epBlue™ with EasyCon

epBlue™ with EasyCon
hmanual
ual
EasyCon
EN)
epBlue™ with EasyCon
Software manual
Software version 40.4
Copyright© 2015 Eppendorf AG. All rights reserved, including graphics and images. No part of this
publication may be reproduced without the prior permission of the copyright owner.
Focus Diagnostic® is a registered trademark of Focus Diagnostic, Inc., USA.
Qiagen® and Rotor-Disk® are registered trademarks of Qiagen GmbH, Germany.
Excel® and Windows® are registered trademarks of Microsoft Corporation in the United States and other
countries.
epMotion®, Eppendorf Tubes® epT.I.P.S.®, and Eppendorf ThermoMixer® are registered trademarks of
Eppendorf AG, Germany.
Eppendorf® and the Eppendorf logo are registered trademarks of Eppendorf AG, Germany.
Registered trademarks and protected trademarks are not marked in all cases with ® or ™ in this manual.
The software of the device (firmware) contains open source software. License information is available on
request from Eppendorf AG.
Only for epMotion M5073 and 5075m: Limited Use Label License NOTICE TO PURCHASER; LIMITED
LICENSE FOR RESEARCH USE ONLY.
This product and its use may be covered by one or more patents owned by Gen-Probe Incorporated. The
purchase price for this product includes only limited, nontransferable rights under certain claims of certain
patents owned by Gen-Probe Incorporated to use this product for research purposes only. No other rights
are conveyed. Purchaser is not granted any rights under patents of Gen-Probe Incorporated to use this
product for any commercial use. Further information regarding purchasing a license under patents of
Gen-Probe Incorporated to use this product for any other purposes, including, without limitation, for
commercial use, may be obtained by contacting Gen-Probe Incorporated, Attn: Business Development
Department, 10210 Genetic Center Drive, San Diego, California 92121-4362, U.S.A.
5075 901.250-01/082015
Table of contents
epBlue™ with EasyCon
English (EN)
Table of contents
1
Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.1
Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2
Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2.1
Danger symbols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2.2
Danger levels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.3
Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.4
Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.5
Glossary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1.6
Version overview for the software manual epBlue with EasyCon. . . . . . . . . . . . . . . . . . . . . . . . 12
2
Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3
Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.1
Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.1.1
epBlue . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.1.2
Assistants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4
Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
4.1
Starting and exiting epBlue . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
4.1.1
Starting epBlue . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
4.1.2
Exiting epBlue and switching off EasyCon Tablet . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
4.2
Initial steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
4.2.1
Using touchscreen and mouse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
4.2.2
Using the screen keyboard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
4.2.3
File menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4.2.4
Status area. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
4.2.5
Enlarging the view . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5
epBlue Application Runner - Carrying out the application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.1
Opening an application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.2
Preparing the application run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.2.1
Selecting the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.2.2
Entering the number of samples. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5.2.3
Setting the HEPA air filter and the UV lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5.2.4
Positioning the labware on the epMotion worktable . . . . . . . . . . . . . . . . . . . . . . . . . . 26
5.2.5
Setting the run parameters for the optical sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
5.2.6
Setting the run parameters for the labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.2.7
Entering the filling volume of the labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
5.3
Starting the application. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
5.4
Controlling the application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
5.5
Ending the application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
6
epBlue Log viewer - Managing application logs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
6.1
Displaying protocols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
6.2
Saving the log as a PDF file . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
3
4
Table of contents
epBlue™ with EasyCon
English (EN)
7
epBlue Application Editor - Creating and editing applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
7.1
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
7.2
Creating an application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
7.2.1
Menu bar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
7.2.2
Creating an empty application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
7.2.3
Copying applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
7.2.4
Saving applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
7.2.5
Positioning labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
7.2.6
Defining the application parameters of the labware. . . . . . . . . . . . . . . . . . . . . . . . . . . 40
7.2.7
Defining procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
7.2.8
Checking an application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
7.3
Commands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
7.3.1
Sample transfer command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
7.3.2
Reagent transfer command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
7.3.3
Dilute command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
7.3.4
Pool command. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
7.3.5
Pool one destination command. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
7.3.6
Pierce command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
7.3.7
Comment command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
7.3.8
Exchange command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
7.3.9
Magnetic separation command. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
7.3.10 Mix command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
7.3.11 Number of samples command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
7.3.12 Temperature command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
7.3.13 ThermoMixer command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
7.3.14 Transport command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
7.3.15 User intervention command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
7.3.16 External process command. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
7.3.17 Wait command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
7.4
Pattern . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
7.4.1
Menu bar in the Edit Pattern window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
7.4.2
Defining regular patterns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
7.4.3
Defining irregular patterns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
7.5
Optical sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
7.5.1
Measuring the filling level of the labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
7.5.2
Detecting pipette tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
7.5.3
Checking the labware on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
8
epBlue Application Editor - Managing applications and folders. . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
8.1
Menu bar and start screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
8.2
Copying and deleting applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
8.2.1
Copying applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
8.2.2
Deleting an application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
8.3
Changing the properties and name of the application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
8.4
Importing and exporting applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
8.4.1
Importing applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
8.4.2
Exporting applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
8.5
Saving an application as a PDF file. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
8.6
Saving an application for a different epMotion model . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
Table of contents
epBlue™ with EasyCon
English (EN)
9
epBlue Labware Editor - Creating and managing labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
9.1
Overview of labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
9.2
Managing labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
9.2.1
Menu bar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
9.2.2
Deactivating and activating labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
9.2.3
Deleting labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
9.2.4
Importing labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
9.2.5
Exporting labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
9.2.6
Viewing the properties of the labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
9.2.7
Setting the bottom tolerance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
9.3
Creating labware combinations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
9.3.1
Creating a labware combination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
9.3.2
Creating a labware combination based on existing labware . . . . . . . . . . . . . . . . . . . . 99
9.3.3
Equipping the racks, thermoracks, and ReservoirRack modules with individual vessels
100
9.3.4
Equipping the ReservoirRack with reservoirs and ReservoirRack modules . . . . . . . . 102
9.4
Labware library . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
9.4.1
Loading labware definitions from the Internet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
9.4.2
Request labware definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
9.4.3
Add a labware definition to a labware library . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
9.5
Special labware features in epBlue . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
9.5.1
Location B0 on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
9.5.2
Tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
9.5.3
MTP plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
9.5.4
PCR plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
9.5.5
epT.I.P.S. Motion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
9.5.6
Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
9.5.7
Rack 96 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
9.5.8
Rack LC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
9.5.9
ReservoirRack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
9.5.10 ReservoirRack Module TC. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106
9.5.11 Thermoracks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106
9.5.12 Thermorack Rotor/Tubes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
9.5.13 TipHolder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
9.5.14 Thermoadapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
9.5.15 Universal Disc by Focus Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
9.5.16 Thermoblocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
9.5.17 Thermoblock PCR 96 OC. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
5
6
Table of contents
epBlue™ with EasyCon
English (EN)
10 epBlue settings - Checking components and making the basic settings . . . . . . . . . . . . . . . . . . . . 109
10.1 Tool interlock. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
10.1.1 Lock the tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110
10.1.2 Unlocking the tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110
10.2 Dosing device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110
10.2.1 Reading out the tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110
10.3 Gripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
10.3.1 Moving the gripper arms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
10.4 UV lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
10.4.1 Decontaminating the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
10.5 HEPA air filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
10.5.1 Switching on the HEPA air filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
10.5.2 Switching off the HEPA air filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114
11 epBlue Administration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115
11.1 epBlue Administration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115
11.1.1 Administration Starting epBlue. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115
11.1.2 Exit epBlue Administration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115
11.2 Changing the device name . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115
11.3 Print templates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
11.3.1 Creating a print template . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
11.3.2 Saving print templates as default . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
11.3.3 Deleting a print template . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
11.4 Data backup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118
11.4.1 Backing up the database . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118
11.4.2 Restoring the database . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119
11.4.3 Importing a database from epBlue version 40.X . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120
11.5 Temperature control unit X . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120
11.6 Levelsensor options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
11.7 ThermoMixer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
11.7.1 Checking the mixing function of the Eppendorf ThermoMixer module. . . . . . . . . . . 123
11.7.2 Checking the temperature control of the Eppendorf ThermoMixer module . . . . . . . 124
11.8 Magnetic separation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125
12 epBlue - Software error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127
12.1 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127
13 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155
13.1 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 156
Operating instructions
epBlue™ with EasyCon
English (EN)
1
1.1
Operating instructions
Using this manual
Your epMotion operating manual consists of hardware instructions and software instructions. Separate
instructions exist for some optional software modules.
The operating manual is part of the product.
The current version of the operating manual can be found on our website: www.eppendorf.com.
 Read the operating manual in full before using the device.
 Store the operating manual at an easily accessible location.
 The device may only be transferred with the operating manual.
 If the operating manual is lost, replace it immediately. Please contact Eppendorf AG for further details.
1.2
Danger symbols and danger levels
1.2.1
Danger symbols
The safety instructions in this manual appear with the following danger symbols and danger levels:
1.2.2
Biohazard
Explosive substances
Strong magnetic field
UV radiation
Electric shock
Heavy load
Cuts
Hot surface
Hazard point
Material damage
Danger levels
DANGER
Will lead to severe injuries or death.
WARNING
May lead to severe injuries or death.
CAUTION
May lead to light to moderate injuries.
NOTICE
May lead to material damage.
7
8
Operating instructions
epBlue™ with EasyCon
English (EN)
1.3
Symbols used
Depiction
Meaning
1.
2.
Actions in the specified order

Actions without a specified order
•
List
Text
Display text or software text
Additional information
1.4
Abbreviations used
CSV
Comma Separated Value
DNA
Deoxyribonucleic acid
DWP
Deepwell plate
epT.I.P.S.
Eppendorf Totally Integrated Pipetting System
HEPA
High Efficiency Particulate Air (filter)
LH
Liquid Handling
LIMS
Laboratory Information Management System
MTP
Microplate
PCR
Polymerase chain reaction
PDF
Portable Document Format
TMX
Eppendorf ThermoMixer module
USB
Universal Serial Bus
UV
Ultraviolet radiation
Operating instructions
epBlue™ with EasyCon
English (EN)
1.5
Glossary
A
Administrator
User possessing the rights for the administration of epBlue.
Application
Program for a dedicated application. An application comprises the procedure and the equipping of the
worktable.
C
Cleanbench
Class 2 safety cabinet for personal security and product protection. A laminar air flow prevents germs from
entering the cabinet from the ambience. The air flow also prevents aerosols that contain microorganisms
from escaping the cabinet.
CleanCap
Optional epMotion equipment. The CleanCap includes a HEPA filter and at least one UV lamp.
Command
Step in a procedure. Various parameters are set for commands. Each command triggers an action, e.g., the
transport of liquids.
Control device
Device for controlling the epMotion. Depending on the equipment of the epMotion, a MultiCon PC or a
EasyCon Tablet is available.
CSV file
Text file for saving and exchanging structured information.
D
Destination labware
Rack or plate with destination positions.
Destination position
Position in the labware that exclusively is destination of liquid transfers.
Dispensing tool
Tool that aspirates and dispenses liquid. Single-channel dispensing tools and eight-channel dispensing
tools are available for various volume ranges.
E
EasyCon Tablet
Eppendorf EasyCon™ Tablet. You can control your epMotion using the tablet and the epBlue software.
9
10
Operating instructions
epBlue™ with EasyCon
English (EN)
epBlue
Software for creating and administrating applications and labware. The epBlue software is used for
controlling the epMotion.
epT.I.P.S. Motion
Pipette tips of the epMotion. epT.I.P.S.® Motions are available with or without a filter. The abbreviation
stands for Eppendorf Totally Integrated Pipetting System.
F
Filling volume
Maximum volume of a labware. The filling volume is higher than the working volume. The epMotion
removes liquid from the labware up to the filling volume.
G
Gripper
Tool that transports labware.
H
Height adapter
Adapter for low labware. The height differences between the labware is offset to decrease the tool holder
paths, and therefore decrease the cycle time of the application.
HEPA filter
Air filter. The filter prevents particles such as dust and germs from the environment entering the epMotion
workspace.
L
Labware
Racks, plates, tips, etc. which are placed on the worktable.
Location
Area where labware is placed on the worktable.
M
MagSep
System for nucleic acid preparation with the epMotion. The MagSep consists of a magnetic finger module,
an Eppendorf ThermoMixer module, a PrepRack, and reagents.
P
Pattern
Pattern in which liquid is aspirated and dispensed. Patterns are defined in a transfer command. Patterns are
defined as regular patterns and irregular patterns. Patterns are independent in x-direction and y-direction.
Operating instructions
epBlue™ with EasyCon
English (EN)
Procedure
Sequence of commands executed consecutively. Part of an application.
R
Rack
Mount for tubes or pipette tips.
Remaining volume
Volume that cannot be removed from a vessel. Caused by the distance from the pipette tip to the vessel
base, defined in the software, and the defined immersion depth of the pipette tip in the liquid. The pipette
tip cannot aspirate the volume. The remaining volume depends on the vessel geometry.
Reservoir
Reservoirs are used for preparing reagents. Reservoirs are hung in a ReservoirRack or placed directly on
the worktable.
S
SafeRack
Rack with ep.T.I.P.S. Motion pipette tips. The SafeRack features a partition that prevents the contamination
of adjacent tips. If you want to use the tips several times, use SafeRacks.
Source labware
Rack or plate with source positions.
Source position
Position in a labware that exclusively is a source of liquid transfers.
T
Thermal module
Heat plate for labware. The heat plate is integrated in the worktable.
Thermoadapter
Temperature-controlled adapter that is not transported with the gripper. Plates are inserted into the
thermoadapter.
Thermoblock
Temperature-controlled adapter that is transported with the gripper.
There are 2 types of thermoblocks:
• Thermoblocks: Thermoblocks, into which plates are inserted.
• Thermoblocks with plates Thermoblocks that are permanently connected with a plate.
Thermorack
Temperature-controlled rack for smaller vessels, e.g., Safe-Lock tubes for 0.5 mL, 1.5 mL or 2 mL.
Tube
A micro test tube or a single well in a plate.
11
12
Operating instructions
epBlue™ with EasyCon
English (EN)
W
Work volume
Filling volume minus volume of the immerging pipette tip. The epMotion fills the vessels up to the working
volume.
Worktable
epMotion work surface that labware and tools are placed on. In the software, the epMotion worktable is
represented as epBlue worktable.
1.6
Version overview for the software manual epBlue with EasyCon
Order no.: 5075 901.250
Version
Issue date
Change
00
August 2013
• Creation. Software version 40.1.
01
August 2015
• Update to software version 40.4.
Safety
epBlue™ with EasyCon
English (EN)
2
Safety
The intended use and safety instructions sections from the hardware manual apply for using the epMotion
with the epBlue 40.X software.
13
14
Safety
epBlue™ with EasyCon
English (EN)
Product description
epBlue™ with EasyCon
English (EN)
3
3.1
Product description
Features
With the epBlue software you control the epMotion. epBlue consists of several modules.
Which modules are available depends on the features of your epMotion and the specification of the
software.
3.1.1
epBlue
Modules
Description
Application
Editor
• Create applications.
• Manage applications.
Operating manual
(see epBlue Application Editor - Creating and editing
applications on p. 35)
(see epBlue Application Editor - Managing applications and
folders on p. 83)
Application
Runner
• Execute applications.
(see epBlue Application Runner - Carrying out the
application on p. 23)
Labware Editor
• Manage labware.
(see epBlue Labware Editor - Creating and managing
labware on p. 91)
Log Viewer
• Display and save logs.
(see epBlue Log viewer - Managing application logs on
p. 33)
Settings
• Check components of the
device.
• Perform basic settings.
(see epBlue settings - Checking components and making
the basic settings on p. 109)
Administration
• Backup data.
• Create print templates.
(see epBlue Administration on p. 115)
Service
• Access for Eppendorf
service technician.
3.1.2
Assistants
Assistants are programs for a specific application. Assistants are optionally available and described in
separate operating manuals. The following assistants are available for epBlue:
Prep Assistant
• Nucleic acid preparation with Eppendorf MagSep kits. After that, a PCR setup can be performed.
PCR Assistant
• Create PCR master mixes and dilution series for quantification standards, normalization of sample
concentrations, and setting up PCR reactions.
LH assistant
• Normalization of sample concentrations.
15
16
Product description
epBlue™ with EasyCon
English (EN)
Operation
epBlue™ with EasyCon
English (EN)
4
4.1
Operation
Starting and exiting epBlue
4.1.1
Starting epBlue
Prerequisites
• epMotion and EasyCon Tablet were installed by a service technician.
 Switch on the epMotion using the mains/power switch.
The EasyCon Tablet is started at the same time as the epMotion. epBlue starts automatically when the
EasyCon Tablet is ready for operation. The epBlue start screen appears.
Abb. 4-1: epBlue start screen
1
2
3
4
Fig. 4-1:
epBlue start screen
1 File menu
Contains functions that can be accessed in all
applications.
2 Information area
Contains information on the open file.
3 Status area
Status of the epMotion
4 Working area
Contents and applications.
17
18
Operation
epBlue™ with EasyCon
English (EN)
4.1.2
Exiting epBlue and switching off EasyCon Tablet
NOTICE! Data loss due to power-off without shutting down the device first.
The EasyCon Tablet mains/power supply is terminated when the epMotion is switched off.
Failure to first shut down the EasyCon Tablet may result in data loss. This can damage the
EasyCon Tablet.
 Shut down the EasyCon Tablet before switching off the epMotion.
1. In the File menu, select Shut down.
2. If the touchscreen of the EasyCon Tablet is dark, switch off the epMotion at the mains/power switch.
4.2
Initial steps
4.2.1
Using touchscreen and mouse
You can operate the operating unit via the touchscreen or use a USB mouse.
 Connect the mouse to a USB interface on the operating unit.
Tab. 4-1:
Touchscreen/mouse comparison
Touchscreen
Mouse
Tap
Click using the left mouse button
Tap and hold 2 s
Click using the right mouse button
Drag
Drag with the left mouse button pressed down
Tap twice
Double-click using the left mouse button
4.2.2
Using the screen keyboard
Enter the numbers and letters using the screen keyboard.
Abb. 4-2: Screen keyboard
Fig. 4-2:
Screen keyboard
Automatically show the screen keyboard
 epBlue automatically shows a keyboard if you have selected an input field.
Manually show the screen keyboard
 In the File menu, select the Show keyboard entry.
Switch the automatic display of the screen keyboard on and off.
 In the File menu, select the Show keyboard automatically entry.
Operation
epBlue™ with EasyCon
English (EN)
Tab. 4-2:
epBlue key combinations
Enter special characters.
 Press the letter key for 2 s.
Confirm your entry.
 Press the key once.
Switch between lowercase
letters and capital letters.
 To enter a capital letter, press the key once. Then press
the letter.
 To switch the keyboard to all capital letters, press the
key twice.
Switch the keyboard.
 To switch between the English and German keyboards,
press the key once.
Show the screen keyboard.
4.2.3
 Press the key once.
File menu
Abb. 4-3: File drop-down menu
Fig. 4-3:
File drop-down menu
Exit to start screen entry
Show the epBlue start screen.
Sound enabled entry
Switches the system sound on and off.
Menu – Show keyboard
Manually show the screen keyboard.
Shutdown entry
End the program and shutdown the operating
device.
Menu – Show keyboard automatically
Switch the automatic display of the screen
keyboard on and off.
19
20
Operation
epBlue™ with EasyCon
English (EN)
Abb. 4-4: Help drop-down menu
Fig. 4-4:
Help drop-down menu
Device manuals entry
Open the operating manual of the device.
About epBlue entry
Show the epBlue version.
Software manuals entry
Open the operating manual of the software.
4.2.4
Status area
The status area is located in the top right of the screen.
If you click the epMotion in the status area, the following information is displayed:
• epMotion serial number.
• Equipment of the epMotion
• Firmware version
• Status of the epMotion
• Actual temperature of a thermal module or an Eppendorf ThermoMixer module.
Example Info: C3:21°C. The thermal module in location C3 has a temperature of 21°C.
Operation
epBlue™ with EasyCon
English (EN)
4.2.5
Enlarging the view
You can enlarge the image of a set of labware. All positions of the labware become clearly visible. The
enlarging function is a help, e.g. for patterning. You can use the navigation window for better navigation in
the enlarged image.
Abb. 4-5: View window
Fig. 4-5:
View window
Decrease the image in the view window.
Press button or scroll with the mouse wheel.
Enlarge the image in the view window.
Press button or scroll with the mouse wheel.
72 %
Display the image in the view window at the desired percentage.
Show the navigation window.
Press the Cross button and move the navigation window.
Alternatively, press the Ctrl key on the keyboard. Press and hold the left mouse
wheel. Move the navigation window.
21
22
Operation
epBlue™ with EasyCon
English (EN)
Abb. 4-6: Navigation window
Fig. 4-6:
Navigation window
Return to previous view.
Go to next view.
Scale image to 100%.
Scale image to the size of the view window.
Center image in the view window. The enlargement remains unchanged.
Close the navigation window.
epBlue Application Runner - Carrying out the application
epBlue™ with EasyCon
English (EN)
5
epBlue Application Runner - Carrying out the application
This chapter describes how to carry out an application on the epMotion.
5.1
Opening an application
Abb. 5-1: Selecting an application
Fig. 5-1:
Selecting an application
Prerequisites
• epMotion is ready for operation.
• Application Runner is started.
1. Select the application in the Select application area.
Recently used applications can be found in the Recently used area.
Open applications in the Select application area have a red padlock .
The Properties area displays the properties of the selected application.
2. Press the Next button.
The application is opened.
