epBlue™ with MultiCon

epBlue™ with MultiCon
hmanual
ual
MultiCon
EN)
epBlue™ with MultiCon
Software manual
Software version 40.4
Copyright© 2015 Eppendorf AG. All rights reserved, including graphics and images. No part of this
publication may be reproduced without the prior permission of the copyright owner.
Focus Diagnostic® is a registered trademark of Focus Diagnostic, Inc., USA.
Qiagen® and Rotor-Disk® are registered trademarks of Qiagen GmbH, Germany.
Excel® and Windows® are registered trademarks of Microsoft Corporation in the United States and other
countries.
epMotion®, Eppendorf Tubes ®, epT.I.P.S.®, and Eppendorf ThermoMixer® are registered trademarks of
Eppendorf AG, Germany.
Eppendorf® and the Eppendorf logo are registered trademarks of Eppendorf AG, Germany.
Registered trademarks and protected trademarks are not marked in all cases with ® or ™ in this manual.
The software of the device (firmware) contains open source software. License information is available on
request from Eppendorf AG.
Only for epMotion M5073 and epMotion 5075m: Limited Use Label License NOTICE TO PURCHASER;
LIMITED LICENSE FOR RESEARCH USE ONLY.
This product and its use may be covered by one or more patents owned by Gen-Probe Incorporated. The
purchase price for this product includes only limited, nontransferable rights under certain claims of certain
patents owned by Gen-Probe Incorporated to use this product for research purposes only. No other rights
are conveyed. Purchaser is not granted any rights under patents of Gen-Probe Incorporated to use this
product for any commercial use. Further information regarding purchasing a license under patents of
Gen-Probe Incorporated to use this product for any other purposes, including, without limitation, for
commercial use, may be obtained by contacting Gen-Probe Incorporated, Attn: Business Development
Department, 10210 Genetic Center Drive, San Diego, California 92121-4362, U.S.A.
5075 900.866-02/062015
Table of contents
epBlue™ with MultiCon
English (EN)
Table of contents
1
Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1.1
Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1.2
Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1.2.1
Danger symbols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1.2.2
Danger levels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1.3
Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
1.4
Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
1.5
Glossary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
1.6
Version overview for the epBlue with MultiCon software manual . . . . . . . . . . . . . . . . . . . . . . . 14
2
Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3
Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
3.1
Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
3.1.1
epBlue . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
3.1.2
Assistants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
4
Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4.1
Starting and exiting epBlue . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4.1.1
Starting epBlue . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4.1.2
Starting epBlue for the first time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4.1.3
Logging in as user. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4.1.4
Logging off as a user. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
4.1.5
Ending epBlue and switching off the operating unit . . . . . . . . . . . . . . . . . . . . . . . . . . 20
4.2
Initial steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
4.2.1
Using touchscreen and mouse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
4.2.2
Using the screen keyboard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
4.2.3
File menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4.2.4
Status area. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
4.2.5
Enlarging the view . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5
epBlue Application Runner - Carrying out the application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5.1
Opening an application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5.2
Positioning the labware on the epMotion worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5.3
Preparing the application run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
5.3.1
Selecting the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
5.3.2
Entering the number of samples. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.3.3
Setting the HEPA air filter and the UV lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.3.4
Positioning the labware on the epMotion worktable . . . . . . . . . . . . . . . . . . . . . . . . . . 28
5.3.5
Setting the run parameters for the optical sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
5.3.6
Setting the run parameters for the labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
5.3.7
Setting the ID tracking parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
5.3.8
Entering the filling volume of the labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.4
Starting the application. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
5.5
Controlling the application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
5.6
Ending the application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
3
4
Table of contents
epBlue™ with MultiCon
English (EN)
6
epBlue Log viewer - Managing application logs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
6.1
Displaying protocols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
6.2
Saving the log as a PDF file . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
7
epBlue Application Editor - Creating and editing applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
7.1
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
7.2
Creating an application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
7.2.1
Menu bar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
7.2.2
Creating an empty application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
7.2.3
Copying applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
7.2.4
Saving applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
7.2.5
Positioning labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
7.2.6
Defining the application parameters of the labware. . . . . . . . . . . . . . . . . . . . . . . . . . . 46
7.2.7
Defining procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
7.2.8
Checking an application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
7.3
Commands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
7.3.1
Sample transfer command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
7.3.2
Reagent transfer command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
7.3.3
Dilute command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
7.3.4
Pool command. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
7.3.5
Pool one destination command. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
7.3.6
Pierce command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
7.3.7
Comment command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
7.3.8
Exchange command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
7.3.9
Magnetic separation command. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
7.3.10 Mix command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
7.3.11 Number of samples command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
7.3.12 Temperature command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
7.3.13 ThermoMixer command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
7.3.14 Vacuum command. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
7.3.15 Transport command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
7.3.16 User intervention command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
7.3.17 External process command. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
7.3.18 Wait command . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
7.4
Working with CSV files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
7.4.1
Creating CSV files for liquid transfer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
7.4.2
Importing a CSV file for liquid transfer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
7.4.3
Creating CSV files for the filling volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
7.4.4
Importing CSV files for the filling volume. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
7.5
Pattern . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
7.5.1
Menu bar in the Edit Pattern window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
7.5.2
Defining regular patterns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
7.5.3
Defining irregular patterns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
7.6
Optical sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
7.6.1
Measuring the filling level of the labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
7.6.2
Detecting pipette tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
7.6.3
Checking the labware on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
Table of contents
epBlue™ with MultiCon
English (EN)
8
epBlue Application Editor - Managing applications and folders. . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
8.1
Menu bar and start screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
8.2
Copying and deleting applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
8.2.1
Copying applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
8.2.2
Deleting an application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
8.3
Changing the properties and name of the application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
8.4
Importing and exporting applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
8.4.1
Importing applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
8.4.2
Exporting applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102
8.5
Saving an application as a PDF file. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
9
epBlue Labware Editor - Creating and managing labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
9.1
Overview of labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
9.2
Managing labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106
9.2.1
Menu bar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106
9.2.2
Deactivating and activating labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
9.2.3
Deleting labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
9.2.4
Importing labware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
9.2.5
Exporting labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
9.2.6
Viewing the properties of the labware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
9.2.7
Setting the bottom tolerance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110
9.3
Creating labware combinations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
9.3.1
Creating a labware combination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
9.3.2
Creating a labware combination based on existing labware . . . . . . . . . . . . . . . . . . . 112
9.3.3
Equipping racks, thermoracks, ReservoirRack modules with individual vessels . . . . 114
9.3.4
Equipping the ReservoirRack with reservoirs and ReservoirRack modules . . . . . . . . 116
9.4
Labware library . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
9.4.1
Loading labware definitions from the Internet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
9.4.2
Request labware definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
9.4.3
Add a labware definition to a labware library . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
9.5
Special labware features in epBlue . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118
9.5.1
Location B0 on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118
9.5.2
Tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118
9.5.3
MTP plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118
9.5.4
PCR plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118
9.5.5
Filter plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118
9.5.6
epT.I.P.S. Motion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119
9.5.7
Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119
9.5.8
Rack 96 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119
9.5.9
Rack LC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119
9.5.10 ReservoirRack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119
9.5.11 ReservoirRack Module TC. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120
9.5.12 Thermoracks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120
9.5.13 Thermorack Rotor/Tubes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
9.5.14 TipHolder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
9.5.15 Thermoadapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
9.5.16 Universal Disc by Focus Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
9.5.17 Thermoblocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
9.5.18 Thermoblock PCR 96 OC. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
5
6
Table of contents
epBlue™ with MultiCon
English (EN)
10 epBlue Application Simulator - simulating applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
10.1 Simulating the application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
10.1.1 Starting a simulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
10.1.2 Controlling the simulation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124
11 epBlue ID - Working with ID tracking. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125
11.1 Scanning IDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125
11.2 ID lists . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125
11.2.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125
11.2.2 Input lists and result lists . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 126
11.2.3 Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127
11.2.4 Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127
11.3 Commands for working with IDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127
11.4 Reading IDs into epBlue . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127
11.5 Managing ID lists . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128
11.5.1 Displaying ID lists . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128
11.5.2 Filtering and sorting ID lists . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129
11.5.3 Importing ID lists . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129
11.5.4 Exporting ID lists . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130
11.5.5 Deleting ID lists. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130
11.6 Making the basic settings for the epBlue ID. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131
11.6.1 Making the basic settings for the epBlue ID. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131
12 epBlue settings - Checking components and making the basic settings . . . . . . . . . . . . . . . . . . . . 133
12.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133
12.2 Levelsensor options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133
12.3 Tool interlock. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134
12.3.1 Lock the tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134
12.3.2 Unlocking the tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134
12.4 Dosing device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
12.4.1 Reading out the tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
12.5 Gripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
12.5.1 Moving the gripper arms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 136
12.6 Temperature control unit X . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 136
12.7 ThermoMixer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 137
12.7.1 Checking the mixing function of the Eppendorf ThermoMixer module. . . . . . . . . . . 138
12.7.2 Checking the temperature control of the Eppendorf ThermoMixer module . . . . . . . 138
12.8 Magnetic separation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139
12.9 UV lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 140
12.9.1 Decontaminating the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 140
12.10 HEPA air filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 141
12.10.1 Switching on the HEPA air filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 141
12.10.2 Switching off the HEPA air filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 142
12.11 Setting the barcode reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 142
Table of contents
epBlue™ with MultiCon
English (EN)
13 epBlue settings - Creating and managing users. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 143
13.1 Managing the user accounts as an administrator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 143
13.1.1 Logging in as administrator in epBlue . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 143
13.1.2 epBlue user groups . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144
13.1.3 User accounts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 145
13.1.4 Assigning a new password for a user account . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148
13.1.5 Creating and validating checksums . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148
13.2 Managing your own user account. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151
13.2.1 Signing into and out of the user account . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151
13.2.2 Changing the password of your own user account. . . . . . . . . . . . . . . . . . . . . . . . . . . 151
13.2.3 Activating warning messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 152
14 epBlue Settings - Saving data and creating print templates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153
14.1 Data backup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153
14.1.1 Backing up the database . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154
14.1.2 Creating an automatic backup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155
14.1.3 Restoring the database . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155
14.1.4 Importing a database from epBlue version 40.X . . . . . . . . . . . . . . . . . . . . . . . . . . . . 156
14.2 Changing the device name . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157
14.3 Print templates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 158
14.3.1 Creating a print template . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 159
14.3.2 Saving print templates as default . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 159
14.3.3 Deleting a print template . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 159
15 epBlue System log viewer - managing database logs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 161
15.1 Audit trail . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 161
15.1.1 Opening the audit trail . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 161
15.1.2 Displaying the details of an entry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 161
15.1.3 Sorting entries. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 162
15.1.4 Creating a filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 162
15.1.5 Editing a filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 162
15.1.6 Selecting a filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 162
15.1.7 Deleting a filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 162
15.1.8 Selecting columns to be displayed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 162
15.1.9 Printing and exporting the audit trail . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 163
15.1.10 Audit trail categories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 163
16 epBlue - Software error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 167
16.1 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 167
17 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 195
17.1 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 195
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 196
7
8
Table of contents
epBlue™ with MultiCon
English (EN)
Operating instructions
epBlue™ with MultiCon
English (EN)
1
1.1
Operating instructions
Using this manual
Your epMotion operating manual consists of hardware instructions and software instructions. Separate
instructions exist for some optional software modules.
The operating manual is part of the product.
The current version of the operating manual can be found on our website: www.eppendorf.com.
 Read the operating manual in full before using the device.
 Store the operating manual at an easily accessible location.
 The device may only be transferred with the operating manual.
 If the operating manual is lost, replace it immediately. Please contact Eppendorf AG for further details.
1.2
Danger symbols and danger levels
1.2.1
Danger symbols
The safety instructions in this manual appear with the following danger symbols and danger levels:
1.2.2
Biohazard
Explosive substances
Strong magnetic field
UV radiation
Electric shock
Heavy load
Cuts
Hot surface
Hazard point
Material damage
Danger levels
DANGER
Will lead to severe injuries or death.
WARNING
May lead to severe injuries or death.
CAUTION
May lead to light to moderate injuries.
NOTICE
May lead to material damage.
9
10
Operating instructions
epBlue™ with MultiCon
English (EN)
1.3
Symbols used
Depiction
Meaning
1.
2.
Actions in the specified order

Actions without a specified order
•
List
Text
Display text or software text
Additional information
1.4
Abbreviations used
BCR
Barcode reader
CSV
Comma Separated Value
DNA
Deoxyribonucleic acid
DWP
Deepwell plate
epT.I.P.S.
Eppendorf Totally Integrated Pipetting System
HEPA
High Efficiency Particulate Air (filter)
LH
Liquid Handling
LIMS
Laboratory Information Management System
MTP
Microplate
PCR
Polymerase chain reaction
PDF
Portable Document Format
TMX
Eppendorf ThermoMixer module
USB
Universal Serial Bus
UV
Ultraviolet radiation
VAC
Vacuum unit
Operating instructions
epBlue™ with MultiCon
English (EN)
1.5
Glossary
A
Administrator
User possessing the rights for the administration of epBlue.
Application
Program for a dedicated application. An application comprises the procedure and the equipping of the
worktable.
B
Barcode reader
Device for scanning a barcode.
C
CleanCap
Optional epMotion equipment. The CleanCap includes a HEPA filter and at least one UV lamp.
Command
Step in a procedure. Various parameters are set for commands. Each command triggers an action, e.g., the
transport of liquids.
Control device
Device for controlling the epMotion. Depending on the equipment of the epMotion, a MultiCon PC or a
EasyCon Tablet is available.
CSV file
Text file for saving and exchanging structured information.
D
Destination labware
Rack or plate with destination positions.
Destination position
Position in the labware that exclusively is destination of liquid transfers.
Dispensing tool
Tool that aspirates and dispenses liquid. Single-channel dispensing tools and eight-channel dispensing
tools are available for various volume ranges.
E
Entry list
ID list assigned to a labware. The ID list contains the name of the labware, IDs for the positions of the
labware and descriptions. epBlue imports and exports the IDs of the labware with entry lists. epBlue can
process entry lists in the formats CSV and XML.
11
12
Operating instructions
epBlue™ with MultiCon
English (EN)
epBlue
Software for creating and administrating applications and labware. The epBlue software is used for
controlling the epMotion.
epBlue ID
With the epBlue ID module, you can track samples during the application. The labware is labeled with IDs
for this purpose. The sample tracking is documented automatically.
epT.I.P.S. Motion
Pipette tips of the epMotion. epT.I.P.S.® Motions are available with or without a filter. The abbreviation
stands for Eppendorf Totally Integrated Pipetting System.
F
Filling volume
Maximum volume of a labware. The filling volume is higher than the working volume. The epMotion
removes liquid from the labware up to the filling volume.
G
Gripper
Tool that transports labware.
H
Height adapter
Adapter for low labware. The height differences between the labware is offset to decrease the tool holder
paths, and therefore decrease the cycle time of the application.
HEPA filter
Air filter. The filter prevents particles such as dust and germs from the environment entering the epMotion
workspace.
I
ID
Character string which is used by the epBlue ID module to identify the labware.
Input list
ID list containing information on the source labware. The input list contains the IDs of the source positions.
epBlue creates an input list for each source labware with activated ID tracking.
Intermediate labware
Rack or plate with intermediate positions.
Intermediate position
Position in the labware that is source and destination of liquid transfers.
L
Lab administrator
User possessing the rights for the administration of applications and labware.
Operating instructions
epBlue™ with MultiCon
English (EN)
Labware
Racks, plates, tips, etc. which are placed on the worktable.
Location
Area where labware is placed on the worktable.
M
MagSep
System for nucleic acid preparation with the epMotion. The MagSep consists of a magnetic finger module,
an Eppendorf ThermoMixer module, a PrepRack, and reagents.
MultiCon PC
A computer with touchscreen. You can control your epMotion using the computer and the epBlue software.
P
Pattern
Pattern in which liquid is aspirated and dispensed. Patterns are defined in a transfer command. Patterns are
defined as regular patterns and irregular patterns. Patterns are independent in x-direction and y-direction.
Procedure
Sequence of commands executed consecutively. Part of an application.
R
Rack
Mount for tubes or pipette tips.
Remaining volume
Volume that cannot be removed from a vessel. Caused by the distance from the pipette tip to the vessel
base, defined in the software, and the defined immersion depth of the pipette tip in the liquid. The pipette
tip cannot aspirate the volume. The remaining volume depends on the vessel geometry.
Reservoir
Reservoirs are used for preparing reagents. Reservoirs are hung in a ReservoirRack or placed directly on
the worktable.
Result list
ID list with information on destination labware and intermediate labware. The result list contains the IDs of
destination positions and intermediate positions. epBlue creates a result list for each destination labware
and intermediate labware for which ID tracking is active.
S
SafeRack
Rack with ep.T.I.P.S. Motion pipette tips. The SafeRack features a partition that prevents the contamination
of adjacent tips. If you want to use the tips several times, use SafeRacks.
Source labware
Rack or plate with source positions.
Source position
Position in a labware that exclusively is a source of liquid transfers.
13
14
Operating instructions
epBlue™ with MultiCon
English (EN)
T
Thermal module
Heat plate for labware. The heat plate is integrated in the worktable.
Thermoadapter
Temperature-controlled adapter that is not transported with the gripper. Plates are inserted into the
thermoadapter.
Thermoblock
Temperature-controlled adapter that is transported with the gripper.
There are 2 types of thermoblocks:
• Thermoblocks: Thermoblocks, into which plates are inserted.
• Thermoblocks with plates Thermoblocks that are permanently connected with a plate.
Thermorack
Temperature-controlled rack for smaller vessels, e.g., Safe-Lock tubes for 0.5 mL, 1.5 mL or 2 mL.
Tube
A micro test tube or a single well in a plate.
V
Vacuum unit
The vacuum unit uses a vacuum to suck liquid from a filter plate. The vacuum unit consists of a vacuum
manifold, vacuum pump, and Vac Frame.
W
Work volume
Filling volume minus volume of the immerging pipette tip. The epMotion fills the vessels up to the working
volume.
Worktable
epMotion work surface that labware and tools are placed on. In the software, the epMotion worktable is
represented as epBlue worktable.
1.6
Version overview for the epBlue with MultiCon software manual
Order no.: 5075 900.866
Version
Issue date
Change
00
August 2013
• Creation. Software version 40.1.
01
December 2013
• Revision of the ID tracking function. Software version 40.1.
02
July 2015
• Update to software version 40.4.
Safety
epBlue™ with MultiCon
English (EN)
2
Safety
The intended use and safety instructions sections from the hardware manual apply for using the epMotion
with the epBlue 40.X software.
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16
Safety
epBlue™ with MultiCon
English (EN)
Product description
epBlue™ with MultiCon
English (EN)
3
3.1
Product description
Features
With the epBlue software you control the epMotion. epBlue consists of several modules.
Which modules are available depends on the features of your epMotion and the specification of the
software.
3.1.1
epBlue
Modules
Application Editor
Description
• Create applications.
• Manage applications.
Operating manual
(see epBlue Application Editor - Creating and
editing applications on p. 41)
(see epBlue Application Editor - Managing
applications and folders on p. 99)
Application Runner
• Execute applications.
(see epBlue Application Runner - Carrying out the
application on p. 25)
Application Simulator
• Simulate applications.
(see epBlue Application Simulator - simulating
applications on p. 123)
ID list management
• Manage ID lists.
(see epBlue ID - Working with ID tracking on
p. 125)
Labware Editor
• Manage labware.
(see epBlue Labware Editor - Creating and
managing labware on p. 105)
Log Viewer
• Display and print logs.
(see epBlue Log viewer - Managing application
logs on p. 39)
Settings
• Check components of the
device.
• Perform basic settings.
• User administration.
(see epBlue settings - Checking components and
making the basic settings on p. 133)
• Backup database.
• Perform basic settings for
ID tracking.
(see epBlue settings - Creating and managing
users on p. 143)
(see epBlue Settings - Saving data and creating
print templates on p. 153)
(see epBlue ID - Working with ID tracking on
p. 125)
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Product description
epBlue™ with MultiCon
English (EN)
3.1.2
Assistants
Assistants are programs for a specific application. Assistants are optionally available and described in
separate operating manuals. The following assistants are available for epBlue:
Prep Assistant
• Nucleic acid preparation with Eppendorf MagSep kits. After that, a PCR setup can be performed.
PCR Assistant
• Create PCR master mixes and dilution series for quantification standards, normalization of sample
concentrations, and setting up PCR reactions.
LH assistant
• Normalization of sample concentrations.
Operation
epBlue™ with MultiCon
English (EN)
4
4.1
Operation
Starting and exiting epBlue
4.1.1
Starting epBlue
Prerequisites
• The device does not show any sign of external damage.
 Switch on the epMotion using the mains/power switch.
 Switch on the MultiCon PC.
 To start epBlue, double-click the epBlue symbol on the desktop.
 Alternatively, open the Windows start menu and select Programs > Eppendorf > epBlue.
epBlue is started. The login screen appears.
4.1.2
Starting epBlue for the first time
Prerequisites
• epMotion and MultiCon PC were installed by a service technician.
• epBlue has been started (see p. 19).
• epBlue displays the login screen.
1. Log in as administrator (see Logging in as administrator in epBlue on p. 143).
2. Create user accounts (see Creating a user account on p. 146).
3. Log off as administrator.
4. Log into epBlue as a user (see Logging in as user on p. 19).
4.1.3
Logging in as user
Prerequisites
• epBlue has been started (see p. 19).
• epBlue displays the login screen.
• The administrator has created a user account for the user.
1. Enter the name of the user account and the password.
If you have forgotten your password, contact your administrator (see p. 148).
2. Press the Login button.
The epBlue start screen appears. The symbols that are displayed depend on your user rights and the
features of your epMotion.
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20
Operation
epBlue™ with MultiCon
English (EN)
Abb. 4-1: epBlue start screen
1
2
3
4
5
Fig. 4-1:
epBlue start screen
1 Eppendorf menu
Exit the program.
4 Status area
Status of the epMotion.
2 Menu bar
Contains functions that can be accessed in all
applications.
5 Working range
Contents and applications.
3 Information area
Contains information on the open file.
4.1.4
Logging off as a user
You can only log off if all applications started from your user account have been exited. If you want to log
off before completing an application, the application has to be ended manually.
Prerequisites
• All applications have been ended and saved.
• Changes to the labware have been saved.
 In the File menu, select the Log off entry.
You are logged out of your account. The login screen appears.
4.1.5
Ending epBlue and switching off the operating unit
1. In the Eppendorf menu, select Close.
2. When the program is ended, shut down MultiCon PC.
3. Switch epMotion off at the mains/power switch.
Operation
epBlue™ with MultiCon
English (EN)
4.2
Initial steps
4.2.1
Using touchscreen and mouse
You can operate the operating unit via the touchscreen or use a USB mouse.
 Connect the mouse to a USB interface on the operating unit.
Tab. 4-1:
Touchscreen/mouse comparison
Touchscreen
Mouse
Tap
Click using the left mouse button
Tap and hold 2 s
Click using the right mouse button
Drag
Drag with the left mouse button pressed down
Tap twice
Double-click using the left mouse button
4.2.2
Using the screen keyboard
Enter the numbers and letters using the screen keyboard.
Abb. 4-2: Screen keyboard
Fig. 4-2:
Screen keyboard
Automatically show the screen keyboard
 epBlue automatically shows a keyboard if you have selected an input field.
Manually show the screen keyboard
 In the File menu, select the Show keyboard entry.
Switch the automatic display of the screen keyboard on and off.
 In the File menu, select the Show keyboard automatically entry.
Tab. 4-2:
epBlue key combinations
Enter special characters.
 Press the letter key for 2 s.
Confirm your entry.
 Press the key once.
Switch between lowercase
letters and capital letters.
 To enter a capital letter, press the key once. Then press the
Switch the keyboard.
 To switch between the English and German keyboards,
letter.
 To switch the keyboard to all capital letters, press the key
twice.
press the key once.
Show the screen keyboard.  Press the key once.
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Operation
epBlue™ with MultiCon
English (EN)
4.2.3
File menu
Abb. 4-3: File drop-down menu
Fig. 4-3:
File drop-down menu
Exit to start screen entry
Show the epBlue start screen.
Sound enabled entry
Switches the system sound on and off.
Menu – Show keyboard
Manually show the screen keyboard.
Log Off entry
The user is logged off. The login window appears.
Menu – Show keyboard automatically
Switch the automatic display of the screen
keyboard on and off.
Abb. 4-4: Help drop-down menu
Fig. 4-4:
Help drop-down menu
Device manuals entry
Open the operating manual of the device.
Software manuals entry
Open the operating manual of the software.
About epBlue entry
Show the epBlue version.
Operation
epBlue™ with MultiCon
English (EN)
4.2.4
Status area
The status area is located in the top right of the screen.
If you click the epMotion in the status area, the following information is displayed:
• epMotion serial number.
• Equipment of the epMotion
• Firmware version
• Status of the epMotion
• Actual temperature of a thermal module or an Eppendorf ThermoMixer module.
Example Info: C3:21°C. The thermal module in location C3 has a temperature of 21°C.
4.2.5
Enlarging the view
You can enlarge the image of a set of labware. All positions of the labware become clearly visible. The
enlarging function is a help, e.g. for patterning. You can use the navigation window for better navigation in
the enlarged image.
Abb. 4-5: View window
Fig. 4-5:
View window
Decrease the image in the view window.
Press button or scroll with the mouse wheel.
Enlarge the image in the view window.
Press button or scroll with the mouse wheel.
72 %
Display the image in the view window at the desired percentage.
Show the navigation window.
Press the Cross button and move the navigation window.
Alternatively, press the Ctrl key on the keyboard. Press and hold the left mouse
wheel. Move the navigation window.
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24
Operation
epBlue™ with MultiCon
English (EN)
Abb. 4-6: Navigation window
Fig. 4-6:
Navigation window
Return to previous view.
Go to next view.
Scale image to 100%.
Scale image to the size of the view window.
Center image in the view window. The enlargement remains
unchanged.
Close the navigation window.
epBlue Application Runner - Carrying out the application
epBlue™ with MultiCon
English (EN)
5
epBlue Application Runner - Carrying out the application
This chapter describes how to carry out an application on the epMotion.
5.1
Opening an application
Abb. 5-1: Selecting an application
Fig. 5-1:
Selecting an application
Prerequisites
• epMotion is ready for operation.
• Application Runner is started.
1. Select the application in the Select application area.
Recently used applications can be found in the Recently used area.
Open applications in the Select application area have a red padlock .
The Properties area displays the properties of the selected application.
2. Press the Next button.
The application is opened.
5.2
Positioning the labware on the epMotion worktable
 Position the labware on the epMotion worktable according to the application.
Additional information on positioning the labware can be found in the hardware operating manual.
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epBlue Application Runner - Carrying out the application
epBlue™ with MultiCon
English (EN)
5.3
Preparing the application run
5.3.1
Selecting the device
Abb. 5-2: Selection of an available device
Fig. 5-2:
Selection of an available device
NOTICE! Tool collisions with incorrectly positioned labware.
Labware must be positioned on the epMotion worktable as defined in the application.
Tools and labware become damaged in a collision. A collision can lead to sample loss.
 Check the positioning of the labware before starting the application.
In the Available Devices area, the connected epMotion is displayed.
Prerequisites
• epMotion is ready for operation.
1. Select the device for the application run.
2. Press the Next button.
epBlue Application Runner - Carrying out the application
epBlue™ with MultiCon
English (EN)
5.3.2
Entering the number of samples
Abb. 5-3: Process control window
Fig. 5-3:
Process control window
The window Process control appears if the number of samples in the application is variable.
