Olink Signature Q100 User Manual

Document

&reg;
Olink&reg; Signature Q100
User Manual
1172 v1.1, 2022-01-25
Table of contents
1.
About This User Manual.............................................................................................................3
1.1 Safety Alert Conventions..................................................................................................3
1.2 Safety Data Sheets..............................................................................................................4
2. System Overview...........................................................................................................................5
2.1 Components of Olink Signature Q100.......................................................................5
2.2 Olink Signature Q100 Interface Plates.......................................................................6
2.3 Supported Chips..................................................................................................................6
2.4 Supported Panels................................................................................................................ 7
2.5 Instrument Specifications................................................................................................ 7
2.6 Air Options............................................................................................................................. 7
2.7 Ethernet Option...................................................................................................................8
2.8 Regulatory Compliance....................................................................................................9
3. Olink Signature Q100 Operation.........................................................................................11
3.1 Olink Signature Q100 System Software..................................................................11
3.2 User Accounts..................................................................................................................... 22
3.3 Best Practices..................................................................................................................... 26
4. Olink Signature Q100 Workflows........................................................................................28
4.1 Workflow Overview.........................................................................................................28
4.2 Olink Target Workflows with the 96.96 and 48.48 chip...................................30
4.3 Olink Focus Workflow with the 24.192 chip..........................................................40
5. Troubleshooting..........................................................................................................................48
5.1 Run Instrument Checks..................................................................................................50
5.2 Export Instrument Check Logs.................................................................................... 51
5.3 Replace the Fuse............................................................................................................... 52
5.4 Shut Down the System .................................................................................................. 52
5.5 Start Up the System ........................................................................................................ 54
5.6 Shut Down and Turn Off the System ....................................................................... 54
5.7 Turn On and Start-Up the System ............................................................................ 55
5.8 Abort a Run in Progress................................................................................................. 56
5.9 Idle mode............................................................................................................................. 56
6. Preventive Maintenance, Decontamination, and Disposal....................................... 57
6.1 Preventive Maintenance................................................................................................ 57
6.2 Decontamination and Disposal.................................................................................. 59
7.
Technical support....................................................................................................................... 61
8. Revision history........................................................................................................................... 62
9. Appendix: Related Documents............................................................................................. 63
10. Appendix: Safety........................................................................................................................64
10.1 General Safety...................................................................................................................64
10.2 Instrument Safety.............................................................................................................64
2
1. About This User Manual
This user manual describes how to use the Olink&reg; Signature Q100 system, including the instrument and the system
software. For complete instrument specifications and unpacking/installation instructions, see Olink Signature Q100 Site
Requirements.
IMPORTANT: Before using the Olink Signature Q100 system, read and understand the safety guidelines in this
document. Failure to follow these guidelines may result in undesirable effects, injury to personnel, and damage
to the instrument or property.
For complete safety information, see “10. Appendix: Safety”.
1.1
Safety Alert Conventions
Olink documentation uses specific safety alert conventions for presenting information that may require your attention.
Refer to Table 1 and Table 2 for safety alert conventions for chemicals and instruments respectively.
1.1.1
Safety Alerts for Chemicals
For hazards associated with chemicals, this document follows the United Nations Globally Harmonized System of
Classification and Labelling of Chemicals (GHS) and uses indicators that include a pictogram and a signal word that
indicates the severity level:
Table 1.
Safety alerts for chemicals
Indicator
Description
Pictogram (see example) consisting of a symbol on a white background within a red
diamond-shaped frame. Refer to the individual safety data sheet (SDS) for the applicable
pictograms and hazards of the chemicals used.
DANGER
Signal word that indicates more severe hazards.
WARNING
Signal word that indicates less severe hazards.
1.1.2
Safety Alerts for Instruments
For hazards associated with instruments, this document uses indicators that include a pictogram and signal words that
indicate the severity level:
Table 2.
Safety alerts for instruments
Indicator
Description
Pictogram (see example) consisting of a symbol on a white background within a black
triangle-shaped frame. Refer to the instrument user guide for the applicable pictograms
and hazards of the instrument usage.
DANGER
Signal word that indicates an imminent hazard that will result in severe injury or death if
not avoided.
WARNING
Signal word that indicates a potentially hazardous situation that could result in serious
injury or death if not avoided.
CAUTION
Signal word that indicates a potentially hazardous situation that could result in minor or
moderate personal injury if not avoided.
IMPORTANT
Signal word that indicates information necessary for proper use of products or successful
outcome of experiments.
3
1.2
Safety Data Sheets
Read and understand the SDSs before handling chemicals. To obtain SDSs for chemicals ordered from Olink, either alone
or as part of this kit, go to olink.com and search for the SDS using either the product name or the part number.
Some chemicals referred to in this user guide may not have been provided with your system. Obtain the SDSs for
chemicals supplied by other manufacturers from those manufacturers.
4
2. System Overview
The Olink Signature Q100 system includes optical, thermal cycling, and software components necessary to perform
protein expression analysis using proximity extension assay technology.
The Olink Signature Q100 system provides throughput for proteomics that is higher by orders of magnitude than
conventional platforms using Integrated Fluidic Circuits (Chip; from now on referred to as chips)—nanofluidic circuits
containing fluidic networks that automatically combine sets of samples with sets of primers. This innovative solution for
real-time PCR provides experiment densities far beyond what is possible with microplate platforms, significantly reducing
the number of liquid‑handling steps and volumes per reaction.
2.1
Components of Olink Signature Q100
The Olink Signature Q100 system has a built-in computer that controls instrument operation and monitors its
performance. The onboard computer is operated only through the touchscreen.
Touchscreen
Drawer (retracted)
USB 3.0 port
Figure 1.
Power button
Olink Signature Q100 front view
NOTE: The port on the front of the instrument supports USB 3.0. We recommend using a USB drive compatible
with USB 2.0 or higher.
Olink Signature Q100 side view
5
POWERED BY
FLUIDIGM
Compressed air input
(optional)
HDMI
LAN
USB
COMPRESSION AIR INLET
(OPTIONAL)
70–90 PSI ONLY
AC 100–240V
50–60Hz, 8A
USB
HDMI port
Ethernet port
Main power switch
Fuse
Power socket
USB 2.0 ports
Figure 2.
Olink Signature Q100 rear view
2.2
Olink Signature Q100 Interface Plates
The Olink Signature Q100 interface plates are specific to the type of chip you are using. Store interface plates in the
storage container when not in use. The 96.96 Interface Plate comes with the Olink Signature Q100 system.
Component
Label Color
Supported chip
Olink Signature Q100 Interface Plate, 96.96
(96010)
Green
Olink 96.96 chip for Protein Expression
Olink Signature Q100 Interface Plate, 48.48
(96011)
Blue
Olink 48.48 chip for Protein Expression
Olink Signature Q100 Interface Plate, 24.192
(96012)
Red
Olink 24.192 chip for Protein Expression
NOTE: Each interface plate can be purchased individually from Olink.
Figure 3.
Olink Signature Q100 interface plates
2.3
Supported Chips
Olink Signature Q100 supports the following chips:
6
•
Olink 96.96 chip for Protein Expression (95007)
•
Olink 48.48 chip for Protein Expression (93007)
•
Olink 24.192 chip for Protein Expression (99007)
2.4
Supported Panels
Olink Signature Q100 supports the use of the following panels:
•
Olink&reg; Target 96 panels
•
Olink&reg; Target 48 panels
•
Olink&reg; Focus panels
2.5
Instrument Specifications
Refer to the Olink Signature Q100 Site Requirements Guide for instrument specifications and the recommended
environmental conditions.