23
24
epBlue Application Runner - Carrying out the application
epBlue™ with EasyCon
English (EN)
5.2
Preparing the application run
5.2.1
Selecting the device
Abb. 5-2: Selection of an available device
Fig. 5-2:
Selection of an available device
NOTICE! Tool collisions with incorrectly positioned labware.
Labware must be positioned on the epMotion worktable as defined in the application.
Tools and labware become damaged in a collision. A collision can lead to sample loss.
 Check the positioning of the labware before starting the application.
In the Available Devices area, the connected epMotion is displayed.
Prerequisites
• epMotion is ready for operation.
1. Select the device for the application run.
2. Press the Next button.
epBlue Application Runner - Carrying out the application
epBlue™ with EasyCon
English (EN)
5.2.2
Entering the number of samples
Abb. 5-3: Process control window
Fig. 5-3:
Process control window
The window Process control appears if the number of samples in the application is variable.
1. Enter the number of samples for the current run.
2. Press the OK button.
5.2.3
Setting the HEPA air filter and the UV lamp
Abb. 5-4: Setting the parameters for the HEPA air filter and the UV lamp
Fig. 5-4:
Setting the parameters for the HEPA air filter and the UV lamp
If your epMotion is equipped with a HEPA air filter and UV lamp, you can define the settings for the HEPA
air filter and the UV lamp.
 Activate or deactivate the HEPA air filter.
 Define whether a decontamination is to be carried out before the application.
The worktable may only be decontaminated if no labware or tools are present on the worktable.
25
26
epBlue Application Runner - Carrying out the application
epBlue™ with EasyCon
English (EN)
5.2.4
Positioning the labware on the epMotion worktable
 Position the labware on the epMotion worktable according to the application.
Information on positioning the labware can be found in the hardware operating manual.
5.2.5
Setting the run parameters for the optical sensor
In the application, you defined the application parameters for the optical sensor. You can enter run
parameters to use other settings of the optical sensor for an individual run. The run parameters overwrite
the application parameters for this run only (see Optical sensor on p. 77).
Abb. 5-5: Setting the run parameters
Fig. 5-5:
Setting the run parameters
Input volumes manually radio button
Detect volumes radio button
Entering run parameters manually
Filling volume measurement by the optical
sensor. The parameters set in the application for
Detect tips slider
the labware are used.
Activate the Detect tips function.
Use required minimum volumes radio button
Check labware placement slider
epBlue assumes that all vessels are filled with the
Activate the Check labware placement function.
minimum required volume for this run. Use this
function for dry runs.
1. Configure the functions of the optical sensor.
2. Press the Run button.
epBlue Application Runner - Carrying out the application
epBlue™ with EasyCon
English (EN)
5.2.6
Setting the run parameters for the labware
You have defined in the labware properties how the optical sensor measures the filling level in the
application. In the run parameters, you can enter a different option for measuring the filling level. The run
parameters overwrite the application parameters for this run only (see Optical sensor on p. 77).
Abb. 5-6: Setting the run parameters for the labware
Fig. 5-6:
Setting the run parameters for the labware
Prerequisites
In the Volume settings, the Detect volumes is activated.
1. Select the labware.
The window with the labware properties appears.
2. For plates, select the mode in which the optical sensor measures the plate filling level. To do so, activate
the Random positions checkbox or the All used positions checkbox.
3. For ReservoirRack modules, highlight the ReservoirRack module in which the optical sensor measures
the filling level. In the table, activate the Level sensor checkbox.
The manual entry field is grayed out. You can no longer enter the volume manually. The optical sensor
measures the volume.
4. To close the labware properties window, press the Next button.
27
28
epBlue Application Runner - Carrying out the application
epBlue™ with EasyCon
English (EN)
5.2.7
Entering the filling volume of the labware
5.2.7.1 Measuring the filling volume with the optical sensor
If the Detect volumes function is active, the optical sensor checks the filling volume after the start.
If the following problems occur, a dialog window appears:
• Filling volume too low.
• Filling volume too high.
• Filling volume cannot be correctly detected.
Abb. 5-7: Checking window
Fig. 5-7:
Checking window
Maximum volume
Maximum filling volume for the position.
Abort button
Aborts the application.
Minimum volume
User input button
Filling volume required to remove the set amount
Entering the filling volume manually
of fluid.
Accept level and continue button
Calculated volume
Accept the calculated filling volume.
Calculated filling volume for the position. The
µL input field
filling volume is calculated from the labware data
If the User Input button has been selected, enter
and the fluid level.
the filling volume manually.
Repeat scan button
Measuring the filling volume again with the
optical sensor
NOTICE! Dispensing error due to refilling of vessels.
The optical sensor only checks the level of the vessels at the specified location.
 Only change the volume of the vessels at the specified location.
 Do not change the volume of the vessels at other locations.
 If the Calculated volume is larger than the Maximum volume, remove liquid from the vessel.
 If the Calculated volume is smaller than the Minimal volume, add liquid to the vessel.
 If there is an air bubble in the liquid, the optical sensor cannot perform a measurement. Remove the air
bubble. To repeat the detection, select the Repeat scan button.
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5.2.7.2 Entering the filling volume manually
If the Input volumes manually function is active, you have to enter the filling volume manually after the start.
Abb. 5-8: Entering the filling volume
Fig. 5-8:
Entering the filling volume
Labware info area
Information on the selected labware.
Liquid detection area
Optical sensor settings for the selected labware.
Off The filling volume will not be checked.
Random positions The filling volume of the first, last and 8
randomly selected positions will be checked.
All used positions The filling volume in all positions used will be
checked.
Tab. 5-1:
Volume column
Enter the filling volume for the
selected labware.
Center table
Column
Meaning
Red border
Invalid filling volume entered. Filling volumes that are smaller than the required
volume are invalid. Filling volumes that are greater than the maximum volume of the
labware are invalid.
Index column
Position in the labware.
Name checkbox
Configure the settings of the optical sensor.
MIN column
Minimum filling volume that is required for this run.
VOL [UL] column
Enter the filling volume manually.
MAX column
Maximum filling volume the labware can be filled with.
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Proceed as follows:
 Enter the filling volume in the VOL [UL] column.
 To enter the same filling volume for the entire labware, press the Set all volumes button. Enter the filling
volume.
 Enter the run parameters of the optical sensor in the Liquid detection area, if necessary.
 To define that epBlue shall use the minimum filling volume for a position, press the Use minimal volume
button.
 Press the Run button.
5.3
Starting the application
Prerequisites
• Labware is positioned on the epMotion worktable.
• epMotion is ready for operation.
• The application run was prepared (see p. 24).
 Click on the Run button.
The application starts.
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5.4
Controlling the application
Abb. 5-9: Ongoing application
Fig. 5-9:
Ongoing application
Carry out the application. The epMotion executes
the application until the end.
Executing the application step-by-step. The
epMotion executes the next step and then stops.
Pause. The application stops, but it will not be
aborted.
Procedure area
Commands. The active command is highlighted
blue.
Worktable area
Worktable.
Remaining Runtime area
Status of the application/runtime of the
application/total time for the application.
Log area
Log.
Aborts the application. If you have stopped the
application using the button, the button is
now active.
 Control the application with the buttons from the Control area.
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5.5
Ending the application
When the application run is finished, a message appears.
Abb. 5-10: The application is finished
Abb. 5-11: The application was canceled
Fig. 5-10: The application is finished
Fig. 5-11: The application was canceled
1. To confirm the message press the OK button.
2. In the Application Runner window, press the Next button.
The final window appears. If your epMotion is equipped with a HEPA air filter and UV lamp, you can
activate the HEPA air filter and the UV lamp.
Abb. 5-12: Final window
Fig. 5-12: Final window
3. Activate the HEPA air filter, if necessary.
Prerequisites
• The worktable of the device must be cleared completely before activating the UV lamp.
4. Activate the UV lamp, if necessary.
5. Press the Exit button to end the application.
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6
epBlue Log viewer - Managing application logs
This chapter describes how to view and save logs of applications.
6.1
Displaying protocols
Abb. 6-1: Logs window
Fig. 6-1:
Logs window
Prerequisites
• The application was executed (see p. 30).
• The epBlue start screen is shown.
1. Click on the LogViewer symbol.
Select the folder in which the application is saved.
2. Select the log.
The log is opened.
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6.2
Saving the log as a PDF file
Prerequisites
• A USB storage medium is connected to the EasyCon Tablet.
1. Click on the
symbol.
The print preview opens.
2. Check the layout and set up the page.
3. Save the document as a PDF file.
4. Close the window.
Tab. 6-1:
Symbols in the print preview
Search in the document.
Show the first page.
Print the document (not active)
Show the previous page.
Page setup.
Show the next page.
Scale the document.
Show the last page.
Zoom in the view.
Create a PDF.
Set the zoom factor.
Close the view.
Zoom out the view.
epBlue Application Editor - Creating and editing applications
epBlue™ with EasyCon
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7
epBlue Application Editor - Creating and editing applications
This chapter describes how to create and edit applications in epBlue.
7.1
Overview
Applications are programmed processes in epBlue. An application consists of equipping the worktable and
a sequence of commands. Each command triggers a specific action.
Applications are created in a fixed order:
1. Create an empty application (see p. 36).
2. Position labware and define the application parameters of the labware (see p. 37).
3. Define the procedure (see p. 43).
4. Check the application (see p. 45).
7.2
Creating an application
7.2.1
Menu bar
Abb. 7-1: Application Editor menu bar
Fig. 7-1:
Application Editor menu bar
Saving the application.
Deleting the application.
Saving the application for another worktable.
One step back.
Saving all applications.
One step forward.
CSV
Closing the application.
Import CSV file.
Cutting the application.
Printing the application.
Copying the application.
Checking the application.
Inserting the application.
Go to Application Runner.
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7.2.2
Creating an empty application
Abb. 7-2: Start screen of the Application Editor
Fig. 7-2:
Start screen of the Application Editor
Prerequisites
• The Application Editor is open.
1. Select the folder in which the application is saved.
2. Click on the
symbol in the menu bar.
The Create new application window appears. epBlue detects the connected device.
3. Enter the name and description of the application.
4. Close the window using the Create button.
You have created an empty application. The Worktable window of the application opens.
7.2.3
Copying applications
You can create new applications by copying and changing existing applications.
The Eppendorf folder contains applications defined by Eppendorf AG. These applications
cannot be executed or changed. They are used as templates and examples.
 In order to use applications from the Eppendorf folder, copy the corresponding application
to your folder.
 To copy an application (see p. 84).
7.2.4
Saving applications
 To save an application, click on the
symbol in the toolbar.
 To save an application under a new name, click on the
 To save all open applications, click on the
symbol in the toolbar.
symbol in the menu bar.
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7.2.5
Positioning labware
7.2.5.1 Positioning the labware on the epBlue worktable
Labware can only be positioned at specific locations. Information on positioning the labware can be found
in the hardware operating manual.
Abb. 7-3: Positioning the labware on the epBlue worktable
Fig. 7-3:
Positioning the labware on the epBlue worktable
Labware Category column
Overview of all labware types.
Worktable area
epBlue worktable with positioned labware.
Labware area
Labware that can be positioned on the epBlue
worktable.
Properties area
Properties of the selected labware.
Switch to procedure button
To go to the Procedure window, press the Switch Placed Labware column
to procedure button.
Labware positioned on the epBlue worktable.
Trash
Delete labware from the epBlue worktable.
Prerequisites
• Labware combinations have been created for reaction vessels (see p. 92).
• The application is open.
• The Worktable window is open.
1. Select labware from the Labware area.
2. Draw the labware to the desired position of the epBlue worktable.
If the labware cannot be positioned at this location, the location is marked in red.
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7.2.5.2 Deleting positioned labware from the epBlue worktable
There are several options to delete labware from the epBlue worktable.
Selecting the labware
 Activate the checkbox for the corresponding labware in the Placed labware column.
 Alternatively, select the labware with the mouse.
Deleting labware
 Drag the selected labware into the trash with the mouse.
 Alternatively, click on the
symbol in the menu bar.
 Alternatively, select the Delete function in the context menu.
7.2.5.3 Stacking labware
The gripper stacks and transports labware. The gripper is required to stack labware or to unstack a stack
during an ongoing application.
You can stack a maximum of 5 plates on the worktable. You can stack a plate on a height adapter. The
maximum stacking height is 126 mm. Stacked labware must have the same properties, e.g. name,
geometry, and bottom tolerance.
The gripper only transports one part at a time. The gripper can stack labware or unstack a stack of labware.
Liquids cannot be transferred in a plate stack.
Functions of the optical sensor with stacked plates
• The Check labware placement function is available.
• The Detect volumes function is not available.
You can stack the following plates:
• EP_pDNA_96_MTP
• EP_TT_PCR_150
• EP_TT_PCR_40
• EP_DWP_1200
• EP_pDNA_96_DWP
• EP_DWP_200
• EP_DWP_2000
• EP_DWP_1000
• EP_DWP_500
• EP_MTP_384
• EP_MTP_96
• EP_MTP_UV_VIS_96
• EP_MTP_CC_96
• EP_MTP_CI_96
• EP_MTP_CC_6
• EP_MTP_CC_12
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•
•
•
•
•
EP_MTP_CC_24
EP_MTP_CC_48
EP_TT_PCR_150
EP_TT_PCR_250
EP_TT_PCR_40
The list is extended if new labware is available.
Plates that are stacked may only be filled up to the working volume.
Abb. 7-4: Stacking labware on the epBlue worktable
Fig. 7-4:
Stacking labware on the epBlue worktable
Proceed as follows to stack labware at a location.
1. Place the lower labware at one location.
2. Position the upper labware at the same location.
The Placed labware column shows, which labware is placed in the epBlue worktable locations.
The number behind the designation shows how many parts have been stacked at this location. Example:
pcr384_3. Only 3 plates have been stacked.
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7.2.6
Defining the application parameters of the labware
You can determine the properties of each labware used.
Abb. 7-5: Properties window
Fig. 7-5:
Properties window
 To change the properties of a labware, double-click on the labware.
 Alternatively, select the labware and go to the Properties context menu with the right mouse button or
click on the Properties button.
The Properties window opens.
 Set the properties for the selected labware.
7.2.6.1 Defining the filling volume of the labware
1. Set how the filling volume of the labware is to be measured.
2. To measure the filling volume with the optical sensor (see Optical sensor on p. 77)
3. To enter the filling volume manually (see Entering the filling volume manually on p. 29)
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7.2.6.2 Opening sealed labware
The epMotion can open the sealing of individual positions of a labware. The epMotion opens the sealing
with a single-channel dispensing tool.
If the Pierce options option is activated, epBlue can open the sealing of the labware with the following
commands:
• Commands Sample transfer, Reagent transfer, Dilute, Pool, Pool one destination: The sealing is opened.
Immediately afterwards, a liquid transfer is performed.
• Pierce command: The sealing is opened. Immediately afterwards, a liquid transfer is performed.
To pierce the sealing there are 2 modes.
• Single pierce mode: The pipette tip on the dispensing tool pierces the aluminum foil once in the middle
of the position.
• Triple pierce mode: The pipette tip on the dispensing tool pierces the aluminum foil three times. The
holes have a fixed distance to the middle of the position. The holes are pierced into the aluminum foil at
a distance of 120°. The Triple pierce mode is used to pierce a big hole into the sealing with small
dispensing tools.
The single-channel dispensing tool TS 1000 opens the sealing in factory setting in the Single pierce mode.
The single-channel dispensing tools TS 50 and TS 300 open the sealing in factory setting in the Triple
pierce mode.
You can change the mode in which the dispensing tool opens the sealing. For this purpose, change the
value in the Distance (see Fig. 7-6 on p. 42) field.
Example: The single-channel dispensing tool TS 1000 opens the sealing in factory setting in the Single
pierce mode. The distance of the dispensing tool to the middle of the position is zero. The value in the
Distance field is zero. You change the value in the Distance field to 1. The single-channel dispensing tool
TS 1000 will open the sealing in the Triple pierce mode. The holes have a distance of 1 mm to the middle of
the position.
After the sealing has been opened, the pipette tips can still be used or ejected (see Pierce command on
p. 62).
If the labware is sealed, the optical sensor cannot measure the filling level. If the Pierce settings option is
activated, the Levelsensor settings option is not available.
The labware must be sealed with aluminum foil that is marked with the word "Pierceable".
If you have any questions on the suitable aluminum foil, please contact Automations Support at Eppendorf.
Write an e-mail to [email protected]
The parameters in the Pierce settings area have been optimized for the labware combination Thermoblock
PCR 96 OC with twin.tec PCR Plate 96, semiskirted.
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Setting the parameters for opening sealed labware
Abb. 7-6: Properties window in the Pierce settings area
Fig. 7-6:
Properties window in the Pierce settings area
Distance
Distance from the middle of the vessel.
If the distance is zero, the single-channel
dispensing tool opens the sealing in the Single
pierce mode.
If the distance is > zero, the single-channel
dispensing tool opens the sealing in the Triple
pierce mode.
Above
Distance between the starting position of the
piercing movement and the vessel edge. At this
distance, the pipette tip starts piercing.
SPEED
Speed at which the pipette tip opens the sealed
labware.
Depth
Distance between vessel edge and pipette tip.
This is how far the pipette tip is immersed into
the vessel.
1. Select the labware.
2. Call up the properties of the labware.
The Properties window opens.
3. Set parameters in the Pierce settings area.
4. Place the labware with an adapter on the worktable.
5. Perform a test run. Check whether the parameters in the Pierce settings area are adjusted to labware and
aluminum foil.
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7.2.6.3 Setting the filling level of the labware
You can set the filling level for all positions of the labware.
1. To set the same filling level for all positions of a labware, press the Set all volumes button.
2. To import the filling levels of the labware from a CSV file, press the Import all volumes button.
3. To enter the filling levels of the labware manually, enter the filling levels in the VOL [µL] column.
7.2.7
Defining procedures
A procedure is a defined sequence of commands which epMotion executes during an application.
Commands are described in the Commands chapter (see p. 46).
The first command of each procedure is the Procedure Start command. The Procedure Start command is
created automatically and cannot be deleted. The Procedure Start command shows the epBlue worktable at
the start of the application. The Procedure Start command does not influence the application.
The software does not include any command for taking up pipette tips. The user defines which pipette tips
will be used in a command. The user must provide for the corresponding pipette tips on the worktable.
The user defines the dispensing tool for each command. Tools are picked up according to the command
parameters.
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Abb. 7-7: Creating a procedure for an application
Fig. 7-7:
Creating a procedure for an application
Commands column
Available commands.
Procedure column
Sequence of commands. The commands are
executed in the order displayed.
Prerequisites
• The application is open.
• Labware is positioned on the epBlue worktable .
• The Procedure window is open.
Worktable
The state of the worktable during the execution of
the selected command
Options area
Parameter of the selected command.
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7.2.7.1 Inserting a command
1. Select a command from the Commands column.
2. Drag the command to the Procedure column.
3. In order to insert a command at the end of the application, double-click on the selected command in the
column Commands.
7.2.7.2 Copying commands
 Select a command from the Procedure column.
 Click on the
symbol in the menu bar.
 Alternatively, open the context menu with the right mouse button and select Copy.
7.2.7.3 Inserting a copied command
1. Copy the command.
2. Select the position in the Procedure column where you would like to insert the copied command.
3. Select the Paste command in the context menu.
The copied command is inserted behind the selected position.
7.2.7.4 Changing the command sequence
 Select a command in the Procedure column.
 Drag the command to the desired position in the procedure.
7.2.7.5 Deleting commands
 Select a command in the Procedure column.
 Delete the command using the symbol in the menu bar or via the context menu.
7.2.7.6 Changing command parameters
1. Select a command in the Procedure column.
The parameters of the selected command are shown in the Options area.
2. Set the parameters to suit your requirements.
7.2.8
Checking an application
1. Click on the
symbol in the menu bar.
2. Select the Check command function.
The application is checked.
Example: Pipette tips without filter are positioned on the epMotion worktable. In the application, pipette
tips with filter were selected. The screen displays an error message.
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7.3
Commands
A procedure is a sequence of commands that epMotion executes during an application run. Commands are
executed one after the other and independent of each other. Each command has a specific set of
parameters.
The software does not include any command for taking up pipette tips. The user defines which pipette tips
will be used in a command. The user positions the corresponding pipette tips on the worktable.
The user defines the dispensing tool for each command. Tools are picked up according to the command
parameters.
The command parameters may differ. The displayed parameters depend on the selected
worktable or on the configuration of the connected epMotion.
Transfer commands
Sample transfer
Reagent transfer
Dilute
Pool
Pool one destination
Additional commands
Mix
ThermoMixer
epMotion with
Eppendorf
ThermoMixer
module
Magnetic separation Temperature
epMotion with
epMotion with
magnetic finger
thermal module
module
Transport
epMotion 5075
epMotion 5073
Exchange
epMotion 5070
Wait
User intervention
Number of samples
External process
Pierce
Comment
epBlue Application Editor - Creating and editing applications
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7.3.1
Sample transfer command
Various transfer commands are available to transfer liquids. All transfer commands are derived from the
Sample transfer command.
Abb. 7-8: Principle of the Sample transfer command
Fig. 7-8:
Principle of the Sample transfer command
The Sample transfer command is used to transfer liquids from various source positions to several
destination positions. The number of samples that will be aspirated from the source labware depends on
the Number of samples (see p. 66) command.
If the Pierce settings function is activated in the properties of the labware, the labware will be opened before
the liquid transfer. If the sealing of a source vessel and the sealing of a destination vessel are to be opened,
the source vessel is opened first. To open the vessels, epBlue uses the same dispensing tool and the same
pipette tips as for liquid transfer.
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7.3.1.1 Sample transfer > Parameter command
Abb. 7-9: Sample transfer > Parameter
Fig. 7-9:
Sample transfer > Parameter
Volume
Select volume in μL or nL. A decimal place is available for volumes ≤ 99.9 μL.
You can transfer a volume larger than the maximum volume of the pipette tip.
