination is cancelled.
The door is not closed.
“Err33: Outer Door Open” is displayed in the message display field.
----- ----- 2
decontamination is cancelled.
Power was interrupted.
The outer door is open.
The volume of H
2
O
2 abnormal.
generated is
Power was interrupted during H
2
O decontamination.
2
After power is restored, return to the “Top Display”.
----- -----
“Err38: Door Lock Failure” is displayed in the message display field.
Continuous tone
(when the outer door is open.)
-----
ON
(when the outer door is open.)
H
2
O
2
decontamination is cancelled.
“Err34: H2O2 Volume” is displayed in the message display field.
Intermittent tone with
15min delay
ON with 15 min delay
Moves to UV resolve.
After power is restored, “Err35:
Power Failure” is displayed in the message display field.
Intermittent tone
ON
· During power failure, outer door is locked by electric lock.
· After power is restored, moves to UV resolve.
The outer door is open.
“Err38: Door Lock Failure” is displayed in the message display field.
Continuous tone
(when the outer door is open.)
The UV lamp failed during H
2
O
2 gas resolve by UV.
“Err36: UV Lamp Failure” is displayed in the message display field.
Intermittent tone
ON
(when the outer door is open.)
· H
2
O
2
mist generation is cancelled.
· Resolve time is extended.
ON
Resolve time is extended.
Power was interrupted during H
2
O gas resolve by UV.
2
After power is restored, “Err37:
Power Failure” is displayed in the message display field.
-----
-----
· During power failure, outer door is locked by electric lock.
· After power is restored, UV resolve is repeated.
The outer door is open.
“Err38: Door Lock Failure” is displayed in the message display field.
Continuous tone
(when the outer door is open.)
ON
(when the outer door is open.)
Resolve time is extended.
Table 9 Alarm and Safety functions for STD Gas Calibration
Alarm or safety function
System check error at start/operating of automatic CO
2 density calibration
The gas pressure of CO
2
gas line for the standard gas calibration is less than the specified pressure.
“STD Gas Calibration Error” and
“Err41: STD Gas Empty” are displayed in the center of the
LCD touch panel.
Intermittent tone
ON
The STD Gas calibration is cancelled.
Note: When an optional electric lock MCO-170EL is installed, an unlock key is provided in order to unlock the outer door during a power interruption or in case the electric lock fails. Always store this key in a safe place. It is recommended that you make a note of the key symbol and number in case the key is lost.
WARNING
Do not use the unlock key to unlock the outer door during H
2
O
2
decontamination or during H
2
O
2 gas resolve by UV. Doing so may cause harm to health from H
2
O
2
gas leakage.
85
TROUBLESHOOTING
If the incubator does not seem to be working properly, check the following items before calling for service.
Symptom
The incubator does not operate at all.
Items to check and countermeasures
Is the incubator plugged in?
Is there a power outage, or has a circuit breaker interrupted the power?
An alarm is activated.
The chamber temperature does not match the set value.
The chamber humidity does not rise.
The removable power supply cord is connected to the port attached on the lower right side of the cabinet.
[When starting operation]
Does the chamber temperature match the set value?
Does the CO
2
gas density in the chamber match the set value?
(1) Is the secondary pressure for the gas regulator at the specified value of
0.03 MPa(G) ~ 0.1 MPa(G) (0.3 kgf/cm 2 (G) ~ 1 kgf/cm 2 (G), 4.4 psi(G) ~ 14.5 psi(G))?
(2) Is the gas tube properly connected?
[During operation]
Is the high limit alarm temperature set at least 1 C higher than the chamber set temperature?
Has the temperature setting been changed? Has the outer door been left open for a long time? Has a low-temperature object been placed in the chamber? If any of these is the case, the alarm will be automatically cleared if you wait.
Has the gas tube come loose, or is there a gas leak?
Has the CO
2
gas density setting been changed?
