EASY-Spray Series Ion Source User Guide

EASY-Spray Series Ion Source User Guide
Thermo
EASY-Spray Series
Ion Source
User Guide
60053-97260 Revision C
July 2015
© 2015 Thermo Fisher Scientific Inc. All rights reserved.
Acclaim, Accucore, EASY-nLC, EASY-Spray, EASY-Spray NG, Endura MD, Foundation, nanoViper, Optima,
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Release history: Rev A, May 2012; Rev B, May 2013; Rev C, July 2015
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C
Contents
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x
Compatible Mass Spectrometers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xi
Product Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xi
Contents of the EASY-Spray Series Ion Source Kits . . . . . . . . . . . . . . . . . . . . . xii
Cautions and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiii
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xv
Thermo Scientific
Chapter 1
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Advantages of Nanoelectrospray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Functional Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Source Housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Controller PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Controller Power Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Chapter 2
Installing the EASY-Spray Series Ion Source . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
Installing the Ion Source onto the Mass Spectrometer . . . . . . . . . . . . . . . . . . . . . 8
Connecting the Controller Power Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Connecting the EASY-Spray P-Bus Temp Control Cable . . . . . . . . . . . . . . . . . 13
Configuring the Venting Tee Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Adjusting the Emitter Tip Position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Removing the EASY-Spray Series Source. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Chapter 3
Connecting the LCD Monitor and Video Camera. . . . . . . . . . . . . . . . . . . . . . . . . . .19
Connecting the Monitor and Camera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Adjusting the Video Picture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Chapter 4
Connecting the EASY-Spray Column Plumbing. . . . . . . . . . . . . . . . . . . . . . . . . . . .23
Tools and Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Installing the EASY-Spray Column for the First Time. . . . . . . . . . . . . . . . . . . . 24
Using the nanoViper Fitting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Connecting the LC to the EASY-Spray Column . . . . . . . . . . . . . . . . . . . . . . . . 27
Connecting an EASY-nLC 1000 Instrument to the EASY-Spray Column . . . 27
Connecting an EASY-nLC II Instrument to the EASY-Spray Column . . . . . 30
Connecting an RSLCnano System to the EASY-Spray Column. . . . . . . . . . . 33
EASY-Spray Series Ion Source User Guide
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Contents
Chapter 5
Configuring the Mass Spectrometer for NSI Mode . . . . . . . . . . . . . . . . . . . . . . . .37
Configuring the EASY-Spray NG Source’s NSI Parameters. . . . . . . . . . . . . . . . 37
Configuring the EASY-Spray Source’s NSI Parameters . . . . . . . . . . . . . . . . . . . 38
Selecting the Source for the LCQ Deca XP Max Mass Spectrometer . . . . . . . . . 40
Chapter 6
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
Guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Preparing the Work Area. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Tools and Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Cleaning the EASY-Spray Column Emitter Tip . . . . . . . . . . . . . . . . . . . . . . . . 43
Replacing the Controller PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Chapter 7
Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47
Chapter 8
Replaceable Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51
Spare Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Consumables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .53
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .55
vi
EASY-Spray Series Ion Source User Guide
Thermo Scientific
F
Figures
Figure 1.
Figure 2.
Figure 3.
Figure 4.
Figure 5.
Figure 6.
Figure 7.
Figure 8.
Figure 9.
Figure 10.
Figure 11.
Figure 12.
Figure 13.
Figure 14.
Figure 15.
Figure 16.
Figure 17.
Figure 18.
Figure 19.
Figure 20.
Figure 21.
Figure 22.
Figure 23.
Figure 24.
Figure 25.
Figure 26.
Figure 27.
Figure 28.
Figure 29.
Figure 30.
Figure 31.
Figure 32.
Figure 33.
Figure 34.
Figure 35.
Figure 36.
Thermo Scientific
nanoLC/MS system using the EASY-Spray ion source, camera, and monitor . . . . 1
EASY-Spray NG source housing (P/N ES082) . . . . . . . . . . . . . . . . . . . . . . . . . . 3
EASY-Spray source housing (P/N ES081) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Controller PCB located on the bottom of the source (P/N ES082) . . . . . . . . . . . 4
Controller power supply assembly (shown with a European input plug) . . . . . . . 5
Examples of the mass spectrometer ion sweep cones . . . . . . . . . . . . . . . . . . . . . . 9
EASY-Spray NG locking lever positions (top view) . . . . . . . . . . . . . . . . . . . . . . . 9
EASY-Spray locking lever positions (top view) . . . . . . . . . . . . . . . . . . . . . . . . . . 10
EASY-Spray NG source housing connection (ES082) . . . . . . . . . . . . . . . . . . . . 10
EASY-Spray source housing connection (ES081) . . . . . . . . . . . . . . . . . . . . . . . . 11
Power connection for the controller PCB (bottom view) . . . . . . . . . . . . . . . . . . 12
Screw to remove before installing the Tee holder . . . . . . . . . . . . . . . . . . . . . . . 13
Tee holder in the shipping configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Emitter positioning tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Gap positions in front of the mass spectrometer’s ion transfer tube (side view) . 15
X- and z-axes position controls on the source (P/N ES081) . . . . . . . . . . . . . . . 16
Y-axis position control (P/N ES082). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Camera and column installed on the EASY-Spray source (P/N ES081) . . . . . . . 20
EASY-Spray column . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
nanoViper fitting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Recommended plumbing locations on the venting tee (nanoViper example) . . 28
Tee holder secured to the bottom of the column holder . . . . . . . . . . . . . . . . . . 28
Example of a one-column setup with nanoViper fittings. . . . . . . . . . . . . . . . . . 29
Example of a two-column setup with nanoViper fittings and a Viper union . . . 30
LC line with sleeve, ferrule, and nut . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Column Out line with smaller fitting from the ZDV adapter union . . . . . . . . . 32
Example of a two-column setup for the EASY-nLC II . . . . . . . . . . . . . . . . . . . 33
Example of a one-column setup for an RSLCnano system (direct injection) . . . 34
Example of a two-column setup for an RSLCnano system (preconcentration) . 35
Ion Source page in the Tune window for the Orbitrap Fusion (example) . . . . . 38
Tune Plus window for an NSI-configured Velos Pro (example) . . . . . . . . . . . . . 39
NSI Source dialog box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Wash cap installed over the column emitter tip . . . . . . . . . . . . . . . . . . . . . . . . 43
EASY-Spray with the new controller PCB and extra cover plate (bottom view). 44
Controller PCB (P/N ES233) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Bottom of the EASY-Spray source without the controller PCB. . . . . . . . . . . . . 45
EASY-Spray Series Ion Source User Guide
vii
Figures
viii
EASY-Spray Series Ion Source User Guide
Thermo Scientific
P
Preface
The EASY-Spray Series Ion Source User Guide describes the hardware components, and
provides installation and configuration procedures for the Thermo Scientific™ EASY-Spray™
Series ion sources.
Contents
• Related Documentation
• Compatible Mass Spectrometers
• Product Requirements
• Contents of the EASY-Spray Series Ion Source Kits
• Cautions and Special Notices
• Contacting Us
 To suggest changes to the documentation
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Thermo Scientific
EASY-Spray Series Ion Source User Guide
ix
Preface
Related Documentation
In addition to this guide, you can also access the Preinstallation Requirements Guide, Getting
Connected Guide, Getting Started Guide, and Hardware Manual for your specific
Thermo Scientific mass spectrometer as PDF files from the data system computer.
 To view the product manuals
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x
EASY-Spray Series Ion Source User Guide
Thermo Scientific
Preface
Compatible Mass Spectrometers
Use the EASY-Spray Series ion source with the appropriate Thermo Scientific mass
spectrometers (MSs) for nanoelectrospray (commonly referred to as nanoES or nanospray)
analysis. Table 1 lists several compatible MSs.
Table 1. Ion sources and compatible mass spectrometers
Ion source model
Thermo Scientific mass spectrometer
EASY-Spray NG
• Endura MD™
• Orbitrap Fusion™ Series
• TSQ Endura™ and TSQ Quantiva™
EASY-Spray
•
•
•
•
•
Exactive™ and Q Exactive™
Orbitrap™ Series
LTQ™ Series
TSQ Quantum™ Ultra™
LCQ™ Deca XP Max
For information about your specific mass spectrometer, refer to the instrument manuals.
Product Requirements
Table 2 lists the environmental requirements for the EASY-Spray Series ion source. However,
the requirements for the mass spectrometer supersede these requirements. Refer to the
“Operating Environment” chapter in the preinstallation requirements guide for your
Thermo Scientific mass spectrometer.
CAUTION The EASY-Spray Series source must connect to a certified Thermo Scientific
mass spectrometer, which supplies high voltage capable of delivering a maximum of 8 kV
and 100 μA.
If you connect the EASY-Spray Series source to another type of mass spectrometer, you
might impair the protection provided by the equipment.
Table 2. EASY-Spray Series ion source environmental requirements
Thermo Scientific
Parameter
Specification
Altitude
Up to 2000 m (6562 ft)
Humidity
Maximum relative humidity of 80% for temperatures up to
31 °C (88 °F), decreasing linearly to 50% relative humidity at
40 °C (104 °F)
Operating environment
Pollution degree 2 (indoor environment; normally only
nonconductive pollution)
Temperature
Ambient temperature: 15–27 °C (59–81 °F)
EASY-Spray Series Ion Source User Guide
xi
Preface
Contents of the EASY-Spray Series Ion Source Kits
Table 3 lists the parts supplied in the EASY-Spray NG Ion Source Kit (P/N ES082) and the
EASY-Spray Ion Source Kit (P/N ES081), which are identical except for the source housing.