The volume to be transferred will be pipetted in several steps.
Example: The user would like to transfer 1500 μL and enters this value into the
Volume field. If a 1000 μL tip is being used, 2x 750 μL will be pipetted. If a
300 μL tip is being used, 5 x 300 μL will be pipetted.
Pipet. tool
Used dispensing tool.
Pipette
The volume is individually aspirated and dispensed for each liquid transfer.
Multidispense
Aspirate liquid and dispense it in the destination position in defined subsets.
Multiaspirate
Aspirate liquid from several source positions using one pipette tip and
dispense the liquid in one destination position. Available for Pool and Pool one
destination commands .
Use filter tips
Activate when using tips with filter.
Source
Select the source labware. The source labware must be placed on the
worktable. When using more than one source labware, the number of positions
in the labware (384, 96, 24) must match. If you dispense within one labware,
the source labware and destination labware are identical.
Destination
Select the destination labware. The destination must be placed on the
worktable. When using more than one destination labware, the number of
positions in the labware (384, 96, 24) must match. If you dispense within one
labware, the source labware and destination labware are identical.
Pattern
Select the source positions and destination positions. Create replicates.
Information on the aspiration and transfer of liquids with the epMotion can be found in the
hardware operating manual.
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7.3.1.2 Sample transfer > Options command
Abb. 7-10: Sample transfer > Options
Fig. 7-10: Sample transfer > Options
Aspirate from bottom
Aspirate liquid from the base of the vessel. When using several vessels with
different volumes, enter the lowest filling volume of these vessels (Sample
transfer > Parameter).
Elution from filter
Aspirate liquids from the supernatant in filter plates. Available if in the
Parameter window the Pipette radio button is selected.
Dispense from top
During liquid dispensing, position the pipette tips 3 mm – 4 mm below the
edge of the vessel. When using several vessels with different volumes, enter
the highest filling volume of these vessels (Sample transfer > Parameter).
Change Tips
Defined time when the pipette tips are changed.
when command finished
Eject pipette tips after the end of the command. The liquid will be aspirated
with the same tip from all source labware positions.
before asp. for next
destination well
Eject the pipette tips before the liquid from the next source position is
aspirated. Liquid from the same source position will be aspirated with the same
tip. Liquid from the next source position will be aspirated using a new tip.
before each aspiration
Eject pipette tips prior to each aspiration. Liquid will always be aspirated with
a new tip. Additional volume will be discarded in the waste container along
with the pipette tip.
Dip tips
After liquid dispensing, a drop may remain on the pipette tip. The drop is
stripped off at the surface of the liquid.
Adjust, how close the pipette tip may approach the surface of the liquid after
liquid dispensing.
The surface of the liquid has the value zero. Positive values indicate the
distance from the surface of the liquid into the liquid. Negative values indicate
the distance from the surface of the liquid to the top.
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keep tips, do not change
tips
Do not eject pipette tips. Liquid will be aspirated with the same tip from all
source positions. The tips are also used in the next command. If the transfer
type changes in one of the following commands, or the Mix command is used,
the tips will be ejected.
after XX aspirations
Pipette tips will be ejected after a defined number of aspirations.
Restore tips
Pipette tips are restored to the position of the box they were taken from.
Is only executed if the tips within an application are reused for the same source
vessel.
The restore tips checkbox is only available with specific settings of the Change
Tip option.
Reuse tips
Restored pipette tips will be reused.
The pipette tips will be reused within an application for the same source vessel.
Is only executed if the restore tips option was selected in a previous command
and the restored tips are available.
The re-use tips checkbox is only available with specific settings of the Change
Tip option.
Information on the aspiration and transfer of liquids can be found in the hardware operating
manual.
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7.3.1.3 Sample transfer > Mix command
The Mix command is also available for mixing (see p. 66).
Abb. 7-11: Sample transfer > Mix
Fig. 7-11: Sample transfer > Mix
Mix before aspirating
Mix before each aspiration. Set the parameters following activation.
If you transfer a larger volume than the maximum volume of the pipette, the
liquid will be mixed before the first aspiration.
Mix after dispensing
Mix after each dispensing. Set the parameters following activation. Available
for Transfer type > Pipette.
If you transfer a larger volume than the maximum volume of the pipette, the
liquid will be mixed after the last aspiration.
No. of Cycles
Number of mixing cycles.
Speed
Mixing frequency. If no entries were made for Speed, the defined aspiration
speed of the liquid type will be used.
Volume
Mixing volume.
Fixed height
Position of the pipette tip on the Z-axis. Fixed aspiration level and dispensing
level. Only for levels below the working volume.
Aspiration
Distance between the pipette tip and base of the vessel during aspiration. If
0 mm is entered, the distance will be corrected to 2 mm. The correction is
dependent on the bottom tolerance and type of labware.
Dispense
Distance between the pipette tip and base of the vessel during dispensing. If
the dispensing position is above the vessel, the distance will be corrected to
the height of the vessel.
Information on mixing liquids can be found in the hardware operating manual.
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7.3.1.4 Sample transfer > Rinse options
Abb. 7-12: Sample transfer > Rinse options
Fig. 7-12: Sample transfer > Rinse options
You can clean used pipette tips using the Sample transfer > Rinse options command. The pipette tips are
rinsed in washing solution after the liquid transfer.
If the re-use tips checkbox is activated in the Sample transfer > Options window, the pipette tips will be
placed on the rack they were taken from after being cleaned. If the checkbox is not activated, the pipette
tips are disposed of after cleaning. You can set the parameters for this with the Sample transfer > Options
command.
Rinse tips before disposal Clean pipette tips.
checkbox
No. of Cycles
Number of cleaning cycles.
Speed
Cleaning speed. If no Speed value is entered, epBlue uses the aspiration speed
of the liquid type.
Volume
Volume of the washing solution that is aspirated into the pipette tip.
Liquid type
Liquid type of the washing solution.
Table Rinse racks
Vessel for washing solution.
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7.3.1.5 Sample transfer > Liquid types
Liquid types are defined in epBlue.
Information on dispensing liquids can be found in the hardware operating manual.
Tab. 7-1:
Defined liquid types in epBlue
Liquid type:
Optimized for
50 µL tips:
50 µL tips:
Comments
Pipetting from Dispensing from
Alcohol 75%
Mixture of
1 to 3 μL
75 % ethanol
and 25 % water
3 μL
• Wash reagent in kits for nucleic
acid purification. See applications.
Alcohol 98%
Alcohol 98 %
1 μL
3 μL
• New pipette tips are pre-wetted
with the liquid to be aspirated.
Prewetting option is set (see p. 55).
• If you are using pipette tips with
300 μL in Multidispense mode and
aspirate a volume of 300 μL, the
gap to the filter is very small. The
filter may be wetted. For 300 μL
pipette tips and an aspiration
volume > 280 μL, use the Pipette
dispensing mode.
Glycerol
Mixture of
1 μL
40 % glycerol
and 60 % water
5 μL
• Liquid type for enzyme solutions.
• If the glycerol content of the
enzyme solutions is significantly
lower, liquid type Water can be
used.
Protein
Water with
1 % albumin
(10 g/L),
0.01 % Triton
X-100
5 μL
• New pipette tips are pre-wetted
with the liquid to be aspirated.
Prewetting option is set (see p. 55).
• The bending of the surface of the
liquid has a negative impact on the
free jet capability of dispensing in
cell culture plates.
5 μL
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Liquid type:
Optimized for
50 µL tips:
50 µL tips:
Comments
Pipetting from Dispensing from
ProteinC
Water with
1 % albumin
(10 g/L),
0.01 % Triton
X-100
5 μL
5 μL
• New pipette tips are pre-wetted
with the liquid to be aspirated.
Prewetting option is set (see p. 55).
• Dispensing the liquid with a
greater distance to the calculated
plane surface of the liquid (4 to 5
mm) than liquid type Protein.
• All other data such as liquid type
Protein
• Recommended for nutrient media.
Rinse
Demineralized
water.
Water with low
surfactant
content.
1 μL
3 μL
• Low aspiration and dispensing
speed, strongly delayed blow-out.
• Other data such as liquid type
Water.
• This liquid type is suitable if the
mixing parameters of a transfer
command or the Mix command are
used.
• Can increase the risk of
contamination for small vessels,
e.g., PCR plates.
• Recommended in combination with
the Mix option for reducing the
residual liquid in the pipette tip.
Speed_xl
Demineralized
water.
1 μL
3 μL
• Mixing at high dispensing speed.
• High dispensing speed poses a
high risk of contamination for small
vessels.
• Good mixing in DWP 96 with
750 μL aspiration and 750 μL
dispensing.
Speed_xs
Demineralized
water.
1 μL
3 μL
• For slow aspiration from filter
plates.
• Very low aspiration speed.
Sediment will not be dispersed.
Water
Demineralized
water.
1 μL
3 μL
• Recommended for most
applications.
• Technical data on the systematic
and random errors were
determined using this liquid type.
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7.3.1.6 Changing the liquid type
Changes to the liquid type have effects on the accuracy of pipetting. Changing the settings
may increase the dispensing deviations.
Abb. 7-13: Sample transfer > Liquid types
Fig. 7-13: Sample transfer > Liquid types
 To change the liquid type, check the Change parameter checkbox.
 Enter the aspiration speed of the liquid in the Aspiration speed field: 0.2 mm/s – 110 mm/s range.
For viscous solutions and large aspiration volumes, select a low aspiration speed. The liquid aspiration
is delayed. The liquid aspiration must be ended before the pipette tip withdraws from the liquid. Select
the low aspiration speed during phase separations or to prevent the distribution of sediments or
particles.
 Enter the dispensing speed of the liquid in the Dispense speed field. Input range: 0.2 mm/s – 110 mm/s
Select a low dispensing speed if you dispense larger volumes in empty vessels. A lower dispensing
speed decreases the risk of liquid spraying back. Use larger vessels to achieve better mixing results due
to a higher speed.
 Enter the blow-out delay in the Blow delay field. Input range: 0 ms – 99999 ms.
The delay is the break between the end of the dispensing during liquid dispensing and blow-out. This
setting has an effect on the Transfer type Pipette.
If you use liquids with high wetting behavior (e.g., solutions which contain proteins or detergents,
organic solvents) and delayed drain-off characteristics (highly viscous liquids), delay the blow-out. The
residual liquid collects in the lower part of the tip prior to the blow-out.
If you use liquids with low wetting behavior (e.g., water, saline solutions), set the blow-out delay to 0.
 Enter the dispensing speed of the blow-out in the Blow speed field. Input range: 0.2 mm/s – 110 mm/s.
Using liquids that tend to form bubbles on the outlet of the pipette tip increases the dispensing speed of
the blow-out. Decrease the dispensing speed if liquid sprays back into the vessel. This setting has an
effect on the Transfer type Pipette.
 In the Blow movement field, enter the path of the piston during blow-out. Input range: 0 % – 100 % as
percentaged value of the setting Initial stroke.
No blow-out will be carried out during when 0 is entered. The entire path of the piston is part of the
dispensing step.
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When entering 100, the entire piston path is available for the blow-out. The blow-out is conducted using
the Blow delay and Blow speed parameters.
Increase and decrease the Blow movement along with the Blow speed setting. This setting only has an
effect on the Transfer type Pipette.
 In the Initial stroke field, enter the path the piston takes before the liquid aspiration. Input range: 0 % –
100 % of the available path, depending on the type of dispensing tool.
The higher the value, the larger the amount of piston path that is available for the blow-out after liquid
dispensing. To decrease the amount of residual liquid in the pipette tip after dispensing, increase the
value. To decrease the formation of foam during dispensing, decrease the value. Reducing the value can
increase the dispensing deviation. The entry 0 means that no blow out will be conducted.
If you change the value, the pipette tips will be replaced before liquid aspiration.
 In the Prewetting field, enter the number of piston movements for pre-wetting the pipette tips. Input
range: 0 – 9 cycles.
Non-wetted pipette tips can be pre-wetted to create the same conditions for dispensing.
Wetting the pipette tips:
• For liquids with low vapor pressure.
• For liquids with reduced surface tension and delayed drain-off characteristics.
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7.3.1.7 Testing amended liquid types on the epMotion
Prerequisites
• The parameters of the liquid types are customized to your requirements.
1. Carry out the sample run of the application with demineralized water.
2. Check the precision and accuracy of the dispensing.
3. Check the working volume of the labware. No liquid may spray out of the labware.
4. Carry out the sample run of the application using the provided liquid.
5. Check the precision and accuracy of the dispensing.
6. Check the working volume of the labware. No liquid may spray out of the labware.
7.3.2
Reagent transfer command
The Reagent transfer command is used to transfer a liquid from one or more source positions into several
destination positions. After the first source position has been emptied, the epMotion switches to the next
source position.
Abb. 7-14: Principle of the Reagent transfer command
Fig. 7-14: Principle of the Reagent transfer command
When using multiple source positions and the optical sensor is switched on , the volume of the first source
position will be checked when starting the application run. If there is not enough liquid available for the
number of samples, the Checkrun window opens. To incorporate the next source position in the calculation,
select accept level and continue. The optical sensor checks the next source position. The calculated volumes
will be summed. The application will start if the volume is sufficient.
The Number of samples command is used to define the number of destination vessels.
The parameters of the command Reagent transfer are identical to the command Sample transfer (see p. 47).
If the Pierce settings function is activated in the properties of the labware, the labware will be opened before
the liquid transfer. If the sealing of a source vessel and the sealing of a destination vessel are to be opened,
the source vessel is opened first. To open the vessels, epBlue uses the same dispensing tool and the same
pipette tips as for liquid transfer.
Proceed as follows to define the pattern for the Reagent transfer command:
1. Select the source position in the source labware. If you are using several source positions, select these
vessels one after the other.
2. Select the destination position in the destination labware.
Source position and destination position for one pattern are the same color.
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7.3.3
Dilute command
The Dilute command is used to create serial dilution series, e.g., for PCR standards. A defined volume is
pipetted several times from one vessel to the next.
Abb. 7-15: Principle of the Dilute command
Undiluted Sample
Diluent
Diluent
Diluent
1. Dilution
2. Dilution
3. Dilution
Fig. 7-15: Principle of the Dilute command
The parameters of the command Dilute are identical to the command Sample transfer (see p. 47).
If the Dilute command is carried out within one labware the source positions and destination positions must
not overlap.
If the Pierce settings function is activated in the properties of the labware, the labware will be opened before
the liquid transfer. If the sealing of a source vessel and the sealing of a destination vessel are to be opened,
the source vessel is opened first. To open the vessels, epBlue uses the same dispensing tool and the same
pipette tips as for liquid transfer.
1. Fill the destination positions with a diluent. Use the Reagent transfer command.
2. Define the number of samples to be diluted Using the Number of samples command.
3. In the pattern, define the number of dilution steps. The pattern is limited by a series or column.
4. Enter the options for the Dilute command.
5. To achieve a good mixture of sample and diluent, activate the Mix > Mix after dispensing option.
The sample and diluent will be mixed after each dispensing.
6. To mix the undiluted sample before the first aspiration, activate the Mix before aspirating option.
Proceed as follows to enter the pattern for the Dilute command:
1. Select the source position of the undiluted sample.
2. Select the destination positions of the dilution steps. The number of positions defines the number of
dilution steps.
3. Repeat the procedure for each source position.
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7.3.3.1 Example of a dilution series
This example explains the concept of a dilution series. The example does not describe the full application.
Objective
• 24 samples are in a rack with 24 positions and should be diluted 1:1000.
• The dilution should be completed in 3 program steps with dilutions of 1:10.
• First, a single-channel dispensing tool is used. An eight-channel dispensing tool will then be used to
reach a higher speed.
Procedure
• 200 μL of each of the samples will be transferred to the 96-well plate using the Sample transfer
command. The dilutions are carried out in this plate.
• 225 μL of the diluent will be transferred from the 300 mL reservoir to each of the empty wells of the
96-well plate using the Reagent transfer command.
• 25 μL of the sample will be aspirated from column 1 using the Dilute command and will be mixed with
respectively 225 μL of the diluent in the columns 2, 3 and 4. The mixing process will be repeated twice
in the following columns.
In this example, each dilution step is a 1:10 dilution. The required 1:1000 dilution will be achieved with the
third 1:10 dilution. The volume aspirated from the undiluted sample also applies for the dilution steps.
Creating dilution series
Transferring undiluted samples to the destination labware
1. Pattern of the Sample transfer command:
The source position and destination position are the same color. epBlue detects the pattern and
suggests the next field.
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Transferring diluent to the destination labware
2. Pattern of the Reagent transfer command:
With an eight-channel dispensing tool, the Reagent transfer command is used to aspirate the diluent
from the 300 mL reservoir and dispense it into the empty wells of the 96-well plate.
Carrying out the dilution
3. Pattern of the Dilute command:
Using an eight-channel dispensing tool, liquid is removed from column 1 and mixed with the diluent in
columns 2, 3 and 4. This procedure will be repeated in the following columns.
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7.3.4
Pool command
The Pool command can be used to combine liquids from several source positions in one or more destination
positions.
Abb. 7-16: Principle of the Pool command
Fig. 7-16: Principle of the Pool command
The parameters of the command Pool are identical to the command Sample transfer (see p. 47).
If you are using the Multiaspirate function, liquid will be removed from the source positions until the
pipette tip is filled. The contents will then be dispensed in the destination position. In the pattern, you
define which source positions will be pooled in the destination position.
The Number of samples command before the Pool command defines the number of source positions.
Options > Change Tips is set to before asp. for next destination well.
If the Pierce settings function is activated in the properties of the labware, the labware will be opened before
the liquid transfer. If the sealing of a source vessel and the sealing of a destination vessel are to be opened,
the source vessel is opened first. To open the vessels, epBlue uses the same dispensing tool and the same
pipette tips as for liquid transfer.
7.3.5
Pool one destination command
The Pool one destination command can be used to combine liquids from several source positions in one
destination position. This command is a simplified Pool command.
The parameters of the command Pool one destination are identical to the command Sample transfer (see
p. 47).
The Number of samples command before the Pool one destination command defines the number of source
positions.
The Options > Change Tips option is set to when command finished. The pipette tips are ejected at the end of
the command.
If the Pierce settings function is activated in the properties of the labware, the labware will be opened before
the liquid transfer. If the sealing of a source vessel and the sealing of a destination vessel are to be opened,
the source vessel is opened first. To open the vessels, epBlue uses the same dispensing tool and the same
pipette tips as for liquid transfer.
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7.3.6
Pierce command
You can open the sealing of individual positions of a labware. The labware must be sealed with suitable
aluminum foil. The epMotion opens the sealing with a single-channel dispensing tool.
You can open the sealing of individual positions using the Pierce command. A liquid transfer does not have
to be performed directly after the Pierce command.
epBlue saves, on which positions of the labware the sealing was opened. This way, the sealing of each
position of the labware can only be opened once.
You have to activate the Pierce settings function in the properties of the labware to open the sealing with the
Pierce command. You have to adapt the parameters of the Pierce settings function to the labware
combination and the aluminum foil .
Abb. 7-17: Pierce > Options command
Fig. 7-17: Pierce > Options command
Pipet. tool checkbox
Select dispensing tool
Racks checkbox
Select Labware
Pattern button
Select Pattern mode to open the labware
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Abb. 7-18: Pierce > Parameter command
Fig. 7-18: Pierce > Parameter command
Change tips radio button
re-use tips checkbox
when command finished Replace pipette tips if all
Restored pipette tips will be reused.
positions have been opened.
The pipette tips will be reused within an
after each perforation Replace pipette tips if a
application for the same source vessel.
position has been opened.
Is only executed if the restore tips option was
keep tips Do not replace pipette tips.
selected in a previous command and the restored
tips are available.
restore tips checkbox
The re-use tips checkbox is only available with
Pipette tips are restored to the position of the box
specific settings of the Change Tip option.
they were taken from.
Is only executed if the tips within an application
are reused for the same source vessel.
The restore tips checkbox is only available with
specific settings of the Change Tip option.
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7.3.7
Comment command
Abb. 7-19: Comment command
Fig. 7-19: Comment command
Comment field
Enter comment
A comment can be output using the Comment command. The comment will be shown when the command
is executed.
7.3.8
Exchange command
The command Exchange is only available for epMotion 5070.
Abb. 7-20: Exchange command
Fig. 7-20: Exchange command
Labware drop-down menu
Labware taken out of the epMotion
Exchange with drop-down menu
Labware to be placed inside the epMotion
The Exchange command can be used to exchange labware. When the command is executed, epBlue
prompts you to replace the labware manually.
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7.3.9
Magnetic separation command
The Magnetic separation command only is available for devices with magnetic finger module.
The Magnetic separation command can be used to separate magnetic beads using the magnetic finger
module.
The Magnetic separation command can only be executed if the PrepRack is positioned on the Eppendorf
ThermoMixer module of the epBlue worktable.
The efficiency of the magnetic separation depends on the properties of the used beads. The Duration
should be at least 2 min.
If the Magnetic separation command is executed when the Separation On checkbox is checked, the
magnetic fingers will be drawn out. During the waiting period, the magnetic beads will be separated by the
drawn out magnetic fingers on the tube inner wall. After the waiting period has elapsed, the next command
in the procedure will be executed. If the Magnetic separation command is executed when the Separation On
checkbox is not checked, the magnetic fingers will be drawn in.
Abb. 7-21: Magnetic separation command
Fig. 7-21: Magnetic separation command
Separation enabled checkbox
Check the checkbox to start the separation
Uncheck the checkbox to end the separation
Duration field
Waiting period. The application stops until the
waiting period has elapsed.
Starting the separation
 To start the separation, execute the Magnetic separation command when the Separation On checkbox is
activated.
The magnetic fingers will be drawn out.
Exiting the separation
 To end the separation, execute the Magnetic separation command when the Separation On checkbox is
deactivated.
The magnetic fingers are drawn in.