Is the gas cylinder empty? Check the primary pressure of the gas cylinder once a week. (When the primary pressure is 3.8 MPa(G) (38 kgf/cm 2 (G), 551 psi(G)) or lower, it is a sign that there is little gas remaining. Replace the cylinder soon.)
Is the incubator operating beside the appliance that generates the electromagnetic wave?
Is the ambient temperature less than 5 C different from the set value for the chamber temperature?
Is the outer door closed with the inner door left open?
Is the incubator operating beside the appliance that generates the electromagnetic wave?
Is there enough water in the humidifying pan? (Be sure to use sterile distilled water.)
The CO
2
gas density does not match the set value.
A large quantity of CO
2
gas is being consumed.
Is the secondary pressure for the gas regulator at the specified value of 0.03 MPa(G)
~ 0.1 MPa(G) (0.3 kgf/cm 2 (G) ~ 1 kgf/cm 2 (G), 4.4 psi(G) ~ 14.5 psi(G))?
Is the gas tube blocked?
Is the duct securely attached? Attach the duct properly to the 4 points hooks. (Fig. 1 on page 88)
Is the fan attached properly? Confirm if the fan is pushed all the way to the motor shaft.
(Fig. 2 on page 88)
Is the incubator operating beside the appliance that generates the electromagnetic wave?
Are the outer and inner doors being frequently opened and closed?
Check whether gas is leaking from connectors due to deterioration of the gas tube, or whether there may be any pinhole leaks. The gas tube is a replaceable part, and it is recommended that it be replaced once a year.
Is the packing seal for the inner door defective?
Is the access hole open?
86
Symptom
Normal cultures are not possible, and the CO
2
gas density is suspect.
CO
2
gas is not being injected.
Items to check and countermeasures
Is the ambient air environment around the incubator normal? Is there a source of polluted gas in the vicinity?
The CO
2
gas density is slow to recover.
The CO
2 control method for the incubator is the ON-OFF method. CO
2
gas is intermittently injected as the gas density in the chamber approaches the set value.
Injections may be stopped for periods of approximately 15 seconds, but that is not an error.
The gas is not injected until the temperature of the CO
2
sensor becomes stable enough approx. 1 hour, after turning ON the power switch or recovering from power failure.
A HEPA filter is used for the incubator CO
2
gas piping. If gas density is slow to recover when the CO
2
gas pressure is normal, it is possible that the HEPA filter may be clogged.
Contact our sales representative or agent.
Is there little gas remaining in the CO
2
gas cylinder?
Is the secondary pressure for the gas regulator at the specified value of 0.03 MPa(G)
~ 0.1 MPa(G) (0.3 kgf/cm 2 (G) ~ 1 kgf/cm 2 (G), 4.4 psi(G) ~ 14.5 psi(G))?
Is the gas tube blocked?
Is the duct securely attached? Attach the duct properly to the 4 points hooks. (Fig. 1)
Is the fan attached properly? Confirm if the fan is pushed all the way to the motor shaft.
(Fig. 2)
UV lamp lights when the outer door is open.
The outer door does not open.
Does something push the door switch?
When the optional MCO-170EL is installed:
When the power switch is OFF, the electric lock is locked and the outer door does not open. Either turn ON the power switch or use the accessory unlock key to override the electric lock.
During decontamination the outer door is electrically locked and does not open.
87
TROUBLESHOOTING
Symptom
H
2
O
2 decontamination cannot be performed.
In H
2
O
2
decontamination cycle,
“Err34: H2O2 Volume” is activated.
Items to check and countermeasures
Are the optional MCO-HP, the optional MCO-170HB and the MCO-170EL installed?
Is the UV lamp burned out? If the UV lamp is burned out, H
2
O
2 decontamination will not be possible.
Is the H
2
O
2
generator cable properly connected?
Has the entire bottle of the H
2
O
2
reagent been used?
Is the duct securely attached? Attach the duct properly to the 4 points hooks. (Fig. 1)
Is the fan attached properly? Confirm if the fan is pushed all the way to the motor shaft.
