Lambda X65 series
Lambda X65 series
UV/Vis Simplified for your Lab
Gerlinde Wita
October 2015
KEY MESSAGES
• More than 50 years history of winning UV-Vis
technology by...
• THE HEART OF LAMBDA IN A SMALLER AND
INNOVATIVE PACKAGE
• 30,000 customers can’t be wrong
• LAMBDA means QUALITY!
• We offer the broadest range of UV-Vis-NIR
spectrometers – just tell us what you need and we will
deliver the right instrument
2
More than 50 years history of winning UV-Vis
technology by PerkinElmer
• 1937
◦ Richard S. Perkin und Charles W. Elmer
found the Perkin-Elmer optical shop
• 1962
◦ Perkin-Elmer first UV-Vis instrument UV 139
• 1981
◦ PerkinElmer first LAMBDA UV-Vis system
R.S. Perkin
3
C.W. Elmer
35 years Lambda UV-Vis spectrometer
UV/Vis/NIR product portfolio
Lambda HighPerformance UV/Vis NIR
Lambda 650/750/850/950/1050
Performance
PerkinElmer UV software
UV Winlab for HPUV
UV Express for Lambda 365
UV Lab for Lambda 265/465
Lambda 465
UV/Vis/NIR-Spectrometer
High performance across the
UV/Vis/NIR range
Unmatched flexibility and sensitivity
Lambda 365
UV/Vis-Spectrometer
High performance PDA that delivers
reliability – and confidence
Lambda 265
UV/Vis-Spectrometer
compact, versatile high-performance
double-beam UV/Vis
PDA UV/Vis-Spectrometer
fast, accurate, affordable results
Lambda XLS/Bio
4
UV/Vis-Spectrometer – stand alone
easy, fast, robust and reliable
..the broadest product portfolio - for each application the right solution
Which Model to choose?
Conventional
(“Dispersive”)
LAMBDA
365
LAMBDA
35
HPUV
LAMBDA
5
Photodiode Array (PDA)
LAMBDA
XLS/ BIO
LAMBDA
265
LAMBDA 465
tell us your needs and we recommend the right system..
UV/Vis simplified for the Lab …..
PerkinElmer’s new LAMBDA series offers confidence in a wide range of sample analyses.
Self-aligning accessories provide for “mistake proof” materials characterization.
• Intuitive Software with a simplified user interface
• Instrument self check, never a question about performance
• Flexibility to rapidly change sample analysis
• Automated Accessory Align
• Compact Design, saves valuable bench space
• Application modules covering many application areas to save time
• Advanced Visualization Tools
6
Lambda UV-Vis for Improving the Quality Life
Foods/Nutritionals
Industrial
Packaging
Characterization
Color Testing
Vitamins
Wine testing
Fuels
Pharma/Life
Sciences
Environmental
Air/Water/Soil
Color Testing
RNA/DNA Studies
Multi-Component
Analysis
Enzyme Kinetics
Heavy Metals in
Water
Dissolution testing
Nano Particles
USP Method
Compliance
Soil
Contamination
Solvent Analysis
Textiles
Catalysis
7
Sunscreens
LAMBDA 365
Work Horse Double Beam UV/Vis
Lambda 365
Compact, versatile high-performance double-beam UV/Vis
The LAMBDA 365 delivers state-of-the-art UV/Vis performance
that meets the needs of pharmaceuticals, analytic chemists,
geneticists, and manufacturing QA/QC analysts everywhere.
With 21 CFR part 11 software available, the LAMBDA 365 is
ready to support all your needs – everything from standard
methods and applications to those requiring regulatory
compliance.
THE HEART OF LAMBDA IN A SMALLER AND INNOVATIVE PACKAGE
30,000 customers can’t be wrong
LAMBDA means QUALITY!
Send your old Lambda into retirement
and replace it with the NEXT generation!