1. Enter the number of samples for the current run.
2. Press the OK button.
5.3.3
Setting the HEPA air filter and the UV lamp
Abb. 5-4: Setting the parameters for the HEPA air filter and the UV lamp
Fig. 5-4:
Setting the parameters for the HEPA air filter and the UV lamp
If your epMotion is equipped with a HEPA air filter and UV lamp, you can define the settings for the HEPA
air filter and the UV lamp.
 Activate or deactivate the HEPA air filter.
 Define whether a decontamination is to be carried out before the application.
The worktable may only be decontaminated if no labware or tools are present on the worktable.
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epBlue Application Runner - Carrying out the application
epBlue™ with MultiCon
English (EN)
5.3.4
Positioning the labware on the epMotion worktable
 Position the labware on the epMotion worktable according to the application.
Information on positioning the labware can be found in the hardware operating manual.
5.3.5
Setting the run parameters for the optical sensor
If you have the epBlue ID module, the Use barcodes checkbox appears in the window.
In the application, you defined the application parameters for the optical sensor. You can enter run
parameters to use other settings of the optical sensor for an individual run. The run parameters overwrite
the application parameters for this run only (see Optical sensor on p. 93).
Abb. 5-5: Setting the run parameters for the optical sensor
Fig. 5-5:
Setting the run parameters for the optical sensor
Use Barcodes slider
Activate ID tracking.
Input volumes manually radio button
Enter the filling volume manually.
Detect volumes radio button
The optical sensor measures the filling volume.
In which positions the optical sensor measures
the filling volume is defined in the labware
properties.
Detect tips slider
The optical sensor checks in which positions
pipette tips are located in the labware.
Use required minimum volume radio button
epBlue assumes that all vessels are filled with the
minimum required volume for this run.
Check labware placement slider
The optical sensor checks if labware is on the
worktable.
epBlue Application Runner - Carrying out the application
epBlue™ with MultiCon
English (EN)
1. Configure the functions of the optical sensor.
2. Activate ID tracking, if necessary.
3. Press the Run button.
5.3.6
Setting the run parameters for the labware
You have defined in the labware properties how the optical sensor measures the filling level in the
application. In the run parameters, you can enter a different option for measuring the filling level. The run
parameters overwrite the application parameters for this run only (see Optical sensor on p. 93).
Abb. 5-6: Setting the run parameters for the labware
Fig. 5-6:
Setting the run parameters for the labware
Prerequisites
In the Volume settings, the Detect volumes is activated.
1. Select the labware.
The window with the labware properties appears.
2. For plates, select the mode in which the optical sensor measures the plate filling level. To do so, activate
the Random positions checkbox or the All used positions checkbox.
3. For ReservoirRack modules, highlight the ReservoirRack module in which the optical sensor measures
the filling level. In the table, activate the Level sensor checkbox.
The manual entry field is grayed out. You can no longer enter the volume manually. The optical sensor
measures the volume.
4. To close the labware properties window, press the Next button.
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epBlue Application Runner - Carrying out the application
epBlue™ with MultiCon
English (EN)
5.3.7
Setting the ID tracking parameters
If you have the epBlue ID module and ID tracking is activated for the labware, a window appears.
5.3.7.1 Entering IDs manually
Abb. 5-7: Entering IDs manually
Fig. 5-7:
Entering IDs manually
Prerequisites
• Labware ID tracking is activated.
1. Press the Next button.
You are prompted to enter the IDs for the labware. The labware is marked with a red border.
2. Enter the names for the ID list that is created during the application for this labware in the List ID field.
3. Read the ID with the barcode reader or enter it manually.
4. If 2 IDs are assigned for a reagent, 2 entry fields appear. If you are using only one ID, press the
Use only one ID button.
5. Confirm your entry with the OK button.
6. Press the Next button to enter the ID for the next labware.
7. When all IDs have been entered, the application starts.
epBlue Application Runner - Carrying out the application
epBlue™ with MultiCon
English (EN)
5.3.7.2 Reading the IDs with an entry list
Abb. 5-8: Select entry list
Fig. 5-8:
Select entry list
Prerequisites
• Labware ID tracking is activated.
1. Press the Next button.
You are prompted to enter the IDs for the labware. The labware is marked with a border.
2. Press the Select ID list button.
The Select ID list window appears.
By default, the window shows all compatible entry lists.
3. Import the entry list, if required (see p. 129)
4. Select the entry list to be imported.
5. Press the OK button.
6. Enter the ID for the selected position.
epBlue compares the entered ID with the ID of the entry list.
If the ID does not correspond to the ID of the entry list, an error message appears.
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epBlue Application Runner - Carrying out the application
epBlue™ with MultiCon
English (EN)
7. Correct the wrong ID.
If the entry list contains more positions than required, a message appears.
8. If you continue the import, only the required IDs will be imported.
9. To select a different entry list, cancel the import.
10. When all IDs have been entered, the application starts.
Abb. 5-9: Imported entry list
Fig. 5-9:
Imported entry list
epBlue Application Runner - Carrying out the application
epBlue™ with MultiCon
English (EN)
5.3.8
Entering the filling volume of the labware
5.3.8.1 Measuring the filling volume with the optical sensor
If the Detect volumes function is active, the optical sensor checks the filling volume after the start.
If the following problems occur, a dialog window appears:
• Filling volume too low.
• Filling volume too high.
• Filling volume cannot be correctly detected.
Abb. 5-10: Checking window
Fig. 5-10: Checking window
Maximum volume
Maximum filling volume for the position.
Abort button
Aborts the application.
Minimum volume
Filling volume required to remove the set amount of
fluid.
User input button
Entering the filling volume manually
Calculated volume
Calculated filling volume for the position. The filling
volume is calculated from the labware data and the
fluid level.
Accept level and continue button
Accept the calculated filling volume.
µL input field
If the User Input button has been selected,
enter the filling volume manually.
Repeat scan button
Measuring the filling volume again with the optical
sensor
NOTICE! Dispensing error due to refilling of vessels.
The optical sensor only checks the level of the vessels at the specified location.
 Only change the volume of the vessels at the specified location.
 Do not change the volume of the vessels at other locations.
 If the Calculated volume is larger than the Maximum volume, remove liquid from the vessel.
 If the Calculated volume is smaller than the Minimal volume, add liquid to the vessel.
 If there is an air bubble in the liquid, the optical sensor cannot perform a measurement. Remove the air
bubble. To repeat the detection, select the Repeat scan button.
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epBlue Application Runner - Carrying out the application
epBlue™ with MultiCon
English (EN)
5.3.8.2 Entering the filling volume manually
If the Input volumes manually function is active, you have to enter the filling volume manually after the start.
Abb. 5-11: Entering the filling volume
Fig. 5-11: Entering the filling volume
Labware info column
Information on the selected labware.
Worktable column
epBlue worktable with selected labware
Center column
Entering the filling volume, see table
Tab. 5-1:
Center table
Column
Meaning
Red border
Invalid filling volume entered. Filling volumes that are smaller than the required
volume are invalid. Filling volumes that are greater than the maximum volume of the
labware are invalid.
Index column
Position in the labware.
Name checkbox
Configure the settings of the optical sensor.
MIN column
Minimum filling volume that is required for this run.
VOL [UL] column
Enter the filling volume manually.
MAX column
Maximum filling volume the labware can be filled with.
epBlue Application Runner - Carrying out the application
epBlue™ with MultiCon
English (EN)
Proceed as follows:
 Enter the filling volume in the VOL [UL] column.
 To enter the same filling volume for the entire labware, press the Set all volumes button. Enter the filling
volume.
 Enter the run parameters of the optical sensor in the Liquid detection area, if necessary.
 To define that epBlue shall use the minimum filling volume for a position, press the Use minimal volume
button.
 Press the Run button.
5.4
Starting the application
Prerequisites
• Labware is positioned on the epMotion worktable.
• epMotion is ready for operation.
• The application run was prepared (see p. 26).
 Click on the Run button.
The application starts.
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epBlue Application Runner - Carrying out the application
epBlue™ with MultiCon
English (EN)
5.5
Controlling the application
Abb. 5-12: Ongoing application
Fig. 5-12: Ongoing application
Carry out the application. The epMotion executes
the application until the end.
Executing the application step-by-step. The
epMotion executes the next step and then stops.
Pause. The application stops, but it will not be
aborted.
Procedure area
Commands. The active command is highlighted
blue.
Worktable area
Worktable.
Remaining Runtime area
Status of the application/runtime of the
application/total time for the application.
Log area
Log.
Aborts the application. If you have stopped the
application using the button, the button is
now active.
 Control the application with the buttons from the Control area.
epBlue Application Runner - Carrying out the application
epBlue™ with MultiCon
English (EN)
5.6
Ending the application
When the application run is finished, a message appears.
Abb. 5-13: The application is finished
Abb. 5-14: The application was canceled
Fig. 5-13: The application is finished
Fig. 5-14: The application was canceled
1. To confirm the message press the OK button.
2. In the Application Runner window, press the Next button.
The final window appears. If your epMotion is equipped with a HEPA air filter and UV lamp, you can
activate the HEPA air filter and the UV lamp.
Abb. 5-15: Final window
Fig. 5-15: Final window
3. Activate the HEPA air filter, if necessary.
Prerequisites
• The worktable of the device must be cleared completely before activating the UV lamp.
4. Activate the UV lamp, if necessary.
5. Press the Exit button to end the application.
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epBlue Application Runner - Carrying out the application
epBlue™ with MultiCon
English (EN)
epBlue Log viewer - Managing application logs
epBlue™ with MultiCon
English (EN)
6
epBlue Log viewer - Managing application logs
The chapter also describes how to view and print application logs.
6.1
Displaying protocols
Abb. 6-1: Logs window
Fig. 6-1:
Logs window
Prerequisites
• The application was executed (see p. 35).
• The epBlue start screen is shown.
1. Click on the LogViewer symbol.
Select the folder in which the application is saved.
2. Select the log.
The log is opened.
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epBlue Log viewer - Managing application logs
epBlue™ with MultiCon
English (EN)
6.2
Saving the log as a PDF file
Prerequisites
1. Click on the
symbol.
The print preview opens.
2. Check the layout and set up the page.
3. Save or print the document as a PDF file.
4. Close the window.
Tab. 6-1:
Symbols in the print preview
Search in the document.
Show the first page.
Print document.
Show the previous page.
Page setup.
Show the next page.
Scale the document.
Show the last page.
Zoom in the view.
Create a PDF.
Set the zoom factor.
Close the view.
Zoom out the view.
epBlue Application Editor - Creating and editing applications
epBlue™ with MultiCon
English (EN)
7
epBlue Application Editor - Creating and editing applications
This chapter describes how to create and edit applications in epBlue.
7.1
Overview
Applications are programmed processes in epBlue. An application consists of equipping the worktable and
a sequence of commands. Each command triggers a specific action.
Applications are created in a fixed order:
1. Create an empty application (see p. 42).
2. Position labware and define the application parameters of the labware (see p. 43).
3. Activate ID tracking of the labware (see p. 47).
4. Define the procedure (see p. 49).
5. Check the application (see p. 52).
6. Simulate the application (see p. 123).
7.2
Creating an application
7.2.1
Menu bar
Abb. 7-1: Application Editor menu bar
Fig. 7-1:
Application Editor menu bar
Saving the application.
One step back.
Saving the application for another worktable.
One step forward.
CSV
Saving all applications.
Import CSV file.
Closing the application.
Printing the application.
Cutting the application.
Checking the application.
Copying the application.
Go to simulation.
Inserting the application.
Go to Application Runner.
Deleting the application.
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epBlue Application Editor - Creating and editing applications
epBlue™ with MultiCon
English (EN)
7.2.2
Creating an empty application
Abb. 7-2: Start screen of the Application Editor
Fig. 7-2:
Start screen of the Application Editor
Prerequisites
• The Application Editor is open.
1. Select the folder in which the application is saved.
2. Click on the
symbol in the menu bar.
The Create new application window appears. epBlue detects the connected device.
3. Enter the name and description of the application.
4. Close the window using the Create button.
You have created an empty application. The Worktable window of the application opens.
7.2.3
Copying applications
You can create new applications by copying and changing existing applications.
The Eppendorf folder contains applications defined by Eppendorf AG. These applications
cannot be executed or changed. They are used as templates and examples.
 In order to use applications from the Eppendorf folder, copy the corresponding application
to your folder.
 To copy an application (see p. 100).
7.2.4
Saving applications
 To save an application, click on the
symbol in the toolbar.
 To save an application under a new name, click on the
 To save all open applications, click on the
symbol in the toolbar.
symbol in the menu bar.
epBlue Application Editor - Creating and editing applications
epBlue™ with MultiCon
English (EN)
7.2.5
Positioning labware
7.2.5.1 Positioning the labware on the epBlue worktable
Labware can only be positioned at specific locations. Information on positioning the labware can be found
in the hardware operating manual.
Abb. 7-3: Positioning the labware on the epBlue worktable
Fig. 7-3:
Positioning the labware on the epBlue worktable
Labware Category column
Overview of all labware types.
Worktable area
epBlue worktable with positioned labware.
Labware area
Labware that can be positioned on the epBlue
worktable.
Properties area
Properties of the selected labware.
Switch to procedure button
To go to the Procedure window, press the Switch Placed Labware column
to procedure button.
Labware positioned on the epBlue worktable.
Trash
Delete the selected labware from the epBlue
worktable.
Prerequisites
• Labware combinations have been created for reaction vessels (see p. 106).
• The application is open.
• The Worktable window is open.
1. Select labware from the Labware area.
2. Draw the labware to the desired position of the epBlue worktable.
If the labware cannot be positioned at this location, the location is marked in red.
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epBlue Application Editor - Creating and editing applications
epBlue™ with MultiCon
English (EN)
7.2.5.2 Deleting positioned labware from the epBlue worktable
There are several options to delete labware from the epBlue worktable.
Selecting the labware
 Activate the checkbox for the corresponding labware in the Placed labware column.
 Alternatively, select the labware with the mouse.
Deleting labware
 Drag the selected labware into the trash with the mouse.
 Alternatively, click on the
symbol in the menu bar.
 Alternatively, select the Delete function in the context menu.
7.2.5.3 Stacking labware
The gripper stacks and transports labware. The gripper is required to stack labware or to unstack a stack
during an ongoing application.
You can stack a maximum of 5 plates on the worktable. You can stack a plate on a height adapter. The
maximum stacking height is 126 mm. Stacked labware must have the same properties, e.g. name,
geometry, and bottom tolerance.
The gripper only transports one part at a time. The gripper can stack labware or unstack a stack of labware.
Liquids cannot be transferred in a plate stack.
Functions of the optical sensor with stacked plates
• The Check labware placement function is available.
• The Detect volumes function is not available.
You can stack the following plates:
• EP_pDNA_96_MTP
• EP_TT_PCR_150
• EP_TT_PCR_40
• EP_DWP_1200
• EP_pDNA_96_DWP
• EP_DWP_200
• EP_DWP_2000
• EP_DWP_1000
• EP_DWP_500
• EP_MTP_384
• EP_MTP_96
• EP_MTP_UV_VIS_96
• EP_MTP_CC_96
• EP_MTP_CI_96
• EP_MTP_CC_6
• EP_MTP_CC_12
epBlue Application Editor - Creating and editing applications
epBlue™ with MultiCon
English (EN)
•
•
•
•
•
EP_MTP_CC_24
EP_MTP_CC_48
EP_TT_PCR_150
EP_TT_PCR_250
EP_TT_PCR_40
The list is extended if new labware is available.
On the vacuum unit, you can stack 4 labware components. Information on the vacuum unit can be found in
the hardware operating manual.
Plates that are stacked may only be filled up to the working volume.
Abb. 7-4: Stacking labware on the epBlue worktable
Fig. 7-4:
Stacking labware on the epBlue worktable
Proceed as follows to stack labware at a location.
1. Place the lower labware at one location.
2. Position the upper labware at the same location.
The Placed labware column shows, which labware is placed in the epBlue worktable locations.
The number behind the designation shows how many parts have been stacked at this location. Example:
pcr384_3. Only 3 plates have been stacked.
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7.2.6
Defining the application parameters of the labware
You can determine the properties of each labware used.
Abb. 7-5: Properties window
Fig. 7-5:
Properties window
 To change the properties of a labware, double-click on the labware.
 Alternatively, select the labware and go to the Properties context menu with the right mouse button or
click on the Properties button.
The Properties window opens.
 Set the properties for the selected labware.
7.2.6.1 Defining the filling volume of the labware
1. Set how the filling volume of the labware is to be measured.
2. To measure the filling volume with the optical sensor (see Optical sensor on p. 93)
3. To enter the filling volume manually (see Entering the filling volume manually on p. 34)
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7.2.6.2 Activating ID tracking of the labware
In order for epBlue to create an ID list for a labware, ID tracking of the labware must be active.
ID tracking is activated separately for each labware. To track IDs, activate ID tracking for source labware,
intermediate labware, and destination labware.
Prerequisites
• The application is open.
• The Worktable window is open.
 Set whether ID tracking is to be carried out for the labware.
The following options are available for ID tracking at the start of the application:
• Manual scan: Enter the ID manually or read it with the barcode reader.
• Entry list: Select ID list. To check the ID list, enter the first position of the list or the list ID when starting
the application.
• No: The labware is not scanned.
7.2.6.3 Opening sealed labware
The epMotion can open the sealing of individual positions of a labware. The epMotion opens the sealing
with a single-channel dispensing tool.
If the Pierce options option is activated, epBlue can open the sealing of the labware with the following
commands:
• Commands Sample transfer, Reagent transfer, Dilute, Pool, Pool one destination: The sealing is opened.
Immediately afterwards, a liquid transfer is performed.
• Pierce command: The sealing is opened. Immediately afterwards, a liquid transfer is performed.
To pierce the sealing there are 2 modes.
• Single pierce mode: The pipette tip on the dispensing tool pierces the aluminum foil once in the middle
of the position.
• Triple pierce mode: The pipette tip on the dispensing tool pierces the aluminum foil three times. The
holes have a fixed distance to the middle of the position. The holes are pierced into the aluminum foil at
a distance of 120°. The Triple pierce mode is used to pierce a big hole into the sealing with small
dispensing tools.
The single-channel dispensing tool TS 1000 opens the sealing in factory setting in the Single pierce mode.
The single-channel dispensing tools TS 50 and TS 300 open the sealing in factory setting in the Triple
pierce mode.
You can change the mode in which the dispensing tool opens the sealing. For this purpose, change the
value in the Distance (see Fig. 7-6 on p. 48) field.
Example: The single-channel dispensing tool TS 1000 opens the sealing in factory setting in the Single
pierce mode. The distance of the dispensing tool to the middle of the position is zero. The value in the
Distance field is zero. You change the value in the Distance field to 1. The single-channel dispensing tool
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TS 1000 will open the sealing in the Triple pierce mode. The holes have a distance of 1 mm to the middle of
the position.
After the sealing has been opened, the pipette tips can still be used or ejected (see Pierce command on
p. 69).
If the labware is sealed, the optical sensor cannot measure the filling level. If the Pierce settings option is
activated, the Levelsensor settings option is not available.
The labware must be sealed with aluminum foil that is marked with the word "Pierceable".
If you have any questions on the suitable aluminum foil, please contact Automations Support at Eppendorf.
Write an e-mail to [email protected]
The parameters in the Pierce settings area have been optimized for the labware combination Thermoblock
PCR 96 OC with twin.tec PCR Plate 96, semiskirted.
Setting the parameters for opening sealed labware
Abb. 7-6: Properties window in the Pierce settings area
Fig. 7-6:
Properties window in the Pierce settings area
Distance
Above
Distance from the middle of the vessel.
Distance between the starting position
If the distance is zero, the single-channel dispensing tool
of the piercing movement and the vessel
opens the sealing in the Single pierce mode.
edge. At this distance, the pipette tip
If the distance is > zero, the single-channel dispensing tool
starts piercing.
opens the sealing in the Triple pierce mode.
Speed
Depth
Speed at which the pipette tip opens the
Distance between vessel edge and pipette tip. This is how
sealed labware.
far the pipette tip is immersed into the vessel.
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1. Select the labware.
2. Call up the properties of the labware.
The Properties window opens.
3. Set parameters in the Pierce settings area.
4. Place the labware with an adapter on the worktable.
5. Perform a test run. Check whether the parameters in the Pierce settings area are adjusted to labware and
aluminum foil.
7.2.6.4 Setting the filling level of the labware
You can set the filling level for all positions of the labware.
1. To set the same filling level for all positions of a labware, press the Set all volumes button.
2. To import the filling levels of the labware from a CSV file, press the Import all volumes button.
3. To enter the filling levels of the labware manually, enter the filling levels in the VOL [µL] column.
7.2.7
Defining procedures
A procedure is a defined sequence of commands which epMotion executes during an application.
Commands are described in the Commands chapter (see p. 53).
The first command of each procedure is the Procedure Start command. The Procedure Start command is
created automatically and cannot be deleted. The Procedure Start command shows the epBlue worktable at
the start of the application. The Procedure Start command does not influence the application.
The software does not include any command for taking up pipette tips. The user defines which pipette tips
will be used in a command. The user must provide for the corresponding pipette tips on the worktable.
The user defines the dispensing tool for each command. Tools are picked up according to the command
parameters.
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Abb. 7-7: Creating a procedure for an application
Fig. 7-7:
Creating a procedure for an application
Commands column
Available commands.
Procedure column
Sequence of commands. The commands are
executed in the order displayed.
Prerequisites
• The application is open.
• Labware is positioned on the epBlue worktable .
• The Procedure window is open.
Worktable
The state of the worktable during the execution of
the selected command
Options area
Parameter of the selected command.
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7.2.7.1 Inserting a command
1. Select a command from the Commands column.
2. Drag the command to the Procedure column.
3. In order to insert a command at the end of the application, double-click on the selected command in the
column Commands.
7.2.7.2 Copying commands
 Select a command from the Procedure column.
 Click on the
symbol in the menu bar.
 Alternatively, open the context menu with the right mouse button and select Copy.
7.2.7.3 Inserting a copied command
1. Copy the command.
2. Select the position in the Procedure column where you would like to insert the copied command.
3. Select the Paste command in the context menu.
The copied command is inserted behind the selected position.
7.2.7.4 Changing the command sequence
 Select a command in the Procedure column.
 Drag the command to the desired position in the procedure.
7.2.7.5 Deleting commands
 Select a command in the Procedure column.
 Delete the command using the symbol in the menu bar or via the context menu.
7.2.7.6 Changing command parameters
1. Select a command in the Procedure column.
The parameters of the selected command are shown in the Options area.
2. Set the parameters to suit your requirements.
7.2.7.7 Inserting commands from another application
You can insert a command, several commands, or a procedure from another application.
Prerequisites
• Source application and destination application have been opened in the epBlue Application Editor.
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1. Copy the command in the source application (see p. 51)
2. Insert the command in the destination application (see p. 51)
Abb. 7-8: Message on adapting the worktable
Fig. 7-8:
Message on adapting the worktable
When a command is inserted, a message appears.
It can be defined for each user whether the message appears (see Activating warning messages on
p. 152)
3. Check the parameters of the copied command and adapt them, if necessary.
4. Check whether the worktable corresponds to the changed commands.
7.2.7.8 Inserting labware from another application
You can insert labware from an epBlue worktable of another application.
Prerequisites
• Source application and destination application have been opened in the epBlue Application Editor.
1. Select the labware on the epBlue worktable of the source application.
2. To copy the labware, click on the
symbol in the menu bar.
3. Alternatively, select the Paste command in the context menu.
4. Select the position for the labware on the epBlue worktable of the destination application.
5. To insert the labware, click on the
symbol in the menu bar.
6. Alternatively, select the Paste command in the context menu.
If a labware with the same name exists in the destination application, a message appears.
7. Change the name of the labware.
8. Check whether there are commands that use the former name of the labware.
9. Adapt the commands.
7.2.8
Checking an application
1. Click on the
symbol in the menu bar.
2. Select the Check command function.
The application is checked.
Example: Pipette tips without filter are positioned on the epMotion worktable. In the application, pipette
tips with filter were selected. The screen displays an error message.
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7.3
Commands
A procedure is a sequence of commands that epMotion executes during an application run. Commands are
executed one after the other and independent of each other. Each command has a specific set of
parameters.
The software does not include any command for taking up pipette tips. The user defines which pipette tips
will be used in a command. The user positions the corresponding pipette tips on the worktable.
The user defines the dispensing tool for each command. Tools are picked up according to the command
parameters.
The command parameters may differ. The displayed parameters depend on the selected
worktable or on the configuration of the connected epMotion.
Transfer commands
Sample transfer
Reagent transfer
Dilute
Pool
Pool one destination
Additional commands
Mix
ThermoMixer
epMotion with
Eppendorf
ThermoMixer
module
Magnetic separation Temperature
epMotion with
epMotion with
magnetic finger
thermal module
module
Transport
epMotion 5075
epMotion 5073
Exchange
epMotion 5070
Wait
User intervention
Number of samples
BAR
External process
Pierce
Vacuum
epMotion with
vacuum unit
Comment
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7.3.1
Sample transfer command
Various transfer commands are available to transfer liquids. All transfer commands are derived from the
Sample transfer command.
Abb. 7-9: Principle of the Sample transfer command
Fig. 7-9:
Principle of the Sample transfer command
The Sample transfer command is used to transfer liquids from various source positions to several
destination positions. The number of samples that will be aspirated from the source labware depends on
the Number of samples (see p. 74) command.
If the Pierce settings function is activated in the properties of the labware, the labware will be opened before
the liquid transfer. If the sealing of a source vessel and the sealing of a destination vessel are to be opened,
the source vessel is opened first. To open the vessels, epBlue uses the same dispensing tool and the same
pipette tips as for liquid transfer.
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7.3.1.1 Sample transfer > Parameter command
Abb. 7-10: Sample transfer > Parameter
Fig. 7-10: Sample transfer > Parameter
Volume
Select volume in μL or nL. A decimal place is available for volumes ≤ 99.9 μL.
You can transfer a volume larger than the maximum volume of the pipette tip.
The volume to be transferred will be pipetted in several steps.
Example: The user would like to transfer 1500 μL and enters this value into the
Volume field. If a 1000 μL tip is being used, 2x 750 μL will be pipetted. If a
300 μL tip is being used, 5 x 300 μL will be pipetted.
Pipet. tool
Used dispensing tool.
Pipette
The volume is individually aspirated and dispensed for each liquid transfer.
Multidispense
Aspirate liquid and dispense it in the destination position in defined subsets.
Multiaspirate
Aspirate liquid from several source positions using one pipette tip and
dispense the liquid in one destination position. Available for Pool and Pool one
destination commands .
Use filter tips
Activate when using tips with filter.
Source
Select the source labware. The source labware must be placed on the
worktable. When using more than one source labware, the number of positions
in the labware (384, 96, 24) must match. If you dispense within one labware,
the source labware and destination labware are identical.
Destination
Select the destination labware. The destination must be placed on the
worktable. When using more than one destination labware, the number of
positions in the labware (384, 96, 24) must match. If you dispense within one
labware, the source labware and destination labware are identical.
Pattern
Select the source positions and destination positions. Create replicates.
Information on the aspiration and transfer of liquids with the epMotion can be found in the
hardware operating manual.
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7.3.1.2 Sample transfer > Options command
Abb. 7-11: Sample transfer > Options
Fig. 7-11: Sample transfer > Options
Aspirate from bottom
Aspirate liquid from the base of the vessel. When using several vessels with
different volumes, enter the lowest filling volume of these vessels (Sample
transfer > Parameter).
Elution from filter
Aspirate liquids from the supernatant in filter plates. Available if in the
Parameter window the Pipette radio button is selected.
Dispense from top
During liquid dispensing, position the pipette tips 3 mm – 4 mm below the
edge of the vessel. When using several vessels with different volumes, enter
the highest filling volume of these vessels (Sample transfer > Parameter).