For a list of replaceable parts, see Chapter 8, “Replaceable Parts.”
Table 3. Contents of the EASY-Spray Series ion source kits (Sheet 1 of 2)
Image
Description
Quantity
Part number
Provided in the EASY-Spray NG Ion Source Kit (P/N ES082) only
1
—a
1
—b
EASY-Spray column, 15 cm × 3 μm ID
1
ES800
Controller power supply assembly:
1
ES231
Dino-Lite™ digital video camera and
power supply unit (not shown)
1
ES216
Monitor, power supply unit, and BNC
adapter (not shown)
1
ES217
EASY-Spray NG housing
Provided in the EASY-Spray Ion Source Kit (P/N ES081) only
EASY-Spray housing
Actual temperature
C
Set temperature
40
45
35
50
30
OFF
Heater
55
C
60
EASY Spray
Provided in both ion source kits
• Power supply unit, 24 Vdc output
(shown without the input plug)
• Power adapter cable (not shown)
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EASY-Spray Series Ion Source User Guide
Thermo Scientific
Preface
Table 3. Contents of the EASY-Spray Series ion source kits (Sheet 2 of 2)
Image
Description
Quantity
Tools:
1 set
Part number
ES232
• Emitter positioning tool
• 3 mm hex key
1.5 mm hex key
a
1
—
A/B mixing/venting tee, stainless steel,
with three attached ferrules and nuts
1 set
SC901
Tee holder (for the mixing/venting tee)
1
ES234
Sleeves, PEEK™, 1/16 in. OD
3
IDEX™,
F-233
Wash cap (for the EASY-Spray column)
1
ES235
To order just the EASY-Spray NG source housing, you must order the EASY-Spray NG Ion Source Kit
(P/N ES082).
bTo
order just the EASY-Spray source housing, you must order the EASY-Spray Ion Source Kit (P/N ES081).
Cautions and Special Notices
Make sure that you follow the cautions and special notices presented in this guide. Cautions
and special notices appear in boxes; those concerning safety or possible damage also have
corresponding caution symbols.
This guide uses the following types of cautions and special notices.
CAUTION Highlights hazards to humans, property, or the environment. Each CAUTION
notice is accompanied by an appropriate CAUTION symbol.
Thermo Scientific
EASY-Spray Series Ion Source User Guide
xiii
Preface
IMPORTANT Highlights information necessary to prevent damage to software, loss of
data, or invalid test results; or might contain information that is critical for optimal
performance of the system.
Note Highlights information of general interest.
Tip Highlights helpful information that can make a task easier.
The EASY-Spray Series Ion Source User Guide contains the following caution-specific symbols
(Table 4).
Table 4. Caution-specific symbols and their meanings
Symbol
Meaning
Chemical hazard: Wear gloves and other protective equipment, as
appropriate, when handling toxic, carcinogenic, mutagenic, corrosive,
or irritant chemicals. Use approved containers and proper procedures
to dispose of waste oil and when handling wetted parts of the
instrument.
Hot surface: Before touching, allow any heated components to cool.
Risk of electric shock: This instrument uses voltages that can cause
electric shock. Before servicing, shut down the instrument and
disconnect it from line power.
Risk of eye injury: Eye injury could occur from splattered chemicals or
airborne particles. Wear safety glasses when handling chemicals or
servicing the instrument.
Trip obstacle: Be aware of cords or other objects located on the floor.
xiv
EASY-Spray Series Ion Source User Guide
Thermo Scientific
Preface
Contacting Us
There are several ways to contact Thermo Fisher Scientific for the information you need. You
can use your smartphone to scan a QR code, which opens your email application or browser.
Contact us
Customer Service and Sales
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us.customer-support.analyze
@thermofisher.com
us.techsupport.analyze
@thermofisher.com
 To find global contact information or customize your request
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Note To provide feedback for this document:
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Thermo Scientific
EASY-Spray Series Ion Source User Guide
xv
Preface
xvi
EASY-Spray Series Ion Source User Guide
Thermo Scientific
1
Introduction
The EASY-Spray Series ion source, shown in Figure 1, maintains excellent spray stability to
ensure efficient evaporation and ionization of the liquid samples—the key to achieving the
highest sensitivity at nano-flow rates.
Key benefits of the EASY-Spray Series are as follows:
• Simple and robust design for nano liquid chromatography (nanoLC)/MS integration
• Better separation with zero dead volume (ZDV) connections
• Increased productivity with the temperature-controlled EASY-Spray column™
Contents
• Advantages of Nanoelectrospray
• Functional Description
Figure 1.
nanoLC/MS system using the EASY-Spray ion source, camera, and monitor
Thermo Scientific
EASY-nLC™ series instrument
Thermo Scientific
Thermo Scientific mass spectrometer
with the EASY-Spray ion source
EASY-Spray Series Ion Source User Guide
1
1
Introduction
Advantages of Nanoelectrospray
Advantages of Nanoelectrospray
The use of electrospray (ESI) has evolved as a leading technique for generating intact,
gas-phase ions from thermally labile, polar analytes in solution. In this technique, an emitter
(a capillary tube or needle) induces ionization at a controlled distance from a counter
electrode. Direct current (dc) voltage is applied, either to the needle or to the solvent, to
produce a strong electrical field at the emitter tip. The electric field excites the ions in the
solution as they leave the emitter tip. This interaction results in electrohydrodynamic
disintegration of the fluid, generation of droplets, and formation of an aerosol jet.
Conventional ESI employs flow rates from 1 μL/min to 1 mL/min. Expediting desolvation
and droplet shrinkage often requires a drying gas, thermal heating, or both, due to the high
volume of liquid that exits the emitter. Nanospray ionization (NSI), also known as
nanoelectrospray ionization (nanoESI or NSI), is a form of ESI that employs low rates of
10–1000 nL/min. NSI (or nanoESI) generally does not require a drying gas or thermal
heating. Compared with ESI, NSI tolerates a wider range of liquid compositions including
pure water.
As you lower the flow rate, a lower volume of mobile phase passes through the emitter,
producing smaller aerosol droplets. This makes NSI more effective than conventional ESI at
concentrating the analyte at the emitter tip, producing significant increases in sensitivity
demonstrated by the signal response of the mass spectrometer.
Note The mass spectrometer’s instrument control application uses the terms nanospray
and NSI.
Functional Description
This section describes the following principal components of the EASY-Spray Series source
and their functions:
• Source Housing
• Controller PCB
• Controller Power Supply
2
EASY-Spray Series Ion Source User Guide
Thermo Scientific
1 Introduction
Functional Description
Source Housing
The EASY-Spray Series source housing (Figure 2 or Figure 3) arrives assembled and is easy to
install on the appropriate mass spectrometer (see Table 1 on page xi). The source housing
includes two locking levers, two observation windows, a position-adjustable column holder,
and a controller printed circuit board (PCB). For installation instructions, see “Installing the
Ion Source onto the Mass Spectrometer” on page 8.
Figure 2.
EASY-Spray NG source housing (P/N ES082)
Opening for the camera
Source housing
Column holder
Figure 3.
EASY-Spray source housing (P/N ES081)
Opening for the camera
Source housing
Column holder
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Introduction
Functional Description
Using the top and front observation windows, you can view the tip of the emitter positioning
tool while you move it into position. To enhance your view of the tool and then later of the
emitter, connect the digital video camera and LCD monitor. For instructions, see Chapter 3,
“Connecting the LCD Monitor and Video Camera.”
The column holder is mounted onto the front observation window and includes a position
control knob. Use the control knob to align the z axis (front-to-back) of the emitter
positioning tool (Figure 14 on page 15) with the mass spectrometer’s ion transfer tube. To
align the tool’s x-axis (side-to-side) and y-axis position (up-and-down), use the controls that
are built into the EASY-Spray Series housing. For instructions, see “Adjusting the Emitter Tip
Position” on page 15.
Controller PCB
The EASY-Spray Series source contains a controller PCB that powers and controls the heat
generated for the connected EASY-Spray column. The controller PCB includes an input
power socket, an output heater socket, a temperature control dial, and an actual temperature
display. The bottom panel for the EASY-Spray NG source (Figure 4) is slightly different from
that of the EASY-Spray source.
Figure 4.
Controller PCB located on the bottom of the source (P/N ES082)
Actual temperature display
Controller PCB (not visible)
Input power connection
The controller power supply unit connects to the input power socket (labeled P-BUS), which
is located on the bottom right side of the EASY-Spray Series source. The heater cable from the
EASY-Spray column connects to the heater socket, which is located on the front and labeled
“Heater.”
The temperature control dial has an OFF setting and temperature settings in 5 °C increments
from 30 through 60 °C. As the column heats up, the actual temperature appears on the
display.
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1 Introduction
Functional Description
Tip If you have a Thermo Scientific EASY-nLC 1000 or EASY-nLC II instrument and the
optional EASY-Spray P-Bus Temp Control Cablea (P/N 70005-63055), you can connect
this cable between the LC instrument and the EASY-Spray Series source to power the
source and enable touch-screen control of the EASY-Spray column’s temperature.
For cable installation instructions, see “Connecting the EASY-Spray P-Bus Temp Control
Cable” on page 13. For information about controlling the column temperature through
the LC instrument’s touch screen or the Xcalibur Instrument Setup window, refer to the
EASY-nLC Series instrument manuals.
a
For use with the touch-screen application version 3.2 and later
Controller Power Supply
Figure 5 shows the controller power supply assembly that consists of a wall-mounted power
supply unit with a detachable input plug and a power adapter cable. The other end of the
power adapter cable connects to the input power socket (labeled P-BUS) located on the
bottom of the EASY-Spray Series source to provide the 24 Vdc input for the controller PCB.