2.6
Air Options
The Olink Signature Q100 system uses compressed air to pressurize fluids and control channels in the chip. The instrument
contains an internal compressor that draws in ambient air by default. Optionally connect it to an in-house air source.
NOTE: Use a clean, dry air (CDA) system whenever possible to help prevent corrosion within the pneumatic
system.
To use in-house compressed air instead of running the internal compressor, attach 4 mm tubing into the compressed air
inlet on the rear of the system and regulate the incoming air to 80–90 psi or 5.5–6.2 bar of CDA.
POWERED BY
FLUIDIGM
Compressed air input
(optional)
HDMI
LAN
AC 100–240V
50–60Hz, 8A
USB
USB
COMPRESSION AIR INLET
(OPTIONAL)
70–90 PSI ONLY
Figure 4.
Compressed air inlet
2.7
Ethernet Option
If you connect the Olink Signature Q100 instrument to your network, you can use domain authentication to manage user
accounts, import data directly from the instrument using the NPX Signature software, or enable remote technical support
through ethernet. For more information about domain authentication, “3.1.12 Enable Domain Authentication”.
NOTE: To enable NPX Signature remote data functions for the Q100 instrument, ensure that the instrument is
connected to the network and that TCP port 8085 (enabled by default) is not blocked.
NOTE: For information about importing run data in NPX Signature software, see the Olink NPX Signature User
Manual.
7
POWERED BY
FLUIDIGM
LAN
HDMI
Ethernet port
AC 100–240V
50–60Hz, 8A
USB
USB
COMPRESSION AIR INLET
(OPTIONAL)
70–90 PSI ONLY
Figure 5.
Ethernet port
2.7.1
Network Requirements
When adding Signature Q100 to network, it is necessary to add it to a secured segmented network that cannot access
internet. Olink will not take any responsibilities for the risks associated with networking of the instrument.
To connect the Signature Instrument to the network the following requirements must be met:
•
A DHCP enabled network with a dynamic IP-address allocation.
•
The Instrument must be prevented from reaching the Internet. (See Network Segmentation)
•
Communication attempts to the Instrument must allow TCP port: 8085
2.7.2
Network Segmentation
2.7.2.1
Introduction
To handle any security issues in the Local Network and making sure that the risk to the connected equipment is as small as
possible Network Segmentation is an important key factor. By segmenting the network, the following advantages can be
reached:
•
Damage control and limitation in case of an incident via the smaller attack surface. A smaller subset of network devices
is within the same scope.
•
Improved access control for external and internal network security.
– In this specific case the Signature Instrument should not reach the Internet and receive updates or be exposed to
threats.
•
Reducing the attack plane and scope of compliance requirements related auditing
•
Improved performance with less congestion on network traffic
•
Better analytics around network monitoring, network access and network devices
•
Endpoint device protection, especially important as IoT devices become more common
Network segmentation can boost your overall security policy by limiting access privileges to those who need it, protecting
the network from widespread cyberattacks and enabling better network performance by reducing the number of users in
specific zones.
2.7.2.2
How to implement Network Segmentation
This is heavily dependent on the flavor of Network Equipment used, and how to specifically implement segmentation can
only be answered when this is known. There are, however, a few basic technologies that is common for most solutions.
8
•
Firewall/router
– A firewall/router is used to handle multiple networks, and these can also be controlled through Access Rules. With
Access Rules we can prevent certain networks to reach resources they should not as well as protecting the network
resources from being reached from untrusted source. A capable firewall is also able to do much more, like web
filtering, intrusion prevention, antispam protection and preventing virus attacks.
o In a Network Segmentation Scenario, a VLAN or a separate LAN for the instrument should be created. A VLAN
(Virtual Local Area Network) has the advantage to use the same switches as other network segments – thus
reducing cost and space.
•
Switch
– A Network Switch is another key component and utilizing VLANs can be used to segment the network. By setting
specific VLANs on different ports the switch can be segmented, and the different networks can be isolated.
Together with the Firewall, traffic between the networks can be established if the scenario requires it.
2.8
Regulatory Compliance
The following directives and harmonized standards were used to evaluate the safety and performance of the Olink
Signature Q100 system:
2.8.1 General Regulations and Requirements
•
2014/35/EU European Parliament Low Voltage Directive
•
2014/30/EU European Parliament Directive: Electromagnetic Compatibility
•
2011/65/EU Restrictions on the use of certain hazardous substances in Electrical and Electronic Equipment (RoHS)
•
2012/19/EU Waste Electrical &amp; Electronic Equipment (WEEE)
•
EU Registration, Evaluation, Authorization, and Restriction of Chemicals (REACH), Regulation (EC) No 1907/2006
2.8.2 Harmonized Standards
•
EN 61326-1
•
IEC 61010-1 (including EN, CAN/CSA22.2, UL national deviations)
•
IEC 61010-2-010
•
IEC 61010-2-081 (including CAN/CSA22.2 national deviations)
2.8.3 Conformity Symbols on the Instrument
The instrument has the following conformity markings:
Conformity mark
Description
Indicates conformity with safety requirements for Canada and the United States.
Indicates conformity with European Union requirements for safety and
electromagnetic compatibility.
9
3. Olink Signature Q100
Operation
3.1
Olink Signature Q100 System Software
3.1.1
Unlock the Touchscreen
Swipe
toward the unlock icon to unlock the touchscreen.
3.1.2
Log In to the Olink Signature Q100 System
If required, enter your username and password to log in to the system.
10
After logging in, you have the following options:
•
Home screen: Start a new run.
•
History screen: View run logs for previous runs, export run data, and delete runs.
•
Settings screen:
– System: View system information (GUI version, IP address), enable or disable user authentication at login, change
the length of time before the screen locks, change the scheduled time for the automatic periodic instrument checks
to run, change the time settings for the system.
– User Accounts: Create users, change user permissions, disable or delete user accounts, and reset user passwords.
To access the User Accounts settings, you must have an account that has Manager permissions (see section &quot;3.2
User Accounts&quot;).
– Instrument Check: Run instrument checks on the Olink Signature Q100 instrument and export instrument logs.
For more information, see section “5.1 Run Instrument Checks”.
– Maintenance: The Maintenance settings can only be accessed by service personnel.
3.1.3
Start a New Run
To start a new run, tap the appropriate format for your chip type (Target 96, Target 48, or Focus). See “4. Olink Signature
Q100 Workflows” for instructions about specific workflows.
•
For the Target 96 and Target 48 runs, see “4.2 Olink Target Workflows with the 96.96 and 48.48 chip”.
•
For the Focus run, see “4.3 Olink Focus Workflow with the 24.192 chip”.
11
3.1.4
Onscreen Help
Olink Signature Q100 includes an on-screen help system. During the process steps of a run, tap
that contains instructions for how to perform the tasks required for that step of the workflow.
To close the on-screen help window, tap
3.1.5
in the top right corner.
View Run Logs and Export Data
Tap History to
12
•
view run details for previous runs
•
export run data
•
export comprehensive logs
•
delete run logs. Runs that have been exported show a checkmark (
) in the right column.
to open a new window
3.1.5.1
Export Data from a Run
1. Insert a USB drive into the USB port on the front of the instrument.
2. Identify the desired run log in the History screen.
3. Select and export the run files:
– Tap
or
in the Export column to select a run, then tap
,
or
– Tap a run to view the run details screen, then tap Export.