Change Tips
Defined time when the pipette tips are changed.
when command finished
Eject pipette tips after the end of the command. The liquid will be aspirated
with the same tip from all source labware positions.
before asp. for next
destination well
Eject the pipette tips before the liquid from the next source position is
aspirated. Liquid from the same source position will be aspirated with the same
tip. Liquid from the next source position will be aspirated using a new tip.
before each aspiration
Eject pipette tips prior to each aspiration. Liquid will always be aspirated with
a new tip. Additional volume will be discarded in the waste container along
with the pipette tip.
Dip tips
After liquid dispensing, a drop may remain on the pipette tip. The drop is
stripped off at the surface of the liquid.
Adjust, how close the pipette tip may approach the surface of the liquid after
liquid dispensing.
The surface of the liquid has the value zero. Positive values indicate the
distance from the surface of the liquid into the liquid. Negative values indicate
the distance from the surface of the liquid to the top.
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keep tips, do not change
tips
Do not eject pipette tips. Liquid will be aspirated with the same tip from all
source positions. The tips are also used in the next command. If the transfer
type changes in one of the following commands, or the Mix command is used,
the tips will be ejected.
after XX aspirations
Pipette tips will be ejected after a defined number of aspirations.
Restore tips
Pipette tips are restored to the position of the box they were taken from.
Is only executed if the tips within an application are reused for the same source
vessel.
The restore tips checkbox is only available with specific settings of the Change
Tip option.
Reuse tips
Restored pipette tips will be reused.
The pipette tips will be reused within an application for the same source vessel.
Is only executed if the restore tips option was selected in a previous command
and the restored tips are available.
The re-use tips checkbox is only available with specific settings of the Change
Tip option.
Information on the aspiration and transfer of liquids can be found in the hardware operating
manual.
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7.3.1.3 Sample transfer > Mix command
The Mix command is also available for mixing (see p. 73).
Abb. 7-12: Sample transfer > Mix
Fig. 7-12: Sample transfer > Mix
Mix before aspirating
Mix before each aspiration. Set the parameters following activation.
If you transfer a larger volume than the maximum volume of the pipette, the
liquid will be mixed before the first aspiration.
Mix after dispensing
Mix after each dispensing. Set the parameters following activation. Available
for Transfer type > Pipette.
If you transfer a larger volume than the maximum volume of the pipette, the
liquid will be mixed after the last aspiration.
No. of Cycles
Number of mixing cycles.
Speed
Mixing frequency. If no entries were made for Speed, the defined aspiration
speed of the liquid type will be used.
Volume
Mixing volume.
Fixed height
Position of the pipette tip on the Z-axis. Fixed aspiration level and dispensing
level. Only for levels below the working volume.
Aspiration
Distance between the pipette tip and base of the vessel during aspiration. If
0 mm is entered, the distance will be corrected to 2 mm. The correction is
dependent on the bottom tolerance and type of labware.
Dispense
Distance between the pipette tip and base of the vessel during dispensing. If
the dispensing position is above the vessel, the distance will be corrected to
the height of the vessel.
Information on mixing liquids can be found in the hardware operating manual.
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7.3.1.4 Sample transfer > Rinse options
Abb. 7-13: Sample transfer > Rinse options
Fig. 7-13: Sample transfer > Rinse options
You can clean used pipette tips using the Sample transfer > Rinse options command. The pipette tips are
rinsed in washing solution after the liquid transfer.
If the re-use tips checkbox is activated in the Sample transfer > Options window, the pipette tips will be
placed on the rack they were taken from after being cleaned. If the checkbox is not activated, the pipette
tips are disposed of after cleaning. You can set the parameters for this with the Sample transfer > Options
command.
Rinse tips before disposal Clean pipette tips.
checkbox
No. of Cycles
Number of cleaning cycles.
Speed
Cleaning speed. If no Speed value is entered, epBlue uses the aspiration speed
of the liquid type.
Volume
Volume of the washing solution that is aspirated into the pipette tip.
Liquid type
Liquid type of the washing solution.
Table Rinse racks
Vessel for washing solution.
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7.3.1.5 Sample transfer > Liquid types
Liquid types are defined in epBlue.
Information on dispensing liquids can be found in the hardware operating manual.
Tab. 7-1:
Defined liquid types in epBlue
Liquid type:
Optimized for
50 µL tips:
Pipetting
from
50 µL tips:
Dispensing
from
Comments
Alcohol 75%
Mixture of
75 % ethanol
and 25 % water
1 to 3 μL
3 μL
• Wash reagent in kits for nucleic
acid purification. See applications.
Alcohol 98%
Alcohol 98 %
1 μL
3 μL
• New pipette tips are pre-wetted
with the liquid to be aspirated.
Prewetting option is set (see p. 62).
• If you are using pipette tips with
300 μL in Multidispense mode and
aspirate a volume of 300 μL, the
gap to the filter is very small. The
filter may be wetted. For 300 μL
pipette tips and an aspiration
volume > 280 μL, use the Pipette
dispensing mode.
Glycerol
Mixture of
40 % glycerol
and 60 % water
1 μL
5 μL
• Liquid type for enzyme solutions.
• If the glycerol content of the
enzyme solutions is significantly
lower, liquid type Water can be
used.
Protein
Water with
1 % albumin
(10 g/L),
0.01 % Triton
X-100
5 μL
5 μL
• New pipette tips are pre-wetted
with the liquid to be aspirated.
Prewetting option is set (see p. 62).
• The bending of the surface of the
liquid has a negative impact on the
free jet capability of dispensing in
cell culture plates.
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Liquid type:
Optimized for
50 µL tips:
Pipetting
from
50 µL tips:
Dispensing
from
Comments
ProteinC
Water with
1 % albumin
(10 g/L),
0.01 % Triton
X-100
5 μL
5 μL
• New pipette tips are pre-wetted
with the liquid to be aspirated.
Prewetting option is set (see p. 62).
• Dispensing the liquid with a
greater distance to the calculated
plane surface of the liquid (4 to 5
mm) than liquid type Protein.
• All other data such as liquid type
Protein
• Recommended for nutrient media.
Rinse
Demineralized
water.
Water with low
surfactant
content.
1 μL
3 μL
• Low aspiration and dispensing
speed, strongly delayed blow-out.
• Other data such as liquid type
Water.
• This liquid type is suitable if the
mixing parameters of a transfer
command or the Mix command
are used.
• Can increase the risk of
contamination for small vessels,
e.g., PCR plates.
• Recommended in combination
with the Mix option for reducing
the residual liquid in the pipette
tip.
Speed_xl
Demineralized
water.
1 μL
3 μL
• Mixing at high dispensing speed.
• High dispensing speed poses a
high risk of contamination for
small vessels.
• Good mixing in DWP 96 with
750 μL aspiration and 750 μL
dispensing.
Speed_xs
Demineralized
water.
1 μL
3 μL
• For slow aspiration from filter
plates.
• Very low aspiration speed.
Sediment will not be dispersed.
Water
Demineralized
water.
1 μL
3 μL
• Recommended for most
applications.
• Technical data on the systematic
and random errors were
determined using this liquid type.
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7.3.1.6 Changing the liquid type
Changes to the liquid type have effects on the accuracy of pipetting. Changing the settings
may increase the dispensing deviations.
Abb. 7-14: Sample transfer > Liquid types
Fig. 7-14: Sample transfer > Liquid types
 To change the liquid type, check the Change parameter checkbox.
 Enter the aspiration speed of the liquid in the Aspiration speed field: 0.2 mm/s – 110 mm/s range.
For viscous solutions and large aspiration volumes, select a low aspiration speed. The liquid aspiration
is delayed. The liquid aspiration must be ended before the pipette tip withdraws from the liquid. Select
the low aspiration speed during phase separations or to prevent the distribution of sediments or
particles.
 Enter the dispensing speed of the liquid in the Dispense speed field. Input range: 0.2 mm/s – 110 mm/s
Select a low dispensing speed if you dispense larger volumes in empty vessels. A lower dispensing
speed decreases the risk of liquid spraying back. Use larger vessels to achieve better mixing results due
to a higher speed.
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 Enter the blow-out delay in the Blow delay field. Input range: 0 ms – 99999 ms.
The delay is the break between the end of the dispensing during liquid dispensing and blow-out. This
setting has an effect on the Transfer type Pipette.
If you use liquids with high wetting behavior (e.g., solutions which contain proteins or detergents,
organic solvents) and delayed drain-off characteristics (highly viscous liquids), delay the blow-out. The
residual liquid collects in the lower part of the tip prior to the blow-out.
If you use liquids with low wetting behavior (e.g., water, saline solutions), set the blow-out delay to 0.
 Enter the dispensing speed of the blow-out in the Blow speed field. Input range: 0.2 mm/s – 110 mm/s.
Using liquids that tend to form bubbles on the outlet of the pipette tip increases the dispensing speed of
the blow-out. Decrease the dispensing speed if liquid sprays back into the vessel. This setting has an
effect on the Transfer type Pipette.
 In the Blow movement field, enter the path of the piston during blow-out. Input range: 0 % – 100 % as
percentaged value of the setting Initial stroke.
No blow-out will be carried out during when 0 is entered. The entire path of the piston is part of the
dispensing step.
When entering 100, the entire piston path is available for the blow-out. The blow-out is conducted using
the Blow delay and Blow speed parameters.
Increase and decrease the Blow movement along with the Blow speed setting. This setting only has an
effect on the Transfer type Pipette.
 In the Initial stroke field, enter the path the piston takes before the liquid aspiration. Input range: 0 % –
100 % of the available path, depending on the type of dispensing tool.
The higher the value, the larger the amount of piston path that is available for the blow-out after liquid
dispensing. To decrease the amount of residual liquid in the pipette tip after dispensing, increase the
value. To decrease the formation of foam during dispensing, decrease the value. Reducing the value can
increase the dispensing deviation. The entry 0 means that no blow out will be conducted.
If you change the value, the pipette tips will be replaced before liquid aspiration.
 In the Prewetting field, enter the number of piston movements for pre-wetting the pipette tips. Input
range: 0 – 9 cycles.
Non-wetted pipette tips can be pre-wetted to create the same conditions for dispensing.
Wetting the pipette tips:
• For liquids with low vapor pressure.
• For liquids with reduced surface tension and delayed drain-off characteristics.
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7.3.1.7 Testing amended liquid types on the epMotion
Prerequisites
• The parameters of the liquid types are customized to your requirements.
1. Carry out the sample run of the application with demineralized water.
2. Check the precision and accuracy of the dispensing.
3. Check the working volume of the labware. No liquid may spray out of the labware.
4. Carry out the sample run of the application using the provided liquid.
5. Check the precision and accuracy of the dispensing.
6. Check the working volume of the labware. No liquid may spray out of the labware.
7.3.2
Reagent transfer command
The Reagent transfer command is used to transfer a liquid from one or more source positions into several
destination positions. After the first source position has been emptied, the epMotion switches to the next
source position.
Abb. 7-15: Principle of the Reagent transfer command
Fig. 7-15: Principle of the Reagent transfer command
When using multiple source positions and the optical sensor is switched on , the volume of the first source
position will be checked when starting the application run. If there is not enough liquid available for the
number of samples, the Checkrun window opens. To incorporate the next source position in the calculation,
select accept level and continue. The optical sensor checks the next source position. The calculated volumes
will be summed. The application will start if the volume is sufficient.
The Number of samples command is used to define the number of destination vessels.
The parameters of the command Reagent transfer are identical to the command Sample transfer (see p. 54).
If the Pierce settings function is activated in the properties of the labware, the labware will be opened before
the liquid transfer. If the sealing of a source vessel and the sealing of a destination vessel are to be opened,
the source vessel is opened first. To open the vessels, epBlue uses the same dispensing tool and the same
pipette tips as for liquid transfer.
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Proceed as follows to define the pattern for the Reagent transfer command:
1. Select the source position in the source labware. If you are using several source positions, select these
vessels one after the other.
2. Select the destination position in the destination labware.
Source position and destination position for one pattern are the same color.
7.3.3
Dilute command
The Dilute command is used to create serial dilution series, e.g., for PCR standards. A defined volume is
pipetted several times from one vessel to the next.
Abb. 7-16: Principle of the Dilute command
Undiluted Sample
Diluent
Diluent
Diluent
1. Dilution
2. Dilution
3. Dilution
Fig. 7-16: Principle of the Dilute command
The parameters of the command Dilute are identical to the command Sample transfer (see p. 54).
If the Dilute command is carried out within one labware the source positions and destination positions must
not overlap.
If the Pierce settings function is activated in the properties of the labware, the labware will be opened before
the liquid transfer. If the sealing of a source vessel and the sealing of a destination vessel are to be opened,
the source vessel is opened first. To open the vessels, epBlue uses the same dispensing tool and the same
pipette tips as for liquid transfer.
1. Fill the destination positions with a diluent. Use the Reagent transfer command.
2. Define the number of samples to be diluted Using the Number of samples command.
3. In the pattern, define the number of dilution steps. The pattern is limited by a series or column.
4. Enter the options for the Dilute command.
5. To achieve a good mixture of sample and diluent, activate the Mix > Mix after dispensing option.
The sample and diluent will be mixed after each dispensing.
6. To mix the undiluted sample before the first aspiration, activate the Mix before aspirating option.
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Proceed as follows to enter the pattern for the Dilute command:
1. Select the source position of the undiluted sample.
2. Select the destination positions of the dilution steps. The number of positions defines the number of
dilution steps.
3. Repeat the procedure for each source position.
7.3.3.1 Example of a dilution series
This example explains the concept of a dilution series. The example does not describe the full application.
Objective
• 24 samples are in a rack with 24 positions and should be diluted 1:1000.
• The dilution should be completed in 3 program steps with dilutions of 1:10.
• First, a single-channel dispensing tool is used. An eight-channel dispensing tool will then be used to
reach a higher speed.
Procedure
• 200 μL of each of the samples will be transferred to the 96-well plate using the Sample transfer
command. The dilutions are carried out in this plate.
• 225 μL of the diluent will be transferred from the 300 mL reservoir to each of the empty wells of the
96-well plate using the Reagent transfer command.
• 25 μL of the sample will be aspirated from column 1 using the Dilute command and will be mixed with
respectively 225 μL of the diluent in the columns 2, 3 and 4. The mixing process will be repeated twice
in the following columns.
In this example, each dilution step is a 1:10 dilution. The required 1:1000 dilution will be achieved with the
third 1:10 dilution. The volume aspirated from the undiluted sample also applies for the dilution steps.
Creating dilution series
Transferring undiluted samples to the destination labware
1. Pattern of the Sample transfer command:
The source position and destination position are the same color. epBlue detects the pattern and
suggests the next field.
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Transferring diluent to the destination labware
2. Pattern of the Reagent transfer command:
With an eight-channel dispensing tool, the Reagent transfer command is used to aspirate the diluent
from the 300 mL reservoir and dispense it into the empty wells of the 96-well plate.
Carrying out the dilution
3. Pattern of the Dilute command:
Using an eight-channel dispensing tool, liquid is removed from column 1 and mixed with the diluent in
columns 2, 3 and 4. This procedure will be repeated in the following columns.
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7.3.4
Pool command
The Pool command can be used to combine liquids from several source positions in one or more destination
positions.
Abb. 7-17: Principle of the Pool command
Fig. 7-17: Principle of the Pool command
The parameters of the command Pool are identical to the command Sample transfer (see p. 54).
If you are using the Multiaspirate function, liquid will be removed from the source positions until the
pipette tip is filled. The contents will then be dispensed in the destination position. In the pattern, you
define which source positions will be pooled in the destination position.
The Number of samples command before the Pool command defines the number of source positions.
Options > Change Tips is set to before asp. for next destination well.
If the Pierce settings function is activated in the properties of the labware, the labware will be opened before
the liquid transfer. If the sealing of a source vessel and the sealing of a destination vessel are to be opened,
the source vessel is opened first. To open the vessels, epBlue uses the same dispensing tool and the same
pipette tips as for liquid transfer.
7.3.5
Pool one destination command
The Pool one destination command can be used to combine liquids from several source positions in one
destination position. This command is a simplified Pool command.
The parameters of the command Pool one destination are identical to the command Sample transfer (see
p. 54).
The Number of samples command before the Pool one destination command defines the number of source
positions.
The Options > Change Tips option is set to when command finished. The pipette tips are ejected at the end of
the command.
If the Pierce settings function is activated in the properties of the labware, the labware will be opened before
the liquid transfer. If the sealing of a source vessel and the sealing of a destination vessel are to be opened,
the source vessel is opened first. To open the vessels, epBlue uses the same dispensing tool and the same
pipette tips as for liquid transfer.
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7.3.6
Pierce command
You can open the sealing of individual positions of a labware. The labware must be sealed with suitable
aluminum foil. The epMotion opens the sealing with a single-channel dispensing tool.
You can open the sealing of individual positions using the Pierce command. A liquid transfer does not have
to be performed directly after the Pierce command.
epBlue saves, on which positions of the labware the sealing was opened. This way, the sealing of each
position of the labware can only be opened once.
You have to activate the Pierce settings function in the properties of the labware to open the sealing with the
Pierce command. You have to adapt the parameters of the Pierce settings function to the labware
combination and the aluminum foil .
Abb. 7-18: Pierce > Options command
Fig. 7-18: Pierce > Options command
Pipet. tool checkbox
Select dispensing tool
Racks checkbox
Select Labware
Pattern button
Select Pattern mode to open the labware
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Abb. 7-19: Pierce > Parameter command
Fig. 7-19: Pierce > Parameter command
Change tips radio button
re-use tips checkbox
when command finished Replace pipette tips if all
Restored pipette tips will be reused.
positions have been opened.
The pipette tips will be reused within an
after each perforation Replace pipette tips if a
application for the same source vessel.
position has been opened.
Is only executed if the restore tips option was
keep tips Do not replace pipette tips.
selected in a previous command and the restored
tips are available.
restore tips checkbox
The re-use tips checkbox is only available with
Pipette tips are restored to the position of the box
specific settings of the Change Tip option.
they were taken from.
Is only executed if the tips within an application
are reused for the same source vessel.
The restore tips checkbox is only available with
specific settings of the Change Tip option.
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7.3.7
Comment command
Abb. 7-20: Comment command
Fig. 7-20: Comment command
Comment field
Enter comment
A comment can be output using the Comment command. The comment will be shown when the command
is executed.
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7.3.8
Exchange command
The command Exchange is only available for epMotion 5070.
Abb. 7-21: Exchange command
Fig. 7-21: Exchange command
Labware drop-down menu
Labware taken out of the epMotion
Exchange with drop-down menu
Labware to be placed inside the epMotion
The Exchange command can be used to exchange labware. When the command is executed, epBlue
prompts you to replace the labware manually.
7.3.9
Magnetic separation command
The Magnetic separation command only is available for devices with magnetic finger module.
The Magnetic separation command can be used to separate magnetic beads using the magnetic finger
module.
The Magnetic separation command can only be executed if the PrepRack is positioned on the Eppendorf
ThermoMixer module of the epBlue worktable.
The efficiency of the magnetic separation depends on the properties of the used beads. The Duration
should be at least 2 min.
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If the Magnetic separation command is executed when the Separation On checkbox is checked, the
magnetic fingers will be drawn out. During the waiting period, the magnetic beads will be separated by the
drawn out magnetic fingers on the tube inner wall. After the waiting period has elapsed, the next command
in the procedure will be executed. If the Magnetic separation command is executed when the Separation On
checkbox is not checked, the magnetic fingers will be drawn in.
Abb. 7-22: Magnetic separation command
Fig. 7-22: Magnetic separation command
Separation enabled checkbox
Check the checkbox to start the separation
Uncheck the checkbox to end the separation
Duration field
Waiting period. The application stops until the
waiting period has elapsed.
Starting the separation
 To start the separation, execute the Magnetic separation command when the Separation On checkbox is
activated.
The magnetic fingers will be drawn out.
Exiting the separation
 To end the separation, execute the Magnetic separation command when the Separation On checkbox is
deactivated.
The magnetic fingers are drawn in.
7.3.10
Mix command
The Mix command can be used to mix liquids in a position by multiple aspiration and dispensing steps.
The options of the Mix command are identical with the options of the Sample transfer command in the Mix
area (see p. 58).
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7.3.11
Number of samples command
The Number of samples command is used to define how many samples or positions will be processed in the
following transfer commands. The Number of samples command is valid until it has been replaced by a new
Number of samples command.
You can use several Number of samples commands within one application. When the application starts, the
software will query entries for the commands Number of samples. If you do not want to execute part of the
application, enter 0.
If you do not use the Number of samples command, epBlue asks you for the number of samples at the start
of the application. This entry then applies for all commands of the application.
The number of samples is limited by the pattern.
Abb. 7-23: Number of Samples command
Fig. 7-23: Number of Samples command
Fix Sample Count checkbox
Max Number of Samples field
Check the checkbox in order to define the number of
Active if the Fix number of samples checkbox is
samples
not checked. Maximum number of samples
Uncheck the checkbox to enter the number of
that can be processed in the application.
samples at the start of the application.
Comment field
Number of Samples field
This text field is displayed when the software
Active if the Fix number of samples checkbox is
asks for the number of samples. Enter
checked. Enter the number of samples. This value will
explanatory information in the field.
be taken into account for the pattern.
In accordance with the subsequent command, the Number of samples command has the following
effects:
• Prior to the Sample transfer command: Number of samples aspirated from the source labware.
• Prior to the Reagent transfer command: Number of destination positions liquid is dispensed into.
• Prior to the Pool or Pool one destination command: Number of source positions from which liquid is
removed.
• Prior to the Dilute command: Number of samples to be diluted. The number of dilution steps is defined
by the pattern.
• Prior to the Mix command: Number of source positions in which liquid is mixed.
• With an eight-channel dispensing tool: 8 samples are processed for values 1 – 8. For values 9 - 16, 16
samples are processed, etc.
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7.3.12
Temperature command
The command Temperature only is available for devices with thermal module.
With the Temperature command, you can temper the labware in a thermal module. The temperature control
stops when epBlue is exited.
Abb. 7-24: Temperature window
Fig. 7-24: Temperature window
Location drop-down list
Select a thermal module.
Keep Temperature after application run checkbox
Activate the thermal module for the period after
the application has exited. This function can be
used to cool samples after the application run has
been completed.
Temperature on checkbox
Activate the thermal module. Activated thermal
modules are switched off when you uncheck the
checkbox.
Temperature field
Set the set temperature of the thermal module.
0 °C – 110 °C range.
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7.3.13
ThermoMixer command
The ThermoMixer command is only available for devices with Eppendorf ThermoMixer
module.
The ThermoMixer command can be used to set the temperature and speed of the Eppendorf ThermoMixer
module.
Abb. 7-25: ThermoMixer window
Fig. 7-25: ThermoMixer window
Template drop-down list
The labware library contains templates for the
Eppendorf ThermoMixer module.
Temperature on checkbox
Activate the tempering of the Eppendorf
ThermoMixer module.
Mixing on checkbox
Activate the mixing process of the Eppendorf
ThermoMixer module.
Keep temperature after applicaton run field
Activate the temperature control for the period
after the end of the application. This function can
be used to cool samples or reagents after the end
of the application.
Speed field
Set the mixing frequency. 300 rpm – 2000 rpm
range.
Duration field
Set the mixing time. 5 s – 120 min range.
Temperature field
Set the set temperature of the Eppendorf
ThermoMixer module, 4 °C – 95 °C range.
Information on the Eppendorf ThermoMixer module can be found in the epMotion hardware
operating manual.
NOTICE! Contamination of samples and the device due to excessive speeds.
If the speed is set too high, liquid sprays out of the vessels. Labware may become loose from
the holder and fly around.
 Observe the labware maximum speeds.
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Tab. 7-2:
Maximum speed for labware on the Eppendorf ThermoMixer module
Labware
Maximum speed in rpm
Vessels
1000
Deepwell plates with 96 wells
1200
Deepwell plates with 384 wells
1200
Microplate with 6 wells
1000
Microplates with 96 wells
2000
Microplates with 384 wells
2000
PCR plate with 96 wells
2000
PCR plate with 384 wells
2000
ReservoirRack
Mixing not possible
Thermorack or Thermoblock
1000
Thermoblock 96 with a semi-skirted PCR plate for 250 μL
1700
Thermoadapter for Microplate 96/U/V with Eppendorf Microplate 96/V
1600
Thermoadapter for Microplate 96/U/V with Eppendorf Microplate 96/U
1600
Thermoblock PCR 96 OC
1700
Thermoadapter for 96 wells
1000
Thermoadapter for 384 wells
1000
Thermorack TMX
1300
PrepRack
1300
7.3.13.1 Creating a template for the Eppendorf ThermoMixer module
Prerequisites
• The ThermoMixer command is selected (see p. 76).
1. Edit the template in the Options field.
The word Changed appears next to the Save button.
2. Enter the name of the template.
3. Click on the Save button.
The template is saved.
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7.3.13.2 Managing templates for the Eppendorf ThermoMixer module
Abb. 7-26: Labware Editor > TMX Templates
Fig. 7-26: Labware Editor > TMX Templates
 Manage the templates for the Eppendorf ThermoMixer module in the Labware Editor > TMX Templates
folder.
7.3.14
Vacuum command
BAR
The command Vacuum only is available for devices with vacuum unit.
The Vacuum command is used to create a vacuum in the vacuum unit. You can aspirate liquid in filter
plates.
Prior to executing the application, equip the vacuum unit as described in the hardware operating manual.
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Abb. 7-27: Vacuum window
Fig. 7-27: Vacuum window
Frame drop-down list
Vacuum frame used.
Use Vacuum Lid checkbox
Activate transport of the Vac Lid with the gripper.
Pressure field
Vacuum in the vacuum unit. Unit mbar or kPa.
Maximum underpressure 850 mbar/85 kPa.
Use Vacuum Lid drop-down list
Vac Lid used.
Duration field
Time during which the vacuum is maintained. 0 s
– 100 min 59 s range.
Check levels checkbox
The optical sensor checks the remaining volume
of all wells in a filter plate on the vacuum unit. If
remaining liquid has been detected, a vacuum
will be reapplied. Checking the remaining volume
takes too long.
7.3.14.1 Transporting the Vac Lid with the gripper
Use the Vacuum command to transport the Vac Lid from location T0 to the filter plate in the vacuum unit
and back to location T0 with the gripper.
Prerequisites
• The Vac Lid is in location T0.
 To have the Vac Lid transported from location T0 to the filter plate at the start of the Vacuum command,
activate the Use Vacuum Lid checkbox.
After the time set in the Duration field has elapsed, the gripper takes the Vac Lid off the filter plate at an
underpressure of 100 mbar (10 kPa). The gripper transports the Vac Lid to position T0.
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7.3.15
Transport command
The command Transport only is available for devices with gripper.
The Transport command can be used to transport labware. The gripper takes labware from one location and
transports it to another location.
Abb. 7-28: Transport window
Fig. 7-28: Transport window
Labware
Select the labware.
To location
Select a location.
Information on the labware that is being transported can be found in the hardware operating
manual.
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7.3.16
User intervention command
The User intervention command is used to interrupt an application. The application will be continued when
the user confirms a message.
You can use the User intervention command to empty the waste container manually.
Do not change the filling volume during the interruption. If you need to change the filling
volume, use several applications to do so.
Do not change the equipping of the worktables.
Abb. 7-29: User Invention window
Fig. 7-29: User Invention window
Comment window
The comment appears when the command is
executed.
Alarm checkbox
An acoustic signal sounds if the command is
executed.
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7.3.17
External process command
With the External process command, labware can be removed from the device. The labware can be further
processed outside of the epMotion. The application waits until the labware is reset.
Abb. 7-30: External process command
Fig. 7-30: External process command
Labware drop-down list
Select labware to be removed.