Figure 5.
Controller power supply assembly (shown with a European input plug)
Power supply unit with output cord
Power adapter cable
The total length of the output cord and power adapter cable is approximately 274 cm (9 ft).
Make sure that an electrical wall outlet is within reach for the power supply unit and that you
provide suitable clearance around it for cooling.
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Introduction
Functional Description
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Installing the EASY-Spray Series Ion Source
Follow these procedures to install or remove the EASY-Spray Series ion source. The only tool
required is the 3 mm hex key.
Note The complete EASY-Spray Series source installation, including the digital camera
and LCD monitor, requires three electrical wall outlets. Ensure that your lab has the
appropriate outlets near the nanoLC/MS system.
Contents
• Installing the Ion Source onto the Mass Spectrometer
• Connecting the Controller Power Supply
• Configuring the Venting Tee Holder
• Adjusting the Emitter Tip Position
• Removing the EASY-Spray Series Source
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Installing the EASY-Spray Series Ion Source
Installing the Ion Source onto the Mass Spectrometer
Installing the Ion Source onto the Mass Spectrometer
Before you install the EASY-Spray Series source, follow the procedure to prepare the mass
spectrometer.
 To prepare the mass spectrometer
1. If any other source is installed, remove it from the mass spectrometer after it has cooled to
room temperature.
For instructions, refer to the mass spectrometer’s documentation.
CAUTION Hot surface. Accidental contact with the part of the API probe or spray
insert located inside the housing can severely burn you, as this area of the part can
reach 350 °C (662 °F). In addition, although the exterior surface of the housing and
the exposed part of the probe or spray insert fall below the limit of 70 °C (158 °F) for
accessible metal surfaces, they too pose a burn hazard. Therefore, handle ALL heated
parts with extreme caution.
• Before removing the API probe, spray insert, or housing, allow the part to cool to
room temperature (approximately 20 minutes) before you touch it.
• If the mass spectrometer connects to an LC system, leave the solvent flow from
the LC pump on while the probe or spray insert cools to room temperature.
2. If the ion sweep cone (Figure 6) is installed, remove it from the mass spectrometer by
grasping the outer ridges of the ion sweep cone and pulling it off.
The spray cone is directly behind the ion sweep cone (Figure 9 on page 10 or Figure 10
on page 11).
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2 Installing the EASY-Spray Series Ion Source
Installing the Ion Source onto the Mass Spectrometer
Figure 6.
Examples of the mass spectrometer ion sweep cones
Ion sweep cone for the Orbitrap Fusion
mass spectrometer
Ion sweep cone for the legacy mass
spectrometers (shown is the offset orifice
type for ESI, H-ESI, and APCI)
 To install the source onto the mass spectrometer
1. Follow the procedure “To prepare the mass spectrometer” on page 8.
2. Depending on your source type, unlock the source’s locking levers as follows:
• For the EASY-Spray NG source, rotate the locking levers until they are horizontal
(Figure 7).
Figure 7.
EASY-Spray NG locking lever positions (top view)
Unlocked (horizontal) position
Locked
(vertical)
position
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Installing the EASY-Spray Series Ion Source
Installing the Ion Source onto the Mass Spectrometer
–or–
• For the EASY-Spray source, rotate the locking levers toward the front of the source
(right lever in Figure 8).
Figure 8.
EASY-Spray locking lever positions (top view)
Locked position
Unlocked position
Top observation window
3. Do one of the following:
• For the EASY-Spray NG source, align the three guide pins on the back of the source
with the guide pin holes on the front of the mass spectrometer (Figure 9).
Figure 9.
EASY-Spray NG source housing connection (ES082)
MS spray cone
High voltage (HV)
connector
Guide pins on the MS (for example, Orbitrap
Fusion) ion source mount assembly
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EASY-Spray Series Ion Source User Guide
Spring-loaded bolt
delivers HV to the
column
Guide holes on the back of the
EASY-Spray NG source housing
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2 Installing the EASY-Spray Series Ion Source
Installing the Ion Source onto the Mass Spectrometer
–or–
• For the EASY-Spray source, align the two guide pin holes on the back of the source
with the guide pins on the front of the mass spectrometer (Figure 10).
Note Your legacy mass spectrometer might have a slightly different mount
assembly, ion sweep cone, or both.
Figure 10. EASY-Spray source housing connection (ES081)
MS spray cone
Guide pins on the legacy MS ion
source mount assembly
HV connector
Spring-loaded
bolt delivers HV
to the column
Guide pin holes on the back of the
EASY-Spray source housing
4. Carefully press the source onto the mass spectrometer.
5. Lock the source’s locking levers.
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Installing the EASY-Spray Series Ion Source
Connecting the Controller Power Supply
Connecting the Controller Power Supply
If the LC instrument includes the EASY-Spray P-Bus Temp Control Cable
(P/N 70005-63055), you can skip this section and go to Connecting the EASY-Spray P-Bus
Temp Control Cable.
CAUTION To avoid an electric shock, always use the detachable input plug that is
appropriate for your country or region and comes with the power supply unit.
 To connect the controller power supply unit
1. Turn the temperature dial on the EASY-Spray Series source to the OFF position.
2. If not already connected, connect the detachable input plug and power adapter cable to
the power supply unit (Figure 5 on page 5).
3. Plug the power supply unit into an electrical wall outlet.
4. Connect the power adapter cable to the P-BUS socket located on the bottom, right side
of the source (Figure 17 on page 17) as follows:
a. Align the red dot on the plug with the red dot on the socket, which is on the right
side of the socket (Figure 11).
Figure 11. Power connection for the controller PCB (bottom view)
Red dot on the socket
Red dot on the plug
b. Push the plug upward into the socket.
CAUTION After completing the power supply connections, route the output cord so that
it is not a trip hazard.
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2 Installing the EASY-Spray Series Ion Source
Connecting the EASY-Spray P-Bus Temp Control Cable
Connecting the EASY-Spray P-Bus Temp Control Cable
If you have the optional EASY-Spray P-Bus Temp Control Cable (P/N 70005-63055) and an
EASY-nLC 1000 or EASY-nLC II instrument, use this cable to control the EASY-Spray
column temperature and provide power to the EASY-Spray Series source. This cable replaces
the use of the controller power supply unit.
 To connect the EASY-Spray P-Bus Temp Control Cable
1. Connect one end of the cable to the P-BUS socket on the back of the LC instrument.
2. Connect the other end of the cable to the P-BUS socket on the bottom of the source.
Configuring the Venting Tee Holder
Tip The EASY-Spray NG ion source (ES082) ships with the Tee holder attached to the
column holder. If you have the EASY-Spray ion source (ES081), you can order the
optional Tee holder to support the venting tee; see Chapter 8, “Replaceable Parts.”
Use this procedure to configure the (venting) Tee holder before inserting the emitter
positioning tool or the EASY-Spray column.
Follow these procedures, as applicable:
• To install the optional Tee holder under the column holder
• To configure the Tee holder
 To install the optional Tee holder under the column holder
1. Remove the screw located on the bottom of the column holder (Figure 12).
Figure 12. Screw to remove before installing the Tee holder
Remove this screw.
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Installing the EASY-Spray Series Ion Source
Configuring the Venting Tee Holder
2. Hold the Tee holder (Figure 13) under the column holder with one hand. With the other
hand, insert and tighten the hex socket-head bolt through the bottom of the Tee holder
and the column holder.
The bolt is provided with the Tee holder.
Figure 13. Tee holder in the shipping configuration
This end goes below
the column holder.
Sliding lock
Hex socket-head bolt
 To configure the Tee holder
1. Bend the top part of the Tee holder down to the extended position (Figure 22 on
page 28).
2. Move the sliding lock toward the column holder.
Note If the ion source is not to be used for an extended period of time, unlock and
position the Tee holder as shown in Figure 13.
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Installing the EASY-Spray Series Ion Source
Adjusting the Emitter Tip Position
Adjusting the Emitter Tip Position
Before installing the EASY-Spray column, use the emitter positioning tool (Figure 14) and
3 mm hex key to adjust the position of the column holder.
Figure 15 shows the emitter positioning tool touching the mass spectrometer’s ion transfer
tube. The length of the tool’s tip ensures the 3 mm gap for the EASY-Spray column’s emitter.
Figure 14. Emitter positioning tool
Figure 15. Gap positions in front of the mass spectrometer’s ion transfer tube (side view)
0 mm gap between the ion transfer tube
and the emitter positioning tool
Emitter positioning tool
Mass spectrometer
spray cone
3 mm gap between the ion transfer tube
and an actual emitter
EASY-Spray column
(shown without its cable)
Ion transfer tube
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Installing the EASY-Spray Series Ion Source
Adjusting the Emitter Tip Position
 To adjust the emitter tip position
Tip To enhance your view of the emitter positioning tool’s tip, install the video
camera and monitor before you begin this procedure. For instructions, see Chapter 3,
“Connecting the LCD Monitor and Video Camera.”
1. Retract the z-axis (front-to-back) position by turning the control knob located on the
column holder counterclockwise (Figure 16).
2. Insert the emitter positioning tool into the top of the column holder, and then slowly
slide it forward until it clicks into place.