The files are copied to the USB drive. A Success message is displayed when the export is complete.
If there was a problem with the run, Technical Support might request the raw data files for that run. Insert a USB drive
into the USB port on the instrument’s front, then tap Service Export to export the raw data. Send the files to Technical
Support.
NOTE: The service export files can be up to 2 or 3 GB. The percent completed is displayed on the screen while
the files are copied to the USB drive.
13
3.1.5.2
Export Data from Multiple Runs
1. Insert a USB drive into the USB port on the front of the instrument.
2. In the History screen, tap
3. Tap
or
in the Export column to select each run you want to export.
(Export).
The files are copied to the USB drive. A Success message is displayed when the export is complete.
3.1.6 Delete Run Files
After you export run files, you can delete them from the instrument to free up storage.
The hard drive of the system can hold approximately 350 runs. The system displays a message when you have 15%
capacity remaining.
1. In the run details screen, tap
14
.
2. Tap Yes, delete to delete the selected run folder. If you do not want to delete the run, tap No. A Success message is
displayed when the deletion is complete.
3.1.7
Delete Multiple Run Files
You can delete the already exported run files. In the History screen, exported runs show a checkmark (
column.
) in the right
NOTE: Run files must be exported before they are deleted.
1. Go to the History screen.
2. Tap
or
in the Export column to select each run you want to delete, then tap
.
3. Tap Yes, delete to delete the selected run folders. If you do not want to delete the runs, tap No. A Success message is
displayed when the deletion is complete.
15
3.1.8 Change System Time Settings
1. Tap Settings &gt; System, then tap
(Edit) next to Time Settings.
2. Change the time zone and the date and time, then tap OK.
16
3. To view the time in 24-hour format, select the 24 Hour Format checkbox.
4. Tap Save Changes. Tap Yes to update the system settings. If you do not want to keep your changes, tap Cancel.
17
3.1.9
Change the Periodic Instrument Check Scheduled Time
1. Tap Settings &gt; System, then tap
(Edit) next to Periodic Instrument Check.
2. Change the time of day you want the automated instrument checks to occur, then tap Save Changes.
NOTE: The time interval for the automated checks is 30 days and cannot be changed.
3. Tap Yes to update the system settings. Tap Cancel if you do not want to keep your changes.
For information about running instrument checks manually, see “5.1 Run Instrument Checks”.
3.1.10 Change the Screen Lock Timeout
1. Tap Settings &gt; System, then tap
(Edit) next to Screen Lock Timeout.
Choose the amount of time you want your screen to stay on after the last user touches it, then tap Save Changes. If you do
not want the screen to lock, clear the Enabled checkbox.
2. Tap Yes to update the system settings. If you do not want to keep your changes, tap Cancel.
18
NOTE: To lock the screen before the specified time has elapsed, swipe the
top right corner of the screen.
button toward the lock icon in the
3.1.11 Enable or Disable User Authentication
You can require users to log in to the instrument with a password.
IMPORTANT: To enable or disable user authentication, you must log in with an account with Manager
permissions.
1. Tap Settings &gt; System, then tap
(Edit) next to Authentication.
2. Enter a Manager username and password (contact Olink Technical Support if you are the instruments Manager and
you don't have access to username and password.).
3. Tap to enable or disable the Require Authentication checkbox, then tap Save Changes.
4. Tap Yes to update the system settings. If you do not want to keep your changes, tap Cancel.
After enabling authentication, you can access the User Accounts settings and add or remove users and change user
information. For more information about user accounts, see “3.2 User Accounts”.
3.1.12 Enable Domain Authentication
When user authentication is enabled, you have the option to use your lab’s domain authentication system to manage user
accounts.
To enable domain authentication for your instrument:
1. Create the following user groups in the lab’s Windows Active Directory:
– Olink Instrument Operator
– Olink Instrument Manager
2. Add users to the appropriate user groups.
3. Connect an Ethernet network cable to the port on the back of the instrument.
19
4. Plug the instrument Ethernet cable into an active port that can communicate with the lab’s active directory.
5. Enable authentication on the instrument:
a. Tap Settings &gt; System, then tap
(Edit) next to Authentication.
b. Tap to enable the Require Authentication checkbox, tap Save Changes, then tap Yes to update the system
settings.
After enabling authentication you can log in to the instrument using your Windows Domain credentials.
3.2
User Accounts
After enabling authentication, you can access the User Accounts settings and add or remove users and change user
information.
NOTE: To obtain or reset the Manager password, contact Technical Support to request a recovery key.
The Olink Signature Q100 system has three types of user accounts:
Account Type
Description
Manager
Managers can:
• Log into the system
• Start new runs and abort runs started by any user
• Add new users
• Change other users’ settings, including permissions and password
Operator
Operators can:
• Log into the system
• Start new runs and abort runs that they started
Operators cannot:
• Operators cannot abort a run started by a different user.
• Delete user accounts
Disabled
3.2.1
A user with a Disabled account is no longer allowed to log in, but the account is still in
the system for auditing purposes.
Add a New User
IMPORTANT: To add a new user, enable user authentication, and log in with an account with Manager
permissions.
1. Log in to the Olink Signature Q100 system as a Manager.
2. Tap Settings &gt; User Accounts.
3. Tap
20
(New User).
4. Enter the information for the new user:
– Username: the name used to log in to the system.
– Display name: the name displayed in the History screen and the run information.
– Password: the password used to log in to the system.
– Permissions: Select Manager or Operator.
5. Tap Save.
3.2.2 Disable a User Account
A user with a Disabled account is no longer allowed to log in, but the account is still in the system for auditing purposes.
IMPORTANT: To disable a user account, you must log in with an account that has Manager permissions.
1. Log in to the Olink Signature Q100 system as a Manager.
2. Tap Settings &gt; User Accounts.
3. Tap a user to open the details screen.
4. For Permissions, select Disabled.
5. Tap Save.
21
3.2.3 Delete a User
IMPORTANT: To delete a user, you must log in with an account that has Manager permissions.
1. Log in to the Olink Signature Q100 system as a Manager.
2. Tap Settings &gt; User Accounts.
3. Tap a user to open the details screen.
4. Tap
.
5. Tap Yes, sure to delete the user account. If you do not want to delete the user account, tap No.
3.2.4 Change Your Password
When Authentication is enabled, a user with an Operator account can change their password.
1. Log in to the Olink Signature Q100 system.
2. Tap Settings &gt; User Accounts.
3. Tap your username to open the Details screen.
4. Tap Change Password, then follow the prompts to change the password.
22
3.3
Best Practices
IMPORTANT: Read and understand the safety information in “10. Appendix: Safety”.
For the overall success of the experiment, we recommend the following best practices:
3.3.1
Chip and Control Line Fluid Handling
•
Use the chip within 24 hr of opening the foil envelope.
•
Inspect the chip for any signs of visible damage before use. Ensure that the barcode labeli is intact,
and the chip surfaces are clear of particulates.
•
To avoid loss of control line fluid, do not evacuate air from a syringe before injecting control line fluid.
•
Avoid bending the syringe tip of the control line fluid.
•
Be careful when removing the syringe cap of the control line fluid to prevent drips.