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7.3.10
Mix command
The Mix command can be used to mix liquids in a position by multiple aspiration and dispensing steps.
The options of the Mix command are identical with the options of the Sample transfer command in the Mix
area (see p. 51).
7.3.11
Number of samples command
The Number of samples command is used to define how many samples or positions will be processed in the
following transfer commands. The Number of samples command is valid until it has been replaced by a new
Number of samples command.
You can use several Number of samples commands within one application. When the application starts, the
software will query entries for the commands Number of samples. If you do not want to execute part of the
application, enter 0.
If you do not use the Number of samples command, epBlue asks you for the number of samples at the start
of the application. This entry then applies for all commands of the application.
The number of samples is limited by the pattern.
Abb. 7-22: Number of Samples command
Fig. 7-22: Number of Samples command
Fix Sample Count checkbox
Check the checkbox in order to define the
number of samples
Uncheck the checkbox to enter the number of
samples at the start of the application.
Number of Samples field
Active if the Fix number of samples checkbox is
checked. Enter the number of samples. This value
will be taken into account for the pattern.
Max Number of Samples field
Active if the Fix number of samples checkbox is
not checked. Maximum number of samples that
can be processed in the application.
Comment field
This text field is displayed when the software asks
for the number of samples. Enter explanatory
information in the field.
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In accordance with the subsequent command, the Number of samples command has the following
effects:
• Prior to the Sample transfer command: Number of samples aspirated from the source labware.
• Prior to the Reagent transfer command: Number of destination positions liquid is dispensed into.
• Prior to the Pool or Pool one destination command: Number of source positions from which liquid is
removed.
• Prior to the Dilute command: Number of samples to be diluted. The number of dilution steps is defined
by the pattern.
• Prior to the Mix command: Number of source positions in which liquid is mixed.
• With an eight-channel dispensing tool: 8 samples are processed for values 1 – 8. For values 9 - 16, 16
samples are processed, etc.
7.3.12
Temperature command
The command Temperature only is available for devices with thermal module.
With the Temperature command, you can temper the labware in a thermal module. The temperature control
stops when epBlue is exited.
Abb. 7-23: Temperature window
Fig. 7-23: Temperature window
Location drop-down list
Select a thermal module.
Keep Temperature after application run checkbox
Activate the thermal module for the period after
the application has exited. This function can be
used to cool samples after the application run has
been completed.
Temperature on checkbox
Activate the thermal module. Activated thermal
modules are switched off when you uncheck the
checkbox.
Temperature field
Set the set temperature of the thermal module.
0 °C – 110 °C range.
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7.3.13
ThermoMixer command
The ThermoMixer command is only available for devices with Eppendorf ThermoMixer
module.
The ThermoMixer command can be used to set the temperature and speed of the Eppendorf ThermoMixer
module.
Abb. 7-24: ThermoMixer window
Fig. 7-24: ThermoMixer window
Template drop-down list
The labware library contains templates for the
Eppendorf ThermoMixer module.
Temperature on checkbox
Activate the tempering of the Eppendorf
ThermoMixer module.
Mixing on checkbox
Activate the mixing process of the Eppendorf
ThermoMixer module.
Keep temperature after applicaton run field
Activate the temperature control for the period
after the end of the application. This function can
be used to cool samples or reagents after the end
of the application.
Speed field
Set the mixing frequency. 300 rpm – 2000 rpm
range.
Duration field
Set the mixing time. 5 s – 120 min range.
Temperature field
Set the set temperature of the Eppendorf
ThermoMixer module, 4 °C – 95 °C range.
Information on the Eppendorf ThermoMixer module can be found in the epMotion hardware
operating manual.
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NOTICE! Contamination of samples and the device due to excessive speeds.
If the speed is set too high, liquid sprays out of the vessels. Labware may become loose from
the holder and fly around.
 Observe the labware maximum speeds.
Tab. 7-2:
Maximum speed for labware on the Eppendorf ThermoMixer module
Labware
Maximum speed in rpm
Vessels
1000
Deepwell plates with 96 wells
1200
Deepwell plates with 384 wells
1200
Microplate with 6 wells
1000
Microplates with 96 wells
2000
Microplates with 384 wells
2000
PCR plate with 96 wells
2000
PCR plate with 384 wells
2000
ReservoirRack
Mixing not possible
Thermorack or Thermoblock
1000
Thermoblock 96 with a semi-skirted PCR plate for 250 μL
1700
Thermoadapter for Microplate 96/U/V with Eppendorf Microplate 96/V
1600
Thermoadapter for Microplate 96/U/V with Eppendorf Microplate 96/U
1600
Thermoblock PCR 96 OC
1700
Thermoadapter for 96 wells
1000
Thermoadapter for 384 wells
1000
Thermorack TMX
1300
PrepRack
1300
7.3.13.1 Creating a template for the Eppendorf ThermoMixer module
Prerequisites
• The ThermoMixer command is selected (see p. 68).
1. Edit the template in the Options field.
The word Changed appears next to the Save button.
2. Enter the name of the template.
3. Click on the Save button.
The template is saved.
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7.3.13.2 Managing templates for the Eppendorf ThermoMixer module
Abb. 7-25: Labware Editor > TMX Templates
Fig. 7-25: Labware Editor > TMX Templates
 Manage the templates for the Eppendorf ThermoMixer module in the Labware Editor > TMX Templates
folder.
7.3.14
Transport command
The command Transport only is available for devices with gripper.
The Transport command can be used to transport labware. The gripper takes labware from one location and
transports it to another location.
Abb. 7-26: Transport window
Fig. 7-26: Transport window
Labware
Select the labware.
To location
Select a location.
Information on the labware that is being transported can be found in the hardware operating
manual.
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7.3.15
User intervention command
The User intervention command is used to interrupt an application. The application will be continued when
the user confirms a message.
You can use the User intervention command to empty the waste container manually.
Do not change the filling volume during the interruption. If you need to change the filling
volume, use several applications to do so.
Do not change the equipping of the worktables.
Abb. 7-27: User Invention window
Fig. 7-27: User Invention window
Comment window
The comment appears when the command is
executed.
Alarm checkbox
An acoustic signal sounds if the command is
executed.
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7.3.16
External process command
With the External process command, labware can be removed from the device. The labware can be further
processed outside of the epMotion. The application waits until the labware is reset.
Abb. 7-28: External process command
Fig. 7-28: External process command
Labware drop-down list
Select labware to be removed.
Rescan location checkbox
The optical sensor checks if the reset labware is
positioned correctly.
Comment field
Enter comments
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7.3.17
Wait command
The Wait command is used to create a waiting period. The application will be continued after the waiting
period. The command can be used to take into account incubation periods between the addition of
reagents.
Abb. 7-29: Wait window
Fig. 7-29: Wait window
Duration field
Waiting period.
Wait for temperature checkbox
The waiting period begins after the thermal
module has reached the set temperature.
7.4
Location drop-down list
Thermal module whose temperature control is
waited for.
Pattern
For the pattern, enter the source position and destination position between which liquid transfers take
place.
Type of pattern
Description
Regular pattern
•
•
•
•
Available for all commands.
With regular pattern.
epBlue detects and completes the pattern.
Not available for ReservoirRacks and ReservoirRack modules.
Irregular pattern
•
•
•
•
Available for some commands.
Pattern according to a random pattern.
epBlue cannot detect and complete the pattern.
Replicates not possible.
Whether you can define regular or irregular patterns depends on the command and the labware.
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Tab. 7-3:
Pattern options with regular labware
Command
Source labware pattern
Destination labware pattern
Sample transfer
Regular and irregular
Regular and irregular
Reagent transfer
Regular and irregular
Regular and irregular
Pool
Regular
Regular and irregular
Pool one destination
Regular and irregular
Regular and irregular
Dilute
Regular and irregular
Regular
Mix
Regular and irregular
Regular and irregular
Tab. 7-4:
Pattern options with a ReservoirRack
Equipped with ReservoirRack module for
Pattern
ReservoirRack
Reservoir 30 mL
Reservoir 100 mL
Regular
ReservoirRack
Individual vessels
Irregular
7.4.1
Menu bar in the Edit Pattern window
Abb. 7-30: Menu bar in the Edit Pattern window
Fig. 7-30: Menu bar in the Edit Pattern window
Reset
Reject pattern.
Back arrow
One step back.
Complete
Regular patterns are completed automatically.
The pattern is ended when the number of
samples defined in the Number of Samples
command is reached.
Preview
Preview of the pattern with an increased number
of samples.
Show
Show the pipetting sequence.
Next arrow
One step forward.
7.4.2
Defining regular patterns
Prerequisites
• Labware is positioned on the worktable.
• Transfer command or Mix command activated.
1. Select the source labware in the Source area.
2. Select the destination labware in the Destination area.
3. Click the Pattern button.
The Edit Pattern window appears.
4. To delete an existing pattern, press the Reset button.
If you work with an eight-channel dispensing tool, select the top position. For a 96-well pate,
this is row A. For a 384-well plate, this is rows A or B. epBlue adds the pattern for the
remaining channels.
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5. Select the first source position in the source labware.
6. Select the first destination position in the destination labware. To create replicates, select several
destination positions one after the other.
Abb. 7-31: Selecting the first source position
Abb. 7-32: Selecting the first destination position
Fig. 7-31: Selecting the first source position
Fig. 7-32: Selecting the first destination position
7. Select the next source position in the source labware.
8. Select the next destination position in the destination labware.
Abb. 7-33: Selecting the next source position
Fig. 7-33: Selecting the next source position
Abb. 7-34: Selecting the next destination position
Fig. 7-34: Selecting the next destination position
epBlue attempts to detect the pattern and marks the next position gray.
If you created replicates during the first step, and select the destination position for the second liquid
transfer, the positions are defined for the replicates in the same order as in the first step.
9. If the pattern matches your requirements, press the Complete button.
The pattern is completed automatically up to the defined number of samples.
If you would like to discard the pattern, press the Reset button.
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10. To check a pattern, press the Show button.
The order of the pattern is displayed. The source position and destination position of a liquid transfer
are the same color.
7.4.3
Defining irregular patterns
Irregular patterns can be defined for source labware and destination labware. If the pattern is irregular, it is
not completed.
Prerequisites
• Labware is positioned on the worktable .
• Create the application with the Transfer or Mix command (see p. 47).
1. Click the Pattern button.
Abb. 7-35: Edit Pattern window
Fig. 7-35: Edit Pattern window
The Edit Pattern window appears.
2. Check the Source checkbox in the Irregular pattern area.
3. Optional in the Destination area: check the Irregular pattern checkbox.
4. Define the pattern. Specify all source positions and destination positions.
If the pattern is irregular, the software does not complete the pattern automatically. The Complete
button is not active.
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7.5
Optical sensor
The principle of operation of the optical sensor is described in the hardware manual.
The optical sensor has the following functions:
Measure fill level in vessels
• The optical sensor determines the filling level of the labware positions. There are 2 ways of doing this:
– The optical sensor determines the filling level in all positions used.
– The optical sensor determines the filling level in the first position, in the last position and in 8
randomly determined positions.
– The optical sensor cannot measure the fill level in the following labware: 384-well plates, Universal
Disc from Focus Diagnostics, Qiagen rotor disc 72, 100.
Detect pipette tips
• The optical sensor determines the quantity and the positions of the ep.T.I.P.S. pipette tips. Motion
pipette tips in TipHolders and Racks by Eppendorf.
Check labware on the worktable
• The optical sensor scans the code on the labware.
• epBlue checks if the labware on the epMotion worktable matches the labware on the epBlue worktable.
If the optical sensor is switched off, the processing time of the application is reduced.
7.5.1
Measuring the filling level of the labware
The Detect volumes function enables you to measure the filling volume in the labware positions
The optical sensor detects the filling volume up to the detection limit. The detection limit depends on the
labware geometry. Generally, levels of 3 mm or higher can be detected. Each labware definition contains
information on the detection limit of the filling volume. If the filling volume in one position is below the
detection limit, you have to enter the filling volume manually.
The optical sensor can execute the Detect volumes function based on different parameters. You define these
parameters as application parameters when you create an application. When you start a run, you can
change the application parameters. You can define individual run parameters for each run.
Application parameters
Run parameter
Defined in the Application Editor.
Defined in the Application Runner.
Defined when creating the application (see p. 78)
Defined when starting the run (see p. 80).
Defined individually for each labware used in the
application.
Applies for the complete labware of the application.
Different parameters can be defined for each run.
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7.5.1.1 Defining application parameters for measuring the filling volume of plates
Abb. 7-36: Properties window for plates
Fig. 7-36: Properties window for plates
Tab. 7-5:
Parameters of the Detect volumes function
Off
• Deactivate the optical sensor.
• The filling volumes of all positions must be entered when the application is started.
For labware with ≤ 56 positions, the filling volume for each position can be specified in
the Volume column.
For labware with > 56 positions, one filling volume can be specified for all positions.
This filling volume is recommended at the start of the application, and can be changed.
• The volume specification does not take into account the surface meniscus of the liquid.
• If the filling volume is not enough, the result may be faulty dispensing.
All positions
• Checks the filling volume in all positions used in the application.
• If you work with a single-channel dispensing tool, epBlue uses the calculated filling
volume for each position.
• If you work with a multi-channel dispensing tool, the epBlue uses the lowest calculated
filling volume in a column for liquid aspiration and the highest calculated volume in a
column for liquid dispensing.
Random
positions
• The optical sensor checks the filling volume of the first, last and 8 randomly selected
positions.
• For the parameter to be available, the labware has to have at least 24 positions.
If the filling volumes in one plate differ significantly or the labware has very low filling
volumes, use the Aspirate from bottom and Dispense from top options.
 To set the application parameters for the Detect volumes function (see To change the properties of a
labware, double-click on the labware. on p. 40).
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7.5.1.2 Application parameters for measuring filling volume of special labware
Abb. 7-37: Properties window for special labware
Fig. 7-37: Properties window for special labware
The optical sensor cannot measure the filling level in the following labware:
• 384-well plates
• Universal Disc by Focus Diagnostics
• Qiagen Rotor-Disc 72 and Qiagen Rotor-Disc 100
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7.5.1.3 Defining the run parameters for measuring the filling volume
Abb. 7-38: Run parameters when starting the application
Fig. 7-38: Run parameters when starting the application
Detect volumes radio button
Input volumes manually radio button
The optical sensor measures the filling volume.
Enter the filling volume manually.
In which positions the optical sensor measures
Detect tips slider
the filling volume is defined in the labware
Check-in which positions pipette tips are located
properties.
in the labware.
The settings made in the labware properties are
overwritten with the run parameters.
Check labware placement slider
Check the labware on the worktable
Use required minimum volume radio button
epBlue assumes that all vessels are filled with the
minimum required volume for this run.
 To set the run parameters for the Detect volumes function (see Setting the run parameters for the optical
sensor on p. 26).
Examples:
Prerequisites
• Application parameters Off
• Run parameter Detect levels
 The filling volume is measured at the start of the application.
Prerequisites
• Application parameters All positions
• Run parameter Input level manually
 The filling volume must be entered manually when the application is started.
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7.5.2
Detecting pipette tips
You can only use the Detect tips function, if you are using epT.I.P.S. Motion in TipHolders and racks by
Eppendorf.
Enter the Detect tips function as a run parameter at the start of the application (see Setting the run
parameters for the optical sensor on p. 26).
The Detect tips function checks the type of pipette tips and determines the number and position of tips in
the rack.
The epT.I.P.S. Motion tray is coded. The optical sensor uses the code to detect the type of pipette tips.
Arrangement of pipette tips if the Detect tips function is activated.
Tab. 7-6:
1
2
3
4
5
6
7
8
9
10
11
1
12
2
3
4
5
6
7
8
9
10
11
1
12
A
A
A
B
B
B
C
C
C
D
D
D
E
E
E
F
F
F
G
G
G
2
3
4
5
6
7
8
9
10
11
12
The Detect tips function checks to see if there are enough pipette tips available for the application. The
pipette tips must be continuously positioned without gaps. epBlue prompts you to refill the pipette tips
after the available tips have been depleted. In the next application, you can continue using a rack that has
already been opened.
Arrangement of pipette tips if the Detect tips function is deactivated.
Tab. 7-7:
1
2
3
4
5
6
7
8
9
10
11
12
1
2
3
4
5
6
7
8
9
10
11
1
12
A
A
A
B
B
B
C
C
C
D
D
D
E
E
E
F
F
F
G
G
G
2
3
4
5
6
7
8
9
10
11
12
If the Detect tips function is deactivated, the columns of the rack must be fully equipped. The tips must be
available as of position A1.
7.5.3
Checking the labware on the worktable
Enter the Check labware placement function as a run parameter when starting the application (see Setting
the run parameters for the optical sensor on p. 26).
The Check labware placement function detects the labware coding. The function checks if the labware on
the epMotion worktable matches the equipment of the epBlue worktable.
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epBlue Application Editor - Managing applications and folders
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8
epBlue Application Editor - Managing applications and folders
This chapter describes how to manage applications in epBlue. Folders are managed according to the same
rules.
8.1
Menu bar and start screen
Abb. 8-1: Application Editor menu bar
Fig. 8-1:
Application Editor menu bar
Creating a new application
Cutting an application or folder
Creating a new folder
Pasting an application or folder
Opening an application
Deleting an application or folder
Editing the application properties
Importing an application
Copying an application or folder
Exporting an application
Abb. 8-2: Start screen in the Application Editor
Fig. 8-2:
Start screen in the Application Editor
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8.2
Copying and deleting applications
8.2.1
Copying applications
You can copy applications.
The Eppendorf folder contains applications defined by Eppendorf AG. These applications are
used as templates and program examples.
 Copy this application into your area in order to modify and execute it.
Prerequisites
• The start screen in the Application Editor is open.
1. Select the desired application.
2. Click on the
symbol in the menu bar.
3. Select the folder in which the application is.
4. Click on the
symbol in the menu bar.
The application appears in the selected folder.
8.2.2
Deleting an application
You can delete an application.
Prerequisites
• The start screen in the Application Editor is open.
1. Select the application.
2. Click on the
symbol in the menu bar.
3. Confirm deleting the application. To do this, press the OK button in the message window.
The application is deleted.
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8.3
Changing the properties and name of the application
You can change the name and properties of an application.
Prerequisites
• The start screen in the Application Editor is open.
 Select the application.
The properties of the application appear in the Properties field.
 Press the
symbol in the menu bar.
The Edit Properties window opens.
Abb. 8-3: Edit Properties window
Fig. 8-3:
Edit Properties window
 Change the application settings.
 To save the changes, press the Save button.
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8.4
Importing and exporting applications
8.4.1
Importing applications
Tab. 8-1:
You can import applications in the following file formats:
File format
Source of file
*.ws or *.lhs
Applications from the control panel
*.dws
Applications from versions < 20.X of epBlue
*.export
Applications from versions ≥ 20.X of epBlue
Prerequisites
• A USB storage medium is connected.
• The start screen in the Application Editor is open.
1. Select the folder into which the application is to be imported.
2. Click on the
symbol in the menu bar.
The Import Applications window opens.
3. Select the applications to be imported.
4. Press the Open button.
The Import Application window opens.
Abb. 8-4: Import Application window
Fig. 8-4:
Import Application window
Name area
List of all applications for which import was
selected.
Standard application checkbox
Activate if the application is to be imported.
Selected checkbox
Activated, if the application is an Eppendorf
template.
Define import rules area
Define rules for the import of the applications.
Materials tab
Show the labware used in the applications.
Import button
Import applications.
Cancel button
Cancel the import.
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5. Define the rules for importing labware.
6. Press the Import button.
The files will be imported. A summary of the import will be displayed.
7. Press the Finish button.
If you import applications from older software versions, the display of the worktable in epBlue
may not match your device configuration. To remedy this problem, save the application under
a new name using Save as.
8.4.2
Exporting applications
You can export applications individually. You can export a folder with applications.
Prerequisites
• A USB storage medium is connected.
• The start screen in the Application Editor is open.
1. Select the application to be exported.
2. Click on the
symbol in the menu bar.
The Browse For Folder window opens.
3. Select the folder in which the application is to be saved.
4. Press the OK button.
The applications are exported.
Exported applications can only be used with epBlue from version 40.4.
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8.5
Saving an application as a PDF file
You can save an application as a PDF file. An application includes the procedure and equipping of the
worktable. You can save an application protocol as a PDF file (see p. 34).
Prerequisites
• A USB storage medium is connected.
• The application is open.
1. Click on the
symbol.
The print preview opens. The menu bar contains the following symbols:
Search in the document.
Show the first page.
Print the document (not active).
Show the previous page.
Page setup.
Show the next page.
Scale the document.
Show the last page.
Zoom in the view.
Create a PDF.
Set the zoom factor.
Close the view.
Zoom out the view.
2. Check the layout and set up the page.
3. Save the document as a PDF file.
4. Close the window.
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8.6
Saving an application for a different epMotion model
You can save an imported application to execute on your epMotion.
You can create applications for a different epMotion model. You can then export these applications and
import them to the software of a different model . Exported applications can only be used with epBlue from
version 40.4.
If you save applications for a specific epMotion model the epBlue worktable of the application will adapt to
the epMotion worktable of the selected device.
Abb. 8-5: Save As window
Fig. 8-5:
Save As window
Filename
Name of the application
Device model
Name of the epMotion for which the application is
saved
Features
Configuration of the epMotion for which the
application is saved
Prerequisites
• The application is open.
1. Click on the
button in the toolbar.
The Save as window opens.
2. Select the folder in which the application is saved.
3. Select the name and configuration of the epMotion for which the application is saved.
4. Enter the name of the application.
5. Press the Save button.
The application has been saved.
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epBlue Labware Editor - Creating and managing labware
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9
epBlue Labware Editor - Creating and managing labware
This chapter describes how to create and manage labware and labware combinations in epBlue.