9
Lambda 365
Compact, versatile high-performance double-beam UV/Vis
• Ready to support all your needs
• Double-beam technology - when
high stability and low stray light are
critical
• Large sample compartment
• Wide range of accessories
• Easy-to-install accessories to
minimize setup time and effort
• Auto-alignment of multi cell
changers
For optimized results in a wide range of routine applications,
including manufacturing and pharmaceutical QA/QC,
environmental testing, academics and more
10
Lambda 365
Compact, versatile high-performance double-beam UV/Vis
Lambda 365 Double Beam UV/VIS Spectrophotometer
•
•
•
•
•
•
Includes
•
Accessories To Support Key Applications
•
•
•
11
Double Beam Optics (Dual Silicon
Photodiode detectors)
Wavelength Range: 190 - 1100 nm
Dynamic range to 4A
Wavelength Accuracy: ±0.1 nm (at D2 peak
656.1 nm)
Spectral Bandwidth: Variable (0.5, 1, 2, 5, 20
nm)
Interface: USB
UV Express software
•
Easy to learn and use software
•
Method based with calculations and report
•
Wavelength Program
•
Scan
•
Quant
•
Kinetics
•
Scanning Kinetics
•
Bio Applications (Protein / DNA / RNA analysis,
DNA Melting)
•
Windows 7 and 8 compatible (32/64 bit)
Single Cell Holder 10 mm
USB Cable for Communication
User's Guide
The Heart of a Lambda in a Smaller Package
Performance comparison
Parameter
Lambda 365
Lambda 25
Lambda 35
Optics
Double Beam Optics
Double Beam Optics
Double Beam Optics
Detectors
Dual Silicon Photodiode
Photodiode
Photodiode
Size (W x D x H)
495 x 500 x 270 mm
650 x 560 x 233 mm
650 x 560 x 233 mm
USB Connectivity as standard
Yes
No
No
Built-in diagnostics
Enhanced with software start
With power on
With power on
Microsoft Windows® 7 and 8
compatible
Yes
No
No
Spectral Bandwidth
Variable (0.5, 1, 2, 5, 20nm)
Fixed (1nm)
Variable (0.5, 1, 2, 4nm)
PhEUR Stray Light (@198nm)
>2A
>2A
>2A
Stray Light (ASTM)
<0.02%T
<0,01%T
<0,01%T
Absorbance Stability
0.0003A/hour
0.00015 A/hour
0.00015 A/hour
Absorbance Range
+/- 4A
+/- 3.2A
+/- 3.2A
Minimum spectral resolution
0.5nm
0.5nm
0.5nm
Wavelenght range
190-1100nm
190-1100nm
190-1100nm
Fastest scan speed
3000 nm/min
2880 nm/min
2880 nm/min
Appearance/General Features
Performance
12
LAMBDA 365 offers REAL stability by design
• The thick cast metal
baseplate used by PerkinElmer
offers unrivalled robustness
(especially when comparing
with others using pressed
metal or plastic designs).
• It also contributes to our high
data stability. The instrument is
not affected by thermal
effects in the lab.
• The large base allows to
accommodate our large, high
performance optics design for
excellent results.
Lambda 365 optical beam path
13
Lambda 365 offers a wide range of accessories and
configurations
Lambda 365 accessories
For liquids
1+1 cell
1+1 cell (jacketed)
1+1 Variable pathlength cell
1+1 Test tube holder
8+1 cell changer
8+1 cell changer (jacketed)
1+1 cell (Peltier)
6+1 cell changer (Peltier)
Sipper
•
Autosampler
Fiber probe
For solids
1+1 Transmission
•
The LAMBDA 365 has a wide range of
accessories for any kind of application with
liquid or solid samples.
Customer ease of use, e.g.
◦
1+1 Film holder
Variable angle transmission
1+1 Reflectance
Reflectance 6°
Variable angle reflectance
1+1 Integrating sphere
14
◦
◦
◦
Accessories are pre-aligned and snap right in without
tools
Cell changers self align automatically.
All tubing and cables inside jacketed cell holders or
changers are completely pre-installed.
The liquid connection is done by self-sealing snap in
connectors placed outside the instrument.