•
Before removing the syringe from the accumulator, ensure that all of the control line fluidand air are purged from the
syringe. This is to avoid dripping fluid on the chip surface. Residual control line fluid or air in the syringe tip is normal.
•
Control line fluid that occurs on the chip exterior or inlets makes the chip unusable. If this occurs, use a new chip.
•
During use, take care to avoid introducing particulates, reagents, and fluids to the chip surface.
3.3.2 Bubble Prevention
•
Vortex the 96-well primer and sample plates gently (low speed) but thoroughly (at least 5 sec) to allow all reagents to
mix homogeneously.
•
After vortexing the primer and sample mixes, centrifuge them to collect all compounds at the bottoms of the wells
before pipetting into the chip inlets. Failure to do so may result in a decrease in data quality.
•
Check the source plates or tubes for bubbles before pipetting.
•
Check pipette tips for air gaps while pipetting.
•
Pipet reagents slowly and carefully to transfer entire volumes, to minimize bubbles, and ensure accurate and precise
delivery of liquids.
•
To avoid creating bubbles in the chip inlets, pipet vertically and directly into the bottom of the inlet, as shown, and do
not go past the first stop on the pipette. If a bubble occurs, ensure that it floats to the top of the inlet.
Pipetting into bottom of inlet
•
If necessary, remove medium-to-large bubbles from a chip inlet by popping them with a new pipette tip or by
removing the contents of the inlet by pipette and then carefully re- pipetting the contents into the inlet.
•
Place the chip on a black surface to reduce glare and make it easier to see the liquid in each inlet when looking for
bubbles.
23
4. Olink Signature Q100
Workflows
4.1
Workflow Overview
Step 1
Step 2
Step 3
Step 4
Step 5
Prepare primers
and samples.
Target 48 and 96:
Prepare the chip
and load primers
and samples.
Focus: Load primers
and samples.
Target 48 and 96:
Run the chip and
collect data on
Olink Signature
Q100.
Focus: Prepare
and run the chip
and collect data
on Olink Signature
Q100.
Transfer the run
data using a USB
drive or directly
import data from
the NPX Signature
software.
Analyze data
using the NPX
Signature
software.
Step Descriptions for 48.48 and 96.96 chips
Workflow Step
1
Prepare the primer and sample mixes.
NOTE: Do not pipet the samples into the chip at this stage.
2
Inject control line fluid into the accumulators on the chip.
NOTE: Remember to remove the protective film at this stage.
Prepare the chip on Signature Q100. This primes the chip ready for use.
Remove the chip from the instrument and pipet each final sample and primer mix into the chip.
3
Run the chip and collect data on Olink Signature Q100.
4
Transfer the run data using a USB drive or directly import data from the NPX Signature software.
5
Analyze data using the NPX Signature software.
Step Descriptions for 24.192 chips
Workflow Step
1
Prepare the primer and sample mixes.
NOTE: Do not pipet the samples into the chip at this stage.
2
Inject control line fluid into the top accumulator on the chip.
NOTE: Remember to remove the protective film at this stage.
Pipet each final sample and primer mix and the control reagents into the chip.
3
Run the chip and collect data on Olink Signature Q100.
NOTE: Preparing and running the 24.192 chip occurs in 1 step.
24
4
Transfer the run data using a USB drive or directly import data from the NPX Signature software.
5
Analyze data using the NPX Signature software.
4.2
Olink Target Workflows with the 96.96 and 48.48 chip
NOTE: Prepare your primers and samples as described in the Olink Target 96 User Manual (Olink 0935) or the
Olink Target 48 User Manual (Olink 1141).
4.2.1 Prepare the 96.96 or 48.48 chip
Before pipetting primers and samples into the chip, you must prepare the chip with control line fluid.
4.2.1.1
Inject Control Line Fluid into the Accumulators
IMPORTANT: When injecting control line fluid:
•
Follow the best practices for handling chips and control line fluid, see “3.3.1 Chip and Control Line Fluid
Handling”.
•
Only use Olink&reg; Control Line Fluid for 96.96 chip syringes or Olink Control Line Fluid for 48.48 chip syringes.
Control line fluid syringes are prefilled according to a specific chip type.
1. Remove the control line fluid syringes from the packaging and the chip from the box and foil envelope.
IMPORTANT: To avoid loss of control line fluid: Do not evacuate air from a syringe before injecting control line
fluid. (Step 4).
Optional step to ensure proper chip function:
2. Actuate the check valves:
a. Place the chip on a flat surface.
b. Use the syringe with the shipping cap to actuate the check valve in each accumulator with gentle pressure. Ensure
that the poppet can move freely up and down (Figure 7).
96.96 chip
48.48 chip
Cap
Check valve
Figure 6.
Cap
Check valve
Actuating the check valve in the top accumulator on the Olink 96.96 chip and the Olink 48.48 chip
3. Hold the syringe firmly in one hand with the tip facing up and away from the chip, pull back on the plunger slightly to
create negative pressure, and remove the shipping cap with the other hand. Be careful when removing the syringe cap
of the control line fluid to prevent drips.
4. Holding the chip at a 45&deg; angle, insert the syringe tip into an accumulator (Figure 7).
IMPORTANT:
•
Avoid bending the syringe tip. Be careful when removing the syringe cap to prevent drips.
•
Control line fluid on the chip exterior or inlets makes the chip unusable. If this occurs, use a new chip.
25
96.96 chip
Figure 7.
48.48 chip
Inserting the syringe into an accumulator
5. Insert the syringe tip into one of the spaces between the arms of the X at the top of the valve and then press down
gently to move the black O-ring to the side. Visually confirm that the O-ring has moved (Figure 8).
6. Release the control line fluid:
a. Press the syringe plunger to release the control line fluid into the accumulator while maintaining the 45&deg; angle to
allow the liquid to flow away from the O‑ring.
b. Slowly inject the control line fluid by pushing down on the syringe plunger. The control line fluid flows into the
accumulator through the open check valve. Use the entire contents of the syringe.
c. After fully depressing the plunger, wait approximately 5 sec before withdrawing the syringe. Before removing the
syringe from the accumulator, ensure that all the control line fluid and air are purged from the syringe. This is to
avoid dripping fluid on the chip surface. Residual control line fluid or air in the pipette tip is normal.
96.96 chip
Figure 8.
48.48 chip
Syringe tip inserted into
one of the saces between
the arms of the &quot;X&quot;.
Syringe tip inserted into
one of the saces between
the arms of the &quot;X&quot;.
Move the O-ring to the side.
Move the O-ring to the side.
Injecting control line fluid into the chip
7. Check to ensure that the O‑ring returns to its normal position after the syringe is removed.
8. Pull the protective film down and away from the bottom of the chip. Discard the film.
9. Place the appropriate (chip-specific) Olink Signature Q100 interface plate over the chip. Align the barcoded edges.
IMPORTANT: Load the chip within 60 min of completing chip preparation.
26
4.2.1.2
Prepare the Target 48 or Target 96 chip on Olink Signature Q100
NOTE: If you see the Lock icon, swipe it to unlock it.
1. If required, log into the Olink Signature Q100 instrument.
2. On the Home screen, select the format that matches the chip you are using:
– Tap Target 96 for the 96.96 chip.
– Tap Target 48 for the 48.48 chip.
NOTE: For more information about the task displayed on the screen, tap
.