Information on properties and use of labware can be found in your hardware operating manual.
9.1
Overview of labware
The name of the labware includes information on the manufacturer and type. Example: EP_MTP_384V
stands for Eppendorf_microplate_384 wells in V-Form.
Tab. 9-1:
Labware types
Abbreviation
Description
DWP
Deepwell plate
FP
Filter plate
MTP
Microplate
PCR
PCR plate
TP
Plate with vessels that can be removed individually.
Cleanup
Plate from the PCR Cleanup Kit
DNA/RNA
Plate from the kit for purification and insulation
TT
Eppendorf twin.tec
PCR Plate Thermo
Fixed combination of thermoblock and PCR plate
Quantities
Maximum filling volume of a vessel in mL or μL
Tab. 9-2:
Plates
Labware type
Labware
DWP 96
Deepwell plate with 96 wells
DWP 384
Deepwell plate with 384 wells
MTP 6
Microplate with 6 wells
MTP 24
Microplate with 24 wells
MTP 96
Microplate with 96 wells
MTP 384
Microplate with 384 wells
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9.2
Managing labware
9.2.1
Menu bar
Abb. 9-1: Menu bar in the Labware Editor
Fig. 9-1:
Menu bar in the Labware Editor
Create new labware.
Import labware.
Open labware.
Export labware.
Delete labware.
Export several labware at the same time.
Display labware properties.
Abb. 9-2: Start screen of the Labware Editor
Fig. 9-2:
Start screen of the Labware Editor
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9.2.2
Deactivating and activating labware
To keep a clear overview of your labware library in the Application Editor, you can deactivate labware that is
not being used. Deactivated labware can be reactivated at any time.
Applications with deactivated labware do not start. If you start an application with deactivated labware, an
error message appears.
Proceed as follows:
1. Open the Labware Editor.
2. Select the labware.
The status of the labware is displayed in the Properties area.
3. To deactivate the labware, flip the switch from active to inactive.
4. To activate the labware, flip the switch from inactive to active.
9.2.3
Deleting labware
You can delete labware that you have created. Proceed as follows:
Prerequisites
• The Labware Editor is open.
1. In the Labware Library, select the labware to be deleted.
2. Check to see if the selected labware is activated.
3. Deactivate activated labware (see p. 93).
4. Click on the
9.2.4
symbol to delete the labware.
Importing labware
You can import labware definitions in zip and export formats. Proceed as follows:
Prerequisites
• A USB storage medium is connected.
• The Labware Editor is open.
1. Click on the
symbol in the menu bar.
The Open Labware window opens.
2. Select and highlight the labware.
3. Press the Open button.
The Import labware window opens.
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Abb. 9-3: Import labware window
Fig. 9-3:
Import labware window
Name area
List of the labware selected for import.
Define import rules area
Define rules for the import of the labware.
Standard labware checkbox
Activated, if the labware is standard Eppendorf
labware.
Import button
Import labware.
Selected checkbox
Activate if the labware is to be imported.
Cancel button
Cancel import.
4. Check selected labware definitions. Define rules for the import.
5. To import the labware definitions, click on the Import button.
The labware definitions are imported to epBlue.
Abb. 9-4: Imported labware
Fig. 9-4:
Imported labware
6. End the import by clicking on the Close button.
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9.2.5
Exporting labware
You can export labware definitions. You can export individual labware definitions or the content of a folder.
You can use exported labware definitions for example on another epMotion.
Exported labware can only be used with epBlue from version 40.4.
Proceed as follows:
Prerequisites
• A USB storage medium is connected.
• The Labware Editor is open.
1. Select labware or folders.
2. Click on the
symbol in the menu bar.
Abb. 9-5: Export labware window
Fig. 9-5:
Export labware window
The Export labware window opens.
3. Select the labware you would like to export from the Selected column.
4. Confirm the selection by pressing Save.
The Browse For Folder window opens.
5. Select the folder where you want to save the labware.
6. Start export. Press the OK button.
The labware is exported to the selected folder in the format .export.
9.2.6
Viewing the properties of the labware
Proceed as follows:
Prerequisites
• The Labware Editor is open.
 Select the labware.
The properties of the labware are displayed in the Properties column.
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9.2.7
Setting the bottom tolerance
NOTICE! Material damage due to placing the pipette tip on the base of the vessel.
If the pipette tips touch the bottom of the tube, the bottom tolerance is too low. The labware
becomes damaged and the dispensing volume is distorted.




Test the application without liquid.
Check the bottom tolerance when exchanging pipette tips, plates, and vessels.
If pipette tips become jammed at the base of the vessel, increase the bottom tolerance.
If deviations in the dispensing volume occur, increase the bottom tolerance.
Proceed as follows:
Prerequisites
• The Labware Editor is open.
1. Select the labware.
2. Click on the
symbol.
The Edit Properties window opens.
Abb. 9-6: Edit Properties window
Fig. 9-6:
Edit Properties window
Name field
Name of labware
Remain Volume field
Remaining volume. Is calculated.
Comment field
Description of labware
Eppendorf Standard Labware checkbox
This checkmark is placed automatically and
cannot be removed. Labware definitions from
Eppendorf are write protected.
Bottom Tolerance field
Bottom tolerance. The lowest bottom tolerance is
200 μm.
Save button
Save changes.
3. Change the bottom tolerance in the Bottom Tolerance field.
4. Save changes using the Save button.
The settings are saved. If the labware is write protected, a copy will be saved. Copies are numbered
consecutively.
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9.3
Creating labware combinations
Before you create an application, you have to create the required labware combination and save it in your
labware library.
The following options are available to you:
• Equipping racks, thermoracks, or ReservoirRack modules with individual vessels.
• Equipping ReservoirRack with reservoirs or ReservoirRack modules.
• Create a labware combination based on existing labware.
9.3.1
Creating a labware combination.
Prerequisites
• The Labware Editor is open.
Abb. 9-7: Create new labware window
Fig. 9-7:
Create new labware window
Name field
Name of labware
Type option field
Desired labware combination
Comment field
Description of labware
1. Click the
symbol in the menu bar.
The Create new labware window opens.
2. Enter the name, description, and labware combination.
Racks/thermoracks + tubes
Equip the rack and thermorack with individual vessels.
Modules + tubes
Equip the ReservoirRack module with tubes.
ReservoirRack 7
Equip the ReservoirRack 7 with ReservoirRack modules.
ReservoirRack 3
Equip the ReservoirRack 3 with ReservoirRack modules.
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3. Click the Create button.
The labware combination is opened. You can create the labware combination.
Abb. 9-8: Creating labware
Fig. 9-8:
Creating labware
epBlue Labware Editor - Creating and managing labware
epBlue™ with EasyCon
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9.3.2
Creating a labware combination based on existing labware
Abb. 9-9: Start screen of the Labware Editor
Fig. 9-9:
Start screen of the Labware Editor
Prerequisites
• The Labware Editor is open.
1. Select the labware.
The Properties column displays information about the labware.
2. Click the
symbol in the menu bar.
The Edit Properties window opens.
Abb. 9-10: Edit Properties window
Fig. 9-10: Edit Properties window
3. Edit the properties of the labware.
4. Enter a new name in the Name field.
5. Press the Save button.
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9.3.3
Equipping the racks, thermoracks, and ReservoirRack modules with individual
vessels
Abb. 9-11: Creating a labware combination
Fig. 9-11: Creating a labware combination
Racks column
Racks and ReservoirRack modules. If you have
selected a rack, only vessels that can be
combined with the rack is shown.
Tubes column
Individual vessels. If you have selected an
individual vessel, only racks that can be
combined with an individual vessel is shown.
Properties column
Information on the selected labware
Clear button
Delete the selected individual vessel, rack,
ReservoirRack module.
Symbol
Close the labware.
Symbol
Save the labware combination as new labware.
Symbol
Save changes.
Reset button
Undo changes. Return to the status before the
last save.
Proceed as follows:
Prerequisites
• The labware was created with the Racks and Tubes or Module Rack + Tubes option.
• The labware is open.
1. Select a rack in the Racks column.
2. Select matching vessels in the Tubes column.
epBlue Labware Editor - Creating and managing labware
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Abb. 9-12: Rack and vessel as labware combination
Fig. 9-12: Rack and vessel as labware combination
3. To save the labware combination, click the
symbol in the menu bar.
The labware combination is saved in the labware library.
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9.3.4
Equipping the ReservoirRack with reservoirs and ReservoirRack modules
Abb. 9-13: Equipping ReservoirRack
Fig. 9-13: Equipping ReservoirRack
Available tubs column
Save button
Reservoirs and equipped ReservoirRack modules
Save the changed combination.
Vendor Info field
Properties of the selected labware.
Save as button
Save the new combination.
Proceed as follows:
Prerequisites
• The ReservoirRack modules were created with the Module Rack + Tubes option.
• The labware is open.
1. Select the reservoir or equipped ReservoirRack module from the Available tubs column.
2. Drag the reservoir or equipped ReservoirRack module to the desired position in the ReservoirRack.
The epBlue worktable shows which reservoirs and ReservoirRack modules the ReservoirRack has been
equipped with.
3. To replace a ReservoirRack module, drag the new ReservoirRack module to the desired position.
The old ReservoirRack module is automatically removed.
4. Save the labware combination.
epBlue Labware Editor - Creating and managing labware
epBlue™ with EasyCon
English (EN)
9.4
Labware library
epBlue is delivered with a comprehensive labware library. The labware library contains data on the
geometry of reaction vessels, pipette tips, plates, racks, reservoirs, adapters and blocks. A complete
overview of the contents of the labware library from epBlue can be found on the www.eppendorf.com/
automation webpage.
Eppendorf AG creates labware definitions for labware from other manufacturers. Eppendorf AG does not
regularly check labware from other manufacturers. For example, if a manufacturer changes the dimensions
of a vessel, the labware definition no longer matches the changed vessel.
9.4.1
Loading labware definitions from the Internet
Eppendorf provides labware definitions on the www.eppendorf.com/automation webpage for download.
You can use different criteria to search on the webpage.
To download labware from the webpage, register in the VIP area on the www.eppendorf.com/automation
webpage.
To import the downloaded labware to epBlue (see p. 93).
9.4.2
Request labware definitions
Eppendorf creates labware definitions for labware that you would like to use in the epMotion. You can
record these definitions in the epBlue labware library.
1. Check to see if the definition for your labware is in the epBlue labware library.
2. Check to see if the definition for your labware is in the labware library on the www.eppendorf.com/
automation webpage.
3. If the definition is not available for your labware, send a request to Eppendorf AG.
If Eppendorf AG has a definition for your labware, you will receive the data from Eppendorf AG via
e-mail.
If Eppendorf AG does not have a definition of your labware, you will receive a form with questions on
your labware.
9.4.3
Add a labware definition to a labware library
1. Transfer labware definitions to a USB storage medium.
2. Insert the USB storage medium in the interface.
3. Import the labware (see Importing labware on p. 93).
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9.5
Special labware features in epBlue
9.5.1
Location B0 on the worktable
The dispensing tool cannot reach the location B0 on the worktable completely. The dispensing tool can only
fill the right locations of plates and ReservoirRacks.
 Use the location B0 for reservoirs.
 Use the location B0 to stack plates.
9.5.2
Tubes
Labware types in epBlue: Racks with Tubes > Tubes.
Reaction vessels are not available in epBlue as independent labware.
9.5.3
MTP plates
Labware types in epBlue: Plates > MTP.
Measuring the filling level is not available for MTP plates with 384 wells.
For MTP plates with 96 wells, measuring the filling level takes a long time.
9.5.4
PCR plates
Labware types in epBlue: Plates > PCR.
Labware
Labware types
PCR plates with 96 wells
PCR 96
PCR plates with 384 wells
PCR 384
9.5.5
epT.I.P.S. Motion
Labware types in epBlue: Tips.
Pipette tips with filters have a "f" added to their name in epBlue.
Pipette tips
Example of names in epBlue
without filter
tip1000
with filter
tip1000f
Racks and Safe Racks have the same labware definition.
epBlue Labware Editor - Creating and managing labware
epBlue™ with EasyCon
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9.5.6
Racks
Labware types in epBlue: Racks with Tubes
Equip the racks with vessels before you use them in an application.
9.5.7
Rack 96
Labware types in epBlue: Racks with Tubes > Racks > Rack96_1_5 - 2_0
The labware definition of the Rack 96 only applies to the Rack. epBlue does not have a labware definition
for the Rack 96 and inserted reaction vessels.
 Create a labware definition from the labware definition of the Rack 96 and the labware definitions of the
used reaction vessels.
 Position the Rack 96 in row B and C, e.g. on locations B2 and C2.
epBlue detects that the Rack 96 occupies two locations on the worktable.
 Define the properties of the Rack 96 in the same way as the properties of other labware. E.g. you can
define the setting of the optical sensor.
9.5.8
Rack LC
Labware types in epBlue: Plates > Tube Plates 96
9.5.9
ReservoirRack
Labware types in epBlue: ReservoirRacks with Modules
Only irregular patterns may be set if the ReservoirRack is equipped with different ReservoirRack modules
and reservoirs.
Regular patterns may be set if the ReservoirRack is equipped with identical ReservoirRack modules or
identical reservoirs.
For each reservoir and each ReservoirRack module, the Detect volumes function of the optical sensor can be
activated.
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9.5.10
ReservoirRack Module TC
First, equip a ReservoirRack module with vessels. Then place the equipped ReservoirRack module in the
ReservoirRack.
The software only positions equipped ReservoirRack modules in a ReservoirRack.
Labware types in epBlue for unequipped ReservoirRack modules can be found in the folder: Racks with
Tubes > ThermoRacks + Modules.
Labware types in epBlue for reservoirs and equipped ReservoirRack modules can be found in the folder:
ReservoirRacks with Modules > Modules with Tubes.
Module
Labware type
ReservoirRack Module TC Ø 29 mm
Module TC 29mm
ReservoirRack Module TC Ø 17 mm
Module TC 17mm
ReservoirRack Module TC Reservoir 100 mL
Module TC Reserv100ml
ReservoirRack Module TC Reservoir 30 mL
Module TC Reserv30ml
ReservoirRack Module TC Ø 16 mm
Module TC 16mm
ReservoirRack Module TC Ø 12 mm
Module TC 12mm
ReservoirRack Module TC Safe Lock
Module TC Safe Lock
ReservoirRack Module TC PCR 0.5 mL
Module TC PCR 0_5ml
ReservoirRack Module TC PCR 0.2 mL
Module TC PCR 0_2ml
ReservoirRack module TC Eppendorf Tubes 5 mL
TC 5 ml module
9.5.11
Thermoracks
Labware types in epBlue: Racks with Tubes
The thermoracks must be equipped with vessels before they can be used in an application.
Thermoblock/thermorack PCR 96: This labware is defined as a thermoblock and a thermorack in the
labware library.
epBlue Labware Editor - Creating and managing labware
epBlue™ with EasyCon
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9.5.12
Thermorack Rotor/Tubes
Order no.:
• Qiagen rotor disc 72: Order no.: 981301
• Qiagen rotor disc 100: Order no.: 981311
The tubes in the Qiagen rotor disc are very narrow.
With narrow tubes, the pipette tips can get jammed in the bottom section of the tubes. For this reason, the
Qiagen rotor disc has a higher bottom tolerance. Detailed information on the bottom tolerance can be found
in the labware definition.
 Only use the single-channel dispensing tool TS 50 when working with the Qiagen rotor disc.
You can only pipette into the narrow tubes of the Qiagen rotor disc with a single-channel dispensing tool
TS 50.
9.5.13
TipHolder
Labware types in epBlue: Adapter > Height.
The TipHolder is an adapter that holds the epT.I.P.S. Motion Reloads.
epT.I.P.S. Motion Reloads 1000 μL are positioned on the worktable using the TipHolder. To use epT.I.P.S.
Motion Reloads 50 μL and epT.I.P.S. Motion Reloads 300 μL with the TipHolder, program a height adapter
40 mm on the epBlue worktable. Pull the epT.I.P.S. Motion Reloads 50 μL or 300 μL onto the height
adapter.
9.5.14
Thermoadapter
Labware types in epBlue: Adapter > Thermo
In the software you can find the Thermoadapter LC Sample+Cartridge under Thermoblocks with plates.
The thermoadapter and plate combination is created during the editing of the worktable or via a transfer
command.
With the "Thermoadapter with skirted PCR plate" combination, the plate can be transported by the gripper.
The thermoadapter cannot be transported by the gripper.
For the Thermoadapter Frosty, select a skirted PCR plate for the location. The cooling aggregate is not
named in epBlue. In the software, the Thermoadapter Frosty can be found under Adapter > Height.
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9.5.15
Universal Disc by Focus Diagnostics
Order no.:
• Universal Disc: Order number at Focus Diagnostics: MOL1401
The epMotion fills the positions on the Universal Disc with sample material. The epMotion cannot extract
sample material from the positions on the Universal Disc.
 Work in the Pipette dispensing mode to add a second substance.
If you use the Multidispense dispensing mode, the liquid transfer is not performed in an optimum way.
9.5.16
Thermoblocks
Thermoblocks are heat-conductive adapters that can be transported with the gripper.
There are two types of thermoblocks available.
• Thermoblocks with plates are thermoblocks that are permanently connected with the plate.
• Thermoblocks are thermoblocks, into which the plates can be inserted.
Transport with the gripper
Thermoblock without plates
Thermoblock with plates
Thermoblock with plate
x
x
Thermoblock without plate
x
-
Insert plate in the thermoblock
x
-
Remove plate from the
thermoblock
x
-
9.5.17
Thermoblock PCR 96 OC
If you use the Thermoblock PCR 96 OC with a twin.tec PCR plate 96, semiskirted, you can use the TMX
template PCR 96 NGS. With the TMX template you can mix with a mixing speed of 1600 rpm for 2 minutes.
At a mixing speed of 1600 rpm, the maximum working volume is 140 μL. If the working volume is higher,
the liquid may be spilled.
The maximum mixing speed is 1700 rpm.
epBlue settings - Checking components and making the basic settings
epBlue™ with EasyCon
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10
epBlue settings - Checking components and making the basic settings
This chapter describes how to check components of the epMotion and make the basic settings.
Prerequisites
• epMotion is ready for operation.
• epBlue is started.
• The start screen will be displayed.
1. Press on the Settings symbol on the start screen.
2. Select the function.
10.1
Tool interlock
The Tool interlock function can be used to check the locking mechanism of the dispensing tools and
gripper.
Abb. 10-1: Tool interlock function
Fig. 10-1: Tool interlock function
1 Zero value sensor field
0 = Dispensing tool is not in home position. 1 =
Dispensing tool is in home position.
5 Refresh button
Update fields 1 – 4
6 Lock button
Lock the dispensing tool
2 Tool detection sensor field
0 = Dispensing tool not available. 1 = Dispensing
7 Unlock button
tool available.
Unlock the dispensing tool
3 Interlock field
OPEN = Locking mechanism opened.
CLOSED = Locking mechanism closed.
4 Current Tool field
Name of the tool used
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10.1.1
Lock the tool
1. Slide the tool into the tool holder from below.
2. Hold the tool tightly At the same time, press the Lock button.
The tool holder locks the tool.
10.1.2
Unlocking the tool
1. Hold the tool tightly At the same time, press the Unlock button.
The tool holder unlocks the tool.
2. Remove the tool.
10.2
Dosing device
The Dosing device function can be used to read out the number of stroke movements of a dispensing tool.
Abb. 10-2: Dispensing Device function
Fig. 10-2: Dispensing Device function
Tool used
Name of the tool
Tool path
Path of the labware definition of the tool
10.2.1
Strokes
Number of stroke movements of the dispensing
tool
Reading out the tool
Prerequisites
A tool is in the tool holder.
 To read out the tool data, click on the Read button.
epBlue settings - Checking components and making the basic settings
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10.3
Gripper
The Gripper function can be used to open and close the gripper. Labware can be removed from the gripper
if necessary.
Abb. 10-3: Gripper function
Fig. 10-3: Gripper function
Distance field
Distance of gripper arms
Set button
Confirm new distance of gripper arms
Gripper distance (µm) field
Enter new distance of gripper arms
Export field
Exports data
10.3.1
Moving the gripper arms
Prerequisites
• The gripper is located in the tool holder.
1. To move the gripper arms, enter a value in the Gripper distance field. The value must be approx. 20%
larger than the value in the Distance field.
2. Confirm your entry with the Set button.
The gripper can now be moved.
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10.4
UV lamp
The UV lamp is used to decontaminate the epMotion worktable. An irradiation cycle lasts 15 min.
10.4.1
Decontaminating the worktable
CAUTION! Risk to health due to UV radiation.
UV radiation causes eye and skin damage.
UV radiation may escape outward through the ventilation slits in the rear panel of the device.
 Wear your personal protective equipment when you are near the ventilation slits.
NOTICE! Damage to plastic due to UV radiation.
Plastics become brittle as a result of UV radiation exposure.
 Remove the dispensing tools, labware, and waste container from the worktable before
switching on the UV lamp.
Prerequisites
•
•
•
•
•
The epMotion is equipped with a CleanCap.
The dispensing tools, labware and waste container have been removed from the worktable.
The front hood is closed.
The epMotion is ready for operation.
epBlue has been started.
1. Select the Settings symbol on the start screen.
2. Select the UV lamp function.
3. Click on the UV Light OFF button.
The UV Lamp window appears.
4. Check to see if the worktable is empty and the front hood is closed.
5. Click on the OK button to start the disinfection.
epBlue settings - Checking components and making the basic settings
epBlue™ with EasyCon
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Abb. 10-5: UV Light button
Abb. 10-4: Lamp symbol in status area
Fig. 10-4: Lamp symbol in status area
Fig. 10-5: UV Light button
The tool holder moves. The UV lamp will be switched on.
The UV Light button shows the remaining irradiation period. The lamp symbol appears in the status area.