Lambda 365 Accessory examples:
Cell and Sample Holder for transmission and reflectance
1+1 Microsampling Cell Holder
The ideal solution for samples smaller than the instrument beam size
• Demagnifies the beam
• Increasing energy throughput
1+1 Advanced Transmission Sample Holder
• Designed to hold materials such as glass, polymers, and films
• Providing a positive locating point for the sample that can be adjusted to
accommodate a range of sizes and thicknesses.
1+1 Fixed Angle Reflectance Accessory
• Easy-to-use specular reflectance accessory for analysis of solid samples
• Provides high-quality data for identifying films and coatings, measurement
of the film thickness, and surface studies of metals.
1+1 Variable Angle Transmission Accessory
• Allows reproducible measurement at multiple angles without moving the
mounted sample.
• The angle is easily set by rotating the sample mounting stage.
1+1 Variable Pathlength Cuvette Holder
An easy to install accessory for rectangular cells of varying pathlength.
• The partition plate allows cells to be supported throughout analysis
• Accommodates 5, 10, 20, 40, 50, and 100 mm pathlength cells
15
... see PDL for more options
Lambda 365 Accessory examples:
Cell changer and Peltier
Automated 8+1-Cell Changer
• fully controlled via software, with parameters stored in the
application method.
• simple to change in seconds – no tools necessary.
Single Cell or 6+1-Position Peltier Controlled Cell Changer,
Controller (with or without Heated Reference)
This six-cell holder allows near synchronous data collection across six conditions in
a single experiment, with unattended operation. It is useful for thermal denaturation,
kinetics, and other applications where precise temperature control is necessary
• Accommodates up to 6 samples for automated measurement
• Full software control of temperature and stirring
• Contains a built-in radiator that maintains temperature of circulating coolant
• Includes a Peltier 6-Position Multi-Cell Holder and 2 temperature probes
• Temperature Control: Sample only
• Temp. Control Range: -10 to 100°C
• Temperature Accuracy: ± 0.15°C
• Temperature Control Precision: 0.1°C
16
... see PDL for more options
Lambda 365 Accessory examples:
Sipper and integrating sphere
Auto Sipper Accessory
consists of Automatic Peristaltic Pump, Pumping Cartridge, tubing, and Computer
Interface Cable.
• complete software control for automation of liquid sampling
• Flow of the sample is controllable by the software
• 2 flow channels, and included is 1 flow-through cell, 420 μL (SC-2)
• Speed (RPM): 1.6 to 160
• Flow Rate: 0.003 mL/min to 68.8 mL/min
• Tubing I.D.: 0.13 to 3.17 mm
• Operating Temperature Range: 0 to 40°C
50 mm Transmission/Reflectance Sphere, High Sensitivity
Used for measuring the diffuse reflectance (SCE), total transmittance, diffuse transmittance, and performing
color analysis.
•
Spectral range of 230-1100 nm (UV and visible).
•
port size is 10 mm
•
Spectral bandwidth is 20 nm
•
White Standard included
17
... see PDL for more options
LAMBDA 265
Fast, accurate, affordable results
18
Lambda 265 – Fast, accurate, affordable results
With ultrafast data processing and maximum reliability, the LAMBDA 265 is
the ideal system for a wide range of R&D and QA/QC applications, all while
taking up minimal bench space. Its photodiode array (PDA) detector
enables data to be acquired simultaneously across the full wavelength
range – from 190 nm to 1100 nm. In seconds, your processing is complete
and ready for you to act on. Plus, the LAMBDA 265 system’s robust
modular design, with no moving parts, is ideal for any busy lab. The high
energy Xenon flash lamp is active only when a spectrum is being acquired
and provides years of worry-free operation. And the system’s compact size
makes it simple to move it to any location where it might be needed
19
Lambda 265 – Fast, accurate, affordable results
• High energy Xenon flash lamp is active only
when a spectrum is being acquired and
provides years of worry-free operation
• Open sample area for easy sample access
and visibility
• Small footprint and no moving parts make it
ideal for a wide range of applications
• Range of accessories for liquids and solids
Ease of use, ultrafast data processing and maximum reliability, the
LAMBDA 265 is the ideal system for multi user labs (e.g.