3. After you tap Target 96 or Target 48, the drawer opens.
4. Place the chip containing control line fluid and the interface plate on the drawer. Align the notched corner of the chip
to the notch on the drawer and face the barcoded edges of the chip and interface plate forward.
NOTE: Make sure that the protective film has been removed.
Barcodes on
interface plate
and Chip
Notched corner in
chip and drawer
5. Tap Close Drawer. The system scans the chip barcode, closes the drawer, and then displays the Ready to start chip
preparation screen.
27
NOTE: If you need to eject the chip, tap
(Eject) at the bottom of the screen to return to the Insert a chip with
control line fluid into the instrument screen.
6. Tap Start. Preparation begins and displays the time remaining.
NOTE: If you need to abort the preparation, tap
Progress” for more information.
28
(Abort) at the bottom of the screen. See “5.8 Abort a Run in
7. When preparation is complete, tap
(Eject) in the bottom right corner to open the drawer.
8. Remove the chip and interface plate from the instrument, then tap Close Drawer.
IMPORTANT: Do not leave the drawer open while you are pipetting samples and primers into the chip.
29
4.2.2 Pipet Primers and Samples into the 96.96 or 48.48 chip
•
IMPORTANT:
Vortex all primers, samples, and control reagents thoroughly, then centrifuge them to collect contents before
removing the plate seals and pipetting the mixes into the chip inlets. Failure to do so may result in a decrease in
data quality.
•
While pipetting, do not go past the first stop on the pipette. Doing so may introduce air bubbles into inlets.
•
Follow the best practices for preventing bubbles, see “3.3.2 Bubble Prevention”.
Refer to Figure 9 or Figure 10 as appropriate when pipetting the final sample and primer mixes into the 96.96 or 48.48
chip. Note the orientation of the A1 corner. The barcoded edge is on the left side.
NOTE: Olink recommends using the reverse pipetting method when pipetting the sample and primer mixes. See
the Olink&reg; Target 96 User Manual (Olink 0935) or the Olink&reg; Target 48 User Manual (Olink 1141).
1. Remove the interface plate from the prepared chip.
2. Pipet 5 μL of each final sample mix into the respective sample inlets on the chip.
3. Pipet 5 μL of each final primer mix into the respective primer inlets on the chip.
4. Replace the interface plate over the chip, aligning the barcoded edges.
5. Use clear tape to remove any dust particles or debris from the chip surface, if necessary.
IMPORTANT: Run the chip on Olink Signature Q100 within 60 min of pipetting the samples and primers.
Sample Plate 2
Primer Plate
Primers
Figure 9.
30
Pipetting map for 96.96 chip
Samples
Primer Plate
Sample Plate
Primers
Samples
Figure 10. Pipetting map for the 48.48 chip
4.2.3 Run the 96.96 or 48.48 chip on Olink Signature Q100
NOTE: If you see the Lock icon, swipe it to unlock the system.
1. If required, login to the Olink Signature Q100 instrument.
2. On the Pipette samples and primers onto the chip screen, tap Open Drawer.
NOTE: Olink Target 48 chip screen shown.
3. Make sure that the Inteface plate is placed properly on the chip and in level with the drawer. Align the notched corner
of the chip with the notch on the drawer and face the barcoded edges of the chip and interface plate forward.
31
NOTE: Make sure that the protective film has been removed.
Barcodes on
interface plate
and Chip
Notched corner in
chip and drawer
4. Tap Close Drawer. The system scans the chip and interface plate barcodes, then closes the drawer.
5. On the Add run details screen, confirm the Chip ID, then enter any of the following details for the run:
– Run Name (the default name is the Chip ID). Max number of characters is 100
– Run Notes
NOTE: If you need to eject the chip, tap
(Eject) at the bottom of the screen. The software returns to the
Insert the chip containing samples and primers into the instrument screen.
6. Tap Start Run. The run begins, and the screen displays the time remaining.
NOTE: If you need to abort the run, tap
for more information.
32
(Abort) at the bottom of the screen. See “5.8 Abort a Run in Progress”
7. When the run is complete, tap Finish.
8. (Optional) Export your data:
a. Insert a USB drive into the USB port on the front of the instrument.
b. On the Run Successfully Completed screen, tap
(Export Data) to copy the run data to the USB drive.
NOTE: You can export the data files later from the History screen. See “3.1.5 View Run Logs and Export Data” for
more information.
33
9. Tap Open Drawer, then remove the chip and interface plate from the instrument.
10. Tap Close Drawer.
11. Return the interface plate to the storage container. Discard the used chip.
12. Analyze the exported data using the NPX Signature software.
NOTE: You can export data onto a USB drive or access it using ethernet (see “2.7 Ethernet Option”).
34
4.3
Olink Focus Workflow with the 24.192 chip
NOTE: Prepare your primers and samples as described in the separate instructions for the specific panel.
4.3.1 Prepare the 24.192 chip
Before pipetting assays and samples into the 24.192 chip, prepare the chip with control line fluid.
IMPORTANT: When injecting control line fluid:
•
Follow the best practices for handling chips and control line fluid, see “3.3.1 Chip and Control Line Fluid Handling”.
•
Only use an Olink Control Line Fluid for a 24.192 chip syringe. Control line fluid syringes are prefilled according
to a specific chip type.
1. Remove the control line fluid syringe from the packaging and the chip from the box and foil envelope.
IMPORTANT: Do not evacuate air from the syringe before injecting control line fluid (Step 4).
Optional step to ensure proper chip function:
2. Actuate the top check valve:
a. Place the chip on a flat surface.
b. Use the syringe with the shipping cap to actuate the check valve in the top accumulator with gentle pressure.
Ensure that the poppet can move freely up and down (Figure 11).
Notched
corner
Top accumulator
Cap
Check valve
Bottom accumulator
DO NOT USE!
Figure 11. Actuating the check valve in the top accumulator on the 24.192 chip
3. Hold the syringe firmly in 1 hand with the tip facing up and away from the chip, pull back on the plunger slightly to
create negative pressure, and remove the shipping cap with the other hand. Be careful when removing the syringe cap
of the control line fluid to prevent drips.
4. Holding the chip at a 45&deg; angle, insert the syringe tip into the top accumulator (Figure 12).
IMPORTANT:
•
Avoid bending the syringe tip. Be careful when removing the syringe cap to prevent drips.
•
Avoid getting control line fluid on the exterior of the chip or in the inlets because this makes the chip unusable.
If this occurs, use a new chip.
35
Notched A1 corner
Figure 12. Inserting the syringe into the accumulator. Note the position of the notched A1 corner.
5. Use the syringe tip to press down gently on the black O-ring to move it (Figure 13). Visually confirm that the O-ring has
moved.
Syringe tip
Press down on the O-ring
Figure 13. Injecting control line fluid into the chip
6. Release the control line fluid:
a. Press the syringe plunger to release the control line fluid into the accumulator while maintaining the 45&deg; angle to
allow the liquid to flow away from the O‑ring.
b. Slowly inject the control line fluid by pushing down on the syringe plunger. The control line fluid flows into the
accumulator through the open check valve. Use the entire contents of the syringe.
c. After fully depressing the plunger, wait approximately 5 sec before withdrawing the syringe. Before removing the
syringe from the accumulator, ensure that all the control line fluid and air are purged from the syringe. This is to
avoid dripping fluid on the chip surface. Residual control line fluid or air in the pipette tip is normal.