10.5
HEPA air filter
Air is drawn in using a fan. The air drawn in is filtered using the HEPA air filter. A continuous and filtered
air flow is guided into the epMotion workspace. The air flow prevents the workspace from being
contaminated with outside air.
10.5.1
Switching on the HEPA air filter
WARNING! Risk of infection due to aerosols.
If you work with the HEPA filter, a continuous air flow is blown from the device workspace to
the environment. The air flow can transport aerosols and contaminate the ambient air.
 Do not use the HEPA filter when working with biohazardous substances.
 Disinfect contaminated surfaces in the workspace before switching on the HEPA filter.
Prerequisites
•
•
•
•
•
The epMotion is equipped with a CleanCap.
All surfaces in the workspace are disinfected.
The front hood is closed.
The epMotion is ready for operation.
epBlue has been started.
 Select the Settings symbol on the start screen.
 Select the HEPA filter function.
 Press the HEPA filter ON button.
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Abb. 10-6: Filter symbol in the status area
Fig. 10-6: Filter symbol in the status area
The HEPA air filter is switched on. The filter symbol appears in the status area.
The HEPA air filter remains switched on if you exit the HEPA filter function. Now start your application.
10.5.2
Switching off the HEPA air filter
Prerequisites
• The HEPA air filter is running.
• The HEPA filter function on the epMotion serial number > Settings tab was selected.
1. Press the HEPA filter ON button.
The HEPA air filter is switched off.
2. Alternatively, you can switch off the HEPA air filter when you switch off the epMotion using the mains/
power switch.
epBlue Administration
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11
epBlue Administration
As an administrator, you can save the database, create print templates and make basic settings for the
optical sensor.
11.1
epBlue Administration
11.1.1
Administration Starting epBlue
Prerequisites
• The epBlue start screen will be shown.
1. Click on the Administration symbol.
The User Login window appears.
2. Select the administrator account.
3. Enter the Admin123! password in the Password field.
4. Press the Login button.
epBlue Administration is started. The Administrator area appears.
11.1.2
Exit epBlue Administration
1. In the File menu, select the Exit to Start Screen entry.
The Administration tool will be ended. The start screen appears.
11.2
Changing the device name
In the Devices window you will find information on the status and the configuration of the epMotion. The
Devices > Settings > Detail information > Nickname shows the name of the epMotion. The serial number will
be assigned as the name by default. You can change the name of the device.
You can check the settings if you are logged in as an administrator.
1. Press on the Administration symbol on the start screen.
2. On the Devices tab, select the Settings function.
The Detail Information window will appear.
3. Change the device name in the Nickname field.
4. To save the changes, press the Save button.
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11.3
Print templates
Print templates can define how your print-outs and PDF documents look.
Abb. 11-1: Print Header window
Fig. 11-1: Print Header window
Current preview setting drop-down list
Select a print template.
Show preview button
Show a preview.
Add button
Create a print template.
... button
Import a graphic for the header.
Edit button
Change a print template.
Save button
Save a print template.
Remove button
Delete a print template.
Cancel button
Cancel the process.
Save as default button
Save print template as default.
epBlue Administration
epBlue™ with EasyCon
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11.3.1
Creating a print template
Prerequisites
• You are logged in as Administrator or Admin Lab.
• epBlue is started.
1. Click on the Administration symbol on the start screen.
2. Select the Print Header function.
The Print Header window appears.
3. Click on the Edit print header template button.
4. Enter your company's address in the Company Information field.
5. Import a graphic for the header. by clicking on the
button.
epBlue only imports graphics in the formats GIF, JPEG, and PNG.
6. To show a preview, click on the Show preview button.
7. To save the template, click on the Save button.
8. Enter the name of the template and confirm it using the OK button.
The template will be saved.
11.3.2
Saving print templates as default
Prerequisites
• You are logged in as Administrator or Admin Lab.
• epBlue is started.
1. Click on the Administration symbol on the start screen.
2. Select the Print Header function.
The Print Header window appears.
3. Select the print template in the Current preview settings menu.
4. Click on the Set as default header button.
The print template is saved as standard.
11.3.3
Deleting a print template
Prerequisites
• You are logged in as Administrator or Admin Lab.
• epBlue is started.
1. Click on the Administration symbol on the start screen.
2. Select the Print Header function.
The Print Header window appears.
3. Select the print template in the Current preview settings menu.
4. Press the Remove button.
The print template is deleted.
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11.4
Data backup
epBlue saves applications, protocols, the labware library and information on user accounts in a database.
The Backup function can be used to create a backup copy of the database. The backup copy can be used to
restore the database after a system failure.
You can save data and load a backup copy if you are logged in as administrator.
NOTICE! Data loss due to missing data backup or incorrect storage of data carriers.
User accounts, applications, labware and logs are stored in one database. If this database gets
damaged (e.g. due to a hardware defect), the information is lost.
Eppendorf is not liable for data loss of data. Eppendorf is not liable for damage arising as a
result of data loss.
 Backup the database regularly. Only use the epBlue software backup.
 Save stored data on external data carriers.
 Store the storage media according to the manufacturer's specifications.
11.4.1
Backing up the database
Proceed as follows:
Prerequisites
• A USB storage medium is connected to the EasyCon Tablet.
• epBlue is started.
• You are logged in as administrator.
1. Press the Settings symbol on the start screen.
2. Select the Backup and Restore tab.
3. Select Backup.
4. Press the
button.
5. Use that name for the backup or select a new name.
6. Start the backup using the Backup button.
epBlue Administration
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11.4.2
Restoring the database
You can import your backup copy in epBlue. The database will be restored to the status at the time of the
backup.
All data in epBlue is overwritten when you import the backup copy. Changes made after this
backup copy was created will be lost.
Abb. 11-2: Restore window
Fig. 11-2: Restore window
Source Full Backup
Location where a backup copy is saved.
Status field
Shows if the software is ready or damaged.
Details field
Start button
Imports the backup copy in epBlue.
Progress bar
Progress of backup
Prerequisites
• epBlue is started.
• You are logged in as administrator.
1. Connect the USB storage medium to the control device.
2. Press the Settings symbol on the start screen.
3. Select the Backup and Restore tab.
4. Select Restore.
5. Press the
button.
6. Select a backup copy with the *.bak format.
7. To import the backup copy into epBlue, click on the Restore button.
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11.4.3
Importing a database from epBlue version 40.X
You can use this function to import databases from the epBlue versions 40.X into epBlue from version 40.4.
Prerequisites
• epBlue is started.
• You are logged in as administrator.
1. Press the Settings symbol on the start screen.
2. Select the Backup and Restore tab.
3. Select Migrate 40.x database.
4. Press the
button.
5. Select a backup copy with the bak format.
6. To import the database into epBlue, press the Migrate button.
11.5
Temperature control unit X
The Temperature control unit X function enables you to check the thermal modules of the epMotion.
Abb. 11-3: Temperature control unit function
Fig. 11-3: Temperature control unit function
Desired Temperature
Shows the set temperature of the thermal
module.
Remain time
Shows the time remaining until the end of
temperature control (in min).
Actual Temperature
Shows the set temperature of the thermal
module.
Temp reached after
Shows the time required to reach the set
temperature.
Actual current
Shows the current strength in the thermal
module.
New Temperature
Set temperature (–5°C to 110°C)
Time
Shows the temperature control duration in min.
New time
Temperature control duration (0 to 99 min)
epBlue Administration
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The current status is shown in the State area.
You can make settings in the Set Temperature area.
1. Enter the set temperature in the New Temperature field.
2. Enter the test duration in the New time field.
3. Click on the Start button. The thermal module heats or cools until the set temperature has been reached
or the set time has elapsed. If the set temperature is reached before the set time has elapsed, the
thermal module maintains the set temperature until the end of the test.
4. To update the display during the test, press the Refresh button.
5. To activate the fan, press the Fan On button.
11.6
Levelsensor options
The Levelsensor options function is used to define the functions the optical sensor will execute. This setting
is used as the default.
The time for the application is reduced when the optical sensor is switched off. The volume must be entered
when the application is started.
Abb. 11-4: Levelsensor options function
Fig. 11-4: Levelsensor options function
Detect volumes radio
button
The optical sensor measures the filling volume. It is determined in the properties
of the labware, in which positions the optical sensor measures the filling volume.
Use required minimum
volumes radio button
epBlue assumes that all vessels are filled with the minimum required volume for
this run.
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Input volumes manually Enter filling volume manually.
radio button
Slider Detect tips
The optical sensor checks, in which positions of the labware pipette tips are
available.
Check labware
placement slider
The optical sensor checks if labware is positioned on the worktable.
Information on the optical sensor (see Optical sensor on p. 77).
11.7
ThermoMixer
This function enables you to check the Eppendorf ThermoMixer module.
NOTICE! Contamination of samples and the device due to excessive speeds.
If the speed is set too high, liquid sprays out of the vessels. Labware may become loose from
the holder and fly around.
 Observe the labware maximum speeds.
Tab. 11-1: Maximum speed for labware on the Eppendorf ThermoMixer module
Labware
Maximum speed in rpm
Vessels
1000
Deepwell plates with 96 wells
1200
Deepwell plates with 384 wells
1200
Microplate with 6 wells
1000
Microplates with 96 wells
2000
Microplates with 384 wells
2000
PCR plate with 96 wells
2000
PCR plate with 384 wells
2000
ReservoirRack
Mixing not possible
Thermorack or Thermoblock
1000
Thermoblock 96 with a semi-skirted PCR plate for 250 μL
1700
Thermoadapter for Microplate 96/U/V with Eppendorf Microplate 96/V
1600
Thermoadapter for Microplate 96/U/V with Eppendorf Microplate 96/U
1600
Thermoblock PCR 96 OC
1700
Thermoadapter for 96 wells
1000
Thermoadapter for 384 wells
1000
Thermorack TMX
1300
PrepRack
1300
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11.7.1
Checking the mixing function of the Eppendorf ThermoMixer module
Abb. 11-5: Mixer Control Thermomixer function
Fig. 11-5: Mixer Control Thermomixer function
RPM field
Start button
Speed of the Eppendorf ThermoMixer modules in
Stop button
rpm.
Time field
Duration of the mixing process.
Remaining time field
Remaining time in the mixing process.
1. Define the speed and temperature in the Parameter area.
2. To check the mixing function, press the Start button.
The Eppendorf ThermoMixer module mixes until the time has elapsed or until the test has been exited
using the Stop button.
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11.7.2
Checking the temperature control of the Eppendorf ThermoMixer module
Abb. 11-6: Thermomixer temperature control function
Fig. 11-6: Thermomixer temperature control function
Desired Temperature field
Temp reached after field
Set temperature of the Eppendorf ThermoMixer
Time until the set temperature is reached.
module.
New Temperature field
Actual Temperature field
Enter a new set temperature. 4 °C– 95 °C range.
Actual temperature of the Eppendorf
New time field
ThermoMixer module.
Enter a new mixing time. 0 s - 99 min range.
Actual current field
Start button
Current strength via the thermal module.
Start the test.
Time field
Fan on button
Mixing time in min.
Switch on the fan.
Remain time field
Time until the end of the mixing process (in min).
The current status is shown in the State area.
You can make settings in the Set Temperature area.
1. Enter the set temperature in the New Temperature field.
2. Enter the mixing time in the New time field.
3. Press the Start button.
The Eppendorf ThermoMixer module tempers the labware to the set temperature. If the temperature is
reached before the mixing time has elapsed, the Eppendorf ThermoMixer module maintains a constant
temperature until the end of the test.
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11.8
Magnetic separation
The Magnetic Separation function can be used to check the magnetic finger module.
Abb. 11-7: Magnetic Separation function
Fig. 11-7: Magnetic Separation function
Home
Move the magnetic finger module to the home
position.
Draw Out
Draw out the magnetic fingers.
Draw In
Draw in the magnetic fingers.
125
126
epBlue Administration
epBlue™ with EasyCon
English (EN)
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
12
epBlue - Software error messages
This chapter contains an overview of the software error messages, causes and solutions.
12.1
Error messages
If an error occurs, first try the following:
1. Switch off epMotion.
2. Restart the epMotion.
3. Execute the application again.
If the error occurs again, contact Eppendorf Service or your dealer. The addresses can be found on our
webpage www.eppendorf.com.
Code
Problem
Cause
Solution
Message
without code
Make sure that there is
nothing in the gripper: If
any labware is held by the
gripper arms remove this
item manually; then press
Ok.
Method run was aborted;
If the gripper is still holding
gripper is still fixed in the tool any items:
holder; before tool holder
1. Remove the item manually
moves further (e.g., to its
by opening the two gripper
home position) the message
arms manually and
appears.
removing the labware
manually.
2. Press the Ok button.
The gripper expects that the
labware has been removed and
now moves to the home
position.
 To remove the dispensing
(without code) Because all tool holder
Method run was aborted;
tool, follow the instructions.
positions are occupied, the Dispensing tool is still fixed in
tool has to be removed
the tool holder.
manually:
Press Ok, open the hood
and be prepared to grasp
the tool manually as the
tool holder opens and
drops the tool.
0x0600,
0x060E
Tool did not find home
0x0C01
Volume too large for this
tool
• Home position for the tool
is not found.
• No tool inserted.
• Tool damaged.




Insert tool.
Check tool.
Reboot and try again.
If error occurs again: Call
local Eppendorf Service.
Volume to be dispensed is too  Call local Eppendorf
large for the selected tool.
Application Support.
Possible causes:
• Errors in tool files.
• Errors in liquid type files.
127
128
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
Cause
Solution
0x0C02
Volume too small for the
dispensing tool.
Volume to be dispensed is too  Call local Eppendorf
small for the selected tool.
Application.
Possible causes:
• Errors in tool files.
• Errors in liquid type files.
0x0C08
Tool dimension unknown
Tool dimension values
unknown. Labware outdated
or corrupt.
 Make sure that all labware
is of the latest version.
0x120A
Program aborted by user
User pressed the Abort button  Error message was
during program run.
information for the user
that he aborted the
program.
0x1221
The hood was opened
while the program was
stopped
See error message.
 Close hood.
0x1222
Transfer allowance was
prematurely deactivated
during program
initialization
See error message.
 Start program again.
0x128B
Carrier: steps lost in x
• Carrier collision.
• Carrier mechanics out of
order.
 Ensure that the worktable
equipment in epBlue is
identical to the equipment
in epMotion, then restart
the application.
 If the error persists, call
Eppendorf Service.
0x128C
Carrier: final position in y
not found
• Problems in carrier
 Call Eppendorf Service.
movement in y-axis
(sluggish movement or no
movement at all).
• Light barrier for carrier in
y-axis defective.
0x128E
Carrier: steps lost in y
• Carrier collision.
• Carrier mechanics out of
order.
 Ensure that the worktable
equipment in epBlue is
identical to the equipment
in epMotion, then restart
the application.
 If the error persists, call
Eppendorf Service.
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
0x1294
Carrier: steps lost in z
0x1295
Carrier:
Steps lost in z before
picking up tip.
Cause
Solution
• Carrier collision.
• Carrier mechanics out of
order.
 Ensure that the worktable
• Tip was still on pipette tool
when tool started to pick
up a new tip.
• Tip rack not placed
correctly on the worktable.
• Mechanical problems of
carrier.
 Remove tips from tools.
 Place tip rack correctly, and
equipment in epBlue is
identical to the equipment
in epMotion, then restart
the application.
 If the error persists, call
Eppendorf Service.
plane, on the worktable.
In other cases:
 Call local Eppendorf
Service.
0x1296
Maximum number of tool
cycles exceeded
See error message.
 Have the tool serviced.
0x1297
Danger of collision
When running the
programmed application, the
tool carrier system touches
racks or other labware on the
worktable; e.g., during
pipetting, the optical sensor
may touch a long tube on the
adjacent position; possible
reasons:
• A low plate (microplate) is
located next to a high
Tube Rack.
• The 50 μL or 300 μL tip is
programmed to move
almost to the bottom of a
very long tube with
another long tube in the
adjacent position.
 Program the labware on the
Tool not calibrated
Have the tool serviced.
worktable so that high and
low labware is not adjacent.
 Program the labware so
that the 30 μL or 300 μL tip
does not have to move
deeply into a long vessel.
 If possible: use higher
volumes in the long vessels.
 If possible: use longer tips
for the long vessels.
0x1298
Tool not calibrated
0x1299
Invalid number of samples Value for number of samples  Insert an admissible value
not permissible.
for number of samples.
0x129A
Tip too small
Reagent Transfer: Used tip is
too small.
0x129B
Source vessel too small
Reagent Transfer: Used source  Use a larger vessel.
vessel is too small.
 Use a larger tip.
129
130
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
Cause
Solution
0x12B4
Temperature unit in
location C1 not active
In the wait command, the wait  Insert temperature
for temperature parameter
command before wait
was edited for location C1
command or delete the wait
even though no temperature
for temperature parameter
command for C1 had been
for this location.
edited before.
0x12B5
Temperature unit in
location C2 not active
In the wait command, the wait  Insert temperature
command before wait
for temperature parameter
command or delete the wait
was edited for location C2
for temperature parameter
even though no temperature
for this location.
command for C2 had been
edited before.
0x12B6
Temperature unit in
location C3 not active
In the wait command, the wait  Insert temperature
command before wait
for temperature parameter
command or delete the wait
was edited for location C3
for temperature parameter
even though no temperature
for this location.
command for C3 had been
edited before.
0x12B7
Temperature unit in
location C1 too hot for
labware (temperature
command in this method)
The temperature of location  Select a labware item that is
more resistant to high
C1 (according to a
temperatures (e.g., made
temperature command in this
from polypropylene or
application) is too high for the
polycarbonate).
labware placed on this
location.
0x12B8
Temperature unit in
location C2 too hot for
labware (temperature
command in this method)
The temperature of location  Select a labware item that is
more resistant to high
C2 (according to a
temperatures (e.g., made
temperature command in this
from polypropylene or
application) is too high for the
polycarbonate).
labware placed on this
location.
0x12B9
Temperature unit in
location C3 too hot for
labware (temperature
command in this method)
The temperature of location  Select a labware item that is
more resistant to high
C3 (according to a
temperatures (e.g., made
temperature command in this
from polypropylene or
application) is too high for the
polycarbonate).
labware placed on this
location.
0x12BA
Temperature unit in
location C1 too hot for
labware (temperature
command in previous
method)
The temperature of location  Select a labware item that is
more resistant to high
C1 (according to a
temperatures (e.g., made
temperature command in a
from polypropylene or
previous application with
polycarbonate).
parameter keep temperature
after method run set active) is  Change previous
application to not maintain
too high for the labware
temperature after
placed on this location.
application run.
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
Cause
Solution
0x12BB
Temperature unit in
location C2 too hot for
labware (temperature
command in previous
method)
The temperature of location  Select a labware item that is
more resistant to high
C2 (according to a
temperatures (e.g., made
temperature command in a
from polypropylene or
previous application with
polycarbonate).
parameter keep temperature
after method run set active) is  Change previous
application to not maintain
too high for the labware
temperature after
placed on this location.
application run.
0x12BC
Temperature unit in
location C3 too hot for
labware (temperature
command in previous
method)
The temperature of location  Select a labware item that is
C3 (according to a
more resistant to high
temperature command in a
temperatures (e.g., made
previous application with
from polypropylene or
parameter keep temperature
polycarbonate).
after method run set active) is  Change previous
application to not maintain
too high for the labware
temperature after
placed on this location.
application run.
0x12D0
Parameter conflict:
Elution volume too large
for this tool
Sample transfer with elution  Select a tip large enough
from filter option:
for picking up the liquid as
Volume to be aspirated is too
well as the additional
large for the tip used.
volume of air to be
aspirated when using this
option.
0x12D1
Parameter conflict:
Elution volume too large
for destination vessel or
well
Option elution from filter:
volume is too large for the
vessel used.
 Select a tool large enough
when using this option.
0x12E1
Parameter conflict:
See error message.
Pre-wetting not possible
when aspirate from bottom
is selected
 Change application.
0x12E2
Parameter conflict:
See error message.
Pre-wetting not possible
when dispense from top is
selected
 Change application.
0x12E3
Parameter conflict:
Pre-wetting not possible
when elution from filter is
selected
A liquid type using a
 Change application.
pre-wetting step (e.g., ethanol
98%) cannot be used in
combination with the elution
from filter parameter in a
sample transfer command.
0x12E4
Transport of labware:
danger of collision
 Do not stack more than 5
Danger of collision when
racks in a location.
transporting a stack of several
racks. The upper rack would
collide with the carrier.
131
132
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
Cause
Solution
0x12E6
Level too high
The liquid level would be
higher than the vessel after
dispensing.
 Adjust the liquid to be
 See error message.
dispensed to the vessel.
0x12E7
Opening the hood is not
allowed when putting
down tool.
Switch off power, then
switch on again to restart
method.
See error message.
0x12E9
Tool not locked
This can only happen with the  Close tool lock.
5070. The tool lock is not
properly closed.
0x12F3
The Eppendorf
ThermoMixer module is
too hot for the labware.
Temperature command in
this method.
The thermal module of the
Eppendorf ThermoMixer
module is is too hot for the
selected labware.
Choose a lower temperature.
0x12F4
The Eppendorf
ThermoMixer module is
too hot for labware.
Temperature command in
previous method.
The thermal module of the
Eppendorf ThermoMixer
module is is too hot for the
selected labware.
Choose a lower temperature.
0x12F7
Waiting for Eppendorf
ThermoMixer module
The application is waiting for Wait until Eppendorf
the Eppendorf ThermoMixer ThermoMixer module function
module.
has finished.
0x12F8
The selected mixing speed The mixing speed is not
is not possible with this
allowed for the selected
labware
labware.
Select another labware or
mixing speed.
0x1500
Too big vessel index in
location:
A tube is to be accessed for
which the index is greater
than the number of tubes on
the plate/rack/holder.