CAUTION High voltage. To prevent electric shock, never insert a foreign object into
the source opening, which is intended for the emitter positioning tool and the
EASY-Spray column only.
3. Align the x-axis (side-to-side) position of the emitter positioning tool to the bore of the
ion transfer tube by inserting the hex key into the left-side hex socket-head screw
(Figure 16).
Figure 16. X- and z-axes position controls on the source (P/N ES081)
Inserting the emitter
positioning tool
Z-axis position control (knob)
X-axis position control
(hex socket-head screw)
Y
Z
X
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Installing the EASY-Spray Series Ion Source
Removing the EASY-Spray Series Source
4. Align the y-axis (up-and-down) position of the emitter positioning tool to the bore of the
ion transfer tube by inserting the hex key into the bottom hex socket-head screw
(Figure 17).
Figure 17. Y-axis position control (P/N ES082)
Y-axis position control
One of the hex socket-head screws
for the controller PCB (P/N ES233)
5. Adjust the z-axis (front-to-back) position of the emitter positioning tool’s tip by turning
the control knob located on the front of the column holder (Figure 16 on page 16). Move
the tool’s tip so that it gently touches the bore of the ion transfer tube.
IMPORTANT The fused-silica emitter is fragile. Make sure that the emitter
positioning tool gently touches the ion transfer tube when you adjust its position or
you will damage the actual emitter when fully inserted, requiring you to replace the
EASY-Spray column.
6. After you adjust the tool tip’s position, slowly remove the emitter positioning tool.
The position of the column holder is now calibrated for use with any EASY-Spray column.
You must repeat this procedure if you later change the x-, y-, or z-axis position.
Removing the EASY-Spray Series Source
Follow this procedure if you must remove the EASY-Spray Series ion source from the mass
spectrometer.
 To remove the source from the mass spectrometer
1. In the applicable instrument control application, place the mass spectrometer in Standby
mode.
For instructions, refer to the mass spectrometer’s documentation.
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Installing the EASY-Spray Series Ion Source
Removing the EASY-Spray Series Source
2. Turn the source’s temperature dial to the OFF position.
CAUTION Hot surface. The maximum safety limit for heated surfaces is 70 °C
(158 °F). Although the surface temperature of the EASY-Spray Series ion source falls
below this maximum, it can still severely burn you, reaching approximately 50 °C
(122 °F) with the heater unit turned on. Allow the source to cool to room
temperature (approximately 20 minutes) before you touch it.
3. Turn off the LC instrument.
4. Disconnect the LC plumbing from the EASY-Spray column, and then remove the
EASY-Spray column.
Tip Do not pull on the cable from the EASY-Spray column. Use the plug to
disconnect the column from the heater socket.
5. If the Tee holder is installed, move the sliding lock away from the column holder, and
then bend the unattached end upward (Figure 13 on page 14).
6. Remove the camera as follows:
a. Unplug the camera power supply unit from the electrical wall outlet.
b. Disconnect the camera’s yellow video connector from the back of the monitor and
the black power connector from the camera power supply unit.
c. Using the 1.5 mm hex key, loosen the hex socket-head screw securing the camera,
and then remove the camera.
7. Do one of the following, as applicable:
• Disconnect the EASY-Spray P-Bus Temp Control Cable from the source.
• Unplug the controller power supply unit from the electrical wall outlet, and then
disconnect the output cable from the source.
8. Unlock the source’s locking levers (Figure 7 on page 9 and Figure 8 on page 10).
9. Grasp the source housing with both hands and slowly pull it away from the mass
spectrometer.
After you remove the EASY-Spray Series ion source from the mass spectrometer, place it in its
original shipping box. The EASY-Spray Series source does not require cleaning.
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Connecting the LCD Monitor and Video Camera
Follow these procedures to connect the LCD monitor and digital video camera, and to adjust
the video picture. The only tool required is the 1.5 mm hex key.
Contents
• Connecting the Monitor and Camera
• Adjusting the Video Picture
Connecting the Monitor and Camera
To enhance your view of the emitter tip, install the provided LCD monitor and digital video
camera (see Table 3 on page xii).
 To connect the monitor and camera
1. Follow the procedure “To install the source onto the mass spectrometer” on page 9.
2. Plug the two power supply units into electrical wall outlets.
3. Install the camera as follows:
a. Insert the camera into the opening above the top observation window with the
focusing wheel facing toward you (Figure 18).
b. Using the 1.5 mm hex key, tighten the hex socket-head screw on the left side of the
top observation window (Figure 16 on page 16) until it touches the camera—do not
overtighten the screw.
c. Connect the camera’s black power connector to its power supply unit.
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Connecting the LCD Monitor and Video Camera
Connecting the Monitor and Camera
Figure 18. Camera and column installed on the EASY-Spray source (P/N ES081)
Camera light button
Camera focusing wheel
Hex socket-head screw to
secure the camera
EASY-Spray column
4. On the back of the monitor, make the following connections:
• BNC adapter to one of the Video IN ports
• Camera’s yellow video connector to the BNC adapter
• Output cord from the other power supply unit to the DC 12V IN socket
CAUTION After completing the power supply connections, route the output cords so
that they are not a trip hazard.
5. Place the monitor on top of the mass spectrometer near the source by using its
support arm.
Make sure that the monitor is not too close to the edge of the instrument.
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Connecting the LCD Monitor and Video Camera
Adjusting the Video Picture
Adjusting the Video Picture
Use this procedure to focus the picture from the video camera. For additional information,
refer to the manuals for the monitor and camera.
 To adjust the video picture
1. Follow the procedure “To connect the monitor and camera” on page 19.
2. Turn on the monitor by pressing its POWER button.
The picture appears after a few seconds and is probably blurred. If there is no picture,
press the monitor’s SOURCE button to change to the other video input channel.
3. If the picture is too dark, press the light button at the top of the camera (Figure 18 on
page 20).
4. Adjust the picture by using the camera’s focusing wheel (Figure 18 on page 20).
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Connecting the LCD Monitor and Video Camera
Adjusting the Video Picture
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Connecting the EASY-Spray Column Plumbing
Follow these procedures to connect the plumbing from the Thermo Scientific LC instrument
to the EASY-Spray column.
Contents
• Tools and Supplies
• Installing the EASY-Spray Column for the First Time
• Using the nanoViper Fitting
• Connecting the LC to the EASY-Spray Column
Tools and Supplies
Table 5 lists the required tools and supplies for configuring the LC plumbing and EASY-Spray
column connections. Not all parts are required.
Note The kit for the EASY-Spray Series ion source supplies the venting tee, fittings, and
sleeves only. You are responsible for supplying the remaining parts and tools.
Table 5. Tools and supplies (Sheet 1 of 2)
Image
Description
Part number
One- and two-column setups
Thermo Scientific
A/B mixing/venting tee, stainless
steel, with three attached ferrules
and nuts
SC901
Sleeves, PEEK, 1/16 in. OD
IDEX, F-233
Wrench, open end, 1/4 in.
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Connecting the EASY-Spray Column Plumbing
Installing the EASY-Spray Column for the First Time
Table 5. Tools and supplies (Sheet 2 of 2)
Image
Description
Part number
—
Gloves, lint-free and powder-free
Fisher Scientific™ 19-120-2947a
UnitySM Lab Services:
• 23827-0008 (size medium)
• 23827-0009 (size large)
Two-column setup
Any nanoViper™-equipped
precolumn
This image shows the Acclaim™
PepMap100 Nano-Trap.
—
a
(For the PepMap100
Nano-Trap) Fisher Scientific,
164705
Viper™ union
(for use with the EASY-nLC 1000)
Fisher Scientific 6040.2304
ZDV adapter union (1/16 to
1/32 in.) and plug (not shown)
(for use with the EASY-nLC II)
IDEX, P-881
UltiMate™ 3000 RSLCnano
EASY-Spray Application Kit
Thermo Scientific Dionex™,
6720.0395
Multiple sizes are available.
Installing the EASY-Spray Column for the First Time
The EASY-Spray column (Figure 19) contains an integrated heatable column and a
fused-silica emitter. For available columns, see Chapter 8, “Replaceable Parts.”
IMPORTANT The EASY-Spray column assembly includes an emitter protective cover. If
you push back this cover by hand, you could damage the fused-silica emitter and make the
EASY-Spray column unusable.
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Connecting the EASY-Spray Column Plumbing
Installing the EASY-Spray Column for the First Time
Figure 19. EASY-Spray column
nanoViper fitting for the
LC connection
Emitter protective cover
Protective cap
Heater cable and plug
 To install the EASY-Spray column
1. If the Tee holder is attached to the column holder, follow the procedure “To configure the
Tee holder” on page 14.
2. Follow the procedure “To adjust the emitter tip position” on page 16.
Note You can skip this step after the first time if you have not adjusted the column
holder’s position.
3. Remove the protective cap on the end of the EASY-Spray column (Figure 19).
4. With the cable on the bottom, slowly push the tip of the EASY-Spray column into the
column holder opening until it clicks into place.
As the EASY-Spray column moves forward into the source, the protective cover over the
emitter retracts to expose the emitter tip.
5. Connect the cable to the front output heater socket.
Note Do not pull on the cable from the EASY-Spray column. Use the plug to
connect and disconnect the column.
6. Follow the appropriate procedure in “Connecting the LC to the EASY-Spray Column” on
page 27.
7. Do one of the following to set the temperature for the EASY-Spray column:
• Follow the procedure “Connecting the EASY-Spray P-Bus Temp Control Cable” on
page 13.