7. Make sure that the O‑ring returns to its normal position after the syringe is removed.
8. Pull the protective film down and away from the bottom of the chip. Discard the film.
4.3.2 Pipet Primers, Samples, and Control Reagents into the 24.192 chip
IMPORTANT:
•
Vortex all primers, samples, and control reagents thoroughly, then centrifuge them to collect contents before
removing the plate seals and pipetting the mixes into the chip inlets. Failure to do so may result in a decrease in
data quality.
•
While pipetting, do not go past the first stop on the pipette. Doing so may introduce airbubbles into inlets.
•
Follow the best practices for preventing bubbles, see “3.3.2 Bubble Prevention”.
Refer to Figure 14 when pipetting the final sample and primer mixes and Figure 15 when pipetting the control reagents
(Actuation Fluid, Pressure Fluid) into the chip. Note the orientation of the A1 corner. The barcoded edge is on the left side.
NOTE: Olink recommends using the reverse pipetting method when pipetting the sample and primer mixes. See
the Olink&reg; Target 96 User Manual (Olink 0935) or the Olink&reg; Target 48 User Manual (Olink 1141).
36
1. Pipet 3 μL of each sample mix into the respective sample inlets on the chip.
2. Pipet 3 μL of each primer mix into the respective primer inlets on the chip.
3. Pipet 150 μL of Actuation Fluid (100-6250) into the P1 reservoir (
) on the chip.
4. Pipet 150 μL of Pressure Fluid (100-6249) into each of the P2 and P3 reservoirs (
) on the chip.
5. Pipet 20 μL of Pressure Fluid into each of the P4 and P5 inlets ( ) on the chip.
6. Place the Olink Signature Q100 Interface Plate (24.192) over the chip, aligning the barcoded edges.
7. Use clear tape to remove any dust particles or debris from the chip surface, if necessary.
IMPORTANT: Run the chip on Olink Signature Q100 within 60 min of pipetting the samples, primers, and
fluids.
Sample Plate 2
Sample Plate 1
Primers
Primers
Samples
Samples
Primer Plate
Figure 14. Primer and sample pipetting map for the 24.192 chip
37
Actuation Fluid
P1
P4
P2
P5
P3
Pressure Fluid
Figure 15. Control reagents pipetting map for the 24.192 chip
4.3.3 Run the 24.192 chip on Olink Signature Q100
NOTE: If you see the Lock icon, swipe it to unlock the system.
1. If required, log in to the Olink Signature Q100 instrument.
2. On the Start a New Run screen, tap Focus.
NOTE: For more information about the task displayed on the screen, tap
.
3. On the Pipette samples, primers, and control reagents onto the chip screen, tap Open Drawer.
38
4. Make sure that the Inteface plate is placed properly on the chip and in level with the drawer. Align the notched corner
of the chip to the notch on the drawer and face the barcoded edges of the chip and interface plate forward.
NOTE: Make sure that the protective film has been removed.
Barcodes on
interface plate
and Chip
Notched corner in
chip and drawer
5. Tap Close Drawer. The system scans the chip barcode, then closes the drawer.
6. On the Add run details screen, confirm the Chip ID, then enter any of the following details for the run:
– Run Name (the default name is the Chip ID). Max number of characters is 100.
– Run Notes
NOTE: If you need to eject the chip, tap
(Eject) on the Add run details screen. The software returns to the
Insert the chip containing samples, primers and, control reagents into the instrument screen.
39
7. Tap Start Run. The run begins, and the screen displays the time remaining.
NOTE: If you need to abort the run, tap
for more information.
8. When the run is complete, tap Finish.
40
(Abort) at the bottom of the screen. See “5.8 Abort a Run in Progress”
9. (Optional) Export your data:
a. Insert a USB drive into the USB port on the front of the instrument.
b. On the Run Successfully Completed screen, tap
(Export Data) to copy the run data to the USB drive.
NOTE: You can export the data files later in the History screen. See “3.1.5 View Run Logs and Export Data” for
more information.
10. Tap Open Drawer, then remove the chip and interface plate from the instrument.
41
11. Tap Close Drawer.
12. Return the interface plate to the storage container. Discard the used chip.
13. Analyze the exported data using the NPX Signature software.
NOTE: You can export data onto a USB drive or access it using ethernet (see “2.7 Ethernet Option”).
42
5. Troubleshooting
Observation
Possible Cause
The system failed to power on. No AC power.
Recommended Action
Confirm that the power cable is
connected correctly to the power
socket and the instrument.
Confirm that the power socket has
power.
Confirm that the toggle switch at the
rear of the instrument is in the ON
position.
Initialization error.
Error during system self-check. Restart the system by turning off
the power switch at the rear of
the instrument, wait for at least 15
seconds, and then turning it on again.
If the system fails to recover, contact
Olink&reg;Technical Support.
No or low vacuum.
Dust on the bottom of the
chip or the top of the thermal
chuck.
Confirm that the protective film was
removed from the chip prior to loading
on the tray.
Clean the thermal chuck using a lintfree cloth or swabs and 70% ethanol or
70% isopropyl alcohol. See “6.1.2 Clean
the Thermal Cycler Chuck” for detailed
instructions.
Sometimes, it also helps to use clear
tape to remove dust from the thermal
chuck.
Clean the bottom of the chip using a
lint-free cloth and 70% ethanol or 70%
isopropyl alcohol.
After cleaning the thermal chuck and
chip, retry loading into the system. If
the system is still giving an Insufficient
vacuum error, contact Olink Technical
Support.
No or low vacuum.
Blocked port(s) of the
interface plate.
Clean the Interface plate to remove
dust, see &quot;6.1.3 Clean the Interface
Plate&quot;.
43
44
Observation
Possible Cause
Recommended Action
Run failure.
Various.
Run a comprehensive instrument check
(see “5.1 Run Instrument Checks”), then
contact Olink&reg; Technical Support.
Instrument is in Demo mode.
The instrument was left in
Demonstration mode by
mistake.
Contact Olink&reg; Technical Support to
change the instrument back into Run
mode.
Chip scan error.
Chip was loaded without its
Interface plate on.
Repeat loading with correct Interface
plate on top of the chip.
Drawer can not close properly
and makes noise.
Interface plate was not placed
properly on the chip.
Pull out the drawer and repeat loading
with Interface plate properly on top of
the chip.
5.1
Run Instrument Checks
You can run two types of instrument checks:
•
Comprehensive: Checks all components to make sure everything is working correctly (for example, opens and closes
valves, raises and lowers the camera, etc.). The comprehensive check takes 8–10 min to complete.
•
Quick: Checks that all components are connected and communicating properly. The quick check takes 5–8 min to
complete.
NOTE: If a chip is located in the instrument, it is not possible to execute the &quot;Run Instrument Checks&quot;.
Remove the chip before starting the check
1. On the touchscreen, tap Settings &gt; Instrument Check.
2. Tap Run Instrument Check.
3. In the Run Instrument Check window, tap the type of check you want to run.
4. Tap Start. When the test is complete, the new log file appears in the Instrument Logs list.
45
If the Instrument check fails, contact Olink&reg; Technical Support.
5.2
Export Instrument Check Logs
If Technical Support requests the instrument check logs, export the logs as follows:
1. Insert a USB drive into the USB port on the front of the instrument.
2. Tap
, select the date range for the logs you want to export, then click Export.