Rescan location checkbox
The optical sensor checks the position of the
restored labware.
Verify barcode checkbox
The barcode of the restored labware is checked
manually.
Comment field
Enter comments
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7.3.18
Wait command
The Wait command is used to create a waiting period. The application will be continued after the waiting
period. The command can be used to take into account incubation periods between the addition of
reagents.
Abb. 7-31: Wait window
Fig. 7-31: Wait window
Duration field
Waiting period.
Wait for temperature checkbox
The waiting period begins after the thermal
module has reached the set temperature.
7.4
Location drop-down list
Thermal module whose temperature control is
waited for.
Working with CSV files
A CSV file is an ASCII text file. A CSV file defines the structure and contents of a table. Every line of text in
a CSV file corresponds to a row in the table. The columns in the table are separated by commas,
semicolons, or tabs.
You can import CSV files in epBlue. This way you can import commands for liquid transfer and the volume
of the used labware.
When creating a CSV file for epBlue, you need the following information:
• Volume
• Source vessel
• Destination vessel
• Dispensing tool
• Optional ID
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7.4.1
Creating CSV files for liquid transfer
You can use the CSV files to carry out liquid transfers with various volumes. You can dilute samples with
various concentrations to a uniform concentration (normalization).
Abb. 7-32: CSV file in a spreadsheet software and a text editor
Fig. 7-32: CSV file in a spreadsheet software and a text editor
Each transfer command is defined in a designated line.
Line 1 contains the header for the transfer command. In the figure these are cells A1 to E1.
• Labware
• Source barcode
• Source List Name
• Destination Barcode
• Destination List Name
• A labware has been specified for the transfer. In the figure this is cell A2.
• If more than one set of labware is used, the labware must be numbered. Enter the numbers in row A
starting at cell A2. Example: Number labware 2 with digit 2. Enter digit 2 in cell A3.
Line 7 contains the header for the labware. In the figure these are cells A7 to H7.
• ID: ID of the labware
• Labware: source labware
• Source: position in the source labware
• Labware: destination labware
• Destination: position in the destination labware
• Volume: transfer volume in μL
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• Tool: dispensing tool
• Name: name of the sample
If you do not have the epBlue ID module, you can leave the following fields empty:
• Source barcode
• Source List Name
• Destination barcode
• ID
The ID column must always be present in the CSV file.
• The CSV file has 8 columns.
• Each line in the CSV file must have 7 separating characters. Empty columns are separated by separating
characters.
• The values start in line 8. They are entered without interruption in the following lines.
• The values are separated by commas, semicolons, or tabs.
• Decimals are divided by a dot or comma.
• The separating characters for the values and decimals may not be identical.
• There may be no entries below the values. These entries are interpreted as commands during the import
and cause errors.
• Rows that start with # are interpreted as comments. These rows are not imported.
• Positions in microplates and PCR plates can be specified numerically or alphanumerically. The rows are
specified first, then the columns.
• Positions in racks are named the same as in the labware definition.
• Dispensing tools are coded as in the epBlue Application Editor, e.g. TS_50, TM_50_8. All dispensing
tools can be used.
7.4.2
Importing a CSV file for liquid transfer
Prerequisites
• The CSV file has been created (see p. 84).
1. Create a new application.
2. Insert the Sample transfer command.
The Sample transfer command defines the settings for all commands from the CSV file.
3. In the command Sample transfer > Parameters, determine the locations for source labware and
destination labware.
The locations defined in the command Sample transfer are available for the commands in the CSV file.
In the example, one location each was defined for the source labware and destination labware. These
locations are available for commands in the CSV file.
If two sets of labware are specified in the CSV file (line A2-A3), two sets of source labware and two sets
of destination labware must be defined in the transfer command. This also applies if only one set of
source labware is used in the transfer command, e.g. for the transfer of a source labware into two sets of
destination labware.
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The labware from the CSV file must correspond to the parameters of the command Sample transfer.
Labware is used in the sequence defined in the Sample transfer command. If rack 2 is specified in the
CSV file in column B, the second rack from the Source area will be used as the source vessel for this
command.
4. Define options for transfer steps from the CSV file in the fields Options and Mix.
The Elution from filter option is not available for imported commands.
The mixing volumes and mixing frequencies must be suitable for all imported transfer commands. The
value set for the mixing frequency must be manually overwritten with a different value. If you use
different dispensing tools, use a mixing frequency of 11 mm/s.
5. Check all settings.
The settings must meet all of the procedure requirements.
6. Click on the Sample transfer command.
7. Click on the
CSV
symbol in the menu bar.
8. Select the CSV file. Click on the Open button.
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The CSV import options window opens.
9. Select how the CSV file is to be imported.
Pipette (without optimization)
The CSV file is implemented in the defined order into transfer steps in the
Pipette dispensing mode.
Pipette
The CSV file is implemented into transfer steps in the Pipette dispensing
mode.
The command is optimized. The optimization is realized according to the
order in which source vessels and dispensing tools are used. The use of
pipette tips and the time for executing the command are reduced.
Multidispense
Replicates with the same volume are dispensed from one source vessel.
The command is optimized. The optimization is realized according to the
order in which source vessels and dispensing tools are used. The use of
pipette tips and the time for executing the command are reduced.
10. Press the OK button.
The CSV file is imported. The commands are imported into the procedure. The procedure is shown in
the Procedure column.
7.4.3
Creating CSV files for the filling volume
You can create and edit CSV files using a spreadsheet software or text editor.
Each line contains information on a position of the labware. The position and the filling volume in the
position are specified.
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Abb. 7-33: CSV file in a spreadsheet software and a text editor
Fig. 7-33: CSV file in a spreadsheet software and a text editor
1. In the text editor, enter the terms Position and Volume in the header.
2. In the spreadsheet software, enter the term Position in cell A1. Enter the term Volume in cell B1.
3. Specify the position in the labware.
The names of the positions are shown in the Application Editor.
4. Specify the filling volume of the position in μL.
7.4.4
Importing CSV files for the filling volume
Prerequisites
• The application has been opened in the Application Editor.
• The CSV file has been created (see p. 87).
1. Select the labware on the epBlue worktable.
2. Open the window with the labware properties.
3. Select Import all volumes.
4. Select the CSV file.
5. Press the OK button.
The CSV file is imported.
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7.5
Pattern
For the pattern, enter the source position and destination position between which liquid transfers take
place.
Type of pattern
Description
Regular pattern
•
•
•
•
Available for all commands.
With regular pattern.
epBlue detects and completes the pattern.
Not available for ReservoirRacks and ReservoirRack modules.
Irregular pattern
•
•
•
•
Available for some commands.
Pattern according to a random pattern.
epBlue cannot detect and complete the pattern.
Replicates not possible.
Whether you can define regular or irregular patterns depends on the command and the labware.
Tab. 7-3:
Pattern options with regular labware
Command
Source labware pattern
Destination labware pattern
Sample transfer
Regular and irregular
Regular and irregular
Reagent transfer
Regular and irregular
Regular and irregular
Pool
Regular
Regular and irregular
Pool one destination
Regular and irregular
Regular and irregular
Dilute
Regular and irregular
Regular
Mix
Regular and irregular
Regular and irregular
Tab. 7-4:
Pattern options with a ReservoirRack
Equipped with ReservoirRack module for
Pattern
ReservoirRack
Reservoir 30 mL
Reservoir 100 mL
Regular
ReservoirRack
Individual vessels
Irregular
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7.5.1
Menu bar in the Edit Pattern window
Abb. 7-34: Menu bar in the Edit Pattern window
Fig. 7-34: Menu bar in the Edit Pattern window
Reset
Reject pattern.
Back arrow
One step back.
Complete
Regular patterns are completed automatically.
The pattern is ended when the number of
samples defined in the Number of Samples
command is reached.
Preview
Preview of the pattern with an increased number
of samples.
Show
Show the pipetting sequence.
Next arrow
One step forward.
7.5.2
Defining regular patterns
Prerequisites
• Labware is positioned on the worktable.
• Transfer command or Mix command activated.
1. Select the source labware in the Source area.
2. Select the destination labware in the Destination area.
3. Click the Pattern button.
The Edit Pattern window appears.
4. To delete an existing pattern, press the Reset button.
If you work with an eight-channel dispensing tool, select the top position. For a 96-well pate,
this is row A. For a 384-well plate, this is rows A or B. epBlue adds the pattern for the
remaining channels.
5. Select the first source position in the source labware.
6. Select the first destination position in the destination labware. To create replicates, select several
destination positions one after the other.
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Abb. 7-35: Selecting the first source position
Abb. 7-36: Selecting the first destination position
Fig. 7-35: Selecting the first source position
Fig. 7-36: Selecting the first destination position
7. Select the next source position in the source labware.
8. Select the next destination position in the destination labware.
Abb. 7-37: Selecting the next source position
Fig. 7-37: Selecting the next source position
Abb. 7-38: Selecting the next destination position
Fig. 7-38: Selecting the next destination position
epBlue attempts to detect the pattern and marks the next position gray.
If you created replicates during the first step, and select the destination position for the second liquid
transfer, the positions are defined for the replicates in the same order as in the first step.
9. If the pattern matches your requirements, press the Complete button.
The pattern is completed automatically up to the defined number of samples.
If you would like to discard the pattern, press the Reset button.
10. To check a pattern, press the Show button.
The order of the pattern is displayed. The source position and destination position of a liquid transfer
are the same color.
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7.5.3
Defining irregular patterns
Irregular patterns can be defined for source labware and destination labware. If the pattern is irregular, it is
not completed.
Prerequisites
• Labware is positioned on the worktable .
• Create the application with the Transfer or Mix command (see p. 54).
1. Click the Pattern button.
Abb. 7-39: Edit Pattern window
Fig. 7-39: Edit Pattern window
The Edit Pattern window appears.
2. Check the Source checkbox in the Irregular pattern area.
3. Optional in the Destination area: check the Irregular pattern checkbox.
4. Define the pattern. Specify all source positions and destination positions.
If the pattern is irregular, the software does not complete the pattern automatically. The Complete
button is not active.
epBlue Application Editor - Creating and editing applications
epBlue™ with MultiCon
English (EN)
7.6
Optical sensor
The principle of operation of the optical sensor is described in the hardware manual.
The optical sensor has the following functions:
Measure fill level in vessels
• The optical sensor determines the filling level of the labware positions. There are 2 ways of doing this:
– The optical sensor determines the filling level in all positions used.
– The optical sensor determines the filling level in the first position, in the last position and in 8
randomly determined positions.
– The optical sensor cannot measure the fill level in the following labware: 384-well plates, Universal
Disc from Focus Diagnostics, Qiagen rotor disc 72, 100.
Detect pipette tips
• The optical sensor determines the quantity and the positions of the ep.T.I.P.S. pipette tips. Motion
pipette tips in TipHolders and Racks by Eppendorf.
Check labware on the worktable
• The optical sensor scans the code on the labware.
• epBlue checks if the labware on the epMotion worktable matches the labware on the epBlue worktable.
If the optical sensor is switched off, the processing time of the application is reduced.
7.6.1
Measuring the filling level of the labware
The Detect volumes function enables you to measure the filling volume in the labware positions
The optical sensor detects the filling volume up to the detection limit. The detection limit depends on the
labware geometry. Generally, levels of 3 mm or higher can be detected. Each labware definition contains
information on the detection limit of the filling volume. If the filling volume in one position is below the
detection limit, you have to enter the filling volume manually.
The optical sensor can execute the Detect volumes function based on different parameters. You define these
parameters as application parameters when you create an application. When you start a run, you can
change the application parameters. You can define individual run parameters for each run.
Application parameters
Run parameter
Defined in the Application Editor.
Defined in the Application Runner.
Defined when creating the application (see p. 94)
Defined when starting the run (see p. 96).
Defined individually for each labware used in the
application.
Applies for the complete labware of the application.
Different parameters can be defined for each run.
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7.6.1.1 Defining application parameters for measuring the filling volume of plates
Abb. 7-40: Properties window for plates
Fig. 7-40: Properties window for plates
Tab. 7-5:
Parameters of the Detect volumes function
Off
• Deactivate the optical sensor.
• The filling volumes of all positions must be entered when the application is started.
For labware with ≤ 56 positions, the filling volume for each position can be specified in
the Volume column.
For labware with > 56 positions, one filling volume can be specified for all positions.
This filling volume is recommended at the start of the application, and can be changed.
• The volume specification does not take into account the surface meniscus of the liquid.
• If the filling volume is not enough, the result may be faulty dispensing.
All positions
• Checks the filling volume in all positions used in the application.
• If you work with a single-channel dispensing tool, epBlue uses the calculated filling
volume for each position.
• If you work with a multi-channel dispensing tool, the epBlue uses the lowest calculated
filling volume in a column for liquid aspiration and the highest calculated volume in a
column for liquid dispensing.
Random
positions
• The optical sensor checks the filling volume of the first, last and 8 randomly selected
positions.
• For the parameter to be available, the labware has to have at least 24 positions.
If the filling volumes in one plate differ significantly or the labware has very low filling
volumes, use the Aspirate from bottom and Dispense from top options.
 To set the application parameters for the Detect volumes function (see To change the properties of a
labware, double-click on the labware. on p. 46).
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7.6.1.2 Application parameters for measuring filling volume of special labware
Abb. 7-41: Properties window for special labware
Fig. 7-41: Properties window for special labware
The optical sensor cannot measure the filling level in the following labware:
• 384-well plates
• Universal Disc by Focus Diagnostics
• Qiagen Rotor-Disc 72 and Qiagen Rotor-Disc 100
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7.6.1.3 Defining the run parameters for measuring the filling volume
Abb. 7-42: Run parameters when starting the application
Fig. 7-42: Run parameters when starting the application
Detect volumes radio button
Input volumes manually radio button
The optical sensor measures the filling volume.
Enter the filling volume manually.
In which positions the optical sensor measures
Detect tips slider
the filling volume is defined in the labware
Check-in which positions pipette tips are located
properties.
in the labware.
The settings made in the labware properties are
overwritten with the run parameters.
Check labware placement slider
Check the labware on the worktable.
Use required minimum volume radio button
epBlue assumes that all vessels are filled with the
minimum required volume for this run.
 To set the run parameters for the Detect volumes function (see Setting the run parameters for the optical
sensor on p. 28).
Examples:
Prerequisites
• Application parameters Off
• Run parameter Detect levels
 The filling volume is measured at the start of the application.
Prerequisites
• Application parameters All positions
• Run parameter Input level manually
 The filling volume must be entered manually when the application is started.
epBlue Application Editor - Creating and editing applications
epBlue™ with MultiCon
English (EN)
7.6.2
Detecting pipette tips
You can only use the Detect tips function, if you are using epT.I.P.S. Motion in TipHolders and racks by
Eppendorf.
Enter the Detect tips function as a run parameter at the start of the application (see Setting the run
parameters for the optical sensor on p. 28).
The Detect tips function checks the type of pipette tips and determines the number and position of tips in
the rack.
The epT.I.P.S. Motion tray is coded. The optical sensor uses the code to detect the type of pipette tips.
Arrangement of pipette tips if the Detect tips function is activated.
Tab. 7-6:
1
2
3
4
5
6
7
8
9
10
11
1
12
2
3
4
5
6
7
8
9
10
11
1
12
A
A
A
B
B
B
C
C
C
D
D
D
E
E
E
F
F
F
G
G
G
2
3
4
5
6
7
8
9
10
11
12
The Detect tips function checks to see if there are enough pipette tips available for the application. The
pipette tips must be continuously positioned without gaps. epBlue prompts you to refill the pipette tips
after the available tips have been depleted. In the next application, you can continue using a rack that has
already been opened.
Arrangement of pipette tips if the Detect tips function is deactivated.
Tab. 7-7:
1
2
3
4
5
6
7
8
9
10
11
12
1
2
3
4
5
6
7
8
9
10
11
1
12
A
A
A
B
B
B
C
C
C
D
D
D
E
E
E
F
F
F
G
G
G
2
3
4
5
6
7
8
9
10
11
12
If the Detect tips function is deactivated, the columns of the rack must be fully equipped. The tips must be
available as of position A1.
7.6.3
Checking the labware on the worktable
Enter the Check labware placement function as a run parameter when starting the application (see Setting
the run parameters for the optical sensor on p. 28).
The Check labware placement function detects the labware coding. The function checks if the labware on
the epMotion worktable matches the equipment of the epBlue worktable.
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epBlue Application Editor - Managing applications and folders
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8
epBlue Application Editor - Managing applications and folders
This chapter describes how to manage applications in epBlue. Folders are managed according to the same
rules.
8.1
Menu bar and start screen
Abb. 8-1: Application Editor menu bar
Fig. 8-1:
Application Editor menu bar
Creating a new application
Cutting an application or folder
Creating a new folder
Pasting an application or folder
Opening an application
Deleting an application or folder
Editing the application properties
Importing an application
Copying an application or folder
Exporting an application
Abb. 8-2: Start screen in the Application Editor
Fig. 8-2:
Start screen in the Application Editor
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8.2
Copying and deleting applications
8.2.1
Copying applications
You can copy applications.
The Eppendorf folder contains applications defined by Eppendorf AG. These applications are
used as templates and program examples.
 Copy this application into your area in order to modify and execute it.
Prerequisites
• The start screen in the Application Editor is open.
1. Select the desired application.
2. Click on the
symbol in the menu bar.
3. Select the folder in which the application is.
4. Click on the
symbol in the menu bar.
The application appears in the selected folder.
8.2.2
Deleting an application
You can delete an application.
Prerequisites
• The start screen in the Application Editor is open.
1. Select the application.
2. Click on the
symbol in the menu bar.
3. Confirm deleting the application. To do this, press the OK button in the message window.
The application is deleted.
8.3
Changing the properties and name of the application
You can change the name and properties of an application.
Prerequisites
• The start screen in the Application Editor is open.
 Select the application.
The properties of the application appear in the Properties field.
 Press the
symbol in the menu bar.
The Edit Properties window opens.
epBlue Application Editor - Managing applications and folders
epBlue™ with MultiCon
English (EN)
Abb. 8-3: Edit Properties window
Fig. 8-3:
Edit Properties window
 Change the application settings.
 To save the changes, press the Save button.
8.4
Importing and exporting applications
8.4.1
Importing applications
Tab. 8-1:
You can import applications in the following file formats:
File format
Source of file
*.ws or *.lhs
Applications from the control panel
*.dws
Applications from versions < 20.X of epBlue
*.export
Applications from versions ≥ 20.X of epBlue
Prerequisites
• The start screen in the Application Editor is open.
1. Select the folder into which the application is to be imported.
2. Click on the
symbol in the menu bar.
The Import Applications window opens.
3. Select the applications to be imported.
4. Press the Open button.
The Import Application window opens.
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Abb. 8-4: Import Application window
Fig. 8-4:
Import Application window
Name area
List of all applications for which import was
selected.
Standard application checkbox
Activate if the application is to be imported.
Selected checkbox
Activated, if the application is an Eppendorf
template.
Define import rules area
Define rules for the import of the applications.
Materials tab
Show the labware used in the applications.
Import button
Import applications.
Cancel button
Cancel the import.
5. Define the rules for importing labware.
6. Press the Import button.
The files will be imported. A summary of the import will be displayed.
7. Press the Finish button.
If you import applications from older software versions, the display of the worktable in epBlue
may not match your device configuration. To remedy this problem, save the application under
a new name using Save as.
8.4.2
Exporting applications
You can export applications individually. You can export a folder with applications.
Prerequisites
• The start screen in the Application Editor is open.
1. Select the application to be exported.
2. Click on the
symbol in the menu bar.
The Browse For Folder window opens.
3. Select the folder in which the application is to be saved.
4. Press the OK button.
The applications are exported.
Exported applications can only be used with epBlue from version 40.4.
epBlue Application Editor - Managing applications and folders
epBlue™ with MultiCon
English (EN)
8.5
Saving an application as a PDF file
You can save an application as a PDF file or print the application. An application includes the procedure
and equipping of the worktable. You can save the log of an application as a PDF file or print the log (see
p. 40).
Prerequisites
• A printer is connected to the MultiCon PC.
• The application is open.
1. Click on the
symbol.
The print preview opens. The menu bar contains the following symbols:
Search in the document.
Show the first page.
Print document.
Show the previous page.
Page setup.
Show the next page.
Scale the document.
Show the last page.
Zoom in the view.
Create a PDF.
Set the zoom factor.
Close the view.
Zoom out the view.
2. Check the layout and set up the page.
3. Save or print the document as a PDF file.
4. Close the window.
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epBlue Labware Editor - Creating and managing labware
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9
epBlue Labware Editor - Creating and managing labware
This chapter describes how to create and manage labware and labware combinations in epBlue.
Information on properties and use of labware can be found in your hardware operating manual.
9.1
Overview of labware
The name of the labware includes information on the manufacturer and type. Example: EP_MTP_384V
stands for Eppendorf_microplate_384 wells in V-Form.
Tab. 9-1:
Labware types
Abbreviation
Description
DWP
Deepwell plate
FP
Filter plate
MTP
Microplate
PCR
PCR plate
TP
Plate with vessels that can be removed individually.
Cleanup
Plate from the PCR Cleanup Kit
DNA/RNA
Plate from the kit for purification and insulation
TT
Eppendorf twin.tec
PCR Plate Thermo
Fixed combination of thermoblock and PCR plate
Quantities
Maximum filling volume of a vessel in mL or μL
Tab. 9-2:
Plates
Labware type
Labware
DWP 96
Deepwell plate with 96 wells
DWP 384
Deepwell plate with 384 wells
MTP 6
Microplate with 6 wells
MTP 24
Microplate with 24 wells
MTP 96
Microplate with 96 wells
MTP 384
Microplate with 384 wells
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9.2
Managing labware
9.2.1
Menu bar
Abb. 9-1: Menu bar in the Labware Editor
Fig. 9-1:
Menu bar in the Labware Editor
Create new labware.
Import labware.
Open labware.
Export labware.
Delete labware.
Export several labware at the same time.
Display labware properties.
Abb. 9-2: Start screen of the Labware Editor
Fig. 9-2:
Start screen of the Labware Editor
epBlue Labware Editor - Creating and managing labware
epBlue™ with MultiCon
English (EN)
9.2.2
Deactivating and activating labware
To keep a clear overview of your labware library in the Application Editor, you can deactivate labware that is
not being used. Deactivated labware can be reactivated at any time.
Applications with deactivated labware do not start. If you start an application with deactivated labware, an
error message appears.
Proceed as follows:
1. Open the Labware Editor.
2. Select the labware.
The status of the labware is displayed in the Properties area.
3. To deactivate the labware, flip the switch from active to inactive.
4. To activate the labware, flip the switch from inactive to active.
9.2.3
Deleting labware
You can delete labware that you have created. Proceed as follows:
Prerequisites
• The Labware Editor is open.
1. In the Labware Library, select the labware to be deleted.
2. Check to see if the selected labware is activated.
3. Deactivate activated labware (see p. 107).
4. Click on the
9.2.4
symbol to delete the labware.
Importing labware
You can import labware definitions in zip and export formats. Proceed as follows:
Prerequisites
• The Labware Editor is open.
1. Click on the
symbol in the menu bar.
The Open Labware window opens.
2. Select and highlight the labware.
3. Press the Open button.
The Import labware window opens.
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Abb. 9-3: Import labware window
Fig. 9-3:
Import labware window
Name area
List of the labware selected for import.
Define import rules area
Define rules for the import of the labware.
Standard labware checkbox
Activated, if the labware is standard Eppendorf
labware.
Import button
Import labware.
Selected checkbox
Activate if the labware is to be imported.
Cancel button
Cancel import.
4. Check selected labware definitions. Define rules for the import.
5. To import the labware definitions, click on the Import button.
The labware definitions are imported to epBlue.
Abb. 9-4: Imported labware
Fig. 9-4:
Imported labware
6. End the import by clicking on the Close button.
epBlue Labware Editor - Creating and managing labware
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9.2.5
Exporting labware
You can export labware definitions. You can export individual labware definitions or the content of a folder.
You can use exported labware definitions for example on another epMotion.
Exported labware can only be used with epBlue from version 40.4.
Proceed as follows:
Prerequisites
• The Labware Editor is open.
1. Select labware or folders.
2. Click on the
symbol in the menu bar.
Abb. 9-5: Export labware window
Fig. 9-5:
Export labware window
The Export labware window opens.
3. Select the labware you would like to export from the Selected column.
4. Confirm the selection by pressing Save.
The Browse For Folder window opens.
5. Select the folder where you want to save the labware.
6. Start export. Press the OK button.
The labware is exported to the selected folder in the format .export.
9.2.6
Viewing the properties of the labware
Proceed as follows:
Prerequisites
• The Labware Editor is open.
 Select the labware.
The properties of the labware are displayed in the Properties column.
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9.2.7
Setting the bottom tolerance
NOTICE! Material damage due to placing the pipette tip on the base of the vessel.
If the pipette tips touch the bottom of the tube, the bottom tolerance is too low. The labware
becomes damaged and the dispensing volume is distorted.




Test the application without liquid.
Check the bottom tolerance when exchanging pipette tips, plates, and vessels.
If pipette tips become jammed at the base of the vessel, increase the bottom tolerance.
If deviations in the dispensing volume occur, increase the bottom tolerance.
Proceed as follows:
Prerequisites
• The Labware Editor is open.
1. Select the labware.
2. Click on the
symbol.
The Edit Properties window opens.
Abb. 9-6: Edit Properties window
Fig. 9-6:
Edit Properties window
Name field
Name of labware
Remain Volume field
Remaining volume. Is calculated.
Comment field
Description of labware
Eppendorf Standard Labware checkbox
This checkmark is placed automatically and cannot be
removed. Labware definitions from Eppendorf are write
protected.
Bottom Tolerance field
Bottom tolerance. The lowest
bottom tolerance is 200 μm.
Save button
Save changes.
3. Change the bottom tolerance in the Bottom Tolerance field.
4. Save changes using the Save button.
The settings are saved. If the labware is write protected, a copy will be saved. Copies are numbered
consecutively.
epBlue Labware Editor - Creating and managing labware
epBlue™ with MultiCon
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9.3
Creating labware combinations
Before you create an application, you have to create the required labware combination and save it in your
labware library.
The following options are available to you:
• Equipping racks, thermoracks, or ReservoirRack modules with individual vessels.
• Equipping ReservoirRack with reservoirs or ReservoirRack modules.
• Create a labware combination based on existing labware.
9.3.1
Creating a labware combination.
Prerequisites
• The Labware Editor is open.
Abb. 9-7: Create new labware window
Fig. 9-7:
Create new labware window
Name field
Name of labware
Type option field
Desired labware combination
Comment field
Description of labware
1. Click the
symbol in the menu bar.
The Create new labware window opens.
2. Enter the name, description, and labware combination.
Racks/thermoracks + tubes
Equip the rack and thermorack with individual vessels.
Modules + tubes
Equip the ReservoirRack module with tubes.
ReservoirRack 7
Equip the ReservoirRack 7 with ReservoirRack modules.
ReservoirRack 3
Equip the ReservoirRack 3 with ReservoirRack modules.
3. Click the Create button.
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The labware combination is opened. You can create the labware combination.
Abb. 9-8: Creating labware
Fig. 9-8:
9.3.2
Creating labware
Creating a labware combination based on existing labware
Abb. 9-9: Start screen of the Labware Editor
Fig. 9-9:
Start screen of the Labware Editor
Prerequisites
• The Labware Editor is open.
1. Select the labware.
The Properties column displays information about the labware.
epBlue Labware Editor - Creating and managing labware
epBlue™ with MultiCon
English (EN)
2. Click the
symbol in the menu bar.
The Edit Properties window opens.