You can set the column temperature by using either the EASY-nLC 1000 or the
EASY-nLC II instrument touch screen or the Thermo Xcalibur Instrument Setup
window. For instructions, refer to the LC instrument manuals.
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Connecting the EASY-Spray Column Plumbing
Using the nanoViper Fitting
• Follow the procedure “Connecting the Controller Power Supply” on page 12, and use
the temperature control dial on the front of the source.
Table 6 lists the display readings that can appear on the actual temperature display.
Table 6. Readings for the actual temperature display
a
Display
Description
--
EASY-Spray column is not connected.
xx.
Actual temperature while heating up
xx
Actual temperature, stabilized
BL
Disconnect and reconnect power to the source.a
If the display does not change, you must replace the controller PCB. See “Replacing the Controller
PCB” on page 44.
Using the nanoViper Fitting
For some of the LC instruments, the plumbing connects to the EASY-Spray column through
several nanoViper fittings, such as the one shown in Figure 20. Although these fittings
can withstand ultra-high-performance LC (UHPLC) backpressures up to ~1034 bar
(~15 000 psi), they are fingertight fittings, which require only very small torques to seal.
Therefore, you must follow the procedure below to avoid damage by overtightening.
Figure 20. nanoViper fitting
PEEK sealing surface
Removable knurled tightening tool
Screw
 To use a nanoViper fitting
1. Insert the nanoViper fitting into the target port and slowly rotate the screw clockwise
until you feel resistance.
2. Using the black knurled fitting tool, tighten the screw clockwise to an angle of
0–45 degrees (1/8-turn).
3. Start operating the system at the desired working pressure and check the backpressure.
4. If the backpressure is too low, check the system for leaks.
For instructions, refer to the documentation for the LC instrument.
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Connecting the EASY-Spray Column Plumbing
Connecting the LC to the EASY-Spray Column
5. If the backpressure continues to be too low, return the system to atmospheric pressure.
IMPORTANT To extend the lifetime of the nanoViper fittings, open and close the
connections at only atmospheric system pressures. Opening and closing connections
at high system pressures can reduce the lifetime of the fittings.
6. Tighten the screw by as much as an additional 45 degrees. Do not turn the screw beyond
an angle of 90 degrees from where you felt the initial resistance.
IMPORTANT To prevent damage to the sealing surface of the nanoViper fitting, do
not overtighten the fitting.
Connecting the LC to the EASY-Spray Column
This section describes how to connect the one or two plumbing lines from a nanoLC
instrument to the EASY-Spray column in either a one- or two-column configuration. Table 5
on page 23 lists the required parts.
Note The EASY-nLC Series instruments identify the two solvent lines as “Column Out”
and “Waste In.”
CAUTION Wear protective gloves and eye wear when handling the solvent lines.
Follow the appropriate procedure:
• Connecting an EASY-nLC 1000 Instrument to the EASY-Spray Column
• Connecting an EASY-nLC II Instrument to the EASY-Spray Column, on page 30
• Connecting an RSLCnano System to the EASY-Spray Column, on page 33
Connecting an EASY-nLC 1000 Instrument to the EASY-Spray Column
Follow the appropriate procedure for a one- or two-column setup. The two-column setup
includes a precolumn connected in the column-out flow path to the venting tee.
Note Remove the nuts and ferrules from the venting tee.
• To plumb a one-column setup from an EASY-nLC 1000 instrument
• To plumb a two-column setup from an EASY-nLC 1000 instrument, on page 29
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Connecting the EASY-Spray Column Plumbing
Connecting the LC to the EASY-Spray Column
 To plumb a one-column setup from an EASY-nLC 1000 instrument
1. On the Column Out line, insert the nanoViper fitting into the venting tee’s side port as
shown in Figure 21, tighten the fitting by hand until you feel resistance, and then tighten
the fitting again by an additional 1/8- to 1/4-turn. Do not tighten the fitting by more
than a 1/4-turn.
Figure 21. Recommended plumbing locations on the venting tee (nanoViper example)
Location for the Column Out line
Location for the EASY-Spray column
Location for the Waste In line
CAUTION Because the fittings seal against each other inside the union, do not tighten
them by more than 1/4-turn (90 degrees). Overtightening the fittings can irreparably
damage their sealing surfaces.
For additional information about nanoViper fittings, see “Using the nanoViper
Fitting” on page 26.
2. On the Waste In line, insert the fitting into the venting tee’s bottom port, tighten the
fitting by hand until you feel resistance, and then tighten the fitting by an additional
1/8- to 1/4-turn. Do not tighten the fitting by more than a 1/4-turn.
3. Insert the hex key into the bolt that secures the (venting) Tee holder to the column
holder, loosen the hex socket-head bolt, and then slowly move the Tee holder forward
(Figure 22).
Figure 22. Tee holder secured to the bottom of the column holder
Tee holder (extended position)
Hex socket-head bolt
for the Tee holder
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4
Connecting the EASY-Spray Column Plumbing
Connecting the LC to the EASY-Spray Column
4. Connect the venting tee to the EASY-Spray column to complete the one-column setup
(Figure 23).
Figure 23. Example of a one-column setup with nanoViper fittings
Column Out line
EASY-Spray column
Waste In line
5. Adjust the Tee holder’s position so that it supports the nanoViper fitting, and then use the
hex key to tighten the hex socket-head bolt on the bottom of the column holder.
 To plumb a two-column setup from an EASY-nLC 1000 instrument
1. Assemble and connect the Column Out line as follows:
a. Insert the nanoViper fitting on the Column Out line into the Viper union, tighten
the fitting by hand until you feel resistance, and then tighten the fitting again by an
additional 1/8- to 1/4-turn (45–90 degrees). Do not tighten the fitting by more than
a 1/4-turn.
Note See the Caution on page 28.
b. Connect the other end of the union to the appropriate end of the nanoViper
precolumn.
Note Follow the recommended flow direction stated on the precolumn label.
c. Insert the fitting on the other end of the precolumn into the venting tee’s side port
(Figure 21 on page 28), and then tighten as noted in step 1a.
2. On the Waste In line, insert the fitting into the venting tee’s bottom port, tighten the
fitting by hand until you feel resistance, and then tighten the fitting again by an
additional 1/8- to 1/4-turn. Do not tighten the fitting by more than a 1/4-turn.
3. Insert the hex key into the bolt that secures the (venting) Tee holder to the column
holder, loosen the hex socket-head bolt, and then slowly move the Tee holder forward
(Figure 22 on page 28).
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Connecting the EASY-Spray Column Plumbing
Connecting the LC to the EASY-Spray Column
4. Connect the venting tee to the EASY-Spray column to complete the two-column setup
(Figure 24).
Figure 24. Example of a two-column setup with nanoViper fittings and a Viper union
Column Out line
EASY-Spray column
Waste In line
5. Adjust the Tee holder’s position so that it supports the nanoViper fitting, and then use the
hex key to tighten the hex socket-head bolt on the bottom of the column holder.
Tip When you install the two-column assembly, clear the One Column Setup check box
in the Maintenance menu (choose Maintenance > Devices, select the instrument, and
then select the Properties tab). For instructions, refer to “Setting Up the Properties for the
HPLC Device” in Chapter 4 of the EASY-nLC Series Getting Started Guide.
When the One Column Setup check box is clear (not selected), valve W on the
EASY-nLC 1000 moves to position 1–6 during the sample loading step. Excess solvent A
exits the venting tee and passes through valve W to the waste beaker.
Connecting an EASY-nLC II Instrument to the EASY-Spray Column
Follow the appropriate procedure for a one- or two-column setup. The two-column setup
includes a precolumn connected in the column-out flow path to the venting tee.
Note Remove the nuts and ferrules from the venting tee.
• To plumb a one-column setup from an EASY-nLC II instrument
• To plumb a two-column setup from an EASY-nLC II instrument
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4
Connecting the EASY-Spray Column Plumbing
Connecting the LC to the EASY-Spray Column
 To plumb a one-column setup from an EASY-nLC II instrument
1. Attach the nuts and ferrules to the Column Out and Waste In lines as follows:
a. Insert one of the lines into a sleeve, and then slowly push it forward until the line is
parallel with the end of the sleeve.
b. Slide the nut and then the ferrule onto the sleeved line until the sleeve extends past
the ferrule (Figure 25).
c. Repeat step 1a and step 1b with the second line.
Figure 25. LC line with sleeve, ferrule, and nut
Nut
Ferrule
Sleeve
2. On the Column Out line, insert the fittings into the venting tee’s side port (Figure 21 on
page 28), tighten the nut by hand until you feel resistance, and then use the 1/4 in.
wrench to tighten the nut until firmly secured—do not overtighten.
3. On the Waste In line, insert the fittings into the venting tee’s bottom port, tighten the nut
by hand until you feel resistance, and then use the 1/4 in. wrench to tighten the nut until
firmly secured—do not overtighten.
4. Insert the hex key into the bolt that secures the (venting) Tee holder to the column
holder, loosen the hex socket-head bolt, and then slowly move the Tee holder forward
(Figure 22 on page 28).
5. Connect the venting tee to the EASY-Spray column to complete the one-column setup.
6. Adjust the Tee holder’s position so that it supports the nanoViper fitting, and then use the
hex key to tighten the hex socket-head bolt on the bottom of the column holder.
Figure 23 on page 29 shows an example of a one-column setup for the EASY-nLC 1000
instrument, which uses nanoViper fittings.