The logs are exported to the USB drive in a .blmg folder. A Success message is displayed when the export is complete.
3. Send the .blmg folder to Technical Support, as requested.
5.3
46
Replace the Fuse
No fuse replacement is required during installation. If the power fuse is faulty, then replace the fuse:
1. Toggle off the main power switch on the rear of the instrument, then unplug the power cord from the instrument.
2. Remove the fuse holder below the main power switch by pushing and releasing the fuse holder blocks.
Main power switch
Fuse holder blocks
FUSE
T8AL 250VAC
LAN
HDMI
POWERED BY
FLUIDIGM
AC 100–240V
50–60Hz, 8A
USB
USB
COMPRESSION AIR INLET
(OPTIONAL)
70–90 PSI ONLY
3. Replace the fuse (T8AL, 250 V AC, 5 &times; 20 mm) and reinstall the fuse holder. Follow the arrow marks in the fuse holder
to match the casing.
4. Plug the power cord into the instrument, then toggle on the main power switch.
5.4
Shut Down the System
If you need to shut down the system due to an unrecoverable error, use the instrument’s power button on the front.
Power button
1. Gently press and hold the power button until the touchscreen displays the following message:
2. Tap Yes, continue to proceed with system shutdown.
NOTE: If a run is in progress, the screen displays a message:
47
You can either abort the run and shut down or wait until the run is complete before trying to power down the system
again
3. Wait 10 sec. The screen goes dark, and the power button becomes red.
IMPORTANT: Wait at least 15 sec after shutting down the instrument before turning it back on.
5.5
Start Up the System
When the power button on the front of the instrument is red, gently press it to start up the system.
During startup, the instrument initializes all system components and software. It also performs a set of instrument checks
to ensure that the system components are working correctly. Initialization and the instrument checks take approximately
10 min to complete. Export the results from the Instrument Check screen under Settings (see “5.1 Run Instrument Checks”
for more information). The system can remain turned on when not in use.
NOTE: If not planned to be used, e.g., for a week, turn off Olink&reg; Signature Q100 to save power.
5.6
Shut Down and Turn Off the System
Shut down and turn off the system completely before unplugging it or moving it. It takes approximately 1 min for the
system to shut down.
IMPORTANT: Do not toggle off the main power switch without first shutting down the system.
1. Shut down the system using the power button on the front of the instrument.
48
Power button
a. Gently press and hold the power button until the touchscreen screen displays the following message:
b. Tap Yes, continue to proceed with system shutdown.
NOTE: If a run is in progress, the screen displays a message:
NOTE: You can either abort the run and shut down or wait until the run is complete before trying to power down
the system again.
c. Wait 10 sec while the screen goes dark. The power button becomes red.
2. Toggle off the main power switch on the rear panel of the instrument.
49
LAN
COMPRESSION AIR INLET
(OPTIONAL)
70–90 PSI ONLY
AC 100–240V
50–60Hz, 8A
USB
USB
Main power switch
HDMI
POWERED BY
FLUIDIGM
IMPORTANT: Wait at least 15 sec after turning off the instrument before turning it back on.
5.7
Turn On and Start-Up the System
When the system is turned off, turn it on by toggling on ( | ) the main power switch on the instrument’s rear panel.
The system starts up, and the instrument initializes all system components and software. It also performs a set of
instrument checks to ensure that the system components are working correctly. The system can remain turned on when
not in use.
NOTE: If not planned to be used, e.g., for a week, turn off Olink&reg; Signature Q100 to save power.
5.8
Abort a Run in Progress
IMPORTANT: Aborted runs cannot be restarted. Discard any chips from aborted runs.
If you need to abort a run in progress:
1. Tap
50
(Abort) at the bottom of the screen, then tap OK to end the run in progress.
2. Tap Open Drawer, then remove the chip and interface plate.
3. Tap Close Drawer to return to the Home screen.
5.9
Idle mode
The instrument goes into idle mode automatically when there is no user interaction for a long while.
Touch the screen to take the instrument out of its idle mode.
51
6. Preventive Maintenance,
Decontamination, and Disposal
6.1
Preventive Maintenance
Before using a cleaning or decontamination method other than those recommended by Olink, verify with Olink Technical
Support that the proposed method will not damage the equipment.
This section describes how to clean and maintain your Olink Signature Q100 system for optimal performance.
6.1.1
Preventive Maintenance Recommendations
For optimal performance of your Olink Signature Q100 system, we recommend:
•
Performing annual maintenance by a certified Olink service engineer.
•
Ordering a replacement interface plate from Olink when the plate approaches its life expectancy of 2,000 runs.
•
Only using replacement parts supplied by Olink.
6.1.2 Clean the Thermal Cycler Chuck
When instrument is in use, we recommend cleaning the thermal cycler chuck once a day.
1. Wait at least 5 minutes to allow the system to cool down after a completed run.
CAUTION: HOT SURFACE HAZARD. The thermal cycler chuck gets hot and can burn
your skin. Use caution when working near the chuck.
2. Tap Open Drawer.
3. Remove the chip and interface plate from the drawer. Store the interface plate and discard the used chip.
4. Moisten a lint-free cloth or soak lint-free swabs with 70% ethanol or 70% isopropyl alcohol.
WARNING: BIOHAZARD. If you are putting biohazardous material on the instrument,
use appropriate personal protective equipment and adhere to Biosafety in
Microbiological and Biomedical Laboratories (BMBL), a publication from the Centers
for Disease Control and Prevention, and to your lab’s safety protocol to limit biohazard
risks. If biohazardous materials are used, then label the equipment properly as a
biohazard. For more information, see the BMBL guidelines online at cdc.gov/biosafety/
publications/index.htm.
5. With the cloth or swabs, remove debris from the thermal cycler chuck by gently wiping towards the vacuum holes
according to the pattern of the chuck. Next, wipe along the perimeter of the chuck:
52
6.1.3 Clean the Interface Plate
We recommend visually checking the interface plate after each run. If dust particles or debris has accumulated, clean the
interface plate as follows:
1. Use a lint-free cloth and 70% ethanol or 70% isopropyl alcohol to gently wipe the bottom and top of the interface plate
to remove any debris:
2. Store the interface place in the storage container.
NOTE: If there is a sign of tears in the surface of the silicone surface it may need to be replaced, see &quot;6.1.1
Preventive Maintenance Recommendations&quot;.
6.1.4 Clean the Instrument and Touchscreen
•
Use a lint-free cloth and 70% ethanol or 70% isopropyl alcohol to gently wipe the top of the instrument drawer and the
instrument covers as needed.
•
Wipe down the touchscreen with a lint-free cloth as needed.
IMPORTANT:
•
Do not spray cleaning solution directly on the instrument or touchscreen, as it may penetrate the seams
around the screen and cause damage. Spray the cleaning solution on a lint-free cloth and then gently wipe the
instrument surface.
•
Do not use bleach to clean the instrument as it is corrosive to metal.
•
Before using a cleaning or decontamination method other than those recommended by Olink&reg;, verify with Olink
Technical Support that the proposed method will not damage the equipment.
53
6.1.5 Update the Software
1. Obtain the software installer (.pak file).
2. Extract the files to your local computer, then copy them to a USB drive.
3. Insert the USB drive into the USB port on the front of Olink Signature Q100.
4. On the instrument, go to the Settings screen, then tap UPGRADE.
NOTE: The Upgrade button is visible only if a USB drive containing the .pak file is inserted in the instrument.