Abb. 9-10: Edit Properties window
Fig. 9-10: Edit Properties window
3. Edit the properties of the labware.
4. Enter a new name in the Name field.
5. Press the Save button.
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9.3.3
Equipping racks, thermoracks, ReservoirRack modules with individual vessels
Abb. 9-11: Creating a labware combination
Fig. 9-11: Creating a labware combination
Racks column
Racks and ReservoirRack modules. If you have
selected a rack, only vessels that can be
combined with the rack will be shown.
Clear button
Delete the selected individual vessel, rack,
ReservoirRack module.
Symbol
Tubes column
Close the labware.
Individual vessels. If you have selected an
Symbol
individual vessel, only racks that can be
Save the labware combination as new labware.
combined with an individual vessel will be shown.
Symbol
Properties column
Save changes.
Information on the selected labware
Reset button
Undo changes. Return to the status before the
last save.
Proceed as follows:
Prerequisites
• The labware was created with the Racks and Tubes or Module Rack + Tubes option.
• The labware is open.
1. Select a rack in the Racks column.
2. Select matching vessels in the Tubes column.
epBlue Labware Editor - Creating and managing labware
epBlue™ with MultiCon
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Abb. 9-12: Rack and vessel as labware combination
Fig. 9-12: Rack and vessel as labware combination
3. To save the labware combination, click the
symbol in the menu bar.
The labware combination is saved in the labware library.
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9.3.4
Equipping the ReservoirRack with reservoirs and ReservoirRack modules
Abb. 9-13: Equipping ReservoirRack
Fig. 9-13: Equipping ReservoirRack
Available tubs column
Save button
Reservoirs and equipped ReservoirRack modules
Save the changed combination.
Vendor Info field
Properties of the selected labware.
Save as button
Save the new combination.
Proceed as follows:
Prerequisites
• The ReservoirRack modules were created with the Module Rack + Tubes option.
• The labware is open.
1. Select the reservoir or equipped ReservoirRack module from the Available tubs column.
2. Drag the reservoir or equipped ReservoirRack module to the desired position in the ReservoirRack.
The epBlue worktable shows which reservoirs and ReservoirRack modules the ReservoirRack has been
equipped with.
3. To replace a ReservoirRack module, drag the new ReservoirRack module to the desired position.
The old ReservoirRack module is automatically removed.
4. Save the labware combination.
epBlue Labware Editor - Creating and managing labware
epBlue™ with MultiCon
English (EN)
9.4
Labware library
epBlue is delivered with a comprehensive labware library. The labware library contains data on the
geometry of reaction vessels, pipette tips, plates, racks, reservoirs, adapters and blocks. A complete
overview of the contents of the labware library from epBlue can be found on the www.eppendorf.com/
automation webpage.
Eppendorf AG creates labware definitions for labware from other manufacturers. Eppendorf AG does not
regularly check labware from other manufacturers. For example, if a manufacturer changes the dimensions
of a vessel, the labware definition no longer matches the changed vessel.
9.4.1
Loading labware definitions from the Internet
Eppendorf provides labware definitions on the www.eppendorf.com/automation webpage for download.
You can use different criteria to search on the webpage.
To download labware from the webpage, register in the VIP area on the www.eppendorf.com/automation
webpage.
To import the downloaded labware to epBlue (see p. 107).
9.4.2
Request labware definitions
Eppendorf creates labware definitions for labware that you would like to use in the epMotion. You can
record these definitions in the epBlue labware library.
1. Check to see if the definition for your labware is in the epBlue labware library.
2. Check to see if the definition for your labware is in the labware library on the www.eppendorf.com/
automation webpage.
3. If the definition is not available for your labware, send a request to Eppendorf AG.
If Eppendorf AG has a definition for your labware, you will receive the data from Eppendorf AG via
e-mail.
If Eppendorf AG does not have a definition of your labware, you will receive a form with questions on
your labware.
9.4.3
Add a labware definition to a labware library
 Import the labware (see Importing labware on p. 107).
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9.5
Special labware features in epBlue
9.5.1
Location B0 on the worktable
The dispensing tool cannot reach the location B0 on the worktable completely. The dispensing tool can only
fill the right locations of plates and ReservoirRacks.
 Use the location B0 for reservoirs.
 Use the location B0 to stack plates.
9.5.2
Tubes
Labware types in epBlue: Racks with Tubes > Tubes.
Reaction vessels are not available in epBlue as independent labware.
9.5.3
MTP plates
Labware types in epBlue: Plates > MTP.
Measuring the filling level is not available for MTP plates with 384 wells.
For MTP plates with 96 wells, measuring the filling level takes a long time.
9.5.4
PCR plates
Labware types in epBlue: Plates > PCR.
Labware
Labware types
PCR plates with 96 wells
PCR 96
PCR plates with 384 wells
PCR 384
9.5.5
Filter plates
Plates in the labware library can be found under Plates.
Labware
Labware types
Filter plates with 96 wells
Filter Plates 96
Filter plates with 384 wells
Filter Plates 384
epBlue Labware Editor - Creating and managing labware
epBlue™ with MultiCon
English (EN)
9.5.6
epT.I.P.S. Motion
Labware types in epBlue: Tips.
Pipette tips with filters have a "f" added to their name in epBlue.
Pipette tips
Example of names in epBlue
without filter
tip1000
with filter
tip1000f
Racks and Safe Racks have the same labware definition.
9.5.7
Racks
Labware types in epBlue: Racks with Tubes
Equip the racks with vessels before you use them in an application.
9.5.8
Rack 96
Labware types in epBlue: Racks with Tubes > Racks > Rack96_1_5 - 2_0
The labware definition of the Rack 96 only applies to the Rack. epBlue does not have a labware definition
for the Rack 96 and inserted reaction vessels.
 Create a labware definition from the labware definition of the Rack 96 and the labware definitions of the
used reaction vessels.
 Position the Rack 96 in row B and C, e.g. on locations B2 and C2.
epBlue detects that the Rack 96 occupies two locations on the worktable.
 Define the properties of the Rack 96 in the same way as the properties of other labware. E.g. you can
define the setting of the optical sensor.
9.5.9
Rack LC
Labware types in epBlue: Plates > Tube Plates 96
9.5.10
ReservoirRack
Labware types in epBlue: ReservoirRacks with Modules
Only irregular patterns may be set if the ReservoirRack is equipped with different ReservoirRack modules
and reservoirs.
Regular patterns may be set if the ReservoirRack is equipped with identical ReservoirRack modules or
identical reservoirs.
For each reservoir and each ReservoirRack module, the Detect volumes function of the optical sensor can be
activated.
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9.5.11
ReservoirRack Module TC
First, equip a ReservoirRack module with vessels. Then place the equipped ReservoirRack module in the
ReservoirRack.
The software only positions equipped ReservoirRack modules in a ReservoirRack.
Labware types in epBlue for unequipped ReservoirRack modules can be found in the folder: Racks with
Tubes > ThermoRacks + Modules.
Labware types in epBlue for reservoirs and equipped ReservoirRack modules can be found in the folder:
ReservoirRacks with Modules > Modules with Tubes.
Module
Labware type
ReservoirRack Module TC Ø 29 mm
Module TC 29mm
ReservoirRack Module TC Ø 17 mm
Module TC 17mm
ReservoirRack Module TC Reservoir 100 mL
Module TC Reserv100ml
ReservoirRack Module TC Reservoir 30 mL
Module TC Reserv30ml
ReservoirRack Module TC Ø 16 mm
Module TC 16mm
ReservoirRack Module TC Ø 12 mm
Module TC 12mm
ReservoirRack Module TC Safe Lock
Module TC Safe Lock
ReservoirRack Module TC PCR 0.5 mL
Module TC PCR 0_5ml
ReservoirRack Module TC PCR 0.2 mL
Module TC PCR 0_2ml
ReservoirRack module TC Eppendorf Tubes 5 mL
TC 5 ml module
9.5.12
Thermoracks
Labware types in epBlue: Racks with Tubes
The thermoracks must be equipped with vessels before they can be used in an application.
Thermoblock/thermorack PCR 96: This labware is defined as a thermoblock and a thermorack in the
labware library.
epBlue Labware Editor - Creating and managing labware
epBlue™ with MultiCon
English (EN)
9.5.13
Thermorack Rotor/Tubes
Order no.:
• Qiagen rotor disc 72: Order no.: 981301
• Qiagen rotor disc 100: Order no.: 981311
The tubes in the Qiagen rotor disc are very narrow.
With narrow tubes, the pipette tips can get jammed in the bottom section of the tubes. For this reason, the
Qiagen rotor disc has a higher bottom tolerance. Detailed information on the bottom tolerance can be found
in the labware definition.
 Only use the single-channel dispensing tool TS 50 when working with the Qiagen rotor disc.
You can only pipette into the narrow tubes of the Qiagen rotor disc with a single-channel dispensing tool
TS 50.
9.5.14
TipHolder
Labware types in epBlue: Adapter > Height.
The TipHolder is an adapter that holds the epT.I.P.S. Motion Reloads.
epT.I.P.S. Motion Reloads 1000 μL are positioned on the worktable using the TipHolder. To use epT.I.P.S.
Motion Reloads 50 μL and epT.I.P.S. Motion Reloads 300 μL with the TipHolder, program a height adapter
40 mm on the epBlue worktable. Pull the epT.I.P.S. Motion Reloads 50 μL or 300 μL onto the height
adapter.
9.5.15
Thermoadapter
Labware types in epBlue: Adapter > Thermo
In the software you can find the Thermoadapter LC Sample+Cartridge under Thermoblocks with plates.
The thermoadapter and plate combination is created during the editing of the worktable or via a transfer
command.
With the "Thermoadapter with skirted PCR plate" combination, the plate can be transported by the gripper.
The thermoadapter cannot be transported by the gripper.
For the Thermoadapter Frosty, select a skirted PCR plate for the location. The cooling aggregate is not
named in epBlue. In the software, the Thermoadapter Frosty can be found under Adapter > Height.
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9.5.16
Universal Disc by Focus Diagnostics
Order no.:
• Universal Disc: Order number at Focus Diagnostics: MOL1401
The epMotion fills the positions on the Universal Disc with sample material. The epMotion cannot extract
sample material from the positions on the Universal Disc.
 Work in the Pipette dispensing mode to add a second substance.
If you use the Multidispense dispensing mode, the liquid transfer is not performed in an optimum way.
9.5.17
Thermoblocks
Thermoblocks are heat-conductive adapters that can be transported with the gripper.
There are two types of thermoblocks available.
• Thermoblocks with plates are thermoblocks that are permanently connected with the plate.
• Thermoblocks are thermoblocks, into which the plates can be inserted.
Transport with the gripper
Thermoblock without plates
Thermoblock with plates
Thermoblock with plate
x
x
Thermoblock without plate
x
-
Insert plate in the thermoblock
x
-
Remove plate from the
thermoblock
x
-
9.5.18
Thermoblock PCR 96 OC
If you use the Thermoblock PCR 96 OC with a twin.tec PCR plate 96, semiskirted, you can use the TMX
template PCR 96 NGS. With the TMX template you can mix with a mixing speed of 1600 rpm for 2 minutes.
At a mixing speed of 1600 rpm, the maximum working volume is 140 μL. If the working volume is higher,
the liquid may be spilled.
The maximum mixing speed is 1700 rpm.
epBlue Application Simulator - simulating applications
epBlue™ with MultiCon
English (EN)
10
epBlue Application Simulator - simulating applications
This chapter describes how to simulate applications in epBlue.
10.1
Simulating the application
Before starting your application, you can simulate the run. When you start a simulation, no other simulation
can be open.
10.1.1
Starting a simulation
Prerequisites
• epBlue is started.
1. Press the Application Simulator symbol on the start screen.
2. Select the application in the Select Application area.
3. Press the Next button.
The application is opened. The connected epMotion is displayed in the Available Devices area.
4. Press the Next button.
5. Enter the number of samples for the current run.
6. Press the Next button.
7. Configure the functions of the optical sensor.
8. Press the Run button.
The simulation starts.
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10.1.2
Controlling the simulation
Abb. 10-1: Simulation window
Fig. 10-1: Simulation window
Prerequisites
• The simulation has started.
1. You can control the simulation with the following symbols:
Tab. 10-1: Symbols for controlling a simulation
Symbol
Meaning
Set the speed of the simulation.
You can set a speed from 1 – 10x. The figure 1 corresponds to real time. The figure 10
means 10 times faster than real time.
Start the simulation.
Perform a simulation step by step. The simulation stops after the next step.
Interrupt a simulation.
Cancel a simulation.
Exit the simulation. The simulation window is closed.
epBlue ID - Working with ID tracking
epBlue™ with MultiCon
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11
epBlue ID - Working with ID tracking
With epBlue ID, you document the way of samples and reagents during an application.
This chapter describes the basics of ID tracking. The chapter describes how to manage ID lists.
11.1
Scanning IDs
With epBlue ID, you can scan the IDs of samples and reagents.
IDs are linear codes or 2D codes. IDs are printed directly on the labware or on paper labels by the
manufacturer. IDs can be directly applied to the labware with a paper label.
IDs may contain further information, e.g., batch and expiry date of reagents. This information will be
included in the application log.
Information regarding which IDs are supported by epBlue can be found in the hardware manual for your
device.
You can use the following barcode readers for working with epBlue
• Barcode reader supplied with the epBlue ID software
Information on this barcode reader can be found in the hardware manual for your device.
• 2D barcode reader by Microscan
By default, epBlue uses the barcode reader supplied with the epBlue ID software. To install the 2D barcode
reader by Microscan, contact the authorized service of Eppendorf AG.
11.2
ID lists
11.2.1
Overview
The path of the samples is tracked and documented in the ID list during an application.
An ID list refers to a single labware. An ID list contains the IDs of the locations in the labware and
description of these locations. An ID list for plates also includes the ID of the labware.
epBlue creates ID lists during an application. If you abort an application, the ID lists receive the entries of
the processed locations.
ID lists are saved automatically. epBlue can import and export ID lists in the formats CSV and XML.
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Tab. 11-1: Contents of an ID list
Type of list
Input list
Result list
Table head, contains the labware ID in plates
Position
ID_1
ID_2
Position of a vessel in ID of the source
In input lists, this
the rack.
position.
field is empty.
The positions are
numbered by row.
Position on a plate,
e.g., A2.
Position of a vessel in ID of the destination epBlue adopts the
the rack.
position.
content of the ID_1
The positions are
field of the input list
numbered by row.
into the ID_2 field of
Position on a plate,
the result list.
e.g., A2.
The IDs are from the
positions the contents
of which have been
pipetted into this
position.
Name
Optional description
of the position.
epBlue adopts the
Name field from the
input list into the
result list. If the Name
field in the input list
is empty, epBlue
adopts the position
names of the source
labware into the
result list.
epBlue creates the Position, ID_1, ID_2, Name data fields for every used position in the labware.
11.2.2
Input lists and result lists
Input list
• ID list containing information on the source labware.
• The input list contains the IDs of the source positions.
• epBlue creates an input list for each source labware for which ID tracking is active.
• When using reagents with two IDs, e.g. for the type of the reagents and the batch number, one input list
contains two entries per position (see Making the basic settings for the epBlue ID on p. 131).
Result list
• ID list containing information on the destination labware and the intermediate labware.
• The result list contains the IDs of destination positions and intermediate positions.
• epBlue creates a result list for each destination labware and intermediate labware for which ID tracking
is active.
• If samples with different source positions are combined in a single destination position (pooled), the
result list contains multiple entries for the destination position. The source IDs are saved column by
column. For each transferred ID, two entries with identical content are created in the Pos and PosID_1
fields.
Tab. 11-2: ID lists and labware
Labware
Generated ID list
Rack with source vessels
Input list
Rack with source and destination vessels
Input list and Result list
Rack with destination vessels
Result list
Plate with source locations
Input list
Plate with source and destination positions
Input list and Result list
Plate with destination positions
Result list
ID lists for intermediate positions are created like ID lists for dstination positions.
epBlue ID - Working with ID tracking
epBlue™ with MultiCon
English (EN)
11.2.3
Reagents
Reagents are positions in the labware from which volume is removed only with the Reagent Transfer
command.
The following parameters for reagents can be defined in the epBlue ID basic settings:
• Number of the IDs for reagents (1 or 2)
• Do not display reagents in input lists and result lists. If labware ID tracking is activated, positions with
reagents are scanned anyway and documented in the log.
11.2.4
Samples
Samples are positions in the labware from which volume is not removed with the Reagent Transfer
command.
Samples have only one ID. Samples are always displayed in input lists and result lists (Tab. on p. 126).
11.3
Commands for working with IDs
If the application carries out the following commands, ID lists are created:
• Sample Transfer
• Reagent Transfer – optional
• Dilute
• Pool
• Pool one Destination
• Vacuum
For the command Reagent Transfer, it is possible to switch on and off the tracking of IDs as an option (see
Making the basic settings for the epBlue ID on p. 131).
If the Show reagent IDs in result list option is not activated, no ID tracking will take place. IDs are
documented in the log and in the input list. They are not transferred to the result list.
11.4
Reading IDs into epBlue
IDs can be entered when starting an application. To do so, enter the IDs manually or read them using the
barcode reader. Alternatively, you can use an ID list as an entry list.
Tab. 11-3: Differences when entering IDs
Labware
Entering the ID manually or using the
barcode reader
Entering the ID using the entry list
Plate
One ID per plate.
One ID per plate.
One ID per used well.
Rack
The rack does not have an ID.
The rack does not have an ID.
Rack with vessels
One ID per used vessel.
1 or 2 IDs if reagents are used.
One or several IDs per used vessel.
1 or 2 IDs if reagents are used.
Example: You are using a plate as destination labware. You have activated the Use Barcodes function in the
labware properties. When the application starts, you scan the ID of the plate manually. After the application
is complete, you receive a result list for the plate. The result list contains the ID of the plate and the IDs of
the used wells.
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11.5
Managing ID lists
Abb. 11-1: ID List Management window
Fig. 11-1: ID List Management window
Import symbol
Importing ID lists
User drop-down list
Filter ID lists according to user
Export symbol
Exporting ID lists
Type drop-down list
Filter ID lists according to input lists or result lists
Remove symbol
Deleting ID lists
Creation drop-down list
Filter ID lists according to type of creation
Date area
Filter ID lists according to date
11.5.1
Displaying ID lists
You can choose between two different views: List view and Application view.
1. Click on the ID List Management symbol on the start screen.
The ID List Management window opens.
2. Select the view via View in the menu bar.
epBlue ID - Working with ID tracking
epBlue™ with MultiCon
English (EN)
11.5.2
Filtering and sorting ID lists
You can filter ID lists by date, user, format or type of creation. You can filter ID lists by name, list ID and
date of creation.
Prerequisites
• The ID List Management window is open.
• The List view has been selected.
1. Select the criteria by which the ID lists are to be filtered. Click on the corresponding drop-down list and
select the criterion.
2. To sort the ID lists by name, list ID and date of creation click on the column title of the table.
3. To reset the sorting press the Clear button.
11.5.3
Importing ID lists
Users of the Admin Lab and User Level 2 user groups can import ID lists.
You can export ID lists in CSV or XML format.
Abb. 11-2: ID list in XML format
Fig. 11-2: ID list in XML format
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Abb. 11-3: ID list in CSV format
Fig. 11-3: ID list in CSV format
Prerequisites
• The ID List Management window is open.
1. Click on the
symbol in the menu bar.
The Select ID list window opens.
2. Select the ID list.
3. Press the Open button.
The Import ID list window appears. The window displays the path, format, type and content of the ID list.
4. Enter the new name for the ID list into the Name field.
5. Press the Import button.
The ID list appears in the ID List Management window.
11.5.4
Exporting ID lists
Users of the Admin Lab, User Level 2 and Administrator user groups can export ID lists.
Input lists and result lists can be exported in the formats CSV and XML. Exported ID lists can be saved to a
computer, network or LIMS. These ID lists can be used for other applications.
Prerequisites
• The ID List Management window is open.
1. Click on the
symbol in the menu bar.
2. Select the ID list.
3. Press the Export ID lists button.
4. Select the storage location for the ID list.
5. Press the Save button.
11.5.5
Deleting ID lists
Users of the Admin Lab user group can delete ID lists.
Prerequisites
• The ID List Management window is open.
 Select the ID list.
 Press the
symbol.
The ID list is deleted.
epBlue ID - Working with ID tracking
epBlue™ with MultiCon
English (EN)
11.6
Making the basic settings for the epBlue ID
In order to make the basic settings for the epBlue ID, you must be part of the Admin Lab user group.
Abb. 11-4: ID List Settings window
Fig. 11-4: ID List Settings window
Show reagent IDs in result list
Import/Export default directory
Switch on and off the ID tracking for the Reagent
Define the default folder for the import and
Transfer command.
export of ID lists.
Maximum number of reagent IDs
ID list import with checksum validation
Set the maximum number of IDs to be processed
Switch on and off the checksum validation when
with the Reagent Transfer command.
importing ID lists.
Import/Export default file type
Define the file format for the import and export of
ID lists.
11.6.1
Making the basic settings for the epBlue ID
Prerequisites
• epBlue is started.
• A user from the Admin Lab user group is logged in.
1. Click on the Settings symbol on the start screen.
2. Touch and hold on the Barcode tab in the Overview window.
The General barcode settings window appears.
3. Select Settings.
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epBlue settings - Checking components and making the basic settings
epBlue™ with MultiCon
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12
epBlue settings - Checking components and making the basic settings
This chapter describes how to check components of the epMotion and make the basic settings.
Prerequisites
• epMotion is ready for operation.
• epBlue is started.
• The start screen will be displayed.
1. Press on the Settings symbol on the start screen.
2. Select the function.
12.1
Overview
Which tabs are displayed depends on your user group.
12.2
Levelsensor options
The Levelsensor options function is used to define the functions the optical sensor will execute. This setting
is used as the default.
The time for the application is reduced when the optical sensor is switched off. The volume must be entered
when the application is started.
Abb. 12-1: Levelsensor options function
Fig. 12-1: Levelsensor options function
Detect volumes radio
button
The optical sensor measures the filling volume. It is determined in the
properties of the labware, in which positions the optical sensor measures the
filling volume.
Use required minimum
volumes radio button
epBlue assumes that all vessels are filled with the minimum required volume for
this run.
Input volumes manually
radio button
Enter filling volume manually.
Slider Detect tips
The optical sensor checks, in which positions of the labware pipette tips are
available.
Check labware
placement slider
The optical sensor checks if labware is positioned on the worktable.
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Information on the optical sensor (see Optical sensor on p. 93).
12.3
Tool interlock
The Tool interlock function can be used to check the locking mechanism of the dispensing tools and
gripper.
Abb. 12-2: Tool interlock function
Fig. 12-2: Tool interlock function
1 Zero value sensor field
0 = Dispensing tool is not in home position. 1 =
Dispensing tool is in home position.
5 Refresh button
Update fields 1 – 4
6 Lock button
2 Tool detection sensor field
Lock the dispensing tool
0 = Dispensing tool not available. 1 = Dispensing
7 Unlock button
tool available.
Unlock the dispensing tool
3 Interlock field
OPEN = Locking mechanism opened.
CLOSED = Locking mechanism closed.
4 Current Tool field
Name of the tool used
12.3.1
Lock the tool
1. Slide the tool into the tool holder from below.
2. Hold the tool tightly At the same time, press the Lock button.
The tool holder locks the tool.
12.3.2
Unlocking the tool
1. Hold the tool tightly At the same time, press the Unlock button.
The tool holder unlocks the tool.
2. Remove the tool.
epBlue settings - Checking components and making the basic settings
epBlue™ with MultiCon
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12.4
Dosing device
The Dosing device function can be used to read out the number of stroke movements of a dispensing tool.
Abb. 12-3: Dispensing Device function
Fig. 12-3: Dispensing Device function
Tool used
Name of the tool
Tool path
Path of the labware definition of the tool
12.4.1
Strokes
Number of stroke movements of the dispensing
tool
Reading out the tool
Prerequisites
A tool is in the tool holder.
 To read out the tool data, click on the Read button.
12.5
Gripper
The Gripper function can be used to open and close the gripper. Labware can be removed from the gripper
if necessary.
Abb. 12-4: Gripper function
Fig. 12-4: Gripper function
Distance field
Distance of gripper arms
Set button
Confirm new distance of gripper arms
Gripper distance (µm) field
Enter new distance of gripper arms
Export field
Exports data
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12.5.1
Moving the gripper arms
Prerequisites
• The gripper is located in the tool holder.
1. To move the gripper arms, enter a value in the Gripper distance field. The value must be approx. 20%
larger than the value in the Distance field.
2. Confirm your entry with the Set button.
The gripper can now be moved.
12.6
Temperature control unit X
The Temperature control unit X function enables you to check the thermal modules of the epMotion.
Abb. 12-5: Temperature control unit function
Fig. 12-5: Temperature control unit function
Desired Temperature
Shows the set temperature of the thermal
module.
Remain time
Shows the time remaining until the end of
temperature control (in min).
Actual Temperature
Shows the set temperature of the thermal
module.
Temp reached after
Shows the time required to reach the set
temperature.
Actual current
Shows the current strength in the thermal
module.
New Temperature
Set temperature (–5°C to 110°C)
Time
Shows the temperature control duration in min.
The current status is shown in the State area.
New time
Temperature control duration (0 to 99 min)
epBlue settings - Checking components and making the basic settings
epBlue™ with MultiCon
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You can make settings in the Set Temperature area.
1. Enter the set temperature in the New Temperature field.
2. Enter the test duration in the New time field.
3. Click on the Start button. The thermal module heats or cools until the set temperature has been reached
or the set time has elapsed. If the set temperature is reached before the set time has elapsed, the
thermal module maintains the set temperature until the end of the test.
4. To update the display during the test, press the Refresh button.
5. To activate the fan, press the Fan On button.
12.7
ThermoMixer
This function enables you to check the Eppendorf ThermoMixer module.
NOTICE! Contamination of samples and the device due to excessive speeds.
If the speed is set too high, liquid sprays out of the vessels. Labware may become loose from
the holder and fly around.
 Observe the labware maximum speeds.
Tab. 12-1: Maximum speed for labware on the Eppendorf ThermoMixer module
Labware
Maximum speed in rpm
Vessels
1000
Deepwell plates with 96 wells
1200
Deepwell plates with 384 wells
1200
Microplate with 6 wells
1000
Microplates with 96 wells
2000
Microplates with 384 wells
2000
PCR plate with 96 wells
2000
PCR plate with 384 wells
2000
ReservoirRack
Mixing not possible
Thermorack or Thermoblock
1000
Thermoblock 96 with a semi-skirted PCR plate for 250 μL
1700
Thermoadapter for Microplate 96/U/V with Eppendorf Microplate 96/V
1600
Thermoadapter for Microplate 96/U/V with Eppendorf Microplate 96/U
1600
Thermoblock PCR 96 OC
1700
Thermoadapter for 96 wells
1000
Thermoadapter for 384 wells
1000
Thermorack TMX
1300
PrepRack
1300
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12.7.1
Checking the mixing function of the Eppendorf ThermoMixer module
Abb. 12-6: Mixer Control Thermomixer function
Fig. 12-6: Mixer Control Thermomixer function
RPM field
Remaining time field
Speed of the Eppendorf ThermoMixer modules in
Remaining time in the mixing process.
rpm.
Start button
Time field
Stop button
Duration of the mixing process.
1. Define the speed and temperature in the Parameter area.
2. To check the mixing function, press the Start button.
The Eppendorf ThermoMixer module mixes until the time has elapsed or until the test has been exited
using the Stop button.
12.7.2
Checking the temperature control of the Eppendorf ThermoMixer module
Abb. 12-7: Thermomixer temperature control function
Fig. 12-7: Thermomixer temperature control function
Desired Temperature field
Set temperature of the Eppendorf ThermoMixer
module.
Actual Temperature field
Actual temperature of the Eppendorf ThermoMixer
module.