 Error during creation of the
<rack name> is not
accessible for tools in
location ...
Rack is a lower part of a
 Change application so that
labware stack. Therefore, the
the rack is accessible.
tool has no access.
0x1504
application.
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
Cause
Solution
0x1509
 Supply less volume in the
Liquid volume too large for Total volume supplied in a
vessel.
vessel in location:...
source vessel is larger than
needed or larger than vessel.  Change application.
 When verifying the total
volume needed for the
source or destination, take
into account the additional
aspirated volume when
using multidispense mode.
epMotion 5070 only:
 Set Detect volumes to off for
racks that are in park
positions at the beginning
of the procedure.
0x150A
Liquid volume too low for
vessel in location: ...
Total volume supplied by the  Calculate the total volume
user in a source vessel is
for the source or
smaller than the volume
destination vessel and
needed for a sample transfer,
select a suitable vessel. For
reagent transfer or mix
additional aspirated volume
command (total volume =
with multidispense mode,
volume to be aspirated +
refer to manual.
 Consider that the software
remaining volume for this
may calculate higher
vessel + (in case of
remaining volumes in some
multidispense mode:)
cases to avoid crashes.
additional aspirated volume.
 Set Detect volumes to off for
racks that are in park
positions at the beginning
of the procedure.
0x150B
Optical sensor:
Liquid volume too low in
location: ...
See above (error 0x150A).
0x150D
Optical sensor: Plate could
not be found in location: ...
Optical sensor: Rack could
not be found in location: ...
The rack programmed for this  Place the rack onto the
location could not be found by
locations as edited in the
the optical sensor; possible
corresponding application,
causes:
or:
 Make sure that the rack is
• Rack not placed onto
placed plane on the
location (wrong rack code
worktable surface, or:
or wrong rack height).
• Rack in wrong orientation.  Rotate rack 180° (front to
back) and place it back onto
• Problems related to the
the worktable location, or:
optical sensor function.
 Call local Eppendorf
Service.
 See above (error 0x150A).
133
134
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
Cause
Solution
0x150E
Optical sensor:
The tip rack programmed for  Place the tip rack onto the
Tips could not be found in this location could not be
locations as edited in the
location ...
found by the optical sensor;
corresponding application,
possible causes:
or:
• Tip rack not placed onto  Call local Eppendorf
Service.
location.
• Problems related to the
optical sensor function.
0x1510
Optical sensor: Nothing
See error message.
could be found in location:
 Place the labware
programmed for this
location on the worktable.
If error occurs again:
 Call local Eppendorf
Service.
0x1512
Tip type ... is not placed on Tips that are needed
the worktable
according to the application
are not available on the
worktable.
 Place the tip tray
programmed for this
location on the worktable.
If error occurs again:
 Call local Eppendorf
Service.
0x1513
Position is out of range
The position to be addressed  Change application.
by the tool carrier is outside If error occurs again:
of its available range.
 Call local Eppendorf
Possible cause: Rack in park
Service.
position is programmed to be
addressed by the dispensing
tool.
0x1514
Optical sensor: Rack in
wrong orientation in
location ...
The tub holder has been
 Rotate tub holder 180° and
placed on the worktable in the
place it back onto the
wrong direction.
worktable; restart the
application.
0x1515
Tool cannot be used for
rack in location ...
Distance between tip cones of  Change application.
the liquid handling tool does
not match the distance
between vessels (e.g., 24
tubes - rack does not fit the
eight-channel dispensing
tool).
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
Cause
Solution
0x1516
No vessel in location: ...
Vessels that are needed
according to the application
are not available on the
worktable (vessel/rack
combination).
 Place the vessel/rack
combination programmed
for this location on the
worktable.
If error occurs again:
 Call local Eppendorf
Service.
The item cannot be picked up  Change application.
by the gripper.
0x1518
Rack is not tangible in
location: ...
0x1519
Tip is too thick for vessel in Diameter of the destination
location: ...
vessel is too small for the tip
when dispensing the liquid.
 Select other tips or vessels
in the application.
 Select dispense from top in
the options of the liquid
handling command.
0x151A
Optical sensor:
The optical sensor has
 Remove the cap from the
There is a cap on vessel in detected a cap on a vessel
vessel and start the run
location: ...
when trying to detect a liquid
again.
level.
0x151B
Optical sensor: There is an Relates to vessels that are
 Place the vessel onto the
incorrect vessel in location: equipped with a readable
location as edited in the
...
code (e.g., Eppendorf tubs):
corresponding application,
The rack programmed for this
or:
location could not be found by  Call local Eppendorf
Application Support.
the optical sensor; possible
causes:
• Wrong vessel.
• Problems related to the
optical sensor function.
0x151C
Optical sensor:
Vessel too high for level
detection in location: ...
Level detection for very high
vessels is not possible.
 Switch off the level
detection for this vessel.
 Use level detection only for
vessel/rack equipment with
a total height below
103 mm.
0x151E
Detected volume is out of
detection range ...
Normally a system/hardware
error (malfunction of the
optical sensor); but may also
be caused by filling a vessel
up to the total vessel height.
 Do not fill vessels above the
specified maximum filling
volume.
In other cases:
 Call local Eppendorf
Service.
0x151F
Labware stack too high in
location:
Maximum pieces which
may be piled:
A maximum of 5 racks can be  Do not stack more than 5
stacked in a location. Placing
racks in a location.
more than 5 racks in a
location.
135
136
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
Cause
0x1522
Transport of the selected
labware to this position or
stack is not allowed
Position xxx
Different reasons to forbid
 Change application.
this transport; main reason:
transport of the selected
labware to this position or
stack might result in
instability of the stack, or the
resulting stack might become
too high.
0x1523
Labware stack too high in Total height of labware stacks  Edit application to avoid too
high labware stacks.
location: xxx
(like plates on adapters) is too
Maximum height: xxx mm high.
0x1526
No open position available When trying to deposit the
 Clear at least one position
for depositing tool
dispensing tool after use the
on the worktable to accept a
tool holder did not find a free
dispensing tool.
position for the tool.
0x1529
Dispensing tool could not
be found.
Dispensing tool required
for this method:...
0x152A
See error message.
Solution
 Provide the dispensing tool
that is needed on the
worktable at its proper
position.
Gripper could not be found See error message.
 Provide the gripper on the
worktable at its proper
position.
0x152D
Tip too short
Tip does not reach the liquid  Select other tips or vessels
Select other tips or vessels level at the beginning or
in the application.
in the method.
during the course of the liquid
handling command.
0x152F
Labware stack too high in
location: xxx
Maximum pieces which
may be piled: 5
A maximum of 5 racks can be  Change application.
piled in a location. After a
transport command, the
labware stack would be too
high.
0x1530
Dispensing in pile not
allowed in location: xxx
See error message.
 Change application.
0x1531
Transport of this pile not
allowed in location: xxx
Do not transport a stack.
 Change application.
0x1581
Optical sensor:
Liquid level could not be
detected in location: ...
Error in level detection.
 Repeat measurement.
0x1602
Program too long
Maximum size of steps is
reached.
 Delete commands that are
not necessary before insert
new commands.
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
Cause
Solution
0x1700
Liquid volume too low for
vessel in location: ...
Total volume supplied by the  Calculate the total volume
for the source or
user in a source vessel is
destination vessel and
smaller than the volume
select a suitable vessel. For
needed for a sample transfer,
additional aspirated volume
reagent transfer or mix
with multidispense mode,
command (total volume =
refer to manual.
volume to be aspirated +
 Consider that the software
remaining volume for this
may calculate higher
vessel + (in case of
remaining volumes in some
multidispense mode:)
cases to avoid crashes.
additional aspirated volume.
 Set Detect volumes to off for
racks that are in park
positions at the beginning
of the procedure.
0x1701
Liquid volume too large for Total volume supplied in a
 Supply less volume in the
vessel in location:...
source vessel is larger than
vessel.
needed or larger than vessel.  Change application.
 When verifying the total
volume needed for the
source or destination, take
into account the additional
aspirated volume when
using multidispense mode.
epMotion 5070 only:
 Set Detect volumes to off for
racks that are in park
positions at the beginning
of the procedure.
0x1904
The following labware has Edit mode:
 Change the source or
been deleted: ...
The worktable was changed
destination in the parameter
after an application had been
of the respective command
programmed; thus, the
in order to match the
labware defined in a
worktable. In this case the
command is no longer
pattern also has to be
available.
re-edited.
 The labware has to be
reprogrammed in the
worktable.
0x1908
The method was written
See Error message.
with a newer program
structure. If you want to
edit this method, you need
to update your software.
 Update your software, or:
 Call local Eppendorf
Service.
137
138
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
Cause
0x190D
The following labware is
not selected in the
Labware File window: ...
Edit mode/worktable:
 Select the respective
The chosen labware is not
labware in the Labware File
available in the labware
window. You need to have
collection that had been
the appropriate user rights.
selected for your lab. Possible
If you do not have the
cause for this error message:
necessary user rights ask
The labware has been
your administrator.
deselected in the Labware File
window.
0x190E
 Select the respective
The following tool is not
Edit mode/procedure:
labware in the Labware File
selected in the labware file The chosen tool is not
window. You need to have
window: ...
available in the labware
the appropriate user rights.
collection that had been
If you do not have the
selected for your lab. Possible
necessary user rights ask
cause for this error message:
your administrator.
The labware has been
deselected in the Labware File
window.
0x1910
The position of the
following labware is not
available on this worktable.
The method was written
for another workstation
configuration.
Edit mode/worktable: The
chosen labware is not
available in the labware
collection that had been
selected for your lab in the
software node epMotion.
Possible cause for this error
message: The labware has
been deselected in the
epMotion node.
 Set the respective labware
The method was written
for another workstation
configuration.
The position of the
following labware is not
available on this worktable.
The position of required
labware is not available on
this device, e.g., you have a
5075 LH and the application
was written on a 5075 VAC.
 Load the concerned
The position of the
following labware is no
longer allowed
yyy
in location: xxx
Edit mode/worktable: The
 Place the respective
chosen labware may not be
labware on another
placed on the selected
position.
position anymore. Possible
cause for this error message:
The application has been
written with a former version
of the software.
0x1910
Solution
in the node epMotion.
application on a compatible
device, or
 Modify the application until
it matches the available
device.
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
Cause
0x1911
The following labware has Because you can change the  Either change the order or
contents of the Tubs +
been changed, so the
order or contents Tubs +
Modules(equip) + Holders
pattern no longer fits: xxx Modules(equip) + Holders
combination back to the
combination, the recent
original or change the
pattern of a command may
pattern in the command.
not fit the new positions of the
tubes.
0x1911
The following labware has Because the order or contents  Either change the order or
contents of the “Equipped
been changed, so the
of a “Equipped Holder”
Holder” combination back
pattern no longer fits: xxx combination can be changed,
to the original or change
the recent pattern of a
the pattern in the
command may not fit the new
command.
positions of the tubes.
0x1A02
The name is already used
for another labware
 Enter a different name.
Edit mode/labware:
The same name has been
defined for a different rack or
another labware item.
0x1A03
This position is not
available for the selected
labware
Edit mode/worktable:
 Place the selected labware
Certain worktable positions
in another location.
are not allowed for specific
labware (e.g., tips can only be
placed in the rear of the
worktable).
0x1A04
 See "Cause".
The selected labware may Edit mode/worktable:
not be stacked on top of
Building of labware stacks on
labware already placed
the worktable is restricted to
certain labware combinations
(e.g., thermorack above
thermorack does not make
sense).
0x1A05
Maximum number of
labware stacked: 5
0x1A06
 See "Cause".
Labware stack too high in Edit mode/worktable:
location: xxx
Labware stacks on the
Maximum height: xxx mm worktable may not exceed a
maximum height limit (e.g.,
plates on adapters is allowed;
reservoir holder on adapters
is not allowed because the
stack would be too high).
Edit mode/worktable:
Certain labware build a pile
which contains too many
when placed on certain
labware.
Solution
Place the selected labware in
another location.
139
140
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
0x1A10
Eight-channel dispensing Edit mode/parameter in
 Choose another rack or
tool cannot be used for this command Sample Transfer:
another tool.
source rack
Source rack does not fit the
eight-channel dispensing tool
(e.g., 24-well plate or tube
rack with 24 positions).
0x1A10
 Choose another rack or
Eight-channel dispensing Edit mode/parameter in
another tool.
tool cannot be used for this command Sample Transfer:
source rack
Source rack does not fit to
eight-channel dispensing tool
(e.g.: 24-well-plate or tube
rack with 24 positions).
0x1A11
 Choose another rack or
Eight-channel dispensing Edit mode/parameter in
another tool.
tool cannot be used for this command Sample Transfer:
destination rack.
Destination rack does not fit
the eight-channel dispensing
tool (e.g., 24-well plate or
tube rack with 24 positions).
0x1A12
No source or destination
selected
0x1A15
Invalid entry for movement Edit mode/parameter in
blow (0 ... 100)
transfer command:
A value beyond the allowed
range has been entered for
the parameter Movement
Blow.
 Enter a value between 0
Invalid entry for movement Edit mode/parameter in
blow (0 ... 100)
transfer command:
A value beyond the allowed
range has been entered for
the parameter Movement
Blow.
 Enter a value between 0
Invalid entry for delay blow Edit mode/parameter in
(0 ... 9999)
transfer command:
A value beyond the allowed
range has been entered for
the parameter Delay Blow.
 Enter a value between 0
Invalid entry for delay blow Edit mode/parameter in
(0 ... 9999)
transfer command:
A value beyond the allowed
range has been entered for
the Delay Blow parameter.
 Enter a value between 0
0x1A15
0x1A16
0x1A16
Cause
Solution
 Select source or
Edit mode/parameter in
destination, respectively.
command Sample Transfer:
Source or destination rack has
not been selected.
and 100%.
and 100%.
and 9999 msec.
and 9999 msec.
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
Cause
Solution
0x1A17
Invalid entry for speed
aspiration (0.2 ... 110)
Edit mode/parameter in
transfer command:
A value beyond the allowed
range has been entered for
the parameter Speed
Aspiration.
 Enter a value between 0.2
Invalid entry for speed
aspiration (0.2 ... 110)
Edit mode/parameter in
transfer command:
A value beyond the allowed
range has been entered for
the speed aspiration
parameter.
 Enter a value between 0.2
Invalid entry for speed
dispense (0.2 ... 110)
Edit mode/parameter in
transfer command:
A value beyond the allowed
range has been entered for
the parameter Speed
Dispense.
 Enter a value between 0.2
Invalid entry for speed
dispense (0.2 ... 110)
Edit mode/parameter in
transfer command:
A value beyond the allowed
range has been entered for
the parameter Speed
Dispense.
 Enter a value between 0.2
Invalid entry for speed
blow (0.2 ... 110)
Edit mode/parameter in
transfer command:
A value beyond the allowed
range has been entered for
the parameter Speed Blow.
 Enter a value between 0.2
Invalid entry for speed
blow (0.2 ... 110)
Edit mode/parameter in
transfer command:
A value beyond the allowed
range has been entered for
the parameter Speed Blow.
 Enter a value between 0.2
0x1A1A
Invalid entry for initial
stroke (0 ... 100)
Edit mode/parameter in
 Enter a value between 0
transfer command: a value
and 100%.
beyond the allowed range has
been entered for the
parameter initial stroke.
0x1A20
Eight-channel dispensing Edit mode/parameter in
 Choose another rack or
tool cannot be used for this command Reagent Transfer:
another tool.
source rack
Source rack does not fit the
eight-channel dispensing tool
(e.g., 24-well plate or tube
rack with 24 positions).
0x1A17
0x1A18
0x1A18
0x1A19
0x1A19
and 110 mm/sec.
and 110 mm/sec.
and 110 mm/sec.
and 110 mm/sec.
and 110 mm/sec.
and 110 mm/sec.
141
142
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
Cause
Solution
0x1A21,
0x1A30,
0x1A31,
0x1A51
Eight-channel dispensing Edit mode/parameter in the
 Choose another rack or
tool cannot be used for this affected command:
another tool.
destination rack
Destination rack does not fit
the eight-channel dispensing
tool (e.g., 24-well plate or
tube rack with 24 positions).
0x1A22,
0x1A32,
0x1A42,
0x1A52
No source or destination
selected
0x1A40,
0x1A41,
0x1A50
Eight-channel dispensing Edit mode/parameter in the
 Choose another rack or
tool tool cannot be used for affected command:
another tool.
this source rack
Source rack does not fit the
eight-channel dispensing tool
(e.g., 24-well plate or tube
rack with 24 positions).
0x1A61
 Choose another rack or
Eight-channel dispensing Edit mode/parameter in
another tool.
tool cannot be used for this command Mix:
rack
Rack does not fit the
eight-channel dispensing tool
(e.g., 24-well plate or tube
rack with 24 positions).
0x1A62
No rack selected
0x1A65
Invalid entry for speed (0.2 Edit mode/parameter in
... 110)
command Mix:
A value beyond the allowed
range has been entered for
the parameter Speed.
 Select source or
Edit mode/parameter in the
destination, respectively.
affected command:
Source or destination rack has
not been selected.
Edit mode/parameter in
command Mix:
Rack has not been selected.
 Select rack, respectively.
 Enter a value between 0.2
and 110 mm/sec.
Edit mode/pattern:
 Follow the direction of the
When editing the pattern you
edited pattern and move to
have tried to select a certain
a different position.
position that is already
occupied.
0x1A70
This position is already
occupied
0x1A76
eight-channel dispensing Edit mode/pattern:
 Choose another rack or
tool cannot be used for this Rack does not fit the
another tool.
ReservoirRack.
eight-channel dispensing tool
(e.g., Tubs + Modules (equip) +
Holders combination with
positions all less than 8 in
modules).
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
0x1A77
No ReservoirRack or tubes Edit mode/pattern:
found
Rack does not have any
positions (e.g., Tubs +
Modules (equip) +
Holders-combination with
positions all less than 1 in
Modules).
0x1A78
Number of tubes not
supported
0x1A80
Invalid entry for minutes (0 Edit mode/parameter in
... 99)
command Wait:
A value beyond the allowed
range has been entered for
the parameter minutes.
 Enter a value between 0
Invalid entry for seconds (0 Edit mode/parameter in
... 59)
command Wait:
A value beyond the allowed
range has been entered for
the parameter seconds.
 Enter a value between 0
0x1A81
Cause
Solution
 Choose another rack.
 Choose another rack.
Edit mode/pattern:
One or more Modules have 3,
5, 6, 7 or more than 8
positions. This is not
supported.
and 99 minutes.
and 59 seconds.
0x1A90
Selecting more than one
rack as Source or as
Destination:
All source racks (or all
destination racks, resp.)
must have the same well
pattern
 See explanation in "Cause".
Edit mode/parameter in
transfer command:
If more than one rack is
selected as source (or
destination) all source (or
destination), racks must have
the same well pattern (e.g.,
96-well plates).
0x1A90
Selecting more than one
rack as Source or as
Destination:
All source racks (or all
destination racks, resp.)
must have the same well
pattern
Edit mode/parameter in
transfer command:
The selected labware have a
different amount of wells.
 Choose another rack.
0x1AA0
Invalid entry for
temperature (0 ... 110)
Edit mode/parameter in
command Temperature:
A value beyond the allowed
range has been entered for
the parameter Temperature.
 Enter a value between 0
and 110 degrees.
143
144
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
Cause
Solution
0x1AE0
Invalid entry for mixing
speed (0 ... 2000)
Edit mode/parameter in
command Thermomixer: a
value beyond the allowed
range has been entered for
the parameter speed
Enter a value between 0 and
2000 rpm
0x1AE1
Invalid entry for minutes (0 Edit mode/parameter in
... 120)
command Thermomixer: a
value beyond the allowed
range has been entered for
the parameter minutes
Enter a value between 0 and
120 minutes
0x1AE2
Invalid entry for seconds (0 Edit mode/parameter in
... 59)
command Thermomixer: a
value beyond the allowed
range has been entered for
the parameter seconds
Enter a value between 0 and 59
seconds
0x1AE3
Invalid entry for
temperature (4 ... 110)
Edit mode/parameter in
command Thermomixer: a
value beyond the allowed
range has been entered for
the parameter temperature
Enter a value between 4 and
110 degrees
0x1C0B
Sample number too large
Run mode:
 Start the application again
The number of samples you
and enter a lower number
entered will fill more than one
of samples; or:
 Enter the edit mode and
rack (source or destination,
program a pattern that
respectively) based on the
together with the number of
programmed pattern.
samples you want to run
will not extend beyond one
rack.
0x1C0D
 Delete the old pattern by
You must clear old pattern Edit mode/pattern:
pressing the New pat.
first
You tried to change a stored
softkey
Press new pattern
pattern before deleting the old
pattern.
0x1C0D
You must clear old pattern Edit mode/pattern:
 Delete the old pattern by
first
You tried to change a stored
pressing the button new
Press "new pattern"
pattern before deleting the old
pattern.
pattern.
0x1C0E
You must go forward
Edit mode/pattern:
 See explanation in "Cause".
When entering the pattern,
the order of edited locations
in the source (or destination,
respectively) must be from left
to right or from the top of the
pattern downwards (i.e., move
only in columns or in rows).
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
Cause
Solution
0x1C0F
You may only move
horizontally or vertically
 See explanation in "Cause".
Edit mode/pattern:
When entering the pattern the
order of edited locations in
the source (or destination,
resp.) must be from the left to
right or from top of the
pattern downwards (i.e., move
only in columns or in rows).
Note: Error message may also
occur when working with an
eight-channel dispensing tool
and editing another position
than the upper ones (see error
code 0x1C1F).
0x1C10
Pattern for replicates of
first sample too complex
Edit mode/pattern:
 Enter a simpler pattern if
The pattern algorithm cannot
possible.
handle this pattern.
In case this is not possible:
 Call local Eppendorf
Application Support.