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4
Connecting the EASY-Spray Column Plumbing
Connecting the LC to the EASY-Spray Column
 To plumb a two-column setup from an EASY-nLC II instrument
1. Assemble and connect the Column Out line as follows:
a. Remove the smaller fitting from the ZDV adapter union, insert the Column Out line
into the fitting, and then slowly push the line forward until it extends past the fitting
(Figure 26).
Figure 26. Column Out line with smaller fitting from the ZDV adapter union
Smaller fitting (IDEX P/N F-126S)
for the adapter union
b. With the plug inserted into the ZDV adapter union’s larger end, insert the
Column Out line into the union’s smaller end, and then tighten the smaller fitting
until you feel resistance.
c. Remove the plug from the adapter union, and then connect the adapter union’s larger
end to the appropriate end of the nanoViper precolumn.
Note Follow the recommended flow direction stated on the precolumn label.
d. Insert the fitting at the other end of the precolumn into the venting tee’s side port
(Figure 21 on page 28), and then tighten the fitting by an additional 1/8- to 1/4-turn
(45–90 degrees). Do not tighten the fitting by more than a 1/4-turn.
CAUTION Because the fittings seal against each other inside the union, do not
tighten them by more than 1/4-turn (90 degrees). Overtightening the fittings can
irreparably damage their sealing surfaces.
For additional information about nanoViper fittings, see “Using the nanoViper
Fitting” on page 26.
2. Assemble and connect the Waste In line as follows:
a. Attach a nut and ferrule to the Waste In line. See step 1a and step 1b in “To plumb a
one-column setup from an EASY-nLC II instrument” on page 31.
b. Insert the nut and ferrule fittings into the venting tee’s bottom port, tighten the nut
by hand until you feel resistance, and then use the 1/4 in. wrench to tighten the nut
until firmly secured—do not overtighten.
3. Insert the hex key into the bolt that secures the (venting) Tee holder to the column
holder, loosen the hex socket-head bolt, and then slowly move the Tee holder forward
(Figure 22 on page 28).
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4
Connecting the EASY-Spray Column Plumbing
Connecting the LC to the EASY-Spray Column
4. Connect the venting tee to the EASY-Spray column to complete the two-column setup
(Figure 27).
Figure 27. Example of a two-column setup for the EASY-nLC II
Column Out line
EASY-Spray column
Waste In line
5. Adjust the Tee holder’s position so that it supports the nanoViper fitting, and then use the
hex key to tighten the hex socket-head bolt on the bottom of the column holder.
Connecting an RSLCnano System to the EASY-Spray Column
Follow the appropriate procedure for a one- or two-column setup for an RSLCnano system.
The two-column setup includes a precolumn connected across the autosampler valve.
Note To connect the RSLCnano system to the EASY-Spray column, you must use the
Thermo Scientific Dionex UltiMate 3000 RSLCnano EASY-Spray Application Kit
(P/N 6720.0395).
• To plumb a one-column setup from an RSLCnano instrument
• To plumb a two-column setup from an RSLCnano instrument, on page 35
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Connecting the EASY-Spray Column Plumbing
Connecting the LC to the EASY-Spray Column
 To plumb a one-column setup from an RSLCnano instrument
1. Insert the nanoViper fitting on the 20 μm ID nanoViper capillary into port 5 of the
autosampler valve (Figure 28).
2. Insert the fitting on the other end of the capillary into the Viper union, tighten the fitting
by hand until you feel resistance, and then tighten the fitting again by an additional
1/8- to 1/4-turn (45–90 degrees). Do not tighten the fitting by more than a 1/4-turn.
CAUTION Because the fittings seal against each other inside the union, do not tighten
them by more than 1/4-turn (90 degrees). Overtightening the fittings can irreparably
damage their sealing surfaces.
For additional information about nanoViper fittings, see “Using the nanoViper
Fitting” on page 26.
3. Connect the union to the EASY-Spray column to complete the one-column setup
(Figure 28).
Figure 28. Example of a one-column setup for an RSLCnano system (direct injection)
EASY-Spray
column
20 μm ID × 65 cm
Viper union
20 μm ID
Autosampler
1 μL loop
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4
Connecting the EASY-Spray Column Plumbing
Connecting the LC to the EASY-Spray Column
 To plumb a two-column setup from an RSLCnano instrument
1. Connect the precolumn to ports 2 and 5 on one of the switching valves (Figure 29).
2. Insert the nanoViper fitting on the 20 μm ID nanoViper capillary into port 4 on the
same switching valve.
3. Insert the fitting on the other end of the capillary into the Viper union, tighten the fitting
by hand until you feel resistance, and then tighten the fitting again by an additional
1/8- to 1/4-turn (45–90 degrees). Do not tighten the fitting by more than a 1/4-turn.
Note See the Caution on page 34.
4. Connect the union to the EASY-Spray column to complete the two-column setup
(Figure 29).
Figure 29. Example of a two-column setup for an RSLCnano system (preconcentration)
EASY-Spray
column
Viper union
20 μm ID
20 μm ID
Column compartment
with two switching
valves
Precolumn
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4
Connecting the EASY-Spray Column Plumbing
Connecting the LC to the EASY-Spray Column
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5
Configuring the Mass Spectrometer for NSI Mode
Follow the applicable procedures to configure the Thermo Scientific mass spectrometer for
nanoelectrospray ionization (nanoESI or NSI) mode.
Contents
• Configuring the EASY-Spray NG Source’s NSI Parameters
• Configuring the EASY-Spray Source’s NSI Parameters
• Selecting the Source for the LCQ Deca XP Max Mass Spectrometer
Configuring the EASY-Spray NG Source’s NSI Parameters
After you complete the instrument configuration, use the Thermo Tune application to
configure the NSI source parameters. For additional information, refer to the Tune Help.
 To set the NSI source parameters
1. On the Microsoft™ Windows™ taskbar, choose Start > All Programs >
Thermo Instruments > Thermo model > model Tune, where model is your specific mass
spectrometer, to open the Tune window.
2. Click Ion Source, and then click the Ion Source tab to view the Ion Source page
(Figure 30).
The mass spectrometer automatically detects and enters the source type (NSI) in the
Ion Source Type box.
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5 Configuring the Mass Spectrometer for NSI Mode
Configuring the EASY-Spray Source’s NSI Parameters
Figure 30. Ion Source page in the Tune window for the Orbitrap Fusion (example)
3. In the Pos Ion Spray Voltage (V) box, enter 1900.
Use 1900 V as the start value for the spray voltage. If the intensity of the full-scan
spectrum is low, gradually increase the spray voltage to improve the spectrum. The
recommended range for the spray voltage is 1400–2400 kV.
4. In the Sweep Gas (Arb) box, enter 0.
5. Click Apply.
Configuring the EASY-Spray Source’s NSI Parameters
After you complete the instrument configuration, use the Thermo Tune Plus application to
configure the NSI source parameters. For additional information, refer to the Tune Plus Help.
 To set the NSI source parameters
1. Do one of the following to open the Tune Plus window (Figure 31):
• On the Windows taskbar, choose Start > All Programs > Thermo Instruments >
model > model Tune, where model is your specific mass spectrometer.
• In the Thermo Xcalibur™ application, click the Roadmap View icon, the
Instrument Setup icon, the model MS icon, and then Tune Plus.
–or–
• In the Xcalibur application, click the Roadmap View icon, the Instrument Setup
icon, and the model MS icon. Then, from the main toolbar choose model >
Start Tune Plus.
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5
Configuring the Mass Spectrometer for NSI Mode
Configuring the EASY-Spray Source’s NSI Parameters
Figure 31. Tune Plus window for an NSI-configured Velos Pro (example)
Icon for the NSI source
2. Click the NSI Source icon (or choose Setup > NSI Source) to open the NSI Source
dialog box (Figure 32).
Figure 32. NSI Source dialog box
Enter the
absolute value.
3. In the Spray Voltage (kV) box, enter 1.90.
Use 1.90 kV as the start value for the spray voltage. If the intensity of the full-scan
spectrum is low, gradually increase the spray voltage to improve the spectrum. The
recommended range for the spray voltage is 1.40–2.40 kV.
4. Click OK.
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5 Configuring the Mass Spectrometer for NSI Mode
Selecting the Source for the LCQ Deca XP Max Mass Spectrometer
Selecting the Source for the LCQ Deca XP Max Mass Spectrometer
Note This section is for the LCQ Deca XP Max mass spectrometer, which uses the
Xcalibur data system version 2.0.7 or earlier.
After you install the EASY-Spray source, use the Instrument Configuration window to
configure the mass spectrometer for NSI mode.
 To configure the mass spectrometer for NSI mode
1. If open, close the Xcalibur data system and Tune Plus application.
2. On the Windows taskbar, choose Start > All Programs > Xcalibur >
Instrument Configuration to open the Instrument Configuration window.
3. Select the devices to control from the Xcalibur data system if not already selected:
a. In the Device Types list, select All.
b. Under Available Devices, double-click the icons for the mass spectrometer and
nanoelectrospray LC instrument to add them to the Configured Devices list.
4. Double-click the mass spectrometer icon to open the Model Configuration dialog box.
5. In the left pane, select Ion Source to display the ion source configuration page, and then
select Nanospray in the Default Source list.
6. Click OK, and then click OK again to close the message box.
7. Configure the LC device if you have not done so.
For instructions, refer to the LC instrument documentation.
8. In the Instrument Configuration window, click Done.
9. Restart the data system computer and the mass spectrometer.
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6
Maintenance
This chapter provides maintenance guidelines, a list of required tools and supplies, and
instructions for replacing the controller PCB in the EASY-Spray Series source.