Actual current field
Current strength via the thermal module.
Time field
Mixing time in min.
Remain time field
Time until the end of the mixing process (in min).
Temp reached after field
Time until the set temperature is reached.
New Temperature field
Enter a new set temperature. 4 °C– 95 °C
range.
New time field
Enter a new mixing time. 0 s - 99 min range.
Start button
Start the test.
Fan on button
Switch on the fan.
epBlue settings - Checking components and making the basic settings
epBlue™ with MultiCon
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The current status is shown in the State area.
You can make settings in the Set Temperature area.
1. Enter the set temperature in the New Temperature field.
2. Enter the mixing time in the New time field.
3. Press the Start button.
The Eppendorf ThermoMixer module tempers the labware to the set temperature. If the temperature is
reached before the mixing time has elapsed, the Eppendorf ThermoMixer module maintains a constant
temperature until the end of the test.
12.8
Magnetic separation
The Magnetic Separation function can be used to check the magnetic finger module.
Abb. 12-8: Magnetic Separation function
Fig. 12-8: Magnetic Separation function
Home
Move the magnetic finger module to the home
position.
Draw Out
Draw out the magnetic fingers.
Draw In
Draw in the magnetic fingers.
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12.9
UV lamp
The UV lamp is used to decontaminate the epMotion worktable. An irradiation cycle lasts 15 min.
12.9.1
Decontaminating the worktable
CAUTION! Risk to health due to UV radiation.
UV radiation causes eye and skin damage.
UV radiation may escape outward through the ventilation slits in the rear panel of the device.
 Wear your personal protective equipment when you are near the ventilation slits.
NOTICE! Damage to plastic due to UV radiation.
Plastics become brittle as a result of UV radiation exposure.
 Remove the dispensing tools, labware, and waste container from the worktable before
switching on the UV lamp.
Prerequisites
•
•
•
•
•
The epMotion is equipped with a CleanCap.
The dispensing tools, labware and waste container have been removed from the worktable.
The front hood is closed.
The epMotion is ready for operation.
epBlue has been started.
1. Select the Settings symbol on the start screen.
2. On the epMotion serial number tab, select the UV lamp function.
3. Click on the UV Light OFF button.
The UV Lamp window appears.
4. Check to see if the worktable is empty and the front hood is closed.
5. Click on the OK button to start the disinfection.
epBlue settings - Checking components and making the basic settings
epBlue™ with MultiCon
English (EN)
Abb. 12-10: UV Light button
Abb. 12-9: Lamp symbol in status area
Fig. 12-9: Lamp symbol in status area
Fig. 12-10: UV Light button
The tool holder moves. The UV lamp will be switched on.
The UV Light button shows the remaining irradiation period. The lamp symbol appears in the status area.
12.10
HEPA air filter
Air is drawn in using a fan. The air drawn in is filtered using the HEPA air filter. A continuous and filtered
air flow is guided into the epMotion workspace. The air flow prevents the workspace from being
contaminated with outside air.
12.10.1 Switching on the HEPA air filter
WARNING! Risk of infection due to aerosols.
If you work with the HEPA filter, a continuous air flow is blown from the device workspace to
the environment. The air flow can transport aerosols and contaminate the ambient air.
 Do not use the HEPA filter when working with biohazardous substances.
 Disinfect contaminated surfaces in the workspace before switching on the HEPA filter.
Prerequisites
•
•
•
•
•
The epMotion is equipped with a CleanCap.
All surfaces in the workspace are disinfected.
The front hood is closed.
The epMotion is ready for operation.
epBlue has been started.
 Select the Settings symbol on the start screen.
 On the epMotion serial number tab, select the HEPA filter function.
 Press the HEPA filter ON button.
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Abb. 12-11: Filter symbol in the status area
Fig. 12-11: Filter symbol in the status area
The HEPA air filter is switched on. The filter symbol appears in the status area.
The HEPA air filter remains switched on if you exit the HEPA filter function. Now start your application.
12.10.2 Switching off the HEPA air filter
Prerequisites
• The HEPA air filter is running.
• The HEPA filter function on the epMotion serial number > Settings tab was selected.
1. Press the HEPA filter ON button.
The HEPA air filter is switched off.
2. Alternatively, you can switch off the HEPA air filter when you switch off the epMotion using the mains/
power switch.
12.11
Setting the barcode reader
The following settings are available for the barcode reader:
• Manual/Serial: Reading in barcodes by confirming the button on the barcode reader
• Presentation Mode: The barcode reader automatically scans for barcodes
Loading, saving or resetting the settings
• Load: checks the setting of the barcode reader
• Save: saves the setting of the barcode reader
• Set default: resets the barcode reader to factory settings
epBlue settings - Creating and managing users
epBlue™ with MultiCon
English (EN)
13
epBlue settings - Creating and managing users
This chapter describes how you can create and manage users as an administrator. The chapter describes
how to change your password as a user.
13.1
Managing the user accounts as an administrator
13.1.1
Logging in as administrator in epBlue
Prerequisites
• epBlue is started.
• epBlue displays the login screen.
1. Enter the user name Administrator.
2. Enter the admin password Admin123!.
3. Press the Login button.
You are logged in as administrator.
Change the administrator password after the first login. This is the only way to prevent
unauthorized access to your data. To change the password (see Changing the password of your
own user account on p. 151).
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13.1.2
epBlue user groups
The Account management > Group overview tab displays an overview of the user groups. Each user group
has defined user rights. Anyone who has a user account must be assigned to a user group.
Abb. 13-1: Overview of the user groups and their user rights.
Fig. 13-1: Overview of the user groups and their user rights.
Group column
Available user groups.
Fixed checkbox
The checkbox is activated. The user rights of a
group cannot be changed.
System, Application, Labware … columns
Groups of user rights. Active rights are marked
with an X for each user group.
Administrator
• Importing, exporting, and printing data.
• Saving and recovering data.
• Using the customer service functions.
• Configuring epBlue.
• Managing user accounts.
• Setting up epMotion and epBlue.
Administrator Lab
• Creating, editing, and starting applications.
• Making settings for ID.
• Importing, exporting, and printing data.
• Activating, deactivating, creating, and editing labware.
• Using the customer service functions for applications.
epBlue settings - Creating and managing users
epBlue™ with MultiCon
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User level 2
• Creating, editing, and starting applications.
• Importing, exporting, and printing data.
• Activating, deactivating, creating, and editing labware.
User level 1
• Creating, editing, and starting applications.
• Printing data.
Guest
• Starting applications
System
• Self-management of the system.
• Seeing applications.
• Seeing labware.
13.1.3 User accounts
13.1.3.1 Overview
The Account > Overview tab displays a list of all user accounts for epBlue. Here you can see which users
have user accounts in epBlue.
Abb. 13-2: Overview of user accounts
Fig. 13-2: Overview of user accounts
Overview button
Administrate button
Name of the user, company, e-mail address, and
User directory, registration date, user group,
phone number
expiration date for using the software
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13.1.3.2 Creating a user account
Create a user account for each user. Each user name can only be used once. Deleted user names cannot be
used again.
Abb. 13-3: Creating a user account
Fig. 13-3: Creating a user account
Prerequisites
• epBlue is started.
• You are logged in as administrator.
• The Settings area is open.
1. Select the Account management > Create account tab.
The Create Account window appears.
2. Enter the user name.
3. Enter the password into the Password and Confirm password fields.
If the content of the Password and Confirm password differs, an error message appears.
4. Enter the expiry date of the account into the Valid until field.
5. Enter the date until which the account is to be disabled into the Disabled until field.
6. Activate the radio button for the user group in the Group area.
7. Press the Create Account button.
The user account is created. The user name appears on the Account management tab. In the Application
Editor a folder is created for the user.
epBlue settings - Creating and managing users
epBlue™ with MultiCon
English (EN)
13.1.3.3 Editing a user account
Abb. 13-4: Account management tab
Fig. 13-4: Account management tab
Prerequisites
• epBlue is started.
• You are logged in as administrator.
• The Settings area is open.
1. Select the Account management tab.
2. Select users in the Users column.
The user account opens.
3. Press the Overview button. Change the entries in the fields.
4. Press the Administrate button. Change the entries in the fields.
5. Press the Submit button.
The changes are saved.
13.1.3.4 Deleting a user account
Prerequisites
• epBlue is started.
• You are logged in as administrator.
• The Settings area is open.
1. Select the Account management tab.
2. Select users in the Users column.
The user account opens.
3. Press the Administrate button.
4. Press the Delete button.
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5. epBlue asks whether you would like to delete the user account.
To delete the user account, click on Yes .
If you do not want to delete the user account, click No.
13.1.4
Assigning a new password for a user account
If the user has forgotten his password, the administrator can assign a new password.
Prerequisites
• epBlue is started.
• You are logged in as administrator.
• The Settings area is open.
1. Select the Account management tab.
2. Select users in the Users column.
The user account opens.
3. Press the Administrate button.
4. Press the Set a new password button.
A window appears.
5. Enter the new password into the New Password and Confirm password fields.
If the entries differ, an error message appears.
6. Press the Submit button.
The new password is now active.
13.1.5
Creating and validating checksums
Checksums are used to validate files that are imported to epBlue. This ensures errors are detected when
data is transferred or if data is manipulated. You can validate the checksum of files when importing to
epBlue or in external software.
epBlue settings - Creating and managing users
epBlue™ with MultiCon
English (EN)
13.1.5.1 Importing and validating files with checksums
epBlue cannot check the checksums of files if the files are in zip, dws, lhs, or was format.
epBlue cannot check the checksums of files stemming from older versions of epBlue or from
the epMotion 5075 control panel.
If you import the following data, epBlue checks the checksums.
File format
Application
Checksum
.export
• Applications
• Labware
• The checksum is always checked.
.csv
• Applications
• ID lists
.xml
• ID lists
• If checking of the checksum was activated, epBlue checks
the checksum.
• If checking of the checksum was not activated, epBlue
ignores any existing checksums.
Prerequisites
• Checking of the checksum is activated.
 Files are imported as described in the software operating manual.
If the check of the checksum was successful, the file is imported.
If the checksum is invalid or no checksum is present, a warning message appears.
13.1.5.2 Exporting and validating files with checksums
Tab. 13-1: Files that have a checksum after the export from epBlue
File format
File
.export
• Application
• Labware
.csv
• ID lists
.xml
• ID lists
 Files are exported as described in the software operating manual.
13.1.5.3 Validating checksums in external software
To validate a checksum from epBlue, proceed as follows:
1. Save the checksum.
2. In the imported file, delete the line containing the checksum.
3. Delete line breaks in the file.
4. Calculate the checksum for the file using the SHA256 algorithm.
The algorithm is included in the Microsoft .NET Framework.
5. Compare the calculated checksum with the saved checksum.
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13.1.5.4 Creating checksums in external software
For epBlue to be able to validate the checksums during import, the checksums have to be created as
follows.
1. To calculate the checksum, remove the line breaks.
2. Calculate the checksum using the SHA256 algorithm.
This algorithm is included in the Microsoft .NET Framework.
3. Enter the checksum in the file.
Tab. 13-2: Checksums for certain file formats
CSV file
In line 5, column 1 = checksum, column 2 = calculated checksum
XML file
In <Checksum> tag under <ID list>
Abb. 13-5: ID list in csv format with checksum
Fig. 13-5: ID list in csv format with checksum
Abb. 13-6: ID list in xml format with checksum
Fig. 13-6: ID list in xml format with checksum
epBlue settings - Creating and managing users
epBlue™ with MultiCon
English (EN)
13.2
Managing your own user account
13.2.1
Signing into and out of the user account
 How to sign in and out of your user account (see Logging in as user on p. 19) and (see Logging off as a
user on p. 20).
13.2.2
Changing the password of your own user account
Users of user level 2 or higher can change their own password themselves. The administrator can change
the passwords of all users.
Prerequisites
• epBlue is started.
• You are in signed in to your user account.
• The Settings area is open.
1. Select the Account Management tab.
2. Select Edit own account.
The user account opens.
3. Press the Change Password button.
Abb. 13-7: Change Password
Fig. 13-7: Change Password
The Account window appears.
4. In the Old Password field, enter the current password.
5. Enter the new password into the New Password and Confirm new password fields.
If the entries differ, an error message appears.
6. Press the Submit button.
The new password is now active.
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13.2.3
Activating warning messages
Abb. 13-8: Warning message on adjusting the epBlue worktable
Fig. 13-8: Warning message on adjusting the epBlue worktable
Users of user level 2 or higher can define whether warning messages should be displayed in epBlue (see
Inserting commands from another application on p. 51). Warning messages are displayed in the basic
setting.
Abb. 13-9: User defined settings tab
Fig. 13-9: User defined settings tab
Prerequisites
• epBlue is started.
• You are in signed in to your user account.
• The Settings area is open.
1. Select the Account management tab.
2. Select User defined settings.
3. Set the slider to Yes or No.
4. Press the Apply button.
The setting will be saved.
epBlue Settings - Saving data and creating print templates
epBlue™ with MultiCon
English (EN)
14
epBlue Settings - Saving data and creating print templates
This chapter describes how to backup the database, create print templates and change the name of your
device.
14.1
Data backup
epBlue saves applications, protocols, the labware library and information on user accounts in a database.
The Backup function can be used to create a backup copy of the database. The backup copy can be used to
restore the database after a system failure.
You can save data and load a backup copy if you are logged in as administrator.
NOTICE! Data loss due to missing data backup or incorrect storage of data carriers.
User accounts, applications, labware and logs are stored in one database. If this database gets
damaged (e.g. due to a hardware defect), the information is lost.
Eppendorf is not liable for data loss of data. Eppendorf is not liable for damage arising as a
result of data loss.
 Backup the database regularly. Only use the epBlue software backup.
 Save stored data on external data carriers.
 Store the storage media according to the manufacturer's specifications.
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14.1.1
Backing up the database
Abb. 14-1: Backup window
Fig. 14-1: Backup window
Destination selection area
Location where a backup copy will be saved.
Details field
Information on backup
Status field
Shows if the software is ready or damaged.
Backup button
Starts the data backup.
Progress bar
Progress of backup
Proceed as follows:
Prerequisites
• epBlue is started.
• You are logged in as administrator.
1. Press the Settings symbol on the start screen.
2. Select the Backup and Restore tab.
3. Select Backup.
4. Press the
button.
5. Select the folder the backup will be saved to.
A name for the backup is automatically assigned.
6. Use that name for the backup or select a new name.
7. Start the backup using the Backup button.
epBlue Settings - Saving data and creating print templates
epBlue™ with MultiCon
English (EN)
14.1.2
Creating an automatic backup
Proceed as follows:
Prerequisites
• epBlue is started.
• You are logged in as administrator.
1. Press the Settings symbol on the start screen.
2. Select the Backup and Restore tab.
3. Select Automatic Backup.
4. Select the Create full Backup every checkbox.
5. Enter the time in days after which an automatic backup is performed.
6. Press the
button.
7. Select the folder the automatic backup will be saved to.
8. To reset the entries, press the Reset button.
9. To start the backup, press the Apply button.
14.1.3
Restoring the database
You can import your backup copy in epBlue. The database will be restored to the status at the time of the
backup.
All data in epBlue is overwritten when you import the backup copy. Changes made after this
backup copy was created will be lost.
Abb. 14-2: Restore window
Fig. 14-2: Restore window
Source Full Backup
Location where a backup copy is saved.
Status field
Shows if the software is ready or damaged.
Progress bar
Progress of backup
Details field
Start button
Imports the backup copy in epBlue.
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Prerequisites
• epBlue is started.
• You are logged in as administrator.
1. Connect the USB storage medium to the control device.
2. Press the Settings symbol on the start screen.
3. Select the Backup and Restore tab.
4. Select Restore.
5. Press the
button.
6. Select a backup copy with the *.bak format.
7. To import the backup copy into epBlue, click on the Restore button.
14.1.4
Importing a database from epBlue version 40.X
You can use this function to import databases from the epBlue versions 40.X into epBlue from version 40.4.
Abb. 14-3: Migrate 40.x database window
Fig. 14-3: Migrate 40.x database window
Prerequisites
• epBlue is started.
• You are logged in as administrator.
1. Press the Settings symbol on the start screen.
2. Select the Backup and Restore tab.
3. Select Migrate 40.x database.
4. Press the
button.
5. Select a backup copy with the bak format.
6. To import the database into epBlue, press the Migrate button.
epBlue Settings - Saving data and creating print templates
epBlue™ with MultiCon
English (EN)
14.2
Changing the device name
In the Devices window you will find information on the status and the configuration of the epMotion. The
Devices > Settings > Detail information > Nickname shows the name of the epMotion. The serial number will
be assigned as the name by default. You can change the name of the device.
You can check the settings if you are logged in as an administrator.
Abb. 14-4: Device name window
Fig. 14-4: Device name window
Prerequisites
• You are logged in as administrator.
• epBlue is started.
1. Select the Settings symbol on the start screen.
2. On the Devices tab, select the Settings function.
The Detail Information window will appear.
3. Change the device name in the Nickname field.
4. To save the changes, press the Save button.
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14.3
Print templates
Print templates can define how your print-outs and PDF documents look.
Abb. 14-5: Print Header window
Fig. 14-5: Print Header window
Current preview setting drop-down list
Select a print template.
Show preview button
Show a preview.
Add button
Create a print template.
... button
Import a graphic for the header.
Edit button
Change a print template.
Save button
Save a print template.
Remove button
Delete a print template.
Cancel button
Cancel the process.
Save as default button
Save print template as default.
epBlue Settings - Saving data and creating print templates
epBlue™ with MultiCon
English (EN)
14.3.1
Creating a print template
Prerequisites
• You are logged in as Administrator or Admin Lab.
• epBlue is started.
1. Press the Settings symbol on the start screen.
2. Select the Print Header function.
The Print Header window appears.
3. Click on the Edit print header template button.
4. Enter your company's address in the Company Information field.
5. Import a graphic for the header. by clicking on the
button.
epBlue only imports graphics in the formats GIF, JPEG, and PNG.
6. To show a preview, click on the Show preview button.
7. To save the template, click on the Save button.
8. Enter the name of the template and confirm it using the OK button.
The template will be saved.
14.3.2
Saving print templates as default
Prerequisites
• You are logged in as Administrator or Admin Lab.
• epBlue is started.
1. Press the Settings symbol on the start screen.
2. Select the Print Header function.
The Print Header window appears.
3. Select the print template in the Current preview settings menu.
4. Click on the Set as default header button.
The print template is saved as standard.
14.3.3
Deleting a print template
Prerequisites
• You are logged in as Administrator or Admin Lab.
• epBlue is started.
1. Press the Settings symbol on the start screen.
2. Select the Print Header function.
The Print Header window appears.
3. Select the print template in the Current preview settings menu.
4. Press the Remove button.
The print template is deleted.
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epBlue System log viewer - managing database logs
epBlue™ with MultiCon
English (EN)
15
epBlue System log viewer - managing database logs
This chapter describes how to view and print database logs.
15.1
Audit trail
Abb. 15-1: Audit trail
Fig. 15-1: Audit trail
The time stamp displays the time according to the time zone set in the operating system
configuration. If the time zone is changed, the time stamps will be automatically adjusted. The
set time zone is included in the audit trail printout.
15.1.1
Opening the audit trail
 In epBlue, open the System log viewer area.
The audit trail is displayed as a table. Each line shows an entry.
15.1.2
Displaying the details of an entry
 Select the entry in the table.
The details appear in the Selected entry area.
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15.1.3
Sorting entries
1. Click the column header by which sorting should be performed.
The table is sorted in ascending order.
2. Click the column header again to sort in descending order.
15.1.4
Creating a filter
1. Click the New Filter symbol.
The Filter window appears.
2. Specify the filter name.
3. Define the filter criteria.
4. Use the Add button to add more filter criteria.
5. Press the Save button to save the filter.
15.1.5
Editing a filter
1. Click the Edit Filter symbol.
The Filter window appears.
2. Change the filter name, if relevant.
3. Change the filter criteria.
Use the Add button to add filter criteria.
Use the Remove button to delete filter criteria
4. Press the Save button to save the filter.
15.1.6
Selecting a filter
 Select the filter in the drop-down menu of the menu bar.
The audit trail is adapted to the filter.
15.1.7
Deleting a filter
1. Select the filter in the drop-down menu of the menu bar.
The audit trail is adapted to the filter.
2. Press the Trash symbol to delete the opened filter.
15.1.8
Selecting columns to be displayed
 Open the Select Visible Columns drop-down menu.
 Select the columns to be displayed.
epBlue System log viewer - managing database logs
epBlue™ with MultiCon
English (EN)
15.1.9
Printing and exporting the audit trail
You can print the displayed part of the audit trail or export it as a PDF document.
1. Access the audit trail.
2. Select a filter, if relevant.
3. Press the
symbol.
The print preview opens.
4. Click the
5. Press the
symbol in the print preview to print the audit trail.
button in the print preview to export the audit trail as a PDF document.
15.1.10 Audit trail categories
The audit trail saves events as entries. Each event that receives an entry is assigned to a category.
Tab. 15-1: Changing user data
Category
Event
New Application
Application Changed
Application Deleted
Application Saved Under
New Name
Application Imported
Application Exported
Application Printed
• The user has created a new application.
• The user has changed or deleted an existing unsigned application or
saved it under a new name.
• The user has imported or exported an application.
• The user has printed an application or exported it as a PDF document.
Old Application Updated
• In the Application Editor the user has confirmed the action of updating an
imported application that was created with an older software version.
Official Application
Changed
Official Application Copied
• The user has changed a signed application and thus created a new
unsigned revision.
• The user has copied a signed application.
Application Signed
Application Authorized
• The user has signed an application as created or reviewed.
• The user has signed an application as authorized.
Application Log Printed
• The user has printed a log or exported it as a PDF document.
Labware Created
Labware Changed
Labware Deleted
Labware Copied
Labware Imported
Labware Exported
Labware Printed
•
•
•
•
The user has created labware.
The user has changed, deleted, or copied existing labware.
The user has imported or exported labware.
The user has printed labware or exported it as a PDF document.
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Category
Event
ID List Created
ID List Exported
ID List Removed
epBlue ID
• The user has created an ID list. The ID list was created when importing a
file or when executing an application with ID tracking.
• The user has exported an ID list.
• The user has removed an ID list.
Archive
• A user belonging to the user group Administrator has archived one or
more applications or parts of the audit trail.
Tab. 15-2: Executing applications
Category
Event
Application Started
Application Ended
• The user has started an application.
• An application has been ended.
Number of Samples
• The user has manually entered the number of samples for an application.
Application Stopped
Single-Step Application
Continue Application
• The user has stopped an application.
• The user has executed a step in the stopped run.
• An application has been continued.
Application Error
• An error has occurred during an application.
Application Aborted
• An application has been aborted.
Tab. 15-3: User accounts and user actions
Category
Event
Login
Logout
• A user has logged into epBlue GxP or logged out.
Idle Session Blocked
Idle Session Unblocked
• epBlue GxP has been locked. The user has not made any entries for a
defined period.
• A user has unlocked epBlue GxP by entering his password.
User Added
User Deleted
User Group Changed
Password Changed
• A user belonging to the user group Administrator has created a new user
account or deleted a user account.
• A user belonging to the user group Administrator has assigned a user
account to another user group.
• A user has changed his password or a user belonging to the user group
Administrator has set a new password for a user.
False Login User Disabled
• The user has repeatedly entered an incorrect password. The user account
has been locked.
Disabled User Reactivated
• A user belonging to the user group Administrator has unlocked a locked
user account.
Password Expired
• The validity of a password has expired.
Account Expired
• The validity of a user account has expired.
epBlue System log viewer - managing database logs
epBlue™ with MultiCon
English (EN)
Tab. 15-4: Setting and changing the system
Category
Event
Device Found
Device Added
Device Lost
• epBlue has detected a connected device.
• epBlue has detected an epMotion and added it to the selection.
• The communication between epBlue and an epMotion has been
interrupted.
Device Initialization Failed
• epBlue was unable to initialize a connected device.
Device Status Changed
• The status of the device has changed, for example, an application has
been started or stopped.
Device Firmware Update
• A user belonging to the user group Administrator Lab or Service has
installed a different firmware version of epMotion.
Barcode Settings Changed
epBlue ID
• A user belonging to the user group Administrator Lab has changed the
barcode reader settings.
Number of Reagent IDs
Changed
Import/Export Settings for
ID List Changed
epBlueID
• A user belonging to the user group Administrator Lab has changed the
number of required IDs or barcodes for reagents.
• A user belonging to the user group Administrator Lab has changed the file
format or the storage location for the import or export of ID lists.
Checksum Settings
Changed
epBlueID
• A user belonging to the user group Administrator or Administrator Lab has
changed the setting for the checksum validation of ID lists or CSV files.
Number of Authorization
Signatures
• A user belonging to the user group Administrator has changed the
authorization scheme of applications or logs.
Password Lifetime Changed
Maximum Login Retries
Changed
Max Idle Time Changed
• A user belonging to the user group Administrator has changed the
duration of the validity of user account passwords.
• A user belonging to the user group Administrator has changed the
number of failed logins after which a user account is locked.
• A user belonging to the user group Administrator has changed the time
after which a user account is locked when the user has not performed any
actions.
Auto Backup
Database Successfully
Restored
• epBlue has performed an automatic backup. An error has occurred while
implementing the automatic backup.
• A user belonging to the user group Administrator has restored the epBlue
database from a backup.
Dialog Message
• epBlue has displayed a message.
epBlue Application Error
• An error has occurred while creating a digital signature.
• A software error has occurred.
Device Configuration
Changed
Service Function
• Authorized service personnel have changed the configuration of the
device.
• Authorized service personnel have performed a test function or changed a
setting of the device.
165
166
epBlue System log viewer - managing database logs
epBlue™ with MultiCon
English (EN)
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
16
epBlue - Software error messages
This chapter contains an overview of the software error messages, causes and solutions.
16.1
Error messages
If an error occurs, first try the following:
1. Switch off epMotion.
2. Restart the epMotion.
3. Execute the application again.
If the error occurs again, contact Eppendorf Service or your dealer. The addresses can be found on our
webpage www.eppendorf.com.
Code
Problem
Message without Make sure that there is
code
nothing in the gripper: If
any labware is held by
the gripper arms remove
this item manually; then
press Ok.
(without code)
Solution
Method run was aborted;
If the gripper is still holding
gripper is still fixed in the tool any items:
holder; before tool holder
1. Remove the item manually
moves further (e.g., to its home
by opening the two
position) the message appears.
gripper arms manually
and removing the labware
manually.
2. Press the Ok button.
The gripper expects that the
labware has been removed
and now moves to the home
position.
Because all tool holder Method run was aborted;
positions are occupied, Dispensing tool is still fixed in
the tool has to be
the tool holder.
removed manually:
Press Ok, open the hood
and be prepared to grasp
the tool manually as the
tool holder opens and
drops the tool.
0x0600, 0x060E Tool did not find home
0x0C01
Cause
• Home position for the tool is
not found.
• No tool inserted.
• Tool damaged.
Volume too large for this Volume to be dispensed is too
tool
large for the selected tool.
Possible causes:
• Errors in tool files.
• Errors in liquid type files.
 To remove the dispensing
tool, follow the
instructions.




Insert tool.
Check tool.
Reboot and try again.
If error occurs again: Call
local Eppendorf Service.
 Call local Eppendorf
Application Support.
167
168
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
Cause
0x0C02
Volume too small for the Volume to be dispensed is too
dispensing tool.
small for the selected tool.
Possible causes:
• Errors in tool files.
• Errors in liquid type files.
Solution
 Call local Eppendorf
Application.
0x0C08
Tool dimension unknown Tool dimension values
 Make sure that all labware
unknown. Labware outdated or
is of the latest version.
corrupt.