5. Follow the prompts to complete the software update.
6.2
Decontamination and Disposal
WARNING: BIOHAZARD. When handling biohazardous materials or using
biohazardous material on the instrument or system, use appropriate personal
protective equipment. Adhere to Biosafety in Microbiological and Biomedical
Laboratories (BMBL), a publication from the Centers for Disease Control and
Prevention, and your lab’s safety protocol to limit biohazard risks. If biohazardous
materials are used, label the equipment properly as a biohazard. For more information,
see the BMBL guidelines online at cdc.gov/biosafety/publications/index.htm.
Ensure that the Olink Signature Q100 system is cleaned or decontaminated before servicing the equipment, removing it
from use, or transporting it for disposal. Refer to the instructions contained in this document and use only those materials
specified.
6.2.1 Biological Agents
1. Using a soft cloth, apply 70% ethyl alcohol or 70% isopropyl alcohol to all accessible surfaces.
2. Keep surfaces wet for at least 5 min and then wipe dry.
3. Repeat Steps 1 and 2 once.
4. Clean all decontaminated surfaces with a wet cloth to remove residual alcohol and wipe dry.
54
6.2.2 Hazardous Chemicals
IMPORTANT: Before use, ensure that alcohol is compatible with the chemicals used.
1. Using a soft cloth, apply 70% ethyl alcohol or 70% isopropyl alcohol to all accessible surfaces.
2. Keep surfaces wet for at least 5 min and then wipe dry.
3. Repeat Steps 1 and 2 once.
4. Clean all decontaminated surfaces with a wet cloth to remove residual alcohol and wipe dry.
6.2.3 Disposal of Products
Used chips and reagents should be handled and disposed of following federal, state, regional, and local laws for
hazardous waste management and disposal.
Do not dispose of this product in unsorted municipal waste. This equipment may contain
hazardous substances that could affect health and the environment. Use appropriate
take‑back systems when disposing of materials and equipment.
Learn more at olink.com.
55
7. Technical support
For technical support contact [email protected].
56
8. Revision history
Version
Date
Description
1.1
2022-01-25
Added text to section 2.7
Editorial changes
1.0
2021-11-11
New
57
9. Appendix: Related Documents
Go to https://www.olink.com/resources-support/document-download-center/ to download these related documents.
58
Title
Document Number
Olink&reg; Target 96 User Manual
0935
Olink&reg; Target 48 User Manual
1141
Olink&reg; Signature Q100 Site Requirements Guide
1170
Olink&reg; NPX Signature User Manual
1173
Olink&reg; Signature Q100 Installation Guide
1171
Olink&reg; Signature Q100 - A benchtop system for protein biomarker analysis
1164
10. Appendix: Safety
10.1 General Safety
In addition to your site-specific safety requirements, Olink recommends the following general safety guidelines in all
laboratory and manufacturing areas:
•
Use the appropriate personal protective equipment (PPE): safety glasses, fully enclosed shoes, lab coats, and gloves,
according to your laboratory safety practices.
•
Know the locations of all safety equipment (fire extinguishers, spill kits, eyewashes/ showers, first-aid kits, safety data
sheets, etc.), emergency exit locations, and emergency/injury reporting procedures.
•
Do not eat, drink, or smoke in lab areas.
•
Maintain clean work areas.
•
Wash hands before leaving the lab.
•
Notify Olink before servicing if biohazardous or radioactive materials are used on the instrument.
•
When handling biohazardous materials or using biohazardous material on the instrument, use appropriate personal
protective equipment. Adhere to Biosafety in Microbiological and Biomedical Laboratories (BMBL), a publication from
the Centers for Disease Control and Prevention, and your lab’s safety protocol to limit biohazard risks. If biohazardous
materials are used, label the equipment properly as a biohazard. For more information, see the BMBL guidelines online
at cdc.gov/biosafety/publications/index.htm.
10.2 Instrument Safety
Service the system by authorized personnel only.
WARNING: Do not modify this instrument or system. Unauthorized modifications may
create a safety hazard.
WARNING: PHYSICAL INJURY HAZARD. 2-person lift. Use proper lifting techniques.
The instrument weighs approximately 41.5 kg (91.5 lbs.). If you choose to lift or
move the instrument after it has been installed, do not attempt to do so without the
assistance of at least one more person. Use appropriate moving equipment and proper
lifting techniques to minimize the risk of physical injury.
CAUTION: HOT SURFACE HAZARD. The thermal cycler chuck gets hot and can burn
your skin. Use caution when working near the chuck.
CAUTION: Before you power down the instrument, be sure that you create a tab made
from a piece of tape and hang it out of the shuttle door. Otherwise, you will not be able
to open the shuttle later.
59
10.2.1 Symbols on the Instrument or in This Document
The following table describes the hazard symbols used on labels on the instrument (Figure 16) or in this document.
Symbol
Location on
Instrument
Description
Rear of instrument
Hazard. Consult the user guide for further information.
On the instrument
drawer
Hot surface hazard. Do not touch; potential for personal injury.
Rear of instrument
Electricity hazard. Indicates high electricity levels and a threat of
electric shock from machines or equipment in the vicinity. You may
suffer severe injuries or death.
On the instrument
drawer
Potential biohazard.
Rear of instrument
Lifting hazard.
Front of instrument
Power and standby symbol.
Rear of instrument
The Power switch is in the Off position.
Rear of instrument
The Power switch is in the On position.
Inside of instrument; Protective conductor terminal (main ground). It must be connected to
for service personnel earth ground before any other electrical connections are made to the
only.
instrument or system.
Rear of instrument
To minimize negative environmental impact from electronic waste
disposal, do not dispose of electronic waste in unsorted municipal
waste.
Follow local municipal waste ordinances for proper disposal provision.
Contact customer service for information about responsible disposal
options.
LAN
HDMI
POWERED BY
FLUIDIGM
USB
USB
COMPRESSION AIR INLET
(OPTIONAL)
70–90 PSI ONLY
AC 100–240V
50–60Hz, 8A
Rear of instrument
Figure 16. Safety symbols on the Signature Q100 instrument
10.3 Electrical Safety
60
NOTE: The main power disconnect is on the rear panel of the instrument.
WARNING: ELECTRICAL HAZARD. DO NOT REMOVE THE COVERS. Electrical shock can
result if the system is operated without its protective covers. No internal components
under the covers are serviceable by the user.
WARNING: ELECTRICAL HAZARD. Plug the system into a properly grounded
receptacle with adequate current capacity.
10.4 Chemical Safety
The responsible individuals must take the necessary precautions to ensure that the surrounding workplace is safe and that
system operators are not exposed to hazardous levels of toxic substances. When working with any chemicals, refer to the
manufacturer or supplier’s applicable safety data sheets (SDSs).
61
www.olink.com
Olink Proteomics, Dag Hammarskj&ouml;lds v&auml;g 52B, SE-752 37 Uppsala, Sweden
For technical support contact [email protected].
For Research Use Only. Not for use in diagnostic procedures.
All information in this publication is subject to change without notice. Trademarks: Olink and the Olink logo are trademarks or registered trademarks
of Olink Proteomics AB in the United States and/or other countries. All other trademarks are the sole property of their respective owners.
FLDM-00459 Rev 02 &copy; 2021 Olink Proteomics AB. All rights reserved. 11/2021
1172 v1.1, 2022-01-25
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