Academia/Teaching), Biotech (fast and special accessories for DNA
melting experiments) and a wide range of other applications, all while
taking up minimal bench space
20
Lambda 265 Personal PDA
Full Spectral Range in A Flash
Lambda 265 Photodiode-Array UV/Vis Spectrophotometer
•
•
•
•
•
•
•
190-1100 nm Wavelength
Fast spectral acquisition
2nm Spectral Resolution,
± 1nm Wavelength Accuracy
Xenon Flash Lamp
512 element Photodiode Array
Up to 50 mm pathlength cuvette capability
Includes
•
•
•
•
•
•
•
•
•
•
•
•
21
UV Lab Software
System Control / Data Processing
Scan Mode
Wavelength Monitoring
Film Thickness
Quant Mode
Kinetics Mode
Windows 7and 8 compatible
Single Cell Holder, 10 mm
USB Cable for Communication
User's Guide
AC Power Cord
Diode Array Technology – Lambda 265 Design
22
•
The diode array spectrometer configuration
for the LAMBDA 265 utilizes a reverse optic
design passing white light through the
sample. As the lamp is pulsed a dark
measurement to be made. This is used to
correct for ambient light, allowing sampling
to be done without the need of a sample
compartment cover. Diode array
spectrometers allow full spectrum to be
acquired for each scan cycle (190nm 1100nm ). The grating separates the poly
chromatic light into discrete wavelengths
which are focused on the linear array.
•
The LAMBDA 265 utilizes a Xenon flash lamp
providing long life as the lamp is only flashed
during a data acquisition.
Lambda 265 – Fast, accurate, affordable results
Some performance data
Parameter
Lambda 265
Appearance/General Features
Source Lamp
Xenon Flash
Detectors
Diode Array
Size (W x D x H)
340 x 320 x 115 mm
USB Connectivity as standard
Yes
Microsoft Windows® 7 and 8
compatible
Yes
Performance
23
Spectral Bandwidth
2nm (Spectral resolution)
Stray Light
<1.0% (198 nm KCL)
<0.05% (220 nm Nal)
<0.03% (340 nm NaNO2)
Wavelenght Accuracy
+/- 1nm
Wavelength Reproducibility
+/- 0.02nm
Absorbance Range
+/- 3A
Photometric Stability
< 0.002 AU/h @ 0 AU, 300 nm
Wavelenght range
190-1100nm
Lambda 265 offers a wide range of accessories
and configurations
Lambda 265 accessories
For liquids
Single cell
Single cell (jacketed)
Variable pathlength cell
8 cell changer
8 cell changer (jacketed)
Single cell (Peltier)
•
Compared to other DAD systems of its size,
the LAMBDA 265 offers a wide range of
accessories for a wide range of application
with liquid or solid samples.
•
Plus, most of these accessories snap right in
– no tools necessary – so everyone in your
lab can be productive right away
6 cell changer (Peltier)
Sipper
Autosampler
For solids
Transmission
Film holder
Reflectance
24
Lambda 265 Accessory examples:
Cell and Sample Holder for transmission and reflectance
Water Jacketed Single Cell Holder
Maintains temperature control over standard 10 mm cells using recirculating
liquid
Variable Pathlength Holder
An easy to install accessory for rectangular cells of different pathlength. The partition
plate allows cells to be supported throughout analysis.
It accommodates 5, 10, 20, and 50 mm pathlength cells.
Reflectance Module Accessory
Designed for measuring specular reflectance spectra at a fixed
angle (incident angle: 30° fixed). It is used for film thickness
measurement.
Advanced Transmission Holder
This is useful for transmission measurements on various solid
samples (films, filters, glass, plastics, etc.). The spring-loaded
clamp securely holds objects up to 2.5 cm thick. The magnetic
plate precisely positions films and thin samples
25
... see PDL for more options
Lambda 265 Accessory examples:
Cell changer and Peltier
Water Jacketed 8-Position Multi-Cell Holder
Accommodates any standard 10 mm cells (up to 8 cells), while using
recirculating liquid to control the temperature of multi samples for
simultaneous analysis. Also comes with automated software calibration
Peltier Controlled Single Cell
Useful for thermal denaturation, kinetics and other applications where precise
temperature control is necessary. It comes with full software control of temperature
and stirring for 1 sample. Also has a built-in radiator that maintains temperature of
circulating coolant. Includes a Peltier Single Cell Holder and 2 temperature probes.