Edit mode/pattern:
 Enter a simpler pattern if
The pattern algorithm cannot
possible.
handle this pattern.
In case this is not possible:
Note: See note in error
 Call local Eppendorf
0x1C0F.
Application Support.
0x1C11
Pattern too complex
0x1C14
Pattern must fit in rows or Edit mode/pattern:
 Enter a simpler pattern if
columns
The basic unit of the pattern
possible.
you tried to enter extends
In case this is not possible:
beyond a row or a column.
 Call local Eppendorf
This cannot be handled by the
Application Support.
pattern algorithm.
0x1C15
Pattern too complex
 Enter a simpler pattern if
Edit mode/pattern:
possible.
The pattern algorithm cannot
In case this is not possible:
handle this pattern.
Note: See note in error
 Call local Eppendorf
0x1C0F.
Service.
0x1C16
This position is already
occupied
Edit mode/pattern:
 Following the edited
When editing the pattern you
pattern move to a different
have tried to select a certain
rack position.
rack position that is already
occupied.
0x1C17
You must start with the
source
Edit mode/pattern:
When editing a pattern you
must start with the source.
 See explanation in "Cause".
145
146
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
0x1C18
Please enter a source now Edit mode/pattern:
 Enter the same number of
In the destination rack, you
replicates for all sources
tried to enter more replicates
you edit when
than you had sources.
programming a pattern.
0x1C19
Please enter a destination Edit mode/pattern:
 Press the Destination
now
When having selected a
softkey and edit the
source in the Sample Transfer
destination position(s) for
command you first have to
the selected source
enter a destination for this
position.
source before moving to the
next source position.
0x1C1A
No more positions
available (limited by
Number of Samples
command)
 Select a pattern that fits the
Edit mode/pattern:
programmed Number of
Editing further positions is
Samples command.
not possible because the limit
set in the Number of Samples
command would be exceeded.
0x1C1E
Pattern for reagent
transfer:
Source can be chosen only
once
Edit mode/pattern for
command Reagent Transfer:
After having entered the
source and the destinations
for the reagent transfer you
cannot select an additional
source.
0x1C1F
Pattern with eight-channel
dispensing tool:
Please edit upper position
of this tool
Edit mode/pattern with
 See explanation in "Cause".
eight-channel dispensing tool:
Only the upper positions of
the eight-channel dispensing
tool can be selected.
0x1C20
Pattern for sample transfer: Edit mode/pattern for
only one position per
command Sample Transfer:
sample on source
Before selecting a second
source position, you have to
edit the destination for the
first source position.
 Enter destination for the
In source rack further
positions cannot be edited
because positions in
destination rack are
already occupied
 Edit a pattern that does not
0x1C21
Cause
Edit mode/pattern:
Selecting further source
positions would require a
second destination rack
according to the pattern you
edited.
Solution
 Enter the source only once.
In case this does not meet
your requirements for this
application consider
selecting command Sample
Transfer instead of Reagent
Transfer, or:
 Call local Eppendorf
Application Support.
source you just selected;
afterwards, you can edit the
next source position.
require more than one
destination rack per
command. To use more
destination racks, create
additional commands.
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
0x1C22
Pattern for pool one dest: Edit mode/pattern for
destination can be chosen command Pool one
only once
destination:
After having entered the
sources and the destination,
you cannot select an
additional destination.
 Enter the destination only
Pattern for dilute:
only one position per
sample on source
Edit mode/pattern for
command Dilute:
Before selecting a second
source position, you have to
edit the destination for the
first source position.
 Enter destination for the
0x1C25
Pattern for pool:
only one position per
sample on destination
 Enter sources for the next
Edit mode/pattern for
destination position;
command Pool:
afterwards, you can edit the
Before selecting a second
next destination position.
destination position, you have
to edit the next source
positions to be pooled into
this destination.
0x1C26
Pattern for Reagent
Transfer: not enough
source positions
Run mode: To provide enough  Start the application again
and enter a lower number
reagent volume for the
of samples; or:
number of samples you
entered, the selected reagent  Enter the edit mode and
program more reagent
source positions must be
source positions in the
higher.
pattern. Keep in mind that
the selected reagent source
positions may not extend
beyond one rack.
0x2025
Bottom tolerance too large Bottom tolerance too big.
 Use a smaller value.
0x2026
Bottom tolerance too small Bottom tolerance too small.
 Use a bigger value.
0x2101
Tool not defined.
Parameter Pipet. tool was not  See explanation in "Cause".
edited in the application.
0x2104
Tips not edited in
worktable/procedure
Tips were edited in the
 Edit the tips that you
procedure of the application,
programmed in the
but they were not edited in
procedure in the worktable.
the worktable (e.g., filter tips
<-> tips without filter).
0x1C23
Cause
Solution
once. In case this does not
meet your requirements for
this application, consider
selecting command Pool
instead of Pool one
destination, or:
 Call local Eppendorf
Application Support.
source you just selected;
afterwards, you can edit the
next source position.
147
148
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
Cause
Solution
0x2105
Parameter conflict:
Start volume greater than
filling volume of source
tube or well
The Volume and Source
parameters of the source
vessel do not match (Volume
is higher than the maximum
filling volume of the source
vessel).
 Edit Volume and Source in
0x2106
Parameter conflict: Start
volume greater than filling
volume of destination
vessel or well.
The Volume and Destination  Edit Volume and Destination
in the worktable so that
parameters of the destination
Volume is covered by the
vessel do not match (Volume
maximum filling volume of
is higher than the maximum
the destination vessel.
filling volume of the
destination vessel).
0x2107
Volume not defined
Parameter Volume was not
edited in the application.
0x2108
Parameter conflict:
Volume too small for this
tool
The parameters Volume and  Edit Volume and Pipet. tool
Pipet. tool of the application
so that Volume is covered
do not match (Volume is
by the volume range of the
smaller than the lower limit of
pipet tool.
the tool volume range).
0x2109
Parameter conflict:
Volume too large for this
tool
The parameters Volume and  Edit Volume and Pipet. tool
Pipet. tool of the application
so that Volume is covered
do not match (Volume is
by the volume range of the
higher than the upper limit of
pipet tool.
the tool volume range).
0x210A
Parameter conflict: volume The Volume and Source
 Edit Volume and Source so
greater than filling volume parameters of the application
that Volume is covered by
of source tube or well
do not match (Volume is
the maximum filling volume
higher than the maximum
of the source vessel.
filling volume of the source
vessel).
0x210B
Parameter conflict:
volume greater than filling
volume of destination
vessel or well.
The Volume and Destination  Edit Volume and Destination
so that Volume is covered
parameters of the application
by the maximum filling
do not match (Volume is
volume of the destination
higher than the maximum
vessel.
filling volume of the
destination vessel).
0x210D
Source rack not defined
Parameter Source was not
edited in the application.
0x210E
Source rack not edited in
worktable
The source rack you edited in  Edit the rack that you
the procedure of the
programmed in the
application has been removed
procedure as Source in the
from the worktable.
worktable, or edit a
different source rack in the
application.
the worktable so that
Volume is covered by the
maximum filling volume of
the source vessel.
 See explanation in "Cause".
 See explanation in "Cause".
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
Cause
0x210F
Source rack not selected in The source rack you edited in  Select the rack in the
labware file window or edit
the labware file window
the application is not selected
a different rack in the
or removed in the labware file
application.
window and therefore is not
available for programming.
0x2110
Destination rack not
defined
0x2111
Destination rack not edited Destination rack was edited in  Edit the rack that you
in worktable
the procedure of the
programmed in the
application, but it was not
procedure as Destination in
edited in the worktable.
the worktable or edit a
different destination rack in
the application.
0x2112
Destination rack not
selected in the Labware
File Window
 Select the rack in the
The destination rack you
labware file window or edit
edited in the application is not
a different rack in the
selected or removed in the
application.
labware file window and
therefore is not available for
programming.
0x2113
Pattern not defined
Parameter Pattern was not
edited in the application.
0x2114
Loading error (invalid
entry in pattern)
Normally a system error; but  Edit a pattern with source
may also be caused by editing
and destination positions.
a pattern without destination In other cases:
positions; or:
 Call local Eppendorf
File damaged.
Service.
0x211B
Liquid type not defined
Parameter Liquid Type was
 Choose a liquid type.
not edited in the application.
0x211D
Mixing cycles in source not Parameter No. of Cycles in a  See explanation in "Cause".
defined
mix procedure was not edited
for the source in the
application (mix procedure as
defined in a command Mix or
as part of a liquid transfer
command via parameter
Options).
0x211E
Invalid entry for mixing
cycles in source (1 ... 99)
Parameter Source was not
edited in the application.
Solution
 See explanation in "Cause".
 See explanation in "Cause".
 Enter a number between 1
Entry for the No. of Cycles
and 99 for the No. of Cycles
parameter in a mix procedure
parameter.
for source vessels was higher
than the max. limit (1 up to 99
cycles) (mix procedure as
defined in a command Mix or
as part of a liquid transfer
command via parameter
Options).
149
150
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
Cause
Solution
0x211F
Invalid entry for mixing
speed in source (1 ... 10)
Entry for the parameter Speed  Enter a number between 1
in a mix procedure for source
and 10 for the Speed
vessels was higher than the
parameter.
max. limit (1 up to 10) (mix
procedure as defined in a
command Mix or as part of a
liquid transfer command via
parameter Options).
0x2120
Mixing volume in source
not defined
 See explanation in "Cause".
Parameter Volume in a mix
procedure for source vessels
was not edited in the
application (mix procedure as
defined in a command Mix or
as part of a liquid transfer
command via parameter
Options).
0x2121
Parameter conflict: mixing The parameters Volume and  Edit Volume and Pipet. tool
so that Volume is within
volume in source too large Pipet. tool of a mix procedure
pipet tool's volume range.
for this tool
for source vessels are not in
agreement (Volume is higher
than the upper limit of the
tool's volume range) (mix
procedure as defined in a
command Mix or as part of a
liquid transfer command via
parameter Options).
0x2122
Parameter conflict: mixing The parameters Volume and  Edit Volume and Pipet. tool
so that Volume is within the
volume in source too small Pipet. tool of a mix procedure
pipet tool's volume range.
for this tool
for source vessels do not
match (Volume is less than the
lower limit of the tool's
volume range) (mix procedure
as defined in a command Mix
or as part of a liquid transfer
command via parameter
Options).
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
Cause
Solution
0x2123
Parameter conflict:
mixing volume in source
greater than filling volume
of source tube or well
 Edit Volume and Source so
The Volume and Source
that Volume is within the
parameters of a mix
allowable filling volume of
procedure in the application
the source vessel.
do not match (Volume is
higher than the maximum
filling volume of the source
vessel) (mix procedure as
defined in a command Mix or
as part of a liquid transfer
command via parameter
Options).
0x2124
Mixing cycles in
destination not defined
Parameter No. of Cycles in a  See explanation in "Cause".
mix procedure for destination
vessels was not edited in the
application (mix procedure as
part of a liquid transfer
command via parameter
Options)
0x2125
Invalid entry for mixing
cycles in destination
(1 ... 99)
Entry for the parameter No. of  Enter a number between 1
and 99 for the No. of Cycles
Cycles in a mix procedure for
parameter.
destination vessels was higher
than the max. limit (1 up to
99 cycles) (mix procedure as
part of a liquid transfer
command via parameter
Options).
0x2126
Invalid entry for mixing
speed in destination
(1 ... 10)
Entry for the parameter Speed  Enter a number between 1
and 10 for the Speed
in a mix procedure for
parameter.
destination vessels was higher
than the max. limit (1 up
to 10) (mix procedure as part
of a liquid transfer command
via parameter Options).
0x2127
Mixing volume in
destination not defined
 See explanation in "Cause".
Parameter Volume in a mix
procedure for destination
vessels was not edited in the
application (mix procedure as
part of a liquid transfer
command via parameter
Options).
151
152
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
Cause
Solution
0x2128
Parameter conflict:
mixing volume in
destination too large for
this tool
The parameters Volume and  Edit Volume and Pipet. tool
Pipet. tool in a mix procedure
so that Volume is within the
for destination vessels do not
pipet tool's volume range.
match (Volume is higher than
the upper limit of the tool
volume range) (mix procedure
as part of a liquid transfer
command via parameter
Options).
0x2129
Parameter conflict: mixing The parameters Volume and  Edit Volume and Pipet. tool
so that Volume is within the
volume in destination too Pipet. tool in a mix procedure
volume range of the pipet
small for this tool
for destination vessels do not
tool.
match (Volume is lower than
the minimum allowed volume)
(mix procedure as part of a
liquid transfer command via
parameter Options).
0x212A
Parameter conflict: mixing
volume in destination
greater than filling volume
of destination vessel or
well.
The Volume and Destination  Edit Volume and Destination
so that Volume is within the
parameters in a mix
maximum filling volume of
procedure for destination
the destination vessel.
vessels do not match (Volume
is higher than the maximum
filling volume of the
destination vessel) (mix
procedure as part of a liquid
transfer command via
parameter Options).
0x212C
Parameter conflict:
mix after dispense not
allowed in multidispense
mode
 Change parameter transfer
When the transfer type
type to pipette, or:
parameter is set to
 Omit the mixing step; in
multidispense, the mix after
this case you could also edit
dispense parameter cannot be
another mixing step as a
edited for this command.
new command (Mix), which
would be performed after
the previous command of
the procedure has ended.
0x212D
Parameter conflict:
eight-channel dispensing
tool cannot be used for this
source rack
Edit mode/parameter in liquid  Choose another rack or
another tool.
handling command:
Source rack does not fit the
eight-channel dispensing tool
(e.g., 24-well plate or tube
rack with 24 positions).
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
Cause
Solution
0x212E
Parameter conflict:
eight-channel dispensing
tool cannot be used for this
destination rack
Edit mode/parameter in liquid  Choose another rack or
another tool.
handling command:
Destination rack does not fit
the eight-channel dispensing
tool (e.g., 24-well plate or
tube rack with 24 positions).
0x212F
Sample number too large
The number of samples you
have entered will fill more
than one rack (source or
destination, respectively)
based on the programmed
pattern.
 Program a pattern that
together with the number of
samples you want to run
will not extend beyond one
rack, or choose a lower
number of samples in the
corresponding Number of
Samples command.
0x2130
Parameter conflict: tip
cannot be used for this
source rack
The Source rack parameter in  Choose another rack or
another tool.
the liquid handling command
does not match the selected
tool (e.g., 384-well plate and
TS_1000 or TM1000_8).
0x2131
Parameter conflict: tip
cannot be used for this
destination rack
The Destination rack
 Choose another rack or
parameter in the liquid
another tool.
handling command does not
match the selected tool (e.g.,
384-well plate and TS_1000
or TM1000_8).
0x2132
Invalid number of samples Number of samples you have  Enter a maximum number
of samples up to 384.
(1 ... 384)
entered is too high.
0x2133
Rack or plate not defined
Edit mode/parameter in
command Transport:
Labware to be transported
was not edited.
 See explanation in "Cause".
0x2134
Rack not selected in the
labware file window
The rack you edited in a
command Transport
(parameter labware) is not
selected or removed in the
labware file window and
therefore is not available for
programming.
 Select the rack in the
Location for destination
not defined
 See explanation in "Cause".
Edit mode/parameter in
command Transport:
The destination position for
the labware to be transported
was not edited.
0x2135
labware file window or edit
a different rack in the
application.
153
154
epBlue - Software error messages
epBlue™ with EasyCon
English (EN)
Code
Problem
Cause
Solution
0x2136
Invalid entry for seconds
(1 ... 59)
Edit mode/parameter in
command Wait:
A value beyond the allowed
range has been entered for
the seconds parameter.
 Enter a value between 0
Invalid entry for minutes
(1 ... 99)
Edit mode/parameter in
command Wait:
A value above the allowable
maximum minutes has been
entered for the minutes
parameter.
 Enter a value between 0
Method without active
commands
Application only contains
passive commands (such as
wait, comment, etc.).
 Insert at least one active
0x2139
Parameter conflict
mix before aspirating is not
allowed in multiaspirate
mode.
Commands Pool and Pool One  Do not mix.
Destination:
Mix before aspirating not
allowed in multiaspirate
mode.
0x213A
Labware to be exchanged Parameter in command
 Enter a new labware for one
are identical
Exchange:
of the two positions.
Both values point to the same
labware.
0x213A
Labware to be exchanged Parameter in command
are identical
Exchange: Both values point
to the same labware.
0x2137
0x2138
and 59 seconds.
and 99 minutes.
command.
 Enter a new labware for one
of the two positions.
0x2170
Parameter conflict:
To edit the elution from filter  See explanations in
Parameter elution from
option a filter plate must have
"Cause".
filter is only possible when been edited as source.
filter plates have been
selected as source
0x2171
Parameter conflict:
See explanation in the error
Multidispense mode is not message.
allowed when elution from
filter is selected
0x2172
Parameter conflict:
Transfer volume must be
set to zero when the elution
from filter parameter has
been selected
 See explanation in the error
message.
Using the elution from filter
 Set the volume to zero
option, the complete volume
because the entry will not
contained in the filter plate
have an effect in the
wells is always aspirated;
application run.
therefore, editing a volume to
be transferred is not possible.
Ordering information
epBlue™ with EasyCon
English (EN)
13
13.1
Ordering information
Accessories
Order no.
(International)
5075 002.507
5075 002.515
5075 002.523
Description
LH assistant
PCR assistant
designed to streamline and automate the whole PCR set-up workflow, like Compose
Mastermix, Normalize Concentrations, Create Dilution Series, Setup Reactions
Prep assistant
provide a step-by-step guided workflow of automated nucleic acid purification with
the MagSep reagent kits
155
156
Index
epBlue™ with EasyCon
English (EN)
Index
Pool one destination ......................................... 61
Reagent transfer ............................................... 57
Sample transfer ................................................ 47
Temperature..................................................... 67
ThermoMixer ................................................... 68
Transport.......................................................... 70
User intervention .............................................. 71
Wait ................................................................. 73
A
Application
Change name ...................................................85
Change properties............................................85
Control .............................................................31
Copy .................................................................84
Creation............................................................35
Delete ...............................................................84
Entering the number of samples......................25
Export...............................................................87
Filling volume of the labware...........................28
Home................................................................30
Import ..............................................................86
Labware run parameters..................................27
Manage ............................................................83
Open.................................................................23
Preparation.......................................................24
Run parameters for barcode reader...........26, 27
Run parameters for optical sensor...................26
Saving as PDF ..................................................88
Saving for a different epMotion model ............89
Setting the HEPA air filter and the UV lamp....25
Assistants...............................................................15
Avoiding the formation of foam.............................53
B
Backup.................................................................118
Creation..........................................................118
Bottom tolerance
Setting ..............................................................96
C
D
Data backup ........................................................ 118
Dilution series
Create ........................................................ 58, 59
Example........................................................... 59
E
EasyCon tablet
Switching off.................................................... 18
epBlue
Exiting ............................................................. 18
Menu bar ........................................................ 83
Start ................................................................. 17
Start screen ..................................................... 83
Eppendorf ThermoMixer module
Maximum speeds .................................... 69, 122
External process ................................................... 72
F
Features ................................................................ 15
File menu .............................................................. 19
Folder
Manage............................................................ 83
Changing the liquid type .......................................55
Commands
Comment ..........................................................64
Dilute ................................................................58
Exchange ..........................................................64
External process ..............................................72
Magnetic separation ........................................65
Mix ...................................................................66
Number of samples...........................................66
Pierce ...............................................................62
Pool ..................................................................61
H
HEPA air filter ..................................................... 113
Home ............................................................. 113
Stop ............................................................... 114
L
Labware
Activate............................................................ 93
Deactivate ........................................................ 93
Index
epBlue™ with EasyCon
English (EN)
Delete ...............................................................93
Detect volumes of the optical sensor ..........78, 79
Export...............................................................95
Import ..............................................................93
Labware combinations .....................................97
Labware definition .................................103, 104
Labware library ..............................................103
Manage ............................................................92
Maximum speeds .....................................69, 122
Naming conventions ........................................91
Placement ........................................................37
Positioning on the worktable ...........................26
Properties.........................................................95
Run parameters for the optical sensor.............80
Setting the bottom tolerance ...........................96
Special features..............................................104
Stack.................................................................38
Detect volumes for special labware.................. 79
Measure filling volume .................................... 77
Run parameters for labware ............................ 80
P
Pattern
Definition ......................................................... 73
Irregular pattern .............................................. 76
Regular pattern................................................ 74
PDF ....................................................................... 88
Pierce .................................................................... 62
Pipette tips
Rinse................................................................ 52
Print templates.................................................... 116
Labware combinations
Change .............................................................97
Create ...............................................................97
Procedure
Processing ....................................................... 43
Labware definition
Loading from the Internet..............................103
Request from Eppendorf ................................103
Special features..............................................104
R
LH assistant ...........................................................15
Liquid types ...........................................................53
Log
Print .................................................................34
Saving as PDF ..................................................34
Rack
Load............................................................... 100
Report
Display............................................................. 33
ReservoirRack
Equipping with ReservoirRack modules ....... 102
Equipping with Reservoirs ............................ 102
ReservoirRack module
Equipped with individual vessels .................. 100
M
Magnetic separation..............................................65
S
Menu bar ...............................................................83
Screen keyboard ................................................... 18
Mixing ...................................................................66
Start screen..................................................... 17, 83
Mouse....................................................................18
T
O
Optical sensor........................................................77
Basic settings .................................................121
Check labware placement ................................81
Detect tips ........................................................81
Detect volumes..................................................77
Detect volumes application parameter for plates
.........................................................................78
Thermorack
Load............................................................... 100
Touchscreen.......................................................... 18
U
UV
Lamp.............................................................. 112
157
158
Index
epBlue™ with EasyCon
English (EN)
W
Worktable
Decontamination............................................112
Positioning labware..........................................26
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