Contents
• Guidelines
• Preparing the Work Area
• Tools and Supplies
• Cleaning the EASY-Spray Column Emitter Tip
• Replacing the Controller PCB
Guidelines
For optimal results, follow these guidelines when performing the procedure in this chapter:
• Take precautions against electrostatic discharge (ESD), especially when the lab
environment is at the lower end of the relative humidity specification (see page xi). For
additional guidelines, refer to the “Operating Environment” chapter in the preinstallation
requirements guide for your mass spectrometer.
• Proceed methodically.
• Always place the components on a clean, lint-free surface.
• Never overtighten a screw or use excessive force.
Preparing the Work Area
 To prepare the work area
Do the following:
• Cover the area with clean, lint-free paper or a large sheet of clean aluminum foil.
• Have nearby the necessary tools, supplies, and replacement parts (when applicable).
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6
Maintenance
Tools and Supplies
Tools and Supplies
The EASY-Spray Series ion source requires very few tools to perform routine maintenance
procedures. Table 7 lists the necessary tools and supplies for maintaining the ion source and
column.
CAUTION Avoid exposure to potentially harmful materials
By law, producers and suppliers of chemical compounds are required to provide their
customers with the most current health and safety information in the form of Material
Safety Data Sheets (MSDSs) or Safety Data Sheets (SDSs). The MSDSs and SDSs must
be freely available to lab personnel to examine at any time. These data sheets describe the
chemicals and summarize information on the hazard and toxicity of specific chemical
compounds. They also provide information on the proper handling of compounds, first
aid for accidental exposure, and procedures to remedy spills or leaks.
Read the MSDS or SDS for each chemical you use. Store and handle all chemicals in
accordance with standard safety procedures. Always wear protective gloves and safety
glasses when you use solvents or corrosives. Also, contain waste streams, use proper
ventilation, and dispose of all laboratory reagents according to the directions in the MSDS
or SDS.
Table 7. Tools, equipment, and chemicals
Description
Part number
Tools and equipment
Controller PCB, with a 2.5 mm hex key and a cover
plate for use on the EASY-Spray source
ES233
EASY-Spray column wash cap
ES235
Gloves, lint-free and powder-free
Fisher Scientific 19-120-2947a
Unity Lab Services:
• 23827-0008 (size medium)
• 23827-0009 (size large)
Graduated cylinder or beaker, glass, 20–60 mm
diameter (for use with cleaning solvent)
–
Sonicator
–
Chemicals
Any organic solvent, for example:
• Acetonitrile, Optima™ LC/MS grade
• Methanol, Optima LC/MS grade
a
42
Fisher Scientific (for example):
• A955-4 (amber glass, 4 L)
• A456-4 (amber glass, 4 L)
Multiple sizes are available.
EASY-Spray Series Ion Source User Guide
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6 Maintenance
Cleaning the EASY-Spray Column Emitter Tip
Cleaning the EASY-Spray Column Emitter Tip
If the video camera’s output shows dust particles on the EASY-Spray column’s emitter tip or if
there are unresolved spray issues, follow the procedure in this section to clean the emitter.
CAUTION Wear protective gloves and eye wear when handling solvents.
 To clean the EASY-Spray column emitter
1. Carefully remove the EASY-Spray column from the source.
2. Carefully insert the column into the wash cap (Figure 33).
Figure 33. Wash cap installed over the column emitter tip
Wash cap
3. Over a wash container, hold the column and wash cap with one hand, and then slowly
pull back on the wash cap to expose the emitter tip.
4. With the other hand, gently wash the emitter tip with a stream of organic solvent.
5. Reinstall the column in the source, and then use the video camera to inspect the emitter
tip for any residual lint or particulates.
6. If the emitter tip is not fully clean, use the sonicator as follows:
a. With the column inserted into the wash cap, place the emitter tip into the graduated
cylinder or beaker.
b. Fill the container with the organic solvent to cover the emitter tip.
c. Place the container with the wash cap and column into the sonicator.
d. Hold onto these components and run the sonicator for 1 minute.
This completes the cleaning procedure. If you have further issues with the emitter, you might
need to replace the column.
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6
Maintenance
Replacing the Controller PCB
Replacing the Controller PCB
If the controller PCB becomes damaged, the actual temperature display might remain off and
the EASY-Spray column might not heat. Follow this procedure to install a new controller
PCB.
 To replace the controller PCB
1. Follow the procedure “To remove the source from the mass spectrometer” on page 17.
CAUTION High voltage. To prevent electric shock, make sure that you disconnect the
P-Bus cable before you remove the controller PCB.
2. Using the 2.5 mm hex key, remove the four hex socket-head screws located on the bottom
of the source. If you previously installed the controller PCB, P/N ES233, in the
EASY-Spray source, remove the additional cover plate (Figure 34).
Note The cover plate is for use on the EASY-Spray source (ES081) only when
installing the ES233 controller PCB.
Figure 34. EASY-Spray with the new controller PCB and extra cover plate (bottom view)
EASY-Spray housing
Controller PCB (ES233)
Cover plate
3. Slowly pull out the enclosure for the controller PCB (Figure 35).
IMPORTANT If you remove the controller PCB too quickly or at an angle, you might
damage the PCB components or the internal heater cable routed above the controller
PCB.
Figure 35. Controller PCB (P/N ES233)
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6 Maintenance
Replacing the Controller PCB
4. Unpack the new controller PCB and make sure it is not damaged.
CAUTION To prevent damage to the electronic components on the PCB due to ESD,
hold the new controller unit by the metal enclosure instead of the PCB.
5. Slowly push the new controller PCB enclosure into place, and route the heater cable so
that it does not become damaged (pinched) (Figure 36).
Figure 36. Bottom of the EASY-Spray source without the controller PCB
High voltage cable
connects to the column
holder.
One of the back holes for
the hex socket-head screw
6. (EASY-Spray only) Align the screw holes on the additional cover plate over the controller
PCB’s back two screw holes (Figure 35 on page 44).
7. Using the 2.5 mm hex key, install the four hex socket-head screws into the bottom of the
source.
You can now install the EASY-Spray Series source back onto the mass spectrometer. To
dispose of the old controller PCB, see “WEEE Directive 2012/19/EU” on page iii.
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6
Maintenance
Replacing the Controller PCB
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7
Troubleshooting
Table 8 lists some EASY-Spray Series ion source and column problems, their causes, and their
possible solutions. You can resolve most problems by using a new EASY-Spray column.
If you need further troubleshooting assistance, contact your local Thermo Fisher Scientific
field service engineer. See “Contacting Us” on page xv.
Contents
• Camera issues
• Column issues
• Signal issues
Table 8. EASY-Spray Series source problems, causes, and possible solutions (Sheet 1 of 3)
Problem
Cause
Solution
The camera light is off.
Turn on the camera by pressing its top light button
(Figure 18 on page 20).
The heater is off, or there might
be a loose connection or a
damaged PCB.
Try these solutions:
Camera issues
Camera issues:
The light is not on.
–or–
The video output
is too dark.
Column issues
Heater issues:
The column is
not heated.
• Check all of the connections for the controller power
supply unit from the electrical wall outlet to the
EASY-Spray Series source.
–or–
The actual
temperature
display is blank.
Thermo Scientific
• Turn on the heater by selecting a temperature setting.
• See “Replacing the Controller PCB” on page 44.
The EASY-Spray column is
damaged.
Replace the EASY-Spray column.
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7
Troubleshooting
Table 8. EASY-Spray Series source problems, causes, and possible solutions (Sheet 2 of 3)
Problem
Cause
Solution
The parameters for the LC, MS,
or both might need adjusting.
Try these solutions:
Signal issues
The signal
intensity is weak.
• Verify that the EASY-Spray column is correctly
positioned (that is, clicks on insertion).
• Check the LC method and MS tune method
parameters.
• Run a known standard to check the sensitivity.
The mass spectrometer’s spray
cone and ion transfer tube are
dirty.
Clean both the spray cone and ion transfer tube. For
instructions, refer to the mass spectrometer’s
documentation.
The spray is
unstable.
There is a leak somewhere in
the liquid path.
Check all of the LC connections.
–or–
Air bubbles in the emitter might
cause the spray to “spit.”
Try degassing the mobile phase or purging the line, and
then recheck the line for air bubbles.
There is no spray.
There is an emitter blockage
from particles in the sample,
other small particles from the
flow lines or valves, and so on.
Try adjusting the spray voltage. If that does not resolve the
blockage problem, either clean the emitter (see page 43) or
insert a new EASY-Spray column.
The cleaning solvent is not
Use an LC/MS-grade solvent to clean the emitter tip.
LC/MS grade, which can
negatively affect the spray stability.
The EASY-Spray column is out
of alignment, which might occur
if you bumped the source or
moved the source from one MS
to another MS.
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EASY-Spray Series Ion Source User Guide
Follow the procedure “Adjusting the Emitter Tip Position”
on page 15.
Thermo Scientific
7
Troubleshooting
Table 8. EASY-Spray Series source problems, causes, and possible solutions (Sheet 3 of 3)
Problem
Cause
Solution
(continued)
The source high-voltage (HV)
connection might be unstable.
Check the high voltage contact on the back of the source
(Figure 9 on page 10 and Figure 10 on page 11) and the
front of the mass spectrometer.
If the problem continues for the EASY-Spray NG source,
do the following:
1. Open Tune and view the Ion Source page (see
page 38).
2. Compare the setting for the spray voltage to the
adjacent readback value.
A green box ( ) indicates that the parameter is
functioning properly.