•
Temp. Control Range: - 10 to 100°C
•
Temperature Accuracy : ± 0.15°C
•
Temperature Control Precision : 0.1°C
26
... see PDL for more options
LAMBDA 465
High performance PDA that delivers reliability
– and confidence
27
Lambda 465
High Performance PDA that delivers reliability – and confidence
Designed specifically for high-end research as well as routine and highthroughput applications, the LAMBDA 465 is the innovative PDA solution
that provides maximum reliability – for maximum confidence in your results.
Its PDA technology allows the acquisition of a full spectrum – from 1100 nm
to 190 nm – in as little as 20 msec. In addition, the system has 1-nm
resolution, allowing it to meet the requirements of a number of
pharmacopoeias. With 21 CFR part 11 compliant software, it’s an ideal
solution for dissolution, fast kinetics, and other applications where highspeed scanning and high resolution are required – and it’s perfect for
method development and sample analysis, too.
28
Lambda 465
High performance PDA that delivers reliability – and confidence
• Photodiode array (PDA) detector enables
data to be acquired simultaneously across the
full wavelength range
• Dual light source (tungsten and deuterium) in
a see-through configuration is unitized. This
configuration provides the highest energy
throughput possible – an important
consideration with accessories that impact
energy throughput
• Open sample area for easy sample access
and visibility
• Range of accessories for liquids and solids
It’s an ideal solution for dissolution, fast kinetics, and other of
applications where high-speed scanning and high resolution are
required – and it’s perfect for method development and sample
analysis, too.
29
Lambda 465
High performance PDA that delivers reliability – and confidence
LAMBDA 465 Photodiode-Array UV/Vis Spectrophotometer
•
190-1100 nm spectral range,
•
0.95nm SBW ( Pharma compliant )
•
Ultra-fast scanning time : 20 msec,
•
1024-Channel Photodiode array detector
•
21 CFR part 11 package available
Includes
30
•
UV Lab Software
•
General System Control / Data Processing
•
Quantification
•
Material Thickness Measurement
•
System Monitoring
•
Advanced Diagnostics
•
Single Cell Holder, 10 mm
•
USB Cable for Communication
•
User's Guide
•
AC Power Cord
Diode Array Technology – Lambda 465 Design
•
•
31
The diode array spectrometer configuration for
the LAMBDA 465 utilizes a reverse optic design
passing white light through the sample. As the
lamp is blocked by a shutter a dark
measurement to be made. This is used to
correct for ambient light, allowing sampling to
be done without the need of a sample
compartment cover. Diode array
spectrometers allow full spectrum to be
acquired for each scan cycle (190nm -1100nm
). The grating separates the poly chromatic
light into discrete wavelengths which are
focused on the linear array.
The LAMBDA 465 uses a more conventional D2
/ Tungsten halogen lamp configuration. This is
also the same basic lamp combination used
on the LAMBDA 365 as well as all the LAMBDA
25/35/45 instruments. The advantage of this
lamp configuration is achievement of the
highest energy levels across the complete
UV/Vis range
Lambda 465
High performance PDA that delivers reliability – and confidence
Parameter
Lambda 465
Appearance/General Features
Source Lamp
Tungsten/Deuterium
Detectors
Diode Array 1024 channel
Size (W x D x H)
541 x 450 x 232 mm
USB Connectivity as standard
Yes
Microsoft Windows® 7 and 8
compatible
Yes
Performance
32
Spectral Bandwidth
0,95 nm (Spectral Resolution)
Stray Light
<1.0% (198 nm KCL)
<0.05% (220 nm Nal)
<0.03% (340 nm NaNO2)
Wavelenght Accuracy
+/‐0 .5 nm (Holmium Oxide
Solution
NIST 2034)
+/‐ 0.2 nm (D2 peak, 486.0 nm
and 656.1 nm)
Wavelength Reproducibility
+/- 0.02nm
Absorbance Range
+/- 3A
Photometric Stability
< 0.001 AU/h @ 0 AU, 300 nm
Wavelenght range
190-1100nm
Lambda 465
offers a wide range of accessories and configurations
Lambda 465 accessories
For liquids
Single cell
Single cell (jacketed)
Variable pathlength cell
8 cell changer (jacketed)
Single cell (Peltier)
6 cell changer (Peltier)
Sipper
Fiber probe
•
The LAMBDA 465 offers a wide range of
accessories for a wide range of application
with liquid or solid samples.