(Fig. 2)
Is the H
2
O
2
generator securely installed? Set the 2 pins of the H
2
O
2
generator in the 2 holes at the left bottom of the duct. (Fig. 3)
Is it the end-of-life of the H
2
O
2
generator? If the total operating time exceeds 5,000 hours, replace the H
2
O
2
generator.
1.Position the center hole of the fan with the projection of the motor shaft.
And insert it deeply.
Fig. 1
2.Lightly turn the fan manually to make sure that it does not touch the rear panel.
3.Lightly pull the fan manually to make sure that it is installed.
Fig. 2 Fig. 3
Note: If the problem still has not been solved after trying the above checks and countermeasures, or for any problems not covered here, contact our sales representative or agent.
Keep an electric product which emits an electromagnetic wave away from this product. A noise from an electromagnetic wave may cause malfunction to this product.
88
DISPOSAL OF UNIT
When disposing of the CO
2
incubator, contact our sales representative or agent.
WARNING
The CO
2
incubator must be dismantled and disposed of by qualified personnel only . If the CO
2 incubator is left where outsiders enter, it may result unexpected accident (for example, children to become locked inside).
Before disposing the CO
2
incubator with biohazardous danger, decontaminate the CO
2
incubator to the extent possible by the user.
廢電池
請回收
Label indication is obliged to comply with Taiwanese battery regulation.
89
SPECIFICATIONS
Product name
External dimensions
Internal dimensions
Interior volume
Exterior
Interior
Outer door
Inner door
Trays
Access port
Insulation
Heating system
Heater
Humidifying system
Temperature controller
Temperature display
CO
2
controller
CO
2 density display
Air circulation
Air filter
UV lamp
Alarms
Remote alarm contacts
CO
2
inlet connection
CO
2
inlet pressure
Weight
Accessories
CO
2
Incubator MCO-230AICUVL
W770 mm x D730 mm x H905 mm (W30.3 inch x D28.7 inch x H35.6 inch)
W643 mm x D523 mm x H700 mm (W25.3 inch x D20.6 inch x H27.6 inch)
230 L (8.12 cu.ft.)
Painted steel (Rear cover has no paint)
Stainless steel containing copper
Painted steel
Tempered glass
4 trays made of stainless steel containing copper
W620 mm x D450 mm x H12 mm (W24.4 inch x D17.7 inch x H0.47 inch)
Maximum load: 7 kg/tray
Inner diameter: 30 mm (1.18 inch), On the back side
Styrene AcryloNitrile copolymer
DHA system (heater jacket + air jacket system)
345 W
Natural evaporation with humidifying pan
PID control system
Digital display
PID control system
Digital display
Fan assisted
0.3 m, Efficiency: 99.97 % or higher
4 W x 1 (ozone-free emission)
Automatic set temperature alarm, Automatic set CO
2
density alarm,
High limit temperature alarm, CO
2
gas, various sensor/heater alarms
Allowable contact capacity: DC 30 V, 2 A
4 mm to 6 mm (0.157 inch to 0.236 inch) diameter tube can be connected
0.03 MPa(G)~0.1 MPa(G) (0.3 kgf/cm 2 (G)~1 kgf/cm 2 (G), 4.4 psi(G)~14.5 psi(G))
90 kg
1 removable power supply cord, 1 power supply cord cover plate, 4 trays
1 gas tube, 1 humidifying pan, 2 tube bands
90
Product name
Optional accessories
(Refer to table 10)
CO
2
Incubator MCO-230AICUVL
H2O2 decon board (MCO-170HB)
Electric lock (MCO-170EL)
H
2
O
2
generator (MCO-HP)
Optional accessories
(Refer to table 11 and 12)
Optional accessories
Double stacking bracket (MCO-170PS)
Stacking plate (MCO-230SB)
H
2
O
2
reagent (MCO-H2O2)
Gas regulator (MCO-010R)
Gas auto changer (MCO-21GC)
STD gas auto calibration kit (MCO-SG)
Tray (MCO-230ST: same as that of standard accessory)
Half tray (MCO-35ST)
Roller base (MCO-230RB)
Interface board (MCO-420MA)
Interface board (MTR-L03)*; For LAN
Interface board (MTR-480)*; For RS-232C/RS-485
*Only for the Data acquisition system MTR-5000 user.