If the problem continues for the EASY-Spray source, do the
following:
1. Open Tune Plus, choose View > Display Status View,
and then click the All tab.
2. Choose Setup > NSI Source to open the NSI Source
dialog box.
3. Compare the setting in the NSI Source dialog box to
the readback value for the NSI source’s absolute spray
voltage.
A green check mark (
) indicates that
the parameter is functioning properly.
The source’s HV connector has a
loose connection.
If you have a digital multimeter that can measure 20 M
(mega ohms), do the following:
1. Test the meter’s batteries, and then set the meter to the
appropriate resistance setting or range.
2. Measure the resistance from the HV pin on the back of
the source to the spring-loaded bolt located inside the
housing (Figure 9 on page 10, or Figure 10 on
page 11).
A functional HV connection has a measured resistance
of 18–22 M,.
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8
Replaceable Parts
This chapter lists the consumable and replaceable parts for the EASY-Spray Series ion sources,
and their corresponding part numbers. To order any of these parts, contact your local
Thermo Fisher Scientific field service engineer or go to www.proxeon.com/easyspray.
Contents
• Spare Parts
• Consumables
Spare Parts
A/B mixing/venting tee . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
EASY-Spray controller PCB (with a 2.5 mm hex key and cover plate) . . . . . . . . . . .
EASY-Spray controller power supply with power adapter cable . . . . . . . . . . . . . . . . .
EASY-Spray tools: emitter positioning tool and hex keys (1.5 mm and 3 mm) . . . . .
Monitor and power supply unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Tee holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Video camera and power supply unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Wash cap (for the EASY-Spray column) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
SC901
ES233
ES231
ES232
ES217
ES234
ES216
ES235
Consumables
Peptide Columns
EASY-Spray column, 15 cm × 75 μm ID, PepMap™ C18, 3 μm particles,
100 Å pore size . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
EASY-Spray column, 15 cm × 50 μm ID, PepMap RSLC C18, 2 μm particles,
100 Å pore size . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
EASY-Spray column, 25 cm × 75 μm ID, PepMap RSLC C18, 2 μm particles,
100 Å pore size . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
EASY-Spray column, 50 cm × 75 μm ID, PepMap RSLC C18, 2 μm particles,
100 Å pore size . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Thermo Scientific
ES800
ES801
ES802
ES803
EASY-Spray Series Ion Source User Guide
51
8
Replaceable Parts
Consumables
Intact Protein Columns
EASY-Spray column, 25 cm × 200 μm ID, PepSwift™ Monolith . . . . . . . . . . . . . . . ES810
EASY-Spray column, 15 cm × 75 μm ID, Accucore™ C4, 2.6 μm particles,
150 Å pore size . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ES811
EASY-Spray column, 15 cm × 75 μm ID, PepMap C18, 3 μm particles,
300 Å pore size . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ES812
52
EASY-Spray Series Ion Source User Guide
Thermo Scientific
G
Glossary
A
B
C
D
E
F
G
H
I
J
K
L M N O
P
Q
R
S
T
U
V W X
Y
Z
A
electrospray ionization (ESI) See electrospray (ESI).
API ion transfer tube A tube assembly that assists in
desolvating ions that are produced by the ESI,
nanoESI (NSI), or APCI probe (or spray insert).
H
atmospheric pressure chemical ionization (APCI) A
soft ionization technique done in an ion source
operating at atmospheric pressure. Electrons from a
corona discharge initiate the process by ionizing the
mobile phase vapor molecules. A reagent gas forms,
which efficiently produces positive and negative ions
of the analyte through a complex series of chemical
reactions.
atmospheric pressure ionization (API) Ionization
performed at atmospheric pressure by using
atmospheric pressure chemical ionization (APCI),
electrospray (ESI), or nanoelectrospray ionization
(nanoESI or NSI).
heated-electrospray (HESI) A type of atmospheric
pressure ionization that converts ions in solution into
ions in the gas phase by using electrospray (ESI) in
combination with heated auxiliary gas.
heated-electrospray ionization (H-ESI) See heatedelectrospray (HESI).
I
ion source A device that converts samples to gas-phase
ions.
ion sweep cone A removable cone-shaped metal cover
that fits on top of the API ion transfer tube and acts
as a physical barrier to protect the entrance of the
tube.
D
dynamic nanoelectrospray See nanoESI nanoLC
gradient separation.
E
N
nano liquid chromatography (nanoLC) Liquid
chromatography with typical flow rates of
10–1000 nL/min and 10–150 μm diameter
columns.
electrospray (ESI) A type of atmospheric pressure
ionization that is currently the softest ionization
technique available to transform ions in solution into
ions in the gas phase.
Thermo Scientific
EASY-Spray Series Ion Source User Guide
53
Glossary: nanoelectrospray ionization (nanoESI or NSI)
nanoelectrospray ionization (nanoESI or NSI) A
type of electrospray (ESI) that accommodates very
low flow rates of sample and solvent on the order of
1–20 nL/min (for static nanoelectrospray) or
100–1000 nL/min (for dynamic nanoelectrospray,
which is also called nanoESI nanoLC gradient
separation).
nanoESI nanoLC gradient separation Employs
microscale capillary columns to separate the analytes
in complex mixtures. The sample is loaded onto a
column using an injection valve or a gas pressure
vessel. The mixture components are then eluted by a
solvent gradient and pumped through the emitter.
nanoESI (NSI) source The entire nanoelectrospray
ion source.
nanoESI (NSI) spray current The flow of charged
particles in the nanoESI (NSI) source. The voltage
on the NSI spray needle supplies the potential
required to ionize the particles.
nanoESI (NSI) spray voltage The high voltage that is
applied to the spray needle in the nanoESI (NSI)
source to produce the NSI spray current as liquid
emerges from the nozzle. The NSI spray voltage is
selected and set; the NSI spray current varies.
nanospray ionization (NSI) See nanoelectrospray
ionization (nanoESI or NSI).
S
source See ion source.
static nanoelectrospray A device that performs
continuous analysis of small analyte solution volumes
over an extended period of time.
54
EASY-Spray Series Ion Source User Guide
Thermo Scientific
I
Index
A
axes control
x- and z- 16
y- 17
C
camera
connecting 19
light button and focusing wheel, locations 20
removing 18
troubleshooting 47
cleaning, emitter tip 43
column
assembly 25
cleaning 43
installing 24–25
part numbers for
intact protein 52
peptide 51
setting the temperature 25
consumables 51
contacting us xv
controller PCB
description 4
power supply for 5
replacing 44
D
directive, WEEE iii
documentation
accessing x
additional x
downloading x
E
EASY-Spray column
See column
Thermo Scientific
EASY-Spray P-Bus Temp Control Cable
connecting 13
description 5
EASY-Spray Series ion source
See ion source
electrical wall outlets 7
emitter
adjusting the tip position 16
tip, cleaning 43
troubleshooting 48
environmental requirements xi
F
figures, list of vii
H
heater
cable 25
troubleshooting 47
high voltage
connector, location 10–11
internal cable, location 45
troubleshooting 49
housing
See ion source
I
ion source
axes control
x- and z- 16
y- 17
benefits 1
column, installing 25
compatible mass spectrometers xi
connecting
controller power supply unit 12
EASY-Spray P-Bus Temp Control Cable 13
EASY-Spray Series Ion Source User Guide
55
Index: K
monitor and camera 19
Tee holder 13
housing
description 3
installing 9
kits xii
locking levers, positions 10
power connection 12
removing 17
ion sweep cone 9
NSI mode
manual configuration, LCQ Deca XP Max 40
setting the spray voltage 39
NSI Source dialog box 39
O
organic solvents 42
P
kits
EASY-Spray Ion Source xii
EASY-Spray NG Ion Source xii
UltiMate 3000 RSLCnano EASY-Spray Application 33
power connection, location 4
power supply units, connecting
camera 19
controller 12
monitor 19
product requirements xi
L
R
LC connections
EASY-nLC 1000, connecting to 27
EASY-nLC II, connecting to 30
RSLCnano system, connecting to 33
tools and supplies 23
locking levers, positions 9
replaceable parts 51
K
M
maintenance
controller PCB, replacing 44
guidelines 41
tools, equipment, and chemicals 42
work area, preparing 41
mass spectrometer
diagnostics 49
ion sweep cone 9
NSI mode manually, LCQ Deca XP Max 40
power requirements xi
preparing for the source 8
setting the NSI source parameters 37
source, removing 17
spray cone 10
monitor
connecting 19
video picture, adjusting 21
MSDS 42
N
nanoelectrospray, advantages of 2
nanoESI
See NSI mode
nanoViper fittings, using 26
56
EASY-Spray Series Ion Source User Guide
S
SDS 42
signal intensity, troubleshooting 48
source
See ion source
spray
troubleshooting 48
voltage
readback value 49
setting the value, EASY-Spray 39
setting the value, EASY-Spray NG 38
spray cone, MS 10
T
Tee holder
adjusting position 28
configuring 14
installing 13
shipping configuration 14
temperature
control dial, settings 4
display codes 26
display location 4
temperature control cable, connecting 13
tools and supplies, connecting the LC 23
troubleshooting 47
Tune
application, opening 37
legacy application, opening 38
Thermo Scientific
Index: V
V
venting tee, recommended LC connections 28
W
wash cap, installing 43
WEEE directive iii
X
x, y, and z coordinates, column holder 16
Thermo Scientific
EASY-Spray Series Ion Source User Guide
57
Index: X
58
EASY-Spray Series Ion Source User Guide
Thermo Scientific
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