•
Plus, most of these accessories snap right in
– no tools necessary – so everyone in your
lab can be productive right away
Autosampler
For solids
Transmission
Variable angle transmission
Film holder
Diffuse reflectance
Reflectance
33
Lambda 465 Accessory examples:
Cell and Sample Holder for transmission and reflectance
Water Jacketed Single Cell Holder
Maintains temperature control over standard 10 mm cells using recirculating
liquid
Variable Pathlength Cell Holder, single cell
An easy to install accessory for rectangular cells of different pathlength. The partition
plate allows cells to be supported throughout analysis.
It accommodates 5, 10, 20, 40, 50 and 100 mm pathlength cells.
Reflectance Holder
Designed for measuring specular reflectance spectra at a fixed
angle (incident angle: 34° fixed). It is used for film thickness measurement.
Variable Angle Transmission Holder
For transmission measurements of various samples of film, glass and plate
etc. at various angles. It adjusts the angle of solid
samples for transmission measurements (0~306°). Also accommodates
vairous thickness samples and large samples
34
... see PDL for more options
Lambda 465 Accessory examples:
Rapid Mixing Accessories and Fiber Optic Probe
Fiber Optic Probe/Module
Used for measuring liquid samples; intended for the hazardous
samples. Includes a transmission dip probe and a 10 mm pathlength
tip. Easy to clean.
Rapid Mixing Accessory
For monitoring kinetics reactions in solution by stopped-flow
technique. Dead time : 8 ms/Optical Pathlength : 2 and 10 mm
Min. Vol./Shot/Reagent: 20 μL. Ratio mixing: up to 10:1 by using
different sized syringes.
Rapid Mixing Accessory w/Pneumatic Drive
For monitoring kinetics reactions in solution by stopped-flow
technique. Dead time : 8 ms/Optical Pathlength :2 and 10 mm
Min. Vol./Shot/ Reagent : 20 μL. Ratio mixing : up to 10:1 by
using different sized syringes. Includes pneumatic drive which
allows push button operation and best accuracy for zero time
measurements.
35
... see PDL for more options
Lambda 465 Accessory examples:
Cell changer and Peltier
Water Jacketed Automatic Referencing Stage (e.g. sample shuttle)
Accommodates any standard 10 mm cells 2-Position for reference and
sample. Unique accessory provides long term stability for monitoring kinetics
reactions. Use recirculating liquid to control the temperature of a sample
and reference solution for simultaneous analysis
Peltier Controlled Multi-Cell (8) with Peltier Controller
Useful for thermal denaturation, kinetics and other applications where precise
temperature control is necessary. It accomodates up to 8 samples for automated
measurement. Full software control of temperature and a built-in radiator that
maintains temperature of circulating coolant. Includes a Peltier 8-position multi-cell
holder and 2 temperature probes.
•
Temp. Control Range: - 10 to 100°C
•
Temperature Accuracy : ± 0.15°C
•
Temperature Control Precision : 0.1°C
36
... see PDL for more options
Nano Sticks
Each Nano Stick standard package kit (with case) comes with 1 Nano Stick, 1 loading plate, 1 bubble
checker, and a user guide. The preferred color of your choice can be selected (Black, Blue, Red, Gold,
or Silver). The Z-Dimension, the height of the center of the light beam, is 15 mm. The beam
pathlength is 0.5 mm. Fits standard 10 mm cell holder
37
... see PDL for more options
Was this manual useful for you? yes no
Thank you for your participation!

* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project

Download PDF

advertisement