0x120A
Program aborted by user User pressed the Abort button
during program run.
 Error message was
0x1221
The hood was opened
while the program was
stopped
 Close hood.
0x1222
Transfer allowance was See error message.
prematurely deactivated
during program
initialization
0x1281
Vacuum not reached
The vacuum chamber or rack is  Insert another rack.
leaky or no rack is inserted.
 In other cases, call local
Eppendorf Service.
0x1283
Vacuum not ready
No vacuum chamber available  Integrate or connect
or vacuum chamber not ready.
vacuum chamber, or:
 Call local Eppendorf
Service.
0x128B
Carrier: steps lost in x
0x128C
Carrier: final position in y
not found
See error message.
information for the user
that he aborted the
program.
 Start program again.
• Carrier collision.
• Carrier mechanics out of
order.
 Ensure that the worktable
• Problems in carrier
movement in y-axis
(sluggish movement or no
movement at all).
• Light barrier for carrier in
y-axis defective.
 Call Eppendorf Service.
equipment in epBlue is
identical to the equipment
in epMotion, then restart
the application.
 If the error persists, call
Eppendorf Service.
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
0x128E
Carrier: steps lost in y
0x1294
0x1295
Carrier: steps lost in z
Carrier:
Steps lost in z before
picking up tip.
Cause
Solution
• Carrier collision.
• Carrier mechanics out of
order.
 Ensure that the worktable
• Carrier collision.
• Carrier mechanics out of
order.
 Ensure that the worktable
• Tip was still on pipette tool
when tool started to pick up
a new tip.
• Tip rack not placed correctly
on the worktable.
• Mechanical problems of
carrier.
 Remove tips from tools.
 Place tip rack correctly,
equipment in epBlue is
identical to the equipment
in epMotion, then restart
the application.
 If the error persists, call
Eppendorf Service.
equipment in epBlue is
identical to the equipment
in epMotion, then restart
the application.
 If the error persists, call
Eppendorf Service.
and plane, on the
worktable.
In other cases:
 Call local Eppendorf
Service.
0x1296
Maximum number of
tool cycles exceeded
See error message.
 Have the tool serviced.
0x1297
Danger of collision
When running the programmed
application, the tool carrier
system touches racks or other
labware on the worktable; e.g.,
during pipetting, the optical
sensor may touch a long tube
on the adjacent position;
possible reasons:
• A low plate (microplate) is
located next to a high Tube
Rack.
• The 50 μL or 300 μL tip is
programmed to move almost
to the bottom of a very long
tube with another long tube
in the adjacent position.
 Program the labware on
the worktable so that high
and low labware is not
adjacent.
 Program the labware so
that the 30 μL or 300 μL
tip does not have to move
deeply into a long vessel.
 If possible: use higher
volumes in the long
vessels.
 If possible: use longer tips
for the long vessels.
0x1298
Tool not calibrated
Tool not calibrated
Have the tool serviced.
0x1299
Invalid number of
samples
Value for number of samples
not permissible.
 Insert an admissible value
Tip too small
Reagent Transfer: Used tip is
too small.
 Use a larger tip.
0x129A
for number of samples.
169
170
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
Cause
Solution
0x129B
Source vessel too small
Reagent Transfer: Used source
vessel is too small.
 Use a larger vessel.
0x12A2
Check levels in vacuum
command:
Wells in filter plate not
empty
Wells of the filter plate plug
while applying vacuum.
 Follow the instructions on
0x12A4
Labware in vacuum
manifold is not
composed of three parts
(filter plate, frame,
collection labware)
When applying vacuum three  See explanation in
items must be in the vacuum
"Cause".
unit (filter plate above vacuum
frame above collection plate or
tub).
0x12A5
Well pattern of upper
Vacuum unit: Well geometry of  See explanation in
and lower rack do not fit filter plate and collection plate
"Cause".
must correspond to each other
(e.g., 96 well filter plate and 96
well collection plate).
Exception: Collection plate may
be replaced by a tub.
0x12B4
Temperature unit in
location C1 not active
In the wait command, the wait  Insert temperature
command before wait
for temperature parameter was
command or delete the
edited for location C1 even
wait for temperature
though no temperature
parameter for this
command for C1 had been
location.
edited before.
0x12B5
Temperature unit in
location C2 not active
In the wait command, the wait  Insert temperature
command before wait
for temperature parameter was
command or delete the
edited for location C2 even
wait for temperature
though no temperature
parameter for this
command for C2 had been
location.
edited before.
0x12B6
Temperature unit in
location C3 not active
In the wait command, the wait  Insert temperature
command before wait
for temperature parameter was
command or delete the
edited for location C3 even
wait for temperature
though no temperature
parameter for this
command for C3 had been
location.
edited before.
0x12B7
Temperature unit in
location C1 too hot for
labware (temperature
command in this
method)
The temperature of location C1  Select a labware item that
is more resistant to high
(according to a temperature
temperatures (e.g., made
command in this application) is
from polypropylene or
too high for the labware placed
polycarbonate).
on this location.
the display.
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
Cause
Solution
0x12B8
Temperature unit in
location C2 too hot for
labware (temperature
command in this
method)
The temperature of location C2  Select a labware item that
is more resistant to high
(according to a temperature
temperatures (e.g., made
command in this application) is
from polypropylene or
too high for the labware placed
polycarbonate).
on this location.
0x12B9
Temperature unit in
location C3 too hot for
labware (temperature
command in this
method)
The temperature of location C3  Select a labware item that
(according to a temperature
is more resistant to high
command in this application) is
temperatures (e.g., made
too high for the labware placed
from polypropylene or
on this location.
polycarbonate).
0x12BA
Temperature unit in
location C1 too hot for
labware (temperature
command in previous
method)
The temperature of location C1  Select a labware item that
(according to a temperature
is more resistant to high
command in a previous
temperatures (e.g., made
application with parameter keep
from polypropylene or
temperature after method run
polycarbonate).
 Change previous
set active) is too high for the
application to not maintain
labware placed on this location.
temperature after
application run.
0x12BB
Temperature unit in
location C2 too hot for
labware (temperature
command in previous
method)
The temperature of location C2  Select a labware item that
(according to a temperature
is more resistant to high
command in a previous
temperatures (e.g., made
application with parameter keep
from polypropylene or
temperature after method run
polycarbonate).
 Change previous
set active) is too high for the
application to not maintain
labware placed on this location.
temperature after
application run.
0x12BC
Temperature unit in
location C3 too hot for
labware (temperature
command in previous
method)
The temperature of location C3  Select a labware item that
(according to a temperature
is more resistant to high
command in a previous
temperatures (e.g., made
application with parameter keep
from polypropylene or
temperature after method run
polycarbonate).
 Change previous
set active) is too high for the
application to not maintain
labware placed on this location.
temperature after
application run.
0x12D0
Parameter conflict:
Sample transfer with elution
Elution volume too large from filter option:
for this tool
Volume to be aspirated is too
large for the tip used.
 Select a tip large enough
for picking up the liquid as
well as the additional
volume of air to be
aspirated when using this
option.
171
172
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
Cause
Solution
0x12D1
Parameter conflict:
Option elution from filter:
Elution volume too large volume is too large for the
for destination vessel or vessel used.
well
 Select a tool large enough
0x12E1
Parameter conflict:
See error message.
Pre-wetting not possible
when aspirate from
bottom is selected
 Change application.
0x12E2
Parameter conflict:
See error message.
Pre-wetting not possible
when dispense from top
is selected
 Change application.
0x12E3
Parameter conflict:
Pre-wetting not possible
when elution from filter
is selected
A liquid type using a
pre-wetting step (e.g., ethanol
98%) cannot be used in
combination with the elution
from filter parameter in a
sample transfer command.
 Change application.
0x12E4
Transport of labware:
danger of collision
Danger of collision when
transporting a stack of several
racks. The upper rack would
collide with the carrier.
 Do not stack more than 5
0x12E6
Level too high
The liquid level would be higher  Adjust the liquid to be
than the vessel after dispensing.
dispensed to the vessel.
0x12E7
Opening the hood is not See error message.
allowed when putting
down tool.
Switch off power, then
switch on again to restart
method.
 See error message.
0x12F3
The Eppendorf
ThermoMixer module is
too hot for the labware.
Temperature command
in this method.
The thermal module of the
Eppendorf ThermoMixer
module is is too hot for the
selected labware.
Choose a lower temperature.
0x12F4
The Eppendorf
ThermoMixer module is
too hot for labware.
Temperature command
in previous method.
The thermal module of the
Eppendorf ThermoMixer
module is is too hot for the
selected labware.
Choose a lower temperature.
0x12F7
Waiting for Eppendorf
ThermoMixer module
The application is waiting for
the Eppendorf ThermoMixer
module.
Wait until Eppendorf
ThermoMixer module
function has finished.
when using this option.
racks in a location.
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
Cause
Solution
0x12F8
The selected mixing
speed is not possible
with this labware
The mixing speed is not
allowed for the selected
labware.
Select another labware or
mixing speed.
0x1500
Too big vessel index in
location:
A tube is to be accessed for
 Error during creation of
which the index is greater than
the application.
the number of tubes on the
plate/rack/holder.
0x1503
Tool not placed ...
Optical sensor:
A tool that is needed for the
application run was not found
on the worktable.
 Put the tool on the tool
<rack name> is not
accessible for tools in
location ...
Rack is a lower part of a
labware stack. Therefore, the
tool has no access.
 Change application so that
Liquid volume too large
for vessel in location:...
Total volume supplied in a
source vessel is larger than
needed or larger than vessel.
 Supply less volume in the
0x1504
0x1509
holder of the worktable.
the rack is accessible.
vessel.
 Change application.
 When verifying the total
volume needed for the
source or destination, take
into account the additional
aspirated volume when
using multidispense
mode.
 Calculate the total volume
Total volume supplied by the
for the source or
user in a source vessel is
destination vessel and
smaller than the volume needed
select a suitable vessel.
for a sample transfer, reagent
For additional aspirated
transfer or mix command (total
volume with
volume = volume to be
multidispense mode, refer
aspirated + remaining volume
to manual.
for this vessel + (in case of
multidispense mode:) additional  Consider that the software
may calculate higher
aspirated volume.
remaining volumes in
some cases to avoid
crashes.
 Set Detect volumes to off
for racks that are in park
positions at the beginning
of the procedure.
0x150A
Liquid volume too low
for vessel in location: ...
0x150B
Optical sensor:
See above (error 0x150A).
Liquid volume too low in
location: ...
 See above (error 0x150A).
173
174
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
Cause
Solution
0x150D
Optical sensor: Plate
could not be found in
location: ...
Optical sensor: Rack
could not be found in
location: ...
The rack programmed for this  Place the rack onto the
location could not be found by
locations as edited in the
the optical sensor; possible
corresponding
causes:
application, or:
 Make sure that the rack is
• Rack not placed onto
placed plane on the
location (wrong rack code or
worktable surface, or:
wrong rack height).
• Rack in wrong orientation.  Rotate rack 180° (front to
back) and place it back
• Problems related to the
onto the worktable
optical sensor function.
location, or:
 Call local Eppendorf
Service.
0x150E
Optical sensor:
Tips could not be found
in location ...
The tip rack programmed for
 Place the tip rack onto the
this location could not be found
locations as edited in the
by the optical sensor; possible
corresponding
causes:
application, or:
 Call local Eppendorf
• Tip rack not placed onto
Service.
location.
• Problems related to the
optical sensor function.
0x1510
Optical sensor: Nothing
could be found in
location:
See error message.
 Place the labware
programmed for this
location on the worktable.
If error occurs again:
 Call local Eppendorf
Service.
0x1512
Tip type ... is not placed Tips that are needed according  Place the tip tray
on the worktable
to the application are not
programmed for this
available on the worktable.
location on the worktable.
If error occurs again:
 Call local Eppendorf
Service.
0x1513
Position is out of range
The position to be addressed by  Change application.
the tool carrier is outside of its If error occurs again:
available range.
 Call local Eppendorf
Possible cause: Rack in park
Service.
position is programmed to be
addressed by the dispensing
tool.
0x1514
Optical sensor: Rack in
wrong orientation in
location ...
The tub holder has been placed  Rotate tub holder 180°
on the worktable in the wrong
and place it back onto the
direction.
worktable; restart the
application.
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
Cause
Solution
0x1515
Tool cannot be used for
rack in location ...
Distance between tip cones of  Change application.
the liquid handling tool does
not match the distance between
vessels (e.g., 24 tubes - rack
does not fit the eight-channel
dispensing tool).
0x1516
 Place the vessel/rack
No vessel in location: ... Vessels that are needed
combination programmed
according to the application are
for this location on the
not available on the worktable
worktable.
(vessel/rack combination).
If error occurs again:
 Call local Eppendorf
Service.
0x1518
Rack is not tangible in
location: ...
The item cannot be picked up
by the gripper.
0x1519
Tip is too thick for vessel Diameter of the destination
in location: ...
vessel is too small for the tip
when dispensing the liquid.
 Change application.
 Select other tips or vessels
in the application.
 Select dispense from top in
the options of the liquid
handling command.
0x151A
Optical sensor:
The optical sensor has detected  Remove the cap from the
There is a cap on vessel a cap on a vessel when trying to
vessel and start the run
in location: ...
detect a liquid level.
again.
0x151B
Optical sensor: There is
an incorrect vessel in
location: ...
0x151C
Optical sensor:
Level detection for very high
Vessel too high for level vessels is not possible.
detection in location: ...
Relates to vessels that are
 Place the vessel onto the
equipped with a readable code
location as edited in the
(e.g., Eppendorf tubs): The rack
corresponding
programmed for this location
application, or:
 Call local Eppendorf
could not be found by the
Application Support.
optical sensor; possible causes:
• Wrong vessel.
• Problems related to the
optical sensor function.
 Switch off the level
detection for this vessel.
 Use level detection only
for vessel/rack equipment
with a total height below
103 mm.
0x151E
Detected volume is out of Normally a system/hardware
 Do not fill vessels above
detection range ...
error (malfunction of the optical
the specified maximum
sensor); but may also be caused
filling volume.
by filling a vessel up to the total In other cases:
vessel height.
 Call local Eppendorf
Service.
175
176
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
Cause
Solution
0x151F
Labware stack too high
in location:
Maximum pieces which
may be piled:
A maximum of 5 racks can be  Do not stack more than 5
stacked in a location. Placing
racks in a location.
more than 5 racks in a location.
0x1522
Transport of the selected
labware to this position
or stack is not allowed
Position xxx
Different reasons to forbid this  Change application.
transport; main reason:
transport of the selected
labware to this position or stack
might result in instability of the
stack, or the resulting stack
might become too high.
0x1523
Labware stack too high
in location: xxx
Maximum height: xxx
mm
Total height of labware stacks
(like plates on adapters) is too
high.
 Edit application to avoid
too high labware stacks.
0x1525
Filter plate wells are not Using the check levels option in  Follow the instructions on
empty
the vacuum command, the
the display screen.
optical sensor detected residual
liquid in one of the filter wells.
0x1526
No open position
available for depositing
tool
When trying to deposit the
dispensing tool after use the
tool holder did not find a free
position for the tool.
 Clear at least one position
Method program may
not use more than four
dispensing tools
See error message.
 If more than 4 dispensing
0x1528
on the worktable to accept
a dispensing tool.
tools are needed, divide
the application into two
applications that use no
more than 4 dispensing
tools.
Dispensing tool could
See error message.
not be found.
Dispensing tool required
for this method:...
 Provide the dispensing
Gripper could not be
found
See error message.
 Provide the gripper on the
0x152D
Tip too short
Select other tips or
vessels in the method.
Tip does not reach the liquid
 Select other tips or vessels
level at the beginning or during
in the application.
the course of the liquid
handling command.
0x152F
Labware stack too high
in location: xxx
Maximum pieces which
may be piled: 5
A maximum of 5 racks can be
piled in a location. After a
transport command, the
labware stack would be too
high.
0x1529
0x152A
tool that is needed on the
worktable at its proper
position.
worktable at its proper
position.
 Change application.
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
Cause
Solution
0x1530
Dispensing in pile not
allowed in location: xxx
See error message.
 Change application.
0x1531
Transport of this pile not Do not transport a stack.
allowed in location: xxx
 Change application.
0x1581
Optical sensor:
Error in level detection.
Liquid level could not be
detected in location: ...
 Repeat measurement.
0x1602
Program too long
 Delete commands that are
Maximum size of steps is
reached.
not necessary before
insert new commands.
0x1700
Liquid volume too low
for vessel in location: ...
Total volume supplied by the
 Calculate the total volume
user in a source vessel is
for the source or
smaller than the volume needed
destination vessel and
for a sample transfer, reagent
select a suitable vessel.
transfer or mix command (total
For additional aspirated
volume = volume to be
volume with
aspirated + remaining volume
multidispense mode, refer
for this vessel + (in case of
to manual.
multidispense mode:) additional  Consider that the software
may calculate higher
aspirated volume.
remaining volumes in
some cases to avoid
crashes.
 Set Detect volumes to off
for racks that are in park
positions at the beginning
of the procedure.
0x1701
Liquid volume too large
for vessel in location:...
Total volume supplied in a
source vessel is larger than
needed or larger than vessel.
 Supply less volume in the
vessel.
 Change application.
 When verifying the total
volume needed for the
source or destination, take
into account the additional
aspirated volume when
using multidispense
mode.
epMotion 5070 only:
 Set Detect volumes to off
for racks that are in park
positions at the beginning
of the procedure.
177
178
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
Cause
Solution
0x1904
The following labware
has been deleted: ...
Edit mode:
 Change the source or
The worktable was changed
destination in the
after an application had been
parameter of the
programmed; thus, the labware
respective command in
defined in a command is no
order to match the
longer available.
worktable. In this case the
pattern also has to be
re-edited.
 The labware has to be
reprogrammed in the
worktable.
0x1908
The method was written See Error message.
with a newer program
structure. If you want to
edit this method, you
need to update your
software.
 Update your software, or:
 Call local Eppendorf
Service.
0x190D
The following labware is Edit mode/worktable:
 Select the respective
not selected in the
The chosen labware is not
labware in the Labware
Labware File window: ... available in the labware
File window. You need to
collection that had been
have the appropriate user
selected for your lab. Possible
rights. If you do not have
cause for this error message:
the necessary user rights
The labware has been
ask your administrator.
deselected in the Labware File
window.
0x190E
 Select the respective
The following tool is not Edit mode/procedure:
labware in the Labware
selected in the labware The chosen tool is not available
File window. You need to
file window: ...
in the labware collection that
have the appropriate user
had been selected for your lab.
rights. If you do not have
Possible cause for this error
the necessary user rights
message: The labware has been
ask your administrator.
deselected in the Labware File
window.
0x1910
The position of the
following labware is not
available on this
worktable.
The method was written
for another workstation
configuration.
 Set the respective labware
Edit mode/worktable: The
in the node epMotion.
chosen labware is not available
in the labware collection that
had been selected for your lab
in the software node epMotion.
Possible cause for this error
message: The labware has been
deselected in the epMotion
node.
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
Cause
Solution
0x1910
The method was written
for another workstation
configuration.
The position of the
following labware is not
available on this
worktable.
 Load the concerned
The position of required
application on a
labware is not available on this
compatible device, or
device, e.g., you have a 5075
 Modify the application
LH and the application was
until it matches the
written on a 5075 VAC.
available device.
The position of the
following labware is no
longer allowed
yyy
in location: xxx
Edit mode/worktable: The
 Place the respective
chosen labware may not be
labware on another
placed on the selected position
position.
anymore. Possible cause for this
error message: The application
has been written with a former
version of the software.
0x1911
The following labware
has been changed, so the
pattern no longer fits:
xxx
 Either change the order or
Because you can change the
contents of the Tubs +
order or contents Tubs +
Modules(equip) + Holders
Modules(equip) + Holders
combination back to the
combination, the recent pattern
original or change the
of a command may not fit the
pattern in the command.
new positions of the tubes.
0x1911
The following labware
has been changed, so the
pattern no longer fits:
xxx
Because the order or contents
of a “Equipped Holder”
combination can be changed,
the recent pattern of a
command may not fit the new
positions of the tubes.
 Either change the order or
contents of the “Equipped
Holder” combination back
to the original or change
the pattern in the
command.
 Enter a different name.
0x1A02
The name is already used Edit mode/labware:
for another labware
The same name has been
defined for a different rack or
another labware item.
0x1A03
This position is not
Edit mode/worktable:
 Place the selected labware
available for the selected Certain worktable positions are
in another location.
labware
not allowed for specific labware
(e.g., tips can only be placed in
the rear of the worktable).
0x1A04
The selected labware
may not be stacked on
top of labware already
placed
Edit mode/worktable:
Building of labware stacks on
the worktable is restricted to
certain labware combinations
(e.g., thermorack above
thermorack does not make
sense).
0x1A05
Maximum number of
labware stacked: 5
Edit mode/worktable:
Place the selected labware in
Certain labware build a pile
another location.
which contains too many when
placed on certain labware.
 See "Cause".
179
180
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
Cause
Solution
0x1A06
Labware stack too high
in location: xxx
Maximum height: xxx
mm
Edit mode/worktable:
 See "Cause".
Labware stacks on the
worktable may not exceed a
maximum height limit (e.g.,
plates on adapters is allowed;
reservoir holder on adapters is
not allowed because the stack
would be too high).
0x1A10
Eight-channel
dispensing tool cannot
be used for this source
rack
 Choose another rack or
Edit mode/parameter in
another tool.
command Sample Transfer:
Source rack does not fit the
eight-channel dispensing tool
(e.g., 24-well plate or tube rack
with 24 positions).
0x1A10
Eight-channel
dispensing tool cannot
be used for this source
rack
 Choose another rack or
Edit mode/parameter in
another tool.
command Sample Transfer:
Source rack does not fit to
eight-channel dispensing tool
(e.g.: 24-well-plate or tube rack
with 24 positions).
0x1A11
Eight-channel
dispensing tool cannot
be used for this
destination rack.
 Choose another rack or
Edit mode/parameter in
another tool.
command Sample Transfer:
Destination rack does not fit the
eight-channel dispensing tool
(e.g., 24-well plate or tube rack
with 24 positions).
0x1A12
No source or destination Edit mode/parameter in
selected
command Sample Transfer:
Source or destination rack has
not been selected.
 Select source or
destination, respectively.
0x1A15
Invalid entry for
movement blow (0 ...
100)
Edit mode/parameter in transfer  Enter a value between 0
command:
and 100%.
A value beyond the allowed
range has been entered for the
parameter Movement Blow.
0x1A15
Invalid entry for
movement blow (0 ...
100)
Edit mode/parameter in transfer  Enter a value between 0
command:
and 100%.
A value beyond the allowed
range has been entered for the
parameter Movement Blow.
0x1A16
Invalid entry for delay
blow (0 ... 9999)
Edit mode/parameter in transfer  Enter a value between 0
command:
and 9999 msec.
A value beyond the allowed
range has been entered for the
parameter Delay Blow.
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
Cause
Solution
0x1A16
Invalid entry for delay
blow (0 ... 9999)
Edit mode/parameter in transfer  Enter a value between 0
and 9999 msec.
command:
A value beyond the allowed
range has been entered for the
Delay Blow parameter.
0x1A17
Invalid entry for speed
aspiration (0.2 ... 110)
Edit mode/parameter in transfer  Enter a value between 0.2
command:
and 110 mm/sec.
A value beyond the allowed
range has been entered for the
parameter Speed Aspiration.
0x1A17
Invalid entry for speed
aspiration (0.2 ... 110)
Edit mode/parameter in transfer  Enter a value between 0.2
command:
and 110 mm/sec.
A value beyond the allowed
range has been entered for the
speed aspiration parameter.
0x1A18
Invalid entry for speed
dispense (0.2 ... 110)
Edit mode/parameter in transfer  Enter a value between 0.2
command:
and 110 mm/sec.
A value beyond the allowed
range has been entered for the
parameter Speed Dispense.
0x1A18
Invalid entry for speed
dispense (0.2 ... 110)
Edit mode/parameter in transfer  Enter a value between 0.2
command:
and 110 mm/sec.
A value beyond the allowed
range has been entered for the
parameter Speed Dispense.
0x1A19
Invalid entry for speed
blow (0.2 ... 110)
Edit mode/parameter in transfer  Enter a value between 0.2
command:
and 110 mm/sec.
A value beyond the allowed
range has been entered for the
parameter Speed Blow.
0x1A19
Invalid entry for speed
blow (0.2 ... 110)
Edit mode/parameter in transfer  Enter a value between 0.2
command:
and 110 mm/sec.
A value beyond the allowed
range has been entered for the
parameter Speed Blow.
0x1A1A
Invalid entry for initial
stroke (0 ... 100)
Edit mode/parameter in transfer  Enter a value between 0
command: a value beyond the
and 100%.
allowed range has been entered
for the parameter initial stroke.
0x1A20
Eight-channel
dispensing tool cannot
be used for this source
rack
Edit mode/parameter in
 Choose another rack or
command Reagent Transfer:
another tool.
Source rack does not fit the
eight-channel dispensing tool
(e.g., 24-well plate or tube rack
with 24 positions).
181
182
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
0x1A21, 0x1A30, Eight-channel
0x1A31, 0x1A51 dispensing tool cannot
be used for this
destination rack
Cause
Solution
Edit mode/parameter in the
 Choose another rack or
affected command:
another tool.
Destination rack does not fit the
eight-channel dispensing tool
(e.g., 24-well plate or tube rack
with 24 positions).
0x1A22, 0x1A32, No source or destination Edit mode/parameter in the
0x1A42, 0x1A52 selected
affected command:
Source or destination rack has
not been selected.
 Select source or
destination, respectively.
0x1A40, 0x1A41, Eight-channel
0x1A50
dispensing tool tool
cannot be used for this
source rack
Edit mode/parameter in the
 Choose another rack or
affected command:
another tool.
Source rack does not fit the
eight-channel dispensing tool
(e.g., 24-well plate or tube rack
with 24 positions).
0x1A61
Eight-channel
dispensing tool cannot
be used for this rack
 Choose another rack or
Edit mode/parameter in
another tool.
command Mix:
Rack does not fit the
eight-channel dispensing tool
(e.g., 24-well plate or tube rack
with 24 positions).
0x1A62
No rack selected
Edit mode/parameter in
command Mix:
Rack has not been selected.
0x1A65
Invalid entry for speed
(0.2 ... 110)
Edit mode/parameter in
 Enter a value between 0.2
command Mix:
and 110 mm/sec.
A value beyond the allowed
range has been entered for the
parameter Speed.
0x1A70
This position is already
occupied
Edit mode/pattern:
When editing the pattern you
have tried to select a certain
position that is already
occupied.
0x1A76
 Select rack, respectively.
 Follow the direction of the
edited pattern and move
to a different position.
eight-channel dispensing Edit mode/pattern:
 Choose another rack or
tool cannot be used for Rack does not fit the
another tool.
this ReservoirRack.
eight-channel dispensing tool
(e.g., Tubs + Modules (equip) +
Holders combination with
positions all less than 8 in
modules).
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
Cause
Solution
0x1A77
No ReservoirRack or
tubes found
 Choose another rack.
Edit mode/pattern:
Rack does not have any
positions (e.g., Tubs + Modules
(equip) + Holders-combination
with positions all less than 1 in
Modules).
0x1A78
Number of tubes not
supported
 Choose another rack.
Edit mode/pattern:
One or more Modules have 3, 5,
6, 7 or more than 8 positions.
This is not supported.
0x1A80
Invalid entry for minutes Edit mode/parameter in
 Enter a value between 0
(0 ... 99)
command Wait:
and 99 minutes.
A value beyond the allowed
range has been entered for the
parameter minutes.
0x1A81
Invalid entry for seconds Edit mode/parameter in
 Enter a value between 0
(0 ... 59)
command Wait:
and 59 seconds.