Note : Refer to the updated catalog when ordering an optional component.
Designs and specifications are subject to change without notice.
Table 10 Optional accessories for each function
H
2
O
2
generator (MCO-HP)
To decontaminate by H
2
O
2.
H2O2 decon board (MCO-170HB)
Electric lock (MCO-170EL)
To lock the outer door Electric lock (MCO-170EL)
Table 11 Required bracket/plate for each incubator combination of double stacking
MCO-230AIC series MCO-18AC series
Upper product
Lower product
MCO-170AIC series
MCO-170M series
MCO-19AIC series
MCO-19M series
MCO-170AICD series
MCO-230AIC series
Bracket Plate
Double stacking bracket
MCO-170PS
Stacking plate
MCO-230SB
Table 12 Required bracket/plate for each incubator combination of double stacking
Upper product
Lower product MCO-230AIC series
MCO-230AIC series
MCO-20AIC series
Bracket Plate
Double stacking bracket
MCO-170PS
Stacking plate
MCO-230SB
91
PERFORMANCE
Product name
Model number
Temperature control range
Temperature distribution
CO
2
Incubator MCO-230AICUVL
MCO-230AICUVL-PA
Ambient temperature+5 o C to 50 o C* (ambient temperature: 5 o C to 35 o C)
±0.25 o C (ambient temperature: 23 o C, setting: 37 o C, CO
2
: 5 %, no load)
±0.1 o C (ambient temperature: 23 o C, setting: 37 o C, CO
2
: 5 %, no load) Temperature variation
CO
2
control range
CO
2
variation
Chamber humidity
0 % to 20 %
±0.15 % (ambient temperature: 23 o C, setting: 37 o C, CO
2
: 5 %, no load)
Applicable environment condition
95 %R.H.±5 %R.H.
Temperature: 5 o C to 35 o C, Humidity: 80 %R.H. max.
(The designed performance may not be obtained
If the ambient temperature is equal or less than 15 o C)
Noise level
Power consumption
25 dB (A scale)
Max. 430 W
Heat emission Max. 1,250 kJ/h
Rated voltage, frequency AC 110 V-120 V, 60 Hz
Amperage Max. 3.8 A
*When set temperature is 37 o C, ambient temperature must be 32 o C or less. Regardless of ambient temperature, the maximum of temperature control range is always 50 o C.
Note: Based on our measuring method.
92
CAUTION
Please fill in this form before servicing.
Hand over this form to the service engineer to keep for his and your safety.
Safety check sheet
1. Unit contents :
Risk of infection:
Risk of toxicity:
Risk from radioactive sources:
□
Yes
□
No
□
Yes
□
No
□
Yes
□
No
(List all potentially hazardous materials that have been stored in this unit.)
Notes :
2.
Contamination
of the unit
Unit interior
No contamination
Decontaminated
Contaminated
Others:
□
Yes
□
No
□
Yes
□
No
□
Yes
□
No
3. Instructions for safe repair/maintenance/disposal of the unit a) The unit is safe to work on b) There is some danger (see below)
□
Yes
□
No
□
Yes
□
No
Procedure to be adhered to in order to reduce safety risk indicated in b) below.
Date :
Signature :
Address, Division :
Telephone :
Date of Installation : Product name :
CO
2
incubator
Model No.
MCO-
Serial number :
Please decontaminate the unit yourself before calling the service engineer.
93
1-1-1 Sakada, Oizumi-machi, Ora-gun, Gunma 370-0596, Japan
© PHC Corporation 2018
Printed in Indonesia
LDCL-041300-0
N0418-0
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