A value beyond the allowed
range has been entered for the
parameter seconds.
0x1A90
Selecting more than one
rack as Source or as
Destination:
All source racks (or all
destination racks, resp.)
must have the same well
pattern
Edit mode/parameter in transfer  See explanation in
"Cause".
command:
If more than one rack is
selected as source (or
destination) all source (or
destination), racks must have
the same well pattern (e.g.,
96-well plates).
0x1A90
Selecting more than one
rack as Source or as
Destination:
All source racks (or all
destination racks, resp.)
must have the same well
pattern
Edit mode/parameter in transfer  Choose another rack.
command:
The selected labware have a
different amount of wells.
0x1AA0
Invalid entry for
temperature (0 ... 110)
 Enter a value between 0
Edit mode/parameter in
and 110 degrees.
command Temperature:
A value beyond the allowed
range has been entered for the
parameter Temperature.
0x1AB0
Invalid entry for minutes Edit mode/parameter in
 Enter a value between 0
(0 ... 99)
command Vacuum:
and 99 minutes.
A value beyond the allowed
range has been entered for the
parameter minutes.
183
184
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
0x1AB1
Invalid entry for seconds Edit mode/parameter in
 Enter a value between 0
(0 ... 59)
command Vacuum:
and 59 seconds.
A value beyond the allowed
range has been entered for the
seconds parameter.
0x1AB2
Invalid entry for vacuum
pressure (1 ... 90) kPa
and (10 ... 900) mbar
resp.
Edit mode/parameter in
 Enter a value between 0
command Vacuum:
and 59 seconds.
A value beyond the allowed
range has been entered for the
seconds parameter.
0x1AE0
Invalid entry for mixing
speed (0 ... 2000)
Edit mode/parameter in
Enter a value between 0 and
command Thermomixer: a value 2000 rpm
beyond the allowed range has
been entered for the parameter
speed
0x1AE1
Enter a value between 0 and
Invalid entry for minutes Edit mode/parameter in
(0 ... 120)
command Thermomixer: a value 120 minutes
beyond the allowed range has
been entered for the parameter
minutes
0x1AE2
Enter a value between 0 and
Invalid entry for seconds Edit mode/parameter in
(0 ... 59)
command Thermomixer: a value 59 seconds
beyond the allowed range has
been entered for the parameter
seconds
0x1AE3
Invalid entry for
temperature (4 ... 110)
0x1C0B
Sample number too large Run mode:
The number of samples you
entered will fill more than one
rack (source or destination,
respectively) based on the
programmed pattern.
0x1C0D
You must clear old
pattern first
Press new pattern
Cause
Solution
Edit mode/parameter in
Enter a value between 4 and
command Thermomixer: a value 110 degrees
beyond the allowed range has
been entered for the parameter
temperature
 Start the application again
and enter a lower number
of samples; or:
 Enter the edit mode and
program a pattern that
together with the number
of samples you want to
run will not extend beyond
one rack.
 Delete the old pattern by
Edit mode/pattern:
pressing the New pat.
You tried to change a stored
softkey
pattern before deleting the old
pattern.
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
Cause
Solution
0x1C0D
You must clear old
pattern first
Press "new pattern"
 Delete the old pattern by
Edit mode/pattern:
pressing the button new
You tried to change a stored
pattern.
pattern before deleting the old
pattern.
0x1C0E
You must go forward
Edit mode/pattern:
 See explanation in
When entering the pattern, the
"Cause".
order of edited locations in the
source (or destination,
respectively) must be from left
to right or from the top of the
pattern downwards (i.e., move
only in columns or in rows).
0x1C0F
 See explanation in
You may only move
Edit mode/pattern:
"Cause".
horizontally or vertically When entering the pattern the
order of edited locations in the
source (or destination, resp.)
must be from the left to right or
from top of the pattern
downwards (i.e., move only in
columns or in rows).
Note: Error message may also
occur when working with an
eight-channel dispensing tool
and editing another position
than the upper ones (see error
code 0x1C1F).
0x1C10
Pattern for replicates of Edit mode/pattern:
first sample too complex The pattern algorithm cannot
handle this pattern.
 Enter a simpler pattern if
possible.
In case this is not possible:
 Call local Eppendorf
Application Support.
0x1C11
Pattern too complex
Edit mode/pattern:
 Enter a simpler pattern if
The pattern algorithm cannot
possible.
handle this pattern.
In case this is not possible:
Note: See note in error 0x1C0F.
 Call local Eppendorf
Application Support.
0x1C14
Pattern must fit in rows
or columns
Edit mode/pattern:
 Enter a simpler pattern if
The basic unit of the pattern
possible.
you tried to enter extends
In case this is not possible:
beyond a row or a column. This
 Call local Eppendorf
cannot be handled by the
Application Support.
pattern algorithm.
185
186
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
Cause
0x1C15
Pattern too complex
Edit mode/pattern:
 Enter a simpler pattern if
The pattern algorithm cannot
possible.
handle this pattern.
In case this is not possible:
Note: See note in error 0x1C0F.
 Call local Eppendorf
Service.
0x1C16
This position is already
occupied
Edit mode/pattern:
When editing the pattern you
have tried to select a certain
rack position that is already
occupied.
 Following the edited
You must start with the
source
Edit mode/pattern:
When editing a pattern you
must start with the source.
 See explanation in
Please enter a source
now
Edit mode/pattern:
In the destination rack, you
tried to enter more replicates
than you had sources.
 Enter the same number of
0x1C19
Please enter a
destination now
Edit mode/pattern:
 Press the Destination
When having selected a source
softkey and edit the
in the Sample Transfer
destination position(s) for
command you first have to
the selected source
enter a destination for this
position.
source before moving to the
next source position.
0x1C1A
No more positions
available (limited by
Number of Samples
command)
 Select a pattern that fits
Edit mode/pattern:
the programmed Number
Editing further positions is not
of Samples command.
possible because the limit set in
the Number of Samples
command would be exceeded.
0x1C1E
Pattern for reagent
transfer:
Source can be chosen
only once
Edit mode/pattern for command  Enter the source only
Reagent Transfer:
once. In case this does not
After having entered the source
meet your requirements
and the destinations for the
for this application
reagent transfer you cannot
consider selecting
select an additional source.
command Sample Transfer
instead of Reagent
Transfer, or:
 Call local Eppendorf
Application Support.
0x1C1F
Pattern with
eight-channel dispensing
tool:
Please edit upper
position of this tool
Edit mode/pattern with
 See explanation in
eight-channel dispensing tool:
"Cause".
Only the upper positions of the
eight-channel dispensing tool
can be selected.
0x1C17
0x1C18
Solution
pattern move to a different
rack position.
"Cause".
replicates for all sources
you edit when
programming a pattern.
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
Cause
Solution
0x1C20
Pattern for sample
transfer:
only one position per
sample on source
Edit mode/pattern for command  Enter destination for the
source you just selected;
Sample Transfer:
afterwards, you can edit
Before selecting a second
the next source position.
source position, you have to
edit the destination for the first
source position.
0x1C21
In source rack further
positions cannot be
edited because positions
in destination rack are
already occupied
Edit mode/pattern:
Selecting further source
positions would require a
second destination rack
according to the pattern you
edited.
 Edit a pattern that does
not require more than one
destination rack per
command. To use more
destination racks, create
additional commands.
0x1C22
Pattern for pool one dest: Edit mode/pattern for command  Enter the destination only
once. In case this does not
destination can be
Pool one destination:
meet your requirements
chosen only once
After having entered the
for this application,
sources and the destination,
consider selecting
you cannot select an additional
command Pool instead of
destination.
Pool one destination, or:
 Call local Eppendorf
Application Support.
0x1C23
Pattern for dilute:
only one position per
sample on source
Edit mode/pattern for command  Enter destination for the
Dilute:
source you just selected;
Before selecting a second
afterwards, you can edit
source position, you have to
the next source position.
edit the destination for the first
source position.
0x1C25
Pattern for pool:
only one position per
sample on destination
Edit mode/pattern for command  Enter sources for the next
destination position;
Pool:
afterwards, you can edit
Before selecting a second
the next destination
destination position, you have
position.
to edit the next source positions
to be pooled into this
destination.
0x1C26
Pattern for Reagent
Transfer: not enough
source positions
Run mode: To provide enough  Start the application again
and enter a lower number
reagent volume for the number
of samples; or:
of samples you entered, the
 Enter the edit mode and
selected reagent source
program more reagent
positions must be higher.
source positions in the
pattern. Keep in mind that
the selected reagent
source positions may not
extend beyond one rack.
0x2025
Bottom tolerance too
large
Bottom tolerance too big.
 Use a smaller value.
187
188
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
Cause
Solution
0x2026
Bottom tolerance too
small
Bottom tolerance too small.
 Use a bigger value.
0x2101
Tool not defined.
Parameter Pipet. tool was not
edited in the application.
 See explanation in
"Cause".
0x2104
Tips not edited in
worktable/procedure
Tips were edited in the
 Edit the tips that you
procedure of the application,
programmed in the
but they were not edited in the
procedure in the
worktable (e.g., filter tips <->
worktable.
tips without filter).
0x2105
Parameter conflict:
Start volume greater
than filling volume of
source tube or well
The Volume and Source
 Edit Volume and Source in
parameters of the source vessel
the worktable so that
do not match (Volume is higher
Volume is covered by the
than the maximum filling
maximum filling volume of
volume of the source vessel).
the source vessel.
0x2106
Parameter conflict: Start
volume greater than
filling volume of
destination vessel or
well.
The Volume and Destination
 Edit Volume and
parameters of the destination
Destination in the
vessel do not match (Volume is
worktable so that Volume
higher than the maximum
is covered by the
filling volume of the destination
maximum filling volume of
vessel).
the destination vessel.
0x2107
Volume not defined
Parameter Volume was not
edited in the application.
 See explanation in
"Cause".
0x2108
Parameter conflict:
The parameters Volume and
 Edit Volume and Pipet. tool
Volume too small for this Pipet. tool of the application do
so that Volume is covered
tool
not match (Volume is smaller
by the volume range of the
than the lower limit of the tool
pipet tool.
volume range).
0x2109
Parameter conflict:
The parameters Volume and
 Edit Volume and Pipet. tool
Volume too large for this Pipet. tool of the application do
so that Volume is covered
tool
not match (Volume is higher
by the volume range of the
than the upper limit of the tool
pipet tool.
volume range).
0x210A
Parameter conflict:
volume greater than
filling volume of source
tube or well
The Volume and Source
 Edit Volume and Source so
parameters of the application
that Volume is covered by
do not match (Volume is higher
the maximum filling
than the maximum filling
volume of the source
volume of the source vessel).
vessel.
0x210B
Parameter conflict:
volume greater than
filling volume of
destination vessel or
well.
The Volume and Destination
 Edit Volume and
parameters of the application
Destination so that Volume
do not match (Volume is higher
is covered by the
than the maximum filling
maximum filling volume of
volume of the destination
the destination vessel.
vessel).
0x210D
Source rack not defined Parameter Source was not
edited in the application.
 See explanation in
"Cause".
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
Cause
Solution
0x210E
Source rack not edited in The source rack you edited in  Edit the rack that you
programmed in the
worktable
the procedure of the application
procedure as Source in the
has been removed from the
worktable, or edit a
worktable.
different source rack in
the application.
0x210F
Source rack not selected The source rack you edited in
in the labware file
the application is not selected
window
or removed in the labware file
window and therefore is not
available for programming.
 Select the rack in the
labware file window or
edit a different rack in the
application.
Destination rack not
defined
Parameter Source was not
edited in the application.
 See explanation in
Destination rack not
edited in worktable
Destination rack was edited in
the procedure of the
application, but it was not
edited in the worktable.
 Edit the rack that you
0x2112
Destination rack not
selected in the Labware
File Window
The destination rack you edited  Select the rack in the
labware file window or
in the application is not
edit a different rack in the
selected or removed in the
application.
labware file window and
therefore is not available for
programming.
0x2113
Pattern not defined
Parameter Pattern was not
edited in the application.
0x2110
0x2111
"Cause".
programmed in the
procedure as Destination
in the worktable or edit a
different destination rack
in the application.
 See explanation in
"Cause".
0x2114
Loading error (invalid
entry in pattern)
Normally a system error; but
 Edit a pattern with source
may also be caused by editing a
and destination positions.
pattern without destination
In other cases:
positions; or:
 Call local Eppendorf
File damaged.
Service.
0x211B
Liquid type not defined
Parameter Liquid Type was not  Choose a liquid type.
edited in the application.
0x211D
Mixing cycles in source
not defined
Parameter No. of Cycles in a mix  See explanation in
procedure was not edited for
"Cause".
the source in the application
(mix procedure as defined in a
command Mix or as part of a
liquid transfer command via
parameter Options).
189
190
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
Cause
Solution
0x211E
Invalid entry for mixing Entry for the No. of Cycles
 Enter a number between 1
cycles in source (1 ... 99) parameter in a mix procedure
and 99 for the No. of
for source vessels was higher
Cycles parameter.
than the max. limit (1 up to 99
cycles) (mix procedure as
defined in a command Mix or as
part of a liquid transfer
command via parameter
Options).
0x211F
Invalid entry for mixing Entry for the parameter Speed
speed in source (1 ... 10) in a mix procedure for source
vessels was higher than the
max. limit (1 up to 10) (mix
procedure as defined in a
command Mix or as part of a
liquid transfer command via
parameter Options).
 Enter a number between 1
and 10 for the Speed
parameter.
0x2120
 See explanation in
Mixing volume in source Parameter Volume in a mix
"Cause".
not defined
procedure for source vessels
was not edited in the
application (mix procedure as
defined in a command Mix or as
part of a liquid transfer
command via parameter
Options).
0x2121
 Edit Volume and Pipet. tool
Parameter conflict:
The parameters Volume and
so that Volume is within
mixing volume in source Pipet. tool of a mix procedure
pipet tool's volume range.
too large for this tool
for source vessels are not in
agreement (Volume is higher
than the upper limit of the tool's
volume range) (mix procedure
as defined in a command Mix or
as part of a liquid transfer
command via parameter
Options).
0x2122
 Edit Volume and Pipet. tool
Parameter conflict:
The parameters Volume and
so that Volume is within
mixing volume in source Pipet. tool of a mix procedure
the pipet tool's volume
too small for this tool
for source vessels do not match
range.
(Volume is less than the lower
limit of the tool's volume range)
(mix procedure as defined in a
command Mix or as part of a
liquid transfer command via
parameter Options).
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
Cause
Solution
0x2123
Parameter conflict:
mixing volume in source
greater than filling
volume of source tube or
well
 Edit Volume and Source so
The Volume and Source
that Volume is within the
parameters of a mix procedure
allowable filling volume of
in the application do not match
the source vessel.
(Volume is higher than the
maximum filling volume of the
source vessel) (mix procedure
as defined in a command Mix or
as part of a liquid transfer
command via parameter
Options).
0x2124
Mixing cycles in
destination not defined
Parameter No. of Cycles in a mix  See explanation in
"Cause".
procedure for destination
vessels was not edited in the
application (mix procedure as
part of a liquid transfer
command via parameter
Options)
0x2125
Invalid entry for mixing
cycles in destination
(1 ... 99)
Entry for the parameter No. of
Cycles in a mix procedure for
destination vessels was higher
than the max. limit (1 up to
99 cycles) (mix procedure as
part of a liquid transfer
command via parameter
Options).
0x2126
Invalid entry for mixing
speed in destination
(1 ... 10)
Entry for the parameter Speed  Enter a number between 1
and 10 for the Speed
in a mix procedure for
parameter.
destination vessels was higher
than the max. limit (1 up to 10)
(mix procedure as part of a
liquid transfer command via
parameter Options).
0x2127
Mixing volume in
destination not defined
Parameter Volume in a mix
procedure for destination
vessels was not edited in the
application (mix procedure as
part of a liquid transfer
command via parameter
Options).
 Enter a number between 1
and 99 for the No. of
Cycles parameter.
 See explanation in
"Cause".
191
192
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
Cause
Solution
0x2128
Parameter conflict:
mixing volume in
destination too large for
this tool
The parameters Volume and
Pipet. tool in a mix procedure
for destination vessels do not
match (Volume is higher than
the upper limit of the tool
volume range) (mix procedure
as part of a liquid transfer
command via parameter
Options).
 Edit Volume and Pipet. tool
0x2129
Parameter conflict:
mixing volume in
destination too small for
this tool
 Edit Volume and Pipet. tool
The parameters Volume and
so that Volume is within
Pipet. tool in a mix procedure
the volume range of the
for destination vessels do not
pipet tool.
match (Volume is lower than the
minimum allowed volume) (mix
procedure as part of a liquid
transfer command via
parameter Options).
0x212A
Parameter conflict:
mixing volume in
destination greater than
filling volume of
destination vessel or
well.
 Edit Volume and
The Volume and Destination
Destination so that Volume
parameters in a mix procedure
is within the maximum
for destination vessels do not
filling volume of the
match (Volume is higher than
destination vessel.
the maximum filling volume of
the destination vessel) (mix
procedure as part of a liquid
transfer command via
parameter Options).
0x212C
Parameter conflict:
mix after dispense not
allowed in multidispense
mode
When the transfer type
parameter is set to
multidispense, the mix after
dispense parameter cannot be
edited for this command.
Parameter conflict:
eight-channel dispensing
tool cannot be used for
this source rack
Edit mode/parameter in liquid  Choose another rack or
another tool.
handling command:
Source rack does not fit the
eight-channel dispensing tool
(e.g., 24-well plate or tube rack
with 24 positions).
0x212D
so that Volume is within
the pipet tool's volume
range.
 Change parameter transfer
type to pipette, or:
 Omit the mixing step; in
this case you could also
edit another mixing step
as a new command (Mix),
which would be
performed after the
previous command of the
procedure has ended.
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
Cause
Solution
0x212E
Parameter conflict:
eight-channel dispensing
tool cannot be used for
this destination rack
Edit mode/parameter in liquid  Choose another rack or
another tool.
handling command:
Destination rack does not fit the
eight-channel dispensing tool
(e.g., 24-well plate or tube rack
with 24 positions).
0x212F
 Program a pattern that
Sample number too large The number of samples you
together with the number
have entered will fill more than
of samples you want to
one rack (source or destination,
run will not extend beyond
respectively) based on the
one rack, or choose a
programmed pattern.
lower number of samples
in the corresponding
Number of Samples
command.
0x2130
Parameter conflict: tip
cannot be used for this
source rack
The Source rack parameter in
the liquid handling command
does not match the selected
tool (e.g., 384-well plate and
TS_1000 or TM1000_8).
0x2131
Parameter conflict: tip
cannot be used for this
destination rack
The Destination rack parameter  Choose another rack or
in the liquid handling command
another tool.
does not match the selected
tool (e.g., 384-well plate and
TS_1000 or TM1000_8).
0x2132
Invalid number of
samples (1 ... 384)
Number of samples you have
entered is too high.
 Choose another rack or
another tool.
 Enter a maximum number
of samples up to 384.
0x2133
Rack or plate not defined Edit mode/parameter in
 See explanation in
command Transport:
"Cause".
Labware to be transported was
not edited.
0x2134
Rack not selected in the The rack you edited in a
 Select the rack in the
labware file window
command Transport (parameter
labware file window or
labware) is not selected or
edit a different rack in the
removed in the labware file
application.
window and therefore is not
available for programming.
0x2135
 See explanation in
Location for destination Edit mode/parameter in
"Cause".
not defined
command Transport:
The destination position for the
labware to be transported was
not edited.
193
194
epBlue - Software error messages
epBlue™ with MultiCon
English (EN)
Code
Problem
0x2136
Invalid entry for seconds Edit mode/parameter in
 Enter a value between 0
(1 ... 59)
command Wait:
and 59 seconds.
A value beyond the allowed
range has been entered for the
seconds parameter.
0x2137
Invalid entry for minutes Edit mode/parameter in
(1 ... 99)
command Wait:
A value above the allowable
maximum minutes has been
entered for the minutes
parameter.
 Enter a value between 0
Method without active
commands
Application only contains
passive commands (such as
wait, comment, etc.).
 Insert at least one active
0x2139
Parameter conflict
mix before aspirating is
not allowed in
multiaspirate mode.
Commands Pool and Pool One  Do not mix.
Destination:
Mix before aspirating not
allowed in multiaspirate mode.
0x2152
Editing Vac Lid option in
vacuum command not
possible. There is no Vac
Lid on the worktable.
To use the Vac Lid option in the  Editing the worktable
vacuum command the
please place a Vac Lid on
worktable must be equipped
position "GRIPPER".
with a Vac Lid.
0x2154
Parameter conflict:
Check levels time may
not be shorter than
remove lid time
The check levels option can only  Select a check levels time
be performed when the lid has
greater than the remove lid
been taken off the filter plate
time.
before by the gripper.
0x2170
Parameter conflict:
To edit the elution from filter
Parameter elution from option a filter plate must have
filter is only possible
been edited as source.
when filter plates have
been selected as source
 See explanations in
Parameter conflict:
See explanation in the error
Multidispense mode is
message.
not allowed when elution
from filter is selected
 See explanation in the
0x2138
0x2171
0x2172
Parameter conflict:
Transfer volume must be
set to zero when the
elution from filter
parameter has been
selected
Cause
Using the elution from filter
option, the complete volume
contained in the filter plate
wells is always aspirated;
therefore, editing a volume to
be transferred is not possible.
Solution
and 99 minutes.
command.
"Cause".
error message.
 Set the volume to zero
because the entry will not
have an effect in the
application run.
Ordering information
epBlue™ with MultiCon
English (EN)
17
17.1
Ordering information
Accessories
Order no.
(International)
5075 002.507
5075 002.515
5075 002.523
5075 002.728
5075 002.701
Order no. (North
America)
Description
LH assistant
PCR assistant
designed to streamline and automate the whole PCR set-up
workflow, like Compose Mastermix, Normalize
Concentrations, Create Dilution Series, Setup Reactions
Prep assistant
provide a step-by-step guided workflow of automated nucleic
acid purification with the MagSep reagent kits
epBlue GxP
Modification kit for epMotion with MultiCon PC as of serial
number 4000
with documentation folder
epBlue ID
Modification kit for epMotion with MultiCon PC as of serial
number 4000
with barcode reader
195
196
Index
epBlue™ with MultiCon
English (EN)
Index
A
Application
Change name .................................................100
Change properties..........................................100
Control .............................................................36
Copy ...............................................................100
Creation............................................................41
Delete .............................................................100
Entering the number of samples......................27
Export.............................................................102
Filling volume of the labware...........................33
Home................................................................35
ID entry ............................................................30
Import ............................................................101
Labware run parameters..................................29
Manage ............................................................99
Open.................................................................25
Preparation.......................................................26
Run parameters for barcode reader...........28, 29
Run parameters for optical sensor...................28
Saving as PDF ................................................103
Setting the HEPA air filter and the UV lamp....27
Assistants...............................................................18
Checksum
Create in external software ........................... 150
Export ............................................................ 149
Import ............................................................ 149
Validate in external software......................... 149
Verify ............................................................. 149
Commands
Comment.......................................................... 71
CSV import ...................................................... 83
Dilute................................................................ 65
Exchange ......................................................... 72
External process .............................................. 82
Magnetic separation........................................ 72
Mix................................................................... 73
Number of samples .......................................... 74
Pierce............................................................... 69
Pool .................................................................. 68
Pool one destination ......................................... 68
Reagent transfer ............................................... 64
Sample transfer ................................................ 54
Temperature..................................................... 75
ThermoMixer ................................................... 76
Transport.......................................................... 80
User intervention .............................................. 81
Vacuum ............................................................ 78
Wait ................................................................. 83
Audit trail
Categories ......................................................163
Display ...........................................................161
Entries ............................................161, 162, 163
Export.............................................................163
Filter.......................................162, 162, 162, 162
Print ...............................................................163
CSV files................................................................ 83
For filling volume ............................................ 87
For liquid transfer............................................ 84
Avoiding the formation of foam.............................60
Dilution series
Create ........................................................ 65, 66
Example........................................................... 66
B
Backup.................................................................153
Creation..........................................................153
Barcode reader
Settings ..........................................................142
Bottom tolerance
Setting............................................................110
C
Changing the liquid type .......................................62
D
Data backup ........................................................ 153
E
epBlue
First start ......................................................... 19
Home ............................................................... 19
Log off ............................................................. 20
Login................................................................ 21
Menu bar ........................................................ 99
Start screen ..................................................... 99
Switching off.................................................... 20
Eppendorf ThermoMixer module
Index
epBlue™ with MultiCon
English (EN)
Maximum speeds .....................................77, 137
Activate.......................................................... 107
Activating ID tracking...................................... 47
Deactivate ...................................................... 107
Delete ............................................................ 107
Detect volumes of the optical sensor ......... 94, 95
Export ............................................................ 109
Import ............................................................ 107
Labware combinations .................................. 111
Labware definition................................. 117, 118
Labware library ............................................. 117
Manage.......................................................... 106
Maximum speeds .................................... 77, 137
Naming conventions...................................... 105
Placement ........................................................ 43
Positioning on the worktable..................... 25, 28
Properties ...................................................... 109
Run parameters for the optical sensor ............ 96
Setting the bottom tolerance ......................... 110
Special features ............................................. 118
Stack ................................................................ 44
Export
Audit trail .......................................................163
Checksum.......................................................149
External process ....................................................82
F
Features.................................................................17
File menu...............................................................22
Filling volume
CSV files ...........................................................87
Folder
Manage ............................................................99
H
HEPA air filter......................................................141
Home..............................................................141
Stop ................................................................142
I
ID
Admin Lab......................................................131
Commands .....................................................127
Entry...............................................................127
Entry with entry list..........................................31
Lists................................................................125
Manual entry ....................................................30
Parameters .....................................................131
ID lists..................................................................125
Administration ...............................................128
Deletion..........................................................130
Export.............................................................130
Import ............................................................129
Input list .........................................................126
Open...............................................................129
Result list .......................................................126
ID tracking
Activating the labware .....................................47
Input list ..............................................................126
L
Labware
Labware combinations
Change........................................................... 111
Create ............................................................ 111
Labware definition
Loading from the Internet ............................. 117
Request from Eppendorf ............................... 117
Special features ............................................. 118
LH assistant .......................................................... 18
Liquid types .......................................................... 60
Log
Print ................................................................. 40
Saving as PDF.................................................. 40
M
Magnetic separation ............................................. 72
Menu bar............................................................... 99
Mixing................................................................... 73
Mouse ................................................................... 21
MultiCon PC
Switching off.................................................... 20
O
Optical sensor ....................................................... 93
197
198
Index
epBlue™ with MultiCon
English (EN)
Basic settings .................................................133
Check labware placement ................................97
Detect tips ........................................................97
Detect volumes..................................................93
Detect volumes application parameter for plates
.........................................................................94
Detect volumes for special labware ..................95
Measure filling volume ....................................93
Run parameters for labware ............................96
Load............................................................... 114
Touchscreen.......................................................... 21
U
User
Log off ............................................................. 20
Login................................................................ 21
UV
Lamp.............................................................. 140
P
Pattern
Definition .........................................................89
Irregular pattern...............................................92
Regular pattern ................................................90
PDF......................................................................103
Pierce.....................................................................69
Pipette tips
Rinse ................................................................59
Print templates ....................................................158
Procedure
Processing........................................................49
R
Rack
Load ...............................................................114
Report
Display .............................................................39
ReservoirRack
Equipping with ReservoirRack modules ........116
Equipping with Reservoirs.............................116
ReservoirRack module
Equipped with individual vessels...................114
Result list.............................................................126
S
Screen keyboard....................................................21
Start screen ...........................................................99
T
Thermorack
W
Worktable
Decontamination ........................................... 140
Positioning labware ................................... 25, 28
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